SUIT-003 O2 ce D028: Difference between revisions
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::: '''[[Categories of SUIT protocols |SUIT- | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' RLE(S) | ||
::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP) | ::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP) | ||
::: '''[[Mark names in DatLab]]:''' CCP(S)R2 | ::: '''[[Mark names in DatLab]]:''' CCP(S)R2 | ||
== R,L,E format == | == R,L,E format == | ||
Revision as of 15:34, 16 March 2017
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 O2 ce D028
Description
Abbreviation: RLE(S)
Reference: A CCP_RP2
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-category: RLE(S)
- SUIT protocol pattern: linear coupling control protocol (CCP)
- Mark names in DatLab: CCP(S)R2
R,L,E format
In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states.
- 3Rot,4S;5Ama
- 3Rot: In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A (CCP02-Rot,Ama.
- 4S: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated.
- 5Ama: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).
