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Yao 2016 Biochem Biophys Res Commun

From Bioblast
Publications in the MiPMap
Yao K, Zhang WW, Yao L, Yang S, Nie W, Huang F (2016) Carvedilol promotes mitochondrial biogenesis by regulating the PGC-1/TFAM pathway in human umbilical vein endothelial cells (HUVECs). Biochem Biophys Res Commun 470:961-6.

» PMID: 26797282

Yao Kai, Zhang Wayne W, Yao Luyu, Yang Shu, Nie Wanpin, Huang Feizhou (2016) Biochem Biophys Res Commun

Abstract: Carvedilol, a third-generation and nonselective β-adrenoceptor antagonist, is a licensed drug for treating patients suffering from heart failure in clinics. It has been shown that Carvedilol protects cells against mitochondrial dysfunction. However, it's unknown whether Carvedilol affects mitochondrial biogenesis. In this study, we found that treatment with Carvedilol in HUVECs resulted in a significant increase of PGC-1α, NRF1, and TFAM. Notably, Carvedilol significantly increased mtDNA contents and the two mitochondrial proteins, cytochrome C and COX IV. In addition, MitoTracker Red staining results indicated that treatment with Carvedilol increased mitochondria mass. Mechanistically, we found that the effect of Carvedilol on the expression of PGC-1α is mediated by the PKA-CREB pathway. Importantly, our results revealed that stimulation of mitochondrial biogenesis by carvedilol resulted in functional gain of the mitochondria by showing increased oxygen consumption and mitochondrial respiratory rate. The increased expression of PGC-1α and mitochondrial biogenesis induced by Carvedilol might suggest a new mechanism of the therapeutic effects of Carvedilol in heart failure.

Copyright © 2016. Published by Elsevier Inc. Keywords: Carvedilol, Endothelium, Mitochondria, PGC-1α

Labels: MiParea: Respiration, mt-Biogenesis;mt-density, mtDNA;mt-genetics, Pharmacology;toxicology 

Organism: Human  Tissue;cell: Endothelial;epithelial;mesothelial cell, HUVEC  Preparation: Intact cells 

Coupling state: ROUTINE 

HRR: Oxygraph-2k