Wilburn 2024 Sci Rep
| Wilburn D, Miserlis D, Fletcher E, Papoutsi E, Ismaeel A, Bradley C, Ring A, Wilkinson T, Smith RS, Ferrer L, Haynatzki G, Monteleone P, Banerjee S, Brisbois E, Bohannon WT, Koutakis P (2024) Skeletal muscle desmin alterations following revascularization in peripheral artery disease claudicants. Sci Rep 14:12609. https://doi.org/10.1038/s41598-024-63626-3 |
Wilburn Dylan, Miserlis Dimitrios, Fletcher Emma, Papoutsi Evlampia, Ismaeel Ahmed, Bradley Cassandra, Ring Andrew, Wilkinson Trevor, Smith Robert S, Ferrer Lucas, Haynatzki Gleb, Monteleone Peter, Banerjee Subhash, Brisbois Elizabeth, Bohannon William T, Koutakis Panagiotis (2024) Sci Rep
Abstract: Peripheral artery disease (PAD) is characterized by varying severity of arterial stenosis, exercise induced claudication, malperfused tissue precluding normal healing and skeletal muscle dysfunction. Revascularization interventions improve circulation, but post-reperfusion changes within the skeletal muscle are not well characterized. This study investigates if revascularization enhanced hemodynamics increases walking performance with concurrent improvement of mitochondrial function and reverses abnormal skeletal muscle morphological features that develop with PAD. Fifty-eight patients completed walking performance testing and muscle biopsy before and 6 months after revascularization procedures. Muscle fiber morphology, desmin structure, and mitochondria respiration assessments before and after the revascularization were evaluated. Revascularization improved limb hemodynamics, walking function, and muscle morphology. Qualitatively not all participants recovered normal structural architecture of desmin in the myopathic myofibers after revascularization. Heterogenous responses in the recovery of desmin structure following revascularization may be caused by other underlying factors not reversed with hemodynamic improvements. Revascularization interventions clinically improve patient walking ability and can reverse the multiple subcellular functional and structural abnormalities in muscle cells. Further study is needed to characterize desmin structural remodeling with improvements in skeletal muscle morphology and function.
• Bioblast editor: Plangger M • O2k-Network Lab: US TX Waco Koutakis P
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style, Patients
Pathology: Cardiovascular
Stress:Ischemia-reperfusion
Organism: Human
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS
Pathway: N, S, DQ, CIV, NS, ROX
HRR: Oxygraph-2k
2024-07
