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Wiedemann 1998 FEBS Lett

From Bioblast
Publications in the MiPMap
Wiedemann FR, Kunz WS (1998) Oxygen dependence of flux control of cytochrome c oxidase โ€“ implications for mitochondrial diseases. FEBS Lett 422:33-5.

ยป PMID: 9475164 Open Access

Wiedemann FR, Kunz WS (1998) FEBS Lett

Abstract: The oxygen dependence of cytochrome c oxidase control on succinate oxidation was investigated in saponin-permeabilized muscle fibers and isolated mitochondria from mouse quadriceps muscle applying metabolic control analysis. For this cyanide titrations of the oxygen consumption in the presence of succinate+rotenone were performed at different oxygen concentrations in the medium. While with isolated mitochondria high flux control coefficients were detected only at oxygen concentrations close to the KM value of cytochrome c oxidase, with saponin-permeabilized fibers a significant increase of cytochrome c oxidase flux control was already observed below 130 ฮผM oxygen. The result is in line with the high oxygen sensitivity of maximal respiration of saponin-permeabilized muscle fibers (P50=33 ฮผM) caused most probably by oxygen diffusion gradients through the fiber lattice. The oxygen dependence of cytochrome c oxidase flux control in muscle fibers can explain the pathological phenotype of mild cytochrome c oxidase deficiencies in mitochondrial myopathies. โ€ข Keywords: Oxygen, Cytochrome c oxidase, Metabolic control analysis, Mitochondrion, Saponin-permeabilized fiber, Mitochondrial disease


Labels: MiParea: Respiration 


Organism: Mouse  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue, Isolated mitochondria  Enzyme: Complex IV;cytochrome c oxidase  Regulation: ADP, Oxygen kinetics, Substrate  Coupling state: OXPHOS 

HRR: Oxygraph-2k