O2k-Virtual Support self-study material

                  

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O2k-Virtual Support self-study material

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MitoPedia O2k and high-resolution respirometry: O2k-Open Support 

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General information

To benefit optimally from the tutored training sessions of the O2k-Virtual Support, please make yourself familiar with Materials for self-study in advance.

O2k-Basic

Part 1.1: OroboPOS service and O2k instrumental

• O2k-Manual: O2k-FluoRespirometer
• O2k-Manual: POS Service
• Step 1: O2k-Videosupport: OroboPOS from Perspex housing
• Step 2: O2k-Videosupport: Cathode cleaning
• Step 3: O2k-Videosupport: Anode cleaning
• Step 4: O2k-Videosupport: Membrane mounting
• Step 5: O2k-Videosupport: DL7 Start DatLab7
• Step 6: O2k-Videosupport: DL7 Insert OroboPOS
• Step 7: O2k-Videosupport: Insert O2k Chamber
• Step 8: O2k-Videosupport: O2k Chamber volume calibration

Part 1.2: DatLab and O₂ calibration

• MitoPedia: DatLab
• O2k-Manual: DatLab 7 Guide
• DL-Protocols
• Gnaiger 2008 POS
• SOP: O2k-cleaning and ISS
• SOP: O₂-calibration
• O2k-Videosupport: QC1: POS calibration

Part 1.3: O₂ instrumental background

• SOP: O₂ background

Part 2.1: Biological samples and experimental design

Pathway control and coupling control of mitochondrial respiration:

• BEC 2020.2 Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis
• BEC 2020.1 Mitochondrial Physiology

Sample types,mitochondrial preparations and sample addition:

• Sample
• Mitochondrial preparations
• Shredder vs Fibers
• O2k-Videosupport: Shredder tissue homogenate
• O2k-Videosupport: Fiber permeabilization
• Cell count and normalization in HRR
• O2k-Videosupport: Addition of biological sample to the respirometer chamber

SUIT protocols, SUITbrowser, DL-Protocols:

• MitoPedia: SUIT
• Oroboros SUITbrowser
• O2k-Videosupport: How to find a DL-Protocol (DLP)
• O2k-Videosupport: Export DL-Protocol User (*.DLPU)

Part 2.2: Biological experiment and data analysis

• SOP: Hamilton microsyringes
• O2k-Titrations
• O2k-Videosupport: How to perform an experiment with a SUIT DL-Protocol (DLP)
• Oxygen flux analysis
• O2k-Videosupport: O₂ flux analysis with DatLab 7.4

O2k-Advanced

O2k-Applications: Simultaneous determination of O₂ and H₂O₂ fluxes

• Amplex UltraRed
• O2k-Videosupport: Smart Fluo-Sensor assembly
• O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor
• Calibration with H₂O₂
• H₂O₂ flux analysis
• O2k-Videosupport: H₂O₂ flux analysis with DatLab 7.4