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Template:SUIT-031 Q ce-pce D074

From Bioblast
MitoPedia: SUIT

Steps and respiratory states

Ce1;1Dig;1Q2;1PM;2D;3S;4Rot;5U;6Anox;7Ama.png

Step State Pathway Q-junction Comment - Events (E) and Marks (M)
ce1 ROUTINE ce1
  • ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state.
1Dig REN ce1;1Dig
  • Optimum effective digitonin concentration for complete plasma membrane permeabilization.
Step State Pathway Q-junction Comment - Events (E) and Marks (M)
1Q2 1Q2
  • To detect the redox state of the Q-pool a mimetic coenzyme Q2 is applied in suspension, which reacts both with the detecting electrode and the biological sites (CI, CII and CIII).
1PM PML(n) N CI 1Q2;1PM
2D PMP N CI 1Q2;1PM;2D
3S PMSP NS CI&II 1Q2;1PM;2D;3S
  • Respiratory stimulation by simultaneous action of type N substrates & succinate, with convergent electron flow in the NS-pathway for reconstitution of TCA cycle function.
  • OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
4Rot SP S CII 1Q2;1PM;2D;3S;4Rot
5U SE N CII 1Q2;1PM;2D;3S;4Rot;5U
6Anox 1Q2;1PM;2D;3S;4Rot;5U;6Anox
  • Anoxia is a crucial step to detect the fully reduced Q-junction in the presence of coenzyme Q2 after the biological sample has consumed the O2 in the O2k-chamber for calculation of the Q redox state.
7Ama ROX 1Q2;1PM;2D;3S;4Rot;5U;6Anox;7Ama
  • Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).



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