- high-resolution terminology - matching measurements at high-resolution
Talk:Permeabilization of plasma membrane
Description
Selective permeabilization of plasma membrane or isolation of mitochondria are a requisite for OXPHOS analysis since several mitochondrial substrates (e.g., ADP, cytochrome c) are not permeable to the plasma membrane. Plasma membrane permeabilization with effective wash-out of free cytosolic molecules including adenylates, substrates, and cytosolic enzymes makes externally added compounds accessible to the mitochondria. Plasma membrane can be permeabilized mechanically (e.g., liver tissue) and chemically by mild detergents (i.e., digitonin or saponin). See also: Permeabilized muscle fibers, Permeabilized cells, Permeabilized tissue, Permeabilized tissue or cells.
- » MitoPedia topic: Mitochondrial preparations
Reference: Doerrier 2018 Methods Mol Biol
MitoPedia methods: Respirometry
MitoPedia topics: Sample preparation
MitoPedia: permeabilization of plasma membrane
- Permeabilized tissue, pti
- Permeabilized muscle fibers, pfi
- Permeabilized cells, pce
References
| Bioblast link | Reference | Year |
|---|---|---|
| BEC 2020.1 doi10.26124bec2020-0001.v1 | Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1 | 2020 |
Labels:
Preparation: Permeabilized tissue, Permeabilized cells, Permeabilized muscle fibers
