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Least squares method + (This method makes use of all of the data p … This method makes use of all of the data points of the spectrum in order to quantify a measured spectrum with a reference spectrum of known concentration using a '''least squares method''' to match the measured spectrum with the reference spectrum. The technique results in improved accuracy compared with the use of only a few characteristic wavelengths. of only a few characteristic wavelengths.)
Paywall journalism + (Though often defined from the individual r … Though often defined from the individual reader's perspective, a paywall can also apply to an institution (such as a library) or the author. '''Paywall journalism''' is the opposite of [[Open Access]]. [[Open Science]] does not accept paywalls with the argument, that the public pays for governmentally funded research, hence research funded by public grants should be published with open access for the public without paywalls. Paywalls are most frequently defined from the perspective of the individual reader, who has to pay for an article or pay a journal subscription as a requisite for obtaining full access to the information that is otherwise hidden behind the paywall ('''reader-paywall journal'''). From the perspective of the authors, however, an '''author-paywall journal''' is defined as any journal which requests publication charges or page charges from the authors for publishing the manuscript Open Access or publishing it at all. Similarly, an '''institutional-paywall journal''' charges an institution – typically university libraries – for granting open access to the members of this institution. As long as paywall journalism prevails in science, at least '''paywall transparence''' should be required, to declare for each publication not only the reader-paywall costs but provide the full information on the author-paywall and institutional-paywall expenses.aywall and institutional-paywall expenses.)
O2k signals and output + (Three electronic '''channel types''' are a … Three electronic '''channel types''' are available in the [[O2k-MultiSensor |O2k-MultiSensor system]]. All channels are available twofold (dual-data), for O2k-Chambers A (left) and B (right), based on numerical signals sent at a fixed data sampling time interval (default: 2 s; range 0.2 s to >10 s).rval (default: 2 s; range 0.2 s to >10 s).)
Stirrer A on/off + (Toggles between stirrer on/off in the left O2k-chamber, returning to the pre set stirrer speed.)
Stirrer B on/off + (Toggles between stirrer on/off in the right O2k-chamber, returning to the pre set stirrer speed.)
Triethyltin bromide + (Triethyltin bromide (TET) is a lipophilic [1] inhibitor of the mitochondrial [[ATP synthase]] [2] which is used to induce [[LEAK state]] in [[living cells]] of ''Saccharomyces cerevisiae''.)
POS calibration - static + (Two-point calibration of the polarographic oxygen sensor, comprising [[Air calibration]] and [[Zero calibration]]. See also [[POS calibration - dynamic]].)
Unspecific binding of TPP+ + (Unspecific binding of the probe molecule T … Unspecific binding of the probe molecule TPP+ in the matrix phase of mitochondria is taken into account as a correction for measurement of the [[mitochondrial membrane potential]]. External unspecific binding is the binding outside of the inner mt-membrane or on the outer side of the inner mt-membrane, in contrast to internal unspecific binding.-membrane, in contrast to internal unspecific binding.)
SUITbrowser + (Use the '''SUITbrowser''' to find the subs … Use the '''SUITbrowser''' to find the substrate-uncoupler-inhibitor-titration ([[SUIT]]) protocol most suitable for addressing your research questions. <big><big>Open the SUITbrowser: http://suitbrowser.oroboros.at/</big></big>[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=8T33sp9KkJk]] [https://www.youtube.com/watch?v=8T33sp9KkJk How to find a DL-Protocol (DLP)]w.youtube.com/watch?v=8T33sp9KkJk How to find a DL-Protocol (DLP)])
Getting started - DatLab + (Users have to enter their user details the first time they use DatLab 8 on a specific computer. As well, entering some basic settings is required when connecting DatLab 8 with an O2k for the first time.)
Valinomycin + (Valinomycin catalyzes electrogenic K+ transport down the electrochemical transmembrane gradient (150 ng.mg-1 protein).)
Smoothing + (Various methods of '''smoothing''' can be … Various methods of '''smoothing''' can be applied to improve the [[signal-to-noise ratio]]. For instance, data points recorded over time [s] or over a range of wavelengths [nm] can be smoothed by averaging ''n'' data points per interval. Then the average of the ''n'' points per smoothing interval can be taken for each successively recorded data point across the time range or range of the spectrum to give a ''n''-point moving average smoothing. This method decreases the [[noise]] of the signal, but clearly reduces the time or wavelength [[resolution]]. More advanced methods of smoothing are applied to retain a higher [[time resolution]] or wavelength resolution.[[time resolution]] or wavelength resolution.)
Hydrogenion flux + (Volume-specific '''hydrogenion flux''' or … Volume-specific '''hydrogenion flux''' or H+ flux is measured in a closed system as the time derivative of H+ concentration, expressed in units [pmol·s-1·mL-1]. H+ flux can be measured in an open system at steady state, when any acidification of the medium is compensated by external supply of an equivalent amount of base. The extracellular acidification rate (ECAR) is the change of pH in the incubation medium over time, which is zero at steady state. Volume-specific H+ flux is comparable to volume-specific [[oxygen flux]] [pmol·s-1·mL-1], which is the (negative) time derivative of oxygen concentration measured in a closed system, corrected for instrumental and chemical background.[[pH]] is the negative logarithm of hydrogen ion activity. Therefore, ECAR is of interest in relation to acidification issues in the incubation buffer or culture medium. The physiologically relevant metabolic H+ flux, however, must not be confused with ECAR.e incubation buffer or culture medium. The physiologically relevant metabolic H+ flux, however, must not be confused with ECAR.)
Different O2 fluxes in left and right chamber + (What are potential causes for '''different O2 fluxes in the left and right chamber'''?)
Transmittance + (When light enter a sample, '''transmittance''' (''T'') is the fraction of the intensity (''I'') of the light emerging from the sample compared with the incident light intensity (''I''''0''): ''T'' = ''I''/''I''''0''.)
Absorption + (When light enters a sample and emerges wit … When light enters a sample and emerges with an intensity (''I''), '''absorption''' (''Abs'') is the fraction of the light absorbed by the sample compared with the [[incident light]] intensity (''I''''0''): ''Abs'' = 1-''I''/''I''''0''. Absorption can also be expressed as ''Abs'' = 1-''T'', where ''T'' is the [[transmittance]].[[transmittance]].)
Absorbance spectrum + (When light enters a sample, the amount of … When light enters a sample, the amount of light that it absorbs is dependent upon the wavelength of the incident light. The '''absorbance spectrum''' is the curve derived by plotting the measured [[absorbance]] against the wavelength of the light emerging from the sample over a given [[wavelength range]]. An [[absorbance spectrum]] may be characterised by peaks and troughs (absorbance maxima and minima) that can be used to identify, and sometimes quantify, different absorbing substances present in a sample. absorbing substances present in a sample.)
O2k-MultiSensor + (When one (or more) analytical parameters a … When one (or more) analytical parameters are monitored simultaneously with oxygen concentration and oxygen flux, this is an '''O2k-MultiSensor''' application of the [[Oroboros O2k-technology]]. The [[NextGen-O2k]] supports all O2k-MultiSensor Modules, while the O2k does not provide for the Q- and NADH-Redox-Modules. For some O2k-MultiSensor applications it is necessary to introduce one or more additional sensors into the chamber through a MultiSensor stopper. Optical applications require the standard black stoppers.tions require the standard black stoppers.)
TIP2k syringe blocked + (When the '''TIP2k syringe is blocked''', it must not be used with the TIP2k, and specific cleaning instructions should be followed.)
Living Communications + (With '''Living Communications''', [https:/ … With '''Living Communications''', [https://www.bioenergetics-communications.org/index.php/bec Bioenergetics Communications] (BEC) takes the next step from pre-print to re-print. The concept of ''Living Communications'' pursues a novel culture of scientific communication, addressing the conflict between long-term elaboration and validation of results versus sharing without delay improved methods and preliminary findings. Following the preprint concept, updates may be posted on the BEC website of the resource publication. Updated versions of Living Communications are submitted for Open Peer Review with full traceability. In contrast to static papers, evolution of ''Living Communications'' is more resourceful and efficient than a ‘new’ publication. ''Living Communications'' provide a pathway along the scientific culture of lively debate towards tested and trusted milestones of research, from pre-print to re-print, from initial steps to next steps.e-print, from initial steps to next steps.)
Internal flow + (Within the system boundaries, irreversible … Within the system boundaries, irreversible '''internal flows''', ''I''i,—including chemical reactions and the dissipation of internal gradients of heat and matter—contribute to internal entropy production, di''S''/d''t''. In contrast, [[external flow]]s, ''I''e, of heat, work, and matter proceed reversibly across the system boundaries (of zero thickness). Flows are expressed in various [[format]]s per unit of time, with corresponding [[motive unit]]s [MU], such as chemical [mol], electrical [C], mass [kg]. Flow is an [[extensive quantity]], in contrast to [[flux]] as a [[specific quantity]].ecific quantity]].)
Liver mitochondria purification + ([[Armstrong 2010 J Comp Physiol B]]: This paper describes a method for purification of rodent liver mitochdondria using relatively low-speed centrifugation through discontinuous Percoll gradients.)
File:MitoFitPreprints and BEC manuscript template.docx + ([[Bioenergetics Communications]] and [[MitoFit Preprints]] manuscript template.)
ET-pathway competent state + ([[Electron transfer pathway]] competent state, ''see'' '''[[Electron-transfer-pathway state]]'''.)
Duroquinol + ([[Electron-transfer-pathway state |ET-path … [[Electron-transfer-pathway state |ET-pathway level 2]] is supported by '''duroquinol''' DQ feeding electrons into Complex III (CIII) with further electron transfer to CIV and oxygen. Upstream pathways are inhibited by rotenone and malonic acid in the absence of other substrates linked to ET-pathways with entry into the Q-junction.T-pathways with entry into the Q-junction.)

Fluorometric dyes + ([[Extrinsic fluorophores]]; fluorescent markers.)

SUIT-014 + ([[File: 1GM;2D;3P;4S;5U;6Rot-.png|400px]])
O2k-sV-Module + ([[File:11200-01.jpg|180px|right]] The ''' … [[File:11200-01.jpg|180px|right]] The '''O2k-sV-Module''' is the O2k small-volume module, comprised of two Duran® glass chambers of 12 mm inner diameter specifically developed to perform high-resolution respirometry with reduced amounts of biological sample, and all the components necessary for a smaller operation volume ''V'' of 0.5 mL. The current DatLab version is included in the delivery of this revolutionary module.the delivery of this revolutionary module.)
SUIT-033 + ([[File:1D.1;2PGM;3D2.5-.png|450px]])
SUIT-038 O2 mt D091 + ([[File:1D;2M.1;2H2O;2c;3M.2;3M.5;3M1;3M2;4P;5G;6S10;6S50;7Gp;8U;9Rot;10Ama.png|400px]])
SUIT-041 O2 mt D096 + ([[File:1D;2M.1;3AC;3c;4M2;5P;6S;7Rot;8Ama.png|400px]])
SUIT-037 O2 mt D090 + ([[File:1D;2M.1;3Oct;3c;4M.2;4M.5;4M1;4M2;5P;6G;7S10;7S50;8Gp;9U;10Rot;11Ama.png|400px]])
SUIT-002 O2 mt D005 + ([[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd.png|400px]])
SUIT-025 + ([[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Rot-.png|600px]])
SUIT-025 O2 mt D057 + ([[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Rot;9Ama.png|600px]])
SUIT-002 + ([[File:1D;2M.1;3Oct;4M2;5P;6G;7S;8Gp;9U;10Rot-.png|400px]])
SUIT-036 O2 mt D089 + ([[File:1D;2M.1;3Pal;3c;4M.2;4M.5;4M1;4M2;5P;6G;7S10;7S50;8Gp;9U;10Rot;11Ama.png|400px]])
SUIT-040 O2 mt D094 + ([[File:1D;2M.1;3Pal;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama.png|400px]])
SUIT-040 O2 pfi D095 + ([[File:1D;2M.1;3Pal;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama.png|400px]])
SUIT-039 O2 mt D092 + ([[File:1D;2M.1;3Pal;3c;4M2;5P;6G;7S;8U;9Rot;10Ama.png|400px]])
SUIT-039 O2 pfi D093 + ([[File:1D;2M.1;3Pal;3c;4M2;5P;6G;7S;8U;9Rot;10Ama.png|400px]])
SUIT-007 + ([[File:1G;2D;3M;4U-.png|300px]])
SUIT-014 O2 pfi D042 + ([[File:1GM;2D;2c;3P;4S;5U;6Rot;7Ama.png|400px]])
SUIT-021 O2 mt D035 + ([[File:1GM;2D;2c;3S;4Rot;5Omy;6U;7Ama.png|300px]])
SUIT-011 O2 pfi D024 + ([[File:1GM;2D;2c;3S;4U;5Rot;6Ama.png|400px]])
SUIT-021 + ([[File:1GM;2D;3S;4Rot;5Omy;6U-.png|400px]])
SUIT-021 Fluo mt D036 + ([[File:1GM;2D;3S;4Rot;5Omy;6U;7Ama.png|300px]])
SUIT-011 + ([[File:1GM;2D;3S;4U;5Rot-.png|400px|SUIT-011]])
SUIT-018 + ([[File:1GMS;2D-.png|300px|SUIT-018]])
SUIT-018 O2 mt D054 + ([[File:1GMS;2D;2c;3Ama.png|290px]])
SUIT-018 AmR mt D031 + ([[File:1GMS;2D;3Ama.png|290px]])