Sobotka 2024 EBEC
 |
Link: EBEC 2024 Conference website
Sobotka Ondrej, Cardoso Luiza HD, Garcia-Souza Luiz F, Sobotka Lubos, Gnaiger Erich (2024)
Event: EBEC2024_Innsbruck_AT
Introduction: Mitochondrial function plays a crucial role in cellular metabolism, with alterations observed in various diseases, including several types of cancer. The study of mitochondrial respiration in human cells provides relevant insights into metabolic diseases and potential therapeutic targets. Our study aims to evaluate the effect of glucose concentration on culture media on cell respiration in hepatocellular carcinoma (HuH-7) cells. Methods: Using high-resolution respirometry, we analyzed mitochondrial respiration in the living HuH-7 cells cultured under low glucose (LG, 5.5 mM) and high glucose (HG, 25 mM) conditions. A coupling control protocol assessed ROUTINE respiration, LEAK respiration, electron transfer (ET) capacity, and E-L coupling efficiency. The protocol was extended to estimate cell viability in terms of plasma membrane permeability. Results: A decrease was observed in ROUTINE respiration in the HG group compared to the LG group, with no difference in ET-capacity and coupling efficiency. A notable Crabtree effect, indicative of metabolic reprogramming towards glycolysis, was observed exclusively in the LG group. Discussion: These findings suggest that high glucose concentrations in culture media can induce metabolic reprogramming in hepatocellular carcinoma cells, affecting mitochondrial function. Further investigations into aerobic glycolysis under varying glucose conditions are warranted to expand upon these respirometric findings. Our study underscores the importance of considering culture conditions in mitochondrial research and functional diagnostics.
• Bioblast editor: Sobotka O
• O2k-Network Lab: AT Innsbruck Oroboros, AT Innsbruck MitoFit
Download as PDF here: File:2024 EBEC Sobotka O Poster.pdf
Listen to audio presentation here:
Labels: MiParea: Respiration
Organism: Human
Preparation: Permeabilized cells, Intact cells
Coupling state: ET, LEAK, ROUTINE, OXPHOS
Pathway: S, NS
HRR: Oxygraph-2k
Affiliations
- Ondrej Sobotka1,2, Luiza Cardoso1, Luiz Garcia-Souza1,3, Lubos Sobotka2, Erich Gnaiger1
- [email protected]
- Oroboros Instruments, Innsbruck, Austria
- 3rd Department of Internal Medicine, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic
- Institute of Pathophysiology, Medical University of Innsbruck, Austria
References
- Sobotka L, Sobotka O (2021). The predominant role of glucose as a building block and precursor of reducing equivalents. Curr Opin Clin Nutr Metab Care 24:555–62.
- Soeters PB, Shenkin A, Sobotka L, Soeters MR, de Leeuw PW, Wolfe RR (2021) The anabolic role of the Warburg, Cori-cycle and Crabtree effects in health and disease. Clin Nutr 40:2988-98.
- Gnaiger E, Kemp RB (1990) Anaerobic metabolism in aerobic mammalian cells: information from the ratio of calorimetric heat flux and respirometric oxygen flux. Biochim Biophys Acta 1016:328-32.
- Stoner HB, Little RA, Frayn KN, Elebute AE, Tresadern J, Gross E (1983) The effect of sepsis on the oxidation of carbohydrate and fat. Br J Surg 70:32-5.
