Sjoevall 2013 Mitochondrion

From Bioblast
Publications in the MiPMap
SjΓΆvall F, Ehinger JK, Marelsson SE, Morota S, Asander Frostner E, Uchino H, Lundgren J, ArnbjΓΆrnsson E, Hansson Magnus J, Fellman V, ElmΓ©r E (2013) Mitochondrial respiration in human viable platelets - methodology and influence of gender, age and storage. Mitochondrion 13:7-14.

Β» PMID: 23164798 Open Access

Sjoevall F, Ehinger JK, Marelsson SE, Morota S, Asander Frostner E, Uchino H, Lundgren J, Arnbjoernsson E, Hansson Magnus J, Fellman V, Elmer E (2013) Mitochondrion

Abstract: Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability. β€’ Keywords: Diagnostics; Human; Platelets; Mitochondria; OXPHOS; High-resolution respirometry; Mitochondrial disorders

β€’ O2k-Network Lab: SE Lund Elmer E, JP Tokyo Uchino H

Labels: MiParea: Respiration, Instruments;methods, mt-Medicine, Patients  Pathology: Aging;senescence, Neurodegenerative  Stress:Ischemia-reperfusion  Organism: Human  Tissue;cell: Blood cells, Platelet  Preparation: Permeabilized cells, Intact cells 

Coupling state: LEAK, ROUTINE, OXPHOS, ET  Pathway: N, ROX  HRR: Oxygraph-2k 

SE, JP, MitoEAGLE blood cells data 



Cookies help us deliver our services. By using our services, you agree to our use of cookies.