Shiva 2007 Methods Cell Bio
| Shiva S, Brookes PS, Darley-Usmar VM (2007) Methods for measuring the regulation of respiration by nitric oxide. Methods Cell Biol 80:395-416. |
Shiva S, Brookes PS, Darley-Usmar VM (2007) Methods Cell Biol
Abstract: This chapter outlines the basic methodologies for the measurement of NO· dependent effects on mitochondrial respiration, including the measurement of respiratory thresholds in isolated mitochondria and cell systems. The methods described in the chapter serve to illustrate not only the importance of measuring NO· thresholds in mitochondria and cells but also highlight many of the considerations that must be taken when making such measurements. These include the presence of O2, the source of NO·, the precise design of the apparatus, and experimental conditions such as buffer composition, light level, and temperature. When all these factors are considered, accurate interpretations of the role of NO· in controlling mitochondrial function can be made. Such experiments can provide insight into intricate nature of the interaction between NO· and mitochondria and the variations in this system, which can occur under both physiological and pathological conditions. The chapter also describes the methodology used to measure respiration at low O2 concentrations and the complications of using various NO· donors. It also discusses the use of these methodologies to measure mitochondrial function in physiology and the change in function observed in pathology. • Keywords: Animals, Calibration, Cell Hypoxia, Cell Respiration, Electrodes, Humans, Mitochondria, Nitric Oxide, Oxygen, Oxygen Consumption and Reperfusion Injury
Labels: MiParea: Respiration
