Palmitoylcarnitine

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Palmitoylcarnitine

Description

Palmitoylcarnitine is an ester derivative of carnitine (long-chain acylcarnitine) involved in the metabolism of fatty acids. Within the cell, palmitoylcarnitine is transported into the mitochondria to deliver palmitate for fatty acid oxidation and energy production.

Abbreviation: Pal

Reference: Cecatto 2023 MitoFit


Carnitine palmitoyltransferase I (CPT1) also known as carnitine acyltransferase I or CAT1 is a mitochondrial enzyme. CPT1 is associated with the outer mitochondrial membrane and mediates the transport of long-chain fatty acids across the membrane by binding them to carnitine.


Application in HRR

Pal: Palmitoylcarnitine (Palmitoyl-DL-carnitine-HCl; C23H46NO4 Β· Cl), Sigma-Aldrich: P4509, store at -20 Β°C, CAS: 6865-14-1, M = 436.07 gΒ·mol-1


Preparation of 10 mM stock solution (dissolved in H2O):
  1. Weigh 8.72 mg of palmitoylcarnitine.
  2. Dissolve in 2 mL H2O.
  3. Divide into 0.2 mL portions.
  4. Store frozen at -20 Β°C.
Β» O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume: 2βˆ’8 Β΅L using a 10 Β΅L syringe (2 mL O2k-chamber).
  • Final concentration: 10βˆ’40 Β΅M. We suggest testing for the optimum concentration for your sample, as palmitoylcarnitine may inhibit respiration (Cecatto 2023 MitoFit).







MitoPedia topics: Substrate and metabolite 


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MitoPedia:FAT4BRAIN 

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