Osiki 2016 Abstract MitoFit Science Camp 2016
Suitability of octanoylcarnitine & malate for assessment of beta oxidation capacity by respirometry in aconitase-inhibited samples. |
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Event: MitoFit Science Camp 2016 Kuehtai AT
The capacity of cells, tissues or isolated mitochondria to oxidize fatty acids is often measured by respirometry using acyl carnitines & malate as substrates [1]. Inclusion of malate as a co-substrate is essential to prevent feed forward inhibition of beta oxidation arising from matrix CoA depletion as a result of short-chain acyl-CoA accumulation [2]. Malate is dehydrogenated to oxaloacetate which condenses with the beta-oxidation-derived acetyl-CoA to form citrate and CoA. Therefore, as long as citrate does not accumulate in the mitochondrial matrix and inhibit the above series of reactions, beta oxidation reactions are likely to keep running. However, a number of disease conditions are known to inhibit mitochondrial aconitase, a mitochondrial enzyme that has profound effects on citrate metabolism [3,4].
The purpose of this study was therefore to investigate the validity of beta-oxidation assessment by respirometry using octanoylcarnitine & malate in rat soleus muscle when mitochondrial aconitase is chemically inhibited by 5 mM Oxalomalic acid. Our results indicate that inhibition of aconitase by 25% does not decrease oxygen flux at all respiratory states but increased citrate levels in the mitochondrial media by 40% compared to controls. However, as expected, oxygen fluxes were significantly diminished at OXPHOS and ET-pathway respiratory states by ~50% when medium chain acyl dehydrogenase, a rate limiting beta-oxidation enzyme, was inhibited by 1 mm 2-mercaptoacetate.
Taken together, our data indicate that in respirometric assays, use of octanoylcarnitine + malate allows for a valid assessment of beta-oxidation capacity in skeletal muscle under conditions where mitochondrial aconitase activity is compromised.
β’ O2k-Network Lab: ZA Cape Town Smith J, ZA Cape Town Ojuka EO
Labels: MiParea: Respiration
Organism: Rat
Tissue;cell: Skeletal muscle
Regulation: Substrate
Coupling state: OXPHOS, ET
Pathway: F, N, Other combinations
HRR: Oxygraph-2k
Event: A1
MitoFit Science Camp 2016
Affiliations
Dept Human Biol, Univ Cape Town, South Africa. - [email protected]
References
- Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. Oroboros MiPNet Publications, Innsbruck:80 pp.
- Van Eunen K, Simons SM, Gerding A, Bleeker A, den Besten G, Touw CM, Houten SM, Groen BK, Krab K, Reijngoud DJ, Bakker BM (2013) Biochemical competition makes fatty-acid Ξ²-oxidation vulnerable to substrate overload. PLoS Comput Biol 9:e1003186.
- Zhang D, Liu ZX, Choi CS, Tian L, Kibbey R, Dong J, Cline GW, Wood PA, Shulman GI (2007) Mitochondrial dysfunction due to long-chain Acyl-CoA dehydrogenase deficiency causes hepatic steatosis and hepatic insulin resistance. Proc Natl Acad Sci USA 104: 17075-80.
- Warren BE, Lou PH, Lucchinetti E, Zhang L, Clanachan AS, Affolter A, Hersberger M, Zaugg M, Lemieux H (2014) Early mitochondrial dysfunction in glycolytic muscle, but not oxidative muscle, of the fructose-fed insulin-resistant rat. Am J Physiol Endocrinol Metab 306: E658-66.