Oroboros Manual

Oroboros Manual

Study the Oroboros Manual carefully before using the Oroboros  »  User information

The Oroboros Manual comprises all information communicated in separate files (Chapters) and several MitoPedia pages. The Oroboros Manual helps beginners and experienced users. Context-sensitive links are provided in the DatLab software.

» Oroboros NextGen-O2k • Oroboros O2k • Oroboros sole source

Current Oroboros packages » NextGen-O2k Series XB and O2k Series J
Current DatLab version » DatLab 8.2

OroM1: Oroboros start

Chapter	View	Topic	Last update
OroM.A User information	» Versions	Oroboros Manual: user information. Oroboros Manual OroM.A(v11) - PLEASE STUDY THIS MANUAL.	2025-01-12
OroM.B Oroboros specifications	» Versions	Oroboros instrument specifications for high-resolution respirometry and precision OXPHOS analysis. Oroboros Manual OroM.B(v12)	2025-01-14
OroM1.1 Oroboros Startup	» Versions	Oroboros Startup. Oroboros Manual OroM1.1(v1) - in preparation	2025-01-11
OroM1.2 POS service	» Versions	Service of the polarographic oxygen sensor POS. Oroboros Manual OroM1.2(v24)	2025-01-12
OroM1.3 Chamber assembly	» Versions	Oroboros chamber assembly and volume calibration. Oroboros Manual OroM1.3(v4) - in preparation	2025-01-12
OroM1.4 ISS and chamber cleaning	» Versions	Integrated Suction System ISS and chamber cleaning. Oroboros Manual OroM1.4(9) - in preparation	2023-03-14
OroM1.5 Oroboros checklist	» Versions	Oroboros checklist: get started with high-resolution respirometry. Oroboros Manual OroM1.5(06) - in preparation	2021-03-12

NextGen-O2k Manuals

Chapter	View	Section	Last update
MiPNet26.13 NextGen-O2k Series XA Manual	» Versions	NextGen-O2k Series XA Manual.	2022-07-25
MiPNet28.07 NextGen-O2k Series XB Manual	» Versions	NextGen-O2k Series XB Manual.	2022-02-20

O2k Manuals: Series H, I, J

Chapter	View	Section	Last update
MiPNet22.11 O2k-FluoRespirometer manual	» Versions	Oroboros O2k-Series H and I Manual.	2023-03-14
MiPNet28.08 Oroboros O2k Series J Manual	» Versions	Oroboros O2k-Series J Manual.	2024-02-20

OroM2: Chemicals and titrations

Chapter	View	Topic	Last update
MiPNet09.12 Manual titrations of SUIT chemicals	»Versions	Manual titrations of SUIT chemicals with 2.0 mL and 0.5 mL chambers of the Oroboros Bioenergetics Analyzer. Mitochondr Physiol Network 09.12(21)	2024-11-25
MiPNet12.10 TIP2k Manual	»Versions	Titration-Injection microPump TIP2k Manual. Mitochondr Physiol Network 12.10(09)	2021-07-08
MiPNet22.10 MiR05-Kit	» Versions	Preparation of mitochondrial respiration media with MiR05-Kit. Mitochondr Physiol Network 22.10(5).	2024-12-12
MiPNet26.14 Oxia	» Versions	Oxia - HyperOxia to HypOxia. Mitochondr Physiol Network 26.14(02)	2022-10-31
OroM2.4 Microsyringes	» Versions	Hamilton microsyringes: titrations and maintenance. Oroboros Manual OroM2.4(9) - in preparation	2024-12-10

OroM3: HRR calibrations

Chapter	View	Topic	Last update
MiPNet06.03 POS-calibration-SOP	» Versions	O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. Mitochondr Physiol Network 06.03(21)	2023-02-06
MiPNet06.06 Chemical O2 background	» Versions	Oxygraph assay of cytochrome c oxidase activity: chemical background correction. Mitochondr Physiol Network 06.06(07)	2015-04-27
MiPNet14.06 Instrumental O2 background	» Versions	O2k Quality Control 2: Instrumental oxygen background correction and accuracy of oxygen flux. Mitochondr Physiol Network 14.6(09)	2023-10-19

OroM4: Fluo

Chapter	View	Topic	Last update
MiPNet17.05 Fluo LED2-Module	»Versions	Fluo LED2-Module. Mitochondr Physiol Network 17.05(10)	2021-06-22
MiPNet24.08 Safranin Analysis Template	» Versions	Excel template for safranin data analysis. Mitochondr Physiol Network 24.08(02)	2020-05-13
MiPNet24.09 Data analysis of mt-membrane potential	» Versions	Data analysis of mitochondrial membrane potential estimation using various fluorescence dyes. Mitochondr Physiol Network 24.09(03)	2021-09-16
MiPNet24.10 H2O2 flux analysis	»Versions	Hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4. Mitochondr Physiol Network 24.10(03)	2021-10-22
MiPNet26.10 MgG data analysis	»Versions	Measurement of mitochondrial ATP production using Magnesium Green: DL-Protocols and data analysis with DatLab 7.4. Mitochondr Physiol Network 26.10(01)	2021-09-16
MiPNet28.09 Oroboros Fluo Smart-Module Manual	» Versions	Oroboros Fluo Smart-Module Manual. Mitochondr Physiol Network 28.09(01)	2023-09-25

OroM5: Ion Selective Electrodes ISE

Chapter	View	Topic	Last update
MiPNet08.16 pH calibration	» Versions	Laboratory protocol: pH measurement and temperature dependence of pH. Mitochondr Physiol Network 08.16(09)	2022-03-01
MiPNet14.05 TPP-mtMembranePotential	»Versions	TPP⁺ and mt-membrane potential. Mitochondr Physiol Network 14.05.	2016-04-01
MiPNet15.03 O2k-MultiSensor-ISE	»Versions	O2k-MultiSensor system with ion selective electrodes (ISE). Mitochondr Physiol Network 15.03(09)	2021-08-02
MiPNet15.08 TPP electrode	»Versions	Ion selective electrode for TPP⁺ and high-resolution respirometry. Mitochondr Physiol Network 15.8.	2011-12-11
MiPNet23.15 pH ISE-Module		Measurement of proton flux with the pH ISE-Module. Mitochondr Physiol Network 23.12(01)	2018-09-08
MiPNet24.11 mtMP calculation	» Versions	Mitochondrial membrane potential calculation. Mitochondr Physiol Network 24.11(01)	2019-08-05

OroM6: Redox and NO

Chapter	View	Topic	Last update
MiPNet15.05 NO Manual	»Versions	Oroboros MultiSensor system with amperometric sensors: NO, H₂S, H₂O₂. Mitochondr Physiol Network 15.05(05)	2016-04-21
MiPNet24.12 NextGen-O2k: Q-Module	» Versions	NextGen-O2k: Q-Module manual. Mitochondr Physiol Network 24.12(04)	2021-10-29
MiPNet24.16 DatLab8.0: CV-Module	»Versions	DatLab8.0:Cyclic voltammetry manual. Mitochondr Physiol Network 24.16(03)	2021-10-28
MiPNet26.12 NextGen-O2k: NADH-Module	» Versions	NextGen-O2k: NADH-Module manual. Mitochondr Physiol Network 26.12(01)	2022-05-17

OroM7: PhotoBiology

Chapter	View	Topic	Last update
MiPNet26.11 NextGen-O2k: PB-Module	» Versions	PB-Module. Mitochondr Physiol Network 26.11(00)	2021-10-29

OroM8: Auxiliary Tools

Chapter	View	Topic	Last update
MiPNet17.02 PBI-Shredder manual	» Versions	PBI-Shredder HRR-Set: preparation of tissue homogenates for diagnosis of mitochondrial respiratory function. Mitochondr Physiol Network 17.02(03)	2015-06-19
MiPNet27.07 Sample Holder	» Versions	Sample Holder. Mitochondr Physiol Network 27.07(01)	2022-08-09

Oroboros Videosupport

» Oroboros Videosupport

O2k-Series A to G; DatLab 5 and 6

Chapter	View	Section	Last update
MiPNet19.18E O2 flux analysis - DatLab 6	»Versions	Oxygen flux analysis: DatLab real-time.	2016-08-08
MiPNet19.18C DatLab 6: Guide	»Versions	DatLab-guide through the menus.	2016-08-24
MiPNet19.18A O2k-Series G: Start	» Versions	Oroboros O2k: start high-resolution respirometry.	2016-08-08
MiPNet19.18 O2k-Series G Core manual	»Versions	O2k-Core manual contents.	2016-08-08
MiPNet19.01 O2k-Core Manual Series F DatLab 5	»Versions	O2k-Core Manual - up to O2k-Series F and DatLab 5.	2014-03
MiPNet17.05 Fluo LED2-Module	»Versions	Fluo LED2-Module. Mitochondr Physiol Network 17.05(10)	2021-06-22

All OroM Chapters

	View	Reference	Published
OroM.A User information	» Versions	Oroboros Manual: user information. Oroboros Manual OroM.A(v11) - PLEASE STUDY THIS MANUAL.	2025-01-12
OroM.B Oroboros specifications	» Versions	Oroboros instrument specifications for high-resolution respirometry and precision OXPHOS analysis. Oroboros Manual OroM.B(v12)	2025-01-14
OroM1.1 Oroboros Startup	» Versions	Oroboros Startup. Oroboros Manual OroM1.1(v1) - in preparation	2025-01-11
OroM1.2 POS service	» Versions	Service of the polarographic oxygen sensor POS. Oroboros Manual OroM1.2(v24)	2025-01-12
OroM1.3 Chamber assembly	» Versions	Oroboros chamber assembly and volume calibration. Oroboros Manual OroM1.3(v4) - in preparation	2025-01-12
OroM1.4 ISS and chamber cleaning	» Versions	Integrated Suction System ISS and chamber cleaning. Oroboros Manual OroM1.4(9) - in preparation	2023-03-14
OroM1.5 Oroboros checklist	» Versions	Oroboros checklist: get started with high-resolution respirometry. Oroboros Manual OroM1.5(06) - in preparation	2021-03-12
OroM2.4 Microsyringes	» Versions	Hamilton microsyringes: titrations and maintenance. Oroboros Manual OroM2.4(9) - in preparation	2024-12-10

Orboros Manual - Chapters in chronological sequence