From Bioblast
 |
O2k-Demo experiments |
Oroboros O2k demo
| Protocol | View | Topic | Preparation | Tissue and cell | Mammal and model | Last update |
|---|---|---|---|---|---|---|
| Gnaiger 2020 BEC MitoPathways | Bioenerg Commun 2020.2.  published online 2020-12-30
| Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. https://doi.org/10.26124/bec:2020-0002 | Permeabilized cells Permeabilized tissue Homogenate Isolated mitochondria Intact cells | Heart Skeletal muscle Fibroblast | Human Mouse | 2020 |
| MiPNet12.01 Suppl T-issue |  »Versions |  | Permeabilized tissue | Skeletal muscle | Mouse | 2018-11-18 |
| MiPNet08.09 CellRespiration | »Versions | | Intact cells | Blood cells Lymphocyte | Human | 2016-08-11 |
| MiPNet17.03 Shredder vs Fibers | »Versions | | Homogenate | Heart Liver | Fishes | 2015-06-18 |
| MiPNet12.11 MitoRespiration | »Versions | | Isolated mitochondria | Artemia Crustaceans | 2014-02-20 | |
| Pesta 2012 Methods Mol Biol | PMID: 22057559
| Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_3 | Permeabilized cells Permeabilized tissue Intact cells | Skeletal muscle Other cell lines HEK Fibroblast HUVEC | Human | 2012 |
| Gnaiger 2008 POS | | Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52. | Permeabilized cells Permeabilized tissue Intact cells | Blood cells | Mouse | 2008 |
O2k-Demo TIP2k
| Protocol | View | Topic | Preparation | Tissue and cell | Mammal and model | Last update |
|---|---|---|---|---|---|---|
| Pesta 2012 Methods Mol Biol | PMID: 22057559
| Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_3 | Permeabilized cells Permeabilized tissue Intact cells | Skeletal muscle Other cell lines HEK Fibroblast HUVEC | Human | 2012 |
| Gnaiger 2008 POS | | Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52. | Permeabilized cells Permeabilized tissue Intact cells | Blood cells | Mouse | 2008 |
| MiPNet04.05 Titration-Injection | | | Intact cells | Endothelial;epithelial;mesothelial cell HUVEC | Human | 1999-01 |
TIP2k Manual
| Chapter | View | Section | Last update |
|---|---|---|---|
| MiPNet12.10 TIP2k-manual | »Versions |  | 2021-07-08 |
O2k-Demo O2k-MultiSensor
| Protocol | View | Topic | Preparation | Tissue and cell | Mammal and model | Last update |
|---|---|---|---|---|---|---|
| MiPNet24.11 mtMP calculation | » Versions | | 2019-08-05 | |||
| MiPNet20.14 AmplexRed H2O2-production | »Versions | | Isolated mitochondria | Heart | Mouse | 2019-06-24 |
| MiPNet20.13 Safranin mt-membranepotential | »Versions | | Homogenate | Nervous system | Mouse | 2019-06-24 |
| MiPNet18.05 Amplex-Mouse-heart | »Versions | | Homogenate | Heart | Mouse | 2019-06-24 |
| Krumschnabel 2015 Methods Mol Biol | PMID: 25631019 | Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. | Isolated mitochondria | Nervous system | Mouse | 2015 |
| Makrecka-Kuka 2015 Biomolecules | PMID: 26131977 Open Access | Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. https://doi.org/10.3390/biom5031319 | Permeabilized cells Homogenate Isolated mitochondria Intact cells | Heart Nervous system HEK | Human Mouse | 2015 |
| Krumschnabel 2014 Methods Enzymol | Methods Enzymol 542:163-81. PMID: 24862266 » | Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. https://doi.org/10.1016/B978-0-12-416618-9.00009-1 | Homogenate | Nervous system | Mouse | 2014 |
| MiPNet08.12 IOC22 | | | Isolated mitochondria | Liver | Rat | 2011-12-11 |
O2k-MultiSensor Manual
| Chapter | View | Section | Last update |
|---|---|---|---|
| MiPNet15.03 O2k-MultiSensor-ISE | »Versions | | 2021-08-02 |
| MiPNet15.05 NO-manual | »Versions | | 2016-04-21 |
| MiPNet17.05 O2k-Fluo LED2-Module | »Versions | | 2021-06-22 |
| MiPNet23.15 O2k-pH ISE-Module | | 2018-09-08 | |
| MiPNet24.12 NextGen-O2k: Q-Module | » Versions | | 2021-10-29 |
| MiPNet26.11 NextGen-O2k: PB-Module | » Versions | | 2021-10-29 |
| MiPNet26.12 NextGen-O2k: NADH-Module | » Versions | | 2022-05-17 |
| MiPNet26.13 NextGen-O2k manual | » Versions | | 2022-07-25 |
O2k-Guides: »O2k-Catalogue :: »O2k-Manual :: »O2k-Publications
| O2k-Procedures » Link | |
|---|---|
 |
» O2k-Procedures: MitoPathways |
 |
» O2k-Demo experiments |
|
» O2k-chemicals and media |
 |
» O2k-mitochondrial preparations |
|
» O2k-Procedures: Mitochondrial and marker-enzymes |
