Nabben 2008 FEBS Lett
| Nabben M, Hoeks J, Briedé JJ, Glatz JF, Moonen-Kornips E, Hesselink MK, Schrauwen P (2008) The effect of UCP3 overexpression on mitochondrial ROS production in skeletal muscle of young versus aged mice. FEBS Lett 582:4147-52. |
Nabben M, Hoeks J, Briede JJ, Glatz JF, Moonen-Kornips E, Hesselink MK, Schrauwen P (2008) FEBS Lett
Abstract: Uncoupling protein 3 (UCP3) is suggested to protect mitochondria against aging and lipid-induced damage, possibly via modulation of reactive oxygen species (ROS) production. Here we show that mice overexpressing UCP3 (UCP3Tg) have a blunted age-induced increase in ROS production, assessed by electron spin resonance spectroscopy, but only after addition of 4-hydroxynonenal (4-HNE). Mitochondrial function, assessed by respirometry, on glycolytic substrate was lower in UCP3Tg mice compared to wild types, whereas this tended to be higher on fatty acids. State 4o respiration was higher in UCP3Tg animals. To conclude, UCP3 overexpression leads to increased state 4o respiration and, in presence of 4-HNE, blunts the age-induced increase in ROS production. • Keywords: UCP3, Uncoupling, ROS, Lipotoxicity, Mitochondria, High resolution respirometry, 4-HNE
• O2k-Network Lab: NL Maastricht Schrauwen P
Labels: MiParea: Respiration, Genetic knockout;overexpression
Pathology: Aging;senescence, Diabetes
Stress:Oxidative stress;RONS
Organism: Mouse
Tissue;cell: Skeletal muscle
Preparation: Isolated mitochondria
Enzyme: Uncoupling protein
Regulation: ADP, Substrate, Uncoupler, Fatty acid
Coupling state: OXPHOS
HRR: Oxygraph-2k
