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McDonald 2009 FEBS Lett

From Bioblast
Publications in the MiPMap
McDonald BM, Wydrob MM, Lightowlers RN, Lakey JH (2009) Probing the orientation of yeast VDAC1 in vivo. FEBS Lett 583:739-42.

Β» PMID: 19185576 Open Access

McDonald BM, Wydrob MM, Lightowlers RN, Lakey JH (2009) FEBS Lett

Abstract: Voltage dependent anion channel (VDAC) is a vital ion channel in mitochondrial outer membranes and its structure was recently shown to be a 19 stranded beta-barrel. However the orientation of VDAC in the membrane remains unclear. We probe here the topology and membrane orientation of yeast Saccharomyces cerevisiae in vivo. Five FLAG-epitopes were independently inserted into scVDAC1 and their surface exposure in intact and disrupted mitochondria detected by immunoprecipitation. Functionality was confirmed by measurements of respiration. Two epitopes suggest that VDAC (scVDAC) has its C-terminus exposed to the cytoplasm whilst two others are more equivocal and, when combined with published data, suggest a dynamic behavior. β€’ Keywords: Mitochondrial membrane channels, Voltage dependent anion channel, Mitochondrial porin, Structure, Immunoprecipitation, Yeast

β€’ O2k-Network Lab: UK Newcastle Lightowlers RN


Labels: MiParea: Respiration, Genetic knockout;overexpression 


Organism: Saccharomyces cerevisiae, Fungi 

Preparation: Intact cells  Enzyme: Inner mt-membrane transporter 


HRR: Oxygraph-2k