Komlodi 2022 BEC

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Bioenergetics Communications        
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Gnaiger Erich et al โ€• MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1.
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Komlรณdi T, Tretter L (2022) The protonmotive force โ€“ not merely membrane potential. Bioenerg Commun 2022.16. https://doi.org/10.26124/bec:2022-0016

ยป Bioenerg Commun 2022.16. Open Access pdf
published online 2022-11-29 ยปWatch the presentationยซ

Komlodi Timea, Tretter Laszlo (2022) Bioenerg Commun

Abstract: BEC.png https://doi.org/10.26124/bec:2022-0016

The protonmotive force pmF establishes the link between electrical and chemical components of energy transformation and coupling of oxidative phosphorylation in the mitochondrial electron transfer system. The electric part corresponds to the mitochondrial membrane potential ฮ”ฮจmt and the chemical part is related to the transmembrane pH difference ฮ”pH. Although the contribution of ฮ”pH to pmF is smaller than that of ฮ”ฮจmt, ฮ”pH plays an important role in mitochondrial transport processes and regulation of reactive oxygen species production. Measurement of both ฮ”ฮจmt and ฮ”pH allows for calculation of pmF. Methods for monitoring ฮ”ฮจmt such as fluorescence dyes are generally available, while determination of ฮ”pH is more challenging. In this review, we focus on the application of the fluorescence ratiometric method using the acetoxymethyl ester form of 2,7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF/AM) for real-time monitoring of the intramitochondrial pH in isolated mitochondria. Knowing the intra- and extramitochondrial pH allows for calculating the ฮ”pH. Application of specific ionophores such as nigericin or valinomycin provides the possibility to dissect the two components of the pmF. We summarize the mitochondrial processes โ€• such as production of reactive oxygen species โ€• for which ฮ”pH plays an important role.
โ€ข Keywords: BCECF; intramitochondrial pH; matrix pH; ฮ”pH; mitochondria, mitochondrial membrane potential, ฮ”ฮจmt; nigercin; protonmotive force, pmF; reverse electron transfer, RET; safranin; triphenylphosphonium, TPP+; valinomycin โ€ข Bioblast editor: Tindle-Solomon L โ€ข O2k-Network Lab: HU Budapest Tretter L

ORCID: ORCID.png Komlodi Timea


ยป Komlodi 2022 MitoFit

Labels: MiParea: Instruments;methods, mt-Membrane 

Preparation: Isolated mitochondria  Enzyme: Complex V;ATP synthase  Regulation: ATP production, mt-Membrane potential, pH  Coupling state: LEAK, OXPHOS 

HRR: Oxygraph-2k, O2k-Fluorometer, TPP 


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