Jezek Petr

From Bioblast
Name Jezek Petr, Prof., Dr.
Petr Jezek

Institute of Physiology, Laboratory. No. 75

Mitochondrial Physiology

Address Videnska 1083, 14220
City Prague
Country Czech Republic
Email [email protected]
O2k-Network Lab CZ Prague Jezek P

Labels: Field of research: Basic 


Jezek 2023 Antioxid Redox Signal2023Ježek P, Jabůrek M, Holendová B, Engstová H, Dlasková A (2023) Mitochondrial cristae morphology reflecting metabolism, superoxide formation, redox homeostasis, and pathology. Antioxid Redox Signal 39:635–83.
Pavluch 2023 Sci Rep2023Pavluch V, Engstová H, Špačková J, Ježek P (2023) Deficiency of transcription factor Nkx6.1 does not prevent insulin secretion in INS-1E cells.
Pruuchova 2022 Antioxidants (Basel)2022Průchová P, Gotvaldová K, Smolková K, Alán L, Holendová B, Tauber J, Galkin A, Ježek P, Jabůrek M (2022) Antioxidant role and cardiolipin remodeling by redox-activated mitochondrial Ca2+-independent phospholipase A in the brain.
Leguina-Ruzzi 2020 Biomolecules2020Leguina-Ruzzi A, Vodičková A, Holendová B, Pavluch V, Tauber J, Engstová H, Dlasková A, Ježek P (2020) Glucose-induced expression of DAPIT in pancreatic β-cells. Biomolecules 10:E1026.
Plecita-Hlavata 2020 Antioxid Redox Signal2020Plecitá-Hlavatá L, Engstová H, Holendová B, Tauber J, Špaček T, Petrásková L, Křen V, Špačková J, Gotvaldová K, Ježek J, Dlasková A, Smolková K, Ježek P (2020) Mitochondrial superoxide production decreases on glucose-stimulated insulin secretion in pancreatic β cells due to decreasing mitochondrial matrix NADH/NAD+ ratio. Antioxid Redox Signal 33:789-815.
Plecita-Hlavata 2020 Diabetes2020Plecitá-Hlavatá L, Jabůrek M, Holendová B, Tauber J, Pavluch V, Berková Z, Cahová M, Schröder K, Brandes RP, Siemen D, Ježek P (2020) Glucose-stimulated insulin secretion fundamentally requires H2O2 signaling by NADPH oxidase 4 . Diabetes 69:1341-54.
Smolkova 2020 Sci Rep2020Smolková K, Špačková J, Gotvaldová K, Dvořák A, Křenková A, Hubálek M, Holendová B, Vítek L, Ježek P (2020) SIRT3 and GCN5L regulation of NADP+- and NADPH-driven reactions of mitochondrial isocitrate dehydrogenase IDH2 . Sci Rep 10:8677.
Kahancova 2020 Sci Rep2020Kahancová A, Sklenář F, Ježek P, Dlasková A (2020) Overexpression of native IF1 downregulates glucose-stimulated insulin secretion by pancreatic INS-1E cells. Sci Rep 10:1551.
Jezek 2020 Antioxid Redox Signal2020Jezek P (2020) 2-Hydroxyglutarate in cancer cells. Antioxid Redox Signal [ahead of print].
Dlaskova 2019 Biochim Biophys Acta Bioenerg2019Dlasková A, Špaček T, Engstová H, Špačková J, Schröfel A, Holendová B, Smolková K, Plecitá-Hlavatá L, Ježek P (2019) Mitochondrial cristae narrowing upon higher 2-oxoglutarate load. Biochim Biophys Acta Bioenerg 1860:659-78.
Jezek 2018 Antioxid Redox Signal2018Jezek P, Holendova B, Garlid KD, Jaburek M (2018) Mitochondrial uncoupling proteins: subtle regulators of cellular redox signaling. Antioxid Redox Signal 29:667-714.
Kahancova 2018 FEBS Lett2018Kahancova A, Sklenar F, Jezek P, Dlaskova A (2018) Regulation of glucose-stimulated insulin secretion by ATPase Inhibitory Factor 1 (IF1). FEBS Lett 592:999-1009.
Jezek 2017 Biochim Biophys Acta2017Ježek J, Engstová H, Ježek P (2017) Antioxidant mechanism of mitochondria-targeted plastoquinone SkQ1 is suppressed in aglycemic HepG2 cells dependent on oxidative phosphorylation. Biochim Biophys Acta 1858:750-62.
Rohlenova 2016 Antioxid Redox Signal2016Rohlenova K, Sachaphibulkij K, Stursa J, Bezawork-Geleta A, Blecha J, Endaya B, Werner L, Cerny J, Zobalova R, Goodwin J, Spacek T, Alisadeh E, Yan B, Nguyen M, Vondrusova M, Sobol M, Jezek P, Hozak P, Truksa J, Rohlena J, Dong L, Neuzil J (2016) Selective disruption of respiratory supercomplexes as a new strategy to suppress Her2high breast cancer. Antioxid Redox Signal 26:84-103.
Plecita-Hlavata 2015 Am J Respir Cell Mol Biol2015Plecitá-Hlavatá L, Tauber J, Li M, Zhang H, Flockton AR, Pullamsetti SS, Chelladurai P, D'alessandro A, El Kasmi KC, Ježek P, Stenmark KR (2015) Constitutive reprogramming of fibroblast mitochondrial metabolism in pulmonary hypertension. Am J Respir Cell Mol Biol 55:47-57.
Plecita-Hlavata 2015 J Bioenerg Biomembr2015Plecitá-Hlavatá L, Ježek J, Ježek P (2015) Aglycemia keeps mitochondrial oxidative phosphorylation under hypoxic conditions in HepG2 cells. J Bioenerg Biomembr 47(6):467-76.
Smolkova 2015 Int J Biochem Cell Biol2015Smolková K, Dvořák A, Zelenka J, Vítek L, Ježek P (2015) Reductive carboxylation and 2-hydroxyglutarate formation by wild-type IDH2 in breast carcinoma cells. Int J Biochem Cell Biol 65:125-33.
Jezek 2015 Antioxid Redox Signal2015Ježek J, Dlasková A, Zelenka J, Jabůrek M, Ježek P (2015) H2O2-activated mitochondrial phospholipase iPLA2γ prevents lipotoxic oxidative stress in synergy with UCP2, amplifies signaling via G-protein-coupled receptor GPR40, and regulates insulin secretion in pancreatic β-cells. Antioxid Redox Signal 23:958-72.
Jaburek 2013 Int J Biochem Cell Biol2013Jabůrek M, Ježek J, Zelenka J, Ježek P (2013) Antioxidant activity by a synergy of redox-sensitive mitochondrial phospholipase A2 and uncoupling protein-2 in lung and spleen. Int J Biochem Cell Biol 45:816-25.
Jezek 2011 AbstractMitoComLectures2011Jezek P, Dlaskova A, Santorova J, Spacek T, Smolkova K, Tauber J, Mlodzianoski MJ, Bewersdorf J (2011) 3D visualization of mitochondrial network and mtDNA nucleoids at 30 nm by biplane FPALM microscopy. Abstract MitoCom Lectures.
Smolkova 2010 J Bioenerg Biomembr2010Smolková K, Bellance N, Scandurra F, Génot E, Gnaiger E, Plecitá-Hlavatá L, Ježek P, Rossignol R (2010) Mitochondrial bioenergetic adaptations of breast cancer cells to aglycemia and hypoxia.
Jezek 2010 Physiol Res2010Ježek J, Jabůrek M, Zelenka J, Ježek P (2010) Mitochondrial phospholipase A2 activated by reactive oxygen species in heart mitochondria induces mild uncoupling. Physiol Res 59:737-47.
Plecita-Hlavata 2009 Int J Biochem Cell Biol2009Plecita-Hlavata L, Jezek J, Jezek P (2009) Pro-oxidant mitochondrial matrix-targeted ubiquinone MitoQ10 acts as anti-oxidant at retarded electron transport or proton pumping within Complex I. Int J Biochem Cell Biol 41:1697-707.
Spacek 2008 Int J Biochem Cell Biol2008Spacek T, Santorová J, Zacharovová K, Berková Z, Hlavatá L, Saudek F, Jezek P (2008) Glucose-stimulated insulin secretion of insulinoma INS-1E cells is associated with elevation of both respiration and mitochondrial membrane potential. Int J Biochem Cell Biol 40:1522-35.
Hlavata 2008 Aging Cell2008Hlavatá L, Nachin L, Jezek P, Nyström T (2008) Elevated Ras/protein kinase A activity in Saccharomyces cerevisiae reduces proliferation rate and lifespan by two different reactive oxygen species-dependent routes. Aging Cell 7:148-57.
Dlaskova 2008 Int J Biochem Cell Biol2008Dlasková A, Hlavatá L, Jezek J, Jezek P (2008) Mitochondrial Complex I superoxide production is attenuated by uncoupling. Int J Biochem Cell Biol 40:2098-109.
Zacharovova 2005 Transplant Proc2005Zacharovova K, Berkova Z, Spacek T, Kriz J, Dovolilova E, Girman P, Koblas T, Jezek P, Saudek F (2005) In vitro assessment of pancreatic islet vitality by oxymetry. Transplant Proc 37:3454-6.


Jaburek 2014 Abstract MiP20142014The role of phospholipase A2γ in the regulation of mitochondrial uncoupling protein 2–dependent antioxidant function.
Jezek 2014 Abstract MiP20142014Mitochondrial cristae morphology changes: from Hackenbrock to hypoxia.
Jezek 2013 Abstract MiP20132013Ježek P, Ježek J, Zelenka J, Dlasková A, Jabůrek M (2013) Antioxidant synergy of mitochondrial uncoupling protein UCP2 and phospholipase iPLA2γ. Mitochondr Physiol Network 18.08.
Dlaskova 2013 Abstract MiP20132013Dlasková A, Tauber J, Špaček T, Alán L, Ježek P (2013) Distribution of nucleoids of mitochondrial DNA. Mitochondr Physiol Network 18.08.
Pajuelo-Reguera 2013 Abstract MiP20132013Pajuelo DR, Ježek P, Olejár T, Alán L (2013) Some mitophagy markers in liver and skeletal muscle in Goto Kakizaki rats. Mitochondr Physiol Network 18.08.
Plecita-Hlavata 2013 Abstract MiP20132013Plecita-Hlavata L, Engstova H, Spacek T, Alan L, Stradalova V, Malinsky J, Jezek P(2013) Mitochondrial network and cristae remodeling upon hypoxia. Mitochondr Physiol Network 18.08.
Zelenka 2013 Abstract MiP20132013Zelenka J, Smolkova K, Dvorak A, Vitek L, Jezek P (2013) Reverse carboxylation glutaminolysis in breast cancer cells. Mitochondr Physiol Network 18.08.
Zelenka J 2013 Abstract MiP20132013Zelenka J, Alan L, Jezek P(2013) Response of cancer cells to mitochondrial DNA damage. Mitochondr Physiol Network 18.08.

Participated at

CV (Nov 2011)

Born: April 22, 1957; Current job position: Head, Dept. Membrane Transport Biophysics, No.75, Inst.Physiology.

Engaged in the European Union project BIOCEV, Vestec by Prague.

Education: 1981: Faculty of Mathematics and Physics, Charles University, Prague, Czech Republic, graduated in biophysics (title "RNDr" in physics); 1981-1986: a postgraduate fellowship at the Department of Bioenergetics, Institute of Physiology, Acad. Sci., under supervision of Josef Houštek, M.D 1986: Ph.D.-degree in Physiology of animals - Ph.D. thesis: "Ion transport and thermogenesis in brown adipose tissue mitochondria". 2001: achieved D.Sc.-degree (doctor scienciarum) in Physiology of animals (Acad.Sciences and Charles University, Prague). DSc. Thesis: „Mitochondrial uncoupling proteins, anion carriers and ion channels.“

Postdoctoral research training: 1987 Germany - lab. of Prof. Klaus Ring, President Univ. Frankfurt/Main, FRG. 1988-1990: U.S.A., Advanced postdoctoral fellowships in the lab. of Prof. K.D. Garlid at the Dept. Pharmacology, Medical College of Ohio, Toledo, Ohio, U.S.A. 1992: CSIC, CIB, Institute of Biology, Madrid - 8 months. Research and academic positions:

Since 1991: Head of the Department, permanent position of researcher in the Institute of Physiology, Prague; February 1991 formed own Department of Membrane transport Biophysics in this Institute, building completely new laboratory for biofluorescence methods and molecular biology up to present. Recently, he is developing the 3D superresolution microscopy, such as 4Pi microscopy and BiplaneFPALM microscopy.

Dr. P.J. is member of four commissions organizing Ph.D. studies in Czech Republic, supervising many Ph.D. students, several have already graduated under his supervision in biochemistry and biophysics, respectively. Memberships in Editorial Boards: member of the editorial board of the Int.J. Biochem.& Cell Biology (London).

Past achievement: Dr. P. J.: h-index 29; till 2010: >3000 citations, published 91 major scientific papers (total impact factor 351.5, i.e. 13.5 per year since 1984, an average IF 3.86 per paper; among which 18 appeared in the Journal of Biological Chemistry (2 in FASEB J., 1 in Aging Cell, 15 in IJBCB, 6 in BBA, 16 in FEBS Lett., 6 in J. Bioenerg. Biomembr., 3 in Eur. J. Bioch., 2 in Am. J. Physiol., etc.). One Czech and PCT patent (EU applied) in photodynamic therapy of tumors.

Dr. PJ has received numerous foreign grants such as Fogarty Int. grant 1992-1995, & Prof.Garlid, OGI. Portland OR, USA; grant of Czech&US Science & Technology Program 1996-1998, Prof.Garlid, OGI Portland OR, USA; DFG grant 1996-1997, (& Dr. D. Siemen, Univ. Kiel), PRONEX - Brazilian Federal Grant for the Excellence centers – 1998-2002 PIs: Prof. Vercesi, Arruda UNICAMP; DFG grant 2000-2001, (& Dr. P. Pohl, Univ. Halle, SRN); Fogarty International grant 2002-2005,& Garlid, Portland State University, OR, USA. Dr. P.J. has been developing (&colleagues) for past 10 years numerous techniques of modern mol. biology and recently superresolution 3Dimensional fluorescence microscopy.

List of milestone papers (antichronologically): (C = citations without autocitations)

  • Mlodzianoski MJ, Schreiner JM, Callahan SP, Smolková K, Dlasková A, Šantorová J, Ježek P, Bewersdorf J (2011) Sample drift correction in 3D fluorescence photoactivation localization microscopy. Opt. Express. 19: 15009-15019. IF 3.749
  • Dlasková A, Špaček T, Šantorová J, Plecitá-Hlavatá L, Berková Z, Saudek F, Lessard M, Bewersdorf J, Ježek P (2010) 4Pi microscopy reveals an impaired three-dimensional mitochondrial network of pancreatic islet -cells in an experimental model of type-2 diabetes. Biochim. Biophys Acta, Bioenergetics 1797: 1327-1341. IF 3.688
  • Plecitá-Hlavatá L, Lessard M, Šantorová J, Bewersdorf J, Ježek P (2008) Mitochondrial oxidative phosphorylation and energetic status are reflected by morphology of mitochondrial network in INS-1E and HEP-G2 cells viewed by 4Pi microscopy. Biochim. Biophys. Acta 1777: 834–846. IF 4.678 C=17
  • Dlasková A, Hlavatá L, Ježek P (2008) Oxidative stress caused by blocking of mitochondrial Complex I H+ pumping as a link in aging/disease vicious cycle. Int. J. Biochem. Cell Biol. 40: 1792–1805. IF 4.804 C=10 Indication of excessive superoxide production in living cells
  • Garlid KD et al (1996) On the mechanism of fatty acid-induced proton transport by mitochondrial uncoupling protein J. Biol. Chem. 271: 2615-2620. IF 7.452 C=180
  • Jabůrek M, Vařecha M, Gimeno RE, Dembski M, Ježek P, Zhang M, Burn P, Tartaglia LA, Garlid KD (1999) Transport function and regulation of mitochondrial uncoupling proteins 2 and 3. J. Biol. Chem. 274: 26003-26007. IF 7.666 C=189 Proof of fatty acid cycling mechanism for UCP2
  • Ježek P et al (1994) Transport of anions and protons by the mitochondrial uncoupling protein and its regulation by nucleotides and fatty acids: A new look at old hypotheses. J. Biol. Chem. 269: 26184-26190. IF 7.666 C=73 Proof of fatty acid cycling mechanism
  • Ježek P et al (1990) Reconstitution of the beef heart and rat liver mitochondrial K+/H+ (Na+/H+) antiporter: Quantification of K+ transport with the novel fluorescent probe PBFI. J. Biol. Chem. 265: 10522-10526. IF 7.666 C=47 Intro of fluorescent cation indicator method for reconstitution assays
  • Ježek P, Garlid KD (1990) New substrates and competitive inhibitors of the Cl- translocating pathway of the uncoupling protein of brown adipose tissue mitochondria. J. Biol. Chem. 265: 19303-19311. IF 7.666 C=64 Novel transport substrates of uncoupling protein revealed
  • Ježek P et al (1990) Reconstitution of the uncoupling protein of brown adipose tissue mitochodria: Demonstration of GDP-sensitive halide anion uniport. J. Biol. Chem. 265: 19296-19302. IF 7.666 C=32 Introduction of fluorescent anion indicator method for reconstitution assays
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