MiPNet22.04 IOC120 Barcelona ES
 Barcelona ES, 2017 Mar 20. O2k-Workshop on high-resolution respirometry (HRR) IOC120. »ES Barcelona Zorzano A |
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Oroboros (2017-03-20) Mitochondr Physiol Network
Abstract:
O2k-Workshop on high-resolution respirometry - satellite to MitoEAGLE Barcelona 2017. Barcelona, Spain; 2017 Mar 20.
• O2k-Network Lab: AT_Innsbruck_Oroboros, ES Barcelona Zorzano A
General information
- O2k-Fluorometer demo experiment: high-resolution respirometry and H2O2 production (Amplex red)
- Followed by the Barcelona MitoEAGLE Conference, MG and WC Meeting.
Programme
| Monday 20th | Topic | Weblink |
| 08:45 | Registration | |
| 09:00-09:15 | Welcome by Prof. Antonio Zorzano. | |
| 09:15-09:30 | Introduction of participants and their research interests. | IOC120 |
| 09:30-10:00 | Get started with the O2k: Overview with video clips. | O2k-Manual |
| 10:00-11:30 | Comprehensive OXPHOS analysis: substrate-uncoupler-inhibitor titration (SUIT) protocols for respiratory control by coupling and mitochondrial pathways, SUIT reference assay. | The Blue Book - SUIT reference protocol |
| 11:30-12:00 | Coffee/tea break | |
| 12:00-13:00 | Principles of high-resolution respirometry: From switching
on the O2k to the experimental result – oxygen sensor calibration and quality control. |
POS-calibration-SOP |
| 13:00-14:00 | O2k-Fluorometry and OXPHOS analysis: Amplex red assay of H2O2 production and design of experimental protocol. | O2k-Fluorometry |
| 14:00-15:00 | Lunch | |
| 15:00-17:30 | Demo-Experiment: HRR and O2k-Fluorometry with liver mitochondria – respiration and H2O2 production applying the SUIT reference assay: Protocol driver for RP1&RP2. | RP1 - RP2 |
| 17:30-18:30 | Protocol driver for Marks and data analysis. | |
| 18:30-20:00 | The Bioblast wiki, O2k-Network and feedback discussion. |
Venue
- Barcelona Science Park
- Room 1 Tower D
- R+D+I Towers building
- C/ Baldiri Reixac 4-8
- 08028 Barcelona
- To get to the workhsop location following routes are possible:
- Start in Barcelona centrum: easiest way is to take the Metro Line 3 (L3) to Palau Reial and walk approximately 5 min to the final destination
- Another option is to take a bus(see picture below with bus plan)
- To get to the workhsop location following routes are possible:
- Start airport: take the Metro Line 9 (L9) to Zona Universitaria, which will take around 30 min
- Taxi will take about 20 min and the costs are approximately 20-25 €
- Another option is the train (Renfe to Sants Station) and then there the Metro L3 to Palau Reial
Registration
- Please register here for the O2k-Workshop.
- Registration deadline: March 13
Lecturers and tutors
List of participants
Participant Institution 
Barros Susana ES_Barcelona_Zorzano A: IRB Barcelona (ES) Martín Cabezuelo Rubén Universitat Politècnica de València (UPV) Gama Perez Pau ES Barcelona Garcia-Roves PM : Physiological Science Department, University of Barcelona (ES) Garten Antje UK_Birmingham_Lavery GG: Institute of Metabolism and Systems Research, University of Birmingham (UK) Giovarelli Matteo IT_Milan_Clementi_E: Department of Biomedical and Clinical Sciences University of Milan, University of Milan (IT) Gonzalo Hugo ES Lleida Boada J: IRB Lleida (ES) Granado Serrano Ana Belén ES Lleida Boada J: IRB Barcelona (ES) 
Herrero Laura Dpt. Biochemistry. School of Pharmacy. Univ. Barcelona, University of Barcelona (ES) Iglesias-Gonzalez Javier UK_Manchester_Galli GL: Division of Cell Matrix Biology and Regenerative Medicine, University of Manchester (UK) 
Ingram Thomas University of Nottingham (UK) 
Koenig Magdalena Goethe University, Frankfurt (DE) Lyakhovich Alexander Research Institute VHIR, Barcelona (ES) 
Nemec Michal Institute of Experimental Endocrinology, Bratislava (SK) Nogueras Lara ES Lleida Boada J: IRB Lleida (ES) 
Ost Mario DE Nuthetal Klaus S: Department of Pharmacology, German Institute of Human Nutrition (DE) Rivera-Ingraham Georgina Université de Montpellier (FR) Sabate Alba ES Barcelona Zorzano A: IRB Barcelona (ES) Sánchez Feutrie Manuela ES Barcelona Zorzano A: Department of Biochemistry and Molecular Biology, University of Barceona (ES) 
Sebastián David ES_Barcelona_Zorzano A: IRB Barcelona (ES) Soler Vázquez Maria Del Carmen Department of Biochemistry and Physiology, University of Barcelona (ES) 
Thinnes Anna Goethe University, Frankfurt (DE) Zagmutt Caroca Sebastián University of Barcelona (ES)
Contact
- Oroboros Instruments
- high-resolution respirometry
- Schöpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
Project support
- Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
IOC recommended reading
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.
Labels:
HRR: Oxygraph-2k
ORO, 2017, IOC, O2k-Network
