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Giakoumaki 2019 MiPschool Coimbra

From Bioblast
MiPsociety
Mitochondrial respiration in human skeletal muscle after 60 days of best rest.

Link: MitoEAGLE

Giakoumaki I, Degens H, Wuest R (2019)

Event: MiPschool Coimbra 2019

COST Action MitoEAGLE

Long-term inactivity has been associated with skeletal muscle atrophy, exercise tolerance and glucose intolerance [1,2]. Previous studies have shown a positive effect of short-term immobility on mitochondrial respiration of young individuals [3,4], but it remains to be established to what extent long-term bed rest induces qualitative and quantitative alterations in mitochondrial function in skeletal muscle.

This study aims to investigate the impact of long-term inactivity on mitochondrial respiration capacity in skeletal muscle of young men and women. Healthy volunteers (8 men and 4 women, between 22-55 years old) underwent 60 days of bed rest. Skeletal muscle biopsies were obtained from the vastus lateralis muscle at day -1, 5 and 55. Approximately 12 mg of each muscle biopsy was mechanically separated and permeabilised, followed by assessment of mitochondrial respiration using high-resolution respirometry (Oroboros Oxygraph O2k, Innsbruck, Austria). Currently, the data is under analysis and the first results are expected during summer 2019.

Frozen parts from the same biopsies will be used to assess mitochondrial morphology (by electron microscopy and immunohistochemistry) and protein concentration of key metabolic proteins to better understand the underlying cause of the inactivity-induced exercise tolerance and insulin resistance.


Bioblast editor: Plangger M


Labels: MiParea: Respiration, Exercise physiology;nutrition;life style 


Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 



HRR: Oxygraph-2k 


Affiliations

Giakoumaki I(1), Degens H(1), Wüst R(2)
  1. Dep Musculoskeletal Science Sports Medicine, Univ Manchester Metropolitan, Manchester, UK
  2. Dep Human Movement Sciences, Univ Manchester Metropolitan, Amsterdam, Nederlands. - [email protected]

References

  1. Rudwill F, O’gorman D, Lefai E, Chery I, Zahariev A, Normand S, Pagano AF, Chopard A, Damiot A, Laurens C, Hodson L (2018) Metabolic inflexibility is an early marker of bed-rest–induced glucose intolerance even when fat mass is stable. J Clin Endocrinol Metab 103:1910-20.
  2. Parry SM, Puthucheary ZA (2015) The impact of extended bed rest on the musculoskeletal system in the critical care environment. Extrem Physiol Med 4:16.
  3. Kenny HC, Rudwill F, Breen L, Salanova M, Blottner D, Heise T, Heer M, Blanc S, O’gorman DJ (2017) Bed rest and resistive vibration exercise unveil novel links between skeletal muscle mitochondrial function and insulin resistance. Diabetologia 60:1491-501.
  4. Larsen S, Lundby AK, Dandanell S, Oberholzer L, Keiser S, Andersen AB, Haider T, Lundby C (2018) Four days of bed rest increases intrinsic mitochondrial respiratory capacity in young healthy males. Physiol Rep 6:e13793.