Fecker 2020 Biomolecules
Fecker R, Buda V, Alexa E, Avram S, Pavel IZ, Muntean D, Cocan I, Watz C, Minda D, Dehelean CA, Soica C, Danciu C (2020) Phytochemical and biological screening of Oenothera biennis L. hydroalcoholic extract. Biomolecules 10:E818. |
Fecker R, Buda V, Alexa E, Avram S, Pavel IZ, Muntean D, Cocan I, Watz C, Minda D, Dehelean CA, Soica C, Danciu C (2020) Biomolecules
Abstract: Oenothera biennis L. (OB), also commonly known as evening primrose, belongs to the Onagraceae family and has the best studied biological activity of all the members in the family. In therapy, the most frequently used type of extracts are from the aerial part, which are the fatty oils obtained from the seeds and have a wide range of medicinal properties. The aim of this study was to evaluate the phytochemical composition and biological activity of OB hydroalcoholic extract and to provide directions for the antimicrobial effect, antiproliferative and pro-apoptotic potential against A375 melanoma cell line, and anti-angiogenic and anti-inflammatory capacity. The main polyphenols and flavonoids identified were gallic acid, caffeic acid, epicatechin, coumaric acid, ferulic acid, rutin and rosmarinic acid. The total phenolic content was 631.496 Β΅gGAE/mL of extract and the antioxidant activity was 7258.67 ΞΌmolTrolox/g of extract. The tested extract had a mild bacteriostatic effect on the tested bacterial strains. It was bactericidal only against Candida spp. and S. aureus. In the set of experimental conditions, the OB extract only manifested significant antiproliferative and pro-apoptotic activity against the A375 human melanoma cell line at the highest tested concentration, namely 60 ΞΌg/mL. The migration potential of A375 cells was hampered by the OB extract in a concentration-dependent manner. Furthermore, at the highest tested concentration, the OB extract altered the mitochondrial function in vitro, while reducing the angiogenic reaction, hindering compact tumor formation in the chorioallantoic membrane assay. Moreover, the OB extract elicited an anti-inflammatory effect on the experimental animal model of ear inflammation. β’ Keywords: Oenothera biennis L. hydroalcoholic extract, Angiogenesis, Antibacterial, Antiproliferative, Chorioallantoic membrane, Inflammation, Mitochondrial function, Pro-apoptotic β’ Bioblast editor: Plangger M β’ O2k-Network Lab: RO Timisoara Muntean DM
Labels: MiParea: Respiration, Pharmacology;toxicology
Stress:Cell death Organism: Human Tissue;cell: Endothelial;epithelial;mesothelial cell Preparation: Permeabilized cells, Intact cells
Coupling state: LEAK, ROUTINE, OXPHOS, ET
Pathway: N, S, NS, ROX
HRR: Oxygraph-2k
2020-06