Dos Santos Escaliante 2021 Int J Biol Macromol
| Dos Santos Escaliante LA, Busato B, de Oliveira Petkowicz CL, Suter Correia Cadena SM, Rodrigues Noleto G (2021) Cytotoxic effect of xyloglucan and oxovanadium (IV/V) xyloglucan complex in HepG2 cells. Int J Biol Macromol 185:40-48. |
Dos Santos Escaliante Leonardo Augusto, Busato Bianca, de Oliveira Petkowicz Carmen Lucia, Suter Correia Cadena Silvia Maria, Rodrigues Noleto Guilhermina (2021) Int J Biol Macromol
Abstract: It is well known that the chemical structure of polysaccharides is important to their final biological effect. In this study we investigated the cytotoxic effect of xyloglucan from Copaifera langsdorffii seeds (XGC) and its complex with oxovanadium (XGC:VO) on hepatocellular carcinoma cells (HepG2). After 72 h of incubation, XGC and XGC:VO (200 ΞΌg/mL) reduced cell viability in ~20% and ~40%, respectively. At same conditions, only XGC:VO increased in ~20% the LDH enzyme release. In permeabilized cells, incubated with XGC and XGC:VO (200 ΞΌg/mL) for 72 h, NADH oxidase activity was reduced by ~45% with XGC and XGC:VO. The succinate oxidase activity was reduced by ~35% with XGC and ~65% with XGC:VO, evidencing that polysaccharide complexation with vanadium could intensify its effects on the respiratory chain. According to this result, the mitochondrial membrane potential was also reduced by ~9% for XGC and ~30% for XGC:VO, when compared to the control group. Interestingly, ATP levels were more elevated for XGC:VO in respect to XGC, probably due the enhance in glycolytic flux evidenced by increased levels of lactate. These results show that the xyloglucan complexation with oxovanadium (IV/V) potentiates the cytotoxic effect of the native polysaccharide, possibly by impairment of oxidative phosphorylation. β’ Keywords: HepG2 cells, Vanadium, Xyloglucan β’ Bioblast editor: Plangger M
Labels: MiParea: Respiration, Pharmacology;toxicology
Organism: Human
Tissue;cell: Liver
Preparation: Permeabilized cells
Coupling state: LEAK, OXPHOS
Pathway: N, S, CIV, ROX
HRR: Oxygraph-2k
2021-07
