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De Paula Nascimento-Castro 2022 Biomedicines

From Bioblast
Publications in the MiPMap
de Paula Nascimento-Castro C, Winkelmann-Duarte EC, Mancini G, Welter PG, Plácido E, Farina M, Gil-Mohapel J, Rodrigues ALS, de Bem AF, Brocardo PS (2022) Temporal characterization of behavioral and hippocampal dysfunction in the YAC128 mouse model of Huntington's disease.

» Biomedicines 10:1433. PMID: 35740454 Open Access

de Paula Nascimento-Castro Cristine,  Winkelmann-Duarte Elisa C,  Mancini Gianni,  Gomes Welter Priscilla,  Placido Evelini, Farina Marcelo,  Gil-Mohapel Joana,  Rodrigues Ana Lucia S,  De Bem Andreza Fabro,  Brocardo Patricia S (2022) Biomedicines

Abstract: Huntington's disease (HD) is a genetic neurodegenerative disease characterized by motor, psychiatric, and cognitive symptoms. Emerging evidence suggests that emotional and cognitive deficits seen in HD may be related to hippocampal dysfunction. We used the YAC128 HD mouse model to perform a temporal characterization of the behavioral and hippocampal dysfunctions. Early and late symptomatic YAC128 mice exhibited depressive-like behavior, as demonstrated by increased immobility times in the Tail Suspension Test. In addition, YAC128 mice exhibited cognitive deficits in the Swimming T-maze Test during the late symptomatic stage. Except for a reduction in basal mitochondrial respiration, no significant deficits in the mitochondrial respiratory rates were observed in the hippocampus of late symptomatic YAC128 mice. In agreement, YAC128 animals did not present robust alterations in mitochondrial ultrastructural morphology. However, light and electron microscopy analysis revealed the presence of dark neurons characterized by the intense staining of granule cell bodies and shrunken nuclei and cytoplasm in the hippocampal dentate gyrus (DG) of late symptomatic YAC128 mice. Furthermore, structural alterations in the rough endoplasmic reticulum and Golgi apparatus were detected in the hippocampal DG of YAC128 mice by electron microscopy. These results clearly show a degenerative process in the hippocampal DG in late symptomatic YAC128 animals. Keywords: Huntington’s disease, YAC128 mice, Electron microscopy, Hippocampus, Neurodegeneration Bioblast editor: Plangger M O2k-Network Lab: BR Brasilia De Bem AF

Labels: MiParea: Respiration  Pathology: Neurodegenerative 

Organism: Mouse  Tissue;cell: Nervous system  Preparation: Homogenate 

Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, NS, ROX  HRR: Oxygraph-2k