Damgaard 2022 iScience

Damgaard MV, Nielsen TS, Basse AL, Chubanava S, Trost K, Moritz T, Dellinger RW, Larsen S, Treebak JT (2022) Intravenous nicotinamide riboside elevates mouse skeletal muscle NAD+ without impacting respiratory capacity or insulin sensitivity. iScience 25:103863. https://doi.org/10.1016/j.isci.2022.103863

» PMID: 35198907 Open Access

Damgaard Mads V, Nielsen Thomas S,  Basse Astrid L,  Chubanava Sabina,  Trost Kajetan, Moritz Thomas,  Dellinger Ryan W, Larsen Steen,  Treebak Jonas T (2022) iScience

Abstract: In clinical trials, oral supplementation with nicotinamide riboside (NR) fails to increase muscle mitochondrial respiratory capacity and insulin sensitivity but also does not increase muscle NAD⁺ levels. This study tests the feasibility of chronically elevating skeletal muscle NAD⁺ in mice and investigates the putative effects on mitochondrial respiratory capacity, insulin sensitivity, and gene expression. Accordingly, to improve bioavailability to skeletal muscle, we developed an experimental model for administering NR repeatedly through a jugular vein catheter. Mice on a Western diet were treated with various combinations of NR, pterostilbene (PT), and voluntary wheel running, but the metabolic effects of NR and PT treatment were modest. We conclude that the chronic elevation of skeletal muscle NAD⁺ by the intravenous injection of NR is possible but does not affect muscle respiratory capacity or insulin sensitivity in either sedentary or physically active mice. Our data have implications for NAD⁺ precursor supplementation regimens. • Keywords: Drugs, Molecular physiology, Transcriptomics • Bioblast editor: Plangger M • O2k-Network Lab: DK Copenhagen Larsen S

Correction: FADH₂ and Complex II

FADH₂ is shown as the substrate feeding electrons into Complex II (CII). This is wrong and requires correction - for details see Gnaiger (2024).

Gnaiger E (2024) Complex II ambiguities ― FADH₂ in the electron transfer system. J Biol Chem 300:105470. https://doi.org/10.1016/j.jbc.2023.105470 - »Bioblast link«

Labels: MiParea: Respiration, Exercise physiology;nutrition;life style 

Organism: Mouse  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 

Coupling state: LEAK, OXPHOS  Pathway: N, NS, ROX  HRR: Oxygraph-2k 

2022-12