Cormier 2021 Insect Biochem Mol Biol

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Cormier RJ, Strang R, Menail H, Touaibia M, Pichaud N (2021) Systemic and mitochondrial effects of metabolic inflexibility induced by high fat diet in Drosophila melanogaster. Insect Biochem Mol Biol [Epub ahead of print].

» PMID: 33626368

Cormier Robert J, Strang Rebekah, Menail Hichem, Touaibia Mohamed, Pichaud Nicolas (2021) Insect Biochem Mol Biol

Abstract: Metabolic inflexibility is a condition that occurs following a nutritional stress which causes blunted fuel switching at the mitochondrial level in response to hormonal and cellular signalling. Linked to obesity and obesity related disorders, chronic exposure to a high fat diet (HFD) in animal models has been extensively used to induce metabolic inflexibility and investigate the development of various metabolic diseases. However, many questions concerning the systemic and mitochondrial responses to metabolic inflexibility remain. In this study, we investigated the global and mitochondrial variations following a 10-day exposure to a HFD in adult Drosophila melanogaster. Our results show that following 10-day exposure to the HFD, mitochondrial respiration rates measured in isolated mitochondria at the level of complex I were decreased. This was associated with increased contributions of non-classical providers of electrons to the electron transport system (ETS) such as the proline dehydrogenase (ProDH) and the mitochondrial glycerol-3-phosphate dehydrogenase (mtG3PDH) alleviating complex I dysfunctions, as well as with increased ROS production per molecule of oxygen consumed. Our results also show an accumulation of metabolites from multiple different metabolic pathways in whole adult Drosophila and a drastic shift in the lipid profile which translated into decreased proportion of saturated and monounsaturated fatty acids combined with an increased proportion of polyunsaturated fatty acids. Thus, our results demonstrate the various responses to the HFD treatment in adult Drosophila melanogaster that are hallmarks of the development of metabolic inflexibility and reinforce this organism as a suitable model for the study of metabolic disorders.

Keywords: Drosophila, Lipid profile, Metabolism, Metabolomics, Mitochondrial glycerol-3-phosphate dehydrogenase, Mitochondrial respiration, Reactive oxygen species Bioblast editor: Plangger M O2k-Network Lab: CA Moncton Hebert-Chatelain E


Labels: MiParea: Respiration, Exercise physiology;nutrition;life style  Pathology: Obesity 

Organism: Drosophila 




HRR: Oxygraph-2k 

2021-03