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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
Cizmarova Beata
MitoPedia topics: EAGLE
COST WG4: WG4
COST ECI: ECI
| Name | Čižmárová (Velika) Beata, PhD, RNDr. |
|---|---|
| Institution |
Pavol Jozef Šafárik University in Kosice
Visiting scientist
Beata Cizmarova joined Oroboros in January 2017 within the framework
|
| Address | Trieda SNP 1, 040 11 |
| City | Košice |
| State/Province | |
| Country | Slovakia |
| [email protected] | |
| Weblink | |
| O2k-Network Lab |
Labels:
Publications
| Published | Reference | |
|---|---|---|
| Evinova 2020 J Membr Biol | 2020 | Evinova A, Cizmarova B, Hatokova Z, Racay P (2020) High‑resolution respirometry in assessment of mitochondrial function in neuroblastoma SH‑SY5Y intact cells. J Membr Biol 253:129-36. |
| MitoEAGLE blood cells 1 | 2020 | Åsander Frostner Eleonor*, Aburel Oana M*, Avram Vlad F*, Calabria Elisa, Castelo Rueda Maria Paulina*, Chamkha Imen*, Čižmárová Beata, Danila Maria-Daniela*, Doerrier Carolina*, Eckert Gunter P*, Ehinger Johannes K, Elmer Eskil*, Garcia-Souza Luiz F*, Gnaiger Erich*, Hoppel Florian*, Karabatsiakis Alexander*, Keppner Gloria, Kidere Dita*, Krako Jakovljević Nina, Labieniec-Watala Magdalena*, Lelcu Theia*, Micankova Petra, Michalak Slawomir*, Molina Anthony JA*, Pavlovic Kasja, Pichler Irene*, Piel Sarah, Rousar Tomas, Rybacka-Mossakowska Joanna, Schartner Melanie, Siewiera Karolina*, Silaidos Carmina*, Sjövall Fredrik*, Sobotka Ondrej*, Sumbalova Zuzana*, Swiniuch Daria, Vernerova Andrea*, Volani Chiara*, Vujacic-Mirski Ksenija*, Watala Cezary* (2020) Interlaboratory guide to mitochondrial respiratory studies with peripheral blood mononuclear cells and platelets. - Updated: 2020-03-06 - *Confirmed |
| BEC 2020.1 doi10.26124bec2020-0001.v1 | 2020 | Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1 |
| Gatterer 2018 J Sports Sci Med | 2018 | Gatterer H, Menz V, Salazar-Martinez E, Sumbalova Z, Garcia-Souza LF, Cizmarova B, Gnaiger E, Burtscher M (2018) Exercise performance, muscle oxygen extraction and blood cell mitochondrial respiration after repeated-sprint and sprint interval training in hypoxia: a pilot study. J Sports Sci Med 17:339-347. |
| Sumbalova 2018 Nova Sciences | 2018 | Sumbalová Z, Garcia-Souza LF, Cizmarova B, Volani C, Gnaiger E (2018) Analysis of mitochondrial function in human blood cells. In: Recent advances in mitochondrial medicine and coenzyme Q10. Gvozdjáková A, Cornelissen G, Singh RB eds, Nova Sciences:255-68. |
Abstracts
| Published | Reference | |
|---|---|---|
| Evinova 2019 MiPschool Coimbra | 2019 |  |
| Hatokova 2019 MiPschool Coimbra | 2019 | |
| Garcia-Souza 2018 Life Sciences Meeting 2018 Innsbruck AT | 2018 | A respirometric cell viability test for peripheral-blood mononuclear cells and platelets. |
| Evinova 2018 MiP2018 | 2018 | |
| Cizmarova 2018 MiP2018 | 2018 |  |
| Cizmarova 2017 Abstract MITOEAGLE Barcelona | 2017 |  |
| Laner 2017 Abstract EUROMIT2017 Cologne | 2017 |  |
| Garcia-Souza 2017 MiPschool Obergurgl | 2017 |  |
| Cizmarova 2017 MiPschool Obergurgl | 2017 | |
| Garcia-Souza 2017 MITOEAGLE Obergurgl | 2017 | |
| Cizmarova 2017 MITOEAGLE Obergurgl | 2017 | |
| Cizmarova 2017 MiP2017 | 2017 | |
| Garcia-Souza 2017 MiP2017 | 2017 | |
| Sumbalova 2017 MiP2017 WG4 | 2017 |  |
MitoEAGLE Short-Term Scientific Mission
- Work Plan summary
- My name is Beata Velika and I work as Research specialist at the Pavol Jozef Safarik University in Kosice, Department of Medical and Clinical Biochemistry, Faculty of Medicine, Slovakia. During my study and work I specialized in fluorescence spectroscopy, and I have experience with different isolation methods, including mitochondria isolation, and measuring of their native fluorescence and respiration.
- I would like to carry out the project in cooperation with the research group at the Mitochondrial Research Laboratory of Prof. Dr. Erich Gnaiger in Innsbruck, Austria (Medical University of Innsbruck; K-Regio project MitoFit) in collaboration with the scientific company OROBOROS INSTRUMENTS Corp. The laboratory of Prof. Dr. Erich Gnaiger contains very high standard laboratory equipment, with several specialists in mitochondrial physiology field. The short-term project is focused on the optimization of SUIT (substrate-uncoupler-inhibitor titration) protocols for high-resolution respirometry of intact cryopreserved human peripheral blood mononuclear cells (PBMC). The usage of human PBMC will provide a significant stimulus for the entire field of mitochondrial medicine, because the mitochondrial function of PBMC is linked with numerous diseases and metabolic conditions in the human body, and the protocols developed during the project will lead to the standardization of the procedures, and should be used for studying mitochondria physiological and pathological mechanisms. Cryopreserved cells (stored at -80 °C), will be thawed in 37 °C water bath, dilluted in 10 ml pre-warmed 37 °C medium composed of DPBS + 2%FBS and centrifuged (300 g, 6 minutes). The sediment will be resuspended and counted. The suspension of cryopreserved cells will be titrated into the 2-ml chambers of the Oxygraph-2k (Oroboros Instruments, Innsbruck, Austria). We will optimize the SUIT protocols with defined substrate combinations, uncoupler and inhibitor titrations, which will be applied for the measurement of respiration of the cryopreserved cells. The results will be compared with the respiration of the fresh cells (reference). Obtained results will be presented in 10th MiPschool 2017 MitoEAGLE and MitoEAGLE Workshop WG1-4, Obergurgl AT (2017 Jul 23-30).
- Work Plan summary
MitoEAGLE Inclusiveness Target Countries - Conference Grant
Participated at
Visiting scientist in the Oroboros O2k-Laboratory
Beata Cizmarova : Visiting scientist at the Oroboros O2k-Laboratory
- 2018, January 08 to 19
- 2017, January 15 to July 31
