Chorner 2016 Am J Physiol Regul Integr Comp Physiol

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Chorner Z, Barbeau PA, Castellani L, Wright DC, Chabowski A, Holloway GP (2016) Dietary α-linolenic acid supplementation alters skeletal muscle plasma membrane lipid composition, sarcolemmal FAT/CD36 abundance, and palmitate transport rates. Am J Physiol Regul Integr Comp Physiol 311:R1234-42.

» PMID: 27806984

Chorner Z, Barbeau PA, Castellani L, Wright DC, Chabowski A, Holloway GP (2016) Am J Physiol Regul Integr Comp Physiol

Abstract: The cellular processes influenced by consuming polyunsaturated fatty acids remains poorly defined. Within skeletal muscle, a rate-limiting step in fatty acid oxidation is the movement of lipids across the sarcolemmal membrane, and therefore, we aimed to determine the effects of consuming flaxseed oil high in α-linolenic acid (ALA), on plasma membrane lipid composition and the capacity to transport palmitate. Rats fed a diet supplemented with ALA (10%) displayed marked increases in omega-3 polyunsaturated fatty acids (PUFAs) within whole muscle and sarcolemmal membranes (approximately five-fold), at the apparent expense of arachidonic acid (-50%). These changes coincided with increased sarcolemmal palmitate transport rates (+20%), plasma membrane fatty acid translocase (FAT/CD36; +20%) abundance, skeletal muscle triacylglycerol content (approximately twofold), and rates of whole body fat oxidation (~50%). The redistribution of FAT/CD36 to the plasma membrane could not be explained by increased phosphorylation of signaling pathways implicated in regulating FAT/CD36 trafficking events (i.e., phosphorylation of ERK1/2, CaMKII, AMPK, and Akt), suggesting the increased n-3 PUFA composition of the plasma membrane influenced FAT/CD36 accumulation. Altogether, the present data provide evidence that a diet supplemented with ALA increases the transport of lipids into resting skeletal muscle in conjunction with increased sarcolemmal n-3 PUFA and FAT/CD36 contents.

Copyright © 2016 the American Physiological Society. Keywords: Fatty acid translocase, Lipid transport, Lipids/oxidation, Membranes, Omega-3 fatty acids

O2k-Network Lab: CA Guelph Holloway GP

Labels: MiParea: Respiration, Exercise physiology;nutrition;life style 

Organism: Rat  Tissue;cell: Skeletal muscle  Preparation: Isolated mitochondria 

Regulation: ADP  Coupling state: LEAK, OXPHOS  Pathway: F, N, NS  HRR: Oxygraph-2k