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Chojnacka 2022 Mol Biol Cell

From Bioblast
Publications in the MiPMap
Chojnacka KJ, Elancheliyan P, Mussulini BHM, Mohanraj K, Callegari S, Gosk A, Banach T, GΓ³ral T, Szczepanowska K, Rehling P, Serwa RA, Chacinska A (2022) Ovarian carcinoma immunoreactive antigen-like protein 2 (OCIAD2) is a novel complex III-specific assembly factor in mitochondria. https://doi.org/10.1091/mbc.e21-03-0143

Β» Mol Biol Cell 33:ar29. PMID: 35080992 Open Access

Chojnacka Katarzyna Justyna,  Elancheliyan Praveenraj,  Mussulini Ben Hur Marins,  Mohanraj Karthik,  Callegari Sylvie,  Gosk Aleksandra,  Banach Tomasz,  Goral Tomasz,  Szczepanowska Karolina,  Rehling Peter,  Serwa Remigiusz Adam,  Chacinska Agnieszka (2022) Mol Biol Cell

Abstract: Assembly of the dimeric complex III (CIII2) in the mitochondrial inner membrane is an intricate process in which several accessory proteins are involved as assembly factors. Despite numerous studies, this process has yet to be fully understood. Here we report the identification of human OCIAD2 (ovarian carcinoma immunoreactive antigen-like protein 2) as an assembly factor for CIII2. OCIAD2 was found to be deregulated in several carcinomas and also in some neurogenerative disorders; however, its nonpathological role had not been elucidated. We have shown that OCIAD2 localizes to mitochondria and interacts with electron transport chain (ETC) proteins. Complete loss of OCIAD2 using gene editing in HEK293 cells resulted in abnormal mitochondrial morphology, a substantial decrease of both CIII2 and supercomplex III2+IV, and a reduction in CIII enzymatic activity. Identification of OCIAD2 as a protein required for assembly of functional CIII2 provides a new insight into the biogenesis and architecture of the ETC. Elucidating the mechanism of OCIAD2 action is important both for the understanding of cellular metabolism and for an understanding of its role in malignant transformation.

β€’ Bioblast editor: Plangger M


Labels: MiParea: Respiration, Genetic knockout;overexpression 


Organism: Human  Tissue;cell: HEK  Preparation: Permeabilized cells, Intact cells  Enzyme: Complex III 

Coupling state: LEAK, ROUTINE, OXPHOS, ET  Pathway: F, N, S, Gp, CIV, NS, ROX  HRR: Oxygraph-2k 

2022-12