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Cano-Sanchez 2022 PLoS Negl Trop Dis

From Bioblast
Publications in the MiPMap
Cano-Sanchez M, Ben-Hassen K, Louis OP, Dantin F, Gueye P, Roques F, Mehdaoui H, Resiere D, Neviere R (2022) Bothrops lanceolatus snake venom impairs mitochondrial respiration and induces DNA release in human heart preparation. https://doi.org/10.1371/journal.pntd.0010523

ยป PLoS Negl Trop Dis 16:e0010523. PMID: 35727836 Open Access

Cano-Sanchez Mariola, Ben-Hassen Kais, Louis Olivier Pierre, Dantin Fabienne, Gueye Papa, Roques Francois, Mehdaoui Hossein, Resiere Dabor, Neviere Remi (2022) PLoS Negl Trop Dis

Abstract: Envenomations by Bothrops snakebites can induce overwhelming systemic inflammation ultimately leading to multiple organ system failure and death. Release of damage-associated molecular pattern molecules (DAMPs), in particular of mitochondrial origin, has been implicated in the pathophysiology of the deregulated innate immune response.

The objective was to test whether whole Bothrops lanceolatus venom would induce mitochondrial dysfunction and DAMPs release in human heart preparations.

Human atrial trabeculae were obtained during cannulation for cardiopulmonary bypass from patients who were undergoing routine coronary artery bypass surgery. Cardiac fibers were incubated with vehicle and whole Bothrops lanceolatus venom for 24hr before high-resolution respirometry, mitochondrial membrane permeability evaluation and quantification of mitochondrial DNA.

Compared with vehicle, incubation of human cardiac muscle with whole Bothrops lanceolatus venom for 24hr impaired respiratory control ratio and mitochondrial membrane permeability. Levels of mitochondrial DNA increased in the medium of cardiac cell preparation incubated with venom of Bothrops lanceolatus.

Our study suggests that whole venom of Bothrops lanceolatus impairs mitochondrial oxidative phosphorylation capacity and increases mitochondrial membrane permeability. Cardiac mitochondrial dysfunction associated with mitochondrial DAMPs release may alter myocardium function and engage the innate immune response, which may both participate to the cardiotoxicity occurring in patients with severe envenomation.

โ€ข Bioblast editor: Plangger M โ€ข O2k-Network Lab: FR Fort de France Neviere R


Labels: MiParea: Respiration, Pharmacology;toxicology 


Organism: Human  Tissue;cell: Heart  Preparation: Permeabilized tissue 


Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, CIV, NS, ROX  HRR: Oxygraph-2k 

2022-10