Cannon 2015 Fatty Acid Oxidation O2k-Network Discussion Forum

Cannon 2015 Fatty Acid Oxidation O2k-Network Discussion Forum

Link: O2k-Network Discussion Forum

Cannon DT (2015)

Event: Fatty Acid Oxidation O2k-Network Discussion Forum

• Saponin permeabilized skeletal muscle fiber bundles

• Protocol:

1. 0.5mM Malate
2. 50 µM palmitoyl-CoA + 2mM carnitine
3. 5mM ADP
4. From here on out, various combinations for titration protocol

• Coupling states:

1. LEAK_M+Palmitoylcarnitine
2. P_M+Palmitoylcarnitine
3. P_M+Palmitoylcarnitine+S
4. E_O+CCCP titrations
5. E_S+Rot
6. ROX_AntimycinA

• O2k-Network Lab: US CA San Diego Cannon DT

Labels:

Organism: Human, Mouse  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 

Coupling state: LEAK, OXPHOS, ET  Pathway: F, N, S, NS, ROX 

Palmitoylcarnitine