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Boada 2004 Free Radical Biol Med

From Bioblast
Publications in the MiPMap
Boada J, Cuesta E, Perales JC, Roig T, Bermudez J (2004) Glutathione content and adaptation to endogenously induced energy depletion in Mv1Lu cells. Free Radic Biol Med 15:1555-65.

Β» PMID: 15182857

Boada J, Cuesta E, Perales JC, Roig T, Bermudez J (2004) Free Radic Biol Med

Abstract: Transfection of genes that code for enzymes of energy metabolism provides alternative models to study the adaptive response to energy restriction induced by endogenous changes instead of by unfavorable environmental conditions. Overexpression of the glycolytic enzyme fructose-2,6-bisphosphatase reduced the content of fructose 2,6-bisphosphate, inducing energy limitation in the mink lung epithelial cell line Mv1Lu. This metabolic stress reduced the ATP available in transfected cells by 20%, which downregulated active ion transport and protein turnover. Ion homeostasis and cell function require concomitant reductions in cell membrane ion permeability and protein damage. Our results indicate that glutathione content linked these features of the adaptive response to the endogenously induced metabolic downregulation. β€’ Keywords: Metabolic stress, 6-Phosphofructo-2-kinase/fructose-2, 6-bisphosphatase, Fructose 2, 6-bisphosphate, Glutathione, Na+–K+ pump, Potassium channels, Protein synthesis, Free radicals

β€’ O2k-Network Lab: ES L'Hospitalet Bermudez MJ, ES Lleida Boada J


Labels:

Stress:Oxidative stress;RONS  Organism: Rat  Tissue;cell: Liver, Other cell lines  Preparation: Intact cells 



HRR: Oxygraph-2k