https://wiki.oroboros.at/api.php?action=feedcontributions&user=Bidaurratzaga+Eider&feedformat=atomBioblast - User contributions [en]2024-03-28T22:30:40ZUser contributionsMediaWiki 1.36.1https://wiki.oroboros.at/index.php?title=MitoGlobal_Events_2013&diff=55537MitoGlobal Events 20132013-12-13T08:35:53Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{Mainpage mitopedia}}<br />
[[Image:MitoGlobal.jpg|right|160px|link=http://www.bioblast.at/index.php/Mitochondrial_Global_Network|MitoGlobal]]<br />
== Mitochondrial Global Network Events 2013 ==<br />
<br />
'''MitoGlobal Events 2013''' - listed by '''[[Mitochondrial Global Network]]''' (MitoGlobal) ->> [[MitoGlobal Events 2014]] - >> [[MitoGlobal Events]]<br />
<br />
<br />
* '''Please inform us about upcoming events''' - send an e-mail to: [mailto:verena.laner@oroboros.at verena.laner@oroboros.at]<br />
<br />
<br />
{| class="wikitable" border="1"<br />
|-<br />
! scope="col" width="80px" | '''2013'''<br />
! scope="col" width="150px" | '''Link'''<br />
! scope="col" width="770px" | '''Event'''<br />
! scope="col" width="150px" | '''Location'''<br />
<br />
|-<br />
| '''December'''<br />
|-<br />
|<br />
: 18<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-workshops O2k-Workshop IOC83]<br />
| Pre-conference O2k-Workshop <br />
| Bangalore, India<br />
|-<br />
|<br />
: 19 to 20<br />
| [http://www.smrm2013.com/ SMRM2013]<br />
| Annual SMRM Conference, Society of Mitochondrial Research and Medicine, India<br />
| Bangalore, India<br />
|-<br />
|}<br />
<br />
<br />
'''Past MitoGlobal Events 2013'''<br />
<br />
<br />
{| class="wikitable" border="1"<br />
|-<br />
! scope="col" width="80px" | '''2013'''<br />
! scope="col" width="150px" | '''Link'''<br />
! scope="col" width="770px" | '''Event'''<br />
! scope="col" width="150px" | '''Location'''<br />
|-<br />
| '''December'''<br />
|-<br />
|<br />
: 05 to 06<br />
| [http://www.fertility-site.com/?utm_source=Sarbacane&utm_medium=email&utm_campaign=07%2F08%2F2013+Riga+-+7+aout Fertility & Antioxidants 2013]<br />
| 3rd World Congress on Fertility, Oxidative Stress & Antioxidants]]<br />
| Paris, France<br />
|-<br />
|<br />
: 09 to 11<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-workshops O2k-Workshop IOC82]<br />
| Pre-conference O2k-Workshop <br />
| Trondheim, Norway<br />
|-<br />
|<br />
: 11 to 13<br />
| [http://www.ntnu.edu/cerg/seminar-on-exercise-in-medicine Seminar on Exercise in Medicine]<br />
| 5th Seminar on Exercise in Medicine<br />
| Trondheim, Norway<br />
|-<br />
| '''November'''<br />
|-<br />
|<br />
: 02 to 03<br />
| [http://www.cug.ac.in/smrm.html Mitochondria in Health and Disease] <br />
| Mitochondria in Health and Disease <br />
| Gandhinagar, India<br />
|-<br />
|<br />
: 04 to 05<br />
| [http://www.asmrm2013.com/ ASMRM 2013] <br />
| 10<sup>th</sup> Conference of the Asian Society of Mitochondrial Research and Medicine ([[ASMRM]]) <br />
| Seoul, Korea<br />
|-<br />
|<br />
: 04<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-workshops O2k-Workshop IOC81]<br />
| ASMRM Pre-conference O2k-Workshop on [[High-Resolution Respirometry]]<br />
| Seoul, Korea<br />
|-<br />
|<br />
: 06 to 07<br />
| [http://mitochondria2013.org J-mit]<br />
| International Symposium on Mitochondria 2013 and the 13<sup>th</sup> Conference of Japanese Society of Mitochondrial Research and Medicine<br />
| Tokyo, Japan <br />
|-<br />
|<br />
: 07 to 09<br />
| [http://cepa2013.univ-lyon.fr CEPA 2013] <br />
| Colloque d'EcoPhysiology Animale] <br />
| Lyon, France<br />
|-<br />
|<br />
: 11 to 15<br />
| [http://researchnetworks.ki.se/converis/area/241 Course Karolinska Institute] <br />
| Course on Cancer ell energy metabolism<br />
| Stockolm, Norway<br />
|-<br />
|<br />
: 20 to 24<br />
| [http://www.sfrbm.org/sections/annual-meeting/20th-annual-meeting 20th SFRBM] <br />
| 20th Meeting of the Society for Free Radical Biology and Medicine (SFRBM) <br />
| San Antonio, TX, US<br />
|-<br />
| '''October'''<br />
|-<br />
|<br />
: 02 to 04<br />
| [https://registration.hinxton.wellcome.ac.uk/display_info.asp?id=349 WT Scientific conferences]<br />
| Mitochondrial Disease: Translating biology into new treatments<br />
| Cambridge, United Kingdom<br />
|-<br />
|<br />
: 09 to 14<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?IOC80_schroecken O2k-Fluorometry Workshop IOC80]<br />
| OROBOROS O2k-Fluorometry Workshop on [[High-Resolution Respirometry]] (HRR)<br />
| Schroecken, Austria<br />
|-<br />
|<br />
: 10 to 12<br />
| [http://events.embo.org/13-mac/ MAC'13]<br />
| Mitochondria, Apoptosis and Cancer 2013<br />
| Stockholm, Sweden<br />
|-<br />
|<br />
: 14 to 17<br />
| [http://www.oxyclubcalifornia.org/SFRBM_SA/index.php SFRBM-SA]<br />
| VIII Society Free Radical Biology and Medicine-South America <br />
| Buenos Aires, Argentinia<br />
|-<br />
|<br />
: 16 to 19<br />
| [http://oxyclubcalifornia.org/ICPH6/index.php ICPH6]<br />
| 6<sup>th</sup> International Conference on Polyphenols and Health<br />
| Buenos Aires, Argentina<br />
|-<br />
|<br />
: 17 to 18<br />
| [http://www.targeting-mitochondria.com/?utm_source=Sarbacane&utm_medium=email&utm_campaign=09%2F01%2F2013+Mitochondrie+-+At+your+diary+-+9+janvier Targeting Mitochondria]<br />
| 4<sup>th</sup> World Congress on Targeting Mitochondria <br />
| Berlin, Germany<br />
|-<br />
|<br />
: 24 to 25<br />
| [http://www.mobilehealth-site.com/newsletter.php?3&10272-267&15&o4A2vHpKvW8LBw%2F7AIoeAQ Mobilehealth]<br />
| Diabetes & Obesity World Congress<br />
| Riga, Latvia<br />
|-<br />
|<br />
: 25 to 26<br />
| [http://www.mito-medizin.de/fortbildung/ Curriculum 2013]<br />
| Modular course by IGRMM ''Fortbildung Klinische Mitochondrien-Medizin und Umweltmedizin - Kurs E''<br />
| Speyer, Germany<br />
|-<br />
|<br />
: 28 to Nov 01<br />
| [http://www.fbs.osaka-u.ac.jp/DynaMito2013/DynaMito2013/Welcome.html DynaMito2013]<br />
| 4 <sup>th</sup> International Symposium on Dynamics of Mitochondria (DynaMito 2013)<br />
| Okinawa, Japan<br />
|-<br />
| '''September'''<br />
|-<br />
|<br />
: 12 to 15<br />
| [http://oxidation2013.org.au/ Peroxidase Meeting 2013]<br />
| 8th International Peroxidase Meeting<br />
| Sydney, Australia<br />
|-<br />
|<br />
: 18 to 20<br />
| [http://www.uib.no/rg/mitochondrial_medicine/news/scandomit-2010 Scandomit]<br />
| 2013 ScandoMit conference<br />
| Oulu, Finland<br />
|-<br />
|<br />
: 19 to 23<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?IOC79_obergurgl O2k-Workshop IOC79]<br />
| OROBOROS O2k-Fluorometry-Workshop on [[High-Resolution Respirometry]] (HRR), MiP2013 pre-conference workshop. University of Innsbruck - Conference Centre Obergurgl.<br />
| Obergurgl, Austria<br />
|-<br />
|<br />
: 23 to 27<br />
| [[Image:MiPsocietyLOGO.JPG|left|90px|link=http://www.mitophysiology.org|MiPsociety]][http://www.mitophysiology.org/?MiP2013 MiP2013]<br />
| 9<sup>th</sup> MiP''conference'' - Comparative Mitochondrial Physiology: 10 years [[MiP]]''society'' - in honour of Kjell Johansen and Peter Hochachka. University of Innsbruck - Conference Centre Obergurgl.<br />
| Obergurgl, Austria<br />
|-<br />
|<br />
: 23 to 27<br />
| [http://www.easd2013.com/ EASD]<br />
| 49<sup>th</sup> EASD Annual Meeting<br />
|-<br />
|<br />
: 25 to 29<br />
| [http://gfbioenergetique.free.fr/Carry2013/indexCarry.html GFB]<br />
| 17<sup>th</sup> Congress of Bioenergetics French Group <br />
| Carry-le-Rouet, Marseille, France<br />
|-<br />
|<br />
: 27 to Sept 28<br />
| [http://www.mito-medizin.de/fortbildung/ Curriculum 2013]<br />
| Modular course by IGRMM ''Fortbildung Klinische Mitochondrien-Medizin und Umweltmedizin - Kurs D''<br />
| Speyer, Germany<br />
|-<br />
| '''August'''<br />
|-<br />
|<br />
: 26 to 30<br />
| [[Image:MiPsocietyLOGO.JPG|left|90px|link=http://www.mitophysiology.org|MiPsociety]][http://www.conferencemanager.dk/MiP2013/home.html MiP''summer'' 2013]<br />
| 6<sup>th</sup> MiP''summer'' School on Mitochondrial Physiology - University of Copenhagen<br />
| Copenhagen, Denmark<br />
|-<br />
| '''July'''<br />
|-<br />
|<br />
: 03 to 04<br />
| [http://www.mobilehealth-site.com/newsletter.php?3&10272-266&9&iiMAyAFbJdkGtRFWpz%2FyeA Oxidative Stress & Antioxidants 2013]<br />
| 12th International Conference on Oxidative Stress & Antioxidants<br />
| Paris, France<br />
|-<br />
| '''June'''<br />
|-<br />
|<br />
: 05 to 07<br />
| [http://oxyclubcalifornia.org/Paris2013/index.php SFRR Symposium]<br />
| French Society of Free Radical Research: 5<sup>th</sup> International Symposium: nutrition, oxygen biology and medicine<br />
| Paris, France<br />
|-<br />
|<br />
: 07 to 08<br />
| [http://www.ic2013.medicres.org/ medicReS Conference on Good Clinical Research]<br />
| International CME Conference on Good Clinical Research<br />
| Istanbul, Turkey<br />
|-<br />
|<br />
: 08 to 12<br />
| [http://www.hypoxia-finland.com/general-information/ Dealing with Hypoxia]<br />
| Oxygen 2013 conference of the [http://www.hypoxianet.com/about/organizers/ ''HypoxiaNet''] <br />
| Oulu, Finland<br />
|-<br />
|<br />
: 12 to 15<br />
| [http://www.umdf.org/site/c.piKYL1PHLtF/b.4864979/k.C003/Home.htm UMDF]<br />
| [[UMDF]] Conference - Mitochondrial Medicine<br />
| Newport Beach, CA, USA<br />
|-<br />
|<br />
: 13<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-workshops O2k-Workshop IOC77]<br />
| UMDF conference O2k-Workshop on [[High-Resolution Respirometry]]<br />
| Newport Beach, CA, USA<br />
|-<br />
|<br />
: 13 to 14<br />
| [http://www.mcc-studio.org/index.php?option=com_flexicontent&view=items&cid=46:2013&id=264:8th-forum-on-oxidative-stress-and-aging&Itemid=185 Forum on oxidative Stress]<br />
| International Forum on Oxidative Stress and Aging<br />
| Bologna, IT<br />
|-<br />
|<br />
: 16 to 21<br />
| [https://secure.faseb.org/FASEB/meetings/summrconf/Programs/11612.pdf FASEB ''Mitochondria'']<br />
| FASEB: Mitochondrial Biogenesis and Dynamics in Health Disease and Aging<br />
| Big Sky, Montana, USA<br />
|-<br />
|<br />
: 18 to 19<br />
| [[International Society on Oxygen Transport to Tissue|ISOTT]]<br />
| International Symposium on Metabolic Imaging and Spectroscopy Honoring the 100<sup>th</sup> Birthday of Britton Chance. University of Pennsylvania.<br />
| Philadelphia, USA<br />
|-<br />
|<br />
: 23 to 28<br />
| [http://www.grc.org/programs.aspx?year=2013&program=bioenerg GRC]<br />
| Gordon Research Conference on Bioenergetics ''Molecular Mechanisms and Fundamental Principles to Cellular Energetics in Health and Disease''<br />
| Proctor Academy, Andover, New Hampshire, USA<br />
|-<br />
|<br />
: 26 to 28<br />
| [http://www.isanh-me.com/?utm_source=Sarbacane&utm_medium=email&utm_campaign=29%2F04%2F2013+POlyphenols+-+29+avril International Conference on Antioxidants]<br />
| International Conference on Antioxidants<br />
| Marrakech, Morocco<br />
|-<br />
| '''May'''<br />
|-<br />
|<br />
: 04<br />
| [http://mitoaction.org/clinicalconference Mitoaction Clinical Conference]<br />
| 2013 Mitochondrial Disease Clinical Conference<br />
| Cambridge, MA, US<br />
|-<br />
|<br />
: 05 to 07<br />
| [http://www.cell-symposia-mitochondria.com/index.html Cell Symposia]<br />
| Symposium on Mitochondria: from Signaling to Disease<br />
| Lisbon, Portugal<br />
|-<br />
|<br />
: 06 to 07<br />
| [http://www.cvent.com/events/nhlbi-mitochondrial-biology-symposium-2013/event-summary-26f05e0e129748c8b816fafd1479f2de.aspx NHLBI2013]<br />
| 2013 NHLBI Mitochondrial Biology Symposium: Mitochondrial Genetics in Health and Disease<br />
| Bethesda, Maryland, USA<br />
|-<br />
|<br />
: 15 to 18<br />
| [http://lpi.oregonstate.edu/conf2013/index.html Diet and Optimum Health Conference]<br />
| Linus Pauling Institute: 7<sup>th</sup> conference on ''Diet and Optimum Health''<br />
| Oregon State University, Corvallis, Oregon, USA<br />
|-<br />
|<br />
: 26 to 31<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?IOC76_prague O2k-Workshop IOC76]<br />
| OROBOROS O2k-Workshop on [[High-Resolution Respirometry]] and O2k-Fluorometry<br />
| Prague, Czech Republic <br />
|-<br />
| '''April'''<br />
|-<br />
|<br />
: 10 to 13<br />
| [http://www.biochemistry.org/Conferences/AllConferences/tabid/379/Page/1/MeetingNo/SA150/view/Conference/Default.aspx 3<sup>rd</sup> Joint German/UK Bioenergetics Conference ]<br />
| 3<sup>rd</sup> D/UK Bioenergetics Conference on Bioenergetics (DUKBEC) 2013: Bioenergetics in mitochondria, bacteria and chloroplasts. Biochemical Society and Max Planck Institute of Biophysics.<br />
| Ebsdorfergrund, Germany<br />
|-<br />
|<br />
: 09 to 14<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?IOC75_schroecken O2k-Workshop IOC75]<br />
| OROBOROS O2k-Workshop on [[High-Resolution Respirometry]] (HRR)<br />
| Schroecken, Austria<br />
|-<br />
|<br />
: 14 to 19<br />
| [http://www.grc.org/programs.aspx?year=2013&program=oxidat GRC]<br />
| The 2013 Gordon Research Conference on OXIDATIVE STRESS AND DISEASE: The Metabolic – Inflammatory Axis in Brain Aging and Alzheimer’s Disease<br />
| Les Diablerets, Switzerland <br />
|-<br />
| <br />
: 17 to 20<br />
| [http://www.esci.eu.com/meetings/ ESCI2013] [http://www.esci.eu.com/wp-content/uploads/2012/11/ESCI-2013_Programme-ws-2_20-November-2012.doc Workshop-Programme]<br />
| 47<sup>th</sup> Annual Scientific Meeting of the European Society for Clinical Investigation incl. a two-day mitochondrial workshop entitled ''Mitochondrial physiology: from basic research to the clinic''<br />
| Albufeira, Portugal<br />
|-<br />
|<br />
: 24<br />
| [http://www.abcam.com/index.html?pageconfig=resource&rid=15718 UCL CfMR/Abcam]<br />
| UCL CfMR/Abcam mitochondrial symposium: ''Mitochondria, the cardiovascular system and metabolic syndrome''<br />
| Kennedy Lecture Theatre, Institute of Child Health, Guildford Street, London, UK <br />
|-<br />
|<br />
: 26 to 27<br />
| [http://www.mito-medizin.de IGRMM]<br />
| Curriculum MitoMedizin, Kurs B<br />
| Heidelberg, Germany<br />
|-<br />
<br />
|-<br />
| '''March'''<br />
|-<br />
|<br />
: 08 to Oct 26<br />
| [http://www.mito-medizin.de/fortbildung/ Curriculum 2013]<br />
| Modular course by IGRMM ''Fortbildung Klinische Mitochondrien-Medizin und Umweltmedizin''<br />
| Speyer, Germany<br />
|-<br />
|<br />
: 15 to 17<br />
| [http://www.bio.unipd.it/bam/ BAM]<br />
| Padova Muscle Days<br />
| Padova, Italy<br />
|-<br />
|-<br />
| '''February'''<br />
|-<br />
|<br />
: 13 to 16<br />
| [http://www.zingconferences.com/index.cfm?page=conference&intConferenceID=103 Zing Aging Conference]<br />
| Mitochondria, metabolic regulation and the Biology of Aging Conference 2013<br />
| Lanzarote, Spain<br />
|-<br />
|<br />
: 18 to 21<br />
| [[UCL Consortium for Mitochondrial Research]]<br />
| Joint Anglo-Israeli Mitochondrial Biology Workshop, Weizmann Institute<br />
| Rehovot, Israel<br />
|-<br />
|<br />
: 24 to Mar 03<br />
| [http://www.neopterin.net/ Neopterin]<br />
| 32<sup>nd</sup> International Winter-Workshop on Clinical, Chemical and Biochemical Aspects of Pteridines<br />
| St. Christoph, Arlberg, Austria<br />
|-<br />
|<br />
: 26 to Mar 02<br />
| [http://www.hypoxia.net/index.htm Hypoxia]<br />
| Hypoxia XVIII. International Hypoxia Symposia<br />
| Lake Louise, Alberta, Canada<br />
|-<br />
| '''January'''<br />
|-<br />
|<br />
: 10 to 12<br />
| [http://eci2013.manipal.edu/ ECI2013]<br />
| Epigenomics and Cancer Informatics - Symposium and Workshop<br />
| Manipal, India<br />
|-<br />
|<br />
: 13 to 17<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?IOC73_kalkota O2k-Workshop IOC73] <br />
| Neurocon Pre-Conference Workshop on Recent Techniques in Neuroscience Research, HRR and OROBOROS Oxygraph-2k<br />
| Kolkata, India<br />
|-<br />
|<br />
: 18 to 20<br />
| [https://sites.google.com/site/neuroconkolkata/ Neurocon 2013]<br />
| International Conference on Neurodegenerative and Neurodevelopmental Disorders: Translational Aspects<br />
| Kolkata, India<br />
|-<br />
|<br />
: 21 to 27<br />
| [http://www.eblab.cl/wp-content/uploads/2011/09/Poster-v3.pdf Unveiling Mitochondria 2.0]<br />
| Unveiling Mitochondria 2.0: Hands on Training in Live Cell Imaging and Respirometry<br />
| Santiago, Chile<br />
|-<br />
|<br />
: 22 to 25<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?IOC74_innsbruck IOC74]<br />
| International O2k-Workshop on [[High-Resolution Respirometry]] (HRR) - TPP/ISE<br />
| Innsbruck, Austria<br />
|-<br />
|<br />
: 25 to 26<br />
| [http://www.klinikum.uni-heidelberg.de/High-Altitude-Tolerance.128939.0.html HAT2013]<br />
| Symposium ''High Altitude Tolerance''<br />
| Heidelberg, Germany<br />
|}<br />
<br />
<br />
<br />
<br />
<br />
[[Category:Mitochondrial Global Network]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=Liesa-Roig_M&diff=55536Liesa-Roig M2013-12-12T15:17:55Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{Person<br />
|lastname=Liesa-Roig<br />
|firstname=Marc<br />
|title=Postdoctoral Associate<br />
<br />
<br />
|institution=Boston University School of Medicine<br />
Biochemistry Department<br />
<br />
|address=650 Albany Street<br />
|area code=02118<br />
|city=Boston<br />
|state=Massachusetts (MA)<br />
|country=USA<br />
|mailaddress=liesa@bu.edu<br />
}}<br />
{{Labelingperson}}</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=ES_Barcelona_Zorzano_A&diff=55535ES Barcelona Zorzano A2013-12-12T15:15:57Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{MiPNetLab<br />
|institution=Institute for Research in Biomedicine (IRB Barcelona)<br />
<br />
Parc Cientific de Barcelona &<br />
<br />
Departament de Bioquímica i Biologia Molecular<br />
<br />
Facultat de Biologia - Universitat de Barcelona<br />
|address=<br />
c/ Baldiri Reixac, 10-12<br />
|area code=08028<br />
|city=Barcelona<br />
|country=Spain<br />
|Contact=Zorzano A<br />
|MiPNetLab=Hernandez-Alvarez MI, Lopes Ramos X, Molner N<br />
|Team previous=Segales J, Liesa-Roig M<br />
|Status=[http://www.oroboros.at/?Power-O2k 2 Power-O2k] 2008-<br />
|info=[http://www.oroboros.at/?IOC70 IOC70], [http://www.oroboros.at/?ioc46-schroecken IOC46]<br />
|Info=[http://www.oroboros.at/index.php?id=1229 ES_Barcelona_ZorzanoA]<br />
}}<br />
<br />
<br />
* [[Talk:MiPNet_Reference_Laboratories#MiPNet Reference Laboratories - highlighted|MiPNet Reference Laboratory of the Week - 2012-05-16]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=ES_L%27Hospitalet_Bermudez_MJ&diff=55534ES L'Hospitalet Bermudez MJ2013-12-12T15:15:21Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{MiPNetLab<br />
|institution=Departament de Ciències Fisiològiques II<br />
<br />
Divisió de Ciències de la Salut<br />
<br />
Universitat de Barcelona<br />
|address=Feixa Llarga s/n<br />
<br />
L'Hospitalet de Llobregat<br />
|area code=08907<br />
|city=Barcelona<br />
|country=Spain<br />
|Contact=Bermudez MJ<br />
|MiPNetLab=Cuesta E, Lopez RJ, Pallares R, Perales JC, <br />
|Team previous=Roig RT, Blasco Sole FX<br />
|Status=O2k 2003-<br />
|info=[http://www.oroboros.at/?IOC70 IOC70], [http://www.oroboros.at/index.php?id=previouscourses IOC29], [http://www.oroboros.at/index.php?id=previouscourses IOC24]<br />
|keyword=Liver cells<br />
|keywords=Liver cells<br />
|Info=[http://www.oroboros.at/index.php?id=bermudez_j ES_Barcelona_BermudezMJ]<br />
}}<br />
* [[Talk:MiPNet_Reference_Laboratories#MiPNet Reference Laboratories - highlighted|MiPNet Reference Laboratory of the Week - 2012-05-16]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=ES_L%27Hospitalet_Bermudez_MJ&diff=55533ES L'Hospitalet Bermudez MJ2013-12-12T13:37:39Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{MiPNetLab<br />
|institution=Departament de Ciències Fisiològiques II<br />
<br />
Divisió de Ciències de la Salut<br />
<br />
Universitat de Barcelona<br />
|address=Feixa Llarga s/n<br />
<br />
L'Hospitalet de Llobregat<br />
|area code=08907<br />
|city=Barcelona<br />
|country=Spain<br />
|Contact=Bermudez MJ<br />
|MiPNetLab=Blasco Sole FX, Cuesta E, Lopez RJ, Pallares R, Perales JC, <br />
|Team previous=Roig RT<br />
|Status=O2k 2003-<br />
|info=[http://www.oroboros.at/?IOC70 IOC70], [http://www.oroboros.at/index.php?id=previouscourses IOC29], [http://www.oroboros.at/index.php?id=previouscourses IOC24]<br />
|keyword=Liver cells<br />
|keywords=Liver cells<br />
|Info=[http://www.oroboros.at/index.php?id=bermudez_j ES_Barcelona_BermudezMJ]<br />
}}<br />
* [[Talk:MiPNet_Reference_Laboratories#MiPNet Reference Laboratories - highlighted|MiPNet Reference Laboratory of the Week - 2012-05-16]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=Heidler_Juliana&diff=55474Heidler Juliana2013-12-10T14:02:12Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{Person<br />
|lastname=Heidler<br />
|firstname=Juliana<br />
|title=PhD<br />
|institution=[[File:Heidler.jpg|center|150px|Juliana Heidler]]<br />
OROBOROS Instruments<br />
|address=Schöpfstraße 18<br />
|area code=6020<br />
|city=Innsbruck<br />
|country=Austria<br />
|mailaddress=juliana.heidler@oroboros.at<br />
}}<br />
{{Labelingperson<br />
|field of research=Basic<br />
}}<br />
==Participated at==<br />
*[[MiP2013]]<br />
* [[IOC79| O2k-Workshop IOC79]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=File:Heidler.jpg&diff=55473File:Heidler.jpg2013-12-10T14:01:46Z<p>Bidaurratzaga Eider: uploaded a new version of &quot;File:Heidler.jpg&quot;</p>
<hr />
<div></div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=DK_Copenhagen_Poulsen_HE&diff=55448DK Copenhagen Poulsen HE2013-12-09T10:45:00Z<p>Bidaurratzaga Eider: </p>
<hr />
<div><nowiki><br />
{{MiPNetLab<br />
|institution=Centre of Diagnostic Investigation <br />
Dept. of Clinical Pharmacology Q<br />
Copenhagen University Hospital<br />
<br />
<br />
<br />
<br />
|address=20 Tagensvej, Section 7642<br />
|area code=2200 <br />
|city=Copenhagen <br />
|country=Denmark<br />
|Contact=Poulsen HE<br />
|MiPNetLab=Kjaer Hansen L<br />
|info=IOC<br />
|Team=<br />
}}</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=DK_Copenhagen_Poulsen_HE&diff=55447DK Copenhagen Poulsen HE2013-12-09T10:44:42Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{MiPNetLab<br />
|institution=Centre of Diagnostic Investigation <br />
Dept. of Clinical Pharmacology Q<br />
Copenhagen University Hospital<br />
<br />
<br />
<br />
<br />
|address=20 Tagensvej, Section 7642<br />
|area code=2200 <br />
|city=Copenhagen <br />
|country=Denmark<br />
|Contact=Poulsen HE<br />
|MiPNetLab=Kjaer Hansen L<br />
|info=IOC<br />
|Team=<br />
}}</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=DK_Copenhagen_Poulsen_HE&diff=55446DK Copenhagen Poulsen HE2013-12-09T10:40:38Z<p>Bidaurratzaga Eider: Created page with " {{MiPNetLab |institution=Comparative Exercise Physiology Laboratory Center for Veterinary Health Sciences Oklahoma State University |address=264 McElroy Hall |area code=74078 ..."</p>
<hr />
<div><br />
{{MiPNetLab<br />
|institution=Comparative Exercise Physiology Laboratory<br />
Center for Veterinary Health Sciences<br />
Oklahoma State University<br />
<br />
<br />
|address=264 McElroy Hall<br />
|area code=74078<br />
|city=Stillwater<br />
|state=Oklahoma (OK)<br />
|country=USA<br />
|weblink=[http://www.cvm.okstate.edu/ Oklahoma state university]<br />
|Contact=Davis MS<br />
|Team=<br />
}}</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=Kjaer_Hansen_L&diff=55445Kjaer Hansen L2013-12-09T10:35:10Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{Person<br />
|lastname=Kjaer Hansen<br />
|firstname=Lis<br />
|institution=Clinical Pharmacology<br />
<br />
|address=Blegdamsvej 9<br />
Q7642<br />
<br />
<br />
|area code=2200 <br />
|city=Copenhagen<br />
|country=Denmark<br />
|mailaddress=lkjaer@rh.regionh.dk<br />
}}<br />
{{Labelingperson}}</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=Kjaer_Hansen_L&diff=55444Kjaer Hansen L2013-12-09T10:32:22Z<p>Bidaurratzaga Eider: Created page with "{{Person |lastname=Kjaer Hansen |firstname=Lis |title= |institution= |address= |area code= |city= |country=Austria |mailaddress=christiane.bauer@oroboros.at }} {{Labelingperson}}"</p>
<hr />
<div>{{Person<br />
|lastname=Kjaer Hansen<br />
|firstname=Lis<br />
|title=<br />
|institution=<br />
<br />
|address=<br />
|area code=<br />
|city=<br />
|country=Austria<br />
|mailaddress=christiane.bauer@oroboros.at<br />
}}<br />
{{Labelingperson}}</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=AT_Innsbruck_Oroboros&diff=55443AT Innsbruck Oroboros2013-12-09T10:12:22Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{MiPNetLab<br />
|institution=OROBOROS INSTRUMENTS Corp<br />
<br />
[[high-resolution respirometry]]<br />
|address=Schöpfstr. 18<br />
<br />
See also [[AT_Innsbruck_Gnaiger E]]<br />
|area code=A-6020<br />
|city=Innsbruck<br />
|country=Austria<br />
|weblink=http://www.oroboros.at<br />
|Contact=Gnaiger E<br />
|MiPNetLab=Bauer CM, Capek O, Fasching M, Fontana-Ayoub M, Gradl L, Gradl P, Harrison DK, Heidler J, Krumschnabel G, Laner V, Marte V, Piper F<br />
|Team previous=Kuznetsov AV, Pesta D, Sumbalova Z<br />
|Status=[http://www.oroboros.at/?Power-O2k '''The Power-O2k'''] 1992-<br />
|info=[[Bioblast 2012]], [http://www.oroboros.at/?O2k-workshops O2k-Workshops]<br />
|keyword=[[high-resolution respirometry]]<br />
|Field_of_interest=[Oxygraph-2k], [high-resolution respirometry], [MitoCom Tyrol]<br />
|prevcollab=Steinlechner-Maran R, Lassnig B, Garedew A, Lemieux H, Scandurra F<br />
|Info=[http://www.oroboros.at]<br />
}}<br />
== The O2k-Team ==<br />
<br />
* The [[O2k-Team]] is presented here in terms of the staff of OROBOROS INSTRUMENTS, collaborators in the [[AT_Innsbruck_Gnaiger E|Mitochondrial Physiology Research Group]] of Erich Gnaiger at the Medical University of Innsbruck, and core company partners.<br />
* [[Gradl P|Philipp Gradl]] is the CEO of an independent company partner (WGT-Elektronik) of the O2k-Team, cooperating in the development of the O2k since 2001, responsible for the electronics, mechanics, and production.<br />
* [[Gradl L|Lukas Gradl]] is an independent company partner of the O2k-Team, cooperating in the development of [[DatLab]] since 2001.<br />
<br />
<br />
== ''MitoCom Tyrol'' ==<br />
* [[MitoCom_Partners|Partner of K-Regio ''MitoCom Tyrol'']]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=Bauer_CM&diff=55442Bauer CM2013-12-09T10:11:18Z<p>Bidaurratzaga Eider: Created page with "{{Person |lastname=Bauer |firstname=Christiane Maria |title=PhD |institution= OROBOROS INSTRUMENTS Corp. high-resolution respirometry |address=Schöpfstr. 18 |area code=A-6020 ..."</p>
<hr />
<div>{{Person<br />
|lastname=Bauer<br />
|firstname=Christiane Maria<br />
|title=PhD<br />
|institution=<br />
<br />
OROBOROS INSTRUMENTS Corp.<br />
<br />
high-resolution respirometry<br />
|address=Schöpfstr. 18<br />
|area code=A-6020<br />
|city=Innsbruck<br />
|country=Austria<br />
|mailaddress=christiane.bauer@oroboros.at<br />
}}<br />
{{Labelingperson}}</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=Legkun_G&diff=55441Legkun G2013-12-09T10:06:45Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{Person<br />
|lastname=Legkun<br />
|firstname=German<br />
|title=<br />
|institution=Department of General and Applied Physics<br />
Moscow Institute of Physics and Technology<br />
<br />
|area code=<br />
|city=Dolgoprudny<br />
|country=Russia<br />
|mailaddress=legkun.german@gmail.com<br />
}}<br />
<br />
{{Labelingperson}}<br />
== Participated at ==<br />
* IOC Schroecken April-2014</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=Legkun_G&diff=55440Legkun G2013-12-09T10:04:19Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{Person<br />
|lastname=Legkun<br />
|firstname=German<br />
|institution=Department of General and Applied Physics<br />
Moscow Institute of Physics and Technology<br />
<br />
|city=Dolgoprudny<br />
|country=Russia<br />
|mailaddress=legkun.german@gmail.com<br />
|Institution=<br />
|Area_Code=<br />
|City=<br />
|State_/_Province=<br />
|Country_name=<br />
|Weblink=<br />
}}<br />
{{Labelingperson|Institution=<br />
|Area_Code=<br />
|City=<br />
|State_/_Province=<br />
|Country_name=<br />
|Weblink=<br />
}}</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=Legkun_G&diff=55439Legkun G2013-12-09T10:01:31Z<p>Bidaurratzaga Eider: Created page with "{{Person |lastname= |firstname= |title= |Institution= |address= |Area Code= |City= |State / Province= |Country name= |mailaddress= |Weblink= }}"</p>
<hr />
<div>{{Person<br />
|lastname=<br />
|firstname=<br />
|title=<br />
|Institution=<br />
|address=<br />
|Area Code=<br />
|City=<br />
|State / Province=<br />
|Country name=<br />
|mailaddress=<br />
|Weblink=<br />
}}</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet09.12_O2k-Titrations&diff=55438MiPNet09.12 O2k-Titrations2013-12-09T09:08:42Z<p>Bidaurratzaga Eider: /* Further information */</p>
<hr />
<div>{{Publication<br />
|title=OROBOROS (2013) Oxygraph-2k titrations: Mitochondria, permeabilized cells and biopsies. Mitochondr Physiol Network 09.12. Version 9.<br />
|info=[[Media:MiPNet09.12 O2k-Titrations.pdf|'''Open Access-MiPNet09.12.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet09.12_O2k-Titrations.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=2004<br />
|journal=Mitochondr Physiol Network<br />
|abstract='''O2k-Protocol for Mitochondria, Permeabilized Cells, and Biopsies'''<br />
<br />
A list of substrates, uncouplers, and inhibitors used in [[SUIT]] protocols in [[high-resolution respirometry]], with information on abbreviations, stock concentrations, final experimental concentrations, volumes titrated into the 2 ml O2k-chamber, and syringes used for manual titrations.<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
{{Labeling<br />
|area=Instruments;methods<br />
|topics=ATP; ADP; AMP; PCr, Substrate; Glucose; TCA Cycle, Amino Acid<br />
|instruments=Protocol<br />
|additional=Chemicals & Media<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
== Further information ==<br />
:>> [http://www.oroboros.at/?protocols_titrations Excel Templates and DatLab-Demo Files]<br />
* Version 8: [[Gnaiger 2012 MitoPathways]]<br />
* Version 1: 2004<br />
<br />
<br />
[[Category:OroboPedia]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=O2k-Publications_2012&diff=55422O2k-Publications 20122013-12-05T13:11:49Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>[[File:O2k-Publications.png|left|100px|caption]]<br />
'''[[O2k-Publications|Chronological Reference List]]''' / [[O2k-Publications 2013|2013]], [[O2k-Publications 2012|2012]], [[O2k-Publications 2011|2011]], [[O2k-Publications 2010|2010]], [[O2k-Publications 2009|2009]], [[O2k-Publications 2008|2008]], [[O2k-Publications 2007|2007]], [[O2k-Publications 2006|2006]], [[O2k-Publications 2005|2005]], [[O2k-Publications 2004|2004]], [[O2k-Publications 2003|2003]], [[O2k-Publications 2002|2002]], [[O2k-Publications 2001|2001]], [[O2k-Publications 2000|2000]], [[O2k-Publications 1999|1999]], [[O2k-Publications 1998|1998]], [[O2k-Publications 1997|1997]], [[O2k-Publications 1996|1996]], [[O2k-Publications 1995|1995]], [[O2k-Publications 1994|1994]], [[O2k-Publications 1993|1993]]<br />
<br />
'''Number of Publications in year 2012: '''{{#ask:[[Category:Publications]][[Was published in year::2012]]<br />
|format=count<br />
}}<br />
<br />
{{#ask:[[Category:Publications]][[Was published in year::2012]][[Instrument and method::Oxygraph-2k]] <br />
|?Has title=Reference<br />
|?Tissue and cell<br />
|?Cell line<br />
|?Mammal and model<br />
|?Nonmammal<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was written by<br />
|order=ascending<br />
}}<br />
<br />
[[Category:Publications]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=O2k-Publications_2012&diff=55421O2k-Publications 20122013-12-05T13:11:39Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>[[File:O2k-Publications.png|left|100px|caption]]<br />
'''[[O2k-Publications|Chronological Reference List]]''' / [[O2k-Publications 2013|2013]], [[O2k-Publications 2012|2012]], [[O2k-Publications 2011|2011]], [[O2k-Publications 2010|2010]], [[O2k-Publications 2009|2009]], [[O2k-Publications 2008|2008]], [[O2k-Publications 2007|2007]], [[O2k-Publications 2006|2006]], [[O2k-Publications 2005|2005]], [[O2k-Publications 2004|2004]], [[O2k-Publications 2003|2003]], [[O2k-Publications 2002|2002]], [[O2k-Publications 2001|2001]], [[O2k-Publications 2000|2000]], [[O2k-Publications 1999|1999]], [[O2k-Publications 1998|1998]], [[O2k-Publications 1997|1997]], [[O2k-Publications 1996|1996]], [[O2k-Publications 1995|1995]], [[O2k-Publications 1994|1994]], [[O2k-Publications 1993|1993]]<br />
<br />
'''Number of Publications in year 2012: '''{{#ask:[[Category:Publications]][[Was published in year::2013]]<br />
|format=count<br />
}}<br />
<br />
{{#ask:[[Category:Publications]][[Was published in year::2012]][[Instrument and method::Oxygraph-2k]] <br />
|?Has title=Reference<br />
|?Tissue and cell<br />
|?Cell line<br />
|?Mammal and model<br />
|?Nonmammal<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was written by<br />
|order=ascending<br />
}}<br />
<br />
[[Category:Publications]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=O2k-Publications_2012&diff=55420O2k-Publications 20122013-12-05T13:11:27Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>[[File:O2k-Publications.png|left|100px|caption]]<br />
'''[[O2k-Publications|Chronological Reference List]]''' / [[O2k-Publications 2013|2013]], [[O2k-Publications 2012|2012]], [[O2k-Publications 2011|2011]], [[O2k-Publications 2010|2010]], [[O2k-Publications 2009|2009]], [[O2k-Publications 2008|2008]], [[O2k-Publications 2007|2007]], [[O2k-Publications 2006|2006]], [[O2k-Publications 2005|2005]], [[O2k-Publications 2004|2004]], [[O2k-Publications 2003|2003]], [[O2k-Publications 2002|2002]], [[O2k-Publications 2001|2001]], [[O2k-Publications 2000|2000]], [[O2k-Publications 1999|1999]], [[O2k-Publications 1998|1998]], [[O2k-Publications 1997|1997]], [[O2k-Publications 1996|1996]], [[O2k-Publications 1995|1995]], [[O2k-Publications 1994|1994]], [[O2k-Publications 1993|1993]]<br />
<br />
'''Number of Publications in year 2012: '''{{#ask:[[Category:Publications]][[Was published in year::2013]]<br />
|format=count<br />
}}<br />
<br />
{{#ask:[[Category:Publications]][[Was published in year::2012]][[Instrument and method::Oxygraph-2k]] <br />
|?Has title=Reference<br />
|?Tissue and cell<br />
|?Cell line<br />
|?Mammal and model<br />
|?Nonmammal<br />
|format=broadtable<br />
|limit=5000<br />
|offset=0<br />
|sort=Was written by<br />
|order=ascending<br />
}}<br />
<br />
[[Category:Publications]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=O2k-Publications_2013&diff=55419O2k-Publications 20132013-12-05T13:07:04Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>[[File:O2k-Publications.png|left|100px|caption]]<br />
'''[[O2k-Publications|Chronological Reference List]]''' / [[O2k-Publications 2013|2013]], [[O2k-Publications 2012|2012]], [[O2k-Publications 2011|2011]], [[O2k-Publications 2010|2010]], [[O2k-Publications 2009|2009]], [[O2k-Publications 2008|2008]], [[O2k-Publications 2007|2007]], [[O2k-Publications 2006|2006]], [[O2k-Publications 2005|2005]], [[O2k-Publications 2004|2004]], [[O2k-Publications 2003|2003]], [[O2k-Publications 2002|2002]], [[O2k-Publications 2001|2001]], [[O2k-Publications 2000|2000]], [[O2k-Publications 1999|1999]], [[O2k-Publications 1998|1998]], [[O2k-Publications 1997|1997]], [[O2k-Publications 1996|1996]], [[O2k-Publications 1995|1995]], [[O2k-Publications 1994|1994]], [[O2k-Publications 1993|1993]]<br />
<br />
'''Number of Publications in year 2013: '''{{#ask:[[Category:Publications]][[Was published in year::2013]]<br />
|format=count<br />
}}<br />
<br />
{{#ask:[[Category:Publications]][[Was published in year::2013]][[Instrument and method::Oxygraph-2k]] <br />
|?Has title=Reference <br />
|?Tissue and cell <br />
|?Cell line <br />
|?Mammal and model <br />
|?Nonmammal <br />
|index=all<br />
|format=broadtable<br />
|index=true<br />
|limit=5000<br />
|offset=0<br />
|sort=Was written by<br />
|order=ascending<br />
}}<br />
<br />
[[Category:Publications]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MitoGlobal_Events_2014&diff=55352MitoGlobal Events 20142013-12-03T08:13:30Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>= Mitochondrial Global Network Events 2014 =<br />
[[Image:MitoGlobal.jpg|right|160px|link=http://www.bioblast.at/index.php/Mitochondrial_Global_Network|MitoGlobal]]<br />
<br />
<br />
<br />
'''MitoGlobal Events 2014''' - listed by '''[[Mitochondrial Global Network]]''' ->> [[MitoGlobal Events 2015]] - >> [[MitoGlobal Events]]<br />
<br />
<br />
* '''Please inform us about upcoming mitochondria events''' --> send an e-mail to: [mailto:verena.laner@oroboros.at verena.laner@oroboros.at]<br />
<br />
<br />
<br />
<br />
<br />
{| class="wikitable" border="1"<br />
|-<br />
! scope="col" width="80px" | '''2014'''<br />
! scope="col" width="150px" | '''Link'''<br />
! scope="col" width="770px" | '''Event'''<br />
! scope="col" width="150px" | '''Location'''<br />
|-<br />
| '''January'''<br />
|-<br />
|<br />
: <br />
| <br />
| <br />
| <br />
|-<br />
| '''February'''<br />
|-<br />
|<br />
: 09 to 14<br />
| [http://www.grc.org/programs.aspx?year=2014&program=oxygenrad GRC]<br />
| Oxygen Radicals Gordon Research Conference<br />
| Ventura, CA, US <br />
|-<br />
|<br />
: 18 to 23<br />
| [http://www.keystonesymposia.org/index.cfm?e=web.meeting.program&meetingid=1266 Keystone Symposia]<br />
| Mitochondrial Dynamics and Physiology (Q5) joint with the meeting on The Chemistry and Biology of Cell Death (Q6)<br />
| Santa Fe, New Mexico, US <br />
|-<br />
| '''March'''<br />
|-<br />
| '''April'''<br />
|-<br />
|<br />
: 02 April <br />
|[http://sigs.nih.gov/mito/Pages/default.aspx Mitochondrial Interest Group]<br />
|A Mitochondrial Etiology of Metabolic and Degenerative Diseases Cancer and Ageing - lecture by Dr. Wallace and Transformational Medicine in the Mitochondrial Age Symposium<br />
| Bethesda, US<br />
|-<br />
|<br />
: 07 to 12<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-workshops O2k-Fluorometry Workshop IOC]<br />
| OROBOROS O2k-Workshop on [[High-Resolution Respirometry]] (HRR)<br />
| Schroecken, AT<br />
|-<br />
|<br />
: 26 to 30<br />
| <br />
| Symposium for Experimental Biology<br />
| San Diego, CA, US<br />
|-<br />
|<br />
:30 April to 03 May<br />
| [http://www.esci.eu.com/meetings/ ESCI]<br />
| European Society for Clinical Investigation<br />
| Utrecht, NL<br />
|-<br />
| '''May'''<br />
|-<br />
|<br />
:04 to 07<br />
| [http://meetochondrie.ibgc.cnrs.fr/ Meet''O''chondrie 2014]<br />
| 7<sup>th</sup> Colloque Meet''O''chondrie<br />
| Evian, Haute-Savoie, FR<br />
|-<br />
|<br />
: 07 to 10<br />
| [http://oxyclubcalifornia.org/OCC2014/index.php OCC2014]<br />
| Oxygen Club of California 2014 World Congress<br />
| Davis, CA, US<br />
|-<br />
|<br />
: 14 to 16<br />
| [http://hq.prosite.be:85/NICC/2013pre/dna-forensics-2014 DNA Forensics 2014]<br />
| 9<sup>th</sup> International '''Y-chromosome workshop''' & 6<sup>th</sup> International '''EMPOP meeting'''<br />
| Brussels, BE<br />
|-<br />
|<br />
: 25 to 31<br />
| [http://www.ismm2014.org/Pages/default.aspx ISMM2014]<br />
| World Congress on High Altitude Medicine and Physiology<br />
| Bolzano, IT<br />
|-<br />
| '''June'''<br />
|-<br />
|<br />
: 04 to 07<br />
| [http://www.umdf.org/site/c.piKYL1PHLtF/b.4864979/k.C003/Home.htm UMDF2014]<br />
| Mitochondrial Medicine 2014<br />
| Pittsburgh, PA, US<br />
|-<br />
|<br />
: 15 to 19<br />
| [[Image:Euromit2014.jpg|100px]][http://www.euromit2014.org/ EUROMIT 2014]<br />
| EUROMIT 2014 - International Meeting on Mitochondrial Pathology<br />
| Tampere, FI<br />
|-<br />
|<br />
:28 to 3 July<br />
| [http://www.isott2014.org/ ISOTT 2014]<br />
| 42nd Meeting of the International Society on Oxygen Transport to Tissue<br />
| London, UK<br />
|-<br />
| '''July'''<br />
|-<br />
|<br />
: 06 to 10<br />
| [http://www.respiratory-science.org/ International Congress of Respiratory Science]<br />
| 3rd International Congress of Respiratory Science<br />
| Bad Honnef, DE<br />
|-<br />
|<br />
: <br />
| [[Image:MiPsocietyLOGO.JPG|left|90px|link=http://www.mitophysiology.org|MiPsociety]][http://www.mitophysiology.org/?home_mip '''MiP''summer'' 2014''']<br />
| 7<sup>th</sup> MiP''summer'' School on Mitochondrial Physiology - University College of London<br />
| London, UK<br />
|-<br />
|<br />
: 12 to 17<br />
| [http://www.ebec2014.org/ EBEC2014]<br />
| 18th European Bioenergetics Conference - EBEC 2014<br />
| Lisbon, PT<br />
|-<br />
| '''August'''<br />
|-<br />
|<br />
: <br />
| <br />
| <br />
| <br />
|-<br />
| '''September'''<br />
|-<br />
|<br />
: 08 to 12<br />
| [[Image:MiPsocietyLOGO.JPG|left|90px|link=http://www.mitophysiology.org|MiPsociety]]'''[[MiP2014]]''' <br />
| [[Image:IUBMB-logo.gif|left|60px|link=http://www.iubmb.org|IUBMB - ''International Union of Biochemistry and Molecular Biology'']]10<sup>th</sup> MiP''conference'': Joint IUBMB/MiP Symposium on Mitochondrial Physiology - a Point/Counterpoint Meeting <br />
| Obergurgl, AT<br />
|-<br />
|<br />
: 12 to 16<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-Workshops O2k-Fluorometry Workshop IOC]<br />
| OROBOROS post-conference Workshop<br />
| Obergurgl, AT<br />
|-<br />
| '''October'''<br />
|-<br />
|<br />
: <br />
| <br />
| <br />
| <br />
|-<br />
| '''November'''<br />
|-<br />
|<br />
: <br />
| <br />
| <br />
| <br />
|-<br />
| '''December'''<br />
|-<br />
|<br />
: <br />
| <br />
| <br />
| <br />
|-<br />
|-<br />
|}<br />
<br />
<br />
[[Category:Mitochondrial Global Network]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=File:Euromit2014.jpg&diff=55351File:Euromit2014.jpg2013-12-03T08:13:16Z<p>Bidaurratzaga Eider: </p>
<hr />
<div></div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MitoGlobal_Events_2013&diff=55350MitoGlobal Events 20132013-12-03T07:21:15Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{Mainpage mitopedia}}<br />
[[Image:MitoGlobal.jpg|right|160px|link=http://www.bioblast.at/index.php/Mitochondrial_Global_Network|MitoGlobal]]<br />
== Mitochondrial Global Network Events 2013 ==<br />
<br />
'''MitoGlobal Events 2013''' - listed by '''[[Mitochondrial Global Network]]''' (MitoGlobal) ->> [[MitoGlobal Events 2014]] - >> [[MitoGlobal Events]]<br />
<br />
<br />
* '''Please inform us about upcoming events''' - send an e-mail to: [mailto:verena.laner@oroboros.at verena.laner@oroboros.at]<br />
<br />
<br />
{| class="wikitable" border="1"<br />
|-<br />
! scope="col" width="80px" | '''2013'''<br />
! scope="col" width="150px" | '''Link'''<br />
! scope="col" width="770px" | '''Event'''<br />
! scope="col" width="150px" | '''Location'''<br />
<br />
|-<br />
| '''December'''<br />
|-<br />
|<br />
: 05 to 06<br />
| [http://www.fertility-site.com/?utm_source=Sarbacane&utm_medium=email&utm_campaign=07%2F08%2F2013+Riga+-+7+aout Fertility & Antioxidants 2013]<br />
| 3rd World Congress on Fertility, Oxidative Stress & Antioxidants]]<br />
| Paris, France<br />
|-<br />
|<br />
: 19 to 20<br />
| [http://www.smrm2013.com/ SMRM2013]<br />
| Annual SMRM Conference, Society of Mitochondrial Research and Medicine, India<br />
| Bangalore, India<br />
|-<br />
|}<br />
<br />
<br />
'''Past MitoGlobal Events 2013'''<br />
<br />
<br />
{| class="wikitable" border="1"<br />
|-<br />
! scope="col" width="80px" | '''2013'''<br />
! scope="col" width="150px" | '''Link'''<br />
! scope="col" width="770px" | '''Event'''<br />
! scope="col" width="150px" | '''Location'''<br />
|-<br />
| '''November'''<br />
|-<br />
|<br />
: 02 to 03<br />
| [http://www.cug.ac.in/smrm.html Mitochondria in Health and Disease] <br />
| Mitochondria in Health and Disease <br />
| Gandhinagar, India<br />
|-<br />
|<br />
: 04 to 05<br />
| [http://www.asmrm2013.com/ ASMRM 2013] <br />
| 10<sup>th</sup> Conference of the Asian Society of Mitochondrial Research and Medicine ([[ASMRM]]) <br />
| Seoul, Korea<br />
|-<br />
|<br />
: 04<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-workshops O2k-Workshop IOC81]<br />
| ASMRM conference O2k-Workshop on [[High-Resolution Respirometry]]<br />
| Seoul, Korea<br />
|-<br />
|<br />
: 06 to 07<br />
| [http://mitochondria2013.org J-mit]<br />
| International Symposium on Mitochondria 2013 and the 13<sup>th</sup> Conference of Japanese Society of Mitochondrial Research and Medicine<br />
| Tokyo, Japan <br />
|-<br />
|<br />
: 07 to 09<br />
| [http://cepa2013.univ-lyon.fr CEPA 2013] <br />
| Colloque d'EcoPhysiology Animale] <br />
| Lyon, France<br />
|-<br />
|<br />
: 11 to 15<br />
| [http://researchnetworks.ki.se/converis/area/241 Course Karolinska Institute] <br />
| Course on Cancer ell energy metabolism<br />
| Stockolm, Norway<br />
|-<br />
|<br />
: 20 to 24<br />
| [http://www.sfrbm.org/sections/annual-meeting/20th-annual-meeting 20th SFRBM] <br />
| 20th Meeting of the Society for Free Radical Biology and Medicine (SFRBM) <br />
| San Antonio, TX, US<br />
|-<br />
| '''October'''<br />
|-<br />
|<br />
: 02 to 04<br />
| [https://registration.hinxton.wellcome.ac.uk/display_info.asp?id=349 WT Scientific conferences]<br />
| Mitochondrial Disease: Translating biology into new treatments<br />
| Cambridge, United Kingdom<br />
|-<br />
|<br />
: 09 to 14<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?IOC80_schroecken O2k-Fluorometry Workshop IOC80]<br />
| OROBOROS O2k-Fluorometry Workshop on [[High-Resolution Respirometry]] (HRR)<br />
| Schroecken, Austria<br />
|-<br />
|<br />
: 10 to 12<br />
| [http://events.embo.org/13-mac/ MAC'13]<br />
| Mitochondria, Apoptosis and Cancer 2013<br />
| Stockholm, Sweden<br />
|-<br />
|<br />
: 14 to 17<br />
| [http://www.oxyclubcalifornia.org/SFRBM_SA/index.php SFRBM-SA]<br />
| VIII Society Free Radical Biology and Medicine-South America <br />
| Buenos Aires, Argentinia<br />
|-<br />
|<br />
: 16 to 19<br />
| [http://oxyclubcalifornia.org/ICPH6/index.php ICPH6]<br />
| 6<sup>th</sup> International Conference on Polyphenols and Health<br />
| Buenos Aires, Argentina<br />
|-<br />
|<br />
: 17 to 18<br />
| [http://www.targeting-mitochondria.com/?utm_source=Sarbacane&utm_medium=email&utm_campaign=09%2F01%2F2013+Mitochondrie+-+At+your+diary+-+9+janvier Targeting Mitochondria]<br />
| 4<sup>th</sup> World Congress on Targeting Mitochondria <br />
| Berlin, Germany<br />
|-<br />
|<br />
: 24 to 25<br />
| [http://www.mobilehealth-site.com/newsletter.php?3&10272-267&15&o4A2vHpKvW8LBw%2F7AIoeAQ Mobilehealth]<br />
| Diabetes & Obesity World Congress<br />
| Riga, Latvia<br />
|-<br />
|<br />
: 25 to 26<br />
| [http://www.mito-medizin.de/fortbildung/ Curriculum 2013]<br />
| Modular course by IGRMM ''Fortbildung Klinische Mitochondrien-Medizin und Umweltmedizin - Kurs E''<br />
| Speyer, Germany<br />
|-<br />
|<br />
: 28 to Nov 01<br />
| [http://www.fbs.osaka-u.ac.jp/DynaMito2013/DynaMito2013/Welcome.html DynaMito2013]<br />
| 4 <sup>th</sup> International Symposium on Dynamics of Mitochondria (DynaMito 2013)<br />
| Okinawa, Japan<br />
|-<br />
| '''September'''<br />
|-<br />
|<br />
: 12 to 15<br />
| [http://oxidation2013.org.au/ Peroxidase Meeting 2013]<br />
| 8th International Peroxidase Meeting<br />
| Sydney, Australia<br />
|-<br />
|<br />
: 18 to 20<br />
| [http://www.uib.no/rg/mitochondrial_medicine/news/scandomit-2010 Scandomit]<br />
| 2013 ScandoMit conference<br />
| Oulu, Finland<br />
|-<br />
|<br />
: 19 to 23<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?IOC79_obergurgl O2k-Workshop IOC79]<br />
| OROBOROS O2k-Fluorometry-Workshop on [[High-Resolution Respirometry]] (HRR), MiP2013 pre-conference workshop. University of Innsbruck - Conference Centre Obergurgl.<br />
| Obergurgl, Austria<br />
|-<br />
|<br />
: 23 to 27<br />
| [[Image:MiPsocietyLOGO.JPG|left|90px|link=http://www.mitophysiology.org|MiPsociety]][http://www.mitophysiology.org/?MiP2013 MiP2013]<br />
| 9<sup>th</sup> MiP''conference'' - Comparative Mitochondrial Physiology: 10 years [[MiP]]''society'' - in honour of Kjell Johansen and Peter Hochachka. University of Innsbruck - Conference Centre Obergurgl.<br />
| Obergurgl, Austria<br />
|-<br />
|<br />
: 23 to 27<br />
| [http://www.easd2013.com/ EASD]<br />
| 49<sup>th</sup> EASD Annual Meeting<br />
|-<br />
|<br />
: 25 to 29<br />
| [http://gfbioenergetique.free.fr/Carry2013/indexCarry.html GFB]<br />
| 17<sup>th</sup> Congress of Bioenergetics French Group <br />
| Carry-le-Rouet, Marseille, France<br />
|-<br />
|<br />
: 27 to Sept 28<br />
| [http://www.mito-medizin.de/fortbildung/ Curriculum 2013]<br />
| Modular course by IGRMM ''Fortbildung Klinische Mitochondrien-Medizin und Umweltmedizin - Kurs D''<br />
| Speyer, Germany<br />
|-<br />
| '''August'''<br />
|-<br />
|<br />
: 26 to 30<br />
| [[Image:MiPsocietyLOGO.JPG|left|90px|link=http://www.mitophysiology.org|MiPsociety]][http://www.conferencemanager.dk/MiP2013/home.html MiP''summer'' 2013]<br />
| 6<sup>th</sup> MiP''summer'' School on Mitochondrial Physiology - University of Copenhagen<br />
| Copenhagen, Denmark<br />
|-<br />
| '''July'''<br />
|-<br />
|<br />
: 03 to 04<br />
| [http://www.mobilehealth-site.com/newsletter.php?3&10272-266&9&iiMAyAFbJdkGtRFWpz%2FyeA Oxidative Stress & Antioxidants 2013]<br />
| 12th International Conference on Oxidative Stress & Antioxidants<br />
| Paris, France<br />
|-<br />
| '''June'''<br />
|-<br />
|<br />
: 05 to 07<br />
| [http://oxyclubcalifornia.org/Paris2013/index.php SFRR Symposium]<br />
| French Society of Free Radical Research: 5<sup>th</sup> International Symposium: nutrition, oxygen biology and medicine<br />
| Paris, France<br />
|-<br />
|<br />
: 07 to 08<br />
| [http://www.ic2013.medicres.org/ medicReS Conference on Good Clinical Research]<br />
| International CME Conference on Good Clinical Research<br />
| Istanbul, Turkey<br />
|-<br />
|<br />
: 08 to 12<br />
| [http://www.hypoxia-finland.com/general-information/ Dealing with Hypoxia]<br />
| Oxygen 2013 conference of the [http://www.hypoxianet.com/about/organizers/ ''HypoxiaNet''] <br />
| Oulu, Finland<br />
|-<br />
|<br />
: 12 to 15<br />
| [http://www.umdf.org/site/c.piKYL1PHLtF/b.4864979/k.C003/Home.htm UMDF]<br />
| [[UMDF]] Conference - Mitochondrial Medicine<br />
| Newport Beach, CA, USA<br />
|-<br />
|<br />
: 13<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-workshops O2k-Workshop IOC77]<br />
| UMDF conference O2k-Workshop on [[High-Resolution Respirometry]]<br />
| Newport Beach, CA, USA<br />
|-<br />
|<br />
: 13 to 14<br />
| [http://www.mcc-studio.org/index.php?option=com_flexicontent&view=items&cid=46:2013&id=264:8th-forum-on-oxidative-stress-and-aging&Itemid=185 Forum on oxidative Stress]<br />
| International Forum on Oxidative Stress and Aging<br />
| Bologna, IT<br />
|-<br />
|<br />
: 16 to 21<br />
| [https://secure.faseb.org/FASEB/meetings/summrconf/Programs/11612.pdf FASEB ''Mitochondria'']<br />
| FASEB: Mitochondrial Biogenesis and Dynamics in Health Disease and Aging<br />
| Big Sky, Montana, USA<br />
|-<br />
|<br />
: 18 to 19<br />
| [[International Society on Oxygen Transport to Tissue|ISOTT]]<br />
| International Symposium on Metabolic Imaging and Spectroscopy Honoring the 100<sup>th</sup> Birthday of Britton Chance. University of Pennsylvania.<br />
| Philadelphia, USA<br />
|-<br />
|<br />
: 23 to 28<br />
| [http://www.grc.org/programs.aspx?year=2013&program=bioenerg GRC]<br />
| Gordon Research Conference on Bioenergetics ''Molecular Mechanisms and Fundamental Principles to Cellular Energetics in Health and Disease''<br />
| Proctor Academy, Andover, New Hampshire, USA<br />
|-<br />
|<br />
: 26 to 28<br />
| [http://www.isanh-me.com/?utm_source=Sarbacane&utm_medium=email&utm_campaign=29%2F04%2F2013+POlyphenols+-+29+avril International Conference on Antioxidants]<br />
| International Conference on Antioxidants<br />
| Marrakech, Morocco<br />
|-<br />
| '''May'''<br />
|-<br />
|<br />
: 04<br />
| [http://mitoaction.org/clinicalconference Mitoaction Clinical Conference]<br />
| 2013 Mitochondrial Disease Clinical Conference<br />
| Cambridge, MA, US<br />
|-<br />
|<br />
: 05 to 07<br />
| [http://www.cell-symposia-mitochondria.com/index.html Cell Symposia]<br />
| Symposium on Mitochondria: from Signaling to Disease<br />
| Lisbon, Portugal<br />
|-<br />
|<br />
: 06 to 07<br />
| [http://www.cvent.com/events/nhlbi-mitochondrial-biology-symposium-2013/event-summary-26f05e0e129748c8b816fafd1479f2de.aspx NHLBI2013]<br />
| 2013 NHLBI Mitochondrial Biology Symposium: Mitochondrial Genetics in Health and Disease<br />
| Bethesda, Maryland, USA<br />
|-<br />
|<br />
: 15 to 18<br />
| [http://lpi.oregonstate.edu/conf2013/index.html Diet and Optimum Health Conference]<br />
| Linus Pauling Institute: 7<sup>th</sup> conference on ''Diet and Optimum Health''<br />
| Oregon State University, Corvallis, Oregon, USA<br />
|-<br />
|<br />
: 26 to 31<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?IOC76_prague O2k-Workshop IOC76]<br />
| OROBOROS O2k-Workshop on [[High-Resolution Respirometry]] and O2k-Fluorometry<br />
| Prague, Czech Republic <br />
|-<br />
| '''April'''<br />
|-<br />
|<br />
: 10 to 13<br />
| [http://www.biochemistry.org/Conferences/AllConferences/tabid/379/Page/1/MeetingNo/SA150/view/Conference/Default.aspx 3<sup>rd</sup> Joint German/UK Bioenergetics Conference ]<br />
| 3<sup>rd</sup> D/UK Bioenergetics Conference on Bioenergetics (DUKBEC) 2013: Bioenergetics in mitochondria, bacteria and chloroplasts. Biochemical Society and Max Planck Institute of Biophysics.<br />
| Ebsdorfergrund, Germany<br />
|-<br />
|<br />
: 09 to 14<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?IOC75_schroecken O2k-Workshop IOC75]<br />
| OROBOROS O2k-Workshop on [[High-Resolution Respirometry]] (HRR)<br />
| Schroecken, Austria<br />
|-<br />
|<br />
: 14 to 19<br />
| [http://www.grc.org/programs.aspx?year=2013&program=oxidat GRC]<br />
| The 2013 Gordon Research Conference on OXIDATIVE STRESS AND DISEASE: The Metabolic – Inflammatory Axis in Brain Aging and Alzheimer’s Disease<br />
| Les Diablerets, Switzerland <br />
|-<br />
| <br />
: 17 to 20<br />
| [http://www.esci.eu.com/meetings/ ESCI2013] [http://www.esci.eu.com/wp-content/uploads/2012/11/ESCI-2013_Programme-ws-2_20-November-2012.doc Workshop-Programme]<br />
| 47<sup>th</sup> Annual Scientific Meeting of the European Society for Clinical Investigation incl. a two-day mitochondrial workshop entitled ''Mitochondrial physiology: from basic research to the clinic''<br />
| Albufeira, Portugal<br />
|-<br />
|<br />
: 24<br />
| [http://www.abcam.com/index.html?pageconfig=resource&rid=15718 UCL CfMR/Abcam]<br />
| UCL CfMR/Abcam mitochondrial symposium: ''Mitochondria, the cardiovascular system and metabolic syndrome''<br />
| Kennedy Lecture Theatre, Institute of Child Health, Guildford Street, London, UK <br />
|-<br />
|<br />
: 26 to 27<br />
| [http://www.mito-medizin.de IGRMM]<br />
| Curriculum MitoMedizin, Kurs B<br />
| Heidelberg, Germany<br />
|-<br />
<br />
|-<br />
| '''March'''<br />
|-<br />
|<br />
: 08 to Oct 26<br />
| [http://www.mito-medizin.de/fortbildung/ Curriculum 2013]<br />
| Modular course by IGRMM ''Fortbildung Klinische Mitochondrien-Medizin und Umweltmedizin''<br />
| Speyer, Germany<br />
|-<br />
|<br />
: 15 to 17<br />
| [http://www.bio.unipd.it/bam/ BAM]<br />
| Padova Muscle Days<br />
| Padova, Italy<br />
|-<br />
|-<br />
| '''February'''<br />
|-<br />
|<br />
: 13 to 16<br />
| [http://www.zingconferences.com/index.cfm?page=conference&intConferenceID=103 Zing Aging Conference]<br />
| Mitochondria, metabolic regulation and the Biology of Aging Conference 2013<br />
| Lanzarote, Spain<br />
|-<br />
|<br />
: 18 to 21<br />
| [[UCL Consortium for Mitochondrial Research]]<br />
| Joint Anglo-Israeli Mitochondrial Biology Workshop, Weizmann Institute<br />
| Rehovot, Israel<br />
|-<br />
|<br />
: 24 to Mar 03<br />
| [http://www.neopterin.net/ Neopterin]<br />
| 32<sup>nd</sup> International Winter-Workshop on Clinical, Chemical and Biochemical Aspects of Pteridines<br />
| St. Christoph, Arlberg, Austria<br />
|-<br />
|<br />
: 26 to Mar 02<br />
| [http://www.hypoxia.net/index.htm Hypoxia]<br />
| Hypoxia XVIII. International Hypoxia Symposia<br />
| Lake Louise, Alberta, Canada<br />
|-<br />
| '''January'''<br />
|-<br />
|<br />
: 10 to 12<br />
| [http://eci2013.manipal.edu/ ECI2013]<br />
| Epigenomics and Cancer Informatics - Symposium and Workshop<br />
| Manipal, India<br />
|-<br />
|<br />
: 13 to 17<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?IOC73_kalkota O2k-Workshop IOC73] <br />
| Neurocon Pre-Conference Workshop on Recent Techniques in Neuroscience Research, HRR and OROBOROS Oxygraph-2k<br />
| Kolkata, India<br />
|-<br />
|<br />
: 18 to 20<br />
| [https://sites.google.com/site/neuroconkolkata/ Neurocon 2013]<br />
| International Conference on Neurodegenerative and Neurodevelopmental Disorders: Translational Aspects<br />
| Kolkata, India<br />
|-<br />
|<br />
: 21 to 27<br />
| [http://www.eblab.cl/wp-content/uploads/2011/09/Poster-v3.pdf Unveiling Mitochondria 2.0]<br />
| Unveiling Mitochondria 2.0: Hands on Training in Live Cell Imaging and Respirometry<br />
| Santiago, Chile<br />
|-<br />
|<br />
: 22 to 25<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?IOC74_innsbruck IOC74]<br />
| International O2k-Workshop on [[High-Resolution Respirometry]] (HRR) - TPP/ISE<br />
| Innsbruck, Austria<br />
|-<br />
|<br />
: 25 to 26<br />
| [http://www.klinikum.uni-heidelberg.de/High-Altitude-Tolerance.128939.0.html HAT2013]<br />
| Symposium ''High Altitude Tolerance''<br />
| Heidelberg, Germany<br />
|}<br />
<br />
<br />
<br />
<br />
<br />
[[Category:Mitochondrial Global Network]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet12.17_BovineHeartMito&diff=55334MiPNet12.17 BovineHeartMito2013-12-02T12:37:02Z<p>Bidaurratzaga Eider: /* Further information */</p>
<hr />
<div>{{Publication<br />
|title=Petrosyan S, Naviaux R, Haas RH, Gnaiger E (2007) Mitochondrial respiration with frozen bovine heart mitochondria: diagnostic tests. Mitochondr Physiol Network 12.17.<br />
|info=[[Media:MiPNet12.17 FrozenBovineHeartMito.pdf|'''Open Access-MiPNet12.17.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet12.17_FrozenBovineHeartMito.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=2007<br />
|journal=Mitochondr Physiol Network<br />
|abstract='''O2k-Protocol for Mitochondrial Respiration with Frozen Bovine Heart Mitochondria: Diagnostic Tests'''<br />
<br />
The following demo experiment was performed in preparation for the lecture hall experiment, presented at the Mitochondrial Medicine conference (Diagnosis of Disease: Workshop on HRR at Mitochondrial Medicine 2007, UMDF, San Diego, USA, June 2007 (IOC40). For explanation of symbols, see [http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways].<br />
<br />
[[Image:MiPNet12.17.jpg|center|100px|frame|Figure 1. Simultaneous display of oxygen concentration (blue lines) and oxygen flux (respiratory rate, red lines; negative time derivative of oxygen concentration) in chamber A and B.]]<br />
<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
}}<br />
{{Labeling<br />
|area=Respiration, Instruments;methods<br />
|organism=Bovines<br />
|tissues=Heart<br />
|preparations=SMP<br />
|topics=Coupling efficiency;uncoupling, Cyt c<br />
|couplingstates=OXPHOS<br />
|substratestates=CII<br />
|instruments=Oxygraph-2k, Protocol<br />
|additional=O2k-Demo<br />
}}<br />
== Further information ==<br />
:'''>> [http://www.oroboros.at/index.php?id=protocols_mitos_heart Excel Templates and DatLab-Demo Files]'''<br />
<br />
*Version 1: 2007<br />
*Version 11: 2011-12-11<br />
<br />
<br />
<br />
[[Category:OroboPedia]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet18.06_Amplex-Yeast&diff=55315MiPNet18.06 Amplex-Yeast2013-12-02T09:24:23Z<p>Bidaurratzaga Eider: /* Further information */</p>
<hr />
<div>{{Publication<br />
|title=Fontana-Ayoub M, Fasching M, Eigentler A, Laner V, Gnaiger E (2013) O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. Mitochondr Physiol Network 18.06.<br />
|info=[[Media:MiPNet18.06 Amplex-Yeast.pdf|'''Open Access-MiPNet18.06.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet18.06_Amplex-Yeast.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=2013<br />
|journal=Mitochondr Physiol Network<br />
|abstract='''O2k-Protocol for Amplex® UltraRed using freeze-dried baker’s yeast'''<br />
<br />
Respiration and hydrogen peroxide production of yeast was studied under hypoxic and hyperoxic conditions, and after stimulation of respiration by glucose using the [[O2k-Fluorescence LED2-Module]]. Extracellular H<sub>2</sub>O<sub>2</sub>-production by intact yeast cells was a pronounced function of environmental oxygen concentration (Fig. 1). Oxygen kinetics of respiration resembled closely but not fully that of isolated mitochondria, indicating that extracellular oxygen levels provide a good approximation of intracellular conditions, and intracellular oxygen gradients are small. Stimulation of respiration was pronounced with the extracellular substrates glucose and ethanol, and with uncoupler titrations. In contrast, these additions exerted a minor effect on extracellular H<sub>2</sub>O<sub>2</sub> production (Fig. 2).<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
|mipnetlab=AT Innsbruck OROBOROS, AT Innsbruck MitoCom<br />
|titel=O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast.<br />
|link=<br />
|editor=<br />
}}<br />
{{Labeling<br />
|area=Respiration<br />
|organism=Saccharomyces cerevisiae<br />
|taxonomic group=Fungi<br />
|preparations=Intact cells<br />
|topics=O2, Uncoupler<br />
|couplingstates=ROUTINE, ETS<br />
|instruments=Oxygraph-2k, Fluorometry, Protocol<br />
|additional=O2k-MultiSensor<br />
|titel=O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast.<br />
|link=<br />
|editor=<br />
}}<br />
== Further information ==</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet17.17_Amplex-Mouse-brain&diff=55314MiPNet17.17 Amplex-Mouse-brain2013-12-02T09:23:18Z<p>Bidaurratzaga Eider: /* Further Information */</p>
<hr />
<div>{{Publication<br />
|title=Fasching M, Sumbalova Z, Gnaiger E (2013) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</sub> production in mouse brain mitochondria. Mitochondr Physiol Network 17.17.<br />
|info=[[Media:MiPNet17.17_Amplex-Mouse-brain.pdf|'''Open Access-MiPNet17.17.pdf''']] ;[http://www.bioblast.at/index.php/File:MiPNet17.17_Amplex-Mouse-brain.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=2013<br />
|journal=Mitochondr Physiol Network<br />
|abstract='''O2k-Protocol for HRR and H<sub>2</sub>O<sub>2</sub> production in mouse brain mitochondria'''<br />
<br />
High-resolution respirometry [1] is extended by MultiSensor techniques allowing the simultaneous measurement of oxygen consumption and one additional parameter (mitochondrial membrane potential, pH, Ca<sup>2+</sup>, or NO concentration [2-5]). Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: H<sub>2</sub>O<sub>2</sub> production, mitochondrial membrane potential, intracellular pH, Ca<sup>2+</sup>, Mg<sup>2+</sup>, and NADH levels.<br />
Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber:<br />
# Quality control of preparations by respirometric performance.<br />
# Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP<sup>+</sup> or fluorophores.<br />
# Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber.<br />
# Additional information is acquired for a limited amount of sample.<br />
# Oxygen dependence of parameters can be studied.<br />
Here we introduce the O2k-Fluorescence LED2-Module, describe one selected stage in its development, and show its application for the simultaneous measurement of respiration and H<sub>2</sub>O<sub>2</sub> production of mouse brain mitochondria.<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
|mipnetlab=AT_Innsbruck_OROBOROS, AT Innsbruck Gnaiger E, AT Innsbruck MitoCom<br />
|articletype=MiPNet-online Publication<br />
}}<br />
{{Labeling<br />
|area=Respiration, Instruments;methods<br />
|organism=Mouse<br />
|tissues=Nervous system<br />
|preparations=Isolated Mitochondria<br />
|topics=Uncoupler<br />
|couplingstates=LEAK, OXPHOS, ETS<br />
|substratestates=CI, CII, CIII, ROX<br />
|instruments=Oxygraph-2k, Fluorometry, Protocol<br />
|articletype=MiPNet-online Publication<br />
}}<br />
== Further Information ==<br />
<br />
'''References'''<br />
<br />
# [[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]]<br />
# [[Aguirre 2010 Biochim Biophys Acta|<br />
Aguirre E, Rodríguez-Juárez F, Bellelli A, Gnaiger E, Cadenas S (2010) Kinetic model of the inhibition of respiration by endogenous nitric oxide in intact cells. Biochim Biophys Acta 1797: 557-565.]]<br />
# [http://www.oroboros.at/index.php?mipnetanalyzer MiPNetAnalyzer]<br />
# [[Hickey_2012_J_Comp_Physiol_B|Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J Comp Physiol B 182: 91-100.]]<br />
# Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2013) Combined high-resolution respirometry and fluorometry. Validation of safranin for determination of mitochondrial membrane potential. Methods Enzymol (in press).<br />
# [[Gnaiger 2012 MitoPathways|Gnaiger E (2012) Mitochondrial Pathways and Respiratory Control. An Introduction to OXPHOS Analysis. 3rd ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp.]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet17.04_CitrateSynthase&diff=55313MiPNet17.04 CitrateSynthase2013-12-02T09:22:29Z<p>Bidaurratzaga Eider: /* Further information */</p>
<hr />
<div>{{Publication<br />
|title=Eigentler A, Draxl A, Wiethüchter A, Kuznetsov AV, Lassnig B, Gnaiger E. Laboratory protocol: Citrate synthase. Mitochondrial marker enzyme. Mitochondr Physiol Network 17.04.<br />
|info=[[Media:MiPNet17.04 CitrateSynthase.pdf|'''Open Access-MiPNet17.04.pdf''']] ; [http://www.bioblast.at/index.php/File:MiPNet17.04_CitrateSynthase.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=*<br />
|journal=Mitochondr Physiol Network<br />
|abstract=<br />
'''O2k-Protocol for Citrate Synthase'''<br />
<br />
Citrate synthase (E.c. 4.1.3.7) is a pace-maker enzyme in the Krebs cycle (citric acid cycle). Citrate synthase, CS, has a molecular weight of 51,709 Da, with gene map locus 12q13.2-q13.3. CS is localized in the mitochondrial matrix, but is nuclear encoded, synthesized on cytoplasmic ribosomes and transported into the mitochondrial matrix. CS, therefore, is commonly used as a quantitative marker enzyme for the content of intact mitochondria (Holloszy et al 1970; Willimas et al 1986; Hood et al 1989), although this role of CS has been questioned in developmental (Drahota et al 2004), age-related studies (Marin-Garcia et al 1998), physical training ([[Pesta 2011 Am J Physiol Regul Integr Comp Physiol|Pesta et al 2011]]) and cardiac disease studies ([[Lemieux 2011 Int J Biochem Cell Biol|Lemieux et al 2011]]). Proliferation of mitochondria in pathological states is sometimes associated with an increase in CS activity per cell, but CS activity in a specific tissue is frequently constant when expressed per mitochondrial protein or per mt-respiratory capacity ([[Renner 2003 Biochim Biophys Acta|Renner et al 2003]]). Mitochondrial, cellular or tissue respiration, therefore, may be expressed per CS activity for specific applications ([[Kuznetsov 2002 Analyt Biochem|Kuznetsov et al 2002]]; [[Renner 2003 Biochim Biophys Acta|Renner et al 2003]]; [[Huetter 2004 Biochem J|Hütter el al 2004]]).<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
{{Labeling<br />
|area=Instruments;methods, mt-Biogenesis;mt-density<br />
|preparations=Enzyme<br />
|enzymes=Marker Enzyme<br />
|instruments=Protocol<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
<br />
<br />
== Further information ==<br />
<br />
:>> Continue the discussion:[[Talk:MiPNet17.04 CitrateSynthase]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet14.14_PermeabilizedFiberPreparation&diff=55312MiPNet14.14 PermeabilizedFiberPreparation2013-12-02T09:21:24Z<p>Bidaurratzaga Eider: /* Further Information */</p>
<hr />
<div>{{Publication<br />
|title=Pesta D, Gnaiger E (2009) Preparation of permeabilized muscle fibres for diagnosis of mitochondrial respiratory function. Mitochondr Physiol Network 14.14.<br />
|info=[[Media:MiPNet14.14 PermeabilizedFibrePreparation.pdf|'''Open Access-MiPNet14.14.pdf''']] ; [http://www.bioblast.at/index.php/File:MiPNet14.14_PermeabilizedFibrePreparation.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=2009<br />
|journal=Mitochondr Physiol Network<br />
|abstract='''O2k-Protocol for preparation of permeabilized muscle fibres for diagnosis.'''<br />
<br />
Application of [[permeabilized muscle fibres]] and [[high-resolution respirometry]] offer a sensitive diagnostic test of mitochondrial dysfunction in small [[biopsy]] specimens of human muscle. By using these techniques in conjunction with multiple [[substrate-uncoupler-inhibitor titration]] (SUIT) protocols, respirometric studies of human and animal tissue biopsies improve our fundamental understanding of mitochondrial respiratory control and the pathophysiology of mitochondrial myopathies.<br />
<br />
[[Image:MiPNet14.14.jpg|right|200px|thumb]]<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
}}<br />
{{Labeling<br />
|area=Respiration, Instruments;methods<br />
|organism=Human<br />
|tissues=Heart, Skeletal muscle<br />
|preparations=Permeabilized tissue<br />
|instruments=Protocol<br />
|additional=mt-Preparation<br />
}}<br />
== Further Information ==<br />
'''References'''<br />
<br />
* [[Pesta_2012_Methods_Mol_Biol| Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]]<br />
<br />
* [[Lemieux_2011_Int_J_Biochem_Cell_Biol| Lemieux H, Semsroth S, Antretter H, Höfer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43: 1729–1738.]]<br />
<br />
* [[Votion_2012_PLoS_One| Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7(4): e34890.]]<br />
<br />
<br />
:>> [[Pesta_2012_Methods Mol Biol]]<br />
:>> [http://www.oroboros.at/index.php?id=protocols_muscle_horse Respiration of horse muscle fibres]<br />
<br />
* Version 3: 2011-12-02<br />
* Version 2: 2010-10-12<br />
* Version 1: 2009<br />
<br />
===Preparation of Permeabilized Muscle Fibres===<br />
<br />
====Requirements====<br />
<br />
Ketamine, xylazine; 2 pairs of sharp scissors (large and small); 2 pairs of forceps with sharp tips (one straight tip, one angular tip; non-magnetic), 2 pairs of forceps with very sharp angular tips (non-magnetic); ice; tissue culture plate with 12 wells (Falcon 35/3043); 5 ml beaker; magnetic stirrer plate; small (3 ml) test tubes, microbalance (5 digits; 0.01 mg).<br />
<br />
BIOPSThe relaxing and biopsy preservation solution BIOPS contains 10 mM Ca-EGTA buffer, 0.1 µM free calcium, 20 mM imidazole, 20 mM taurine, 50 mM K-MES, 0.5 mM DTT, 6.56 mM MgCl2, 5.77 mM ATP, 15 mM phosphocreatine, pH 7.1 [MiPNet03.02]. BIOPS can be stored and shipped frozen at -20 °C.<br />
<br />
AnimalsAfter full anaesthesia of the the mouse or rat through intraperitoneal injection (e.g. Fentanyl, 1 ml/kg of mouse), the condition of the animal is checked (heart beat is visible, the reflex test on the hindlimb is negative).<br />
<br />
==== Tissue preparation ====<br />
<br />
The muscle (heart or skeletal muscle) is excised. Skeletal muscle is cut lengthwise into small samples of 10-20 mg Ww, and put into a Falcon tube with 10 ml of ice cold BIOPS. In this preservation solution, the sample can be stored for several hours at 0 °C, depending on the source of muscle tissue (e.g. mouse skeletal muscle for at least six hours; human skeletal muscle for up to 24 h; Skladal et al 1994). This provides the possibility for shipping of biopsies on ice for functional analysis by high-resolution respirometry. <br />
<br />
Place the tissue sample in ice cold preservation solution (BIOPS) into a small petri dish on ice. Remove all connecting tissue using the pair of sharp or very sharp forceps.<br />
<br />
==== Tissue Separation ====<br />
<br />
Fibre bundles are separated mechanically with the two pairs of very sharp forceps, in a small petri dish on ice. A period of five minutes is required for preparation of a 2-mg sample of fibres. The degree of separation may be evaluated by observing a change from red to pale colouring of the separated fibre bundles. At least during an initial start-up period, use of a dissecting scope is recommended for observation of the mechanical separation, during which fibres are partially teased appart and streched out. The tissue should still remain connected in a mesh-like framework. <br />
<br />
This preparation leads to partial permeabilization of skeletal muscle, but full permeabilization of cell membranes in heart (Kuznetsov et al. 2004) or liver tissue (Kuznetsov et al. 2002). <br />
<br />
After tissue separation, the fibre bundels are placed sequentially into 2 ml ice cold BIOPS into individual wells of a Falcon 12-well tissue culture plate. <br />
<br />
==== Tissue permeabilization ====<br />
<br />
After completion of separation of fibre bundles for two or more O2k-chambers, the fibre bundles are transferred quickly into 2 ml of icecold BIOPS, containing 20 µl of saponin stock solution (5 mg/ml; final concentration 50 µg/ml). <br />
<br />
Shake by gentle agitation in the cold room (on ice) for 30 min. After the 30 min period, all samples are quickly transferred from the saponin solution into 2 ml of MiR06 (see figure), and shaken by gentle agitation for 10 min in the cold room (on ice).<br />
<br />
Additional samples may prepared with or without saponin permeabilization for other assays (histochemical/morphological; enzymatic; mtDNA). <br />
<br />
=== Wet weight ===<br />
<br />
Weight measurements are made after permeabilization, eliminating variations in water contents due to osmotic stress, allowing for elimination of connective tissue during mechanical separation before weight measurement, and for partitioning of subsamples of similar wet weight.<br />
<br />
Before adding the tissue into the O2k chamber, take wet weight measurements of several loosly connected fibre bundles of c. 1-3 mg wet weight (skeletal muscle) or 0.5-2 mg wet weight (heart). Blot the bundles carefully on filter paper. Take the sample with the sharp pair of forceps (angular tip) and place it for 5 s onto the filter paper. During this time, wipe off any liquid from the tip of the forceps with another filter paper. Then take the sample from the filter paper and touch it once more shortly onto a dry area of filter paper while holding it with the forceps. Then immediately place the sample onto a small plastic plate on the table of the tared balance.<br />
<br />
Immediately after reading the wet weight, the sample is transferred into a well with 2 ml ice cold MiR06. Check that the tare balance reading returns to zero. Each well receives a sample for an O2k-chamber.<br />
<br />
=== Oxygraph-2k ===<br />
<br />
Calibrate the oxygen sensors in MiR06 at experimental temperature in the ‘open’ chamber (gas phase above the stirred medium, with a partially inserted stopper) of the Oxygraph-2k at a Gain of 1, to ensure that the raw signal does not reach the limiting value of 10 V at high experimental oxygen concentrations.<br />
<br />
Respiration is measured (at 37 °C for mammalian tissues) in mitochondrial respiration medium (MiR06) designed for optimal protection of mitochondrial function [MiPNet14.13]. From the vial with MiR06, the sample is transferred into the Oxygraph-2k chamber containing air saturated MiR06. The sample is immersed with the pair of straight forceps into the stirred medium in the O2k-chamber.<br />
<br />
After closing the chamber, the oxygen concentration is increased to 350 µM, adding microliter volumes of H2O2 solution, using the TIP2k or by manual titration [MiPNet14.06]. The oxygen signal increases rapidly at each titration step, and the titration is continued until the final oxygen level is reached. 5 to 10 min are required for stabilization of the signal. Oxygen concentration is prevented to drop below 200 µM by intermittent H2O2 titrations, preventing diffusion limitation of respiration in permeabilized fibres (Gnaiger 2003).<br />
<br />
Respiratory flux of muscle fibre bundles is limited by oxygen diffusion even above 50% air saturation (O2 concentration of 100 µM). This problem is carefully controlled and avoided by application of a high-oxygen regime. It is essential that instrumental and chemical oxygen background fluxes are routinely determined as a function of oxygen concentration in the experimental range. With appropriately calibrated background parameters inserted into the ‘Edit Experiment’ window of Datlab 4, background corrections are automatically obtained on-line over the entire experimental oxygen range. This is an indispensable prerequisite for accurate measurements particularly at low fluxes, when the amount of biological material is limited.<br />
<br />
=== References ===<br />
<br />
# [[Gnaiger_2003_Adv Exp Med Biol|Gnaiger E (2003) Oxygen conformance of cellular respiration. A perspective of mitochondrial physiology. Adv Exp Med Biol 543: 39-55.]]<br />
# [[Gnaiger_2000_MitoInTheCold|Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Heiderlberg, Berlin, New York: 431-442.]]<br />
# Lemieux H, Gnaiger E (2007-2008) Preparation of permeabilized muscle fibers for diagnosis of mitochondrial respiratory function. MiPNet12.22: 1-4.<br />
# [[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]]<br />
# Saks VA, Veksler VI, Kuznetsov AV, Kay L, Sikk P, Tiivel T, Tranqui L, Olivares J, Winkler K, Wiedemann F, Kunz WS (1998) Permeabilised cell and skinned fibre techniques in studies of mitochondrial function in vivo. Mol Cell Biochem 184: 81-100.<br />
# [[Skladal_1994_BTK|Skladal D, Sperl W, Schranzhofer R, Krismer M, Gnaiger E, Margreiter R, Gellerich FN (1994) Preservation of mitochondrial functions in human skeletal muscle during storage in high energy preservation solution (HEPS). In: What is Controlling Life? (Gnaiger E, Gellerich FN, Wyss M, eds) Modern Trends in BioThermoKinetics 3, Innsbruck Univ Press: 268-271.]]<br />
# Kuznetsov AV, Gnaiger E (1998) MiPNet03.01 and (1999) MiPNet04.07.<br />
# [[Kuznetsov_2004_Am J Physiol Heart Circ Physiol|Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome c release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286: H1633–H1641.]]<br />
<br />
Protocols:<br />
<br />
* [[MiPNet03.02]] Selected media and chemicals.<br />
* [[MiPNet14.06]] Instrumental background correction and accuracy of oxygen flux.<br />
* [[MiPNet14.13]] Mitochondrial respiration medium – MiR06.</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet14.13_Medium-MiR06&diff=55311MiPNet14.13 Medium-MiR062013-12-02T09:20:17Z<p>Bidaurratzaga Eider: /* Further information */</p>
<hr />
<div>{{Publication<br />
|title=Fasching M, Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) Mitochondrial respiration medium - MiR06. Mitochondr Physiol Network 14.13.<br />
|info=[[Media:MiPNet14.13 Medium-MiR06.pdf|'''Open Access-MiPNet14.13.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet14.13_Medium-MiR06.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=2013<br />
|journal=Mitochondr Physiol Network<br />
|abstract='''O2k-Protocol for Mitochondrial Respiration Medium - MiR06 '''<br />
<br />
Mitochondrial respiration medium MiR06 was developed for oxygraph incubations of mitochondrial preparations. MiR06 = MiR05 plus catalase. MiR06Cr = MiR06+creatine.<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
|keywords=MiR06<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
|discipline=Mitochondrial Physiology<br />
}}<br />
{{Labeling<br />
|area=Instruments;methods<br />
|instruments=Protocol<br />
|additional=Chemicals & Media<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
|discipline=Mitochondrial Physiology<br />
}}<br />
== Application of MiR06 in [[HRR]] ==<br />
<br />
'''MiR06: Mitochondrial Respiration Medium''' ([[#Preparation of MiR05 (MiR06) stock solution|MiR06]] = [[MiR05]] + [[Catalase]]).<br />
<br />
::Oxygen solubility factor in MiR05 or MiR06 at 30 °C and 37 °C = 0.92<br />
<br />
::pH of MiR05/06: 7.2 (20 °C), 7.2 (25 °C), 7.1 (30 °C), 7.1 (35 °C), 7.0 (37 °C)<br />
<br />
<br />
== Re-oxygenation with H<sub>2</sub>O<sub>2</sub> titrations ==<br />
<br />
* '''To increase oxygen levels''' small volumes (µl) of 200 mM H<sub>2</sub>O<sub>2</sub> stock solution are injected into the O2k-chamber filled with 2 ml MiR06.<br />
<br />
With MiR06 (or [[MiR06Cr]]), the medium in the O2k-chamber can be re-oxygenated very conveniently with H<sub>2</sub>O<sub>2</sub> titrations. The initial increase in oxygen, however, is preferentially made with oxygen gas, since there is the risk of bubble formation if the oxygen concentration is increased in a single large step. If oxygen gas is not available for the initial oxygenation, a very small bubble may be left in the chamber while slowly rising the oxygen level to 500 µM with additions of H<sub>2</sub>O<sub>2</sub>, such that gas can escape into the small bubble and then be extruded by fully closing the chamber. During the experiment, re-oxygenations are sufficiently small such that H<sub>2</sub>O<sub>2</sub> titrations into the closed chamber do not lead to gas bubble formation.<br />
<br />
== Preparation of MiR05 (MiR06) stock solution ==<br />
* Total volume of [[solution]] = 1 litre.<br />
::1) Weigh given amounts of the [[#Chemicals for MiR05|listed chemicals]] (except BSA and lactobionic acid) and transfer to a 1000 ml glass beaker.<br />
::2) Disrupt big lumps mechanically. It is recommended to do this before adding water, because during dissolution these lumps do not disintegrate easily.<br />
::3) Add ~800 ml H<sub>2</sub>O and dissolve using a magnetic stirrer at ~30 °C<br />
::4) Add 120 ml of [[#Preparation of K-lactobionate stock solution|K-lactobionate stock solution]].<br />
::5) Adjust the pH to 7.1 with 5 N KOH at 30 °C. <br />
::6) Dissolve the BSA in a subsample of the MiR05 stock solution and add to the final MiR05 (the separate preparation of the BSA solution is recommended, since BSA produces foams that do not dissolve easily).<br />
::7) Add H<sub>2</sub>0 to a final volume of 1000 ml.<br />
::8) Check pH again and adjust if necessary with small volumes of 5 N KOH. This solution is '''MiR05'''. MiR05 can be stored at -20 °C as described for MiR06.<br />
::9) To prepare <span style="color:#2E8B57"> '''MiR06'''</span>, add 280 000 units of catalase (100 mg of catalase powder containing 2800 u/mg solid) per litre MiR05 (280 units / ml final concentration).<br />
::10) Divide into 40 ml portions in plastic vials and store at -20 °C. <br />
::11) These storage solutions of MiR06 can be used as stock [[solution]]s. A vial is warmed up above experimental temperature, avoiding foam formation during gentle shaking. Up to 16 O2k-chambers can be filled with a 40 ml portion. It is recommended to use the stock solution on a single day only, to avoid any microbial contamination of the respiration medium.<br />
<br />
::<span style="color:#2E8B57"> '''MiR06''' </span> can also be prepared by adding 5 µl of the [[#Preparation of catalase stock solution|catalase stock solution]] directly into the O2k-chamber filled with MiR05 at the start of the experiment. The final catalase concentration in the 2 ml Oxygraph-2k chamber is 280 u/ml.<br />
<br />
<br />
=== Chemicals for MiR05/MiR06 ===<br />
<br />
<span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span><br />
{|border="1" cellpadding="3" cellspacing="0"<br />
|+<br />
!Compound<br />
!Final conc.<br />
!FW<br />
!Addition to 1 litre final volume<br />
!Company, product code and storage<br />
|-<br />
|align="right"|EGTA <br />
|align="right"|0.5 mM<br />
|align="right"|380.4<br />
|align="right"|0.190 g<br />
|align="right"|Sigma E 4378, 25 g, store at R.T.<br />
|-<br />
|align="right"|MgCl<sub>2</sub>.6H<sub>2</sub>0<br />
|align="right"|3 mM<br />
|align="right"|203.3<br />
|align="right"|0.610 g<br />
|align="right"|Scharlau MA 0036, 250 g, store at R.T.<br />
|-<br />
|align="right"|Taurine<br />
|align="right"|20 mM<br />
|align="right"|125.1<br />
|align="right"|2.502 g<br />
|align="right"|Sigma T 0625, 25 g, store at R.T.<br />
|-<br />
|align="right"|KH<sub>2</sub>PO<sub>4</sub> <br />
|align="right"|10 mM<br />
|align="right"|136.1<br />
|align="right"|1.361 g<br />
|align="right"|Merck 104873 or Sigma P5655 1000 g, store at R.T.<br />
|-<br />
|align="right"|HEPES <br />
|align="right"|20 mM<br />
|align="right"|238.3<br />
|align="right"|4.77 g<br />
|align="right"|Sigma H 7523, 250 g, store at R.T.<br />
|-<br />
|align="right"|D-Sucrose<br />
|align="right"|110 mM<br />
|align="right"|342.3<br />
|align="right"|37.65 g<br />
|align="right"|Roth 4621.1, or Sigma 84097, 1000 g, store at R.T., ''Note a''<br />
|-<br />
|align="right"|BSA essentially fatty acid free <br />
|align="right"|1 g/l<br />
|align="right"|<br />
|align="right"|1 g<br />
|align="right"|Sigma A 6003 fraction V, 25 g, store at 2-8 °C<br />
|-<br />
|align="right"|Lactobionic acid<br />
|align="right"|60 mM<br />
|align="right"|358.3<br />
|align="right"|120 ml of 0.5 M [[#Preparation of K-lactobionate stock solution|K-lactobionate stock]]<br />
|align="right"|Aldrich 153516 or Sigma L2398, 100 g, store at R.T.<br />
|-<br />
|}<br />
<br />
* ''Note a:'' For specific applications contamination with glucose may be critical. Invert sugar (1:1 mixture of glucose and fructose) is specified for Roth #4621 (< 0.04%), but not for Sigma #84097. Invert sugar content is specified for Sigma #84100 (<0.05%), however the overall specified purity for this product (>= 99%) is lower than for Sigma #8407 and Roth #4621.1 (both products >= 99.5%).<br />
<br />
=== Preparation of K-lactobionate stock solution ===<br />
<br />
::1) weigh 35.83 g lactobionic acid into a 250 ml glass beaker<br />
::2) add 100 ml H<sub>2</sub>O and dissolve by stirring on magnetic stirrer<br />
::2) check pH (is approx. 2.0) and neutralize with 5 N KOH<br />
::4) adjust final volume to 200 ml with H<sub>2</sub>O. It is best to use a 200 ml volumetric glass flask.<br />
::5) check pH again and adjust to 7 if necessary (5 N KOH)<br />
<br />
<br />
=== Preparation of catalase stock solution ===<br />
<br />
::'''Catalase''' lypophilized powder, 2000-5000 '''units*'''/mg, Sigma C 9322, store at -20 °C<br />
<br />
::'''Stock solution:''' 112000 u/ml (dissolved in MiR05)<br />
<br />
<br />
::'''Example:''' 'Catalase lypophilized powder, 2800 units/mg solid and 3500 units/mg protein'<br />
<br />
::1) Use 'units/mg solid' for your calculations <br />
::2) Result: 40 mg catalase powder (2800 u/mg) are dissolved in 1 ml MiR05 to obtain a catalase stock solution with 112000 u/ml.<br />
::3) Titrate 5 µl of the catalase stock solution into the 2 ml chamber to achieve a final concentration of 280 IU/ml in the chamber.<br />
<br />
'''Unit definition:''' '''* Units''' of enzymatic activitiy (u) in µmol/min; assay used by Sigma Aldrich: ' ''One unit will decompose 1.0 μmol of H<sub>2</sub>O<sub>2</sub> per min at pH 7.0 at 25 °C, while the H<sub>2</sub>O<sub>2</sub> concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A<sub>240</sub>.'' '<br />
<br />
== MiR05Cr/MiR06Cr ==<br />
* [[MiR05Cr]] = MiR05 + Creatine<br />
* [[MiR06Cr]] = MiR06 + Creatine<br />
<br />
::1) Prepare fresh by adding 3 mg/ml creatine monohydrate (Fluka 27900) to MiR05 or MiR06.<br />
::2) Stirr gently on a magnetic stirrer.<br />
<br />
<br />
== Preparation of 200 mM H<sub>2</sub>O<sub>2</sub> stock solution ==<br />
<br />
'''H<sub>2</sub>O<sub>2</sub>''': Hydrogen peroxide solution, 50 wt. % in H2O, stabilized, Sigma 516813, store in the fridge. See [http://www.solvayasiapacific.com/static/wma/pdf/1/8/5/8/1/Hydrogen%20Peroxide%20Handling%20%20Storage%201%28English%20version%29.pdf this link] for handling and savety instructions concerning hydrogen peroxide.<br />
<br />
::1) Pipette 114 µl of 17.6 M H<sub>2</sub>O<sub>2</sub> into 10 ml plastic vial.<br />
::2) Add H<sub>2</sub>O to a total volume of 10 ml.<br />
::3) Wrap plastic vial in aluminium foil (solution is light sensitive) and store in the fridge.<br />
::4) During experiments keep this stock solution on ice.<br />
<br />
'''Titration''' of 3 µl of H<sub>2</sub>O<sub>2</sub> into the 2 ml O2k-chamber increases the concentration of O<sub>2</sub> by approx. 150 nmol/ml (150 µM).<br />
<br />
== Limitations of using MiR05 to MiR06Cr ==<br />
<br />
* MiR06 or MiR06Cr cannot be used for measurement of ROS production. Use MiR05 or MiR05Cr instead.<br />
* The high antioxidant activity may compete with reactions on which measurement of ROS production is based.<br />
* The intracellular milieu of kidney has a low [K<sup>+</sup>]. Kidney mitochondria are inhibited by the high [K<sup>+</sup>] of MiR05 to MiR06Cr [1].<br />
>> [[MiPMap#1._Human_and_model_organisms.2C_taxonomic_groups|MiPMap - Is this a general issue for the organ, or is it in addition also a species issue?]]<br />
<br />
# A mitochondrial respiration medium for kidney: [[Persson_2012_Diabetologia]].<br />
<br />
<br />
== Further information ==<br />
:>> [[MiPNet06.03_O2-Calibration-Solubility|Oxygen solubility in MiR06]]<br />
<br />
:>> [[List_of_media_for_respirometry|List of respiration media]]<br />
<br />
:>> MiPNet08.05 and MiPNet10.11 are integrated in MiPNet14.13_Medium-MiR06.<br />
<br />
* Version 3: 2013-06-20<br />
<br />
Original publication introducing MiR05:<br />
* [[Gnaiger 2000 Life in the Cold|Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York: pp 431-442.]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet14.06_Instrumental_O2_background&diff=55310MiPNet14.06 Instrumental O2 background2013-12-02T09:19:52Z<p>Bidaurratzaga Eider: /* Further information */</p>
<hr />
<div>{{Publication<br />
|title=Fasching M, Gnaiger E (2009) Instrumental background correction and accuracy of oxygen flux. Mitochondr Physiol Network 14.6.<br />
|info=[[Media:MiPNet14.06 InstrumentalBackground.pdf|'''Open Access-MiPNet14.06.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet14.06_InstrumentalBackground.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=2009<br />
|journal=Mitochondr Physiol Network<br />
|abstract=Correction for the instrumental background is a new and essential standard in high resolution respirometry, automatically performed by [http://www.oroboros.at/index.php?id=datlab DatLab]. Background measurements provide a test of instrument function. In the OROBOROS Oxygraph-2k, background corrections are usually within a few % of experimental flux over the entire experimental oxygen range. At minimum activities, however, even the small background effects become highly significant and require routine correction.<br />
<br />
[[Image:MiPNet14.06.jpg|centre|frame|Instrumental background experiment, measuring oxygen flux without biological sample at four oxygen levels (left), and linear relation between instrumental background oxygen flux and oxygen concentration (right). Modified after: Gnaiger E. (2001).]]<br />
<br />
Autoxidation of ascorbate and TMPD causes a [http://bioblast.at/images/0/0b/MiPNet06.06_ChemicalBackground.pdf chemical background oxygen flux]. DatLab provides on-line correction for instrumental and chemical background.<br />
<br />
[http://www.oroboros.at/index.php?id=backgroundcorrection#c3005 Excel Templates and DatLab-Demo Files] <br />
<br />
<br />
'''O2k-Protocol for Instrumental Background Correction and Accuracy of Oxygen Flux '''<br />
<br />
For calibration of the polarographic oxygen sensor (POS) and for measurement of instrumental background oxygen consumption, only incubation medium but no biological sample is added into the Oxygraph-2k chamber, at experimental conditions. In a closed chamber under these conditions, ideally oxygen concentration remains constant. In practice, however, instrumental background effects are caused by back-diffusion into the oxygraph chamber at low oxygen pressure, oxygen diffusion out of the oxygraph chamber at elevated oxygen levels, and oxygen consumption by the polarographic oxygen sensor (POS). Instrumental background interferes with accurate measurement of respiratory oxygen flux, if background effects remain undefined. Determination of instrumental background constitutes an important standard operating procedure (SOP) in high-resolution respirometry (HRR) Instrumental background oxygen flux is (i) minimized in the OROBOROS Oxygraph-2k by instrumental design and selection of appropriate materials. In addition, (ii) instrumenal background is routinely tested, and (iii) background correction of oxygen flux is applied automatically by DatLab.<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
|keywords=Instrumental background<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
{{Labeling<br />
|area=Respiration, Instruments;methods<br />
|instruments=Oxygraph-2k, Protocol<br />
|additional=O2k-SOP, DatLab<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
== Instrumental background test for permeabilized muscle fibres ==<br />
<br />
=== Extended instrumental background test ===<br />
<br />
# While biopsy sampling and fibre preparation proceed: Air calibration in [[MiR06Cr]], then close the chamber to evaluate instrumental background at air saturation (c. 10 min): This is a quality control of the medium, important under field conditions, where medium preservation (sterility) may be less controlled than in the lab. <br />
# Elevate oxygen concentration to 450 µM with oxygen gas ([[Syringe\60 ml\Gas-Injection]]), close and after two to three min perform a [[stirrer test]] (the new DatLab has an automatic stirrer test function, with defined duriation of stopping the stirrer and automatic restart of stirring, 40 s stirrer stop may be optimum). This is important, since the [[OroboPOS]] may have a different [[response time]] at elevated oxygen concentration. If the response time increases dramatically, then the sensor may even show a non-linear response to oxygen concentration at high oxygen levels. <br />
# Instrumental background: After 20 min, open the chamber and allow O<sub>2</sub> to drop to c. 350 µM, close for 20 min, open and drop O<sub>2</sub> to c. 250 µM (this should be the lowest experimental O<sub>2</sub> concentration). <br />
# Increase O<sub>2</sub> with H<sub>2</sub>O<sub>2</sub> injection (c. 2 µl) to 400 µM, measure for 15-20 min instrumental background, simulating a re-oxygenation during the experiment.<br />
# Increase O<sub>2</sub> with H<sub>2</sub>O<sub>2</sub> injection (c. 1 µl) to 450 µM, until the fibres are added, for equilibrating the instrument at high O<sub>2</sub>.<br />
# Addition of permeabilized fibres into the O2k-Chamber: >> [[Permeabilized muscle fibres]]<br />
<br />
<br />
== Further information ==<br />
'''References'''<br />
<br />
[[Gnaiger_1995_J_Bioenerg_Biomembr| Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27: 583-596.]]<br />
<br />
[[Gnaiger_2001_Respir_Physiol| Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128: 277-297.]]<br />
<br />
[[Gnaiger_2008_POS| Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352.]]<br />
<br />
<br />
:>> [http://www.oroboros.at/?BackgroundCorrection '''Excel Templates and DatLab-Demo Files''']<br />
:>> MiPNet10.07 is integrated in MiPNet14.06.<br />
<br />
* Version 2: 2011-12-04<br />
* Version 1: 2009<br />
<br />
<br />
<br />
[[Category:OroboPedia]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet14.06_Instrumental_O2_background&diff=55309MiPNet14.06 Instrumental O2 background2013-12-02T09:19:43Z<p>Bidaurratzaga Eider: /* Further information */</p>
<hr />
<div>{{Publication<br />
|title=Fasching M, Gnaiger E (2009) Instrumental background correction and accuracy of oxygen flux. Mitochondr Physiol Network 14.6.<br />
|info=[[Media:MiPNet14.06 InstrumentalBackground.pdf|'''Open Access-MiPNet14.06.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet14.06_InstrumentalBackground.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=2009<br />
|journal=Mitochondr Physiol Network<br />
|abstract=Correction for the instrumental background is a new and essential standard in high resolution respirometry, automatically performed by [http://www.oroboros.at/index.php?id=datlab DatLab]. Background measurements provide a test of instrument function. In the OROBOROS Oxygraph-2k, background corrections are usually within a few % of experimental flux over the entire experimental oxygen range. At minimum activities, however, even the small background effects become highly significant and require routine correction.<br />
<br />
[[Image:MiPNet14.06.jpg|centre|frame|Instrumental background experiment, measuring oxygen flux without biological sample at four oxygen levels (left), and linear relation between instrumental background oxygen flux and oxygen concentration (right). Modified after: Gnaiger E. (2001).]]<br />
<br />
Autoxidation of ascorbate and TMPD causes a [http://bioblast.at/images/0/0b/MiPNet06.06_ChemicalBackground.pdf chemical background oxygen flux]. DatLab provides on-line correction for instrumental and chemical background.<br />
<br />
[http://www.oroboros.at/index.php?id=backgroundcorrection#c3005 Excel Templates and DatLab-Demo Files] <br />
<br />
<br />
'''O2k-Protocol for Instrumental Background Correction and Accuracy of Oxygen Flux '''<br />
<br />
For calibration of the polarographic oxygen sensor (POS) and for measurement of instrumental background oxygen consumption, only incubation medium but no biological sample is added into the Oxygraph-2k chamber, at experimental conditions. In a closed chamber under these conditions, ideally oxygen concentration remains constant. In practice, however, instrumental background effects are caused by back-diffusion into the oxygraph chamber at low oxygen pressure, oxygen diffusion out of the oxygraph chamber at elevated oxygen levels, and oxygen consumption by the polarographic oxygen sensor (POS). Instrumental background interferes with accurate measurement of respiratory oxygen flux, if background effects remain undefined. Determination of instrumental background constitutes an important standard operating procedure (SOP) in high-resolution respirometry (HRR) Instrumental background oxygen flux is (i) minimized in the OROBOROS Oxygraph-2k by instrumental design and selection of appropriate materials. In addition, (ii) instrumenal background is routinely tested, and (iii) background correction of oxygen flux is applied automatically by DatLab.<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
|keywords=Instrumental background<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
{{Labeling<br />
|area=Respiration, Instruments;methods<br />
|instruments=Oxygraph-2k, Protocol<br />
|additional=O2k-SOP, DatLab<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
== Instrumental background test for permeabilized muscle fibres ==<br />
<br />
=== Extended instrumental background test ===<br />
<br />
# While biopsy sampling and fibre preparation proceed: Air calibration in [[MiR06Cr]], then close the chamber to evaluate instrumental background at air saturation (c. 10 min): This is a quality control of the medium, important under field conditions, where medium preservation (sterility) may be less controlled than in the lab. <br />
# Elevate oxygen concentration to 450 µM with oxygen gas ([[Syringe\60 ml\Gas-Injection]]), close and after two to three min perform a [[stirrer test]] (the new DatLab has an automatic stirrer test function, with defined duriation of stopping the stirrer and automatic restart of stirring, 40 s stirrer stop may be optimum). This is important, since the [[OroboPOS]] may have a different [[response time]] at elevated oxygen concentration. If the response time increases dramatically, then the sensor may even show a non-linear response to oxygen concentration at high oxygen levels. <br />
# Instrumental background: After 20 min, open the chamber and allow O<sub>2</sub> to drop to c. 350 µM, close for 20 min, open and drop O<sub>2</sub> to c. 250 µM (this should be the lowest experimental O<sub>2</sub> concentration). <br />
# Increase O<sub>2</sub> with H<sub>2</sub>O<sub>2</sub> injection (c. 2 µl) to 400 µM, measure for 15-20 min instrumental background, simulating a re-oxygenation during the experiment.<br />
# Increase O<sub>2</sub> with H<sub>2</sub>O<sub>2</sub> injection (c. 1 µl) to 450 µM, until the fibres are added, for equilibrating the instrument at high O<sub>2</sub>.<br />
# Addition of permeabilized fibres into the O2k-Chamber: >> [[Permeabilized muscle fibres]]<br />
<br />
<br />
== Further information ==<br />
'''References'''<br />
<br />
[[Gnaiger_1995_J_Bioenerg_Biomembr| Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27: 583-596.]]<br />
<br />
[[Gnaiger_2001_Respir_Physiol| Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128: 277-297.]]<br />
<br />
[[Gnaiger_2008_POS| Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352.]]<br />
<br />
<br />
:>> [http://www.oroboros.at/?BackgroundCorrection Intrumental '''Excel Templates and DatLab-Demo Files''']<br />
:>> MiPNet10.07 is integrated in MiPNet14.06.<br />
<br />
* Version 2: 2011-12-04<br />
* Version 1: 2009<br />
<br />
<br />
<br />
[[Category:OroboPedia]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet14.05_TPP-mtMembranePotential&diff=55308MiPNet14.05 TPP-mtMembranePotential2013-12-02T09:18:38Z<p>Bidaurratzaga Eider: /* Further information */</p>
<hr />
<div>{{Publication<br />
|title=Renner-Sattler K, Fasching M, Gnaiger E (2009) TPP+ and membrane potential. Mitochondr Physiol Network 14.05.<br />
|info=[[Media:MiPNet14.05 TPP-MitoMembranePotential.pdf|'''Open Access-MiPNet14.05.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet14.05_TPP-MitoMembranePotential.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=2009<br />
|journal=Mitochondr Physiol Network<br />
|abstract='''O2k-Protocol for Determination of Membrane Potential with TPP<sup>+</sup> and an Ion Selective Electrode System'''<br />
<br />
[[Image:MiPNet14.05.jpg|centre|frame|Sumbalova Z, Fasching M, Gnaiger E (2011) Substrate control in mitochondrial respiration and regulation of mitochondrial membrane potential. Abstract Mitochondrial Medicine Chicago.]]<br />
<br />
[http://www.bioblast.at/index.php/Sumbalova_2011Abstract_Mitochondrial_Medicine Abstract: Sumbalova Z, Fasching M, Gnaiger E (2011) Substrate control in mitochondrial respiration and regulation of mitochondrial membrane potential. Abstract Mitochondrial Medicine Chicago.]<br />
<br />
[http://bioblast.at/images/7/78/MiPNet14.05_TPP-MitoMembranePotential.pdf Appendix: Mathematics for Mitochondrial Membrane Potential]<br />
<br />
[http://www.oroboros.at/index.php?id=protocols_tpp-membranepotential#c2928 Excel Templates, DatLab Templates and DatLab-Demo Files]<br />
<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
|keywords=Archive<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
{{Labeling<br />
|instruments=Oxygraph-2k, TPP, Protocol<br />
|additional=O2k-Demo<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
== Further information ==<br />
:>> [http://www.oroboros.at/?protocols_tpp-membranepotential '''Excel Templates, DatLab Templates and DatLab-Demo Files''']<br />
<br />
* Version 3: 2011-12-11<br />
* Version 1: 2009</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet14.05_TPP-mtMembranePotential&diff=55307MiPNet14.05 TPP-mtMembranePotential2013-12-02T09:18:18Z<p>Bidaurratzaga Eider: /* Further information */</p>
<hr />
<div>{{Publication<br />
|title=Renner-Sattler K, Fasching M, Gnaiger E (2009) TPP+ and membrane potential. Mitochondr Physiol Network 14.05.<br />
|info=[[Media:MiPNet14.05 TPP-MitoMembranePotential.pdf|'''Open Access-MiPNet14.05.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet14.05_TPP-MitoMembranePotential.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=2009<br />
|journal=Mitochondr Physiol Network<br />
|abstract='''O2k-Protocol for Determination of Membrane Potential with TPP<sup>+</sup> and an Ion Selective Electrode System'''<br />
<br />
[[Image:MiPNet14.05.jpg|centre|frame|Sumbalova Z, Fasching M, Gnaiger E (2011) Substrate control in mitochondrial respiration and regulation of mitochondrial membrane potential. Abstract Mitochondrial Medicine Chicago.]]<br />
<br />
[http://www.bioblast.at/index.php/Sumbalova_2011Abstract_Mitochondrial_Medicine Abstract: Sumbalova Z, Fasching M, Gnaiger E (2011) Substrate control in mitochondrial respiration and regulation of mitochondrial membrane potential. Abstract Mitochondrial Medicine Chicago.]<br />
<br />
[http://bioblast.at/images/7/78/MiPNet14.05_TPP-MitoMembranePotential.pdf Appendix: Mathematics for Mitochondrial Membrane Potential]<br />
<br />
[http://www.oroboros.at/index.php?id=protocols_tpp-membranepotential#c2928 Excel Templates, DatLab Templates and DatLab-Demo Files]<br />
<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
|keywords=Archive<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
{{Labeling<br />
|instruments=Oxygraph-2k, TPP, Protocol<br />
|additional=O2k-Demo<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
== Further information ==<br />
''':>> [http://www.oroboros.at/?protocols_tpp-membranepotential Excel Templates, DatLab Templates and DatLab-Demo Files]'''<br />
<br />
* Version 3: 2011-12-11<br />
* Version 1: 2009</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet12.23_FiberRespiration&diff=55306MiPNet12.23 FiberRespiration2013-12-02T09:16:22Z<p>Bidaurratzaga Eider: /* Further information */</p>
<hr />
<div>{{Publication<br />
|title=Lemieux H, Votion DM, Gnaiger E (2007) Mitochondrial respiration in permeabilized fibres: Needle biopsies from horse skeletal muscle. Mitochondr Physiol Network 12.23.<br />
|info=[[Media:MiPNet12.23 FibreRespiration.pdf|'''Open Access-MiPNet12.23.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet12.23_FibreRespiration.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=2007<br />
|journal=Mitochondr Physiol Network<br />
|abstract='''O2k-Protocol for Mitochondrial Respiration in Permeabilized Fibres: Needle Biopsies from Horse Skeletal Muscle'''<br />
<br />
Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with permeabilized fibres in the OROBOROS Oxygraph-<br />
2k (O2k). The experiment demonstrates [http://bioblast.at/images/e/ef/MiPNet09.12_O2k-Titrations.pdf manual titrations] applied to study respiratory capacity in permeabilized fibres. Application of the [http://www.oroboros.at/?datlab DatLab 4 software] is shown for on-line data analysis [[MiPNet12.09]] The following guideline describes the experimental protocol and includes a short discussion of<br />
results. The experiments was carried out by participants of an O2k-Course on HRR in December 2007 [http://www.oroboros.at/index.php?id=ioc44 (IOC44; Schroecken, Austria).]<br />
<br />
[[Image:MiPNet12.23.jpg|center|100px|frame]]<br />
Figure 1. Oxygen concentration ([μM] blue line) and oxygen flux per mg wet weight of muscle ([pmol∙s-1∙mg-1] red lines) in O2k chamber B, in permeabilized fibres from horse skeletal muscle with the standard titration protocol.<br />
<br />
A multiple substrate-uncoupler-inhibitor titration protocol (Fig. 1) was developed for respiratory studies of permeabilized muscle fibres. The experiment was aimed at an assessment of a sequence of titrations, inducing stepwise defined respiratory states.<br />
<br />
<br />
<br />
#<span style="color:red"> '''LEAK state, L:'''</span> Non-phosphorylating resting state with substrates for Complex I (glutamate+malate; GMN; without adenalytes, N).<br />
#<span style="color:green">'''OXPHOS capacity with Complex I substrates, P:'''</span> Respiratory capacity in the active coupled state (with ADP, GMD).<br />
#<span style="color:black">'''Cytochrome c test for quality control:'''</span> Further addition of cytochrome c yields a test for integrity of the outer mitochondrial membrane (loss of cytochrome c would be indicated by a stimulation of respiration).<br />
#<span style="color:green">'''OXPHOS capacity with Complex I+II substrates, P:'''</span> Respiratory stimulation by convergent electron flow from Complexes I+II at the Q-junction, in the coupled state after further addition of succinate, as an estimate of OXPHOS capacity (Gnaiger 2009).<br />
#<span style="color:blue">'''Electron transfer system (ETS) capacity with CI+II substrates, E:'''</span> Uncoupling by FCCP titration (avoiding inhibition by high FCCP concentrations), as a test for limitation of OXPHOS relative to ETS capacity by the phosphorylation system.<br />
#<span style="color:blue">'''ETS capacity with CII substrate, E:'''</span> ETS capacity with succinate, after blocking Complex I with rotenone.<br />
#'''Residual oxygen consumption (ROX)''' due to oxidative side reactions in the permeabilized fibres, estimated after addition of Antimycin A (Inhibitor of Complex III).<br />
<br />
<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
|mipnetlab=AT_Innsbruck_OROBOROS, BE_Liege_Votion DM<br />
}}<br />
{{Labeling<br />
|area=Respiration, Instruments;methods<br />
|organism=Horse<br />
|tissues=Skeletal muscle<br />
|preparations=Permeabilized tissue<br />
|topics=Coupling efficiency;uncoupling, Cyt c<br />
|couplingstates=LEAK, OXPHOS, ETS<br />
|substratestates=CI, CII, CI+II<br />
|instruments=Oxygraph-2k, Protocol<br />
|additional=O2k-Demo<br />
}}<br />
== Further information ==<br />
:>> '''[http://www.oroboros.at/index.php?id=protocols_muscle_horse Excel Templates, DatLab Templates and DatLab-Demo Files]'''<br />
<br />
<br />
* Version 1: 2007<br />
* Version 6: 2011-12-11</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet11.05_imt-pti-pce&diff=55305MiPNet11.05 imt-pti-pce2013-12-02T09:01:39Z<p>Bidaurratzaga Eider: /* Further information */</p>
<hr />
<div>{{Publication<br />
|title=Gnaiger E (2005) Isolated mitochondria or permeabilized tissues and cells. Mitochondr Physiol Network 11.05.<br />
|info=[[Media:MiPNet11.05 Mitos-PermeabilizedCells.pdf|'''Open Access.MiPNet11.05.pdf''']], [http://www.bioblast.at/index.php/File:MiPNet11.05_Mitos-PermeabilizedCells.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=2005<br />
|journal=Mitochondr Physiol Network<br />
|abstract='''O2k-Protocol for Isolated mitochondria or permeabilized tissues and cells'''<br />
<br />
Summary: Whereas isolated mitochondria remain one of the gold-standards in studies of bioenergetics and mitochondrial physiology, permeabilized tissues and cells have become an alternative with several advantages. But some disadvantages have to be considered, too, for optimum experimental design and critical evaluation of results.<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
}}<br />
{{Labeling<br />
|area=mt-Biogenesis;mt-density<br />
|preparations=Permeabilized cells, Permeabilized tissue, Isolated Mitochondria<br />
|instruments=Protocol<br />
}}<br />
==Further information ==<br />
<br />
We recommend the new [http://at.mt.com/at/de/home/products/Laboratory_Weighing_Solutions/Analytical_Balances/Excellence_Analytical.html?com.broadvision.session.new=Yes METTLER TOLEDO] microbalances XS105DU or XS205DU for determination of tissue wet weight (1 to 3 mg wet weight per chamber). We offer these microbalances as part of our systems-approach to high-resolution respirometry.<br />
<br />
<br />
* Version 5: 2011-12-03<br />
* Version 1: 2005<br />
<br />
<br />
<br />
<br />
[[Category:OroboPedia]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet10.05_O2-Concentration-Flux&diff=55304MiPNet10.05 O2-Concentration-Flux2013-12-02T08:51:27Z<p>Bidaurratzaga Eider: /* Further Information */</p>
<hr />
<div>{{Publication<br />
|title=Gnaiger E (2005-2010) From oxygen concentration to oxygen flux. Mitochondr Physiol Network 10.05. - ''New edition:'' [[Gnaiger_2012_MitoPathways|Gnaiger 2012 Chapter 1]].<br />
|info=[[Media:MiPNet10.05 O2-Concentration-Flux.pdf|'''Open Access-MiPNet10.05.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet10.05_O2-Concentration-Flux.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|year=2005<br />
|journal=Mitochondr Physiol Network<br />
|abstract='''O2k-Protocol for Oxygen flux<br />
In a closed oxygraph chamber, the oxygen concentration declines over time as a result of respiratory processes. The time derivative, therefore, is a negative number. Why is then the ‘rate of oxygen consumption’ not expressed as a negative value? Why is the term ‘oxygen flux’ used in this context of chemical reactions? The rationale is based on fundamental concepts of physical chemistry and non-equilibrium thermodynamics.<br />
<br />
[[Image:MiPNet10.05.jpg|centre|500px|thumb]]<br />
<br />
Respiratory oxygen flux: On-line display of oxygen concentration (blue) and oxygen flux (respiration, red). Endogenous respiration of endothelial cells leads to oxygen depletion, followed by reoxygenations (dotted arrows). Cell membrane permeabilization by digitonin causes a decline of respiration to the resting level (without adenylates in the medium, -ANP). ADP titration activates respiration about 2-fold above the endogenous level of oxygen consumption.<br />
<br />
Eye-fitted slopes of oxygen chart recorder traces belong to the past. With [http://www.oroboros.at/index.php?id=datlab DatLab], trends are resolved. Accuracy is improved by standard numerical corrections. Graphs and protocols are stored and printed ready for publication.<br />
<br />
<br />
'''Reference'''<br />
<br />
[[Gnaiger_1993_Pure_Appl_Chem| Gnaiger E (1993) Nonequilibrium thermodynamics of energy transformations. Pure Appl Chem 65: 1983-2002.]]<br />
<br />
<br />
<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
|keywords=Archive<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
{{Labeling<br />
|instruments=Protocol<br />
|additional=O2k-SOP<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
== Further Information==<br />
:>> [http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet10.04_CellRespiration&diff=55301MiPNet10.04 CellRespiration2013-12-02T08:48:02Z<p>Bidaurratzaga Eider: /* Further information */</p>
<hr />
<div>{{Publication<br />
|title=Garedew A, Haffner B, Huetter E, Gnaiger E (2005) An experiment with high-resolution respirometry: Phosphorylation control in cell respiration. Mitochondr Physiol Network 10.04.<br />
|info=[[Media:MiPNet10.04 CellRespiration.pdf|'''Open Access-MiPNet10.04.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet10.04_CellRespiration.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=2005<br />
|journal=Mitochondr Physiol Network<br />
|abstract='''O2k-Protocol for Phosphorylation Control in Cell Respiration'''<br />
<br />
Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with cultured, suspended cells in the OROBOROS<br />
Oxygraph-2k (O2k). The experiment demonstrates manual [http://bioblast.at/images/e/ef/MiPNet09.12_O2k-Titrations.pdf titrations] of inhibitors, and automatic titrations of an uncoupler using the electronic Titration-Injection microPump [http://www.bioblast.at/images/8/8e/MiPNet04.05_Titration-Injection.pdf TIP2k ]. Application of the DatLab 4.3 (upgraded) software is shown for instrumental control (O2k and TIP2k), and [http://www.bioblast.at/images/2/28/MiPNet12.09_O2FluxAnalysis.pdf on-line data analysis ]. The following guideline describes the experiment in the form of a laboratory protocol, complementary to the relevant sections of the Oxygraph-2k Manual. The experiments were carried out by participants of an O2k-Course on high-resolution respirometry in April 2005 (IOC30; Schröcken, Austria).<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
<br />
[[Image:MiPNet10.04.jpg|center|width=80px|frame|Figure 1. Respiration of 32D cells (1.1106 ml-1). Traces for the two chambers of the Oxygraph-2k are superimposed. A: Oxygen flow [pmol O2s-1106]; B: Oxygen concentration [μM] (graph layout “7 Gr1-Flux Gr2-O2 Conc”). After inhibition of ATP synthase with oligomycin, FCCP was titrated at 0.5 μM steps with the Tip2k. Each titration step is automatically marked by an event (vertical lines). After the aerobic-anoxic transition, the chambers were opened for re-oxygenation, causing a 10-min disturbance of the traces of respiratory flow. The Tip2k needles were removed before opening, and re-inserted after closing the cham.]]<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
}}<br />
{{Labeling<br />
|area=Respiration, Instruments;methods<br />
|organism=Mouse<br />
|tissues=Blood cells<br />
|preparations=Intact cells<br />
|topics=Coupling efficiency;uncoupling, Uncoupler<br />
|couplingstates=LEAK, ROUTINE, ETS<br />
|substratestates=ROX<br />
|instruments=Oxygraph-2k, TIP2k, Protocol<br />
|additional=O2k-Demo<br />
}}<br />
== Further information ==<br />
:>> [http://www.oroboros.at/?protocols_cell_pcp Excel Templates and DatLab-Demo Files]<br />
* Version 6: 2012-04-07<br />
* Version 1: 2005<br />
<br />
* [[Gnaiger 2012 MitoPathways References|References]]<br />
<br />
<br />
<br />
[[Category:OroboPedia]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet09.12_O2k-Titrations&diff=55299MiPNet09.12 O2k-Titrations2013-12-02T08:46:17Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{Publication<br />
|title=OROBOROS (2013) Oxygraph-2k titrations: Mitochondria, permeabilized cells and biopsies. Mitochondr Physiol Network 09.12. Version 9.<br />
|info=[[Media:MiPNet09.12 O2k-Titrations.pdf|'''Open Access-MiPNet09.12.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet09.12_O2k-Titrations.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=2004<br />
|journal=Mitochondr Physiol Network<br />
|abstract='''O2k-Protocol for Mitochondria, Permeabilized Cells, and Biopsies'''<br />
<br />
A list of substrates, uncouplers, and inhibitors used in [[SUIT]] protocols in [[high-resolution respirometry]], with information on abbreviations, stock concentrations, final experimental concentrations, volumes titrated into the 2 ml O2k-chamber, and syringes used for manual titrations.<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
{{Labeling<br />
|area=Instruments;methods<br />
|topics=ATP; ADP; AMP; PCr, Substrate; Glucose; TCA Cycle, Amino Acid<br />
|instruments=Protocol<br />
|additional=Chemicals & Media<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
== Further information ==<br />
:>> [[http://www.oroboros.at/?protocols_titrations Excel Templates and DatLab-Demo Files]<br />
* Version 8: [[Gnaiger 2012 MitoPathways]]<br />
* Version 1: 2004<br />
<br />
<br />
[[Category:OroboPedia]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet09.12_O2k-Titrations&diff=55298MiPNet09.12 O2k-Titrations2013-12-02T08:44:01Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{Publication<br />
|title=OROBOROS (2013) Oxygraph-2k titrations: Mitochondria, permeabilized cells and biopsies. Mitochondr Physiol Network 09.12. Version 9.<br />
|info=[[Media:MiPNet09.12 O2k-Titrations.pdf|'''Open Access-MiPNet09.12.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet09.12_O2k-Titrations.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=2004<br />
|journal=Mitochondr Physiol Network<br />
|abstract='''O2k-Protocol for Mitochondria, Permeabilized Cells, and Biopsies'''<br />
<br />
A list of substrates, uncouplers, and inhibitors used in [[SUIT]] protocols in [[high-resolution respirometry]], with information on abbreviations, stock concentrations, final experimental concentrations, volumes titrated into the 2 ml O2k-chamber, and syringes used for manual titrations.<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
{{Labeling<br />
|area=Instruments;methods<br />
|topics=ATP; ADP; AMP; PCr, Substrate; Glucose; TCA Cycle, Amino Acid<br />
|instruments=Protocol<br />
|additional=Chemicals & Media<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
== Further information ==<br />
<br />
* Version 8: [[Gnaiger 2012 MitoPathways]]<br />
* Version 1: 2004<br />
<br />
<br />
[[Category:OroboPedia]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet08.18_LactateDehydrogenase&diff=55297MiPNet08.18 LactateDehydrogenase2013-12-02T08:43:24Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{Publication<br />
|title=Kuznetsov AV, Gnaiger E. Laboratory protocol: Lactate dehydrogenase. Cytosolic marker enzyme. Mitochondr Physiol Network 08.18.<br />
|info=[[Media:MiPNet08.18 LDH.pdf|'''Open Access-MiPNet08.18.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet08.18_LDH.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=2003<br />
|journal=Mitochondr Physiol Network<br />
|abstract='''O2k-Protocol for Lactate Dehydrogenase Cytosolic Marker Enzyme '''<br />
<br />
Lactate dehydrogenase (EC 1.1.1.27) is an enzyme, which catalyzes the last step in glycolysis. LDH is a soluble enzyme and localized in the cytosol (cytoplasm). LDH, therefore, is used as a quantitative marker enzyme for the intact cell, its activity providing information on cellular glycolytic capacity (Renner et al, 2003). Measurement of LDH release (leakage) is an important and frequently applied test for cellular membrane permeabilization (rupture) and severe irreversible cell damage. LDH leakage normally correlates well with CK release and the trypan blue viability test.<br />
<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
|keywords=Enzymes<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
{{Labeling<br />
|area=Instruments;methods<br />
|preparations=Enzyme<br />
|enzymes=Marker Enzyme<br />
|instruments=Protocol<br />
|additional=Enzymes<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
== Further information ==</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet08.13_mt-Isolation-RLM&diff=55295MiPNet08.13 mt-Isolation-RLM2013-12-02T08:39:13Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{Publication<br />
|title=Lassnig B, Gnaiger E. Laboratory protocol: Isolation of rat liver mitochondria. Mitochondr Physiol Network 08.13.<br />
|info=[[Media:MiPNet08.13 MitoIsolation-RLM.pdf|'''Open Access-MiPNet08.13.pdf]]; [http://www.bioblast.at/index.php/File:MiPNet08.13_MitoIsolation-RLM.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=2003<br />
|journal=Mitochondr Physiol Network<br />
|abstract='''O2k-Protocol for Isolation of Rat Liver Mitochondria'''<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
|discipline=Mitochondrial Physiology<br />
}}<br />
{{Labeling<br />
|organism=Rat<br />
|tissues=Liver<br />
|preparations=Isolated Mitochondria<br />
|instruments=Protocol<br />
|additional=mt-Preparation<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
|discipline=Mitochondrial Physiology<br />
}}<br />
== Further information ==<br />
<br />
:>> [http://bioblast.at/images/3/3c/MiPNet03.02_Chemicals-Media.pdf Chemicals and Media]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet08.12_IOC22&diff=55294MiPNet08.12 IOC222013-12-02T08:37:54Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{Publication<br />
|title=Gnaiger E, Doeller JE, Kraus D, Shiva S, Brookes PS, Darley-Usmar VM (2003) NO effect on mitochondrial oxygen kinetics at low oxygen. Oxygraph-2k workshop Report. Mitochondr Physiol Network 08.12.<br />
|info=[[Media:MiPNet08.12 NO-O2kWorkshop.pdf|'''Open Access-MiPNet08.12.pdf]]; [http://www.bioblast.at/index.php/File:MiPNet08.12_NO-O2kWorkshop.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=2003<br />
|journal=Mitochondr Physiol Network<br />
|abstract='''O2k-Protocol for NO effect on mitochondrial oxygen kinetics at low oxygen'''<br />
<br />
A single pilot experiment was carried out during an Oxygraph-2k workshop on high-resolution respirometry (IOC22). Respiration of isolated rat liver mitochondria was inhibited by addition of NO, which increased the sensitivity to oxygen >25-fold when compared to the half-saturation oxygen pressure, p50, in the absence of NO. Oxygen kinetics followed a monophasic hyperbolic function up to 2.2 kPa with NO (p50=0.93 kPa), compared to the standard oxygen range to 1.1 kPa without NO (p50=0.035 kPa).<br />
<br />
[[Image:MiPNet08.12.jpg|400px|centre|thumb|Figure 1. Oxygen dependence of mt-respiration and competitive inhibition by NO. The full line shows oxygen kinetics at state 3 with pyruvate and malate in the absence of NO, measured in the physiological oxygen range (from Gnaiger et al. 1998a). Dotted lines show inhibition of respiration by the indicated NO concentrations, where measurements were performed with low-resoltion respirometry and are restricted to the high oxygen range (from Koivisto et al. 1977). Extrapolations into the physiological oxygen range (shaded region) suggest sigmoidal oxygen kinetics, which requires testing by direct measurements at low oxygen (modified after Gnaiger, Kuznetsov 2002).]]<br />
<br />
[http://www.bioblast.at/index.php/Aguirre_2010_Biochim_Biophys_Acta Reference: Biochim Biophys Acta 1797: 557-565 (2010) ]<br />
<br />
See also: [http://www.oroboros.at/index.php?id=no-oxygen Combined O2 and NO measurement and oxygen kinetics]<br />
<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
|mipnetlab=AT_Innsbruck_OROBOROS, US_AL Birmingham_Kraus DW<br />
}}<br />
{{Labeling<br />
|area=Respiration, Instruments;methods<br />
|organism=Rat<br />
|tissues=Liver<br />
|preparations=Isolated Mitochondria<br />
|injuries=Hypoxia, RONS; Oxidative Stress<br />
|topics=Inhibitor, O2<br />
|couplingstates=OXPHOS<br />
|instruments=Oxygraph-2k, NO, Protocol<br />
|additional=O2k-Demo<br />
}}<br />
== Further information ==<br />
<br />
<br />
* Version 7: 2011-12-11<br />
* Version 1: 2003<br />
<br />
<br />
<br />
[[Category:OroboPedia]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet08.12_IOC22&diff=55293MiPNet08.12 IOC222013-12-02T08:37:36Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{Publication<br />
|title=Gnaiger E, Doeller JE, Kraus D, Shiva S, Brookes PS, Darley-Usmar VM (2003) NO effect on mitochondrial oxygen kinetics at low oxygen. Oxygraph-2k workshop Report. Mitochondr Physiol Network 08.12.<br />
|info=[[Media:MiPNet08.12 NO-O2kWorkshop.pdf|'''Open Access-MiPNet08.12.pdf]]; [http://www.bioblast.at/index.php/File:MiPNet08.12_NO-O2kWorkshop.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=2003<br />
|journal=Mitochondr Physiol Network<br />
|abstract='''O2k-Protocol for NO effect on mitochondrial oxygen kinetics at low oxygen'''<br />
<br />
A single pilot experiment was carried out during an Oxygraph-2k workshop on high-resolution respirometry (IOC22). Respiration of isolated rat liver mitochondria was inhibited by addition of NO, which increased the sensitivity to oxygen >25-fold when compared to the half-saturation oxygen pressure, p50, in the absence of NO. Oxygen kinetics followed a monophasic hyperbolic function up to 2.2 kPa with NO (p50=0.93 kPa), compared to the standard oxygen range to 1.1 kPa without NO (p50=0.035 kPa).<br />
<br />
[[Image:MiPNet08.12.jpg|300px|centre|thumb|Figure 1. Oxygen dependence of mt-respiration and competitive inhibition by NO. The full line shows oxygen kinetics at state 3 with pyruvate and malate in the absence of NO, measured in the physiological oxygen range (from Gnaiger et al. 1998a). Dotted lines show inhibition of respiration by the indicated NO concentrations, where measurements were performed with low-resoltion respirometry and are restricted to the high oxygen range (from Koivisto et al. 1977). Extrapolations into the physiological oxygen range (shaded region) suggest sigmoidal oxygen kinetics, which requires testing by direct measurements at low oxygen (modified after Gnaiger, Kuznetsov 2002).]]<br />
<br />
[http://www.bioblast.at/index.php/Aguirre_2010_Biochim_Biophys_Acta Reference: Biochim Biophys Acta 1797: 557-565 (2010) ]<br />
<br />
See also: [http://www.oroboros.at/index.php?id=no-oxygen Combined O2 and NO measurement and oxygen kinetics]<br />
<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
|mipnetlab=AT_Innsbruck_OROBOROS, US_AL Birmingham_Kraus DW<br />
}}<br />
{{Labeling<br />
|area=Respiration, Instruments;methods<br />
|organism=Rat<br />
|tissues=Liver<br />
|preparations=Isolated Mitochondria<br />
|injuries=Hypoxia, RONS; Oxidative Stress<br />
|topics=Inhibitor, O2<br />
|couplingstates=OXPHOS<br />
|instruments=Oxygraph-2k, NO, Protocol<br />
|additional=O2k-Demo<br />
}}<br />
== Further information ==<br />
<br />
<br />
* Version 7: 2011-12-11<br />
* Version 1: 2003<br />
<br />
<br />
<br />
[[Category:OroboPedia]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet06.06_Chemical_O2_background&diff=55292MiPNet06.06 Chemical O2 background2013-12-02T08:36:39Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{Publication<br />
|title=Kuznetsov AV, Gnaiger E (2001) Oxygraph assay of cytochrome c oxidase activity: Chemical background correction. Mitochondr Physiol Network 06.06.<br />
|info=[[Media:MiPNet06.06 ChemicalBackground.pdf|'''Open Access-MiPNet06.06''']]; [http://www.bioblast.at/index.php/File:MiPNet06.06_ChemicalBackground.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=2001<br />
|journal=Mitochondr Physiol Network<br />
|abstract=Autoxidation of reduced compounds (such as ascorbate, TMPD, cytochrome c) causes a chemical background oxygen flux, which is a function of oxygen concentration and has to be subtracted fom total oxygen flux. The new DatLab software provides on-line correction for instrumental and chemical background.<br />
<table><br />
<tr><br />
<td>[[Image:MiPNet06.06_Fig1.jpg|center|400px|thumb]]</td><br />
<td>[[Image:MiPNet06.06_Fig2.jpg|center|400px|thumb]]</td><br />
</tr><br />
<tr><br />
<td><span style="color:grey">Fig. 1. Continuous records of chemical background flux with 0.5 mM TMPD, 2 mM ascorbate,<br> and various cytochrome c concentrations (μM, indicated as numbers at each curve), plotted as a <br> function of oxygen concentration. Measured at 30 °C in mitochondrial medium R05 with 280 IU/ml <br> catalase. The linear sections of the curves were used for background corrections at high oxygen >50 μM.</span></td><br />
<td align:right><span style="color:grey; align:right">Fig. 2. Parameters of chemical background oxygen flux with TMPD+ascorbate as a <br> function of cytochrome c concentration (Cyt c [μM). ao’ intercept and bo’ slope of <br>linear part of oxygen dependence, from Fig.1 </span></td><br />
</tr><br />
</table><br />
<br />
<br />
<br />
'''O2k-Protocol for chemical background correction '''<br />
<br />
Oxygraphic determination of cytochrome c oxidase activity in the presence of TMPD, ascorbate and cytochrome c requires consideration of chemical background oxygen consumption. Several compounds are readily oxidized by molecular oxygen when dissolved in water. This leads to a significant chemical oxygen consumption in the absence of any respiring biological sample. The rate of this autoxidation represents a chemical background which strongly depends on experimental conditions, such as temperature, chemical composition, pH and oxygen concentration. Moreover, the rate of autoxidation may be significantly catalyzed by metal traces and metal-containing proteins (e.g. cytochrome c). Therefore, high-resolution respirometric measurement of oxygen flux ultimately depends on chemical background correction analyzed under experimental conditions over the entire oxygen range.<br />
<br />
After sequential titration of ascorbate (added first) and TMPD (with or without added cytochrome c), the total oxygen flux increases due to (i) COX activity and (ii) autooxidation of ascrobate and TMPD. Correction for autooxidation is a routine procedure in high-resolution respirometry, but it requires precautions. We recommend, therefore, evaluation of chemical background effects by application of inhibitors of cytochrome c oxidase (cyanide, azide) subsequent to recording of flux with ascorbate and TMPD. After full inhibitioin of COX, a small non-COX component of biological oxidations and the large effect of autooxidation are recorded over a range of further declining oxygen concentration. After re-oxygenation, inhibited oxygen flux is recorded at oxygen levels above and within the oxygen concentration pertaining to the COX activity measurment. This provides a complete internal calibration of the chemical background, as a function of oxygen concentration.<br />
<br />
<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
}}<br />
{{Labeling<br />
|area=Instruments;methods<br />
|preparations=Enzyme, Oxidase; Biochemical Oxidation<br />
|instruments=Oxygraph-2k, Protocol<br />
|additional=O2k-SOP<br />
}}<br />
== Further information ==<br />
'''References'''<br />
<br />
[[Renner_2002_Mol_Biol_Rep| Renner K, Kofler R, Gnaiger E (2002) Mitochondrial function in glucocorticoid triggered T-ALL cells with transgenic Bcl-2 expression. Mol Biol Rep 29: 97-101.]]<br />
<br />
<br />
* Version 7: 2010-08-23<br />
* Version 1: 2001<br />
<br />
<br />
<br />
<br />
[[Category:OroboPedia]]</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet06.03_POS-calibration-SOP&diff=55289MiPNet06.03 POS-calibration-SOP2013-12-02T07:49:18Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{Publication<br />
|title=Gnaiger E (2001) Oxygen calibration and solubility in experimental media. Mitochondr Physiol Network 06.03.<br />
|info=[[Media:MiPNet06.03 O2-Calibration-Solubility.pdf|'''Open Access-MiPNet06.03.pdf]]; [http://www.bioblast.at/index.php/File:MiPNet06.03_O2-Calibration-Solubility.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=2001<br />
|journal=Mitochondr Physiol Network<br />
|abstract='''O2k-Protocol for oxygen calibration and solubility'''<br />
<br />
[[High-resolution respirometry]] critically depends on accurate calibration of the [[polarographic oxygen sensor]]s which respond to [[Oxygen_pressure| partial oxygen pressure]]. Expressing the oxygen signal in terms of partial pressure has several advantages, but respiration is expressed in molar units related to biochemical stoichiometries. Conversion of oxygen partial pressure to [[oxygen concentration]] requires accurate information on oxygen solubilities in experimental incubation media. Absolute errors up to 10% are commonly found in the established literature. The [[oxygen solubility]] of mitochondrial respiration medium [[MiR06]] relative to pure water (oxygen solubility factor, ''F''<sub>M</sub>) is 0.92, accurately determined (for MiR05, i.e. MiR06 without catalase; [[MiPNet14.13]]) at 37 °C and 30 °C. At air saturation at standard barometric pressure (100 kPa) and 37 °C, the partial oxygen pressure is 19.63 kPa, and the oxygen concentration is 190.7 µM in MiR06. A clean and ready-to-use Oxygraph chamber is a requirement for saving time and quickly calibrating the polarographic oxygen sensors.<br />
<br />
<br />
See also: Oxygen solubility factor and equilibrium concentration of mitochondrial respiration medium [http://bioblast.at/images/d/d9/MiPNet14.13_Medium-MiR06.pdf MiR06] (i.e. MiR05 + catalase). <br />
<br />
[[Image:MiPNet06.03_Table.jpg|centre|frame|<sup>c</sup>O2* is the oxygen concentration at thermodynamic equilibrium with air under standard conditions of 100 kPa barometric pressure. FM has been determined for MiR05 at 30 °C and 37 °C, and is assumed to be identical at 25 °C.]]<br />
<br />
<br />
'''References'''<br />
<br />
This article is cited in:<br />
<br />
[http://www.ncbi.nlm.nih.gov/pubmed/18370948 Rodriguez JV, Pizarro MD, Scandizzi AL, Guibert EE, Almada LL, Mamprin ME (2008) Construction and performance of a minibioreactor suitable as experimental bioartificial liver. Artif Organs 32:323-328]<br />
<br />
[http://www.bioblast.at/images/8/82/Forstner_1983_POS.pdf Forstner H, Gnaiger E (1983) Calculation of equilibrium oxygen concentration. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York: 321-333.]<br />
<br />
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]<br />
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
}}<br />
{{Labeling<br />
|area=Instruments;methods<br />
|instruments=Oxygraph-2k, Protocol<br />
|additional=O2k-SOP<br />
}}<br />
== Further information ==<br />
:>> MiPNet11.08 integrated into MiPNet06.03.<br />
<br />
* Version 1: 2001<br />
* Version 11: 2011-12-11<br />
<br />
==Contents==<br />
<br />
1 O2k-Chamber: Cleaning and Storage<br />
<br />
1.1 Cleaning the Chamber<br />
<br />
1.2 Storage in 70% Ethanol<br />
<br />
2 Short Two-Point Calibration<br />
<br />
3 Equations for Oxygen Calibration<br />
<br />
3.1 Oxygen Concentration and Recorded Signal<br />
<br />
3.2 Oxygen Pressure and POS Current<br />
<br />
3.3 Oxygen Concentration and Oxygen Pressure<br />
<br />
3.4 Oxygen Signal and Background Oxygen Consumption<br />
<br />
4 O2 Solubility and Concentration at Air Saturation<br />
<br />
4.1 Oxygen Pressure and Concentration<br />
<br />
4.2 Temperature Effect on Saturation O2 Conc.<br />
<br />
4.3 Barometric Pressure Effect on Saturation O2 Conc.<br />
<br />
4.4 The O2 Solubility Factor in Salt Solutions<br />
<br />
<br />
__NOTOC__</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet04.05_Titration-Injection&diff=55288MiPNet04.05 Titration-Injection2013-12-02T07:22:02Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{Publication<br />
|title=Gnaiger E, Rieger G (1999) From step titration to ramp injection: Uncoupling by FCCP with TIP. Mitochondr Physiol Network 04.05.<br />
|info=[[Media:MiPNet04.05 Titration-Injection.pdf|'''Open Access-MiPNet04.05.pdf''']]; [http://www.oroboros.at/?tip2k www Tip2k]; [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=1999<br />
|journal=Mitochondr Physiol Network<br />
|abstract=Fully supported by the O2k-Core and control of the TIP2k by the software DatLab: The TIP2k can be programmed for multiple titrations and continuous injections. As an alternative to traditional step titration, the TIP offers the new option of ramp injection, providing maximum resolution of the concentration dependence of oxygen flux. This is illustrated by the recording of cellular respiratory flux as a function of a continuous increase of uncoupler (FCCP) concentration.<br />
<br />
''Titration:''<br />
Programmable, automatic titration regimes, with titration volumes of 0.05 to 250 µl, variable titration intervals and duration of titration pulses.<br />
<br />
<br />
''Injection:''<br />
Steady-state injection: Operation at quasi steady-states by continuous injection of substrates at limiting rates of consumption, providing new flexibility in experimental design by combining the technical advantages of closed and open systems. Programmable injection flows: 0.01 to 25 µl.s-1.<br />
Ramp injection (MiPNet04.05, see above): Ramp increase of effector concentrations by "continuous titration".<br />
DatLab software for feedback control by the the TIP2k: for steady-state respirometry at selected oxygen levels and pH-stat applications.<br />
<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
{{Labeling<br />
|area=Respiration, Instruments;methods<br />
|organism=Human<br />
|tissues=Endothelial; Epithelial; Mesothelial Cell<br />
|model cell lines=HUVEC<br />
|preparations=Intact cells<br />
|topics=Uncoupler<br />
|instruments=Oxygraph-2k, TIP2k, Protocol<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet04.05_Titration-Injection&diff=55287MiPNet04.05 Titration-Injection2013-12-02T07:21:38Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{Publication<br />
|title=Gnaiger E, Rieger G (1999) From step titration to ramp injection: Uncoupling by FCCP with TIP. Mitochondr Physiol Network 04.05.<br />
|info=[[Media:MiPNet04.05 Titration-Injection.pdf|'''Open Access-MiPNet04.05.pdf''']]; [http://www.oroboros.at/?tip2k www Tip2k]; [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=1999<br />
|journal=Mitochondr Physiol Network<br />
|abstract=Fully supported by the O2k-Core and control of the TIP2k by the software DatLab: The TIP2k can be programmed for multiple titrations and continuous injections. As an alternative to traditional step titration, the TIP offers the new option of ramp injection, providing maximum resolution of the concentration dependence of oxygen flux. This is illustrated by the recording of cellular respiratory flux as a function of a continuous increase of uncoupler (FCCP) concentration.<br />
<br />
''Titration''<br />
Programmable, automatic titration regimes, with titration volumes of 0.05 to 250 µl, variable titration intervals and duration of titration pulses.<br />
<br />
<br />
''Injection''<br />
Steady-state injection: Operation at quasi steady-states by continuous injection of substrates at limiting rates of consumption, providing new flexibility in experimental design by combining the technical advantages of closed and open systems. Programmable injection flows: 0.01 to 25 µl.s-1.<br />
Ramp injection (MiPNet04.05, see above): Ramp increase of effector concentrations by "continuous titration".<br />
DatLab software for feedback control by the the TIP2k: for steady-state respirometry at selected oxygen levels and pH-stat applications.<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
{{Labeling<br />
|area=Respiration, Instruments;methods<br />
|organism=Human<br />
|tissues=Endothelial; Epithelial; Mesothelial Cell<br />
|model cell lines=HUVEC<br />
|preparations=Intact cells<br />
|topics=Uncoupler<br />
|instruments=Oxygraph-2k, TIP2k, Protocol<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}</div>Bidaurratzaga Eiderhttps://wiki.oroboros.at/index.php?title=MiPNet04.05_Titration-Injection&diff=55286MiPNet04.05 Titration-Injection2013-12-02T07:20:40Z<p>Bidaurratzaga Eider: </p>
<hr />
<div>{{Publication<br />
|title=Gnaiger E, Rieger G (1999) From step titration to ramp injection: Uncoupling by FCCP with TIP. Mitochondr Physiol Network 04.05.<br />
|info=[[Media:MiPNet04.05 Titration-Injection.pdf|'''Open Access-MiPNet04.05.pdf''']]; [http://www.oroboros.at/?tip2k www Tip2k]; [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=1999<br />
|journal=Mitochondr Physiol Network<br />
|abstract=Fully supported by the O2k-Core and control of the TIP2k by the software DatLab: The TIP2k can be programmed for multiple titrations and continuous injections. As an alternative to traditional step titration, the TIP offers the new option of ramp injection, providing maximum resolution of the concentration dependence of oxygen flux. This is illustrated by the recording of cellular respiratory flux as a function of a continuous increase of uncoupler (FCCP) concentration.<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
{{Labeling<br />
|area=Respiration, Instruments;methods<br />
|organism=Human<br />
|tissues=Endothelial; Epithelial; Mesothelial Cell<br />
|model cell lines=HUVEC<br />
|preparations=Intact cells<br />
|topics=Uncoupler<br />
|instruments=Oxygraph-2k, TIP2k, Protocol<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}</div>Bidaurratzaga Eider