https://wiki.oroboros.at/api.php?action=feedcontributions&user=Bauer+Christiane&feedformat=atomBioblast - User contributions [en]2024-03-28T10:35:29ZUser contributionsMediaWiki 1.36.1https://wiki.oroboros.at/index.php?title=BAM15&diff=61765BAM152014-05-21T13:00:40Z<p>Bauer Christiane: </p>
<hr />
<div>{{MitoPedia<br />
|abbr=DNP<br />
|description='''2,4-dinitrophenol''' (C<sub>6</sub>H<sub>4</sub>N<sub>2</sub>O<sub>5</sub>; FW = 184.11) is a protonophore acting as an [[uncoupler]] of [[Oxidative phosphorylation|oxidative phosphorylation]].<br />
|info=[[Steinlechner-Maran 1996 Am J Physiol Cell Physiol]]<br />
|type=Uncoupler<br />
}}<br />
{{MitoPedia methods|type=Uncoupler<br />
}}<br />
{{MitoPedia topics<br />
|mitopedia topic=Uncoupler<br />
|type=Uncoupler<br />
}}<br />
'''Preparation of 10 mM DNP stock solution''' (dissolved in H<sub>2</sub>O):<br />
<br />
<span style="color:#8B008B"> '''Caution:''' Toxic!</span><br />
<br />
::1. Weigh 3.7 mg of DNP (FW = 184.11).<br />
::2. Dissolve in 1.2 ml H2O.<br />
::3. Neutralize with 1 N KOH.<br />
::4. Adjust final volume to 2 ml and divide into 0.2 ml portions.<br />
::6. Store at -20 °C.</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=Talk:BAM15&diff=61764Talk:BAM152014-05-21T12:59:21Z<p>Bauer Christiane: Created page with "'''2-fluorophenyl){6-[(2-fluorophenyl)amino](1,2,5-oxadiazolo[3,4-e]pyrazin-5-yl)}amine''' is a protonophore or uncoupler of oxidative phosphorylation detected in a screen fo..."</p>
<hr />
<div>'''2-fluorophenyl){6-[(2-fluorophenyl)amino](1,2,5-oxadiazolo[3,4-e]pyrazin-5-yl)}amine''' is a protonophore or [[uncoupler]] of oxidative phosphorylation detected in a screen for uncoupling agents exerting less toxicity than commonly used uncouplers and first described by [[Kennwood 2013 Mol MeTAB| Kennwood et al. 2013]].<br />
In their comparative study using BAM 15 and [[FCCP]] it was shown to increase oxygen flux to a similar extent as the classical uncoupler, to display a much broader range of concentrations inducing maximum respiration, to elicit no formation of H<sub>2</sub>O<sub>2</sub>, to leave cellular membrane potential unaffected, and to ultimately exert less cytotoxicity.</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=BAM15&diff=61763BAM152014-05-21T12:59:15Z<p>Bauer Christiane: </p>
<hr />
<div><br />
<br />
{{MitoPedia<br />
|abbr=DNP<br />
|description='''2,4-dinitrophenol''' (C<sub>6</sub>H<sub>4</sub>N<sub>2</sub>O<sub>5</sub>; FW = 184.11) is a protonophore acting as an [[uncoupler]] of [[Oxidative phosphorylation|oxidative phosphorylation]].<br />
|info=[[Steinlechner-Maran 1996 Am J Physiol Cell Physiol]]<br />
|type=Uncoupler<br />
}}<br />
{{MitoPedia methods|type=Uncoupler<br />
}}<br />
{{MitoPedia topics<br />
|mitopedia topic=Uncoupler<br />
|type=Uncoupler<br />
}}<br />
'''Preparation of 10 mM DNP stock solution''' (dissolved in H<sub>2</sub>O):<br />
<br />
<span style="color:#8B008B"> '''Caution:''' Toxic!</span><br />
<br />
::1. Weigh 3.7 mg of DNP (FW = 184.11).<br />
::2. Dissolve in 1.2 ml H2O.<br />
::3. Neutralize with 1 N KOH.<br />
::4. Adjust final volume to 2 ml and divide into 0.2 ml portions.<br />
::6. Store at -20 °C.</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=Zhang_Xiaolei&diff=59638Zhang Xiaolei2014-05-07T11:41:09Z<p>Bauer Christiane: Created page with "{{Person |lastname=Zhang |firstname=Xiaolei |institution=Translational Research Institute for Metabolism and Diabetes Florida Hospital Sanford/Burnham Institute |address=301 E..."</p>
<hr />
<div>{{Person<br />
|lastname=Zhang<br />
|firstname=Xiaolei<br />
|institution=Translational Research Institute for Metabolism and Diabetes<br />
<br />
Florida Hospital<br />
<br />
Sanford/Burnham Institute <br />
|address=301 E Princeton St<br />
<br />
|area code=32804<br />
|city=Orlando <br />
|state=Florida (FL)<br />
|country=US<br />
|mailaddress=Xiaolei.Zhang@flhosp.org<br />
}}<br />
{{Labelingperson}}</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MiP2014&diff=59419MiP20142014-05-06T11:47:49Z<p>Bauer Christiane: /* MiP2014 preliminary list of participants */</p>
<hr />
<div>[[Image:IUBMB-logo.gif|left|100px|link=http://www.iubmb.org|IUBMB - ''International Union of Biochemistry and Molecular Biology'']]<br />
[[Image:MiPsocietyLOGO.JPG|right|200px|link=http://www.mitophysiology.org|MiPsociety]]<br />
[http://www.mitophysiology.org/?MiP2014 '''<big>MiP2014 - 10<sup>th</sup> MiP''conference'': Joint IUBMB/MiP Symposium on Mitochondrial Physiology - a Point/Counterpoint Meeting</big>''']<br />
<br />
'''''International Union of Biochemistry and Molecular Biology''''' and '''Mitochondrial Physiology Society'''<br />
<br />
Obergurgl, Tyrol, Austria. 2014-Sep-08 to 12<br />
== MiP2014 - a point/counterpoint meeting ==<br />
<br />
Mitochondrial research has recently undergone a dramatic renaissance, due to numerous discoveries revealing its central role in cell death, disease pathology, aging, thermogenesis, oxidative stress, cell signalling, cellular regulation and cell bioenergetics. The aim of the small symposium is to bring together experts in mitochondrial research spanning from the biochemical and molecular level to the interactions within the cell. In addition to the latest scientific findings, new methodological developments in measuring mitochondrial function and dysfunction are presented. A point/counterpoint format is chosen to discuss selected hot topics and some fundamental methodological and conceptual issues.<br />
<br />
The expected attendance is max. 100 participants. ->> Compare [[MiP2013 Programme]]<br />
Abstracts and presentations: refer to [http://www.chem.qmul.ac.uk/iubmb/ IUBMB nomenclature]; >> [[MitoPedia Glossary: Respiratory states|MitoPedia Glossary: '''Respiratory states''']]<br />
<br />
== Programme structure ==<br />
<br />
In each of the 12 sessions a point/counterpoint introduction will be given by invited keynote speakers to provide a balanced overview and promote discussions. Short oral presentations are selected from abstracts, including student presentations. Rapid platform statements will introduce posters which contribute to a specific session. Posters are up throughout the meeting, and poster discussions are integrated into the platform sessions.<br />
<br />
=== A. Mitochondrial respiratory control === <br />
* A1. mtDNA, mt-phenotype and disease – is there biochemical and molecular-genetic evidence to explain observed associations? <br />
* A2. Fission and fusion, mitochondrial structure and function - is there a tight connection? <br />
* A3. Mitochondrial density and normalization of respiration – is there a general biochemical or molecular marker? <br />
* A4. Biochemical reserve capacity, respiratory control ratios and coupling efficiency – are conventional concepts inconsistent?<br />
<br />
=== B. Experimental protocols in mitochondrial biochemistry === <br />
* B1. Incubation media for mt-preparations and experimental temperature in functional studies of mammalian mitochondria – guided by concept or tradition? <br />
* B2. Intracellular oxygen pressure versus oxygen regimes in studies of ROS production and respiration - are mitochondria sources or sinks of ROS?<br />
* B3. Quantitative measurement of mt-membrane potential - potentiometric/fluorometric. <br />
* B4. Instrumental platforms in mitochondrial physiology – industrial presentations.<br />
<br />
=== C. Towards quality assurance === <br />
* C1. Do blood cells versus muscle biopsies provide valid models for diagnosis of mitochondrial pathologies? <br />
* C2. Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
* C3. From availability and quality control of chemicals to mitochondrial respiratory protocols – who controls? <br />
* C4. Standards for the diagnosis of mitochondrial pathologies – a challenge for collaboration with mitochondrial organizations?<br />
Particularly in view of Subtopic C, it may be attractive for mitochondrial organizations to send and support delegates. The conference will, therefore, provide a unique forum for international networking, and may inspire new national groups to be formed in contact with established mitochondrial organizations:<br />
>> '''[[MitoGlobal_Organizations]]'''<br />
<br />
== MiP2014 preliminary list of participants ==<br />
<br />
in progress<br />
<br />
see http://www.mitophysiology.org/?participants<br />
<br />
== Sponsors ==<br />
<br />
[[Image:OROBOROS Oxygraph-2k Logo.jpg|left|200px|link=http://www.oroboros.at|OROBOROS]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[Image:HAMLogoRGB.jpg|left|200px|link=http://www.hamilton.ch|Hamilton]]<br />
<br />
<br />
<br />
<br />
<br />
[[Image:Hansatech.jpg|left|200px|link=http://www.hansatech-instruments.com/|Hansatech]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
== Venue ==<br />
<br />
The symposium will be hosted in the '''University Center in Obergurgl''' 100 km from Innsbruck. The closest airport is in Innsbruck. Participants arriving at Munich airport will take the train or an airport shuttle to Innsbruck. We provide support in arranging the transfer between Munich and Innsbruck. A bus transfer is organized between Innsbruck and Obergurgl.<br />
<br />
The Obergurgl University Center is located at an altitude of 1940 meters above sea-level. The historical buildings, carefully refurbished and equipped with the latest technical systems, can accommodate around 70 persons in 43 bedrooms and includes conference rooms, the Alpine Research Center, laboratory, sports medicine, lounges and the restaurant. All rooms are equipped with an Internet connection. Additional participants are accommodated in smaller guest houses in 5 minutes walking distance. Meals are served for all participants in the University Center. Two conference rooms for a total of 200 persons and three small rooms for round table discussions are available. There is enough space for poster presentations and instrument exhibitions. The cosy bar and lounge area with an open fire place adds to the familiar atmosphere and supports the interactive spirit of the symposium.<br />
<br />
The small village of Obergurgl is situated in an impressive landscape at about 1900 m above sea level in the Ötz valley. It is surrounded by mountains, offering an exceptional view to the Ötz valley glaciers. During summer time the area is famous for hiking and for skiing in winter time. On the doorstep: one of Europe´s finest Biosphere Parks. The unique surroundings offer the possibility of combining scientific discussions while exploring the alpine environment during the famous “walks and talks”. The informal atmosphere of the workshop encourages also students and young post-docs to participate actively in scientific discussions and to develop future collaborations.<br />
<br />
University Centre Obergurgl, Gaisbergweg 5, 6456 Obergurgl, Tyrol, Austria.<br />
Tel.: +43 (0) 512 507 37201 | Fax: +43 (0) 512 507 37400 | Email: obergurgl@uibk.ac.at<br />
<br />
== International programme committee ==<br />
The international programme committee will provide scientific input by active participation, nominate further invited speakers to ensure balanced point/counterpoint introductions, and select the abstracts as a basis for participation (restricted number of participants), for oral 10+5 min presentations, and for flash presentations.<br />
<br />
Chair:<br />
* [[Gnaiger E|Gnaiger Erich]], Medical University Innsbruck, AT. - erich.gnaiger@mitophysiology.org<br />
Members:<br />
* [[Ali SS|Ali Sameh S]], Helmy Institute of Medical Sciences, Giza, Egypt<br />
* [[Borutaite V|Borutaite Vilma]], Lithuanian University of Health Sciences Kaunas, LT<br />
* [[Neurocon|Chakrabarti Sasanka]], Institute of Post-graduate Medical Education & Research, Kolkatta, India<br />
* [[Chinese Society of Mitochondrial Research and Medicine|Chen Quan]], Chinese Academy of Sciences, Nankai University, Beijing <br />
* [[Dela F|Dela Flemming]], University of Copenhagen, DK <br />
* [[Duchen MR|Duchen Michael R]], University College London, UK<br />
* [[Elmer E|Elmér Eskil]], Lund University, SE <br />
* [[Hand SC|Hand Steven]], Louisiana State University, LA, US<br />
* [[Hickey AJ|Hickey Anthony J]], University of Auckland, NZ<br />
* [[Koopman WJ|Koopman Werner J]], Radboud Univ Nijmegen Medical Centre, NL<br />
* [[Tanaka M|Tanaka Masashi]], Toyko Metropolitan Institute of Gerontology, JP<br />
* [[Neufer PD|Neufer P Darrell]], East Carolina University, Greenville, NC, US<br />
* [[Ojuka E|Ojuka Edward]], University of Cape Town, South Africa<br />
* [[Pecina P|Pecina Petr]], Academy of Sciences of the Czech Republic, CZ<br />
* [[Perry CG|Perry Christopher G]], York University, Toronto, Canada<br />
* [[Rossignol R|Rossignol Rodrigue]], Université Victor Segalen-Bordeaux, FR<br />
* [[Vercesi AE|Vercesi Anibal E]], State University of Campinas, Sao Paulo, Brazil<br />
* [[Taiwan Society for Mitochondrial Research and Medicine|Wei Yau-Huei]], Mackay Medical College, New Taipei City, Taiwan<br />
* [[Zorzano A|Zorzano Antonio]], Universitat de Barcelona, Spain<br />
<br />
== Information for invited speakers==<br />
<br />
MiP2014 will be held at the University Centre Obergurgl, with maximum capacity of 124 persons and a price per person per night of € 65.- including a shared double room, breakfast, and dinner. MiP''conferences'' are organized as small meetings facilitating communication between senior and junior participants. In the tradition of MiP''conferences'', we are asking invited speakers to cooperate and try to receive local support for joining us at the prestigious Mitochondrial Physiology Conference (limited to 124 participants). Support will be provided to students and young investigators.<br />
<br />
== Post-conference O2k-Workshop ==<br />
<br />
A [http://www.oroboros.at/?IOC_2014-09_obergurgl post-conference workshop] is organized on the same venue, for hands-on training in mitochondrial respiratory physiology, introducing the combined measurement of high-resolution respirometry and fluorometry (OROBOROS INSTRUMENTS). Since participants come from all-over-the-world, including many students, the combination of the symposium with the workshop makes travelling more economical for the maximum of 24 workshop-participants. The post-conference workshop is financially completely separate from the symposium.<br />
<br />
[[File:MitoGlobal.jpg|left|40px|MitoGlobal]]<br />
== MiP''events'' ==<br />
are listed as '''[[MitoGlobal_Events]]'''.<br />
<br />
<br />
<br />
<br />
<br />
[[Category:MitoPedia]]<br />
[[Category:All]]</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MiP2014&diff=59360MiP20142014-05-05T08:10:27Z<p>Bauer Christiane: /* MiP2014 preliminary list of participants */</p>
<hr />
<div>[[Image:IUBMB-logo.gif|left|100px|link=http://www.iubmb.org|IUBMB - ''International Union of Biochemistry and Molecular Biology'']]<br />
[[Image:MiPsocietyLOGO.JPG|right|200px|link=http://www.mitophysiology.org|MiPsociety]]<br />
[http://www.mitophysiology.org/?MiP2014 '''<big>MiP2014 - 10<sup>th</sup> MiP''conference'': Joint IUBMB/MiP Symposium on Mitochondrial Physiology - a Point/Counterpoint Meeting</big>''']<br />
<br />
'''''International Union of Biochemistry and Molecular Biology''''' and '''Mitochondrial Physiology Society'''<br />
<br />
Obergurgl, Tyrol, Austria. 2014-Sep-08 to 12<br />
== MiP2014 - a point/counterpoint meeting ==<br />
<br />
Mitochondrial research has recently undergone a dramatic renaissance, due to numerous discoveries revealing its central role in cell death, disease pathology, aging, thermogenesis, oxidative stress, cell signalling, cellular regulation and cell bioenergetics. The aim of the small symposium is to bring together experts in mitochondrial research spanning from the biochemical and molecular level to the interactions within the cell. In addition to the latest scientific findings, new methodological developments in measuring mitochondrial function and dysfunction are presented. A point/counterpoint format is chosen to discuss selected hot topics and some fundamental methodological and conceptual issues.<br />
<br />
The expected attendance is max. 100 participants. ->> Compare [[MiP2013 Programme]]<br />
Abstracts and presentations: refer to [http://www.chem.qmul.ac.uk/iubmb/ IUBMB nomenclature]; >> [[MitoPedia Glossary: Respiratory states|MitoPedia Glossary: '''Respiratory states''']]<br />
<br />
== Programme structure ==<br />
<br />
In each of the 12 sessions a point/counterpoint introduction will be given by invited keynote speakers to provide a balanced overview and promote discussions. Short oral presentations are selected from abstracts, including student presentations. Rapid platform statements will introduce posters which contribute to a specific session. Posters are up throughout the meeting, and poster discussions are integrated into the platform sessions.<br />
<br />
=== A. Mitochondrial respiratory control === <br />
* A1. mtDNA, mt-phenotype and disease – is there biochemical and molecular-genetic evidence to explain observed associations? <br />
* A2. Fission and fusion, mitochondrial structure and function - is there a tight connection? <br />
* A3. Mitochondrial density and normalization of respiration – is there a general biochemical or molecular marker? <br />
* A4. Biochemical reserve capacity, respiratory control ratios and coupling efficiency – are conventional concepts inconsistent?<br />
<br />
=== B. Experimental protocols in mitochondrial biochemistry === <br />
* B1. Incubation media for mt-preparations and experimental temperature in functional studies of mammalian mitochondria – guided by concept or tradition? <br />
* B2. Intracellular oxygen pressure versus oxygen regimes in studies of ROS production and respiration - are mitochondria sources or sinks of ROS?<br />
* B3. Quantitative measurement of mt-membrane potential - potentiometric/fluorometric. <br />
* B4. Instrumental platforms in mitochondrial physiology – industrial presentations.<br />
<br />
=== C. Towards quality assurance === <br />
* C1. Do blood cells versus muscle biopsies provide valid models for diagnosis of mitochondrial pathologies? <br />
* C2. Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
* C3. From availability and quality control of chemicals to mitochondrial respiratory protocols – who controls? <br />
* C4. Standards for the diagnosis of mitochondrial pathologies – a challenge for collaboration with mitochondrial organizations?<br />
Particularly in view of Subtopic C, it may be attractive for mitochondrial organizations to send and support delegates. The conference will, therefore, provide a unique forum for international networking, and may inspire new national groups to be formed in contact with established mitochondrial organizations:<br />
>> '''[[MitoGlobal_Organizations]]'''<br />
<br />
== MiP2014 preliminary list of participants ==<br />
<br />
in progress<br />
<br />
{| class="wikitable"<br />
|-<br />
! Name !! Institution !! Preliminary title <br />
|-<br />
| [[Ali SS|Ali Sameh S]]||Zewail City for Science and Technology, ET||Oxygen-consuming and ROS-producing activities in synaptosomes.<br />
|-<br />
|-<br />
| [[Amoedo ND|Amoedo Nivea D.]]||Federal University of Rio de Janeiro, BR||Dissecting the role of mitocondria in metastasis.<br />
|-<br />
| [[Bauer CM|Bauer Christiane Maria]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Borutaite_V|Borutaite Vilmante]]||Lithuanian University of Health Sciences, Kaunas, LT||Controversies in testing integrity of mitochondrial membranes.<br />
|-<br />
| [[Brown D|Brown David]]||East Carolina University, NC, US||<br />
|-<br />
| [[Contreras-Ferrat A|Contreras-Ferrat Ariel]]||University of Chile, CL||<br />
|-<br />
| [[Coen P|Coen Paul]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||Does calorie restriction improve or worsen skeletal muscle mitochondrial performance?<br />
|-<br />
| [[Dohlmann T|Dohlmann Tine]]||University of Copenhagen, DK||<br />
|-<br />
| [[Eggimann S|Eggimann Sandra]]||University Hospital Berne University, Bern, CH||<br />
|-<br />
| [[Elmer E|Elmer Eskil]]||Wallenberg Neuroscience Center, Lund, SE||Ex vivo respiratory capacity of human blood cells - a SUITable application of the O2k Oxygraph / A novel pharmacological approach to counteract metabolic crisis.<br />
|-<br />
| [[Felding-Habermann B|Felding-Habermann Brunhilde]]||The Scripps Research Institute, La Jolla, CA, US||Mitochondrial functions in breast cancer progression.<br />
|-<br />
| [[Goodpaster BH|Goodpaster Bret]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Gnaiger E|Gnaiger Erich]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Grefte S|Grefte Sander]]||Radboud University Nijmegen Medical Centre, Nijmegen, NL||Pathophysiology of mitochondrial (dys)function in skeletal muscle.<br />
|-<br />
| [[Hahn D|Hahn Dagmar]]||Inselspital Bern, CH||Validation of oxygen consumption measurements in muscle and fibroblasts from patients with mitochondrial diseases. <br />
|-<br />
| [[Hand SC|Hand Steven C]]||Louisiana State University, LA, US||Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
|-<br />
| [[Harrison DK|Harrison David K]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Hayashi A|Hayashi Akira]]||DDU Oncology, Takeda Pharmaceutical Company Ltd, Kanagawa, JP||<br />
|-<br />
| [[Hepple_RT| Hepple Russel T.]]||McGill University, Montreal, CA||Mitochondrial involvement in aging muscle: cause and effect(or).<br />
|-<br />
| [[Herminghaus A|Herminghaus Anna]]||Uniklinikum Düsseldorf, DE||Severity of polymicrobial sepsis modulates mitochondrial function in rat liver.<br />
|-<br />
| [[Hey-Mogensen M|Hey-Mogensen Martin]]||Novo Nordisk A/S Diabetes NBEs & Obesity Biology, Måløv, DK||<br />
|-<br />
| [[Jana Prado F|Jana Prado Fabian]]||University of Chile, CL||<br />
|-<br />
| [[Kandashvili M|Kandashvili Manana ]]||Tallinn University, EE||Regulation of energy metabolism in human colorectal cancer cells ''in situ''.<br />
|-<br />
| [[Kane DA| Kane Daniel A.]]||St. Francis Xavier University, Antigonish, CA||Mitochondrial shuttles in muscle.<br />
|-<br />
| [[Kraunsoe R|Kraunsoe Regitze]]||University of Copenhagen, DK||<br />
|-<br />
| [[Koopman WJ|Koopman Werner J]]||Radboud University Nijmegen Medical Centre, NL||Redox regulation of mitochondrial dynamics.<br />
|-<br />
| [[Kowaltowski AJ|Kowaltowski Alicia J.]]||University of Sao Paulo, BR||<br />
|-<br />
| [[Laner V|Laner Verena]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Larsen S|Larsen Steen]]||University of Copenhagen, DK||Normalization of respiration.<br />
|-<br />
| [[Larsson NG|Larsson Nils-Göran]]||Max Planck Institute for the Biology of Ageing, Cologne, DE||Mouse models with mtDNA mutations.<br />
|-<br />
| [[Ojuka E|Ojuka Edward]]||University of Cape Town, ZA||Fission and fusion, mitochondrial structure and function - is there a tight connection?<br />
|-<br />
| [[Perry CG|Perry Christopher G]]||York University, Ontario, CA||Re-visiting critical experimental parameters to control when measuring respiratory kinetics in permeabilized muscle fibres.<br />
|-<br />
| [[Pesta D|Pesta Dominik]]||Yale University School of Medicine, CT, US||Dysregulation of muscle ATP synthesis in hypophosphatemic NaPi2a knockout mice and in a patient with hypophosphatemia due to a mutation in NaPi2c. <br />
|- <br />
| [[Picard_M|Picard Martin]]||University of Pennsylvania, PA, US||Quantitative regulation of nuclear gene expression by mitochondrial DNA<br />
heteroplasmy.<br />
|-<br />
| [[Pecina P|Pecina Petr]]||Academy of Sciences of the Czech Republic, Prague, CZ||The use of lymphocytes for diagnostics of mitochondrial disorders.<br />
|-<br />
| [[Purhonen J|Purhonen Janne]]||University Helsinki, FI||<br />
|-<br />
| [[Ristow M|Ristow Michael]]||ETH Zurich, CH||Promoting health and lifespan by increasing mitochondrial ROS formation.<br />
|-<br />
| [[Shirihai O|Shirihai Orian]]||The Shirihai Lab at Boston University, MA, US||<br />
|-<br />
| [[Skulachev VP|Skulachev Vladimir]]||Lomonosov Moscow State University, Moscow, RU||Mitochondria are sources of ROS rather than sinks: Physiological effects of mitochondria-targeted antioxidants.<br />
|-<br />
| [[Smith S|Smith Steven]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Sparks LM|Sparks Lauren M]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||The potential role of mitochondrial efficiency and energetics in the endurance-trained phenotype of NAMPT Transgenic (Tg) mice.<br />
|-<br />
| [[Starkov AA|Starkov Anatoly]]||Weill Medical College Cornell University, NY, US||<br />
|-<br />
| [[Stiban J|Stiban Johnny]]||Birzeit University, Ramallah, PS||Very long chain ceramides interfere with C16-ceramide-induced channel formation: A plausible mechanism for regulating the initiation of intrinsic apoptosis.<br />
|-<br />
| [[Zorzano A|Zorzano Antonio]]||Universitat de Barcelona, ES||Metabolic implications of mitofusin 2 dysfunction.<br />
|-<br />
| [[Sumbalova Z|Sumbalova Zuzana]]||Comenius University, Bratislava, SK||<br />
|}<br />
<br />
== Sponsors ==<br />
<br />
[[Image:OROBOROS Oxygraph-2k Logo.jpg|left|200px|link=http://www.oroboros.at|OROBOROS]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[Image:HAMLogoRGB.jpg|left|200px|link=http://www.hamilton.ch|Hamilton]]<br />
<br />
<br />
<br />
<br />
<br />
[[Image:Hansatech.jpg|left|200px|link=http://www.hansatech-instruments.com/|Hansatech]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
== Venue ==<br />
<br />
The symposium will be hosted in the '''University Center in Obergurgl''' 100 km from Innsbruck. The closest airport is in Innsbruck. Participants arriving at Munich airport will take the train or an airport shuttle to Innsbruck. We provide support in arranging the transfer between Munich and Innsbruck. A bus transfer is organized between Innsbruck and Obergurgl.<br />
<br />
The Obergurgl University Center is located at an altitude of 1940 meters above sea-level. The historical buildings, carefully refurbished and equipped with the latest technical systems, can accommodate around 70 persons in 43 bedrooms and includes conference rooms, the Alpine Research Center, laboratory, sports medicine, lounges and the restaurant. All rooms are equipped with an Internet connection. Additional participants are accommodated in smaller guest houses in 5 minutes walking distance. Meals are served for all participants in the University Center. Two conference rooms for a total of 200 persons and three small rooms for round table discussions are available. There is enough space for poster presentations and instrument exhibitions. The cosy bar and lounge area with an open fire place adds to the familiar atmosphere and supports the interactive spirit of the symposium.<br />
<br />
The small village of Obergurgl is situated in an impressive landscape at about 1900 m above sea level in the Ötz valley. It is surrounded by mountains, offering an exceptional view to the Ötz valley glaciers. During summer time the area is famous for hiking and for skiing in winter time. On the doorstep: one of Europe´s finest Biosphere Parks. The unique surroundings offer the possibility of combining scientific discussions while exploring the alpine environment during the famous “walks and talks”. The informal atmosphere of the workshop encourages also students and young post-docs to participate actively in scientific discussions and to develop future collaborations.<br />
<br />
University Centre Obergurgl, Gaisbergweg 5, 6456 Obergurgl, Tyrol, Austria.<br />
Tel.: +43 (0) 512 507 37201 | Fax: +43 (0) 512 507 37400 | Email: obergurgl@uibk.ac.at<br />
<br />
== International programme committee ==<br />
The international programme committee will provide scientific input by active participation, nominate further invited speakers to ensure balanced point/counterpoint introductions, and select the abstracts as a basis for participation (restricted number of participants), for oral 10+5 min presentations, and for flash presentations.<br />
<br />
Chair:<br />
* [[Gnaiger E|Gnaiger Erich]], Medical University Innsbruck, AT. - erich.gnaiger@mitophysiology.org<br />
Members:<br />
* [[Ali SS|Ali Sameh S]], Helmy Institute of Medical Sciences, Giza, Egypt<br />
* [[Borutaite V|Borutaite Vilma]], Lithuanian University of Health Sciences Kaunas, LT<br />
* [[Neurocon|Chakrabarti Sasanka]], Institute of Post-graduate Medical Education & Research, Kolkatta, India<br />
* [[Chinese Society of Mitochondrial Research and Medicine|Chen Quan]], Chinese Academy of Sciences, Nankai University, Beijing <br />
* [[Dela F|Dela Flemming]], University of Copenhagen, DK <br />
* [[Duchen MR|Duchen Michael R]], University College London, UK<br />
* [[Elmer E|Elmér Eskil]], Lund University, SE <br />
* [[Hand SC|Hand Steven]], Louisiana State University, LA, US<br />
* [[Hickey AJ|Hickey Anthony J]], University of Auckland, NZ<br />
* [[Koopman WJ|Koopman Werner J]], Radboud Univ Nijmegen Medical Centre, NL<br />
* [[Tanaka M|Tanaka Masashi]], Toyko Metropolitan Institute of Gerontology, JP<br />
* [[Neufer PD|Neufer P Darrell]], East Carolina University, Greenville, NC, US<br />
* [[Ojuka E|Ojuka Edward]], University of Cape Town, South Africa<br />
* [[Pecina P|Pecina Petr]], Academy of Sciences of the Czech Republic, CZ<br />
* [[Perry CG|Perry Christopher G]], York University, Toronto, Canada<br />
* [[Rossignol R|Rossignol Rodrigue]], Université Victor Segalen-Bordeaux, FR<br />
* [[Vercesi AE|Vercesi Anibal E]], State University of Campinas, Sao Paulo, Brazil<br />
* [[Taiwan Society for Mitochondrial Research and Medicine|Wei Yau-Huei]], Mackay Medical College, New Taipei City, Taiwan<br />
* [[Zorzano A|Zorzano Antonio]], Universitat de Barcelona, Spain<br />
<br />
== Information for invited speakers==<br />
<br />
MiP2014 will be held at the University Centre Obergurgl, with maximum capacity of 124 persons and a price per person per night of € 65.- including a shared double room, breakfast, and dinner. MiP''conferences'' are organized as small meetings facilitating communication between senior and junior participants. In the tradition of MiP''conferences'', we are asking invited speakers to cooperate and try to receive local support for joining us at the prestigious Mitochondrial Physiology Conference (limited to 124 participants). Support will be provided to students and young investigators.<br />
<br />
== Post-conference O2k-Workshop ==<br />
<br />
A [http://www.oroboros.at/?IOC_2014-09_obergurgl post-conference workshop] is organized on the same venue, for hands-on training in mitochondrial respiratory physiology, introducing the combined measurement of high-resolution respirometry and fluorometry (OROBOROS INSTRUMENTS). Since participants come from all-over-the-world, including many students, the combination of the symposium with the workshop makes travelling more economical for the maximum of 24 workshop-participants. The post-conference workshop is financially completely separate from the symposium.<br />
<br />
[[File:MitoGlobal.jpg|left|40px|MitoGlobal]]<br />
== MiP''events'' ==<br />
are listed as '''[[MitoGlobal_Events]]'''.<br />
<br />
<br />
<br />
<br />
<br />
[[Category:MitoPedia]]<br />
[[Category:All]]</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MiP2014&diff=59359MiP20142014-05-05T08:06:56Z<p>Bauer Christiane: /* MiP2014 preliminary list of participants */</p>
<hr />
<div>[[Image:IUBMB-logo.gif|left|100px|link=http://www.iubmb.org|IUBMB - ''International Union of Biochemistry and Molecular Biology'']]<br />
[[Image:MiPsocietyLOGO.JPG|right|200px|link=http://www.mitophysiology.org|MiPsociety]]<br />
[http://www.mitophysiology.org/?MiP2014 '''<big>MiP2014 - 10<sup>th</sup> MiP''conference'': Joint IUBMB/MiP Symposium on Mitochondrial Physiology - a Point/Counterpoint Meeting</big>''']<br />
<br />
'''''International Union of Biochemistry and Molecular Biology''''' and '''Mitochondrial Physiology Society'''<br />
<br />
Obergurgl, Tyrol, Austria. 2014-Sep-08 to 12<br />
== MiP2014 - a point/counterpoint meeting ==<br />
<br />
Mitochondrial research has recently undergone a dramatic renaissance, due to numerous discoveries revealing its central role in cell death, disease pathology, aging, thermogenesis, oxidative stress, cell signalling, cellular regulation and cell bioenergetics. The aim of the small symposium is to bring together experts in mitochondrial research spanning from the biochemical and molecular level to the interactions within the cell. In addition to the latest scientific findings, new methodological developments in measuring mitochondrial function and dysfunction are presented. A point/counterpoint format is chosen to discuss selected hot topics and some fundamental methodological and conceptual issues.<br />
<br />
The expected attendance is max. 100 participants. ->> Compare [[MiP2013 Programme]]<br />
Abstracts and presentations: refer to [http://www.chem.qmul.ac.uk/iubmb/ IUBMB nomenclature]; >> [[MitoPedia Glossary: Respiratory states|MitoPedia Glossary: '''Respiratory states''']]<br />
<br />
== Programme structure ==<br />
<br />
In each of the 12 sessions a point/counterpoint introduction will be given by invited keynote speakers to provide a balanced overview and promote discussions. Short oral presentations are selected from abstracts, including student presentations. Rapid platform statements will introduce posters which contribute to a specific session. Posters are up throughout the meeting, and poster discussions are integrated into the platform sessions.<br />
<br />
=== A. Mitochondrial respiratory control === <br />
* A1. mtDNA, mt-phenotype and disease – is there biochemical and molecular-genetic evidence to explain observed associations? <br />
* A2. Fission and fusion, mitochondrial structure and function - is there a tight connection? <br />
* A3. Mitochondrial density and normalization of respiration – is there a general biochemical or molecular marker? <br />
* A4. Biochemical reserve capacity, respiratory control ratios and coupling efficiency – are conventional concepts inconsistent?<br />
<br />
=== B. Experimental protocols in mitochondrial biochemistry === <br />
* B1. Incubation media for mt-preparations and experimental temperature in functional studies of mammalian mitochondria – guided by concept or tradition? <br />
* B2. Intracellular oxygen pressure versus oxygen regimes in studies of ROS production and respiration - are mitochondria sources or sinks of ROS?<br />
* B3. Quantitative measurement of mt-membrane potential - potentiometric/fluorometric. <br />
* B4. Instrumental platforms in mitochondrial physiology – industrial presentations.<br />
<br />
=== C. Towards quality assurance === <br />
* C1. Do blood cells versus muscle biopsies provide valid models for diagnosis of mitochondrial pathologies? <br />
* C2. Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
* C3. From availability and quality control of chemicals to mitochondrial respiratory protocols – who controls? <br />
* C4. Standards for the diagnosis of mitochondrial pathologies – a challenge for collaboration with mitochondrial organizations?<br />
Particularly in view of Subtopic C, it may be attractive for mitochondrial organizations to send and support delegates. The conference will, therefore, provide a unique forum for international networking, and may inspire new national groups to be formed in contact with established mitochondrial organizations:<br />
>> '''[[MitoGlobal_Organizations]]'''<br />
<br />
== MiP2014 preliminary list of participants ==<br />
<br />
in progress<br />
<br />
{| class="wikitable"<br />
|-<br />
! Name !! Institution !! Preliminary title <br />
|-<br />
| [[Ali SS|Ali Sameh S]]||Zewail City for Science and Technology, ET||Oxygen-consuming and ROS-producing activities in synaptosomes.<br />
|-<br />
|-<br />
| [[Amoedo ND|Amoedo Nivea D.]]||Federal University of Rio de Janeiro, BR||Dissecting the role of mitocondria in metastasis.<br />
|-<br />
| [[Bauer CM|Bauer Christiane Maria]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Borutaite_V|Borutaite Vilmante]]||Lithuanian University of Health Sciences, Kaunas, LT||Controversies in testing integrity of mitochondrial membranes.<br />
|-<br />
| [[Brown D|Brown David]]||East Carolina University, NC, US||<br />
|-<br />
| [[Contreras-Ferrat A|Contreras-Ferrat Ariel]]||University of Chile, CL||<br />
|-<br />
| [[Coen P|Coen Paul]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||Does calorie restriction improve or worsen skeletal muscle mitochondrial performance?<br />
|-<br />
| [[Dohlmann T|Dohlmann Tine]]||University of Copenhagen, DK||<br />
|-<br />
| [[Eggimann S|Eggimann Sandra]]||University Hospital Berne University, Bern, CH||<br />
|-<br />
| [[Elmer E|Elmer Eskil]]||Wallenberg Neuroscience Center, Lund, SE||Ex vivo respiratory capacity of human blood cells - a SUITable application of the O2k Oxygraph / A novel pharmacological approach to counteract metabolic crisis.<br />
|-<br />
| [[Felding-Habermann B|Felding-Habermann Brunhilde]]||The Scripps Research Institute, La Jolla, CA, US||Mitochondrial functions in breast cancer progression.<br />
|-<br />
| [[Goodpaster BH|Goodpaster Bret]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Gnaiger E|Gnaiger Erich]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Grefte S|Grefte Sander]]||Radboud University Nijmegen Medical Centre, Nijmegen, NL||Pathophysiology of mitochondrial (dys)function in skeletal muscle.<br />
|-<br />
| [[Hahn D|Hahn Dagmar]]||Inselspital Bern, CH||Validation of oxygen consumption measurements in muscle and fibroblasts from patients with mitochondrial diseases. <br />
|-<br />
| [[Hand SC|Hand Steven C]]||Louisiana State University, LA, US||Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
|-<br />
| [[Harrison DK|Harrison David K]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Hayashi A|Hayashi Akira]]||DDU Oncology, Takeda Pharmaceutical Company Ltd, Kanagawa, JP||<br />
|-<br />
| [[Hepple_RT| Hepple Russel T.]]||McGill University, Montreal, CA||Mitochondrial involvement in aging muscle: cause and effect(or).<br />
|-<br />
| [[Herminghaus A|Herminghaus Anna]]||Uniklinikum Düsseldorf, DE||<br />
|-<br />
| [[Hey-Mogensen M|Hey-Mogensen Martin]]||Novo Nordisk A/S Diabetes NBEs & Obesity Biology, Måløv, DK||<br />
|-<br />
| [[Jana Prado F|Jana Prado Fabian]]||University of Chile, CL||<br />
|-<br />
| [[Kandashvili M|Kandashvili Manana ]]||Tallinn University, EE||Regulation of energy metabolism in human colorectal cancer cells ''in situ''.<br />
|-<br />
| [[Kane DA| Kane Daniel A.]]||St. Francis Xavier University, Antigonish, CA||Mitochondrial shuttles in muscle.<br />
|-<br />
| [[Kraunsoe R|Kraunsoe Regitze]]||University of Copenhagen, DK||<br />
|-<br />
| [[Koopman WJ|Koopman Werner J]]||Radboud University Nijmegen Medical Centre, NL||Redox regulation of mitochondrial dynamics.<br />
|-<br />
| [[Kowaltowski AJ|Kowaltowski Alicia J.]]||University of Sao Paulo, BR||<br />
|-<br />
| [[Laner V|Laner Verena]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Larsen S|Larsen Steen]]||University of Copenhagen, DK||Normalization of respiration.<br />
|-<br />
| [[Larsson NG|Larsson Nils-Göran]]||Max Planck Institute for the Biology of Ageing, Cologne, DE||Mouse models with mtDNA mutations.<br />
|-<br />
| [[Ojuka E|Ojuka Edward]]||University of Cape Town, ZA||Fission and fusion, mitochondrial structure and function - is there a tight connection?<br />
|-<br />
| [[Perry CG|Perry Christopher G]]||York University, Ontario, CA||Re-visiting critical experimental parameters to control when measuring respiratory kinetics in permeabilized muscle fibres.<br />
|-<br />
| [[Pesta D|Pesta Dominik]]||Yale University School of Medicine, CT, US||Dysregulation of muscle ATP synthesis in hypophosphatemic NaPi2a knockout mice and in a patient with hypophosphatemia due to a mutation in NaPi2c. <br />
|- <br />
| [[Picard_M|Picard Martin]]||University of Pennsylvania, PA, US||Quantitative regulation of nuclear gene expression by mitochondrial DNA<br />
heteroplasmy.<br />
|-<br />
| [[Pecina P|Pecina Petr]]||Academy of Sciences of the Czech Republic, Prague, CZ||The use of lymphocytes for diagnostics of mitochondrial disorders.<br />
|-<br />
| [[Purhonen J|Purhonen Janne]]||University Helsinki, FI||<br />
|-<br />
| [[Ristow M|Ristow Michael]]||ETH Zurich, CH||Promoting health and lifespan by increasing mitochondrial ROS formation.<br />
|-<br />
| [[Shirihai O|Shirihai Orian]]||The Shirihai Lab at Boston University, MA, US||<br />
|-<br />
| [[Skulachev VP|Skulachev Vladimir]]||Lomonosov Moscow State University, Moscow, RU||Mitochondria are sources of ROS rather than sinks: Physiological effects of mitochondria-targeted antioxidants.<br />
|-<br />
| [[Smith S|Smith Steven]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Sparks LM|Sparks Lauren M]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||The potential role of mitochondrial efficiency and energetics in the endurance-trained phenotype of NAMPT Transgenic (Tg) mice.<br />
|-<br />
| [[Starkov AA|Starkov Anatoly]]||Weill Medical College Cornell University, NY, US||<br />
|-<br />
| [[Stiban J|Stiban Johnny]]||Birzeit University, Ramallah, PS||Very long chain ceramides interfere with C16-ceramide-induced channel formation: A plausible mechanism for regulating the initiation of intrinsic apoptosis.<br />
|-<br />
| [[Zorzano A|Zorzano Antonio]]||Universitat de Barcelona, ES||Metabolic implications of mitofusin 2 dysfunction.<br />
|-<br />
| [[Sumbalova Z|Sumbalova Zuzana]]||Comenius University, Bratislava, SK||<br />
|}<br />
<br />
== Sponsors ==<br />
<br />
[[Image:OROBOROS Oxygraph-2k Logo.jpg|left|200px|link=http://www.oroboros.at|OROBOROS]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[Image:HAMLogoRGB.jpg|left|200px|link=http://www.hamilton.ch|Hamilton]]<br />
<br />
<br />
<br />
<br />
<br />
[[Image:Hansatech.jpg|left|200px|link=http://www.hansatech-instruments.com/|Hansatech]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
== Venue ==<br />
<br />
The symposium will be hosted in the '''University Center in Obergurgl''' 100 km from Innsbruck. The closest airport is in Innsbruck. Participants arriving at Munich airport will take the train or an airport shuttle to Innsbruck. We provide support in arranging the transfer between Munich and Innsbruck. A bus transfer is organized between Innsbruck and Obergurgl.<br />
<br />
The Obergurgl University Center is located at an altitude of 1940 meters above sea-level. The historical buildings, carefully refurbished and equipped with the latest technical systems, can accommodate around 70 persons in 43 bedrooms and includes conference rooms, the Alpine Research Center, laboratory, sports medicine, lounges and the restaurant. All rooms are equipped with an Internet connection. Additional participants are accommodated in smaller guest houses in 5 minutes walking distance. Meals are served for all participants in the University Center. Two conference rooms for a total of 200 persons and three small rooms for round table discussions are available. There is enough space for poster presentations and instrument exhibitions. The cosy bar and lounge area with an open fire place adds to the familiar atmosphere and supports the interactive spirit of the symposium.<br />
<br />
The small village of Obergurgl is situated in an impressive landscape at about 1900 m above sea level in the Ötz valley. It is surrounded by mountains, offering an exceptional view to the Ötz valley glaciers. During summer time the area is famous for hiking and for skiing in winter time. On the doorstep: one of Europe´s finest Biosphere Parks. The unique surroundings offer the possibility of combining scientific discussions while exploring the alpine environment during the famous “walks and talks”. The informal atmosphere of the workshop encourages also students and young post-docs to participate actively in scientific discussions and to develop future collaborations.<br />
<br />
University Centre Obergurgl, Gaisbergweg 5, 6456 Obergurgl, Tyrol, Austria.<br />
Tel.: +43 (0) 512 507 37201 | Fax: +43 (0) 512 507 37400 | Email: obergurgl@uibk.ac.at<br />
<br />
== International programme committee ==<br />
The international programme committee will provide scientific input by active participation, nominate further invited speakers to ensure balanced point/counterpoint introductions, and select the abstracts as a basis for participation (restricted number of participants), for oral 10+5 min presentations, and for flash presentations.<br />
<br />
Chair:<br />
* [[Gnaiger E|Gnaiger Erich]], Medical University Innsbruck, AT. - erich.gnaiger@mitophysiology.org<br />
Members:<br />
* [[Ali SS|Ali Sameh S]], Helmy Institute of Medical Sciences, Giza, Egypt<br />
* [[Borutaite V|Borutaite Vilma]], Lithuanian University of Health Sciences Kaunas, LT<br />
* [[Neurocon|Chakrabarti Sasanka]], Institute of Post-graduate Medical Education & Research, Kolkatta, India<br />
* [[Chinese Society of Mitochondrial Research and Medicine|Chen Quan]], Chinese Academy of Sciences, Nankai University, Beijing <br />
* [[Dela F|Dela Flemming]], University of Copenhagen, DK <br />
* [[Duchen MR|Duchen Michael R]], University College London, UK<br />
* [[Elmer E|Elmér Eskil]], Lund University, SE <br />
* [[Hand SC|Hand Steven]], Louisiana State University, LA, US<br />
* [[Hickey AJ|Hickey Anthony J]], University of Auckland, NZ<br />
* [[Koopman WJ|Koopman Werner J]], Radboud Univ Nijmegen Medical Centre, NL<br />
* [[Tanaka M|Tanaka Masashi]], Toyko Metropolitan Institute of Gerontology, JP<br />
* [[Neufer PD|Neufer P Darrell]], East Carolina University, Greenville, NC, US<br />
* [[Ojuka E|Ojuka Edward]], University of Cape Town, South Africa<br />
* [[Pecina P|Pecina Petr]], Academy of Sciences of the Czech Republic, CZ<br />
* [[Perry CG|Perry Christopher G]], York University, Toronto, Canada<br />
* [[Rossignol R|Rossignol Rodrigue]], Université Victor Segalen-Bordeaux, FR<br />
* [[Vercesi AE|Vercesi Anibal E]], State University of Campinas, Sao Paulo, Brazil<br />
* [[Taiwan Society for Mitochondrial Research and Medicine|Wei Yau-Huei]], Mackay Medical College, New Taipei City, Taiwan<br />
* [[Zorzano A|Zorzano Antonio]], Universitat de Barcelona, Spain<br />
<br />
== Information for invited speakers==<br />
<br />
MiP2014 will be held at the University Centre Obergurgl, with maximum capacity of 124 persons and a price per person per night of € 65.- including a shared double room, breakfast, and dinner. MiP''conferences'' are organized as small meetings facilitating communication between senior and junior participants. In the tradition of MiP''conferences'', we are asking invited speakers to cooperate and try to receive local support for joining us at the prestigious Mitochondrial Physiology Conference (limited to 124 participants). Support will be provided to students and young investigators.<br />
<br />
== Post-conference O2k-Workshop ==<br />
<br />
A [http://www.oroboros.at/?IOC_2014-09_obergurgl post-conference workshop] is organized on the same venue, for hands-on training in mitochondrial respiratory physiology, introducing the combined measurement of high-resolution respirometry and fluorometry (OROBOROS INSTRUMENTS). Since participants come from all-over-the-world, including many students, the combination of the symposium with the workshop makes travelling more economical for the maximum of 24 workshop-participants. The post-conference workshop is financially completely separate from the symposium.<br />
<br />
[[File:MitoGlobal.jpg|left|40px|MitoGlobal]]<br />
== MiP''events'' ==<br />
are listed as '''[[MitoGlobal_Events]]'''.<br />
<br />
<br />
<br />
<br />
<br />
[[Category:MitoPedia]]<br />
[[Category:All]]</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MiP2014&diff=59358MiP20142014-05-05T08:05:21Z<p>Bauer Christiane: /* MiP2014 preliminary list of participants */</p>
<hr />
<div>[[Image:IUBMB-logo.gif|left|100px|link=http://www.iubmb.org|IUBMB - ''International Union of Biochemistry and Molecular Biology'']]<br />
[[Image:MiPsocietyLOGO.JPG|right|200px|link=http://www.mitophysiology.org|MiPsociety]]<br />
[http://www.mitophysiology.org/?MiP2014 '''<big>MiP2014 - 10<sup>th</sup> MiP''conference'': Joint IUBMB/MiP Symposium on Mitochondrial Physiology - a Point/Counterpoint Meeting</big>''']<br />
<br />
'''''International Union of Biochemistry and Molecular Biology''''' and '''Mitochondrial Physiology Society'''<br />
<br />
Obergurgl, Tyrol, Austria. 2014-Sep-08 to 12<br />
== MiP2014 - a point/counterpoint meeting ==<br />
<br />
Mitochondrial research has recently undergone a dramatic renaissance, due to numerous discoveries revealing its central role in cell death, disease pathology, aging, thermogenesis, oxidative stress, cell signalling, cellular regulation and cell bioenergetics. The aim of the small symposium is to bring together experts in mitochondrial research spanning from the biochemical and molecular level to the interactions within the cell. In addition to the latest scientific findings, new methodological developments in measuring mitochondrial function and dysfunction are presented. A point/counterpoint format is chosen to discuss selected hot topics and some fundamental methodological and conceptual issues.<br />
<br />
The expected attendance is max. 100 participants. ->> Compare [[MiP2013 Programme]]<br />
Abstracts and presentations: refer to [http://www.chem.qmul.ac.uk/iubmb/ IUBMB nomenclature]; >> [[MitoPedia Glossary: Respiratory states|MitoPedia Glossary: '''Respiratory states''']]<br />
<br />
== Programme structure ==<br />
<br />
In each of the 12 sessions a point/counterpoint introduction will be given by invited keynote speakers to provide a balanced overview and promote discussions. Short oral presentations are selected from abstracts, including student presentations. Rapid platform statements will introduce posters which contribute to a specific session. Posters are up throughout the meeting, and poster discussions are integrated into the platform sessions.<br />
<br />
=== A. Mitochondrial respiratory control === <br />
* A1. mtDNA, mt-phenotype and disease – is there biochemical and molecular-genetic evidence to explain observed associations? <br />
* A2. Fission and fusion, mitochondrial structure and function - is there a tight connection? <br />
* A3. Mitochondrial density and normalization of respiration – is there a general biochemical or molecular marker? <br />
* A4. Biochemical reserve capacity, respiratory control ratios and coupling efficiency – are conventional concepts inconsistent?<br />
<br />
=== B. Experimental protocols in mitochondrial biochemistry === <br />
* B1. Incubation media for mt-preparations and experimental temperature in functional studies of mammalian mitochondria – guided by concept or tradition? <br />
* B2. Intracellular oxygen pressure versus oxygen regimes in studies of ROS production and respiration - are mitochondria sources or sinks of ROS?<br />
* B3. Quantitative measurement of mt-membrane potential - potentiometric/fluorometric. <br />
* B4. Instrumental platforms in mitochondrial physiology – industrial presentations.<br />
<br />
=== C. Towards quality assurance === <br />
* C1. Do blood cells versus muscle biopsies provide valid models for diagnosis of mitochondrial pathologies? <br />
* C2. Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
* C3. From availability and quality control of chemicals to mitochondrial respiratory protocols – who controls? <br />
* C4. Standards for the diagnosis of mitochondrial pathologies – a challenge for collaboration with mitochondrial organizations?<br />
Particularly in view of Subtopic C, it may be attractive for mitochondrial organizations to send and support delegates. The conference will, therefore, provide a unique forum for international networking, and may inspire new national groups to be formed in contact with established mitochondrial organizations:<br />
>> '''[[MitoGlobal_Organizations]]'''<br />
<br />
== MiP2014 preliminary list of participants ==<br />
<br />
in progress<br />
<br />
{| class="wikitable"<br />
|-<br />
! Name !! Institution !! Preliminary title <br />
|-<br />
| [[Ali SS|Ali Sameh S]]||Zewail City for Science and Technology, ET||Oxygen-consuming and ROS-producing activities in synaptosomes.<br />
|-<br />
|-<br />
| [[Amoedo ND|Amoedo Nivea D.]]||Federal University of Rio de Janeiro, BR||Dissecting the role of mitocondria in metastasis.<br />
|-<br />
| [[Bauer CM|Bauer Christiane Maria]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Borutaite_V|Borutaite Vilmante]]||Lithuanian University of Health Sciences, Kaunas, LT||Controversies in testing integrity of mitochondrial membranes.<br />
|-<br />
| [[Brown D|Brown David]]||East Carolina University, NC, US||<br />
|-<br />
| [[Contreras-Ferrat A|Contreras-Ferrat Ariel]]||University of Chile, CL||<br />
|-<br />
| [[Coen P|Coen Paul]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||Does calorie restriction improve or worsen skeletal muscle mitochondrial performance?<br />
|-<br />
| [[Dohlmann T|Dohlmann Tine]]||University of Copenhagen, DK||<br />
|-<br />
| [[Eggimann S|Eggimann Sandra]]||University Hospital Berne University||<br />
|-<br />
| [[Elmer E|Elmer Eskil]]||Wallenberg Neuroscience Center, Lund, SE||Ex vivo respiratory capacity of human blood cells - a SUITable application of the O2k Oxygraph / A novel pharmacological approach to counteract metabolic crisis.<br />
|-<br />
| [[Felding-Habermann B|Felding-Habermann Brunhilde]]||The Scripps Research Institute, La Jolla, CA, US||Mitochondrial functions in breast cancer progression.<br />
|-<br />
| [[Goodpaster BH|Goodpaster Bret]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Gnaiger E|Gnaiger Erich]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Grefte S|Grefte Sander]]||Radboud University Nijmegen Medical Centre, Nijmegen, NL||Pathophysiology of mitochondrial (dys)function in skeletal muscle.<br />
|-<br />
| [[Hahn D|Hahn Dagmar]]||Inselspital Bern, CH||Validation of oxygen consumption measurements in muscle and fibroblasts from patients with mitochondrial diseases. <br />
|-<br />
| [[Hand SC|Hand Steven C]]||Louisiana State University, LA, US||Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
|-<br />
| [[Harrison DK|Harrison David K]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Hayashi A|Hayashi Akira]]||DDU Oncology, Takeda Pharmaceutical Company Ltd, Kanagawa, JP||<br />
|-<br />
| [[Hepple_RT| Hepple Russel T.]]||McGill University, Montreal, CA||Mitochondrial involvement in aging muscle: cause and effect(or).<br />
|-<br />
| [[Herminghaus A|Herminghaus Anna]]||Uniklinikum Düsseldorf, DE||<br />
|-<br />
| [[Hey-Mogensen M|Hey-Mogensen Martin]]||Novo Nordisk A/S Diabetes NBEs & Obesity Biology, Måløv, DK||<br />
|-<br />
| [[Jana Prado F|Jana Prado Fabian]]||University of Chile, CL||<br />
|-<br />
| [[Kandashvili M|Kandashvili Manana ]]||Tallinn University, EE||Regulation of energy metabolism in human colorectal cancer cells ''in situ''.<br />
|-<br />
| [[Kane DA| Kane Daniel A.]]||St. Francis Xavier University, Antigonish, CA||Mitochondrial shuttles in muscle.<br />
|-<br />
| [[Kraunsoe R|Kraunsoe Regitze]]||University of Copenhagen, DK||<br />
|-<br />
| [[Koopman WJ|Koopman Werner J]]||Radboud University Nijmegen Medical Centre, NL||Redox regulation of mitochondrial dynamics.<br />
|-<br />
| [[Kowaltowski AJ|Kowaltowski Alicia J.]]||University of Sao Paulo, BR||<br />
|-<br />
| [[Laner V|Laner Verena]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Larsen S|Larsen Steen]]||University of Copenhagen, DK||Normalization of respiration.<br />
|-<br />
| [[Larsson NG|Larsson Nils-Göran]]||Max Planck Institute for the Biology of Ageing, Cologne, DE||Mouse models with mtDNA mutations.<br />
|-<br />
| [[Ojuka E|Ojuka Edward]]||University of Cape Town, ZA||Fission and fusion, mitochondrial structure and function - is there a tight connection?<br />
|-<br />
| [[Perry CG|Perry Christopher G]]||York University, Ontario, CA||Re-visiting critical experimental parameters to control when measuring respiratory kinetics in permeabilized muscle fibres.<br />
|-<br />
| [[Pesta D|Pesta Dominik]]||Yale University School of Medicine, CT, US||Dysregulation of muscle ATP synthesis in hypophosphatemic NaPi2a knockout mice and in a patient with hypophosphatemia due to a mutation in NaPi2c. <br />
|- <br />
| [[Picard_M|Picard Martin]]||University of Pennsylvania, PA, US||Quantitative regulation of nuclear gene expression by mitochondrial DNA<br />
heteroplasmy.<br />
|-<br />
| [[Pecina P|Pecina Petr]]||Academy of Sciences of the Czech Republic, Prague, CZ||The use of lymphocytes for diagnostics of mitochondrial disorders.<br />
|-<br />
| [[Purhonen J|Purhonen Janne]]||University Helsinki, FI||<br />
|-<br />
| [[Ristow M|Ristow Michael]]||ETH Zurich, CH||Promoting health and lifespan by increasing mitochondrial ROS formation.<br />
|-<br />
| [[Shirihai O|Shirihai Orian]]||The Shirihai Lab at Boston University, MA, US||<br />
|-<br />
| [[Skulachev VP|Skulachev Vladimir]]||Lomonosov Moscow State University, Moscow, RU||Mitochondria are sources of ROS rather than sinks: Physiological effects of mitochondria-targeted antioxidants.<br />
|-<br />
| [[Smith S|Smith Steven]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Sparks LM|Sparks Lauren M]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||The potential role of mitochondrial efficiency and energetics in the endurance-trained phenotype of NAMPT Transgenic (Tg) mice.<br />
|-<br />
| [[Starkov AA|Starkov Anatoly]]||Weill Medical College Cornell University, NY, US||<br />
|-<br />
| [[Stiban J|Stiban Johnny]]||Birzeit University, Ramallah, PS||Very long chain ceramides interfere with C16-ceramide-induced channel formation: A plausible mechanism for regulating the initiation of intrinsic apoptosis.<br />
|-<br />
| [[Zorzano A|Zorzano Antonio]]||Universitat de Barcelona, ES||Metabolic implications of mitofusin 2 dysfunction.<br />
|-<br />
| [[Sumbalova Z|Sumbalova Zuzana]]||Comenius University, Bratislava, SK||<br />
|}<br />
<br />
== Sponsors ==<br />
<br />
[[Image:OROBOROS Oxygraph-2k Logo.jpg|left|200px|link=http://www.oroboros.at|OROBOROS]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[Image:HAMLogoRGB.jpg|left|200px|link=http://www.hamilton.ch|Hamilton]]<br />
<br />
<br />
<br />
<br />
<br />
[[Image:Hansatech.jpg|left|200px|link=http://www.hansatech-instruments.com/|Hansatech]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
== Venue ==<br />
<br />
The symposium will be hosted in the '''University Center in Obergurgl''' 100 km from Innsbruck. The closest airport is in Innsbruck. Participants arriving at Munich airport will take the train or an airport shuttle to Innsbruck. We provide support in arranging the transfer between Munich and Innsbruck. A bus transfer is organized between Innsbruck and Obergurgl.<br />
<br />
The Obergurgl University Center is located at an altitude of 1940 meters above sea-level. The historical buildings, carefully refurbished and equipped with the latest technical systems, can accommodate around 70 persons in 43 bedrooms and includes conference rooms, the Alpine Research Center, laboratory, sports medicine, lounges and the restaurant. All rooms are equipped with an Internet connection. Additional participants are accommodated in smaller guest houses in 5 minutes walking distance. Meals are served for all participants in the University Center. Two conference rooms for a total of 200 persons and three small rooms for round table discussions are available. There is enough space for poster presentations and instrument exhibitions. The cosy bar and lounge area with an open fire place adds to the familiar atmosphere and supports the interactive spirit of the symposium.<br />
<br />
The small village of Obergurgl is situated in an impressive landscape at about 1900 m above sea level in the Ötz valley. It is surrounded by mountains, offering an exceptional view to the Ötz valley glaciers. During summer time the area is famous for hiking and for skiing in winter time. On the doorstep: one of Europe´s finest Biosphere Parks. The unique surroundings offer the possibility of combining scientific discussions while exploring the alpine environment during the famous “walks and talks”. The informal atmosphere of the workshop encourages also students and young post-docs to participate actively in scientific discussions and to develop future collaborations.<br />
<br />
University Centre Obergurgl, Gaisbergweg 5, 6456 Obergurgl, Tyrol, Austria.<br />
Tel.: +43 (0) 512 507 37201 | Fax: +43 (0) 512 507 37400 | Email: obergurgl@uibk.ac.at<br />
<br />
== International programme committee ==<br />
The international programme committee will provide scientific input by active participation, nominate further invited speakers to ensure balanced point/counterpoint introductions, and select the abstracts as a basis for participation (restricted number of participants), for oral 10+5 min presentations, and for flash presentations.<br />
<br />
Chair:<br />
* [[Gnaiger E|Gnaiger Erich]], Medical University Innsbruck, AT. - erich.gnaiger@mitophysiology.org<br />
Members:<br />
* [[Ali SS|Ali Sameh S]], Helmy Institute of Medical Sciences, Giza, Egypt<br />
* [[Borutaite V|Borutaite Vilma]], Lithuanian University of Health Sciences Kaunas, LT<br />
* [[Neurocon|Chakrabarti Sasanka]], Institute of Post-graduate Medical Education & Research, Kolkatta, India<br />
* [[Chinese Society of Mitochondrial Research and Medicine|Chen Quan]], Chinese Academy of Sciences, Nankai University, Beijing <br />
* [[Dela F|Dela Flemming]], University of Copenhagen, DK <br />
* [[Duchen MR|Duchen Michael R]], University College London, UK<br />
* [[Elmer E|Elmér Eskil]], Lund University, SE <br />
* [[Hand SC|Hand Steven]], Louisiana State University, LA, US<br />
* [[Hickey AJ|Hickey Anthony J]], University of Auckland, NZ<br />
* [[Koopman WJ|Koopman Werner J]], Radboud Univ Nijmegen Medical Centre, NL<br />
* [[Tanaka M|Tanaka Masashi]], Toyko Metropolitan Institute of Gerontology, JP<br />
* [[Neufer PD|Neufer P Darrell]], East Carolina University, Greenville, NC, US<br />
* [[Ojuka E|Ojuka Edward]], University of Cape Town, South Africa<br />
* [[Pecina P|Pecina Petr]], Academy of Sciences of the Czech Republic, CZ<br />
* [[Perry CG|Perry Christopher G]], York University, Toronto, Canada<br />
* [[Rossignol R|Rossignol Rodrigue]], Université Victor Segalen-Bordeaux, FR<br />
* [[Vercesi AE|Vercesi Anibal E]], State University of Campinas, Sao Paulo, Brazil<br />
* [[Taiwan Society for Mitochondrial Research and Medicine|Wei Yau-Huei]], Mackay Medical College, New Taipei City, Taiwan<br />
* [[Zorzano A|Zorzano Antonio]], Universitat de Barcelona, Spain<br />
<br />
== Information for invited speakers==<br />
<br />
MiP2014 will be held at the University Centre Obergurgl, with maximum capacity of 124 persons and a price per person per night of € 65.- including a shared double room, breakfast, and dinner. MiP''conferences'' are organized as small meetings facilitating communication between senior and junior participants. In the tradition of MiP''conferences'', we are asking invited speakers to cooperate and try to receive local support for joining us at the prestigious Mitochondrial Physiology Conference (limited to 124 participants). Support will be provided to students and young investigators.<br />
<br />
== Post-conference O2k-Workshop ==<br />
<br />
A [http://www.oroboros.at/?IOC_2014-09_obergurgl post-conference workshop] is organized on the same venue, for hands-on training in mitochondrial respiratory physiology, introducing the combined measurement of high-resolution respirometry and fluorometry (OROBOROS INSTRUMENTS). Since participants come from all-over-the-world, including many students, the combination of the symposium with the workshop makes travelling more economical for the maximum of 24 workshop-participants. The post-conference workshop is financially completely separate from the symposium.<br />
<br />
[[File:MitoGlobal.jpg|left|40px|MitoGlobal]]<br />
== MiP''events'' ==<br />
are listed as '''[[MitoGlobal_Events]]'''.<br />
<br />
<br />
<br />
<br />
<br />
[[Category:MitoPedia]]<br />
[[Category:All]]</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MiP2014&diff=59356MiP20142014-05-05T07:32:59Z<p>Bauer Christiane: /* MiP2014 preliminary list of participants */</p>
<hr />
<div>[[Image:IUBMB-logo.gif|left|100px|link=http://www.iubmb.org|IUBMB - ''International Union of Biochemistry and Molecular Biology'']]<br />
[[Image:MiPsocietyLOGO.JPG|right|200px|link=http://www.mitophysiology.org|MiPsociety]]<br />
[http://www.mitophysiology.org/?MiP2014 '''<big>MiP2014 - 10<sup>th</sup> MiP''conference'': Joint IUBMB/MiP Symposium on Mitochondrial Physiology - a Point/Counterpoint Meeting</big>''']<br />
<br />
'''''International Union of Biochemistry and Molecular Biology''''' and '''Mitochondrial Physiology Society'''<br />
<br />
Obergurgl, Tyrol, Austria. 2014-Sep-08 to 12<br />
== MiP2014 - a point/counterpoint meeting ==<br />
<br />
Mitochondrial research has recently undergone a dramatic renaissance, due to numerous discoveries revealing its central role in cell death, disease pathology, aging, thermogenesis, oxidative stress, cell signalling, cellular regulation and cell bioenergetics. The aim of the small symposium is to bring together experts in mitochondrial research spanning from the biochemical and molecular level to the interactions within the cell. In addition to the latest scientific findings, new methodological developments in measuring mitochondrial function and dysfunction are presented. A point/counterpoint format is chosen to discuss selected hot topics and some fundamental methodological and conceptual issues.<br />
<br />
The expected attendance is max. 100 participants. ->> Compare [[MiP2013 Programme]]<br />
Abstracts and presentations: refer to [http://www.chem.qmul.ac.uk/iubmb/ IUBMB nomenclature]; >> [[MitoPedia Glossary: Respiratory states|MitoPedia Glossary: '''Respiratory states''']]<br />
<br />
== Programme structure ==<br />
<br />
In each of the 12 sessions a point/counterpoint introduction will be given by invited keynote speakers to provide a balanced overview and promote discussions. Short oral presentations are selected from abstracts, including student presentations. Rapid platform statements will introduce posters which contribute to a specific session. Posters are up throughout the meeting, and poster discussions are integrated into the platform sessions.<br />
<br />
=== A. Mitochondrial respiratory control === <br />
* A1. mtDNA, mt-phenotype and disease – is there biochemical and molecular-genetic evidence to explain observed associations? <br />
* A2. Fission and fusion, mitochondrial structure and function - is there a tight connection? <br />
* A3. Mitochondrial density and normalization of respiration – is there a general biochemical or molecular marker? <br />
* A4. Biochemical reserve capacity, respiratory control ratios and coupling efficiency – are conventional concepts inconsistent?<br />
<br />
=== B. Experimental protocols in mitochondrial biochemistry === <br />
* B1. Incubation media for mt-preparations and experimental temperature in functional studies of mammalian mitochondria – guided by concept or tradition? <br />
* B2. Intracellular oxygen pressure versus oxygen regimes in studies of ROS production and respiration - are mitochondria sources or sinks of ROS?<br />
* B3. Quantitative measurement of mt-membrane potential - potentiometric/fluorometric. <br />
* B4. Instrumental platforms in mitochondrial physiology – industrial presentations.<br />
<br />
=== C. Towards quality assurance === <br />
* C1. Do blood cells versus muscle biopsies provide valid models for diagnosis of mitochondrial pathologies? <br />
* C2. Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
* C3. From availability and quality control of chemicals to mitochondrial respiratory protocols – who controls? <br />
* C4. Standards for the diagnosis of mitochondrial pathologies – a challenge for collaboration with mitochondrial organizations?<br />
Particularly in view of Subtopic C, it may be attractive for mitochondrial organizations to send and support delegates. The conference will, therefore, provide a unique forum for international networking, and may inspire new national groups to be formed in contact with established mitochondrial organizations:<br />
>> '''[[MitoGlobal_Organizations]]'''<br />
<br />
== MiP2014 preliminary list of participants ==<br />
<br />
in progress<br />
<br />
{| class="wikitable"<br />
|-<br />
! Name !! Institution !! Preliminary title <br />
|-<br />
| [[Ali SS|Ali Sameh S]]||Zewail City for Science and Technology, ET||Oxygen-consuming and ROS-producing activities in synaptosomes.<br />
|-<br />
|-<br />
| [[Amoedo ND|Amoedo Nivea D.]]||Federal University of Rio de Janeiro, BR||Dissecting the role of mitocondria in metastasis.<br />
|-<br />
| [[Bauer CM|Bauer Christiane Maria]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Borutaite_V|Borutaite Vilmante]]||Lithuanian University of Health Sciences, Kaunas, LT||Controversies in testing integrity of mitochondrial membranes.<br />
|-<br />
| [[Brown D|Brown David]]||East Carolina University, NC, US||<br />
|-<br />
| [[Contreras-Ferrat A|Contreras-Ferrat Ariel]]||University of Chile, CL||<br />
|-<br />
| [[Coen P|Coen Paul]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||Does calorie restriction improve or worsen skeletal muscle mitochondrial performance?<br />
|-<br />
| [[Dohlmann T|Dohlmann Tine]]||University of Copenhagen, DK||<br />
|-<br />
| [[Eggimann S|Eggimann Sandra]]||University Hospital Berne University||<br />
|-<br />
| [[Elmer E|Elmer Eskil]]||Wallenberg Neuroscience Center, Lund, SE||Ex vivo respiratory capacity of human blood cells - a SUITable application of the O2k Oxygraph / A novel pharmacological approach to counteract metabolic crisis.<br />
|-<br />
| [[Felding-Habermann B|Felding-Habermann Brunhilde]]||The Scripps Research Institute, La Jolla, CA, US||Mitochondrial functions in breast cancer progression.<br />
|-<br />
| [[Goodpaster BH|Goodpaster Bret]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Gnaiger E|Gnaiger Erich]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Grefte S|Grefte Sander]]||Radboud University Nijmegen Medical Centre, Nijmegen, NL||Pathophysiology of mitochondrial (dys)function in skeletal muscle.<br />
|-<br />
| [[Hahn D|Hahn Dagmar]]||Inselspital Bern, CH||Validation of oxygen consumption measurements in muscle and fibroblasts from patients with mitochondrial diseases. <br />
|-<br />
| [[Hand SC|Hand Steven C]]||Louisiana State University, LA, US||Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
|-<br />
| [[Harrison DK|Harrison David K]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Hayashi A|Hayashi Akira]]||DDU Oncology, Takeda Pharmaceutical Company Ltd, Kanagawa, JP||<br />
|-<br />
| [[Hepple_RT| Hepple Russel T.]]||McGill University, Montreal, CA||Mitochondrial involvement in aging muscle: cause and effect(or).<br />
|-<br />
| [[Hey-Mogensen M|Hey-Mogensen Martin]]||Novo Nordisk A/S Diabetes NBEs & Obesity Biology, Måløv, DK||<br />
|-<br />
| [[Jana Prado F|Jana Prado Fabian]]||University of Chile, CL||<br />
|-<br />
| [[Kandashvili M|Kandashvili Manana ]]||Tallinn University, EE||Regulation of energy metabolism in human colorectal cancer cells ''in situ''.<br />
|-<br />
| [[Kane DA| Kane Daniel A.]]||St. Francis Xavier University, Antigonish, CA||Mitochondrial shuttles in muscle.<br />
|-<br />
| [[Kraunsoe R|Kraunsoe Regitze]]||University of Copenhagen, DK||<br />
|-<br />
| [[Koopman WJ|Koopman Werner J]]||Radboud University Nijmegen Medical Centre, NL||Redox regulation of mitochondrial dynamics.<br />
|-<br />
| [[Kowaltowski AJ|Kowaltowski Alicia J.]]||University of Sao Paulo, BR||<br />
|-<br />
| [[Laner V|Laner Verena]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Larsen S|Larsen Steen]]||University of Copenhagen, DK||Normalization of respiration.<br />
|-<br />
| [[Larsson NG|Larsson Nils-Göran]]||Max Planck Institute for the Biology of Ageing, Cologne, DE||Mouse models with mtDNA mutations.<br />
|-<br />
| [[Ojuka E|Ojuka Edward]]||University of Cape Town, ZA||Fission and fusion, mitochondrial structure and function - is there a tight connection?<br />
|-<br />
| [[Perry CG|Perry Christopher G]]||York University, Ontario, CA||Re-visiting critical experimental parameters to control when measuring respiratory kinetics in permeabilized muscle fibres.<br />
|-<br />
| [[Pesta D|Pesta Dominik]]||Yale University School of Medicine, CT, US||Dysregulation of muscle ATP synthesis in hypophosphatemic NaPi2a knockout mice and in a patient with hypophosphatemia due to a mutation in NaPi2c. <br />
|- <br />
| [[Picard_M|Picard Martin]]||University of Pennsylvania, PA, US||Quantitative regulation of nuclear gene expression by mitochondrial DNA<br />
heteroplasmy.<br />
|-<br />
| [[Pecina P|Pecina Petr]]||Academy of Sciences of the Czech Republic, Prague, CZ||The use of lymphocytes for diagnostics of mitochondrial disorders.<br />
|-<br />
| [[Purhonen J|Purhonen Janne]]||University Helsinki, FI||<br />
|-<br />
| [[Ristow M|Ristow Michael]]||ETH Zurich, CH||Promoting health and lifespan by increasing mitochondrial ROS formation.<br />
|-<br />
| [[Shirihai O|Shirihai Orian]]||The Shirihai Lab at Boston University, MA, US||<br />
|-<br />
| [[Skulachev VP|Skulachev Vladimir]]||Lomonosov Moscow State University, Moscow, RU||Mitochondria are sources of ROS rather than sinks: Physiological effects of mitochondria-targeted antioxidants.<br />
|-<br />
| [[Smith S|Smith Steven]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Sparks LM|Sparks Lauren M]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||The potential role of mitochondrial efficiency and energetics in the endurance-trained phenotype of NAMPT Transgenic (Tg) mice.<br />
|-<br />
| [[Starkov AA|Starkov Anatoly]]||Weill Medical College Cornell University, NY, US||<br />
|-<br />
| [[Stiban J|Stiban Johnny]]||Birzeit University, Ramallah, PS||Very long chain ceramides interfere with C16-ceramide-induced channel formation: A plausible mechanism for regulating the initiation of intrinsic apoptosis.<br />
|-<br />
| [[Zorzano A|Zorzano Antonio]]||Universitat de Barcelona, ES||Metabolic implications of mitofusin 2 dysfunction.<br />
|-<br />
| [[Sumbalova Z|Sumbalova Zuzana]]||Comenius University, Bratislava, SK||<br />
|}<br />
<br />
== Sponsors ==<br />
<br />
[[Image:OROBOROS Oxygraph-2k Logo.jpg|left|200px|link=http://www.oroboros.at|OROBOROS]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[Image:HAMLogoRGB.jpg|left|200px|link=http://www.hamilton.ch|Hamilton]]<br />
<br />
<br />
<br />
<br />
<br />
[[Image:Hansatech.jpg|left|200px|link=http://www.hansatech-instruments.com/|Hansatech]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
== Venue ==<br />
<br />
The symposium will be hosted in the '''University Center in Obergurgl''' 100 km from Innsbruck. The closest airport is in Innsbruck. Participants arriving at Munich airport will take the train or an airport shuttle to Innsbruck. We provide support in arranging the transfer between Munich and Innsbruck. A bus transfer is organized between Innsbruck and Obergurgl.<br />
<br />
The Obergurgl University Center is located at an altitude of 1940 meters above sea-level. The historical buildings, carefully refurbished and equipped with the latest technical systems, can accommodate around 70 persons in 43 bedrooms and includes conference rooms, the Alpine Research Center, laboratory, sports medicine, lounges and the restaurant. All rooms are equipped with an Internet connection. Additional participants are accommodated in smaller guest houses in 5 minutes walking distance. Meals are served for all participants in the University Center. Two conference rooms for a total of 200 persons and three small rooms for round table discussions are available. There is enough space for poster presentations and instrument exhibitions. The cosy bar and lounge area with an open fire place adds to the familiar atmosphere and supports the interactive spirit of the symposium.<br />
<br />
The small village of Obergurgl is situated in an impressive landscape at about 1900 m above sea level in the Ötz valley. It is surrounded by mountains, offering an exceptional view to the Ötz valley glaciers. During summer time the area is famous for hiking and for skiing in winter time. On the doorstep: one of Europe´s finest Biosphere Parks. The unique surroundings offer the possibility of combining scientific discussions while exploring the alpine environment during the famous “walks and talks”. The informal atmosphere of the workshop encourages also students and young post-docs to participate actively in scientific discussions and to develop future collaborations.<br />
<br />
University Centre Obergurgl, Gaisbergweg 5, 6456 Obergurgl, Tyrol, Austria.<br />
Tel.: +43 (0) 512 507 37201 | Fax: +43 (0) 512 507 37400 | Email: obergurgl@uibk.ac.at<br />
<br />
== International programme committee ==<br />
The international programme committee will provide scientific input by active participation, nominate further invited speakers to ensure balanced point/counterpoint introductions, and select the abstracts as a basis for participation (restricted number of participants), for oral 10+5 min presentations, and for flash presentations.<br />
<br />
Chair:<br />
* [[Gnaiger E|Gnaiger Erich]], Medical University Innsbruck, AT. - erich.gnaiger@mitophysiology.org<br />
Members:<br />
* [[Ali SS|Ali Sameh S]], Helmy Institute of Medical Sciences, Giza, Egypt<br />
* [[Borutaite V|Borutaite Vilma]], Lithuanian University of Health Sciences Kaunas, LT<br />
* [[Neurocon|Chakrabarti Sasanka]], Institute of Post-graduate Medical Education & Research, Kolkatta, India<br />
* [[Chinese Society of Mitochondrial Research and Medicine|Chen Quan]], Chinese Academy of Sciences, Nankai University, Beijing <br />
* [[Dela F|Dela Flemming]], University of Copenhagen, DK <br />
* [[Duchen MR|Duchen Michael R]], University College London, UK<br />
* [[Elmer E|Elmér Eskil]], Lund University, SE <br />
* [[Hand SC|Hand Steven]], Louisiana State University, LA, US<br />
* [[Hickey AJ|Hickey Anthony J]], University of Auckland, NZ<br />
* [[Koopman WJ|Koopman Werner J]], Radboud Univ Nijmegen Medical Centre, NL<br />
* [[Tanaka M|Tanaka Masashi]], Toyko Metropolitan Institute of Gerontology, JP<br />
* [[Neufer PD|Neufer P Darrell]], East Carolina University, Greenville, NC, US<br />
* [[Ojuka E|Ojuka Edward]], University of Cape Town, South Africa<br />
* [[Pecina P|Pecina Petr]], Academy of Sciences of the Czech Republic, CZ<br />
* [[Perry CG|Perry Christopher G]], York University, Toronto, Canada<br />
* [[Rossignol R|Rossignol Rodrigue]], Université Victor Segalen-Bordeaux, FR<br />
* [[Vercesi AE|Vercesi Anibal E]], State University of Campinas, Sao Paulo, Brazil<br />
* [[Taiwan Society for Mitochondrial Research and Medicine|Wei Yau-Huei]], Mackay Medical College, New Taipei City, Taiwan<br />
* [[Zorzano A|Zorzano Antonio]], Universitat de Barcelona, Spain<br />
<br />
== Information for invited speakers==<br />
<br />
MiP2014 will be held at the University Centre Obergurgl, with maximum capacity of 124 persons and a price per person per night of € 65.- including a shared double room, breakfast, and dinner. MiP''conferences'' are organized as small meetings facilitating communication between senior and junior participants. In the tradition of MiP''conferences'', we are asking invited speakers to cooperate and try to receive local support for joining us at the prestigious Mitochondrial Physiology Conference (limited to 124 participants). Support will be provided to students and young investigators.<br />
<br />
== Post-conference O2k-Workshop ==<br />
<br />
A [http://www.oroboros.at/?IOC_2014-09_obergurgl post-conference workshop] is organized on the same venue, for hands-on training in mitochondrial respiratory physiology, introducing the combined measurement of high-resolution respirometry and fluorometry (OROBOROS INSTRUMENTS). Since participants come from all-over-the-world, including many students, the combination of the symposium with the workshop makes travelling more economical for the maximum of 24 workshop-participants. The post-conference workshop is financially completely separate from the symposium.<br />
<br />
[[File:MitoGlobal.jpg|left|40px|MitoGlobal]]<br />
== MiP''events'' ==<br />
are listed as '''[[MitoGlobal_Events]]'''.<br />
<br />
<br />
<br />
<br />
<br />
[[Category:MitoPedia]]<br />
[[Category:All]]</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=Eggimann_S&diff=59355Eggimann S2014-05-05T07:31:37Z<p>Bauer Christiane: Created page with "{{Person |lastname=Eggimann |firstname=Sandra |title= |institution=University Hospital Berne University Institute of Clinical Chemistry |address=Inselspital INO F 511/UKC |area ..."</p>
<hr />
<div>{{Person<br />
|lastname=Eggimann<br />
|firstname=Sandra<br />
|title=<br />
|institution=University Hospital Berne University<br />
<br />
Institute of Clinical Chemistry<br />
|address=Inselspital INO F 511/UKC<br />
|area code=3010<br />
|city=Bern<br />
|country=Switzerland<br />
|mailaddress=sandra.eggimann@insel.ch<br />
}}<br />
{{Labelingperson}}<br />
==Participated at==</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MiP2014&diff=59194MiP20142014-04-29T11:59:32Z<p>Bauer Christiane: /* MiP2014 preliminary list of participants */</p>
<hr />
<div>[[Image:IUBMB-logo.gif|left|100px|link=http://www.iubmb.org|IUBMB - ''International Union of Biochemistry and Molecular Biology'']]<br />
[[Image:MiPsocietyLOGO.JPG|right|200px|link=http://www.mitophysiology.org|MiPsociety]]<br />
[http://www.mitophysiology.org/?MiP2014 '''<big>MiP2014 - 10<sup>th</sup> MiP''conference'': Joint IUBMB/MiP Symposium on Mitochondrial Physiology - a Point/Counterpoint Meeting</big>''']<br />
<br />
'''''International Union of Biochemistry and Molecular Biology''''' and '''Mitochondrial Physiology Society'''<br />
<br />
Obergurgl, Tyrol, Austria. 2014-Sep-08 to 12<br />
== MiP2014 - a point/counterpoint meeting ==<br />
<br />
Mitochondrial research has recently undergone a dramatic renaissance, due to numerous discoveries revealing its central role in cell death, disease pathology, aging, thermogenesis, oxidative stress, cell signalling, cellular regulation and cell bioenergetics. The aim of the small symposium is to bring together experts in mitochondrial research spanning from the biochemical and molecular level to the interactions within the cell. In addition to the latest scientific findings, new methodological developments in measuring mitochondrial function and dysfunction are presented. A point/counterpoint format is chosen to discuss selected hot topics and some fundamental methodological and conceptual issues.<br />
<br />
The expected attendance is max. 100 participants. ->> Compare [[MiP2013 Programme]]<br />
Abstracts and presentations: refer to [http://www.chem.qmul.ac.uk/iubmb/ IUBMB nomenclature]; >> [[MitoPedia Glossary: Respiratory states|MitoPedia Glossary: '''Respiratory states''']]<br />
<br />
== Programme structure ==<br />
<br />
In each of the 12 sessions a point/counterpoint introduction will be given by invited keynote speakers to provide a balanced overview and promote discussions. Short oral presentations are selected from abstracts, including student presentations. Rapid platform statements will introduce posters which contribute to a specific session. Posters are up throughout the meeting, and poster discussions are integrated into the platform sessions.<br />
<br />
=== A. Mitochondrial respiratory control === <br />
* A1. mtDNA, mt-phenotype and disease – is there biochemical and molecular-genetic evidence to explain observed associations? <br />
* A2. Fission and fusion, mitochondrial structure and function - is there a tight connection? <br />
* A3. Mitochondrial density and normalization of respiration – is there a general biochemical or molecular marker? <br />
* A4. Biochemical reserve capacity, respiratory control ratios and coupling efficiency – are conventional concepts inconsistent?<br />
<br />
=== B. Experimental protocols in mitochondrial biochemistry === <br />
* B1. Incubation media for mt-preparations and experimental temperature in functional studies of mammalian mitochondria – guided by concept or tradition? <br />
* B2. Intracellular oxygen pressure versus oxygen regimes in studies of ROS production and respiration - are mitochondria sources or sinks of ROS?<br />
* B3. Quantitative measurement of mt-membrane potential - potentiometric/fluorometric. <br />
* B4. Instrumental platforms in mitochondrial physiology – industrial presentations.<br />
<br />
=== C. Towards quality assurance === <br />
* C1. Do blood cells versus muscle biopsies provide valid models for diagnosis of mitochondrial pathologies? <br />
* C2. Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
* C3. From availability and quality control of chemicals to mitochondrial respiratory protocols – who controls? <br />
* C4. Standards for the diagnosis of mitochondrial pathologies – a challenge for collaboration with mitochondrial organizations?<br />
Particularly in view of Subtopic C, it may be attractive for mitochondrial organizations to send and support delegates. The conference will, therefore, provide a unique forum for international networking, and may inspire new national groups to be formed in contact with established mitochondrial organizations:<br />
>> '''[[MitoGlobal_Organizations]]'''<br />
<br />
== MiP2014 preliminary list of participants ==<br />
<br />
in progress<br />
<br />
{| class="wikitable"<br />
|-<br />
! Name !! Institution !! Preliminary title <br />
|-<br />
| [[Ali SS|Ali Sameh S]]||Zewail City for Science and Technology, ET||Oxygen-consuming and ROS-producing activities in synaptosomes.<br />
|-<br />
| [[Bauer CM|Bauer Christiane Maria]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Borutaite_V|Borutaite Vilmante]]||Lithuanian University of Health Sciences, Kaunas, LT||Controversies in testing integrity of mitochondrial membranes.<br />
|-<br />
| [[Brown D|Brown David]]||East Carolina University, NC, US||<br />
|-<br />
| [[Contreras-Ferrat A|Contreras-Ferrat Ariel]]||University of Chile, CL||<br />
|-<br />
| [[Coen P|Coen Paul]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||Does calorie restriction improve or worsen skeletal muscle mitochondrial performance?<br />
|-<br />
| [[Dohlmann T|Dohlmann Tine]]||University of Copenhagen, DK||<br />
|-<br />
| [[Elmer E|Elmer Eskil]]||Wallenberg Neuroscience Center, Lund, SE||Ex vivo respiratory capacity of human blood cells - a SUITable application of the O2k Oxygraph / A novel pharmacological approach to counteract metabolic crisis.<br />
|-<br />
| [[Felding-Habermann B|Felding-Habermann Brunhilde]]||The Scripps Research Institute, La Jolla, CA, US||Mitochondrial functions in breast cancer progression.<br />
|-<br />
| [[Goodpaster BH|Goodpaster Bret]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Gnaiger E|Gnaiger Erich]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Grefte S|Grefte Sander]]||Radboud University Nijmegen Medical Centre, Nijmegen, NL||Pathophysiology of mitochondrial (dys)function in skeletal muscle.<br />
|-<br />
| [[Hahn D|Hahn Dagmar]]||Inselspital Bern, CH||Validation of oxygen consumption measurements in muscle and fibroblasts from patients with mitochondrial diseases. <br />
|-<br />
| [[Hand SC|Hand Steven C]]||Louisiana State University, LA, US||Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
|-<br />
| [[Harrison DK|Harrison David K]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Hepple_RT| Hepple Russel T.]]||McGill University, Montreal, CA||Mitochondrial involvement in aging muscle: cause and effect(or).<br />
|-<br />
| [[Hey-Mogensen M|Hey-Mogensen Martin]]||Novo Nordisk A/S Diabetes NBEs & Obesity Biology, Måløv, DK||<br />
|-<br />
| [[Jana Prado F|Jana Prado Fabian]]||University of Chile, CL||<br />
|-<br />
| [[Kane DA| Kane Daniel A.]]||St. Francis Xavier University, Antigonish, CA||Mitochondrial shuttles in muscle.<br />
|-<br />
| [[Kraunsoe R|Kraunsoe Regitze]]||University of Copenhagen, DK||<br />
|-<br />
| [[Koopman WJ|Koopman Werner J]]||Radboud University Nijmegen Medical Centre, NL||Redox regulation of mitochondrial dynamics.<br />
|-<br />
| [[Kowaltowski AJ|Kowaltowski Alicia J.]]||University of Sao Paulo, BR||<br />
|-<br />
| [[Laner V|Laner Verena]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Larsen S|Larsen Steen]]||University of Copenhagen, DK||Normalization of respiration.<br />
|-<br />
| [[Larsson NG|Göran-Larsson Nils]]||Max Planck Institute for the Biology of Ageing, Cologne, DE||Mouse models with mtDNA mutations.<br />
|-<br />
| [[Ojuka E|Ojuka Edward]]||University of Cape Town, ZA||Fission and fusion, mitochondrial structure and function - is there a tight connection?<br />
|-<br />
| [[Perry CG|Perry Christopher G]]||York University, Ontario, CA||Re-visiting critical experimental parameters to control when measuring respiratory kinetics in permeabilized muscle fibres.<br />
|-<br />
| [[Pesta D|Pesta Dominik]]||Yale University School of Medicine, CT, US||Dysregulation of muscle ATP synthesis in hypophosphatemic NaPi2a knockout mice and in a patient with hypophosphatemia due to a mutation in NaPi2c. <br />
|- <br />
| [[Picard_M|Picard Martin]]||University of Pennsylvania, PA, US||Quantitative regulation of nuclear gene expression by mitochondrial DNA<br />
heteroplasmy.<br />
|-<br />
| [[Pecina P|Pecina Petr]]||Academy of Sciences of the Czech Republic, Prague, CZ||The use of lymphocytes for diagnostics of mitochondrial disorders.<br />
|-<br />
| [[Purhonen J|Purhonen Janne]]||University Helsinki, FI||<br />
|-<br />
| [[Ristow M|Ristow Michael]]||ETH Zurich, CH||Promoting health and lifespan by increasing mitochondrial ROS formation.<br />
|-<br />
| [[Shirihai O|Shirihai Orian]]||The Shirihai Lab at Boston University, MA, US||<br />
|-<br />
| [[Skulachev VP|Skulachev Vladimir]]||Lomonosov Moscow State University, Moscow, RU||Mitochondria are sources of ROS rather than sinks: Physiological effects of mitochondria-targeted antioxidants.<br />
|-<br />
| [[Smith S|Smith Steven]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Sparks LM|Sparks Lauren M]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||The potential role of mitochondrial efficiency and energetics in the endurance-trained phenotype of NAMPT Transgenic (Tg) mice.<br />
|-<br />
| [[Starkov AA|Starkov Anatoly]]||Weill Medical College Cornell University, NY, US||<br />
|-<br />
| [[Stiban J|Stiban Johnny]]||Birzeit University, Ramallah, PS||Very long chain ceramides interfere with C16-ceramide-induced channel formation: A plausible mechanism for regulating the initiation of intrinsic apoptosis.<br />
|-<br />
| [[Zorzano A|Zorzano Antonio]]||Universitat de Barcelona, ES||Metabolic implications of mitofusin 2 dysfunction.<br />
|-<br />
| [[Sumbalova Z|Sumbalova Zuzana]]||Comenius University, Bratislava, SK||<br />
|}<br />
<br />
== Sponsors ==<br />
<br />
[[Image:OROBOROS Oxygraph-2k Logo.jpg|left|200px|link=http://www.oroboros.at|OROBOROS]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[Image:HAMLogoRGB.jpg|left|200px|link=http://www.hamilton.ch|Hamilton]]<br />
<br />
<br />
<br />
<br />
<br />
[[Image:Hansatech.jpg|left|200px|link=http://www.hansatech-instruments.com/|Hansatech]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
== Venue ==<br />
<br />
The symposium will be hosted in the '''University Center in Obergurgl''' 100 km from Innsbruck. The closest airport is in Innsbruck. Participants arriving at Munich airport will take the train or an airport shuttle to Innsbruck. We provide support in arranging the transfer between Munich and Innsbruck. A bus transfer is organized between Innsbruck and Obergurgl.<br />
<br />
The Obergurgl University Center is located at an altitude of 1940 meters above sea-level. The historical buildings, carefully refurbished and equipped with the latest technical systems, can accommodate around 70 persons in 43 bedrooms and includes conference rooms, the Alpine Research Center, laboratory, sports medicine, lounges and the restaurant. All rooms are equipped with an Internet connection. Additional participants are accommodated in smaller guest houses in 5 minutes walking distance. Meals are served for all participants in the University Center. Two conference rooms for a total of 200 persons and three small rooms for round table discussions are available. There is enough space for poster presentations and instrument exhibitions. The cosy bar and lounge area with an open fire place adds to the familiar atmosphere and supports the interactive spirit of the symposium.<br />
<br />
The small village of Obergurgl is situated in an impressive landscape at about 1900 m above sea level in the Ötz valley. It is surrounded by mountains, offering an exceptional view to the Ötz valley glaciers. During summer time the area is famous for hiking and for skiing in winter time. On the doorstep: one of Europe´s finest Biosphere Parks. The unique surroundings offer the possibility of combining scientific discussions while exploring the alpine environment during the famous “walks and talks”. The informal atmosphere of the workshop encourages also students and young post-docs to participate actively in scientific discussions and to develop future collaborations.<br />
<br />
University Centre Obergurgl, Gaisbergweg 5, 6456 Obergurgl, Tyrol, Austria.<br />
Tel.: +43 (0) 512 507 37201 | Fax: +43 (0) 512 507 37400 | Email: obergurgl@uibk.ac.at<br />
<br />
== International programme committee ==<br />
The international programme committee will provide scientific input by active participation, nominate further invited speakers to ensure balanced point/counterpoint introductions, and select the abstracts as a basis for participation (restricted number of participants), for oral 10+5 min presentations, and for flash presentations.<br />
<br />
Chair:<br />
* [[Gnaiger E|Gnaiger Erich]], Medical University Innsbruck, AT. - erich.gnaiger@mitophysiology.org<br />
Members:<br />
* [[Ali SS|Ali Sameh S]], Helmy Institute of Medical Sciences, Giza, Egypt<br />
* [[Borutaite V|Borutaite Vilma]], Lithuanian University of Health Sciences Kaunas, LT<br />
* [[Neurocon|Chakrabarti Sasanka]], Institute of Post-graduate Medical Education & Research, Kolkatta, India<br />
* [[Chinese Society of Mitochondrial Research and Medicine|Chen Quan]], Chinese Academy of Sciences, Nankai University, Beijing <br />
* [[Dela F|Dela Flemming]], University of Copenhagen, DK <br />
* [[Duchen MR|Duchen Michael R]], University College London, UK<br />
* [[Elmer E|Elmér Eskil]], Lund University, SE <br />
* [[Hand SC|Hand Steven]], Louisiana State University, LA, US<br />
* [[Hickey AJ|Hickey Anthony J]], University of Auckland, NZ<br />
* [[Koopman WJ|Koopman Werner J]], Radboud Univ Nijmegen Medical Centre, NL<br />
* [[Tanaka M|Tanaka Masashi]], Toyko Metropolitan Institute of Gerontology, JP<br />
* [[Neufer PD|Neufer P Darrell]], East Carolina University, Greenville, NC, US<br />
* [[Ojuka E|Ojuka Edward]], University of Cape Town, South Africa<br />
* [[Pecina P|Pecina Petr]], Academy of Sciences of the Czech Republic, CZ<br />
* [[Perry CG|Perry Christopher G]], York University, Toronto, Canada<br />
* [[Rossignol R|Rossignol Rodrigue]], Université Victor Segalen-Bordeaux, FR<br />
* [[Vercesi AE|Vercesi Anibal E]], State University of Campinas, Sao Paulo, Brazil<br />
* [[Taiwan Society for Mitochondrial Research and Medicine|Wei Yau-Huei]], Mackay Medical College, New Taipei City, Taiwan<br />
* [[Zorzano A|Zorzano Antonio]], Universitat de Barcelona, Spain<br />
<br />
== Information for invited speakers==<br />
<br />
MiP2014 will be held at the University Centre Obergurgl, with maximum capacity of 124 persons and a price per person per night of € 65.- including a shared double room, breakfast, and dinner. MiP''conferences'' are organized as small meetings facilitating communication between senior and junior participants. In the tradition of MiP''conferences'', we are asking invited speakers to cooperate and try to receive local support for joining us at the prestigious Mitochondrial Physiology Conference (limited to 124 participants). Support will be provided to students and young investigators.<br />
<br />
== Post-conference O2k-Workshop ==<br />
<br />
A [http://www.oroboros.at/?IOC_2014-09_obergurgl post-conference workshop] is organized on the same venue, for hands-on training in mitochondrial respiratory physiology, introducing the combined measurement of high-resolution respirometry and fluorometry (OROBOROS INSTRUMENTS). Since participants come from all-over-the-world, including many students, the combination of the symposium with the workshop makes travelling more economical for the maximum of 24 workshop-participants. The post-conference workshop is financially completely separate from the symposium.<br />
<br />
[[File:MitoGlobal.jpg|left|40px|MitoGlobal]]<br />
== MiP''events'' ==<br />
are listed as '''[[MitoGlobal_Events]]'''.<br />
<br />
<br />
<br />
<br />
<br />
[[Category:MitoPedia]]<br />
[[Category:All]]</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=Smith_S&diff=58612Smith S2014-04-18T09:01:40Z<p>Bauer Christiane: Created page with "{{Person |lastname=Smith |firstname=Steven |title=M.D., Chief Scientific Officer, Professor |institution= Translational Research Institute for Metabolism and Diabetes Florida H..."</p>
<hr />
<div>{{Person<br />
|lastname=Smith<br />
|firstname=Steven<br />
|title=M.D., Chief Scientific Officer, Professor<br />
|institution=<br />
<br />
Translational Research Institute for Metabolism and Diabetes<br />
<br />
Florida Hospital/Sanford Burnham<br />
|address=301 East Princeton Street<br />
|area code=32804<br />
|city=Orlando<br />
|state=Florida (FL)<br />
|country=USA<br />
|mailaddress=steven.r.smith.md@flhosp.org<br />
|weblink=http://www.tri-md.org/content/steven-r-smith-md-scientific-director<br />
}}<br />
{{Labelingperson<br />
|topics=<br />
}}</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MiP2014&diff=58611MiP20142014-04-18T08:52:10Z<p>Bauer Christiane: /* MiP2014 preliminary participants */</p>
<hr />
<div>[[Image:IUBMB-logo.gif|left|100px|link=http://www.iubmb.org|IUBMB - ''International Union of Biochemistry and Molecular Biology'']]<br />
[[Image:MiPsocietyLOGO.JPG|right|200px|link=http://www.mitophysiology.org|MiPsociety]]<br />
[http://www.mitophysiology.org/?MiP2014 '''<big>MiP2014 - 10<sup>th</sup> MiP''conference'': Joint IUBMB/MiP Symposium on Mitochondrial Physiology - a Point/Counterpoint Meeting</big>''']<br />
<br />
'''''International Union of Biochemistry and Molecular Biology''''' and '''Mitochondrial Physiology Society'''<br />
<br />
Obergurgl, Tyrol, Austria. 2014-Sep-08 to 12<br />
== MiP2014 - a point/counterpoint meeting ==<br />
<br />
Mitochondrial research has recently undergone a dramatic renaissance, due to numerous discoveries revealing its central role in cell death, disease pathology, aging, thermogenesis, oxidative stress, cell signalling, cellular regulation and cell bioenergetics. The aim of the small symposium is to bring together experts in mitochondrial research spanning from the biochemical and molecular level to the interactions within the cell. In addition to the latest scientific findings, new methodological developments in measuring mitochondrial function and dysfunction are presented. A point/counterpoint format is chosen to discuss selected hot topics and some fundamental methodological and conceptual issues.<br />
<br />
The expected attendance is max. 100 participants. ->> Compare [[MiP2013 Programme]]<br />
Abstracts and presentations: refer to [http://www.chem.qmul.ac.uk/iubmb/ IUBMB nomenclature]; >> [[MitoPedia Glossary: Respiratory states|MitoPedia Glossary: '''Respiratory states''']]<br />
<br />
== Programme structure ==<br />
<br />
In each of the 12 sessions a point/counterpoint introduction will be given by invited keynote speakers to provide a balanced overview and promote discussions. Short oral presentations are selected from abstracts, including student presentations. Rapid platform statements will introduce posters which contribute to a specific session. Posters are up throughout the meeting, and poster discussions are integrated into the platform sessions.<br />
<br />
=== A. Mitochondrial respiratory control === <br />
* A1. mtDNA, mt-phenotype and disease – is there biochemical and molecular-genetic evidence to explain observed associations? <br />
* A2. Fission and fusion, mitochondrial structure and function - is there a tight connection? <br />
* A3. Mitochondrial density and normalization of respiration – is there a general biochemical or molecular marker? <br />
* A4. Biochemical reserve capacity, respiratory control ratios and coupling efficiency – are conventional concepts inconsistent?<br />
<br />
=== B. Experimental protocols in mitochondrial biochemistry === <br />
* B1. Incubation media for mt-preparations and experimental temperature in functional studies of mammalian mitochondria – guided by concept or tradition? <br />
* B2. Intracellular oxygen pressure versus oxygen regimes in studies of ROS production and respiration - are mitochondria sources or sinks of ROS?<br />
* B3. Quantitative measurement of mt-membrane potential - potentiometric/fluorometric. <br />
* B4. Instrumental platforms in mitochondrial physiology – industrial presentations.<br />
<br />
=== C. Towards quality assurance === <br />
* C1. Do blood cells versus muscle biopsies provide valid models for diagnosis of mitochondrial pathologies? <br />
* C2. Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
* C3. From availability and quality control of chemicals to mitochondrial respiratory protocols – who controls? <br />
* C4. Standards for the diagnosis of mitochondrial pathologies – a challenge for collaboration with mitochondrial organizations?<br />
Particularly in view of Subtopic C, it may be attractive for mitochondrial organizations to send and support delegates. The conference will, therefore, provide a unique forum for international networking, and may inspire new national groups to be formed in contact with established mitochondrial organizations:<br />
>> '''[[MitoGlobal_Organizations]]'''<br />
<br />
== MiP2014 preliminary participants ==<br />
<br />
in progress<br />
<br />
{| class="wikitable"<br />
|-<br />
! Name !! Institution !! Preliminary title <br />
|-<br />
| [[Ali SS|Ali Sameh S]]||Zewail City for Science and Technology, ET||Oxygen-consuming and ROS-producing activities in synaptosomes.<br />
|-<br />
| [[Bauer CM|Bauer Christiane Maria]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Borutaite_V|Borutaite Vilmante]]||Lithuanian University of Health Sciences, Kaunas, LT||Controversies in testing integrity of mitochondrial membranes.<br />
|-<br />
| [[Brown D|Brown David]]||East Carolina University, NC, US||<br />
|-<br />
| [[Contreras-Ferrat A|Contreras-Ferrat Ariel]]||University of Chile, CL||<br />
|-<br />
| [[Coen P|Coen Paul]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||Does calorie restriction improve or worsen skeletal muscle mitochondrial performance?<br />
|-<br />
| [[Dohlmann T|Dohlmann Tine]]||University of Copenhagen, DK||<br />
|-<br />
| [[Elmer E|Elmer Eskil]]||Wallenberg Neuroscience Center, Lund, SE||Ex vivo respiratory capacity of human blood cells - a SUITable application of the O2k Oxygraph / A novel pharmacological approach to counteract metabolic crisis.<br />
|-<br />
| [[Felding-Habermann B|Felding-Habermann Brunhilde]]||The Scripps Research Institute, La Jolla, CA, US||Mitochondrial functions in breast cancer progression.<br />
|-<br />
| [[Goodpaster BH|Goodpaster Bret]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Gnaiger E|Gnaiger Erich]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Grefte S|Grefte Sander]]||Radboud University Nijmegen Medical Centre, Nijmegen, NL||Pathophysiology of mitochondrial (dys)function in skeletal muscle.<br />
|-<br />
| [[Hahn D|Hahn Dagmar]]||Inselspital Bern, CH||Validation of Oxygen Consumption measurements in fibroblasts from patients with mitochondrial diseases.<br />
|-<br />
| [[Hand SC|Hand Steven C]]||Louisiana State University, LA, US||Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
|-<br />
| [[Harrison DK|Harrison David K]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Hepple_RT| Hepple Russel T.]]||McGill University, Montreal, CA||Mitochondrial involvement in aging muscle: cause and effect(or).<br />
|-<br />
| [[Hey-Mogensen M|Hey-Mogensen Martin]]||Novo Nordisk A/S Diabetes NBEs & Obesity Biology, Måløv, DK||<br />
|-<br />
| [[Jana Prado F|Jana Prado Fabian]]||University of Chile, CL||<br />
|-<br />
| [[Kane DA| Kane Daniel A.]]||St. Francis Xavier University, Antigonish, CA||Mitochondrial shuttles in muscle.<br />
|-<br />
| [[Kraunsoe R|Kraunsoe Regitze]]||University of Copenhagen, DK||<br />
|-<br />
| [[Koopman WJ|Koopman Werner J]]||Radboud University Nijmegen Medical Centre, NL||Redox regulation of mitochondrial dynamics.<br />
|-<br />
| [[Kowaltowski AJ|Kowaltowski Alicia J.]]||University of Sao Paulo, BR||<br />
|-<br />
| [[Laner V|Laner Verena]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Larsen S|Larsen Steen]]||University of Copenhagen, DK||Normalization of respiration.<br />
|-<br />
| [[Larsson NG|Göran-Larsson Nils]]||Max Planck Institute for the Biology of Ageing, Cologne, DE||Mouse models with mtDNA mutations.<br />
|-<br />
| [[Ojuka E|Ojuka Edward]]||University of Cape Town, ZA||Fission and fusion, mitochondrial structure and function - is there a tight connection?<br />
|-<br />
| [[Perry CG|Perry Christopher G]]||York University, Ontario, CA||Re-visiting critical experimental parameters to control when measuring respiratory kinetics in permeabilized muscle fibres.<br />
|-<br />
| [[Pesta D|Pesta Dominik]]||Yale University School of Medicine, CT, US||Dysregulation of muscle ATP synthesis in hypophosphatemic NaPi2a knockout mice and in a patient with hypophosphatemia due to a mutation in NaPi2c. <br />
|- <br />
| [[Picard_M|Picard Martin]]||University of Pennsylvania, PA, US||Quantitative regulation of nuclear gene expression by mitochondrial DNA<br />
heteroplasmy.<br />
|-<br />
| [[Pecina P|Pecina Petr]]||Academy of Sciences of the Czech Republic, Prague, CZ||The use of lymphocytes for diagnostics of mitochondrial disorders.<br />
|-<br />
| [[Purhonen J|Purhonen Janne]]||University Helsinki, FI||<br />
|-<br />
| [[Ristow M|Ristow Michael]]||ETH Zurich, CH||Promoting health and lifespan by increasing mitochondrial ROS formation.<br />
|-<br />
| [[Shirihai O|Shirihai Orian]]||The Shirihai Lab at Boston University, MA, US||<br />
|-<br />
| [[Skulachev VP|Skulachev Vladimir]]||Lomonosov Moscow State University, Moscow, RU||Mitochondria are sources of ROS rather than sinks: Physiological effects of mitochondria-targeted antioxidants.<br />
|-<br />
| [[Smith S|Smith Steven]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Sparks LM|Sparks Lauren M]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||The potential role of mitochondrial efficiency and energetics in the endurance-trained phenotype of NAMPT Transgenic (Tg) mice.<br />
|-<br />
| [[Starkov AA|Starkov Anatoly]]||Weill Medical College Cornell University, NY, US||<br />
|-<br />
| [[Stiban J|Stiban Johnny]]||Birzeit University, Ramallah, PS||Very long chain ceramides interfere with C16-ceramide-induced channel formation: A plausible mechanism for regulating the initiation of intrinsic apoptosis.<br />
|-<br />
| [[Zorzano A|Zorzano Antonio]]||Universitat de Barcelona, ES||Metabolic implications of mitofusin 2 dysfunction.<br />
|-<br />
| [[Sumbalova Z|Sumbalova Zuzana]]||Comenius University, Bratislava, SK||<br />
|}<br />
<br />
== Sponsors ==<br />
<br />
[[Image:OROBOROS Oxygraph-2k Logo.jpg|left|200px|link=http://www.oroboros.at|OROBOROS]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[Image:HAMLogoRGB.jpg|left|200px|link=http://www.hamilton.ch|Hamilton]]<br />
<br />
<br />
<br />
<br />
<br />
[[Image:Hansatech.jpg|left|200px|link=http://www.hansatech-instruments.com/|Hansatech]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
== Venue ==<br />
<br />
The symposium will be hosted in the '''University Center in Obergurgl''' 100 km from Innsbruck. The closest airport is in Innsbruck. Participants arriving at Munich airport will take the train or an airport shuttle to Innsbruck. We provide support in arranging the transfer between Munich and Innsbruck. A bus transfer is organized between Innsbruck and Obergurgl.<br />
<br />
The Obergurgl University Center is located at an altitude of 1940 meters above sea-level. The historical buildings, carefully refurbished and equipped with the latest technical systems, can accommodate around 70 persons in 43 bedrooms and includes conference rooms, the Alpine Research Center, laboratory, sports medicine, lounges and the restaurant. All rooms are equipped with an Internet connection. Additional participants are accommodated in smaller guest houses in 5 minutes walking distance. Meals are served for all participants in the University Center. Two conference rooms for a total of 200 persons and three small rooms for round table discussions are available. There is enough space for poster presentations and instrument exhibitions. The cosy bar and lounge area with an open fire place adds to the familiar atmosphere and supports the interactive spirit of the symposium.<br />
<br />
The small village of Obergurgl is situated in an impressive landscape at about 1900 m above sea level in the Ötz valley. It is surrounded by mountains, offering an exceptional view to the Ötz valley glaciers. During summer time the area is famous for hiking and for skiing in winter time. On the doorstep: one of Europe´s finest Biosphere Parks. The unique surroundings offer the possibility of combining scientific discussions while exploring the alpine environment during the famous “walks and talks”. The informal atmosphere of the workshop encourages also students and young post-docs to participate actively in scientific discussions and to develop future collaborations.<br />
<br />
University Centre Obergurgl, Gaisbergweg 5, 6456 Obergurgl, Tyrol, Austria.<br />
Tel.: +43 (0) 512 507 37201 | Fax: +43 (0) 512 507 37400 | Email: obergurgl@uibk.ac.at<br />
<br />
== International programme committee ==<br />
The international programme committee will provide scientific input by active participation, nominate further invited speakers to ensure balanced point/counterpoint introductions, and select the abstracts as a basis for participation (restricted number of participants), for oral 10+5 min presentations, and for flash presentations.<br />
<br />
Chair:<br />
* [[Gnaiger E|Gnaiger Erich]], Medical University Innsbruck, AT. - erich.gnaiger@mitophysiology.org<br />
Members:<br />
* [[Ali SS|Ali Sameh S]], Helmy Institute of Medical Sciences, Giza, Egypt<br />
* [[Borutaite V|Borutaite Vilma]], Lithuanian University of Health Sciences Kaunas, LT<br />
* [[Neurocon|Chakrabarti Sasanka]], Institute of Post-graduate Medical Education & Research, Kolkatta, India<br />
* [[Chinese Society of Mitochondrial Research and Medicine|Chen Quan]], Chinese Academy of Sciences, Nankai University, Beijing <br />
* [[Dela F|Dela Flemming]], University of Copenhagen, DK <br />
* [[Duchen MR|Duchen Michael R]], University College London, UK<br />
* [[Elmer E|Elmér Eskil]], Lund University, SE <br />
* [[Hand SC|Hand Steven]], Louisiana State University, LA, US<br />
* [[Hickey AJ|Hickey Anthony J]], University of Auckland, NZ<br />
* [[Koopman WJ|Koopman Werner J]], Radboud Univ Nijmegen Medical Centre, NL<br />
* [[Tanaka M|Tanaka Masashi]], Toyko Metropolitan Institute of Gerontology, JP<br />
* [[Neufer PD|Neufer P Darrell]], East Carolina University, Greenville, NC, US<br />
* [[Ojuka E|Ojuka Edward]], University of Cape Town, South Africa<br />
* [[Pecina P|Pecina Petr]], Academy of Sciences of the Czech Republic, CZ<br />
* [[Perry CG|Perry Christopher G]], York University, Toronto, Canada<br />
* [[Rossignol R|Rossignol Rodrigue]], Université Victor Segalen-Bordeaux, FR<br />
* [[Vercesi AE|Vercesi Anibal E]], State University of Campinas, Sao Paulo, Brazil<br />
* [[Taiwan Society for Mitochondrial Research and Medicine|Wei Yau-Huei]], Mackay Medical College, New Taipei City, Taiwan<br />
* [[Zorzano A|Zorzano Antonio]], Universitat de Barcelona, Spain<br />
<br />
== Information for invited speakers==<br />
<br />
MiP2014 will be held at the University Centre Obergurgl, with maximum capacity of 124 persons and a price per person per night of € 65.- including a shared double room, breakfast, and dinner. MiP''conferences'' are organized as small meetings facilitating communication between senior and junior participants. In the tradition of MiP''conferences'', we are asking invited speakers to cooperate and try to receive local support for joining us at the prestigious Mitochondrial Physiology Conference (limited to 124 participants). Support will be provided to students and young investigators.<br />
<br />
== Post-conference O2k-Workshop ==<br />
<br />
A [http://www.oroboros.at/?IOC_2014-09_obergurgl post-conference workshop] is organized on the same venue, for hands-on training in mitochondrial respiratory physiology, introducing the combined measurement of high-resolution respirometry and fluorometry (OROBOROS INSTRUMENTS). Since participants come from all-over-the-world, including many students, the combination of the symposium with the workshop makes travelling more economical for the maximum of 24 workshop-participants. The post-conference workshop is financially completely separate from the symposium.<br />
<br />
[[File:MitoGlobal.jpg|left|40px|MitoGlobal]]<br />
== MiP''events'' ==<br />
are listed as '''[[MitoGlobal_Events]]'''.<br />
<br />
<br />
<br />
<br />
<br />
[[Category:MitoPedia]]<br />
[[Category:All]]</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MiP2014&diff=58433MiP20142014-04-15T09:38:10Z<p>Bauer Christiane: /* Venue */</p>
<hr />
<div>[[Image:IUBMB-logo.gif|left|100px|link=http://www.iubmb.org|IUBMB - ''International Union of Biochemistry and Molecular Biology'']]<br />
[[Image:MiPsocietyLOGO.JPG|right|200px|link=http://www.mitophysiology.org|MiPsociety]]<br />
[http://www.mitophysiology.org/?MiP2014 '''<big>MiP2014 - 10<sup>th</sup> MiP''conference'': Joint IUBMB/MiP Symposium on Mitochondrial Physiology - a Point/Counterpoint Meeting</big>''']<br />
<br />
'''''International Union of Biochemistry and Molecular Biology''''' and '''Mitochondrial Physiology Society'''<br />
<br />
Obergurgl, Tyrol, Austria. 2014-Sep-08 to 12<br />
== MiP2014 - a point/counterpoint meeting ==<br />
<br />
Mitochondrial research has recently undergone a dramatic renaissance, due to numerous discoveries revealing its central role in cell death, disease pathology, aging, thermogenesis, oxidative stress, cell signalling, cellular regulation and cell bioenergetics. The aim of the small symposium is to bring together experts in mitochondrial research spanning from the biochemical and molecular level to the interactions within the cell. In addition to the latest scientific findings, new methodological developments in measuring mitochondrial function and dysfunction are presented. A point/counterpoint format is chosen to discuss selected hot topics and some fundamental methodological and conceptual issues.<br />
<br />
The expected attendance is max. 100 participants. ->> Compare [[MiP2013 Programme]]<br />
Abstracts and presentations: refer to [http://www.chem.qmul.ac.uk/iubmb/ IUBMB nomenclature]; >> [[MitoPedia Glossary: Respiratory states|MitoPedia Glossary: '''Respiratory states''']]<br />
<br />
== Programme structure ==<br />
<br />
In each of the 12 sessions a point/counterpoint introduction will be given by invited keynote speakers to provide a balanced overview and promote discussions. Short oral presentations are selected from abstracts, including student presentations. Rapid platform statements will introduce posters which contribute to a specific session. Posters are up throughout the meeting, and poster discussions are integrated into the platform sessions.<br />
<br />
=== A. Mitochondrial respiratory control === <br />
* A1. mtDNA, mt-phenotype and disease – is there biochemical and molecular-genetic evidence to explain observed associations? <br />
* A2. Fission and fusion, mitochondrial structure and function - is there a tight connection? <br />
* A3. Mitochondrial density and normalization of respiration – is there a general biochemical or molecular marker? <br />
* A4. Biochemical reserve capacity, respiratory control ratios and coupling efficiency – are conventional concepts inconsistent?<br />
<br />
=== B. Experimental protocols in mitochondrial biochemistry === <br />
* B1. Incubation media for mt-preparations and experimental temperature in functional studies of mammalian mitochondria – guided by concept or tradition? <br />
* B2. Intracellular oxygen pressure versus oxygen regimes in studies of ROS production and respiration - are mitochondria sources or sinks of ROS?<br />
* B3. Quantitative measurement of mt-membrane potential - potentiometric/fluorometric. <br />
* B4. Instrumental platforms in mitochondrial physiology – industrial presentations.<br />
<br />
=== C. Towards quality assurance === <br />
* C1. Do blood cells versus muscle biopsies provide valid models for diagnosis of mitochondrial pathologies? <br />
* C2. Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
* C3. From availability and quality control of chemicals to mitochondrial respiratory protocols – who controls? <br />
* C4. Standards for the diagnosis of mitochondrial pathologies – a challenge for collaboration with mitochondrial organizations?<br />
Particularly in view of Subtopic C, it may be attractive for mitochondrial organizations to send and support delegates. The conference will, therefore, provide a unique forum for international networking, and may inspire new national groups to be formed in contact with established mitochondrial organizations:<br />
>> '''[[MitoGlobal_Organizations]]'''<br />
<br />
== MiP2014 preliminary participants ==<br />
<br />
in progress<br />
<br />
{| class="wikitable"<br />
|-<br />
! Name !! Institution !! Preliminary title <br />
|-<br />
| [[Ali SS|Ali Sameh S]]||Zewail City for Science and Technology, ET||Oxygen-consuming and ROS-producing activities in synaptosomes.<br />
|-<br />
| [[Bauer CM|Bauer Christiane Maria]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Borutaite_V|Borutaite Vilmante]]||Lithuanian University of Health Sciences, Kaunas, LT||Controversies in testing integrity of mitochondrial membranes.<br />
|-<br />
| [[Brown D|Brown David]]||East Carolina University, NC, US||<br />
|-<br />
| [[Contreras-Ferrat A|Contreras-Ferrat Ariel]]||University of Chile, CL||<br />
|-<br />
| [[Coen P|Coen Paul]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Dohlmann T|Dohlmann Tine]]||University of Copenhagen, DK||<br />
|-<br />
| [[Elmer E|Elmer Eskil]]||Wallenberg Neuroscience Center, Lund, SE||Ex vivo respiratory capacity of human blood cells - a SUITable application of the O2k Oxygraph / A novel pharmacological approach to counteract metabolic crisis.<br />
|-<br />
| [[Felding-Habermann B|Felding-Habermann Brunhilde]]||The Scripps Research Institute, La Jolla, CA, US||Mitochondrial functions in breast cancer progression.<br />
|-<br />
| [[Goodpaster BH|Goodpaster Bret]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Gnaiger E|Gnaiger Erich]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Grefte S|Grefte Sander]]||Radboud University Nijmegen Medical Centre, Nijmegen, NL||Pathophysiology of mitochondrial (dys)function in skeletal muscle.<br />
|-<br />
| [[Hahn D|Hahn Dagmar]]||Inselspital Bern, CH||Validation of Oxygen Consumption measurements in fibroblasts from patients with mitochondrial diseases.<br />
|-<br />
| [[Hand SC|Hand Steven C]]||Louisiana State University, LA, US||Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
|-<br />
| [[Harrison DK|Harrison David K]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Hepple_RT| Hepple Russel T.]]||McGill University, Montreal, CA||Mitochondrial involvement in aging muscle: cause and effect(or).<br />
|-<br />
| [[Hey-Mogensen M|Hey-Mogensen Martin]]||Novo Nordisk A/S Diabetes NBEs & Obesity Biology, Måløv, DK||<br />
|-<br />
| [[Jana Prado F|Jana Prado Fabian]]||University of Chile, CL||<br />
|-<br />
| [[Kane DA| Kane Daniel A.]]||St. Francis Xavier University, Antigonish, CA||Mitochondrial shuttles in muscle.<br />
|-<br />
| [[Kraunsoe R|Kraunsoe Regitze]]||University of Copenhagen, DK||<br />
|-<br />
| [[Koopman WJ|Koopman Werner J]]||Radboud University Nijmegen Medical Centre, NL||Redox regulation of mitochondrial dynamics.<br />
|-<br />
| [[Kowaltowski AJ|Kowaltowski Alicia J.]]||University of Sao Paulo, BR||<br />
|-<br />
| [[Laner V|Laner Verena]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Larsen S|Larsen Steen]]||University of Copenhagen, DK||Normalization of respiration.<br />
|-<br />
| [[Larsson NG|Göran-Larsson Nils]]||Max Planck Institute for the Biology of Ageing, Cologne, DE||Mouse models with mtDNA mutations.<br />
|-<br />
| [[Ojuka E|Ojuka Edward]]||University of Cape Town, ZA||Fission and fusion, mitochondrial structure and function - is there a tight connection?<br />
|-<br />
| [[Perry CG|Perry Christopher G]]||York University, Ontario, CA||Re-visiting critical experimental parameters to control when measuring respiratory kinetics in permeabilized muscle fibres.<br />
|-<br />
| [[Pesta D|Pesta Dominik]]||Yale University School of Medicine, CT, US||Dysregulation of muscle ATP synthesis in hypophosphatemic NaPi2a knockout mice and in a patient with hypophosphatemia due to a mutation in NaPi2c. <br />
|- <br />
| [[Picard_M|Picard Martin]]||University of Pennsylvania, PA, US||Quantitative regulation of nuclear gene expression by mitochondrial DNA<br />
heteroplasmy.<br />
|-<br />
| [[Pecina P|Pecina Petr]]||Academy of Sciences of the Czech Republic, Prague, CZ||The use of lymphocytes for diagnostics of mitochondrial disorders.<br />
|-<br />
| [[Purhonen J|Purhonen Janne]]||University Helsinki, FI||<br />
|-<br />
| [[Ristow M|Ristow Michael]]||ETH Zurich, CH||Promoting health and lifespan by increasing mitochondrial ROS formation.<br />
|-<br />
| [[Shirihai O|Shirihai Orian]]||The Shirihai Lab at Boston University, MA, US||<br />
|-<br />
| [[Skulachev VP|Skulachev Vladimir]]||Lomonosov Moscow State University, Moscow, RU||Mitochondria are sources of ROS rather than sinks: Physiological effects of mitochondria-targeted antioxidants.<br />
|-<br />
| [[Starkov AA|Starkov Anatoly]]||Weill Medical College Cornell University, NY, US||<br />
|-<br />
| [[Stiban J|Stiban Johnny]]||Birzeit University, Ramallah, PS||Very long chain ceramides interfere with C16-ceramide-induced channel formation: A plausible mechanism for regulating the initiation of intrinsic apoptosis.<br />
|-<br />
| [[Zorzano A|Zorzano Antonio]]||Universitat de Barcelona, ES||Metabolic implications of mitofusin 2 dysfunction.<br />
|-<br />
| [[Sumbalova Z|Sumbalova Zuzana]]||Comenius University, Bratislava, SK||<br />
|}<br />
<br />
== Sponsors ==<br />
<br />
[[Image:OROBOROS Oxygraph-2k Logo.jpg|left|200px|link=http://www.oroboros.at|OROBOROS]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[Image:HAMLogoRGB.jpg|left|200px|link=http://www.hamilton.ch|Hamilton]]<br />
<br />
<br />
<br />
<br />
<br />
[[Image:Hansatech.jpg|left|200px|link=http://www.hansatech-instruments.com/|Hansatech]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
== Venue ==<br />
<br />
The symposium will be hosted in the '''University Center in Obergurgl''' 100 km from Innsbruck. The closest airport is in Innsbruck. Participants arriving at Munich airport will take the train or an airport shuttle to Innsbruck. We provide support in arranging the transfer between Munich and Innsbruck. A bus transfer is organized between Innsbruck and Obergurgl.<br />
<br />
The Obergurgl University Center is located at an altitude of 1940 meters above sea-level. The historical buildings, carefully refurbished and equipped with the latest technical systems, can accommodate around 70 persons in 43 bedrooms and includes conference rooms, the Alpine Research Center, laboratory, sports medicine, lounges and the restaurant. All rooms are equipped with an Internet connection. Additional participants are accommodated in smaller guest houses in 5 minutes walking distance. Meals are served for all participants in the University Center. Two conference rooms for a total of 200 persons and three small rooms for round table discussions are available. There is enough space for poster presentations and instrument exhibitions. The cosy bar and lounge area with an open fire place adds to the familiar atmosphere and supports the interactive spirit of the symposium.<br />
<br />
The small village of Obergurgl is situated in an impressive landscape at about 1900 m above sea level in the Ötz valley. It is surrounded by mountains, offering an exceptional view to the Ötz valley glaciers. During summer time the area is famous for hiking and for skiing in winter time. On the doorstep: one of Europe´s finest Biosphere Parks. The unique surroundings offer the possibility of combining scientific discussions while exploring the alpine environment during the famous “walks and talks”. The informal atmosphere of the workshop encourages also students and young post-docs to participate actively in scientific discussions and to develop future collaborations.<br />
<br />
University Centre Obergurgl, Gaisbergweg 5, 6456 Obergurgl, Tyrol, Austria.<br />
Tel.: +43 (0) 512 507 37201 | Fax: +43 (0) 512 507 37400 | Email: obergurgl@uibk.ac.at<br />
<br />
== International programme committee ==<br />
The international programme committee will provide scientific input by active participation, nominate further invited speakers to ensure balanced point/counterpoint introductions, and select the abstracts as a basis for participation (restricted number of participants), for oral 10+5 min presentations, and for flash presentations.<br />
<br />
Chair:<br />
* [[Gnaiger E|Gnaiger Erich]], Medical University Innsbruck, AT. - erich.gnaiger@mitophysiology.org<br />
Members:<br />
* [[Ali SS|Ali Sameh S]], Helmy Institute of Medical Sciences, Giza, Egypt<br />
* [[Borutaite V|Borutaite Vilma]], Lithuanian University of Health Sciences Kaunas, LT<br />
* [[Neurocon|Chakrabarti Sasanka]], Institute of Post-graduate Medical Education & Research, Kolkatta, India<br />
* [[Chinese Society of Mitochondrial Research and Medicine|Chen Quan]], Chinese Academy of Sciences, Nankai University, Beijing <br />
* [[Dela F|Dela Flemming]], University of Copenhagen, DK <br />
* [[Duchen MR|Duchen Michael R]], University College London, UK<br />
* [[Elmer E|Elmér Eskil]], Lund University, SE <br />
* [[Hand SC|Hand Steven]], Louisiana State University, LA, US<br />
* [[Hickey AJ|Hickey Anthony J]], University of Auckland, NZ<br />
* [[Koopman WJ|Koopman Werner J]], Radboud Univ Nijmegen Medical Centre, NL<br />
* [[Tanaka M|Tanaka Masashi]], Toyko Metropolitan Institute of Gerontology, JP<br />
* [[Neufer PD|Neufer P Darrell]], East Carolina University, Greenville, NC, US<br />
* [[Ojuka E|Ojuka Edward]], University of Cape Town, South Africa<br />
* [[Pecina P|Pecina Petr]], Academy of Sciences of the Czech Republic, CZ<br />
* [[Perry CG|Perry Christopher G]], York University, Toronto, Canada<br />
* [[Rossignol R|Rossignol Rodrigue]], Université Victor Segalen-Bordeaux, FR<br />
* [[Vercesi AE|Vercesi Anibal E]], State University of Campinas, Sao Paulo, Brazil<br />
* [[Taiwan Society for Mitochondrial Research and Medicine|Wei Yau-Huei]], Mackay Medical College, New Taipei City, Taiwan<br />
* [[Zorzano A|Zorzano Antonio]], Universitat de Barcelona, Spain<br />
<br />
== Information for invited speakers==<br />
<br />
MiP2014 will be held at the University Centre Obergurgl, with maximum capacity of 124 persons and a price per person per night of € 65.- including a shared double room, breakfast, and dinner. MiP''conferences'' are organized as small meetings facilitating communication between senior and junior participants. In the tradition of MiP''conferences'', we are asking invited speakers to cooperate and try to receive local support for joining us at the prestigious Mitochondrial Physiology Conference (limited to 124 participants). Support will be provided to students and young investigators.<br />
<br />
== Post-conference O2k-Workshop ==<br />
<br />
A [http://www.oroboros.at/?IOC_2014-09_obergurgl post-conference workshop] is organized on the same venue, for hands-on training in mitochondrial respiratory physiology, introducing the combined measurement of high-resolution respirometry and fluorometry (OROBOROS INSTRUMENTS). Since participants come from all-over-the-world, including many students, the combination of the symposium with the workshop makes travelling more economical for the maximum of 24 workshop-participants. The post-conference workshop is financially completely separate from the symposium.<br />
<br />
[[File:MitoGlobal.jpg|left|40px|MitoGlobal]]<br />
== MiP''events'' ==<br />
are listed as '''[[MitoGlobal_Events]]'''.<br />
<br />
<br />
<br />
<br />
<br />
[[Category:MitoPedia]]<br />
[[Category:All]]</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MiP2014&diff=58432MiP20142014-04-15T09:37:50Z<p>Bauer Christiane: /* Sponsors */</p>
<hr />
<div>[[Image:IUBMB-logo.gif|left|100px|link=http://www.iubmb.org|IUBMB - ''International Union of Biochemistry and Molecular Biology'']]<br />
[[Image:MiPsocietyLOGO.JPG|right|200px|link=http://www.mitophysiology.org|MiPsociety]]<br />
[http://www.mitophysiology.org/?MiP2014 '''<big>MiP2014 - 10<sup>th</sup> MiP''conference'': Joint IUBMB/MiP Symposium on Mitochondrial Physiology - a Point/Counterpoint Meeting</big>''']<br />
<br />
'''''International Union of Biochemistry and Molecular Biology''''' and '''Mitochondrial Physiology Society'''<br />
<br />
Obergurgl, Tyrol, Austria. 2014-Sep-08 to 12<br />
== MiP2014 - a point/counterpoint meeting ==<br />
<br />
Mitochondrial research has recently undergone a dramatic renaissance, due to numerous discoveries revealing its central role in cell death, disease pathology, aging, thermogenesis, oxidative stress, cell signalling, cellular regulation and cell bioenergetics. The aim of the small symposium is to bring together experts in mitochondrial research spanning from the biochemical and molecular level to the interactions within the cell. In addition to the latest scientific findings, new methodological developments in measuring mitochondrial function and dysfunction are presented. A point/counterpoint format is chosen to discuss selected hot topics and some fundamental methodological and conceptual issues.<br />
<br />
The expected attendance is max. 100 participants. ->> Compare [[MiP2013 Programme]]<br />
Abstracts and presentations: refer to [http://www.chem.qmul.ac.uk/iubmb/ IUBMB nomenclature]; >> [[MitoPedia Glossary: Respiratory states|MitoPedia Glossary: '''Respiratory states''']]<br />
<br />
== Programme structure ==<br />
<br />
In each of the 12 sessions a point/counterpoint introduction will be given by invited keynote speakers to provide a balanced overview and promote discussions. Short oral presentations are selected from abstracts, including student presentations. Rapid platform statements will introduce posters which contribute to a specific session. Posters are up throughout the meeting, and poster discussions are integrated into the platform sessions.<br />
<br />
=== A. Mitochondrial respiratory control === <br />
* A1. mtDNA, mt-phenotype and disease – is there biochemical and molecular-genetic evidence to explain observed associations? <br />
* A2. Fission and fusion, mitochondrial structure and function - is there a tight connection? <br />
* A3. Mitochondrial density and normalization of respiration – is there a general biochemical or molecular marker? <br />
* A4. Biochemical reserve capacity, respiratory control ratios and coupling efficiency – are conventional concepts inconsistent?<br />
<br />
=== B. Experimental protocols in mitochondrial biochemistry === <br />
* B1. Incubation media for mt-preparations and experimental temperature in functional studies of mammalian mitochondria – guided by concept or tradition? <br />
* B2. Intracellular oxygen pressure versus oxygen regimes in studies of ROS production and respiration - are mitochondria sources or sinks of ROS?<br />
* B3. Quantitative measurement of mt-membrane potential - potentiometric/fluorometric. <br />
* B4. Instrumental platforms in mitochondrial physiology – industrial presentations.<br />
<br />
=== C. Towards quality assurance === <br />
* C1. Do blood cells versus muscle biopsies provide valid models for diagnosis of mitochondrial pathologies? <br />
* C2. Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
* C3. From availability and quality control of chemicals to mitochondrial respiratory protocols – who controls? <br />
* C4. Standards for the diagnosis of mitochondrial pathologies – a challenge for collaboration with mitochondrial organizations?<br />
Particularly in view of Subtopic C, it may be attractive for mitochondrial organizations to send and support delegates. The conference will, therefore, provide a unique forum for international networking, and may inspire new national groups to be formed in contact with established mitochondrial organizations:<br />
>> '''[[MitoGlobal_Organizations]]'''<br />
<br />
== MiP2014 preliminary participants ==<br />
<br />
in progress<br />
<br />
{| class="wikitable"<br />
|-<br />
! Name !! Institution !! Preliminary title <br />
|-<br />
| [[Ali SS|Ali Sameh S]]||Zewail City for Science and Technology, ET||Oxygen-consuming and ROS-producing activities in synaptosomes.<br />
|-<br />
| [[Bauer CM|Bauer Christiane Maria]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Borutaite_V|Borutaite Vilmante]]||Lithuanian University of Health Sciences, Kaunas, LT||Controversies in testing integrity of mitochondrial membranes.<br />
|-<br />
| [[Brown D|Brown David]]||East Carolina University, NC, US||<br />
|-<br />
| [[Contreras-Ferrat A|Contreras-Ferrat Ariel]]||University of Chile, CL||<br />
|-<br />
| [[Coen P|Coen Paul]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Dohlmann T|Dohlmann Tine]]||University of Copenhagen, DK||<br />
|-<br />
| [[Elmer E|Elmer Eskil]]||Wallenberg Neuroscience Center, Lund, SE||Ex vivo respiratory capacity of human blood cells - a SUITable application of the O2k Oxygraph / A novel pharmacological approach to counteract metabolic crisis.<br />
|-<br />
| [[Felding-Habermann B|Felding-Habermann Brunhilde]]||The Scripps Research Institute, La Jolla, CA, US||Mitochondrial functions in breast cancer progression.<br />
|-<br />
| [[Goodpaster BH|Goodpaster Bret]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Gnaiger E|Gnaiger Erich]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Grefte S|Grefte Sander]]||Radboud University Nijmegen Medical Centre, Nijmegen, NL||Pathophysiology of mitochondrial (dys)function in skeletal muscle.<br />
|-<br />
| [[Hahn D|Hahn Dagmar]]||Inselspital Bern, CH||Validation of Oxygen Consumption measurements in fibroblasts from patients with mitochondrial diseases.<br />
|-<br />
| [[Hand SC|Hand Steven C]]||Louisiana State University, LA, US||Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
|-<br />
| [[Harrison DK|Harrison David K]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Hepple_RT| Hepple Russel T.]]||McGill University, Montreal, CA||Mitochondrial involvement in aging muscle: cause and effect(or).<br />
|-<br />
| [[Hey-Mogensen M|Hey-Mogensen Martin]]||Novo Nordisk A/S Diabetes NBEs & Obesity Biology, Måløv, DK||<br />
|-<br />
| [[Jana Prado F|Jana Prado Fabian]]||University of Chile, CL||<br />
|-<br />
| [[Kane DA| Kane Daniel A.]]||St. Francis Xavier University, Antigonish, CA||Mitochondrial shuttles in muscle.<br />
|-<br />
| [[Kraunsoe R|Kraunsoe Regitze]]||University of Copenhagen, DK||<br />
|-<br />
| [[Koopman WJ|Koopman Werner J]]||Radboud University Nijmegen Medical Centre, NL||Redox regulation of mitochondrial dynamics.<br />
|-<br />
| [[Kowaltowski AJ|Kowaltowski Alicia J.]]||University of Sao Paulo, BR||<br />
|-<br />
| [[Laner V|Laner Verena]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Larsen S|Larsen Steen]]||University of Copenhagen, DK||Normalization of respiration.<br />
|-<br />
| [[Larsson NG|Göran-Larsson Nils]]||Max Planck Institute for the Biology of Ageing, Cologne, DE||Mouse models with mtDNA mutations.<br />
|-<br />
| [[Ojuka E|Ojuka Edward]]||University of Cape Town, ZA||Fission and fusion, mitochondrial structure and function - is there a tight connection?<br />
|-<br />
| [[Perry CG|Perry Christopher G]]||York University, Ontario, CA||Re-visiting critical experimental parameters to control when measuring respiratory kinetics in permeabilized muscle fibres.<br />
|-<br />
| [[Pesta D|Pesta Dominik]]||Yale University School of Medicine, CT, US||Dysregulation of muscle ATP synthesis in hypophosphatemic NaPi2a knockout mice and in a patient with hypophosphatemia due to a mutation in NaPi2c. <br />
|- <br />
| [[Picard_M|Picard Martin]]||University of Pennsylvania, PA, US||Quantitative regulation of nuclear gene expression by mitochondrial DNA<br />
heteroplasmy.<br />
|-<br />
| [[Pecina P|Pecina Petr]]||Academy of Sciences of the Czech Republic, Prague, CZ||The use of lymphocytes for diagnostics of mitochondrial disorders.<br />
|-<br />
| [[Purhonen J|Purhonen Janne]]||University Helsinki, FI||<br />
|-<br />
| [[Ristow M|Ristow Michael]]||ETH Zurich, CH||Promoting health and lifespan by increasing mitochondrial ROS formation.<br />
|-<br />
| [[Shirihai O|Shirihai Orian]]||The Shirihai Lab at Boston University, MA, US||<br />
|-<br />
| [[Skulachev VP|Skulachev Vladimir]]||Lomonosov Moscow State University, Moscow, RU||Mitochondria are sources of ROS rather than sinks: Physiological effects of mitochondria-targeted antioxidants.<br />
|-<br />
| [[Starkov AA|Starkov Anatoly]]||Weill Medical College Cornell University, NY, US||<br />
|-<br />
| [[Stiban J|Stiban Johnny]]||Birzeit University, Ramallah, PS||Very long chain ceramides interfere with C16-ceramide-induced channel formation: A plausible mechanism for regulating the initiation of intrinsic apoptosis.<br />
|-<br />
| [[Zorzano A|Zorzano Antonio]]||Universitat de Barcelona, ES||Metabolic implications of mitofusin 2 dysfunction.<br />
|-<br />
| [[Sumbalova Z|Sumbalova Zuzana]]||Comenius University, Bratislava, SK||<br />
|}<br />
<br />
== Sponsors ==<br />
<br />
[[Image:OROBOROS Oxygraph-2k Logo.jpg|left|200px|link=http://www.oroboros.at|OROBOROS]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[Image:HAMLogoRGB.jpg|left|200px|link=http://www.hamilton.ch|Hamilton]]<br />
<br />
<br />
<br />
<br />
<br />
[[Image:Hansatech.jpg|left|200px|link=http://www.hansatech-instruments.com/|Hansatech]]<br />
<br />
== Venue ==<br />
<br />
The symposium will be hosted in the '''University Center in Obergurgl''' 100 km from Innsbruck. The closest airport is in Innsbruck. Participants arriving at Munich airport will take the train or an airport shuttle to Innsbruck. We provide support in arranging the transfer between Munich and Innsbruck. A bus transfer is organized between Innsbruck and Obergurgl.<br />
<br />
The Obergurgl University Center is located at an altitude of 1940 meters above sea-level. The historical buildings, carefully refurbished and equipped with the latest technical systems, can accommodate around 70 persons in 43 bedrooms and includes conference rooms, the Alpine Research Center, laboratory, sports medicine, lounges and the restaurant. All rooms are equipped with an Internet connection. Additional participants are accommodated in smaller guest houses in 5 minutes walking distance. Meals are served for all participants in the University Center. Two conference rooms for a total of 200 persons and three small rooms for round table discussions are available. There is enough space for poster presentations and instrument exhibitions. The cosy bar and lounge area with an open fire place adds to the familiar atmosphere and supports the interactive spirit of the symposium.<br />
<br />
The small village of Obergurgl is situated in an impressive landscape at about 1900 m above sea level in the Ötz valley. It is surrounded by mountains, offering an exceptional view to the Ötz valley glaciers. During summer time the area is famous for hiking and for skiing in winter time. On the doorstep: one of Europe´s finest Biosphere Parks. The unique surroundings offer the possibility of combining scientific discussions while exploring the alpine environment during the famous “walks and talks”. The informal atmosphere of the workshop encourages also students and young post-docs to participate actively in scientific discussions and to develop future collaborations.<br />
<br />
University Centre Obergurgl, Gaisbergweg 5, 6456 Obergurgl, Tyrol, Austria.<br />
Tel.: +43 (0) 512 507 37201 | Fax: +43 (0) 512 507 37400 | Email: obergurgl@uibk.ac.at<br />
<br />
== International programme committee ==<br />
The international programme committee will provide scientific input by active participation, nominate further invited speakers to ensure balanced point/counterpoint introductions, and select the abstracts as a basis for participation (restricted number of participants), for oral 10+5 min presentations, and for flash presentations.<br />
<br />
Chair:<br />
* [[Gnaiger E|Gnaiger Erich]], Medical University Innsbruck, AT. - erich.gnaiger@mitophysiology.org<br />
Members:<br />
* [[Ali SS|Ali Sameh S]], Helmy Institute of Medical Sciences, Giza, Egypt<br />
* [[Borutaite V|Borutaite Vilma]], Lithuanian University of Health Sciences Kaunas, LT<br />
* [[Neurocon|Chakrabarti Sasanka]], Institute of Post-graduate Medical Education & Research, Kolkatta, India<br />
* [[Chinese Society of Mitochondrial Research and Medicine|Chen Quan]], Chinese Academy of Sciences, Nankai University, Beijing <br />
* [[Dela F|Dela Flemming]], University of Copenhagen, DK <br />
* [[Duchen MR|Duchen Michael R]], University College London, UK<br />
* [[Elmer E|Elmér Eskil]], Lund University, SE <br />
* [[Hand SC|Hand Steven]], Louisiana State University, LA, US<br />
* [[Hickey AJ|Hickey Anthony J]], University of Auckland, NZ<br />
* [[Koopman WJ|Koopman Werner J]], Radboud Univ Nijmegen Medical Centre, NL<br />
* [[Tanaka M|Tanaka Masashi]], Toyko Metropolitan Institute of Gerontology, JP<br />
* [[Neufer PD|Neufer P Darrell]], East Carolina University, Greenville, NC, US<br />
* [[Ojuka E|Ojuka Edward]], University of Cape Town, South Africa<br />
* [[Pecina P|Pecina Petr]], Academy of Sciences of the Czech Republic, CZ<br />
* [[Perry CG|Perry Christopher G]], York University, Toronto, Canada<br />
* [[Rossignol R|Rossignol Rodrigue]], Université Victor Segalen-Bordeaux, FR<br />
* [[Vercesi AE|Vercesi Anibal E]], State University of Campinas, Sao Paulo, Brazil<br />
* [[Taiwan Society for Mitochondrial Research and Medicine|Wei Yau-Huei]], Mackay Medical College, New Taipei City, Taiwan<br />
* [[Zorzano A|Zorzano Antonio]], Universitat de Barcelona, Spain<br />
<br />
== Information for invited speakers==<br />
<br />
MiP2014 will be held at the University Centre Obergurgl, with maximum capacity of 124 persons and a price per person per night of € 65.- including a shared double room, breakfast, and dinner. MiP''conferences'' are organized as small meetings facilitating communication between senior and junior participants. In the tradition of MiP''conferences'', we are asking invited speakers to cooperate and try to receive local support for joining us at the prestigious Mitochondrial Physiology Conference (limited to 124 participants). Support will be provided to students and young investigators.<br />
<br />
== Post-conference O2k-Workshop ==<br />
<br />
A [http://www.oroboros.at/?IOC_2014-09_obergurgl post-conference workshop] is organized on the same venue, for hands-on training in mitochondrial respiratory physiology, introducing the combined measurement of high-resolution respirometry and fluorometry (OROBOROS INSTRUMENTS). Since participants come from all-over-the-world, including many students, the combination of the symposium with the workshop makes travelling more economical for the maximum of 24 workshop-participants. The post-conference workshop is financially completely separate from the symposium.<br />
<br />
[[File:MitoGlobal.jpg|left|40px|MitoGlobal]]<br />
== MiP''events'' ==<br />
are listed as '''[[MitoGlobal_Events]]'''.<br />
<br />
<br />
<br />
<br />
<br />
[[Category:MitoPedia]]<br />
[[Category:All]]</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MiP2014&diff=58431MiP20142014-04-15T09:36:39Z<p>Bauer Christiane: /* Venue */</p>
<hr />
<div>[[Image:IUBMB-logo.gif|left|100px|link=http://www.iubmb.org|IUBMB - ''International Union of Biochemistry and Molecular Biology'']]<br />
[[Image:MiPsocietyLOGO.JPG|right|200px|link=http://www.mitophysiology.org|MiPsociety]]<br />
[http://www.mitophysiology.org/?MiP2014 '''<big>MiP2014 - 10<sup>th</sup> MiP''conference'': Joint IUBMB/MiP Symposium on Mitochondrial Physiology - a Point/Counterpoint Meeting</big>''']<br />
<br />
'''''International Union of Biochemistry and Molecular Biology''''' and '''Mitochondrial Physiology Society'''<br />
<br />
Obergurgl, Tyrol, Austria. 2014-Sep-08 to 12<br />
== MiP2014 - a point/counterpoint meeting ==<br />
<br />
Mitochondrial research has recently undergone a dramatic renaissance, due to numerous discoveries revealing its central role in cell death, disease pathology, aging, thermogenesis, oxidative stress, cell signalling, cellular regulation and cell bioenergetics. The aim of the small symposium is to bring together experts in mitochondrial research spanning from the biochemical and molecular level to the interactions within the cell. In addition to the latest scientific findings, new methodological developments in measuring mitochondrial function and dysfunction are presented. A point/counterpoint format is chosen to discuss selected hot topics and some fundamental methodological and conceptual issues.<br />
<br />
The expected attendance is max. 100 participants. ->> Compare [[MiP2013 Programme]]<br />
Abstracts and presentations: refer to [http://www.chem.qmul.ac.uk/iubmb/ IUBMB nomenclature]; >> [[MitoPedia Glossary: Respiratory states|MitoPedia Glossary: '''Respiratory states''']]<br />
<br />
== Programme structure ==<br />
<br />
In each of the 12 sessions a point/counterpoint introduction will be given by invited keynote speakers to provide a balanced overview and promote discussions. Short oral presentations are selected from abstracts, including student presentations. Rapid platform statements will introduce posters which contribute to a specific session. Posters are up throughout the meeting, and poster discussions are integrated into the platform sessions.<br />
<br />
=== A. Mitochondrial respiratory control === <br />
* A1. mtDNA, mt-phenotype and disease – is there biochemical and molecular-genetic evidence to explain observed associations? <br />
* A2. Fission and fusion, mitochondrial structure and function - is there a tight connection? <br />
* A3. Mitochondrial density and normalization of respiration – is there a general biochemical or molecular marker? <br />
* A4. Biochemical reserve capacity, respiratory control ratios and coupling efficiency – are conventional concepts inconsistent?<br />
<br />
=== B. Experimental protocols in mitochondrial biochemistry === <br />
* B1. Incubation media for mt-preparations and experimental temperature in functional studies of mammalian mitochondria – guided by concept or tradition? <br />
* B2. Intracellular oxygen pressure versus oxygen regimes in studies of ROS production and respiration - are mitochondria sources or sinks of ROS?<br />
* B3. Quantitative measurement of mt-membrane potential - potentiometric/fluorometric. <br />
* B4. Instrumental platforms in mitochondrial physiology – industrial presentations.<br />
<br />
=== C. Towards quality assurance === <br />
* C1. Do blood cells versus muscle biopsies provide valid models for diagnosis of mitochondrial pathologies? <br />
* C2. Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
* C3. From availability and quality control of chemicals to mitochondrial respiratory protocols – who controls? <br />
* C4. Standards for the diagnosis of mitochondrial pathologies – a challenge for collaboration with mitochondrial organizations?<br />
Particularly in view of Subtopic C, it may be attractive for mitochondrial organizations to send and support delegates. The conference will, therefore, provide a unique forum for international networking, and may inspire new national groups to be formed in contact with established mitochondrial organizations:<br />
>> '''[[MitoGlobal_Organizations]]'''<br />
<br />
== MiP2014 preliminary participants ==<br />
<br />
in progress<br />
<br />
{| class="wikitable"<br />
|-<br />
! Name !! Institution !! Preliminary title <br />
|-<br />
| [[Ali SS|Ali Sameh S]]||Zewail City for Science and Technology, ET||Oxygen-consuming and ROS-producing activities in synaptosomes.<br />
|-<br />
| [[Bauer CM|Bauer Christiane Maria]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Borutaite_V|Borutaite Vilmante]]||Lithuanian University of Health Sciences, Kaunas, LT||Controversies in testing integrity of mitochondrial membranes.<br />
|-<br />
| [[Brown D|Brown David]]||East Carolina University, NC, US||<br />
|-<br />
| [[Contreras-Ferrat A|Contreras-Ferrat Ariel]]||University of Chile, CL||<br />
|-<br />
| [[Coen P|Coen Paul]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Dohlmann T|Dohlmann Tine]]||University of Copenhagen, DK||<br />
|-<br />
| [[Elmer E|Elmer Eskil]]||Wallenberg Neuroscience Center, Lund, SE||Ex vivo respiratory capacity of human blood cells - a SUITable application of the O2k Oxygraph / A novel pharmacological approach to counteract metabolic crisis.<br />
|-<br />
| [[Felding-Habermann B|Felding-Habermann Brunhilde]]||The Scripps Research Institute, La Jolla, CA, US||Mitochondrial functions in breast cancer progression.<br />
|-<br />
| [[Goodpaster BH|Goodpaster Bret]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Gnaiger E|Gnaiger Erich]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Grefte S|Grefte Sander]]||Radboud University Nijmegen Medical Centre, Nijmegen, NL||Pathophysiology of mitochondrial (dys)function in skeletal muscle.<br />
|-<br />
| [[Hahn D|Hahn Dagmar]]||Inselspital Bern, CH||Validation of Oxygen Consumption measurements in fibroblasts from patients with mitochondrial diseases.<br />
|-<br />
| [[Hand SC|Hand Steven C]]||Louisiana State University, LA, US||Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
|-<br />
| [[Harrison DK|Harrison David K]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Hepple_RT| Hepple Russel T.]]||McGill University, Montreal, CA||Mitochondrial involvement in aging muscle: cause and effect(or).<br />
|-<br />
| [[Hey-Mogensen M|Hey-Mogensen Martin]]||Novo Nordisk A/S Diabetes NBEs & Obesity Biology, Måløv, DK||<br />
|-<br />
| [[Jana Prado F|Jana Prado Fabian]]||University of Chile, CL||<br />
|-<br />
| [[Kane DA| Kane Daniel A.]]||St. Francis Xavier University, Antigonish, CA||Mitochondrial shuttles in muscle.<br />
|-<br />
| [[Kraunsoe R|Kraunsoe Regitze]]||University of Copenhagen, DK||<br />
|-<br />
| [[Koopman WJ|Koopman Werner J]]||Radboud University Nijmegen Medical Centre, NL||Redox regulation of mitochondrial dynamics.<br />
|-<br />
| [[Kowaltowski AJ|Kowaltowski Alicia J.]]||University of Sao Paulo, BR||<br />
|-<br />
| [[Laner V|Laner Verena]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Larsen S|Larsen Steen]]||University of Copenhagen, DK||Normalization of respiration.<br />
|-<br />
| [[Larsson NG|Göran-Larsson Nils]]||Max Planck Institute for the Biology of Ageing, Cologne, DE||Mouse models with mtDNA mutations.<br />
|-<br />
| [[Ojuka E|Ojuka Edward]]||University of Cape Town, ZA||Fission and fusion, mitochondrial structure and function - is there a tight connection?<br />
|-<br />
| [[Perry CG|Perry Christopher G]]||York University, Ontario, CA||Re-visiting critical experimental parameters to control when measuring respiratory kinetics in permeabilized muscle fibres.<br />
|-<br />
| [[Pesta D|Pesta Dominik]]||Yale University School of Medicine, CT, US||Dysregulation of muscle ATP synthesis in hypophosphatemic NaPi2a knockout mice and in a patient with hypophosphatemia due to a mutation in NaPi2c. <br />
|- <br />
| [[Picard_M|Picard Martin]]||University of Pennsylvania, PA, US||Quantitative regulation of nuclear gene expression by mitochondrial DNA<br />
heteroplasmy.<br />
|-<br />
| [[Pecina P|Pecina Petr]]||Academy of Sciences of the Czech Republic, Prague, CZ||The use of lymphocytes for diagnostics of mitochondrial disorders.<br />
|-<br />
| [[Purhonen J|Purhonen Janne]]||University Helsinki, FI||<br />
|-<br />
| [[Ristow M|Ristow Michael]]||ETH Zurich, CH||Promoting health and lifespan by increasing mitochondrial ROS formation.<br />
|-<br />
| [[Shirihai O|Shirihai Orian]]||The Shirihai Lab at Boston University, MA, US||<br />
|-<br />
| [[Skulachev VP|Skulachev Vladimir]]||Lomonosov Moscow State University, Moscow, RU||Mitochondria are sources of ROS rather than sinks: Physiological effects of mitochondria-targeted antioxidants.<br />
|-<br />
| [[Starkov AA|Starkov Anatoly]]||Weill Medical College Cornell University, NY, US||<br />
|-<br />
| [[Stiban J|Stiban Johnny]]||Birzeit University, Ramallah, PS||Very long chain ceramides interfere with C16-ceramide-induced channel formation: A plausible mechanism for regulating the initiation of intrinsic apoptosis.<br />
|-<br />
| [[Zorzano A|Zorzano Antonio]]||Universitat de Barcelona, ES||Metabolic implications of mitofusin 2 dysfunction.<br />
|-<br />
| [[Sumbalova Z|Sumbalova Zuzana]]||Comenius University, Bratislava, SK||<br />
|}<br />
<br />
== Sponsors ==<br />
<br />
[[Image:OROBOROS Oxygraph-2k Logo.jpg|left|200px|link=http://www.oroboros.at|OROBOROS]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[Image:HAMLogoRGB.jpg|left|200px|link=http://www.hamilton.ch|Hamilton]]<br />
<br />
<br />
<br />
<br />
<br />
[[Image:Hansatech.jpg|left|200px|link=http://http://hansatech-instruments.com/|Hansatech]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
== Venue ==<br />
<br />
The symposium will be hosted in the '''University Center in Obergurgl''' 100 km from Innsbruck. The closest airport is in Innsbruck. Participants arriving at Munich airport will take the train or an airport shuttle to Innsbruck. We provide support in arranging the transfer between Munich and Innsbruck. A bus transfer is organized between Innsbruck and Obergurgl.<br />
<br />
The Obergurgl University Center is located at an altitude of 1940 meters above sea-level. The historical buildings, carefully refurbished and equipped with the latest technical systems, can accommodate around 70 persons in 43 bedrooms and includes conference rooms, the Alpine Research Center, laboratory, sports medicine, lounges and the restaurant. All rooms are equipped with an Internet connection. Additional participants are accommodated in smaller guest houses in 5 minutes walking distance. Meals are served for all participants in the University Center. Two conference rooms for a total of 200 persons and three small rooms for round table discussions are available. There is enough space for poster presentations and instrument exhibitions. The cosy bar and lounge area with an open fire place adds to the familiar atmosphere and supports the interactive spirit of the symposium.<br />
<br />
The small village of Obergurgl is situated in an impressive landscape at about 1900 m above sea level in the Ötz valley. It is surrounded by mountains, offering an exceptional view to the Ötz valley glaciers. During summer time the area is famous for hiking and for skiing in winter time. On the doorstep: one of Europe´s finest Biosphere Parks. The unique surroundings offer the possibility of combining scientific discussions while exploring the alpine environment during the famous “walks and talks”. The informal atmosphere of the workshop encourages also students and young post-docs to participate actively in scientific discussions and to develop future collaborations.<br />
<br />
University Centre Obergurgl, Gaisbergweg 5, 6456 Obergurgl, Tyrol, Austria.<br />
Tel.: +43 (0) 512 507 37201 | Fax: +43 (0) 512 507 37400 | Email: obergurgl@uibk.ac.at<br />
<br />
== International programme committee ==<br />
The international programme committee will provide scientific input by active participation, nominate further invited speakers to ensure balanced point/counterpoint introductions, and select the abstracts as a basis for participation (restricted number of participants), for oral 10+5 min presentations, and for flash presentations.<br />
<br />
Chair:<br />
* [[Gnaiger E|Gnaiger Erich]], Medical University Innsbruck, AT. - erich.gnaiger@mitophysiology.org<br />
Members:<br />
* [[Ali SS|Ali Sameh S]], Helmy Institute of Medical Sciences, Giza, Egypt<br />
* [[Borutaite V|Borutaite Vilma]], Lithuanian University of Health Sciences Kaunas, LT<br />
* [[Neurocon|Chakrabarti Sasanka]], Institute of Post-graduate Medical Education & Research, Kolkatta, India<br />
* [[Chinese Society of Mitochondrial Research and Medicine|Chen Quan]], Chinese Academy of Sciences, Nankai University, Beijing <br />
* [[Dela F|Dela Flemming]], University of Copenhagen, DK <br />
* [[Duchen MR|Duchen Michael R]], University College London, UK<br />
* [[Elmer E|Elmér Eskil]], Lund University, SE <br />
* [[Hand SC|Hand Steven]], Louisiana State University, LA, US<br />
* [[Hickey AJ|Hickey Anthony J]], University of Auckland, NZ<br />
* [[Koopman WJ|Koopman Werner J]], Radboud Univ Nijmegen Medical Centre, NL<br />
* [[Tanaka M|Tanaka Masashi]], Toyko Metropolitan Institute of Gerontology, JP<br />
* [[Neufer PD|Neufer P Darrell]], East Carolina University, Greenville, NC, US<br />
* [[Ojuka E|Ojuka Edward]], University of Cape Town, South Africa<br />
* [[Pecina P|Pecina Petr]], Academy of Sciences of the Czech Republic, CZ<br />
* [[Perry CG|Perry Christopher G]], York University, Toronto, Canada<br />
* [[Rossignol R|Rossignol Rodrigue]], Université Victor Segalen-Bordeaux, FR<br />
* [[Vercesi AE|Vercesi Anibal E]], State University of Campinas, Sao Paulo, Brazil<br />
* [[Taiwan Society for Mitochondrial Research and Medicine|Wei Yau-Huei]], Mackay Medical College, New Taipei City, Taiwan<br />
* [[Zorzano A|Zorzano Antonio]], Universitat de Barcelona, Spain<br />
<br />
== Information for invited speakers==<br />
<br />
MiP2014 will be held at the University Centre Obergurgl, with maximum capacity of 124 persons and a price per person per night of € 65.- including a shared double room, breakfast, and dinner. MiP''conferences'' are organized as small meetings facilitating communication between senior and junior participants. In the tradition of MiP''conferences'', we are asking invited speakers to cooperate and try to receive local support for joining us at the prestigious Mitochondrial Physiology Conference (limited to 124 participants). Support will be provided to students and young investigators.<br />
<br />
== Post-conference O2k-Workshop ==<br />
<br />
A [http://www.oroboros.at/?IOC_2014-09_obergurgl post-conference workshop] is organized on the same venue, for hands-on training in mitochondrial respiratory physiology, introducing the combined measurement of high-resolution respirometry and fluorometry (OROBOROS INSTRUMENTS). Since participants come from all-over-the-world, including many students, the combination of the symposium with the workshop makes travelling more economical for the maximum of 24 workshop-participants. The post-conference workshop is financially completely separate from the symposium.<br />
<br />
[[File:MitoGlobal.jpg|left|40px|MitoGlobal]]<br />
== MiP''events'' ==<br />
are listed as '''[[MitoGlobal_Events]]'''.<br />
<br />
<br />
<br />
<br />
<br />
[[Category:MitoPedia]]<br />
[[Category:All]]</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MiP2014&diff=58430MiP20142014-04-15T09:32:32Z<p>Bauer Christiane: /* Sponsors */</p>
<hr />
<div>[[Image:IUBMB-logo.gif|left|100px|link=http://www.iubmb.org|IUBMB - ''International Union of Biochemistry and Molecular Biology'']]<br />
[[Image:MiPsocietyLOGO.JPG|right|200px|link=http://www.mitophysiology.org|MiPsociety]]<br />
[http://www.mitophysiology.org/?MiP2014 '''<big>MiP2014 - 10<sup>th</sup> MiP''conference'': Joint IUBMB/MiP Symposium on Mitochondrial Physiology - a Point/Counterpoint Meeting</big>''']<br />
<br />
'''''International Union of Biochemistry and Molecular Biology''''' and '''Mitochondrial Physiology Society'''<br />
<br />
Obergurgl, Tyrol, Austria. 2014-Sep-08 to 12<br />
== MiP2014 - a point/counterpoint meeting ==<br />
<br />
Mitochondrial research has recently undergone a dramatic renaissance, due to numerous discoveries revealing its central role in cell death, disease pathology, aging, thermogenesis, oxidative stress, cell signalling, cellular regulation and cell bioenergetics. The aim of the small symposium is to bring together experts in mitochondrial research spanning from the biochemical and molecular level to the interactions within the cell. In addition to the latest scientific findings, new methodological developments in measuring mitochondrial function and dysfunction are presented. A point/counterpoint format is chosen to discuss selected hot topics and some fundamental methodological and conceptual issues.<br />
<br />
The expected attendance is max. 100 participants. ->> Compare [[MiP2013 Programme]]<br />
Abstracts and presentations: refer to [http://www.chem.qmul.ac.uk/iubmb/ IUBMB nomenclature]; >> [[MitoPedia Glossary: Respiratory states|MitoPedia Glossary: '''Respiratory states''']]<br />
<br />
== Programme structure ==<br />
<br />
In each of the 12 sessions a point/counterpoint introduction will be given by invited keynote speakers to provide a balanced overview and promote discussions. Short oral presentations are selected from abstracts, including student presentations. Rapid platform statements will introduce posters which contribute to a specific session. Posters are up throughout the meeting, and poster discussions are integrated into the platform sessions.<br />
<br />
=== A. Mitochondrial respiratory control === <br />
* A1. mtDNA, mt-phenotype and disease – is there biochemical and molecular-genetic evidence to explain observed associations? <br />
* A2. Fission and fusion, mitochondrial structure and function - is there a tight connection? <br />
* A3. Mitochondrial density and normalization of respiration – is there a general biochemical or molecular marker? <br />
* A4. Biochemical reserve capacity, respiratory control ratios and coupling efficiency – are conventional concepts inconsistent?<br />
<br />
=== B. Experimental protocols in mitochondrial biochemistry === <br />
* B1. Incubation media for mt-preparations and experimental temperature in functional studies of mammalian mitochondria – guided by concept or tradition? <br />
* B2. Intracellular oxygen pressure versus oxygen regimes in studies of ROS production and respiration - are mitochondria sources or sinks of ROS?<br />
* B3. Quantitative measurement of mt-membrane potential - potentiometric/fluorometric. <br />
* B4. Instrumental platforms in mitochondrial physiology – industrial presentations.<br />
<br />
=== C. Towards quality assurance === <br />
* C1. Do blood cells versus muscle biopsies provide valid models for diagnosis of mitochondrial pathologies? <br />
* C2. Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
* C3. From availability and quality control of chemicals to mitochondrial respiratory protocols – who controls? <br />
* C4. Standards for the diagnosis of mitochondrial pathologies – a challenge for collaboration with mitochondrial organizations?<br />
Particularly in view of Subtopic C, it may be attractive for mitochondrial organizations to send and support delegates. The conference will, therefore, provide a unique forum for international networking, and may inspire new national groups to be formed in contact with established mitochondrial organizations:<br />
>> '''[[MitoGlobal_Organizations]]'''<br />
<br />
== MiP2014 preliminary participants ==<br />
<br />
in progress<br />
<br />
{| class="wikitable"<br />
|-<br />
! Name !! Institution !! Preliminary title <br />
|-<br />
| [[Ali SS|Ali Sameh S]]||Zewail City for Science and Technology, ET||Oxygen-consuming and ROS-producing activities in synaptosomes.<br />
|-<br />
| [[Bauer CM|Bauer Christiane Maria]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Borutaite_V|Borutaite Vilmante]]||Lithuanian University of Health Sciences, Kaunas, LT||Controversies in testing integrity of mitochondrial membranes.<br />
|-<br />
| [[Brown D|Brown David]]||East Carolina University, NC, US||<br />
|-<br />
| [[Contreras-Ferrat A|Contreras-Ferrat Ariel]]||University of Chile, CL||<br />
|-<br />
| [[Coen P|Coen Paul]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Dohlmann T|Dohlmann Tine]]||University of Copenhagen, DK||<br />
|-<br />
| [[Elmer E|Elmer Eskil]]||Wallenberg Neuroscience Center, Lund, SE||Ex vivo respiratory capacity of human blood cells - a SUITable application of the O2k Oxygraph / A novel pharmacological approach to counteract metabolic crisis.<br />
|-<br />
| [[Felding-Habermann B|Felding-Habermann Brunhilde]]||The Scripps Research Institute, La Jolla, CA, US||Mitochondrial functions in breast cancer progression.<br />
|-<br />
| [[Goodpaster BH|Goodpaster Bret]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Gnaiger E|Gnaiger Erich]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Grefte S|Grefte Sander]]||Radboud University Nijmegen Medical Centre, Nijmegen, NL||Pathophysiology of mitochondrial (dys)function in skeletal muscle.<br />
|-<br />
| [[Hahn D|Hahn Dagmar]]||Inselspital Bern, CH||Validation of Oxygen Consumption measurements in fibroblasts from patients with mitochondrial diseases.<br />
|-<br />
| [[Hand SC|Hand Steven C]]||Louisiana State University, LA, US||Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
|-<br />
| [[Harrison DK|Harrison David K]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Hepple_RT| Hepple Russel T.]]||McGill University, Montreal, CA||Mitochondrial involvement in aging muscle: cause and effect(or).<br />
|-<br />
| [[Hey-Mogensen M|Hey-Mogensen Martin]]||Novo Nordisk A/S Diabetes NBEs & Obesity Biology, Måløv, DK||<br />
|-<br />
| [[Jana Prado F|Jana Prado Fabian]]||University of Chile, CL||<br />
|-<br />
| [[Kane DA| Kane Daniel A.]]||St. Francis Xavier University, Antigonish, CA||Mitochondrial shuttles in muscle.<br />
|-<br />
| [[Kraunsoe R|Kraunsoe Regitze]]||University of Copenhagen, DK||<br />
|-<br />
| [[Koopman WJ|Koopman Werner J]]||Radboud University Nijmegen Medical Centre, NL||Redox regulation of mitochondrial dynamics.<br />
|-<br />
| [[Kowaltowski AJ|Kowaltowski Alicia J.]]||University of Sao Paulo, BR||<br />
|-<br />
| [[Laner V|Laner Verena]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Larsen S|Larsen Steen]]||University of Copenhagen, DK||Normalization of respiration.<br />
|-<br />
| [[Larsson NG|Göran-Larsson Nils]]||Max Planck Institute for the Biology of Ageing, Cologne, DE||Mouse models with mtDNA mutations.<br />
|-<br />
| [[Ojuka E|Ojuka Edward]]||University of Cape Town, ZA||Fission and fusion, mitochondrial structure and function - is there a tight connection?<br />
|-<br />
| [[Perry CG|Perry Christopher G]]||York University, Ontario, CA||Re-visiting critical experimental parameters to control when measuring respiratory kinetics in permeabilized muscle fibres.<br />
|-<br />
| [[Pesta D|Pesta Dominik]]||Yale University School of Medicine, CT, US||Dysregulation of muscle ATP synthesis in hypophosphatemic NaPi2a knockout mice and in a patient with hypophosphatemia due to a mutation in NaPi2c. <br />
|- <br />
| [[Picard_M|Picard Martin]]||University of Pennsylvania, PA, US||Quantitative regulation of nuclear gene expression by mitochondrial DNA<br />
heteroplasmy.<br />
|-<br />
| [[Pecina P|Pecina Petr]]||Academy of Sciences of the Czech Republic, Prague, CZ||The use of lymphocytes for diagnostics of mitochondrial disorders.<br />
|-<br />
| [[Purhonen J|Purhonen Janne]]||University Helsinki, FI||<br />
|-<br />
| [[Ristow M|Ristow Michael]]||ETH Zurich, CH||Promoting health and lifespan by increasing mitochondrial ROS formation.<br />
|-<br />
| [[Shirihai O|Shirihai Orian]]||The Shirihai Lab at Boston University, MA, US||<br />
|-<br />
| [[Skulachev VP|Skulachev Vladimir]]||Lomonosov Moscow State University, Moscow, RU||Mitochondria are sources of ROS rather than sinks: Physiological effects of mitochondria-targeted antioxidants.<br />
|-<br />
| [[Starkov AA|Starkov Anatoly]]||Weill Medical College Cornell University, NY, US||<br />
|-<br />
| [[Stiban J|Stiban Johnny]]||Birzeit University, Ramallah, PS||Very long chain ceramides interfere with C16-ceramide-induced channel formation: A plausible mechanism for regulating the initiation of intrinsic apoptosis.<br />
|-<br />
| [[Zorzano A|Zorzano Antonio]]||Universitat de Barcelona, ES||Metabolic implications of mitofusin 2 dysfunction.<br />
|-<br />
| [[Sumbalova Z|Sumbalova Zuzana]]||Comenius University, Bratislava, SK||<br />
|}<br />
<br />
== Sponsors ==<br />
<br />
[[Image:OROBOROS Oxygraph-2k Logo.jpg|left|200px|link=http://www.oroboros.at|OROBOROS]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[Image:HAMLogoRGB.jpg|left|200px|link=http://www.hamilton.ch|Hamilton]]<br />
<br />
<br />
<br />
<br />
<br />
[[Image:Hansatech.jpg|left|200px|link=http://http://hansatech-instruments.com/|Hansatech]]<br />
<br />
== Venue ==<br />
<br />
The symposium will be hosted in the '''University Center in Obergurgl''' 100 km from Innsbruck. The closest airport is in Innsbruck. Participants arriving at Munich airport will take the train or an airport shuttle to Innsbruck. We provide support in arranging the transfer between Munich and Innsbruck. A bus transfer is organized between Innsbruck and Obergurgl.<br />
<br />
The Obergurgl University Center is located at an altitude of 1940 meters above sea-level. The historical buildings, carefully refurbished and equipped with the latest technical systems, can accommodate around 70 persons in 43 bedrooms and includes conference rooms, the Alpine Research Center, laboratory, sports medicine, lounges and the restaurant. All rooms are equipped with an Internet connection. Additional participants are accommodated in smaller guest houses in 5 minutes walking distance. Meals are served for all participants in the University Center. Two conference rooms for a total of 200 persons and three small rooms for round table discussions are available. There is enough space for poster presentations and instrument exhibitions. The cosy bar and lounge area with an open fire place adds to the familiar atmosphere and supports the interactive spirit of the symposium.<br />
<br />
The small village of Obergurgl is situated in an impressive landscape at about 1900 m above sea level in the Ötz valley. It is surrounded by mountains, offering an exceptional view to the Ötz valley glaciers. During summer time the area is famous for hiking and for skiing in winter time. On the doorstep: one of Europe´s finest Biosphere Parks. The unique surroundings offer the possibility of combining scientific discussions while exploring the alpine environment during the famous “walks and talks”. The informal atmosphere of the workshop encourages also students and young post-docs to participate actively in scientific discussions and to develop future collaborations.<br />
<br />
University Centre Obergurgl, Gaisbergweg 5, 6456 Obergurgl, Tyrol, Austria.<br />
Tel.: +43 (0) 512 507 37201 | Fax: +43 (0) 512 507 37400 | Email: obergurgl@uibk.ac.at <br />
<br />
== International programme committee ==<br />
The international programme committee will provide scientific input by active participation, nominate further invited speakers to ensure balanced point/counterpoint introductions, and select the abstracts as a basis for participation (restricted number of participants), for oral 10+5 min presentations, and for flash presentations.<br />
<br />
Chair:<br />
* [[Gnaiger E|Gnaiger Erich]], Medical University Innsbruck, AT. - erich.gnaiger@mitophysiology.org<br />
Members:<br />
* [[Ali SS|Ali Sameh S]], Helmy Institute of Medical Sciences, Giza, Egypt<br />
* [[Borutaite V|Borutaite Vilma]], Lithuanian University of Health Sciences Kaunas, LT<br />
* [[Neurocon|Chakrabarti Sasanka]], Institute of Post-graduate Medical Education & Research, Kolkatta, India<br />
* [[Chinese Society of Mitochondrial Research and Medicine|Chen Quan]], Chinese Academy of Sciences, Nankai University, Beijing <br />
* [[Dela F|Dela Flemming]], University of Copenhagen, DK <br />
* [[Duchen MR|Duchen Michael R]], University College London, UK<br />
* [[Elmer E|Elmér Eskil]], Lund University, SE <br />
* [[Hand SC|Hand Steven]], Louisiana State University, LA, US<br />
* [[Hickey AJ|Hickey Anthony J]], University of Auckland, NZ<br />
* [[Koopman WJ|Koopman Werner J]], Radboud Univ Nijmegen Medical Centre, NL<br />
* [[Tanaka M|Tanaka Masashi]], Toyko Metropolitan Institute of Gerontology, JP<br />
* [[Neufer PD|Neufer P Darrell]], East Carolina University, Greenville, NC, US<br />
* [[Ojuka E|Ojuka Edward]], University of Cape Town, South Africa<br />
* [[Pecina P|Pecina Petr]], Academy of Sciences of the Czech Republic, CZ<br />
* [[Perry CG|Perry Christopher G]], York University, Toronto, Canada<br />
* [[Rossignol R|Rossignol Rodrigue]], Université Victor Segalen-Bordeaux, FR<br />
* [[Vercesi AE|Vercesi Anibal E]], State University of Campinas, Sao Paulo, Brazil<br />
* [[Taiwan Society for Mitochondrial Research and Medicine|Wei Yau-Huei]], Mackay Medical College, New Taipei City, Taiwan<br />
* [[Zorzano A|Zorzano Antonio]], Universitat de Barcelona, Spain<br />
<br />
== Information for invited speakers==<br />
<br />
MiP2014 will be held at the University Centre Obergurgl, with maximum capacity of 124 persons and a price per person per night of € 65.- including a shared double room, breakfast, and dinner. MiP''conferences'' are organized as small meetings facilitating communication between senior and junior participants. In the tradition of MiP''conferences'', we are asking invited speakers to cooperate and try to receive local support for joining us at the prestigious Mitochondrial Physiology Conference (limited to 124 participants). Support will be provided to students and young investigators.<br />
<br />
== Post-conference O2k-Workshop ==<br />
<br />
A [http://www.oroboros.at/?IOC_2014-09_obergurgl post-conference workshop] is organized on the same venue, for hands-on training in mitochondrial respiratory physiology, introducing the combined measurement of high-resolution respirometry and fluorometry (OROBOROS INSTRUMENTS). Since participants come from all-over-the-world, including many students, the combination of the symposium with the workshop makes travelling more economical for the maximum of 24 workshop-participants. The post-conference workshop is financially completely separate from the symposium.<br />
<br />
[[File:MitoGlobal.jpg|left|40px|MitoGlobal]]<br />
== MiP''events'' ==<br />
are listed as '''[[MitoGlobal_Events]]'''.<br />
<br />
<br />
<br />
<br />
<br />
[[Category:MitoPedia]]<br />
[[Category:All]]</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=File:Hansatech.jpg&diff=58429File:Hansatech.jpg2014-04-15T09:30:51Z<p>Bauer Christiane: </p>
<hr />
<div></div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MiP2014&diff=58388MiP20142014-04-14T17:20:24Z<p>Bauer Christiane: /* MiP2014 preliminary participants */</p>
<hr />
<div>[[Image:IUBMB-logo.gif|left|100px|link=http://www.iubmb.org|IUBMB - ''International Union of Biochemistry and Molecular Biology'']]<br />
[[Image:MiPsocietyLOGO.JPG|right|200px|link=http://www.mitophysiology.org|MiPsociety]]<br />
[http://www.mitophysiology.org/?MiP2014 '''<big>MiP2014 - 10<sup>th</sup> MiP''conference'': Joint IUBMB/MiP Symposium on Mitochondrial Physiology - a Point/Counterpoint Meeting</big>''']<br />
<br />
'''''International Union of Biochemistry and Molecular Biology''''' and '''Mitochondrial Physiology Society'''<br />
<br />
Obergurgl, Tyrol, Austria. 2014-Sep-08 to 12<br />
== MiP2014 - a point/counterpoint meeting ==<br />
<br />
Mitochondrial research has recently undergone a dramatic renaissance, due to numerous discoveries revealing its central role in cell death, disease pathology, aging, thermogenesis, oxidative stress, cell signalling, cellular regulation and cell bioenergetics. The aim of the small symposium is to bring together experts in mitochondrial research spanning from the biochemical and molecular level to the interactions within the cell. In addition to the latest scientific findings, new methodological developments in measuring mitochondrial function and dysfunction are presented. A point/counterpoint format is chosen to discuss selected hot topics and some fundamental methodological and conceptual issues.<br />
<br />
The expected attendance is max. 100 participants. ->> Compare [[MiP2013 Programme]]<br />
Abstracts and presentations: refer to [http://www.chem.qmul.ac.uk/iubmb/ IUBMB nomenclature]; >> [[MitoPedia Glossary: Respiratory states|MitoPedia Glossary: '''Respiratory states''']]<br />
<br />
== Programme structure ==<br />
<br />
In each of the 12 sessions a point/counterpoint introduction will be given by invited keynote speakers to provide a balanced overview and promote discussions. Short oral presentations are selected from abstracts, including student presentations. Rapid platform statements will introduce posters which contribute to a specific session. Posters are up throughout the meeting, and poster discussions are integrated into the platform sessions.<br />
<br />
=== A. Mitochondrial respiratory control === <br />
* A1. mtDNA, mt-phenotype and disease – is there biochemical and molecular-genetic evidence to explain observed associations? <br />
* A2. Fission and fusion, mitochondrial structure and function - is there a tight connection? <br />
* A3. Mitochondrial density and normalization of respiration – is there a general biochemical or molecular marker? <br />
* A4. Biochemical reserve capacity, respiratory control ratios and coupling efficiency – are conventional concepts inconsistent?<br />
<br />
=== B. Experimental protocols in mitochondrial biochemistry === <br />
* B1. Incubation media for mt-preparations and experimental temperature in functional studies of mammalian mitochondria – guided by concept or tradition? <br />
* B2. Intracellular oxygen pressure versus oxygen regimes in studies of ROS production and respiration - are mitochondria sources or sinks of ROS?<br />
* B3. Quantitative measurement of mt-membrane potential - potentiometric/fluorometric. <br />
* B4. Instrumental platforms in mitochondrial physiology – industrial presentations.<br />
<br />
=== C. Towards quality assurance === <br />
* C1. Do blood cells versus muscle biopsies provide valid models for diagnosis of mitochondrial pathologies? <br />
* C2. Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
* C3. From availability and quality control of chemicals to mitochondrial respiratory protocols – who controls? <br />
* C4. Standards for the diagnosis of mitochondrial pathologies – a challenge for collaboration with mitochondrial organizations?<br />
Particularly in view of Subtopic C, it may be attractive for mitochondrial organizations to send and support delegates. The conference will, therefore, provide a unique forum for international networking, and may inspire new national groups to be formed in contact with established mitochondrial organizations:<br />
>> '''[[MitoGlobal_Organizations]]'''<br />
<br />
== MiP2014 preliminary participants ==<br />
<br />
in progress<br />
<br />
{| class="wikitable"<br />
|-<br />
! Name !! Institution !! Preliminary title <br />
|-<br />
| [[Ali SS|Ali Sameh S]]||Zewail City for Science and Technology, ET||Oxygen-consuming and ROS-producing activities in synaptosomes.<br />
|-<br />
| [[Bauer CM|Bauer Christiane Maria]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Borutaite_V|Borutaite Vilmante]]||Lithuanian University of Health Sciences, Kaunas, LT||Controversies in testing integrity of mitochondrial membranes.<br />
|-<br />
| [[Brown D|Brown David]]||East Carolina University, NC, US||<br />
|-<br />
| [[Contreras-Ferrat A|Contreras-Ferrat Ariel]]||University of Chile, CL||<br />
|-<br />
| [[Coen P|Coen Paul]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Dohlmann T|Dohlmann Tine]]||University of Copenhagen, DK||<br />
|-<br />
| [[Elmer E|Elmer Eskil]]||Wallenberg Neuroscience Center, Lund, SE||Ex vivo respiratory capacity of human blood cells - a SUITable application of the O2k Oxygraph / A novel pharmacological approach to counteract metabolic crisis.<br />
|-<br />
| [[Felding-Habermann B|Felding-Habermann Brunhilde]]||The Scripps Research Institute, La Jolla, CA, US||Mitochondrial functions in breast cancer progression.<br />
|-<br />
| [[Goodpaster BH|Goodpaster Bret]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Gnaiger E|Gnaiger Erich]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Grefte S|Grefte Sander]]||Radboud University Nijmegen Medical Centre, Nijmegen, NL||Pathophysiology of mitochondrial (dys)function in skeletal muscle.<br />
|-<br />
| [[Hahn D|Hahn Dagmar]]||Inselspital Bern, CH||Validation of Oxygen Consumption measurements in fibroblasts from patients with mitochondrial diseases.<br />
|-<br />
| [[Hand SC|Hand Steven C]]||Louisiana State University, LA, US||Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
|-<br />
| [[Harrison DK|Harrison David K]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Hepple_RT| Hepple Russel T.]]||McGill University, Montreal, CA||Mitochondrial involvement in aging muscle: cause and effect(or).<br />
|-<br />
| [[Hey-Mogensen M|Hey-Mogensen Martin]]||Novo Nordisk A/S Diabetes NBEs & Obesity Biology, Måløv, DK||<br />
|-<br />
| [[Jana Prado F|Jana Prado Fabian]]||University of Chile, CL||<br />
|-<br />
| [[Kane DA| Kane Daniel A.]]||St. Francis Xavier University, Antigonish, CA||Mitochondrial shuttles in muscle.<br />
|-<br />
| [[Kraunsoe R|Kraunsoe Regitze]]||University of Copenhagen, DK||<br />
|-<br />
| [[Koopman WJ|Koopman Werner J]]||Radboud University Nijmegen Medical Centre, NL||Redox regulation of mitochondrial dynamics.<br />
|-<br />
| [[Kowaltowski AJ|Kowaltowski Alicia J.]]||University of Sao Paulo, BR||<br />
|-<br />
| [[Laner V|Laner Verena]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Larsen S|Larsen Steen]]||University of Copenhagen, DK||Normalization of respiration.<br />
|-<br />
| [[Larsson NG|Göran-Larsson Nils]]||Max Planck Institute for the Biology of Ageing, Cologne, DE||Mouse models with mtDNA mutations.<br />
|-<br />
| [[Ojuka E|Ojuka Edward]]||University of Cape Town, ZA||Fission and fusion, mitochondrial structure and function - is there a tight connection?<br />
|-<br />
| [[Perry CG|Perry Christopher G]]||York University, Ontario, CA||Re-visiting critical experimental parameters to control when measuring respiratory kinetics in permeabilized muscle fibres.<br />
|-<br />
| [[Pesta D|Pesta Dominik]]||Yale University School of Medicine, CT, US||Dysregulation of muscle ATP synthesis in hypophosphatemic NaPi2a knockout mice and in a patient with hypophosphatemia due to a mutation in NaPi2c. <br />
|- <br />
| [[Picard_M|Picard Martin]]||University of Pennsylvania, PA, US||Quantitative regulation of nuclear gene expression by mitochondrial DNA<br />
heteroplasmy.<br />
|-<br />
| [[Pecina P|Pecina Petr]]||Academy of Sciences of the Czech Republic, Prague, CZ||The use of lymphocytes for diagnostics of mitochondrial disorders.<br />
|-<br />
| [[Purhonen J|Purhonen Janne]]||University Helsinki, FI||<br />
|-<br />
| [[Ristow M|Ristow Michael]]||ETH Zurich, CH||Promoting health and lifespan by increasing mitochondrial ROS formation.<br />
|-<br />
| [[Shirihai O|Shirihai Orian]]||The Shirihai Lab at Boston University, MA, US||<br />
|-<br />
| [[Skulachev VP|Skulachev Vladimir]]||Lomonosov Moscow State University, Moscow, RU||Mitochondria are sources of ROS rather than sinks: Physiological effects of mitochondria-targeted antioxidants.<br />
|-<br />
| [[Starkov AA|Starkov Anatoly]]||Weill Medical College Cornell University, NY, US||<br />
|-<br />
| [[Stiban J|Stiban Johnny]]||Birzeit University, Ramallah, PS||Very long chain ceramides interfere with C16-ceramide-induced channel formation: A plausible mechanism for regulating the initiation of intrinsic apoptosis.<br />
|-<br />
| [[Zorzano A|Zorzano Antonio]]||Universitat de Barcelona, ES||Metabolic implications of mitofusin 2 dysfunction.<br />
|-<br />
| [[Sumbalova Z|Sumbalova Zuzana]]||Comenius University, Bratislava, SK||<br />
|}<br />
<br />
== Sponsors ==<br />
<br />
[[Image:OROBOROS Oxygraph-2k Logo.jpg|left|200px|link=http://www.oroboros.at|OROBOROS]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[Image:HAMLogoRGB.jpg|left|200px|link=http://www.hamilton.ch|Hamilton]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
== Venue ==<br />
<br />
The symposium will be hosted in the '''University Center in Obergurgl''' 100 km from Innsbruck. The closest airport is in Innsbruck. Participants arriving at Munich airport will take the train or an airport shuttle to Innsbruck. We provide support in arranging the transfer between Munich and Innsbruck. A bus transfer is organized between Innsbruck and Obergurgl.<br />
<br />
The Obergurgl University Center is located at an altitude of 1940 meters above sea-level. The historical buildings, carefully refurbished and equipped with the latest technical systems, can accommodate around 70 persons in 43 bedrooms and includes conference rooms, the Alpine Research Center, laboratory, sports medicine, lounges and the restaurant. All rooms are equipped with an Internet connection. Additional participants are accommodated in smaller guest houses in 5 minutes walking distance. Meals are served for all participants in the University Center. Two conference rooms for a total of 200 persons and three small rooms for round table discussions are available. There is enough space for poster presentations and instrument exhibitions. The cosy bar and lounge area with an open fire place adds to the familiar atmosphere and supports the interactive spirit of the symposium.<br />
<br />
The small village of Obergurgl is situated in an impressive landscape at about 1900 m above sea level in the Ötz valley. It is surrounded by mountains, offering an exceptional view to the Ötz valley glaciers. During summer time the area is famous for hiking and for skiing in winter time. On the doorstep: one of Europe´s finest Biosphere Parks. The unique surroundings offer the possibility of combining scientific discussions while exploring the alpine environment during the famous “walks and talks”. The informal atmosphere of the workshop encourages also students and young post-docs to participate actively in scientific discussions and to develop future collaborations.<br />
<br />
University Centre Obergurgl, Gaisbergweg 5, 6456 Obergurgl, Tyrol, Austria.<br />
Tel.: +43 (0) 512 507 37201 | Fax: +43 (0) 512 507 37400 | Email: obergurgl@uibk.ac.at <br />
<br />
== International programme committee ==<br />
The international programme committee will provide scientific input by active participation, nominate further invited speakers to ensure balanced point/counterpoint introductions, and select the abstracts as a basis for participation (restricted number of participants), for oral 10+5 min presentations, and for flash presentations.<br />
<br />
Chair:<br />
* [[Gnaiger E|Gnaiger Erich]], Medical University Innsbruck, AT. - erich.gnaiger@mitophysiology.org<br />
Members:<br />
* [[Ali SS|Ali Sameh S]], Helmy Institute of Medical Sciences, Giza, Egypt<br />
* [[Borutaite V|Borutaite Vilma]], Lithuanian University of Health Sciences Kaunas, LT<br />
* [[Neurocon|Chakrabarti Sasanka]], Institute of Post-graduate Medical Education & Research, Kolkatta, India<br />
* [[Chinese Society of Mitochondrial Research and Medicine|Chen Quan]], Chinese Academy of Sciences, Nankai University, Beijing <br />
* [[Dela F|Dela Flemming]], University of Copenhagen, DK <br />
* [[Duchen MR|Duchen Michael R]], University College London, UK<br />
* [[Elmer E|Elmér Eskil]], Lund University, SE <br />
* [[Hand SC|Hand Steven]], Louisiana State University, LA, US<br />
* [[Hickey AJ|Hickey Anthony J]], University of Auckland, NZ<br />
* [[Koopman WJ|Koopman Werner J]], Radboud Univ Nijmegen Medical Centre, NL<br />
* [[Tanaka M|Tanaka Masashi]], Toyko Metropolitan Institute of Gerontology, JP<br />
* [[Neufer PD|Neufer P Darrell]], East Carolina University, Greenville, NC, US<br />
* [[Ojuka E|Ojuka Edward]], University of Cape Town, South Africa<br />
* [[Pecina P|Pecina Petr]], Academy of Sciences of the Czech Republic, CZ<br />
* [[Perry CG|Perry Christopher G]], York University, Toronto, Canada<br />
* [[Rossignol R|Rossignol Rodrigue]], Université Victor Segalen-Bordeaux, FR<br />
* [[Vercesi AE|Vercesi Anibal E]], State University of Campinas, Sao Paulo, Brazil<br />
* [[Taiwan Society for Mitochondrial Research and Medicine|Wei Yau-Huei]], Mackay Medical College, New Taipei City, Taiwan<br />
* [[Zorzano A|Zorzano Antonio]], Universitat de Barcelona, Spain<br />
<br />
== Information for invited speakers==<br />
<br />
MiP2014 will be held at the University Centre Obergurgl, with maximum capacity of 124 persons and a price per person per night of € 65.- including a shared double room, breakfast, and dinner. MiP''conferences'' are organized as small meetings facilitating communication between senior and junior participants. In the tradition of MiP''conferences'', we are asking invited speakers to cooperate and try to receive local support for joining us at the prestigious Mitochondrial Physiology Conference (limited to 124 participants). Support will be provided to students and young investigators.<br />
<br />
== Post-conference O2k-Workshop ==<br />
<br />
A [http://www.oroboros.at/?IOC_2014-09_obergurgl post-conference workshop] is organized on the same venue, for hands-on training in mitochondrial respiratory physiology, introducing the combined measurement of high-resolution respirometry and fluorometry (OROBOROS INSTRUMENTS). Since participants come from all-over-the-world, including many students, the combination of the symposium with the workshop makes travelling more economical for the maximum of 24 workshop-participants. The post-conference workshop is financially completely separate from the symposium.<br />
<br />
[[File:MitoGlobal.jpg|left|40px|MitoGlobal]]<br />
== MiP''events'' ==<br />
are listed as '''[[MitoGlobal_Events]]'''.<br />
<br />
<br />
<br />
<br />
<br />
[[Category:MitoPedia]]<br />
[[Category:All]]</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MitoCom_Events&diff=58240MitoCom Events2014-04-01T08:28:15Z<p>Bauer Christiane: </p>
<hr />
<div>{{MitoCom}}<br />
<br />
<br />
== ''MitoCom'' News ==<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
__TOC__<br />
<br />
== ''MitoCom'' Track Record 2014 ==<br />
<br />
=== Iditarod Alaska 2014 === <br />
<br />
Dr. Erich Gnaiger (Medical University of Innsbruck and OROBOROS INSTRUMENTS) and his collaborator Verena Laner (OROBOROS INSTRUMENTS) joined the project of [[Davis MS|Michael Davis]] (Oklahoma State University) in Big Lake, Alaska. Cumulative training effects on the mitochondrial function of alaskan sled dogs were studied at Happy Trails Kennel in Big Lake, Alaska. Further participants were [[Boushel RC|Robert Boushel]] (Institute for Sport and Health Sciences, Stockholm, SE) as well as Benjamin F. Miller and Karyn Hamilton (Colorado State University, Fort Collins, US). [http://www.oroboros.at/index.php?id=racing-dogs_alaska More info]<br />
<br />
=== O2k-Workshop on HRR and O2k-Fluorometry during [http://www.ntnu.edu/isb/enjoy-the-cold 'Enjoy the Cold'] in Spitsbergen, NO, March 27===<br />
Dr. Erich Gnaiger gave a presentation entitled ,Mitochondrial respiratory function in the cold‘ on March 26.<br />
<br />
<br />
== ''MitoCom'' Track Record 2013 ==<br />
<br />
=== O2k-Workshop, Mumbai, India, January 31 ===<br />
<br />
<br />
<br />
=== O2k-Workshop, Mahalabaleshwar, India, January 27-29 ===<br />
Lectures on HRR and Fluorometry were given by Dr. Erich Gnaiger during the 38th Seminar in Mahalabaleshwar.<br />
<br />
<br />
=== O2k-Workshop, Bangalore, India, Dec 18 prior SMRM2013 ===<br />
A one-day O2k-Workshop on HRR and Fluorometry was given by Dr. Erich Gnaiger.<br />
<br />
<br />
=== O2k-Fluorometry Workshop, Trondheim, Norway, Dec 10-11 ===<br />
* [[MiPNet18.11_IOC82|IOC82]]<br />
Dr. Erich Gnaiger and Verena Laner gave an O2k-Workshop, focused on the Fluorescence LED-2 Module prior the seminar 'Exercise in Medicine', which was organized by [http://www.ntnu.no/cerg CERG] of the NTNU University.<br />
<br />
<br />
=== O2k-Luncheon Seminar, Seoul, Korea, Nov 05 @ ASMRM2013 ===<br />
* IOC81<br />
Dr. Erich Gnaiger (Medical University of Innsbruck) followed the invitation of Prof. Hong Kyu Lee to give a talk at the ASMRM2013 meeting. Furthermore he took this opportunity to give an O2k-Luncheon Workshop on HRR and Fluorometry.<br />
<br />
<br />
=== O2k-Fluorometry Workshop, Schroecken, Austria, Oct 09 - 14 ===<br />
* [[MiPNet18.09_IOC80|IOC80]]<br />
A new concept, integrating the Flurometry-Workshop into the Basic-O2k-Workshop, was celebrated at the 80th O2k-Workshop in Schroecken. Tutors: Dr. Erich Gnaiger, Mona Fontana-Ayoub, Verena Laner and Gerhard Krumschnabel.<br />
<br />
<br />
=== O2k-Fluorometry Workshop, Obergurgl, Austria, September 19-23 ===<br />
* [[MiPNet18.07_IOC79|IOC79]]<br />
Prior MiP2013, a O2k-Fluorometry Workshop was given in Obergurgl by Dr. Erich Gnaiger, Dr. Mario Fasching, Mona Fontana-Ayoub and Verena Laner. Hands-on sessions for ~30 participants on 6 O2ks.<br />
<br />
<br />
=== O2k-Workshop @ MiPsummer, Copenhagen, DK, August 27-29 ===<br />
* IOC78 - Tutors: Dr. Erich Gnaiger and Verena Laner<br />
<br />
=== O2k-Luncheon Seminar @ UMDF, Newport Beach, CA, US, June 13 ===<br />
* IOC77<br />
<br />
<br />
<br />
=== Prof. Gottfried Schatz === <br />
<br />
[https://www.i-med.ac.at/mypoint/thema/675045.html Article of the Medical University of Innsbruck]<br />
<br />
=== O2k-Workshop, Prague, Austria, May 28 - 29===<br />
* [[MiPNet18.04_IOC76|IOC76]]<br />
<br />
<br />
=== O2k-Workshop, Schroecken, Austria, Apr 09 - 14 ===<br />
* [[MiPNet18.03_IOC75|IOC75]]<br />
<br />
<br />
=== Xtreme Everst 2 - high-altitude research in Nepal===<br />
<br />
<br />
<br />
[[File: Erich_EBC.jpg|right|200px|Erich Gnaiger with 3 Oxygraphs at the Everest Base Camp]] <br />
<br />
[[File: KTM_Andrew_Verena.jpg|right|200px|Verena Laner assisted Andrew Murray in the Kathamandu Laboratory]] <br />
<br />
Dr. Erich Gnaiger and Verena Laner participated at the Xtreme Everest 2 Expedition in Nepal.<br />
<br />
'''Erich Gnaiger''' (Innsbruck, AT) joined the Xtreme Everest 2 mitochondrial team at Everest Base Camp (5,300 m) where acclimation to high altitude was studied using three O2k (March to May 2013). In Kathmandu (Nepal) at low altitude (1,400 m), Andrew Murray (Cambridge, UK) operated high-resolution respirometry on human musle biopsies with assistance by '''Verena Laner''' (Innsbruck, AT).<br />
<br />
<br />
More infomation: [http://www.oroboros.at/?Xtreme-Everest2 Xtreme Everest 2]<br />
<br />
<br />
Some impressions of the Expedition:<br />
<br />
* [http://www.oroboros.at/?Everest-Base-Camp Everest Base Camp]<br />
* [http://www.oroboros.at/?KTM Kathmandu Laboratory]<br />
<br />
<br />
<br />
The participants from Innsbruck are supported by the K-Regio project ''MitoCom Tyrol'', D. Swarovski Research Laboratory (Medical University of Innsbruck), and OROBOROS INSTRUMENTS.<br />
<br />
<br />
<br />
=== O2k-TPP-Workshop, Innsbruck, Austria, Jan 22 - 25===<br />
* [[MiPNet18.02_IOC74|IOC74]]<br />
<br />
<br />
=== O2k-Workshop, Kolkata, India, January 15 - 16===<br />
*[[MiPNet18.01_IOC73|IOC73]]<br />
<br />
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== ''MitoCom'' Track Record 2012 ==<br />
<br />
<br />
=== O2k-Workshop, Schroecken, Austria, Dec 5 - 10 ===<br />
* [[IOC72]]<br />
<br />
<br />
=== Bioblast 2012 - Conference on Mitochondrial Competence===<br />
<br />
The Bioblast Conference 2012 took place in Hotel Grauer Bär from 10<sup>th</sup> to 12<sup>th</sup> December 2012.<br />
With more than 70 participants from 19 nations the conference was a great success.<br />
<br />
For the article of the Medical University Innsbruck, see: [https://www.i-med.ac.at/mypoint/news/668966.html MUI website]<br />
<br />
More information: [[Bioblast 2012]]<br />
<br />
<br />
<br />
=== Padua Muscle Days, Nov 23 - 25===<br />
<br />
After the satellite meeting in Vienna on October 17th, Prof. Dr. Gnaiger was invited by Prof. Dr. Ugo Carraro (University of Padua) to Padua for a talk on high-resolution respirometry in permeabilized fibers from small biopsies of human muscle.<br />
<br />
<br />
=== MCI Forum Technologie & Life Sciences, Innsbruck, Nov 6===<br />
<br />
In her talk, Mag. Verena Laner will outline the development of the company OROBOROS INSTRUMENTS and will walk you through the early company days up to the current K-Regio Project ''MitoCom Tyrol''. [http://www.mci.edu/en/home/dokumentenverwaltung/category/164-pressemitteilung-20121115?download=3866%3Aprogrammflyer-forum-biotechnologie Programme]<br />
<br />
<br />
=== Satellite Meeting Vienna, Oct 17 ===<br />
<br />
Dr. Erich Gnaiger participated at the Satellite Meeting in Vienna, which took place on 17th October.<br />
<br />
Attendees of this meeting were Delegates of the University in Padua (Univ.-Prof. Dr. Ugo Carraro and Dr. Sandra Zampieri), University in Chieti (Univ.-Prof. Dr. Protasi) and University in Rome (Univ.-Prof. Dr. Andrea Musaro).<br />
<br />
The meeting was organized by Dr. Kern from the Ludwig Boltzmann Institute in Vienna.<br />
<br />
<br />
=== Standort Lunch Vienna, 2012 Sep 27 ===<br />
<br />
Dr. Erich Gnaiger presented the ''MitoCom'' project at the Standort Lunch in Vienna, organized by the Standortagentur Tirol. [http://www.standort-tirol.at/page.cfm?vpath=veranstaltungen/details&genericpageid=35834 More information]. <br />
<br />
The article of the STANDORT Tirol you can find in [http://www.standort-tirol.at/data.cfm?vpath=der_standort/standort-tirol_04-2012_final issue no. 15, page 8]<br />
<br />
<br />
=== Medizintechnikforum Hall, 2012 Sep 20 to 21 ===<br />
<br />
OROBOROS INSTRUMENTS exhibited at the [http://www.jahrestagung-oegbmt-2012.at/uploads/Programm_OEGBMT_Final.pdf Medizintechnikforum Hall], showing latest developments in high-resolution respirometry in combination with the O2k-Fluorescence LED2-Module. ''MitoCom Tyrol'' was presented together with the Open Innovation project, both supported by the Government of Tyrol.<br />
<br />
<br />
=== Tiroltag, Europäisches Forum Alpbach, 2012 Aug 19 ===<br />
<br />
Verena Laner, the project manager of MitoCom Tyrol, took part at the Tirolday, which was the "Opening" of the European Congress in Alpbach 2012. The main topic was "The future of sports in the Alps", which is an integral part of the ''MitoCom'' project. Additionally to the presentations and the award presentation ceremony for young scientists, there was a podium discussion with well-known experts such as the famous alpinist Reinhard Messner.<br />
<br />
<br />
===''MitoCom'' Cooperation AltitudeOmics 2012, 2012 July 20 to Aug 13 ===<br />
<br />
Dr. Erich Gnaiger participated in the [http://www.oroboros.at/?Bolivia2012 AltitudeOmics2012] project in Bolivia and provided the scientists with two O2ks and his expertise. Aim of the study was to investigate the impacts of hypoxia/acclimatization onto molecular mechanisms of the human cells.<br />
<br />
<br />
[[File:BabyJoseph Visit Innsbruck.JPG|right|350px|Prof. Dr. Fr. Baby Joseph und Prof. Dr. Erich Gnaiger]] <br />
<br />
=== Visiting scientist: Prof. Dr. Fr. Baby Joseph, July 19 ===<br />
<br />
On July 19th, Prof. Dr. Fr. Baby Joseph visited the OROBOROS office.<br />
<br />
[[Research_link_to_Prof_Baby_Joseph_India|Time schedule of the research visit]]<br />
<br />
=== Houska Award 2011 - First price for ''MitoCom Tyrol'', April 26 ===<br />
* First price - Medical University of Innsbruck: http://www.oroboros.at/?Houska-Award_2011<br />
* http://www.houskapreis.at/siegerprojekt-2011-licht-in-die-kraftwerke-der-zellen<br />
* http://www.houskapreis.at<br />
* http://www.tt.com/Nachrichten/4702341-6/houska-preise-an-universit%C3%A4ten-in-innsbruck-und-graz.csp<br />
<br />
=== Mitolab O2k-Fluorometry Workshop Madrid, Spain, May 7 ===<br />
* [http://vmbacterio.cbm.uam.es/mitolab/Programa.aspx?Opcion=Mitolab Mitolab Workshop] by [[Gnaiger E|Erich Gnaiger]]<br />
* Contact: Eduardo Rial (Email: rial@cib.csic.es) and [[Cadenas S|Susana Cadenas]] (Email: susana.cadenas@salud.madrid.org)<br />
<br />
>> See also [[MitoCom_Tyrol - Report]] '''<br />
<br />
=== O2k-Workshop Schröcken, Austria, April 11 to 16 ===<br />
* [[IOC68]]<br />
<br />
=== O2k-Workshop Sydney, Australia, April 2 to 4 ===<br />
* [http://www.oroboros.at/?IO67 '''IOC67''' - an International O2k-Workshop of ''MitoCom Tyrol''].<br />
<br />
=== First O2k-Fluorometry Workshop, Innsbruck, March 15 to 16 ===<br />
* [http://www.oroboros.at/?IOC66 IOC66 O2k-Fluorometry Workshop]. An International O2k-Workshop of ''MitoCom Tyrol''.<br />
<br />
=== Manuscript accepted for publication in PloS One, March 9 ===<br />
* "'''[[Votion 2012 PLoS One|Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle]]'''" (by Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D) was accepted for publication in ''PloS One''. <br />
* From the anonymous referee's comments: <br />
** Reviewer #1: "This is an original work performed with state of the art methodologies, as high-resolution respirometry. The results indicate that not only mitochondrial capacity is a limiting factor of horse performance but also the modalities of energy substrate utilization. Horse is an animal of choice for energetic studies since it shows a huge consumption of oxygen during muscle contraction, suggesting an positive adaptation of the mitochondrial system as compared to other animals. .. This article provides new findings on the link between mitochondrial capacity and whole animal body size and aerobic capacity. It also clarifies whether mitochondrial capacity is a controling factor for horse performance. An important finding is also that the control by the phosphorylation system is stable and does not vary with the performance of the horse. In contrast, the relative utilization of complex I versus complex II varies witht he performance, and this is a new finding which adds up to our knowledge of energy regulation. This work is of broad interest for sport physiology, metabolic researchers and aging research."<br />
** Reviewer #2: "The results could be described in a much easier way if utilizing classical definitions of mitochondrial respiratory parameters (from the late Britton Chance's milestones), such as state IV (or II), state III, uncoupled respiration, respiratory control ratio and so on." (''Comment:'' [[Glossary: Respiratory states]])<br />
<br />
<br />
== ''MitoCom'' Track Record 2011 ==<br />
<br />
* 2011-12-10 to 15: [http://www.oroboros.at/index.php?nextcourse#c3124 Internatl. O2k-Workshop on High-Resolution Respirometry, Schröcken, Austria]<br />
<br />
* 2011-12-08: [http://www.oroboros.at/index.php?nextcourse#c3202 O2k-Workshop on High-Resolution Respirometry, Hyderabad, India]<br />
<br />
<br />
* See also: [http://www.oroboros.at/index.php?calendar Science Calendar]<br />
<br />
=== 2011-11-29: 9. Tiroler Innovationstag ===<br />
<br />
* 13:00-14:30 [http://www.standort-tirol.at/page.cfm?vpath=veranstaltungen/details&genericpageid=29311 Cluster-Partnertreffen LIFE SCIENCES Tirol]<br />
<br />
:'''Programm'''<br />
<br />
:ab 12.30 Uhr: Treffen im Congress Innsbruck, 3. Stock<br />
::13.00 Uhr: Begrüßung DDr. Petra Stöckl (Programm Managerin Life Sciences Tirol)<br />
::13.05 Uhr: Die neue Clusterstrategie. Vorstellung der Eckdaten<br />
::13.20 Uhr: Vorstellung der Clusterpartner und Diskussion der Clusterstrategie<br />
::* '''14.15 Uhr Vorstellung [[K-Regio MitoCom Tyrol|K-Regio MitoCom]]. Univ.-Prof. Dr. Erich Gnaiger (Oroboros)'''<br />
:15:00 m [http://www.standort-tirol.at/page.cfm?vpath=veranstaltungen/details&genericpageid=25359 Tiroler Innovationstag - Programm]<br />
<br />
<br />
=== 2011-11-19: An extension of MitoPedia ===<br />
<br />
* In addition to the [[List of respirometry terms]], the [[List of fluorometry terms]] and [[List of spectrophotometry terms]] were initiated by [[Harrison DK|David Harrison]].<br />
<br />
=== 2011-11-11: Bioblast alert ===<br />
<br />
* [[Bioblast alert]] was launched, for information purposes, not intended to be an advertisement.<br />
<br />
=== 2011-11-04: Product news ===<br />
<br />
* [[O2k-Fluorescence LED2-Module]]<br />
<br />
=== 2011-November: ''MitoCom Lectures'', Innsbruck ===<br />
<br />
* [[MitoCom lectures|Introduction to mitochondrial respiratory physiology]]<br />
<br />
=== 2011-10-20 to 21: Targeting Mitochondria, Berlin, DE ===<br />
* Invited lecture: Erich Gnaiger - [[Gnaiger 2011 Abstract-Berlin]]<br />
* Exhibition of OROBOROS INSTRUMENTS and ''MitoCom Tyrol'': Katrin Stecher, Lyda Staudacher<br />
<br />
=== 2011-09-24: High-output to replace wasteful high-throughput? ===<br />
<br />
* [[Talk:Rogers 2011 PlosOne|Discussion: OROBOROS O2k versus Seahorse XF]]<br />
<br />
=== 2011-09-05 to 04: MiP2011 Bordeaux, France ===<br />
<br />
* [http://www.mitophysiology.org 8th Conference on Mitochondrial Physiology] - MiP2011. Bordeaux, France.<br />
* Invited lecture: Erich Gnaiger - [[Sumbalova 2011 Abstract Bordeaux]]<br />
* Poster: Erich Gnaiger - [[Lemieux_2011_Abstract Bordeaux]]<br />
* Poster: Mario Fasching - [[Fasching_2011_Abstract Bordeaux]]<br />
* Exhibition of OROBOROS INSTRUMENTS and ''MitoCom Tyrol'': Katrin Stecher, Lyda Staudacher<br />
<br />
=== 2011-08-31 to 09-04: KAGOSHIMA JAPAN ===<br />
<br />
* 5th Biennial Meeting of Society for Free Radical Research (SFRR)-Asia, 8th Conference of Asian Society for Mitochondrial Research and Medicine (ASMRM), and [http://www.kagoshima2011.com 11th Conference of Japanese Society of Mitochondrial Research and Medicine (J-mit)]; Kagoshima Citizens´Culture Hall, Kagoshima, Japan. Chair: Prof. Hideyuki J. Majima; E-mail: mail@kagoshima2011.com<br />
* Invited lecture: Erich Gnaiger - [[Sumbalova 2011 Abstract Kagoshima]]<br />
* Exhibition of the OROBOROS Oxygraph-2k: Yoshiaki Nakayoshi, Sanyo Trading Co. Ltd., Tokyo, JP<br />
<br />
=== 2011-08-26 to 27: O2k-Workshop China ===<br />
* [http://www.oroboros.at/index.php?nextcourse#c3191 O2k-Workshop on High-Resolution Respirometry, Beijing and Tianjin, China]<br />
<br />
=== 2011-08-12: Circular on MitoScore ===<br />
<br />
'''[[MitoScore]]''' is an initiative of ''MitoCom'':<br />
* [[File:Network MitoScore.pdf]]<br />
<br />
=== 2011-08-09: Manuscript accepted for publication in IJBCB ===<br />
* "'''[[Lemieux 2011 IJBCB|Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart]]'''" (by Lemieux H, Semsroth S, Antretter H, Höfer D, Gnaiger E) was accepted for publication in ''Intl. J. Biochem. Cell Biol''. <br />
* From the anonymous referee's comments: <br />
** Reviewer #1: "''The manuscript by Lemieux and colleagues offers interesting '''insight into the bioenergetics of early phases of heart failure'''.''"<br />
** Reviewer #2: "'''''This is a beautifully written work, replete with well-designed experiments and, within the space limitations, a nice review of the pertinent literature.''' The demonstration of impaired mitochondrial fatty acid oxidation in early stages of human failure is very important, as is the demonstration of defects in the phosphorylation system and Complex I-linked respiration.''"<br />
** Reviewer #3: "'''''The manuscript belongs literally to the symphony of bioenergetics''' and have mastered on a maximum thorough and detailed level a priority study of human heart disease bioenergetics in an intermediate state of heart disease, which was done only scarcely in medical sciences and for this orthodox approach this work deserves a high attention. It elegantly describes detailed oxidative phosphorylation parameters in heart disease compared to the control human heart. Using high-resolution respirometry of permeabilized human heart muscle fibres authors progressively describe distinct aspect of measured data in logical steps to present important differences of diminished mitochondrial function during heart failure. Using elegant protocols developed for years, authors distinguish between phosphorylating (OXPHOS) and non-phosphorylating (ETS) conditions with both complex I and II input using physiological substrates of glucose and lipid oxidation. With presented protocols, authors are able to provide conclusions regarding, e.g., complex I impairment, phosphorylation system limitation (3.2) mitochondrial content (3.3), leak respiration involvement, etc., besides OXPHOS and ETS capacities. Authors use their own terminology describing respiratory states. It would be useful to explain briefly their terminology also in terms of the canonical state 2 - 4 terminology. In spite of this, the methodology approach and presented data are well explained.''"<br />
<br />
=== 2011-07-18: Informal ''MitoCom-MiPArt'' 18:18 ===<br />
* Monday, July 18 ● 18:18 h ● Schöpfstr. 18 – '''''we thank our guests for sharing a friendly 18:18 evening with us'''''.<br />
** Informal get-together at 'Oroboros' - science and art - celebrate a "''beautifully written work''" - a "''symphony of bioenergetics''" (see 2011-08-09).<br />
** Celebrate the official acceptance of [[MitoCom_K-Regio|K-Regio ''MitoCom Tyrol'']].<br />
** Explore future perspectives.<br />
<br />
=== 2011-07-12: The K-Regio MitoCom project - accepted ===<br />
*The project application [[MitoCom_K-Regio|''MitoCom Tyrol'']] was officially accepted.<br />
<br />
=== 2011-07-12: Manuscript accepted in AJP ===<br />
* "[[Pesta_2011_AJP|Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans" by Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E]], was accepted for publication in ''Am. J. Physiol. Regul. Integr. Comp. Physiol.'' (http://www.ncbi.nlm.nih.gov/pubmed/21775647). <br />
* From the anonymous referee's comments: ''This is really a superb revision; the MS has been improved substantially, particularly with reference to the theory and methods re: the HRR.''<br />
<br />
=== 2011-07-11 to 13: Introductory O2k-Workshop (IOC62) - MitoCom Tyrol ===<br />
* Location: OROBOROS INSTRUMENTS, Schöpfstr. 18, Innsbruck, Austria.<br />
* More informatio: [[IOC62]]<br />
<br />
=== 2011-06-15 to 18: UMDF Mitochondrial Medicine 2011 - Chicago, US ===<br />
* Poster: Erich Gnaiger - [[Burtscher 2011 AbstractUMDFChicago]]<br />
* Poster: Dominik Pesta - [[Pesta_2011_AbstractUMDFChicago-Training]]<br />
* Poster: Dominik Pesta - [[Pesta_2011_AbstractUMDFChicago-Diagnosis]]<br />
* Exhibition of OROBOROS INSTRUMENTS and ''MitoCom Tyrol'': Katrin Stecher, Dominik Pesta, Erich Gnaiger<br />
<br />
=== 2011-05-18: ''MiPArt'' - Mitochondria - the Aliens in our Cells === <br />
[http://www.mipart.at ''MiPArt'' - Mitochondria - the Aliens in our Cells] <br />
*Exhibition (Odra Noel, Richard Altmann and others) <br />
*Presentation of ''MitoCom Tyrol''<br />
<br />
=== 2011-05-01: Start K-Regio Project ''MitoCom Tyrol'' ===</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MitoCom_Events&diff=58239MitoCom Events2014-04-01T08:27:11Z<p>Bauer Christiane: </p>
<hr />
<div>{{MitoCom}}<br />
<br />
<br />
== ''MitoCom'' News ==<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
__TOC__<br />
<br />
== ''MitoCom'' Track Record 2014 ==<br />
<br />
=== Iditarod Alaska 2014 === <br />
<br />
Dr. Erich Gnaiger (Medical University of Innsbruck and OROBOROS INSTRUMENTS) and his collaborator Verena Laner (OROBOROS INSTRUMENTS) joined the project of [[Davis MS|Michael Davis]] (Oklahoma State University) in Big Lake, Alaska. Cumulative training effects on the mitochondrial function of alaskan sled dogs were studied at Happy Trails Kennel in Big Lake, Alaska. Further participants were [[Boushel RC|Robert Boushel]] (Institute for Sport and Health Sciences, Stockholm, SE) as well as Benjamin F. Miller and Karyn Hamilton (Colorado State University, Fort Collins, US). [http://www.oroboros.at/index.php?id=racing-dogs_alaska More info]<br />
<br />
=== O2k-Workshop on HRR and O2k-Fluorometry during [http://www.ntnu.edu/isb/enjoy-the-cold 'Enjoy the Cold'] in Spitsbergen, NO, March 27===<br />
Dr. Erich Gnaiger gave a presentation entitled ,Mitochondrial respiratory function in the cold‘ on March 26th.<br />
<br />
<br />
== ''MitoCom'' Track Record 2013 ==<br />
<br />
=== O2k-Workshop, Mumbai, India, January 31 ===<br />
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=== O2k-Workshop, Mahalabaleshwar, India, January 27-29 ===<br />
Lectures on HRR and Fluorometry were given by Dr. Erich Gnaiger during the 38th Seminar in Mahalabaleshwar.<br />
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=== O2k-Workshop, Bangalore, India, Dec 18 prior SMRM2013 ===<br />
A one-day O2k-Workshop on HRR and Fluorometry was given by Dr. Erich Gnaiger.<br />
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=== O2k-Fluorometry Workshop, Trondheim, Norway, Dec 10-11 ===<br />
* [[MiPNet18.11_IOC82|IOC82]]<br />
Dr. Erich Gnaiger and Verena Laner gave an O2k-Workshop, focused on the Fluorescence LED-2 Module prior the seminar 'Exercise in Medicine', which was organized by [http://www.ntnu.no/cerg CERG] of the NTNU University.<br />
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=== O2k-Luncheon Seminar, Seoul, Korea, Nov 05 @ ASMRM2013 ===<br />
* IOC81<br />
Dr. Erich Gnaiger (Medical University of Innsbruck) followed the invitation of Prof. Hong Kyu Lee to give a talk at the ASMRM2013 meeting. Furthermore he took this opportunity to give an O2k-Luncheon Workshop on HRR and Fluorometry.<br />
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=== O2k-Fluorometry Workshop, Schroecken, Austria, Oct 09 - 14 ===<br />
* [[MiPNet18.09_IOC80|IOC80]]<br />
A new concept, integrating the Flurometry-Workshop into the Basic-O2k-Workshop, was celebrated at the 80th O2k-Workshop in Schroecken. Tutors: Dr. Erich Gnaiger, Mona Fontana-Ayoub, Verena Laner and Gerhard Krumschnabel.<br />
<br />
<br />
=== O2k-Fluorometry Workshop, Obergurgl, Austria, September 19-23 ===<br />
* [[MiPNet18.07_IOC79|IOC79]]<br />
Prior MiP2013, a O2k-Fluorometry Workshop was given in Obergurgl by Dr. Erich Gnaiger, Dr. Mario Fasching, Mona Fontana-Ayoub and Verena Laner. Hands-on sessions for ~30 participants on 6 O2ks.<br />
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=== O2k-Workshop @ MiPsummer, Copenhagen, DK, August 27-29 ===<br />
* IOC78 - Tutors: Dr. Erich Gnaiger and Verena Laner<br />
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=== O2k-Luncheon Seminar @ UMDF, Newport Beach, CA, US, June 13 ===<br />
* IOC77<br />
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=== Prof. Gottfried Schatz === <br />
<br />
[https://www.i-med.ac.at/mypoint/thema/675045.html Article of the Medical University of Innsbruck]<br />
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=== O2k-Workshop, Prague, Austria, May 28 - 29===<br />
* [[MiPNet18.04_IOC76|IOC76]]<br />
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=== O2k-Workshop, Schroecken, Austria, Apr 09 - 14 ===<br />
* [[MiPNet18.03_IOC75|IOC75]]<br />
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=== Xtreme Everst 2 - high-altitude research in Nepal===<br />
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[[File: Erich_EBC.jpg|right|200px|Erich Gnaiger with 3 Oxygraphs at the Everest Base Camp]] <br />
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[[File: KTM_Andrew_Verena.jpg|right|200px|Verena Laner assisted Andrew Murray in the Kathamandu Laboratory]] <br />
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Dr. Erich Gnaiger and Verena Laner participated at the Xtreme Everest 2 Expedition in Nepal.<br />
<br />
'''Erich Gnaiger''' (Innsbruck, AT) joined the Xtreme Everest 2 mitochondrial team at Everest Base Camp (5,300 m) where acclimation to high altitude was studied using three O2k (March to May 2013). In Kathmandu (Nepal) at low altitude (1,400 m), Andrew Murray (Cambridge, UK) operated high-resolution respirometry on human musle biopsies with assistance by '''Verena Laner''' (Innsbruck, AT).<br />
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More infomation: [http://www.oroboros.at/?Xtreme-Everest2 Xtreme Everest 2]<br />
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Some impressions of the Expedition:<br />
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* [http://www.oroboros.at/?Everest-Base-Camp Everest Base Camp]<br />
* [http://www.oroboros.at/?KTM Kathmandu Laboratory]<br />
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The participants from Innsbruck are supported by the K-Regio project ''MitoCom Tyrol'', D. Swarovski Research Laboratory (Medical University of Innsbruck), and OROBOROS INSTRUMENTS.<br />
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=== O2k-TPP-Workshop, Innsbruck, Austria, Jan 22 - 25===<br />
* [[MiPNet18.02_IOC74|IOC74]]<br />
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=== O2k-Workshop, Kolkata, India, January 15 - 16===<br />
*[[MiPNet18.01_IOC73|IOC73]]<br />
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== ''MitoCom'' Track Record 2012 ==<br />
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=== O2k-Workshop, Schroecken, Austria, Dec 5 - 10 ===<br />
* [[IOC72]]<br />
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=== Bioblast 2012 - Conference on Mitochondrial Competence===<br />
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The Bioblast Conference 2012 took place in Hotel Grauer Bär from 10<sup>th</sup> to 12<sup>th</sup> December 2012.<br />
With more than 70 participants from 19 nations the conference was a great success.<br />
<br />
For the article of the Medical University Innsbruck, see: [https://www.i-med.ac.at/mypoint/news/668966.html MUI website]<br />
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More information: [[Bioblast 2012]]<br />
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=== Padua Muscle Days, Nov 23 - 25===<br />
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After the satellite meeting in Vienna on October 17th, Prof. Dr. Gnaiger was invited by Prof. Dr. Ugo Carraro (University of Padua) to Padua for a talk on high-resolution respirometry in permeabilized fibers from small biopsies of human muscle.<br />
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=== MCI Forum Technologie & Life Sciences, Innsbruck, Nov 6===<br />
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In her talk, Mag. Verena Laner will outline the development of the company OROBOROS INSTRUMENTS and will walk you through the early company days up to the current K-Regio Project ''MitoCom Tyrol''. [http://www.mci.edu/en/home/dokumentenverwaltung/category/164-pressemitteilung-20121115?download=3866%3Aprogrammflyer-forum-biotechnologie Programme]<br />
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=== Satellite Meeting Vienna, Oct 17 ===<br />
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Dr. Erich Gnaiger participated at the Satellite Meeting in Vienna, which took place on 17th October.<br />
<br />
Attendees of this meeting were Delegates of the University in Padua (Univ.-Prof. Dr. Ugo Carraro and Dr. Sandra Zampieri), University in Chieti (Univ.-Prof. Dr. Protasi) and University in Rome (Univ.-Prof. Dr. Andrea Musaro).<br />
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The meeting was organized by Dr. Kern from the Ludwig Boltzmann Institute in Vienna.<br />
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=== Standort Lunch Vienna, 2012 Sep 27 ===<br />
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Dr. Erich Gnaiger presented the ''MitoCom'' project at the Standort Lunch in Vienna, organized by the Standortagentur Tirol. [http://www.standort-tirol.at/page.cfm?vpath=veranstaltungen/details&genericpageid=35834 More information]. <br />
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The article of the STANDORT Tirol you can find in [http://www.standort-tirol.at/data.cfm?vpath=der_standort/standort-tirol_04-2012_final issue no. 15, page 8]<br />
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=== Medizintechnikforum Hall, 2012 Sep 20 to 21 ===<br />
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OROBOROS INSTRUMENTS exhibited at the [http://www.jahrestagung-oegbmt-2012.at/uploads/Programm_OEGBMT_Final.pdf Medizintechnikforum Hall], showing latest developments in high-resolution respirometry in combination with the O2k-Fluorescence LED2-Module. ''MitoCom Tyrol'' was presented together with the Open Innovation project, both supported by the Government of Tyrol.<br />
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=== Tiroltag, Europäisches Forum Alpbach, 2012 Aug 19 ===<br />
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Verena Laner, the project manager of MitoCom Tyrol, took part at the Tirolday, which was the "Opening" of the European Congress in Alpbach 2012. The main topic was "The future of sports in the Alps", which is an integral part of the ''MitoCom'' project. Additionally to the presentations and the award presentation ceremony for young scientists, there was a podium discussion with well-known experts such as the famous alpinist Reinhard Messner.<br />
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===''MitoCom'' Cooperation AltitudeOmics 2012, 2012 July 20 to Aug 13 ===<br />
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Dr. Erich Gnaiger participated in the [http://www.oroboros.at/?Bolivia2012 AltitudeOmics2012] project in Bolivia and provided the scientists with two O2ks and his expertise. Aim of the study was to investigate the impacts of hypoxia/acclimatization onto molecular mechanisms of the human cells.<br />
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[[File:BabyJoseph Visit Innsbruck.JPG|right|350px|Prof. Dr. Fr. Baby Joseph und Prof. Dr. Erich Gnaiger]] <br />
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=== Visiting scientist: Prof. Dr. Fr. Baby Joseph, July 19 ===<br />
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On July 19th, Prof. Dr. Fr. Baby Joseph visited the OROBOROS office.<br />
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[[Research_link_to_Prof_Baby_Joseph_India|Time schedule of the research visit]]<br />
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=== Houska Award 2011 - First price for ''MitoCom Tyrol'', April 26 ===<br />
* First price - Medical University of Innsbruck: http://www.oroboros.at/?Houska-Award_2011<br />
* http://www.houskapreis.at/siegerprojekt-2011-licht-in-die-kraftwerke-der-zellen<br />
* http://www.houskapreis.at<br />
* http://www.tt.com/Nachrichten/4702341-6/houska-preise-an-universit%C3%A4ten-in-innsbruck-und-graz.csp<br />
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=== Mitolab O2k-Fluorometry Workshop Madrid, Spain, May 7 ===<br />
* [http://vmbacterio.cbm.uam.es/mitolab/Programa.aspx?Opcion=Mitolab Mitolab Workshop] by [[Gnaiger E|Erich Gnaiger]]<br />
* Contact: Eduardo Rial (Email: rial@cib.csic.es) and [[Cadenas S|Susana Cadenas]] (Email: susana.cadenas@salud.madrid.org)<br />
<br />
>> See also [[MitoCom_Tyrol - Report]] '''<br />
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=== O2k-Workshop Schröcken, Austria, April 11 to 16 ===<br />
* [[IOC68]]<br />
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=== O2k-Workshop Sydney, Australia, April 2 to 4 ===<br />
* [http://www.oroboros.at/?IO67 '''IOC67''' - an International O2k-Workshop of ''MitoCom Tyrol''].<br />
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=== First O2k-Fluorometry Workshop, Innsbruck, March 15 to 16 ===<br />
* [http://www.oroboros.at/?IOC66 IOC66 O2k-Fluorometry Workshop]. An International O2k-Workshop of ''MitoCom Tyrol''.<br />
<br />
=== Manuscript accepted for publication in PloS One, March 9 ===<br />
* "'''[[Votion 2012 PLoS One|Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle]]'''" (by Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D) was accepted for publication in ''PloS One''. <br />
* From the anonymous referee's comments: <br />
** Reviewer #1: "This is an original work performed with state of the art methodologies, as high-resolution respirometry. The results indicate that not only mitochondrial capacity is a limiting factor of horse performance but also the modalities of energy substrate utilization. Horse is an animal of choice for energetic studies since it shows a huge consumption of oxygen during muscle contraction, suggesting an positive adaptation of the mitochondrial system as compared to other animals. .. This article provides new findings on the link between mitochondrial capacity and whole animal body size and aerobic capacity. It also clarifies whether mitochondrial capacity is a controling factor for horse performance. An important finding is also that the control by the phosphorylation system is stable and does not vary with the performance of the horse. In contrast, the relative utilization of complex I versus complex II varies witht he performance, and this is a new finding which adds up to our knowledge of energy regulation. This work is of broad interest for sport physiology, metabolic researchers and aging research."<br />
** Reviewer #2: "The results could be described in a much easier way if utilizing classical definitions of mitochondrial respiratory parameters (from the late Britton Chance's milestones), such as state IV (or II), state III, uncoupled respiration, respiratory control ratio and so on." (''Comment:'' [[Glossary: Respiratory states]])<br />
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== ''MitoCom'' Track Record 2011 ==<br />
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* 2011-12-10 to 15: [http://www.oroboros.at/index.php?nextcourse#c3124 Internatl. O2k-Workshop on High-Resolution Respirometry, Schröcken, Austria]<br />
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* 2011-12-08: [http://www.oroboros.at/index.php?nextcourse#c3202 O2k-Workshop on High-Resolution Respirometry, Hyderabad, India]<br />
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* See also: [http://www.oroboros.at/index.php?calendar Science Calendar]<br />
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=== 2011-11-29: 9. Tiroler Innovationstag ===<br />
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* 13:00-14:30 [http://www.standort-tirol.at/page.cfm?vpath=veranstaltungen/details&genericpageid=29311 Cluster-Partnertreffen LIFE SCIENCES Tirol]<br />
<br />
:'''Programm'''<br />
<br />
:ab 12.30 Uhr: Treffen im Congress Innsbruck, 3. Stock<br />
::13.00 Uhr: Begrüßung DDr. Petra Stöckl (Programm Managerin Life Sciences Tirol)<br />
::13.05 Uhr: Die neue Clusterstrategie. Vorstellung der Eckdaten<br />
::13.20 Uhr: Vorstellung der Clusterpartner und Diskussion der Clusterstrategie<br />
::* '''14.15 Uhr Vorstellung [[K-Regio MitoCom Tyrol|K-Regio MitoCom]]. Univ.-Prof. Dr. Erich Gnaiger (Oroboros)'''<br />
:15:00 m [http://www.standort-tirol.at/page.cfm?vpath=veranstaltungen/details&genericpageid=25359 Tiroler Innovationstag - Programm]<br />
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=== 2011-11-19: An extension of MitoPedia ===<br />
<br />
* In addition to the [[List of respirometry terms]], the [[List of fluorometry terms]] and [[List of spectrophotometry terms]] were initiated by [[Harrison DK|David Harrison]].<br />
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=== 2011-11-11: Bioblast alert ===<br />
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* [[Bioblast alert]] was launched, for information purposes, not intended to be an advertisement.<br />
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=== 2011-11-04: Product news ===<br />
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* [[O2k-Fluorescence LED2-Module]]<br />
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=== 2011-November: ''MitoCom Lectures'', Innsbruck ===<br />
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* [[MitoCom lectures|Introduction to mitochondrial respiratory physiology]]<br />
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=== 2011-10-20 to 21: Targeting Mitochondria, Berlin, DE ===<br />
* Invited lecture: Erich Gnaiger - [[Gnaiger 2011 Abstract-Berlin]]<br />
* Exhibition of OROBOROS INSTRUMENTS and ''MitoCom Tyrol'': Katrin Stecher, Lyda Staudacher<br />
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=== 2011-09-24: High-output to replace wasteful high-throughput? ===<br />
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* [[Talk:Rogers 2011 PlosOne|Discussion: OROBOROS O2k versus Seahorse XF]]<br />
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=== 2011-09-05 to 04: MiP2011 Bordeaux, France ===<br />
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* [http://www.mitophysiology.org 8th Conference on Mitochondrial Physiology] - MiP2011. Bordeaux, France.<br />
* Invited lecture: Erich Gnaiger - [[Sumbalova 2011 Abstract Bordeaux]]<br />
* Poster: Erich Gnaiger - [[Lemieux_2011_Abstract Bordeaux]]<br />
* Poster: Mario Fasching - [[Fasching_2011_Abstract Bordeaux]]<br />
* Exhibition of OROBOROS INSTRUMENTS and ''MitoCom Tyrol'': Katrin Stecher, Lyda Staudacher<br />
<br />
=== 2011-08-31 to 09-04: KAGOSHIMA JAPAN ===<br />
<br />
* 5th Biennial Meeting of Society for Free Radical Research (SFRR)-Asia, 8th Conference of Asian Society for Mitochondrial Research and Medicine (ASMRM), and [http://www.kagoshima2011.com 11th Conference of Japanese Society of Mitochondrial Research and Medicine (J-mit)]; Kagoshima Citizens´Culture Hall, Kagoshima, Japan. Chair: Prof. Hideyuki J. Majima; E-mail: mail@kagoshima2011.com<br />
* Invited lecture: Erich Gnaiger - [[Sumbalova 2011 Abstract Kagoshima]]<br />
* Exhibition of the OROBOROS Oxygraph-2k: Yoshiaki Nakayoshi, Sanyo Trading Co. Ltd., Tokyo, JP<br />
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=== 2011-08-26 to 27: O2k-Workshop China ===<br />
* [http://www.oroboros.at/index.php?nextcourse#c3191 O2k-Workshop on High-Resolution Respirometry, Beijing and Tianjin, China]<br />
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=== 2011-08-12: Circular on MitoScore ===<br />
<br />
'''[[MitoScore]]''' is an initiative of ''MitoCom'':<br />
* [[File:Network MitoScore.pdf]]<br />
<br />
=== 2011-08-09: Manuscript accepted for publication in IJBCB ===<br />
* "'''[[Lemieux 2011 IJBCB|Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart]]'''" (by Lemieux H, Semsroth S, Antretter H, Höfer D, Gnaiger E) was accepted for publication in ''Intl. J. Biochem. Cell Biol''. <br />
* From the anonymous referee's comments: <br />
** Reviewer #1: "''The manuscript by Lemieux and colleagues offers interesting '''insight into the bioenergetics of early phases of heart failure'''.''"<br />
** Reviewer #2: "'''''This is a beautifully written work, replete with well-designed experiments and, within the space limitations, a nice review of the pertinent literature.''' The demonstration of impaired mitochondrial fatty acid oxidation in early stages of human failure is very important, as is the demonstration of defects in the phosphorylation system and Complex I-linked respiration.''"<br />
** Reviewer #3: "'''''The manuscript belongs literally to the symphony of bioenergetics''' and have mastered on a maximum thorough and detailed level a priority study of human heart disease bioenergetics in an intermediate state of heart disease, which was done only scarcely in medical sciences and for this orthodox approach this work deserves a high attention. It elegantly describes detailed oxidative phosphorylation parameters in heart disease compared to the control human heart. Using high-resolution respirometry of permeabilized human heart muscle fibres authors progressively describe distinct aspect of measured data in logical steps to present important differences of diminished mitochondrial function during heart failure. Using elegant protocols developed for years, authors distinguish between phosphorylating (OXPHOS) and non-phosphorylating (ETS) conditions with both complex I and II input using physiological substrates of glucose and lipid oxidation. With presented protocols, authors are able to provide conclusions regarding, e.g., complex I impairment, phosphorylation system limitation (3.2) mitochondrial content (3.3), leak respiration involvement, etc., besides OXPHOS and ETS capacities. Authors use their own terminology describing respiratory states. It would be useful to explain briefly their terminology also in terms of the canonical state 2 - 4 terminology. In spite of this, the methodology approach and presented data are well explained.''"<br />
<br />
=== 2011-07-18: Informal ''MitoCom-MiPArt'' 18:18 ===<br />
* Monday, July 18 ● 18:18 h ● Schöpfstr. 18 – '''''we thank our guests for sharing a friendly 18:18 evening with us'''''.<br />
** Informal get-together at 'Oroboros' - science and art - celebrate a "''beautifully written work''" - a "''symphony of bioenergetics''" (see 2011-08-09).<br />
** Celebrate the official acceptance of [[MitoCom_K-Regio|K-Regio ''MitoCom Tyrol'']].<br />
** Explore future perspectives.<br />
<br />
=== 2011-07-12: The K-Regio MitoCom project - accepted ===<br />
*The project application [[MitoCom_K-Regio|''MitoCom Tyrol'']] was officially accepted.<br />
<br />
=== 2011-07-12: Manuscript accepted in AJP ===<br />
* "[[Pesta_2011_AJP|Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans" by Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E]], was accepted for publication in ''Am. J. Physiol. Regul. Integr. Comp. Physiol.'' (http://www.ncbi.nlm.nih.gov/pubmed/21775647). <br />
* From the anonymous referee's comments: ''This is really a superb revision; the MS has been improved substantially, particularly with reference to the theory and methods re: the HRR.''<br />
<br />
=== 2011-07-11 to 13: Introductory O2k-Workshop (IOC62) - MitoCom Tyrol ===<br />
* Location: OROBOROS INSTRUMENTS, Schöpfstr. 18, Innsbruck, Austria.<br />
* More informatio: [[IOC62]]<br />
<br />
=== 2011-06-15 to 18: UMDF Mitochondrial Medicine 2011 - Chicago, US ===<br />
* Poster: Erich Gnaiger - [[Burtscher 2011 AbstractUMDFChicago]]<br />
* Poster: Dominik Pesta - [[Pesta_2011_AbstractUMDFChicago-Training]]<br />
* Poster: Dominik Pesta - [[Pesta_2011_AbstractUMDFChicago-Diagnosis]]<br />
* Exhibition of OROBOROS INSTRUMENTS and ''MitoCom Tyrol'': Katrin Stecher, Dominik Pesta, Erich Gnaiger<br />
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=== 2011-05-18: ''MiPArt'' - Mitochondria - the Aliens in our Cells === <br />
[http://www.mipart.at ''MiPArt'' - Mitochondria - the Aliens in our Cells] <br />
*Exhibition (Odra Noel, Richard Altmann and others) <br />
*Presentation of ''MitoCom Tyrol''<br />
<br />
=== 2011-05-01: Start K-Regio Project ''MitoCom Tyrol'' ===</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MitoCom_Events&diff=58238MitoCom Events2014-04-01T08:26:39Z<p>Bauer Christiane: </p>
<hr />
<div>{{MitoCom}}<br />
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== ''MitoCom'' News ==<br />
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__TOC__<br />
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== ''MitoCom'' Track Record 2014 ==<br />
<br />
=== Iditarod Alaska 2014 === <br />
<br />
Dr. Erich Gnaiger (Medical University of Innsbruck and OROBOROS INSTRUMENTS) and his collaborator Verena Laner (OROBOROS INSTRUMENTS) joined the project of [[Davis MS|Michael Davis]] (Oklahoma State University) in Big Lake, Alaska. Cumulative training effects on the mitochondrial function of alaskan sled dogs were studied at Happy Trails Kennel in Big Lake, Alaska. Further participants were [[Boushel RC|Robert Boushel]] (Institute for Sport and Health Sciences, Stockholm, SE) as well as Benjamin F. Miller and Karyn Hamilton (Colorado State University, Fort Collins, US). [http://www.oroboros.at/index.php?id=racing-dogs_alaska More info]<br />
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=== O2k-Workshop on HRR and O2k-Fluorometry during [http://www.ntnu.edu/isb/enjoy-the-cold |'Enjoy the Cold'] in Spitsbergen, NO, March 27===<br />
Dr. Erich Gnaiger gave a presentation entitled ,Mitochondrial respiratory function in the cold‘ on March 26th.<br />
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== ''MitoCom'' Track Record 2013 ==<br />
<br />
=== O2k-Workshop, Mumbai, India, January 31 ===<br />
<br />
<br />
<br />
=== O2k-Workshop, Mahalabaleshwar, India, January 27-29 ===<br />
Lectures on HRR and Fluorometry were given by Dr. Erich Gnaiger during the 38th Seminar in Mahalabaleshwar.<br />
<br />
<br />
=== O2k-Workshop, Bangalore, India, Dec 18 prior SMRM2013 ===<br />
A one-day O2k-Workshop on HRR and Fluorometry was given by Dr. Erich Gnaiger.<br />
<br />
<br />
=== O2k-Fluorometry Workshop, Trondheim, Norway, Dec 10-11 ===<br />
* [[MiPNet18.11_IOC82|IOC82]]<br />
Dr. Erich Gnaiger and Verena Laner gave an O2k-Workshop, focused on the Fluorescence LED-2 Module prior the seminar 'Exercise in Medicine', which was organized by [http://www.ntnu.no/cerg CERG] of the NTNU University.<br />
<br />
<br />
=== O2k-Luncheon Seminar, Seoul, Korea, Nov 05 @ ASMRM2013 ===<br />
* IOC81<br />
Dr. Erich Gnaiger (Medical University of Innsbruck) followed the invitation of Prof. Hong Kyu Lee to give a talk at the ASMRM2013 meeting. Furthermore he took this opportunity to give an O2k-Luncheon Workshop on HRR and Fluorometry.<br />
<br />
<br />
=== O2k-Fluorometry Workshop, Schroecken, Austria, Oct 09 - 14 ===<br />
* [[MiPNet18.09_IOC80|IOC80]]<br />
A new concept, integrating the Flurometry-Workshop into the Basic-O2k-Workshop, was celebrated at the 80th O2k-Workshop in Schroecken. Tutors: Dr. Erich Gnaiger, Mona Fontana-Ayoub, Verena Laner and Gerhard Krumschnabel.<br />
<br />
<br />
=== O2k-Fluorometry Workshop, Obergurgl, Austria, September 19-23 ===<br />
* [[MiPNet18.07_IOC79|IOC79]]<br />
Prior MiP2013, a O2k-Fluorometry Workshop was given in Obergurgl by Dr. Erich Gnaiger, Dr. Mario Fasching, Mona Fontana-Ayoub and Verena Laner. Hands-on sessions for ~30 participants on 6 O2ks.<br />
<br />
<br />
=== O2k-Workshop @ MiPsummer, Copenhagen, DK, August 27-29 ===<br />
* IOC78 - Tutors: Dr. Erich Gnaiger and Verena Laner<br />
<br />
=== O2k-Luncheon Seminar @ UMDF, Newport Beach, CA, US, June 13 ===<br />
* IOC77<br />
<br />
<br />
<br />
=== Prof. Gottfried Schatz === <br />
<br />
[https://www.i-med.ac.at/mypoint/thema/675045.html Article of the Medical University of Innsbruck]<br />
<br />
=== O2k-Workshop, Prague, Austria, May 28 - 29===<br />
* [[MiPNet18.04_IOC76|IOC76]]<br />
<br />
<br />
=== O2k-Workshop, Schroecken, Austria, Apr 09 - 14 ===<br />
* [[MiPNet18.03_IOC75|IOC75]]<br />
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<br />
=== Xtreme Everst 2 - high-altitude research in Nepal===<br />
<br />
<br />
<br />
[[File: Erich_EBC.jpg|right|200px|Erich Gnaiger with 3 Oxygraphs at the Everest Base Camp]] <br />
<br />
[[File: KTM_Andrew_Verena.jpg|right|200px|Verena Laner assisted Andrew Murray in the Kathamandu Laboratory]] <br />
<br />
Dr. Erich Gnaiger and Verena Laner participated at the Xtreme Everest 2 Expedition in Nepal.<br />
<br />
'''Erich Gnaiger''' (Innsbruck, AT) joined the Xtreme Everest 2 mitochondrial team at Everest Base Camp (5,300 m) where acclimation to high altitude was studied using three O2k (March to May 2013). In Kathmandu (Nepal) at low altitude (1,400 m), Andrew Murray (Cambridge, UK) operated high-resolution respirometry on human musle biopsies with assistance by '''Verena Laner''' (Innsbruck, AT).<br />
<br />
<br />
More infomation: [http://www.oroboros.at/?Xtreme-Everest2 Xtreme Everest 2]<br />
<br />
<br />
Some impressions of the Expedition:<br />
<br />
* [http://www.oroboros.at/?Everest-Base-Camp Everest Base Camp]<br />
* [http://www.oroboros.at/?KTM Kathmandu Laboratory]<br />
<br />
<br />
<br />
The participants from Innsbruck are supported by the K-Regio project ''MitoCom Tyrol'', D. Swarovski Research Laboratory (Medical University of Innsbruck), and OROBOROS INSTRUMENTS.<br />
<br />
<br />
<br />
=== O2k-TPP-Workshop, Innsbruck, Austria, Jan 22 - 25===<br />
* [[MiPNet18.02_IOC74|IOC74]]<br />
<br />
<br />
=== O2k-Workshop, Kolkata, India, January 15 - 16===<br />
*[[MiPNet18.01_IOC73|IOC73]]<br />
<br />
<br />
<br />
<br />
<br />
== ''MitoCom'' Track Record 2012 ==<br />
<br />
<br />
=== O2k-Workshop, Schroecken, Austria, Dec 5 - 10 ===<br />
* [[IOC72]]<br />
<br />
<br />
=== Bioblast 2012 - Conference on Mitochondrial Competence===<br />
<br />
The Bioblast Conference 2012 took place in Hotel Grauer Bär from 10<sup>th</sup> to 12<sup>th</sup> December 2012.<br />
With more than 70 participants from 19 nations the conference was a great success.<br />
<br />
For the article of the Medical University Innsbruck, see: [https://www.i-med.ac.at/mypoint/news/668966.html MUI website]<br />
<br />
More information: [[Bioblast 2012]]<br />
<br />
<br />
<br />
=== Padua Muscle Days, Nov 23 - 25===<br />
<br />
After the satellite meeting in Vienna on October 17th, Prof. Dr. Gnaiger was invited by Prof. Dr. Ugo Carraro (University of Padua) to Padua for a talk on high-resolution respirometry in permeabilized fibers from small biopsies of human muscle.<br />
<br />
<br />
=== MCI Forum Technologie & Life Sciences, Innsbruck, Nov 6===<br />
<br />
In her talk, Mag. Verena Laner will outline the development of the company OROBOROS INSTRUMENTS and will walk you through the early company days up to the current K-Regio Project ''MitoCom Tyrol''. [http://www.mci.edu/en/home/dokumentenverwaltung/category/164-pressemitteilung-20121115?download=3866%3Aprogrammflyer-forum-biotechnologie Programme]<br />
<br />
<br />
=== Satellite Meeting Vienna, Oct 17 ===<br />
<br />
Dr. Erich Gnaiger participated at the Satellite Meeting in Vienna, which took place on 17th October.<br />
<br />
Attendees of this meeting were Delegates of the University in Padua (Univ.-Prof. Dr. Ugo Carraro and Dr. Sandra Zampieri), University in Chieti (Univ.-Prof. Dr. Protasi) and University in Rome (Univ.-Prof. Dr. Andrea Musaro).<br />
<br />
The meeting was organized by Dr. Kern from the Ludwig Boltzmann Institute in Vienna.<br />
<br />
<br />
=== Standort Lunch Vienna, 2012 Sep 27 ===<br />
<br />
Dr. Erich Gnaiger presented the ''MitoCom'' project at the Standort Lunch in Vienna, organized by the Standortagentur Tirol. [http://www.standort-tirol.at/page.cfm?vpath=veranstaltungen/details&genericpageid=35834 More information]. <br />
<br />
The article of the STANDORT Tirol you can find in [http://www.standort-tirol.at/data.cfm?vpath=der_standort/standort-tirol_04-2012_final issue no. 15, page 8]<br />
<br />
<br />
=== Medizintechnikforum Hall, 2012 Sep 20 to 21 ===<br />
<br />
OROBOROS INSTRUMENTS exhibited at the [http://www.jahrestagung-oegbmt-2012.at/uploads/Programm_OEGBMT_Final.pdf Medizintechnikforum Hall], showing latest developments in high-resolution respirometry in combination with the O2k-Fluorescence LED2-Module. ''MitoCom Tyrol'' was presented together with the Open Innovation project, both supported by the Government of Tyrol.<br />
<br />
<br />
=== Tiroltag, Europäisches Forum Alpbach, 2012 Aug 19 ===<br />
<br />
Verena Laner, the project manager of MitoCom Tyrol, took part at the Tirolday, which was the "Opening" of the European Congress in Alpbach 2012. The main topic was "The future of sports in the Alps", which is an integral part of the ''MitoCom'' project. Additionally to the presentations and the award presentation ceremony for young scientists, there was a podium discussion with well-known experts such as the famous alpinist Reinhard Messner.<br />
<br />
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===''MitoCom'' Cooperation AltitudeOmics 2012, 2012 July 20 to Aug 13 ===<br />
<br />
Dr. Erich Gnaiger participated in the [http://www.oroboros.at/?Bolivia2012 AltitudeOmics2012] project in Bolivia and provided the scientists with two O2ks and his expertise. Aim of the study was to investigate the impacts of hypoxia/acclimatization onto molecular mechanisms of the human cells.<br />
<br />
<br />
[[File:BabyJoseph Visit Innsbruck.JPG|right|350px|Prof. Dr. Fr. Baby Joseph und Prof. Dr. Erich Gnaiger]] <br />
<br />
=== Visiting scientist: Prof. Dr. Fr. Baby Joseph, July 19 ===<br />
<br />
On July 19th, Prof. Dr. Fr. Baby Joseph visited the OROBOROS office.<br />
<br />
[[Research_link_to_Prof_Baby_Joseph_India|Time schedule of the research visit]]<br />
<br />
=== Houska Award 2011 - First price for ''MitoCom Tyrol'', April 26 ===<br />
* First price - Medical University of Innsbruck: http://www.oroboros.at/?Houska-Award_2011<br />
* http://www.houskapreis.at/siegerprojekt-2011-licht-in-die-kraftwerke-der-zellen<br />
* http://www.houskapreis.at<br />
* http://www.tt.com/Nachrichten/4702341-6/houska-preise-an-universit%C3%A4ten-in-innsbruck-und-graz.csp<br />
<br />
=== Mitolab O2k-Fluorometry Workshop Madrid, Spain, May 7 ===<br />
* [http://vmbacterio.cbm.uam.es/mitolab/Programa.aspx?Opcion=Mitolab Mitolab Workshop] by [[Gnaiger E|Erich Gnaiger]]<br />
* Contact: Eduardo Rial (Email: rial@cib.csic.es) and [[Cadenas S|Susana Cadenas]] (Email: susana.cadenas@salud.madrid.org)<br />
<br />
>> See also [[MitoCom_Tyrol - Report]] '''<br />
<br />
=== O2k-Workshop Schröcken, Austria, April 11 to 16 ===<br />
* [[IOC68]]<br />
<br />
=== O2k-Workshop Sydney, Australia, April 2 to 4 ===<br />
* [http://www.oroboros.at/?IO67 '''IOC67''' - an International O2k-Workshop of ''MitoCom Tyrol''].<br />
<br />
=== First O2k-Fluorometry Workshop, Innsbruck, March 15 to 16 ===<br />
* [http://www.oroboros.at/?IOC66 IOC66 O2k-Fluorometry Workshop]. An International O2k-Workshop of ''MitoCom Tyrol''.<br />
<br />
=== Manuscript accepted for publication in PloS One, March 9 ===<br />
* "'''[[Votion 2012 PLoS One|Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle]]'''" (by Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D) was accepted for publication in ''PloS One''. <br />
* From the anonymous referee's comments: <br />
** Reviewer #1: "This is an original work performed with state of the art methodologies, as high-resolution respirometry. The results indicate that not only mitochondrial capacity is a limiting factor of horse performance but also the modalities of energy substrate utilization. Horse is an animal of choice for energetic studies since it shows a huge consumption of oxygen during muscle contraction, suggesting an positive adaptation of the mitochondrial system as compared to other animals. .. This article provides new findings on the link between mitochondrial capacity and whole animal body size and aerobic capacity. It also clarifies whether mitochondrial capacity is a controling factor for horse performance. An important finding is also that the control by the phosphorylation system is stable and does not vary with the performance of the horse. In contrast, the relative utilization of complex I versus complex II varies witht he performance, and this is a new finding which adds up to our knowledge of energy regulation. This work is of broad interest for sport physiology, metabolic researchers and aging research."<br />
** Reviewer #2: "The results could be described in a much easier way if utilizing classical definitions of mitochondrial respiratory parameters (from the late Britton Chance's milestones), such as state IV (or II), state III, uncoupled respiration, respiratory control ratio and so on." (''Comment:'' [[Glossary: Respiratory states]])<br />
<br />
<br />
== ''MitoCom'' Track Record 2011 ==<br />
<br />
* 2011-12-10 to 15: [http://www.oroboros.at/index.php?nextcourse#c3124 Internatl. O2k-Workshop on High-Resolution Respirometry, Schröcken, Austria]<br />
<br />
* 2011-12-08: [http://www.oroboros.at/index.php?nextcourse#c3202 O2k-Workshop on High-Resolution Respirometry, Hyderabad, India]<br />
<br />
<br />
* See also: [http://www.oroboros.at/index.php?calendar Science Calendar]<br />
<br />
=== 2011-11-29: 9. Tiroler Innovationstag ===<br />
<br />
* 13:00-14:30 [http://www.standort-tirol.at/page.cfm?vpath=veranstaltungen/details&genericpageid=29311 Cluster-Partnertreffen LIFE SCIENCES Tirol]<br />
<br />
:'''Programm'''<br />
<br />
:ab 12.30 Uhr: Treffen im Congress Innsbruck, 3. Stock<br />
::13.00 Uhr: Begrüßung DDr. Petra Stöckl (Programm Managerin Life Sciences Tirol)<br />
::13.05 Uhr: Die neue Clusterstrategie. Vorstellung der Eckdaten<br />
::13.20 Uhr: Vorstellung der Clusterpartner und Diskussion der Clusterstrategie<br />
::* '''14.15 Uhr Vorstellung [[K-Regio MitoCom Tyrol|K-Regio MitoCom]]. Univ.-Prof. Dr. Erich Gnaiger (Oroboros)'''<br />
:15:00 m [http://www.standort-tirol.at/page.cfm?vpath=veranstaltungen/details&genericpageid=25359 Tiroler Innovationstag - Programm]<br />
<br />
<br />
=== 2011-11-19: An extension of MitoPedia ===<br />
<br />
* In addition to the [[List of respirometry terms]], the [[List of fluorometry terms]] and [[List of spectrophotometry terms]] were initiated by [[Harrison DK|David Harrison]].<br />
<br />
=== 2011-11-11: Bioblast alert ===<br />
<br />
* [[Bioblast alert]] was launched, for information purposes, not intended to be an advertisement.<br />
<br />
=== 2011-11-04: Product news ===<br />
<br />
* [[O2k-Fluorescence LED2-Module]]<br />
<br />
=== 2011-November: ''MitoCom Lectures'', Innsbruck ===<br />
<br />
* [[MitoCom lectures|Introduction to mitochondrial respiratory physiology]]<br />
<br />
=== 2011-10-20 to 21: Targeting Mitochondria, Berlin, DE ===<br />
* Invited lecture: Erich Gnaiger - [[Gnaiger 2011 Abstract-Berlin]]<br />
* Exhibition of OROBOROS INSTRUMENTS and ''MitoCom Tyrol'': Katrin Stecher, Lyda Staudacher<br />
<br />
=== 2011-09-24: High-output to replace wasteful high-throughput? ===<br />
<br />
* [[Talk:Rogers 2011 PlosOne|Discussion: OROBOROS O2k versus Seahorse XF]]<br />
<br />
=== 2011-09-05 to 04: MiP2011 Bordeaux, France ===<br />
<br />
* [http://www.mitophysiology.org 8th Conference on Mitochondrial Physiology] - MiP2011. Bordeaux, France.<br />
* Invited lecture: Erich Gnaiger - [[Sumbalova 2011 Abstract Bordeaux]]<br />
* Poster: Erich Gnaiger - [[Lemieux_2011_Abstract Bordeaux]]<br />
* Poster: Mario Fasching - [[Fasching_2011_Abstract Bordeaux]]<br />
* Exhibition of OROBOROS INSTRUMENTS and ''MitoCom Tyrol'': Katrin Stecher, Lyda Staudacher<br />
<br />
=== 2011-08-31 to 09-04: KAGOSHIMA JAPAN ===<br />
<br />
* 5th Biennial Meeting of Society for Free Radical Research (SFRR)-Asia, 8th Conference of Asian Society for Mitochondrial Research and Medicine (ASMRM), and [http://www.kagoshima2011.com 11th Conference of Japanese Society of Mitochondrial Research and Medicine (J-mit)]; Kagoshima Citizens´Culture Hall, Kagoshima, Japan. Chair: Prof. Hideyuki J. Majima; E-mail: mail@kagoshima2011.com<br />
* Invited lecture: Erich Gnaiger - [[Sumbalova 2011 Abstract Kagoshima]]<br />
* Exhibition of the OROBOROS Oxygraph-2k: Yoshiaki Nakayoshi, Sanyo Trading Co. Ltd., Tokyo, JP<br />
<br />
=== 2011-08-26 to 27: O2k-Workshop China ===<br />
* [http://www.oroboros.at/index.php?nextcourse#c3191 O2k-Workshop on High-Resolution Respirometry, Beijing and Tianjin, China]<br />
<br />
=== 2011-08-12: Circular on MitoScore ===<br />
<br />
'''[[MitoScore]]''' is an initiative of ''MitoCom'':<br />
* [[File:Network MitoScore.pdf]]<br />
<br />
=== 2011-08-09: Manuscript accepted for publication in IJBCB ===<br />
* "'''[[Lemieux 2011 IJBCB|Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart]]'''" (by Lemieux H, Semsroth S, Antretter H, Höfer D, Gnaiger E) was accepted for publication in ''Intl. J. Biochem. Cell Biol''. <br />
* From the anonymous referee's comments: <br />
** Reviewer #1: "''The manuscript by Lemieux and colleagues offers interesting '''insight into the bioenergetics of early phases of heart failure'''.''"<br />
** Reviewer #2: "'''''This is a beautifully written work, replete with well-designed experiments and, within the space limitations, a nice review of the pertinent literature.''' The demonstration of impaired mitochondrial fatty acid oxidation in early stages of human failure is very important, as is the demonstration of defects in the phosphorylation system and Complex I-linked respiration.''"<br />
** Reviewer #3: "'''''The manuscript belongs literally to the symphony of bioenergetics''' and have mastered on a maximum thorough and detailed level a priority study of human heart disease bioenergetics in an intermediate state of heart disease, which was done only scarcely in medical sciences and for this orthodox approach this work deserves a high attention. It elegantly describes detailed oxidative phosphorylation parameters in heart disease compared to the control human heart. Using high-resolution respirometry of permeabilized human heart muscle fibres authors progressively describe distinct aspect of measured data in logical steps to present important differences of diminished mitochondrial function during heart failure. Using elegant protocols developed for years, authors distinguish between phosphorylating (OXPHOS) and non-phosphorylating (ETS) conditions with both complex I and II input using physiological substrates of glucose and lipid oxidation. With presented protocols, authors are able to provide conclusions regarding, e.g., complex I impairment, phosphorylation system limitation (3.2) mitochondrial content (3.3), leak respiration involvement, etc., besides OXPHOS and ETS capacities. Authors use their own terminology describing respiratory states. It would be useful to explain briefly their terminology also in terms of the canonical state 2 - 4 terminology. In spite of this, the methodology approach and presented data are well explained.''"<br />
<br />
=== 2011-07-18: Informal ''MitoCom-MiPArt'' 18:18 ===<br />
* Monday, July 18 ● 18:18 h ● Schöpfstr. 18 – '''''we thank our guests for sharing a friendly 18:18 evening with us'''''.<br />
** Informal get-together at 'Oroboros' - science and art - celebrate a "''beautifully written work''" - a "''symphony of bioenergetics''" (see 2011-08-09).<br />
** Celebrate the official acceptance of [[MitoCom_K-Regio|K-Regio ''MitoCom Tyrol'']].<br />
** Explore future perspectives.<br />
<br />
=== 2011-07-12: The K-Regio MitoCom project - accepted ===<br />
*The project application [[MitoCom_K-Regio|''MitoCom Tyrol'']] was officially accepted.<br />
<br />
=== 2011-07-12: Manuscript accepted in AJP ===<br />
* "[[Pesta_2011_AJP|Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans" by Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E]], was accepted for publication in ''Am. J. Physiol. Regul. Integr. Comp. Physiol.'' (http://www.ncbi.nlm.nih.gov/pubmed/21775647). <br />
* From the anonymous referee's comments: ''This is really a superb revision; the MS has been improved substantially, particularly with reference to the theory and methods re: the HRR.''<br />
<br />
=== 2011-07-11 to 13: Introductory O2k-Workshop (IOC62) - MitoCom Tyrol ===<br />
* Location: OROBOROS INSTRUMENTS, Schöpfstr. 18, Innsbruck, Austria.<br />
* More informatio: [[IOC62]]<br />
<br />
=== 2011-06-15 to 18: UMDF Mitochondrial Medicine 2011 - Chicago, US ===<br />
* Poster: Erich Gnaiger - [[Burtscher 2011 AbstractUMDFChicago]]<br />
* Poster: Dominik Pesta - [[Pesta_2011_AbstractUMDFChicago-Training]]<br />
* Poster: Dominik Pesta - [[Pesta_2011_AbstractUMDFChicago-Diagnosis]]<br />
* Exhibition of OROBOROS INSTRUMENTS and ''MitoCom Tyrol'': Katrin Stecher, Dominik Pesta, Erich Gnaiger<br />
<br />
=== 2011-05-18: ''MiPArt'' - Mitochondria - the Aliens in our Cells === <br />
[http://www.mipart.at ''MiPArt'' - Mitochondria - the Aliens in our Cells] <br />
*Exhibition (Odra Noel, Richard Altmann and others) <br />
*Presentation of ''MitoCom Tyrol''<br />
<br />
=== 2011-05-01: Start K-Regio Project ''MitoCom Tyrol'' ===</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MitoCom_Events&diff=58234MitoCom Events2014-03-31T14:47:02Z<p>Bauer Christiane: </p>
<hr />
<div>{{MitoCom}}<br />
<br />
<br />
== ''MitoCom'' News ==<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
__TOC__<br />
<br />
== ''MitoCom'' Track Record 2014 ==<br />
<br />
=== Iditarod Alaska 2014 === <br />
<br />
Dr. Erich Gnaiger (Medical University of Innsbruck and OROBOROS INSTRUMENTS) and his collaborator Verena Laner (OROBOROS INSTRUMENTS) joined the project of [[Davis MS|Michael Davis]] (Oklahoma State University) in Big Lake, Alaska. Cumulative training effects on the mitochondrial function of alaskan sled dogs were studied at Happy Trails Kennel in Big Lake, Alaska. Further participants were [[Boushel RC|Robert Boushel]] (Institute for Sport and Health Sciences, Stockholm, SE) as well as Benjamin F. Miller and Karyn Hamilton (Colorado State University, Fort Collins, US). [http://www.oroboros.at/index.php?id=racing-dogs_alaska More info]<br />
<br />
=== O2k-Workshop on HRR and O2k-Fluorometry during 'Enjoy the Cold' in Spitsbergen, NO, March 27===<br />
Dr. Erich Gnaiger gave a presentation entitled ,Mitochondrial respiratory function in the cold‘ on March 26th.<br />
<br />
<br />
<br />
== ''MitoCom'' Track Record 2013 ==<br />
<br />
=== O2k-Workshop, Mumbai, India, January 31 ===<br />
<br />
<br />
<br />
=== O2k-Workshop, Mahalabaleshwar, India, January 27-29 ===<br />
Lectures on HRR and Fluorometry were given by Dr. Erich Gnaiger during the 38th Seminar in Mahalabaleshwar.<br />
<br />
<br />
=== O2k-Workshop, Bangalore, India, Dec 18 prior SMRM2013 ===<br />
A one-day O2k-Workshop on HRR and Fluorometry was given by Dr. Erich Gnaiger.<br />
<br />
<br />
=== O2k-Fluorometry Workshop, Trondheim, Norway, Dec 10-11 ===<br />
* [[MiPNet18.11_IOC82|IOC82]]<br />
Dr. Erich Gnaiger and Verena Laner gave an O2k-Workshop, focused on the Fluorescence LED-2 Module prior the seminar 'Exercise in Medicine', which was organized by [http://www.ntnu.no/cerg CERG] of the NTNU University.<br />
<br />
<br />
=== O2k-Luncheon Seminar, Seoul, Korea, Nov 05 @ ASMRM2013 ===<br />
* IOC81<br />
Dr. Erich Gnaiger (Medical University of Innsbruck) followed the invitation of Prof. Hong Kyu Lee to give a talk at the ASMRM2013 meeting. Furthermore he took this opportunity to give an O2k-Luncheon Workshop on HRR and Fluorometry.<br />
<br />
<br />
=== O2k-Fluorometry Workshop, Schroecken, Austria, Oct 09 - 14 ===<br />
* [[MiPNet18.09_IOC80|IOC80]]<br />
A new concept, integrating the Flurometry-Workshop into the Basic-O2k-Workshop, was celebrated at the 80th O2k-Workshop in Schroecken. Tutors: Dr. Erich Gnaiger, Mona Fontana-Ayoub, Verena Laner and Gerhard Krumschnabel.<br />
<br />
<br />
=== O2k-Fluorometry Workshop, Obergurgl, Austria, September 19-23 ===<br />
* [[MiPNet18.07_IOC79|IOC79]]<br />
Prior MiP2013, a O2k-Fluorometry Workshop was given in Obergurgl by Dr. Erich Gnaiger, Dr. Mario Fasching, Mona Fontana-Ayoub and Verena Laner. Hands-on sessions for ~30 participants on 6 O2ks.<br />
<br />
<br />
=== O2k-Workshop @ MiPsummer, Copenhagen, DK, August 27-29 ===<br />
* IOC78 - Tutors: Dr. Erich Gnaiger and Verena Laner<br />
<br />
=== O2k-Luncheon Seminar @ UMDF, Newport Beach, CA, US, June 13 ===<br />
* IOC77<br />
<br />
<br />
<br />
=== Prof. Gottfried Schatz === <br />
<br />
[https://www.i-med.ac.at/mypoint/thema/675045.html Article of the Medical University of Innsbruck]<br />
<br />
=== O2k-Workshop, Prague, Austria, May 28 - 29===<br />
* [[MiPNet18.04_IOC76|IOC76]]<br />
<br />
<br />
=== O2k-Workshop, Schroecken, Austria, Apr 09 - 14 ===<br />
* [[MiPNet18.03_IOC75|IOC75]]<br />
<br />
<br />
=== Xtreme Everst 2 - high-altitude research in Nepal===<br />
<br />
<br />
<br />
[[File: Erich_EBC.jpg|right|200px|Erich Gnaiger with 3 Oxygraphs at the Everest Base Camp]] <br />
<br />
[[File: KTM_Andrew_Verena.jpg|right|200px|Verena Laner assisted Andrew Murray in the Kathamandu Laboratory]] <br />
<br />
Dr. Erich Gnaiger and Verena Laner participated at the Xtreme Everest 2 Expedition in Nepal.<br />
<br />
'''Erich Gnaiger''' (Innsbruck, AT) joined the Xtreme Everest 2 mitochondrial team at Everest Base Camp (5,300 m) where acclimation to high altitude was studied using three O2k (March to May 2013). In Kathmandu (Nepal) at low altitude (1,400 m), Andrew Murray (Cambridge, UK) operated high-resolution respirometry on human musle biopsies with assistance by '''Verena Laner''' (Innsbruck, AT).<br />
<br />
<br />
More infomation: [http://www.oroboros.at/?Xtreme-Everest2 Xtreme Everest 2]<br />
<br />
<br />
Some impressions of the Expedition:<br />
<br />
* [http://www.oroboros.at/?Everest-Base-Camp Everest Base Camp]<br />
* [http://www.oroboros.at/?KTM Kathmandu Laboratory]<br />
<br />
<br />
<br />
The participants from Innsbruck are supported by the K-Regio project ''MitoCom Tyrol'', D. Swarovski Research Laboratory (Medical University of Innsbruck), and OROBOROS INSTRUMENTS.<br />
<br />
<br />
<br />
=== O2k-TPP-Workshop, Innsbruck, Austria, Jan 22 - 25===<br />
* [[MiPNet18.02_IOC74|IOC74]]<br />
<br />
<br />
=== O2k-Workshop, Kolkata, India, January 15 - 16===<br />
*[[MiPNet18.01_IOC73|IOC73]]<br />
<br />
<br />
<br />
<br />
<br />
== ''MitoCom'' Track Record 2012 ==<br />
<br />
<br />
=== O2k-Workshop, Schroecken, Austria, Dec 5 - 10 ===<br />
* [[IOC72]]<br />
<br />
<br />
=== Bioblast 2012 - Conference on Mitochondrial Competence===<br />
<br />
The Bioblast Conference 2012 took place in Hotel Grauer Bär from 10<sup>th</sup> to 12<sup>th</sup> December 2012.<br />
With more than 70 participants from 19 nations the conference was a great success.<br />
<br />
For the article of the Medical University Innsbruck, see: [https://www.i-med.ac.at/mypoint/news/668966.html MUI website]<br />
<br />
More information: [[Bioblast 2012]]<br />
<br />
<br />
<br />
=== Padua Muscle Days, Nov 23 - 25===<br />
<br />
After the satellite meeting in Vienna on October 17th, Prof. Dr. Gnaiger was invited by Prof. Dr. Ugo Carraro (University of Padua) to Padua for a talk on high-resolution respirometry in permeabilized fibers from small biopsies of human muscle.<br />
<br />
<br />
=== MCI Forum Technologie & Life Sciences, Innsbruck, Nov 6===<br />
<br />
In her talk, Mag. Verena Laner will outline the development of the company OROBOROS INSTRUMENTS and will walk you through the early company days up to the current K-Regio Project ''MitoCom Tyrol''. [http://www.mci.edu/en/home/dokumentenverwaltung/category/164-pressemitteilung-20121115?download=3866%3Aprogrammflyer-forum-biotechnologie Programme]<br />
<br />
<br />
=== Satellite Meeting Vienna, Oct 17 ===<br />
<br />
Dr. Erich Gnaiger participated at the Satellite Meeting in Vienna, which took place on 17th October.<br />
<br />
Attendees of this meeting were Delegates of the University in Padua (Univ.-Prof. Dr. Ugo Carraro and Dr. Sandra Zampieri), University in Chieti (Univ.-Prof. Dr. Protasi) and University in Rome (Univ.-Prof. Dr. Andrea Musaro).<br />
<br />
The meeting was organized by Dr. Kern from the Ludwig Boltzmann Institute in Vienna.<br />
<br />
<br />
=== Standort Lunch Vienna, 2012 Sep 27 ===<br />
<br />
Dr. Erich Gnaiger presented the ''MitoCom'' project at the Standort Lunch in Vienna, organized by the Standortagentur Tirol. [http://www.standort-tirol.at/page.cfm?vpath=veranstaltungen/details&genericpageid=35834 More information]. <br />
<br />
The article of the STANDORT Tirol you can find in [http://www.standort-tirol.at/data.cfm?vpath=der_standort/standort-tirol_04-2012_final issue no. 15, page 8]<br />
<br />
<br />
=== Medizintechnikforum Hall, 2012 Sep 20 to 21 ===<br />
<br />
OROBOROS INSTRUMENTS exhibited at the [http://www.jahrestagung-oegbmt-2012.at/uploads/Programm_OEGBMT_Final.pdf Medizintechnikforum Hall], showing latest developments in high-resolution respirometry in combination with the O2k-Fluorescence LED2-Module. ''MitoCom Tyrol'' was presented together with the Open Innovation project, both supported by the Government of Tyrol.<br />
<br />
<br />
=== Tiroltag, Europäisches Forum Alpbach, 2012 Aug 19 ===<br />
<br />
Verena Laner, the project manager of MitoCom Tyrol, took part at the Tirolday, which was the "Opening" of the European Congress in Alpbach 2012. The main topic was "The future of sports in the Alps", which is an integral part of the ''MitoCom'' project. Additionally to the presentations and the award presentation ceremony for young scientists, there was a podium discussion with well-known experts such as the famous alpinist Reinhard Messner.<br />
<br />
<br />
===''MitoCom'' Cooperation AltitudeOmics 2012, 2012 July 20 to Aug 13 ===<br />
<br />
Dr. Erich Gnaiger participated in the [http://www.oroboros.at/?Bolivia2012 AltitudeOmics2012] project in Bolivia and provided the scientists with two O2ks and his expertise. Aim of the study was to investigate the impacts of hypoxia/acclimatization onto molecular mechanisms of the human cells.<br />
<br />
<br />
[[File:BabyJoseph Visit Innsbruck.JPG|right|350px|Prof. Dr. Fr. Baby Joseph und Prof. Dr. Erich Gnaiger]] <br />
<br />
=== Visiting scientist: Prof. Dr. Fr. Baby Joseph, July 19 ===<br />
<br />
On July 19th, Prof. Dr. Fr. Baby Joseph visited the OROBOROS office.<br />
<br />
[[Research_link_to_Prof_Baby_Joseph_India|Time schedule of the research visit]]<br />
<br />
=== Houska Award 2011 - First price for ''MitoCom Tyrol'', April 26 ===<br />
* First price - Medical University of Innsbruck: http://www.oroboros.at/?Houska-Award_2011<br />
* http://www.houskapreis.at/siegerprojekt-2011-licht-in-die-kraftwerke-der-zellen<br />
* http://www.houskapreis.at<br />
* http://www.tt.com/Nachrichten/4702341-6/houska-preise-an-universit%C3%A4ten-in-innsbruck-und-graz.csp<br />
<br />
=== Mitolab O2k-Fluorometry Workshop Madrid, Spain, May 7 ===<br />
* [http://vmbacterio.cbm.uam.es/mitolab/Programa.aspx?Opcion=Mitolab Mitolab Workshop] by [[Gnaiger E|Erich Gnaiger]]<br />
* Contact: Eduardo Rial (Email: rial@cib.csic.es) and [[Cadenas S|Susana Cadenas]] (Email: susana.cadenas@salud.madrid.org)<br />
<br />
>> See also [[MitoCom_Tyrol - Report]] '''<br />
<br />
=== O2k-Workshop Schröcken, Austria, April 11 to 16 ===<br />
* [[IOC68]]<br />
<br />
=== O2k-Workshop Sydney, Australia, April 2 to 4 ===<br />
* [http://www.oroboros.at/?IO67 '''IOC67''' - an International O2k-Workshop of ''MitoCom Tyrol''].<br />
<br />
=== First O2k-Fluorometry Workshop, Innsbruck, March 15 to 16 ===<br />
* [http://www.oroboros.at/?IOC66 IOC66 O2k-Fluorometry Workshop]. An International O2k-Workshop of ''MitoCom Tyrol''.<br />
<br />
=== Manuscript accepted for publication in PloS One, March 9 ===<br />
* "'''[[Votion 2012 PLoS One|Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle]]'''" (by Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D) was accepted for publication in ''PloS One''. <br />
* From the anonymous referee's comments: <br />
** Reviewer #1: "This is an original work performed with state of the art methodologies, as high-resolution respirometry. The results indicate that not only mitochondrial capacity is a limiting factor of horse performance but also the modalities of energy substrate utilization. Horse is an animal of choice for energetic studies since it shows a huge consumption of oxygen during muscle contraction, suggesting an positive adaptation of the mitochondrial system as compared to other animals. .. This article provides new findings on the link between mitochondrial capacity and whole animal body size and aerobic capacity. It also clarifies whether mitochondrial capacity is a controling factor for horse performance. An important finding is also that the control by the phosphorylation system is stable and does not vary with the performance of the horse. In contrast, the relative utilization of complex I versus complex II varies witht he performance, and this is a new finding which adds up to our knowledge of energy regulation. This work is of broad interest for sport physiology, metabolic researchers and aging research."<br />
** Reviewer #2: "The results could be described in a much easier way if utilizing classical definitions of mitochondrial respiratory parameters (from the late Britton Chance's milestones), such as state IV (or II), state III, uncoupled respiration, respiratory control ratio and so on." (''Comment:'' [[Glossary: Respiratory states]])<br />
<br />
<br />
== ''MitoCom'' Track Record 2011 ==<br />
<br />
* 2011-12-10 to 15: [http://www.oroboros.at/index.php?nextcourse#c3124 Internatl. O2k-Workshop on High-Resolution Respirometry, Schröcken, Austria]<br />
<br />
* 2011-12-08: [http://www.oroboros.at/index.php?nextcourse#c3202 O2k-Workshop on High-Resolution Respirometry, Hyderabad, India]<br />
<br />
<br />
* See also: [http://www.oroboros.at/index.php?calendar Science Calendar]<br />
<br />
=== 2011-11-29: 9. Tiroler Innovationstag ===<br />
<br />
* 13:00-14:30 [http://www.standort-tirol.at/page.cfm?vpath=veranstaltungen/details&genericpageid=29311 Cluster-Partnertreffen LIFE SCIENCES Tirol]<br />
<br />
:'''Programm'''<br />
<br />
:ab 12.30 Uhr: Treffen im Congress Innsbruck, 3. Stock<br />
::13.00 Uhr: Begrüßung DDr. Petra Stöckl (Programm Managerin Life Sciences Tirol)<br />
::13.05 Uhr: Die neue Clusterstrategie. Vorstellung der Eckdaten<br />
::13.20 Uhr: Vorstellung der Clusterpartner und Diskussion der Clusterstrategie<br />
::* '''14.15 Uhr Vorstellung [[K-Regio MitoCom Tyrol|K-Regio MitoCom]]. Univ.-Prof. Dr. Erich Gnaiger (Oroboros)'''<br />
:15:00 m [http://www.standort-tirol.at/page.cfm?vpath=veranstaltungen/details&genericpageid=25359 Tiroler Innovationstag - Programm]<br />
<br />
<br />
=== 2011-11-19: An extension of MitoPedia ===<br />
<br />
* In addition to the [[List of respirometry terms]], the [[List of fluorometry terms]] and [[List of spectrophotometry terms]] were initiated by [[Harrison DK|David Harrison]].<br />
<br />
=== 2011-11-11: Bioblast alert ===<br />
<br />
* [[Bioblast alert]] was launched, for information purposes, not intended to be an advertisement.<br />
<br />
=== 2011-11-04: Product news ===<br />
<br />
* [[O2k-Fluorescence LED2-Module]]<br />
<br />
=== 2011-November: ''MitoCom Lectures'', Innsbruck ===<br />
<br />
* [[MitoCom lectures|Introduction to mitochondrial respiratory physiology]]<br />
<br />
=== 2011-10-20 to 21: Targeting Mitochondria, Berlin, DE ===<br />
* Invited lecture: Erich Gnaiger - [[Gnaiger 2011 Abstract-Berlin]]<br />
* Exhibition of OROBOROS INSTRUMENTS and ''MitoCom Tyrol'': Katrin Stecher, Lyda Staudacher<br />
<br />
=== 2011-09-24: High-output to replace wasteful high-throughput? ===<br />
<br />
* [[Talk:Rogers 2011 PlosOne|Discussion: OROBOROS O2k versus Seahorse XF]]<br />
<br />
=== 2011-09-05 to 04: MiP2011 Bordeaux, France ===<br />
<br />
* [http://www.mitophysiology.org 8th Conference on Mitochondrial Physiology] - MiP2011. Bordeaux, France.<br />
* Invited lecture: Erich Gnaiger - [[Sumbalova 2011 Abstract Bordeaux]]<br />
* Poster: Erich Gnaiger - [[Lemieux_2011_Abstract Bordeaux]]<br />
* Poster: Mario Fasching - [[Fasching_2011_Abstract Bordeaux]]<br />
* Exhibition of OROBOROS INSTRUMENTS and ''MitoCom Tyrol'': Katrin Stecher, Lyda Staudacher<br />
<br />
=== 2011-08-31 to 09-04: KAGOSHIMA JAPAN ===<br />
<br />
* 5th Biennial Meeting of Society for Free Radical Research (SFRR)-Asia, 8th Conference of Asian Society for Mitochondrial Research and Medicine (ASMRM), and [http://www.kagoshima2011.com 11th Conference of Japanese Society of Mitochondrial Research and Medicine (J-mit)]; Kagoshima Citizens´Culture Hall, Kagoshima, Japan. Chair: Prof. Hideyuki J. Majima; E-mail: mail@kagoshima2011.com<br />
* Invited lecture: Erich Gnaiger - [[Sumbalova 2011 Abstract Kagoshima]]<br />
* Exhibition of the OROBOROS Oxygraph-2k: Yoshiaki Nakayoshi, Sanyo Trading Co. Ltd., Tokyo, JP<br />
<br />
=== 2011-08-26 to 27: O2k-Workshop China ===<br />
* [http://www.oroboros.at/index.php?nextcourse#c3191 O2k-Workshop on High-Resolution Respirometry, Beijing and Tianjin, China]<br />
<br />
=== 2011-08-12: Circular on MitoScore ===<br />
<br />
'''[[MitoScore]]''' is an initiative of ''MitoCom'':<br />
* [[File:Network MitoScore.pdf]]<br />
<br />
=== 2011-08-09: Manuscript accepted for publication in IJBCB ===<br />
* "'''[[Lemieux 2011 IJBCB|Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart]]'''" (by Lemieux H, Semsroth S, Antretter H, Höfer D, Gnaiger E) was accepted for publication in ''Intl. J. Biochem. Cell Biol''. <br />
* From the anonymous referee's comments: <br />
** Reviewer #1: "''The manuscript by Lemieux and colleagues offers interesting '''insight into the bioenergetics of early phases of heart failure'''.''"<br />
** Reviewer #2: "'''''This is a beautifully written work, replete with well-designed experiments and, within the space limitations, a nice review of the pertinent literature.''' The demonstration of impaired mitochondrial fatty acid oxidation in early stages of human failure is very important, as is the demonstration of defects in the phosphorylation system and Complex I-linked respiration.''"<br />
** Reviewer #3: "'''''The manuscript belongs literally to the symphony of bioenergetics''' and have mastered on a maximum thorough and detailed level a priority study of human heart disease bioenergetics in an intermediate state of heart disease, which was done only scarcely in medical sciences and for this orthodox approach this work deserves a high attention. It elegantly describes detailed oxidative phosphorylation parameters in heart disease compared to the control human heart. Using high-resolution respirometry of permeabilized human heart muscle fibres authors progressively describe distinct aspect of measured data in logical steps to present important differences of diminished mitochondrial function during heart failure. Using elegant protocols developed for years, authors distinguish between phosphorylating (OXPHOS) and non-phosphorylating (ETS) conditions with both complex I and II input using physiological substrates of glucose and lipid oxidation. With presented protocols, authors are able to provide conclusions regarding, e.g., complex I impairment, phosphorylation system limitation (3.2) mitochondrial content (3.3), leak respiration involvement, etc., besides OXPHOS and ETS capacities. Authors use their own terminology describing respiratory states. It would be useful to explain briefly their terminology also in terms of the canonical state 2 - 4 terminology. In spite of this, the methodology approach and presented data are well explained.''"<br />
<br />
=== 2011-07-18: Informal ''MitoCom-MiPArt'' 18:18 ===<br />
* Monday, July 18 ● 18:18 h ● Schöpfstr. 18 – '''''we thank our guests for sharing a friendly 18:18 evening with us'''''.<br />
** Informal get-together at 'Oroboros' - science and art - celebrate a "''beautifully written work''" - a "''symphony of bioenergetics''" (see 2011-08-09).<br />
** Celebrate the official acceptance of [[MitoCom_K-Regio|K-Regio ''MitoCom Tyrol'']].<br />
** Explore future perspectives.<br />
<br />
=== 2011-07-12: The K-Regio MitoCom project - accepted ===<br />
*The project application [[MitoCom_K-Regio|''MitoCom Tyrol'']] was officially accepted.<br />
<br />
=== 2011-07-12: Manuscript accepted in AJP ===<br />
* "[[Pesta_2011_AJP|Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans" by Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E]], was accepted for publication in ''Am. J. Physiol. Regul. Integr. Comp. Physiol.'' (http://www.ncbi.nlm.nih.gov/pubmed/21775647). <br />
* From the anonymous referee's comments: ''This is really a superb revision; the MS has been improved substantially, particularly with reference to the theory and methods re: the HRR.''<br />
<br />
=== 2011-07-11 to 13: Introductory O2k-Workshop (IOC62) - MitoCom Tyrol ===<br />
* Location: OROBOROS INSTRUMENTS, Schöpfstr. 18, Innsbruck, Austria.<br />
* More informatio: [[IOC62]]<br />
<br />
=== 2011-06-15 to 18: UMDF Mitochondrial Medicine 2011 - Chicago, US ===<br />
* Poster: Erich Gnaiger - [[Burtscher 2011 AbstractUMDFChicago]]<br />
* Poster: Dominik Pesta - [[Pesta_2011_AbstractUMDFChicago-Training]]<br />
* Poster: Dominik Pesta - [[Pesta_2011_AbstractUMDFChicago-Diagnosis]]<br />
* Exhibition of OROBOROS INSTRUMENTS and ''MitoCom Tyrol'': Katrin Stecher, Dominik Pesta, Erich Gnaiger<br />
<br />
=== 2011-05-18: ''MiPArt'' - Mitochondria - the Aliens in our Cells === <br />
[http://www.mipart.at ''MiPArt'' - Mitochondria - the Aliens in our Cells] <br />
*Exhibition (Odra Noel, Richard Altmann and others) <br />
*Presentation of ''MitoCom Tyrol''<br />
<br />
=== 2011-05-01: Start K-Regio Project ''MitoCom Tyrol'' ===</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=Talk:Bioblast_editorial_team&diff=58232Talk:Bioblast editorial team2014-03-31T13:03:57Z<p>Bauer Christiane: </p>
<hr />
<div>[[Image:B-Bioblast.png|left|30px|link=http://www.bioblast.at/index.php/Bioblast:About|Bioblast wiki]]<br />
== Popular Bioblast page ==<br />
[[Bioblast editorial board]] has been accessed more than<br />
:* 20,000 times (2013-12-07)<br />
:* 15,000 times (2013-06-25)<br />
:* 10,000 times (2012-12-26)<br />
:* 5,000 times (2012-04-24).<br />
<br />
<br />
<br />
== Bioblast user rights ==<br />
<br />
{| class="wikitable"<br />
|-<br />
! Name !! Institution !! User rights <br />
|-<br />
| [[Bauer CM|Bauer Christiane Maria]]||OROBOROS INSTRUMENTS, Innsbruck, AT||bureaucrat, Check user, administrator<br />
|-<br />
| [[Fasching M|Fasching Mario]]||OROBOROS INSTRUMENTS, Innsbruck, AT||bureaucrat, Check user, confirmed, administrator<br />
|-<br />
| [[Fleischmann S|Fleischmann Sandra]]||OROBOROS INSTRUMENTS, Innsbruck, AT||bureaucrat, Check user, administrator<br />
|-<br />
| [[Fontana-Ayoub M|Fontana-Ayoub Mona]]||OROBOROS INSTRUMENTS, Innsbruck, AT||administrator<br />
|-<br />
| [[Fritz K|Fritz Karina]]||OROBOROS INSTRUMENTS, Innsbruck, AT||bureaucrat, Check user, administrator<br />
|-<br />
| [[Gnaiger E|Gnaiger Erich]]||OROBOROS INSTRUMENTS, Innsbruck, AT||bureaucrat, Check user, confirmed, administrator<br />
|-<br />
| [[Harrison DK|Harrison David K]]||OROBOROS INSTRUMENTS, Innsbruck, AT||administrator<br />
|-<br />
| [[Hoppel F|Hoppel Florian]]||OROBOROS INSTRUMENTS, Innsbruck, AT||bureaucrat, Check user, administrator<br />
|-<br />
| [[Krumschnabel G|Krumschnabel Gerhard]]||OROBOROS INSTRUMENTS, Innsbruck, AT||bureaucrat, Check user, administrator<br />
|-<br />
| [[Gruber V|Gruber Verena]]||OROBOROS INSTRUMENTS, Innsbruck, AT||bureaucrat, Check user, administrator<br />
|-<br />
| [[Marte V|Marte Verena]]||OROBOROS INSTRUMENTS, Innsbruck, AT||bureaucrat, Check user, administrator<br />
|-<br />
| [[Oroboros|Oroboros ]]||OROBOROS INSTRUMENTS, Innsbruck, AT||bureaucrat, Check user, confirmed, administrator<br />
|-<br />
|}<br />
<br />
<br />
<br />
== Bioblast editorial board: Past members ==<br />
<br />
* Administrator: [[Meissner B]] (2012-2013)<br />
<br />
* Administrator: [[Wiethuechter A]] (2010-2011)</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MiP2014&diff=58231MiP20142014-03-31T10:10:00Z<p>Bauer Christiane: </p>
<hr />
<div>[[Image:IUBMB-logo.gif|left|100px|link=http://www.iubmb.org|IUBMB - ''International Union of Biochemistry and Molecular Biology'']]<br />
[[Image:MiPsocietyLOGO.JPG|right|200px|link=http://www.mitophysiology.org|MiPsociety]]<br />
[http://www.mitophysiology.org/?MiP2014 '''<big>MiP2014 - 10<sup>th</sup> MiP''conference'': Joint IUBMB/MiP Symposium on Mitochondrial Physiology - a Point/Counterpoint Meeting</big>''']<br />
<br />
'''''International Union of Biochemistry and Molecular Biology''''' and '''Mitochondrial Physiology Society'''<br />
<br />
Obergurgl, Tyrol, Austria. 2014-Sep-08 to 12<br />
== MiP2014 - a point/counterpoint meeting ==<br />
<br />
Mitochondrial research has recently undergone a dramatic renaissance, due to numerous discoveries revealing its central role in cell death, disease pathology, aging, thermogenesis, oxidative stress, cell signalling, cellular regulation and cell bioenergetics. The aim of the small symposium is to bring together experts in mitochondrial research spanning from the biochemical and molecular level to the interactions within the cell. In addition to the latest scientific findings, new methodological developments in measuring mitochondrial function and dysfunction are presented. A point/counterpoint format is chosen to discuss selected hot topics and some fundamental methodological and conceptual issues.<br />
<br />
The expected attendance is max. 100 participants. ->> Compare [[MiP2013 Programme]]<br />
Abstracts and presentations: refer to [http://www.chem.qmul.ac.uk/iubmb/ IUBMB nomenclature]; >> [[MitoPedia Glossary: Respiratory states|MitoPedia Glossary: '''Respiratory states''']]<br />
<br />
== Programme structure ==<br />
<br />
In each of the 12 sessions a point/counterpoint introduction will be given by invited keynote speakers to provide a balanced overview and promote discussions. Short oral presentations are selected from abstracts, including student presentations. Rapid platform statements will introduce posters which contribute to a specific session. Posters are up throughout the meeting, and poster discussions are integrated into the platform sessions.<br />
<br />
=== A. Mitochondrial respiratory control === <br />
* A1. mtDNA, mt-phenotype and disease – is there biochemical and molecular-genetic evidence to explain observed associations? <br />
* A2. Fission and fusion, mitochondrial structure and function - is there a tight connection? <br />
* A3. Mitochondrial density and normalization of respiration – is there a general biochemical or molecular marker? <br />
* A4. Biochemical reserve capacity, respiratory control ratios and coupling efficiency – are conventional concepts inconsistent?<br />
<br />
=== B. Experimental protocols in mitochondrial biochemistry === <br />
* B1. Incubation media for mt-preparations and experimental temperature in functional studies of mammalian mitochondria – guided by concept or tradition? <br />
* B2. Intracellular oxygen pressure versus oxygen regimes in studies of ROS production and respiration - are mitochondria sources or sinks of ROS?<br />
* B3. Quantitative measurement of mt-membrane potential - potentiometric/fluorometric. <br />
* B4. Instrumental platforms in mitochondrial physiology – industrial presentations.<br />
<br />
=== C. Towards quality assurance === <br />
* C1. Do blood cells versus muscle biopsies provide valid models for diagnosis of mitochondrial pathologies? <br />
* C2. Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
* C3. From availability and quality control of chemicals to mitochondrial respiratory protocols – who controls? <br />
* C4. Standards for the diagnosis of mitochondrial pathologies – a challenge for collaboration with mitochondrial organizations?<br />
Particularly in view of Subtopic C, it may be attractive for mitochondrial organizations to send and support delegates. The conference will, therefore, provide a unique forum for international networking, and may inspire new national groups to be formed in contact with established mitochondrial organizations:<br />
>> '''[[MitoGlobal_Organizations]]'''<br />
<br />
== MiP2014 preliminary participants ==<br />
<br />
in progress<br />
<br />
{| class="wikitable"<br />
|-<br />
! Name !! Institution !! Preliminary title <br />
|-<br />
| [[Ali SS|Ali Sameh S]]||Zewail City for Science and Technology, ET||Oxygen-consuming and ROS-producing activities in synaptosomes.<br />
|-<br />
| [[Bauer CM|Bauer Christiane Maria]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Borutaite_V|Borutaite Vilmante]]||Lithuanian University of Health Sciences, Kaunas, LT||Controversies in testing integrity of mitochondrial membranes.<br />
|-<br />
| [[Brown D|Brown David]]||East Carolina University, NC, US||<br />
|-<br />
| [[Contreras-Ferrat A|Contreras-Ferrat Ariel]]||University of Chile, CL||<br />
|-<br />
| [[Coen P|Coen Paul]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Dohlmann T|Dohlmann Tine]]||University of Copenhagen, DK||<br />
|-<br />
| [[Elmer E|Elmer Eskil]]||Wallenberg Neuroscience Center, Lund, SE||Ex vivo respiratory capacity of human blood cells - a SUITable application of the O2k Oxygraph / A novel pharmacological approach to counteract metabolic crisis.<br />
|-<br />
| [[Felding-Habermann B|Felding-Habermann Brunhilde]]||The Scripps Research Institute, La Jolla, CA, US||Mitochondrial functions in breast cancer progression.<br />
|-<br />
| [[Goodpaster BH|Goodpaster Bret]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Gnaiger E|Gnaiger Erich]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Grefte S|Grefte Sander]]||Radboud University Nijmegen Medical Centre, Nijmegen, NL||Pathophysiology of mitochondrial (dys)function in skeletal muscle.<br />
|-<br />
| [[Hahn D|Hahn Dagmar]]||Inselspital Bern, CH||Validation of Oxygen Consumption measurements in fibroblasts from patients with mitochondrial diseases.<br />
|-<br />
| [[Hand SC|Hand Steven C]]||Louisiana State University, LA, US||Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
|-<br />
| [[Harrison DK|Harrison David K]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Hepple_RT| Hepple Russel T.]]||McGill University, Montreal, CA||Mitochondrial involvement in aging muscle: cause and effect(or).<br />
|-<br />
| [[Hey-Mogensen M|Hey-Mogensen Martin]]||Novo Nordisk A/S Diabetes NBEs & Obesity Biology, Måløv, DK||<br />
|-<br />
| [[Jana Prado F|Jana Prado Fabian]]||University of Chile, CL||<br />
|-<br />
| [[Kane DA| Kane Daniel A.]]||St. Francis Xavier University, Antigonish, CA||Mitochondrial shuttles in muscle.<br />
|-<br />
| [[Kraunsoe R|Kraunsoe Regitze]]||University of Copenhagen, DK||<br />
|-<br />
| [[Koopman WJ|Koopman Werner J]]||Radboud University Nijmegen Medical Centre, NL||Redox regulation of mitochondrial dynamics.<br />
|-<br />
| [[Kowaltowski AJ|Kowaltowski Alicia J.]]||University of Sao Paulo, BR||<br />
|-<br />
| [[Laner V|Laner Verena]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Larsen S|Larsen Steen]]||University of Copenhagen, DK||Normalization of respiration.<br />
|-<br />
| [[Larsson NG|Göran-Larsson Nils]]||Max Planck Institute for the Biology of Ageing, Cologne, DE||Mouse models with mtDNA mutations.<br />
|-<br />
| [[Ojuka E|Ojuka Edward]]||University of Cape Town, ZA||Fission and fusion, mitochondrial structure and function - is there a tight connection?<br />
|-<br />
| [[Perry CG|Perry Christopher G]]||York University, Ontario, CA||Re-visiting critical experimental parameters to control when measuring respiratory kinetics in permeabilized muscle fibres.<br />
|-<br />
| [[Pesta D|Pesta Dominik]]||Yale University School of Medicine, CT, US||<br />
|- <br />
| [[Picard_M|Picard Martin]]||University of Pennsylvania, PA, US||Quantitative regulation of nuclear gene expression by mitochondrial DNA<br />
heteroplasmy.<br />
|-<br />
| [[Pecina P|Pecina Petr]]||Academy of Sciences of the Czech Republic, Prague, CZ||The use of lymphocytes for diagnostics of mitochondrial disorders.<br />
|-<br />
| [[Purhonen J|Purhonen Janne]]||University Helsinki, FI||<br />
|-<br />
| [[Ristow M|Ristow Michael]]||ETH Zurich, CH||Promoting health and lifespan by increasing mitochondrial ROS formation.<br />
|-<br />
| [[Shirihai O|Shirihai Orian]]||The Shirihai Lab at Boston University, MA, US||<br />
|-<br />
| [[Skulachev VP|Skulachev Vladimir]]||Lomonosov Moscow State University, Moscow, RU||Mitochondria are sources of ROS rather than sinks: Physiological effects of mitochondria-targeted antioxidants.<br />
|-<br />
| [[Starkov AA|Starkov Anatoly]]||Weill Medical College Cornell University, NY, US||<br />
|-<br />
| [[Stiban J|Stiban Johnny]]||Birzeit University, Ramallah, PS||Very long chain ceramides interfere with C16-ceramide-induced channel formation: A plausible mechanism for regulating the initiation of intrinsic apoptosis.<br />
|-<br />
| [[Zorzano A|Zorzano Antonio]]||Universitat de Barcelona, ES||Metabolic implications of mitofusin 2 dysfunction.<br />
|-<br />
| [[Sumbalova Z|Sumbalova Zuzana]]||Comenius University, Bratislava, SK||<br />
|}<br />
<br />
== Venue ==<br />
<br />
The symposium will be hosted in the '''University Center in Obergurgl''' 100 km from Innsbruck. The closest airport is in Innsbruck. Participants arriving at Munich airport will take the train or an airport shuttle to Innsbruck. We provide support in arranging the transfer between Munich and Innsbruck. A bus transfer is organized between Innsbruck and Obergurgl.<br />
<br />
The Obergurgl University Center is located at an altitude of 1940 meters above sea-level. The historical buildings, carefully refurbished and equipped with the latest technical systems, can accommodate around 70 persons in 43 bedrooms and includes conference rooms, the Alpine Research Center, laboratory, sports medicine, lounges and the restaurant. All rooms are equipped with an Internet connection. Additional participants are accommodated in smaller guest houses in 5 minutes walking distance. Meals are served for all participants in the University Center. Two conference rooms for a total of 200 persons and three small rooms for round table discussions are available. There is enough space for poster presentations and instrument exhibitions. The cosy bar and lounge area with an open fire place adds to the familiar atmosphere and supports the interactive spirit of the symposium.<br />
<br />
The small village of Obergurgl is situated in an impressive landscape at about 1900 m above sea level in the Ötz valley. It is surrounded by mountains, offering an exceptional view to the Ötz valley glaciers. During summer time the area is famous for hiking and for skiing in winter time. On the doorstep: one of Europe´s finest Biosphere Parks. The unique surroundings offer the possibility of combining scientific discussions while exploring the alpine environment during the famous “walks and talks”. The informal atmosphere of the workshop encourages also students and young post-docs to participate actively in scientific discussions and to develop future collaborations.<br />
<br />
University Centre Obergurgl, Gaisbergweg 5, 6456 Obergurgl, Tyrol, Austria.<br />
Tel.: +43 (0) 512 507 37201 | Fax: +43 (0) 512 507 37400 | Email: obergurgl@uibk.ac.at <br />
<br />
== International programme committee ==<br />
The international programme committee will provide scientific input by active participation, nominate further invited speakers to ensure balanced point/counterpoint introductions, and select the abstracts as a basis for participation (restricted number of participants), for oral 10+5 min presentations, and for flash presentations.<br />
<br />
Chair:<br />
* [[Gnaiger E|Gnaiger Erich]], Medical University Innsbruck, AT. - erich.gnaiger@mitophysiology.org<br />
Members:<br />
* [[Ali SS|Ali Sameh S]], Helmy Institute of Medical Sciences, Giza, Egypt<br />
* [[Borutaite V|Borutaite Vilma]], Lithuanian University of Health Sciences Kaunas, LT<br />
* [[Neurocon|Chakrabarti Sasanka]], Institute of Post-graduate Medical Education & Research, Kolkatta, India<br />
* [[Chinese Society of Mitochondrial Research and Medicine|Chen Quan]], Chinese Academy of Sciences, Nankai University, Beijing <br />
* [[Dela F|Dela Flemming]], University of Copenhagen, DK <br />
* [[Duchen MR|Duchen Michael R]], University College London, UK<br />
* [[Elmer E|Elmér Eskil]], Lund University, SE <br />
* [[Hand SC|Hand Steven]], Louisiana State University, LA, US<br />
* [[Hickey AJ|Hickey Anthony J]], University of Auckland, NZ<br />
* [[Koopman WJ|Koopman Werner J]], Radboud Univ Nijmegen Medical Centre, NL<br />
* [[Tanaka M|Tanaka Masashi]], Toyko Metropolitan Institute of Gerontology, JP<br />
* [[Neufer PD|Neufer P Darrell]], East Carolina University, Greenville, NC, US<br />
* [[Ojuka E|Ojuka Edward]], University of Cape Town, South Africa<br />
* [[Pecina P|Pecina Petr]], Academy of Sciences of the Czech Republic, CZ<br />
* [[Perry CG|Perry Christopher G]], York University, Toronto, Canada<br />
* [[Rossignol R|Rossignol Rodrigue]], Université Victor Segalen-Bordeaux, FR<br />
* [[Vercesi AE|Vercesi Anibal E]], State University of Campinas, Sao Paulo, Brazil<br />
* [[Taiwan Society for Mitochondrial Research and Medicine|Wei Yau-Huei]], Mackay Medical College, New Taipei City, Taiwan<br />
* [[Zorzano A|Zorzano Antonio]], Universitat de Barcelona, Spain<br />
<br />
== Information for invited speakers==<br />
<br />
MiP2014 will be held at the University Centre Obergurgl, with maximum capacity of 124 persons and a price per person per night of € 65.- including a shared double room, breakfast, and dinner. MiP''conferences'' are organized as small meetings facilitating communication between senior and junior participants. In the tradition of MiP''conferences'', we are asking invited speakers to cooperate and try to receive local support for joining us at the prestigious Mitochondrial Physiology Conference (limited to 124 participants). Support will be provided to students and young investigators.<br />
<br />
== Post-conference O2k-Workshop ==<br />
<br />
A [http://www.oroboros.at/?IOC_2014-09_obergurgl post-conference workshop] is organized on the same venue, for hands-on training in mitochondrial respiratory physiology, introducing the combined measurement of high-resolution respirometry and fluorometry (OROBOROS INSTRUMENTS). Since participants come from all-over-the-world, including many students, the combination of the symposium with the workshop makes travelling more economical for the maximum of 24 workshop-participants. The post-conference workshop is financially completely separate from the symposium.<br />
<br />
[[File:MitoGlobal.jpg|left|40px|MitoGlobal]]<br />
== MiP''events'' ==<br />
are listed as '''[[MitoGlobal_Events]]'''.<br />
<br />
<br />
<br />
<br />
<br />
[[Category:MitoPedia]]<br />
[[Category:All]]</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=Talk:Bioblast&diff=58209Talk:Bioblast2014-03-28T14:36:23Z<p>Bauer Christiane: </p>
<hr />
<div>== Bioblast log in or create your user account ==<br />
::[[Create Account|'''Create your user account''']] / [[Special:UserLogin|Log in]]<br />
<br />
<br />
<br />
[[Image:B-Bioblast.png|left|30px|link=http://www.bioblast.at/index.php/Bioblast:About|Bioblast wiki]]<br />
== Popular Bioblast page ==<br />
<br />
The [[Bioblast|Bioblast wiki startpage]] has been accessed more than<br />
* 240,000 times (2014-03-03)<br />
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* 20,000 times (2011-10-05)</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=Bioblast_editorial_team&diff=58208Bioblast editorial team2014-03-28T14:25:17Z<p>Bauer Christiane: /* Bioblast advisory board */</p>
<hr />
<div>[[Image:B-Bioblast.png|left|70px|link=http://www.bioblast.at/index.php/Bioblast:About|Bioblast wiki]]<br />
= Bioblast editors =<br />
<br />
== Bioblast advisory board ==<br />
<br />
* Editor-in-chief: [[Gnaiger E]], OROBOROS INSTRUMENTS Corp, Innsbruck, Austria, and Medical University of Innsbruck, Innsbruck, Austria. Email: erich.gnaiger@oroboros.at (2010-)<br />
* Co-Editors: <br />
: [[Fleischmann S]], [mailto:sandra.fleischmann@oroboros.at sandra.fleischmann@oroboros.at] (2014-)<br />
: [[Laner V]], [mailto:verena.laner@oroboros.at verena.laner@oroboros.at] (2013-)<br />
: [[Bauer CM]], [mailto:christiane.bauer@oroboros.at christiane.bauer@oroboros.at] (2013-)<br />
: [[Krumschnabel G]], [mailto:gerhard.krumschnabel@oroboros.at gerhard.krumschnabel@oroboros.at] (2013-)<br />
* Administrators<br />
: [[Marte V]], [mailto:verena.marte@oroboros.at verena.marte@oroboros.at] (2013-)<br />
: [[Fasching M]], [mailto:mario.fasching@oroboros.at mario.fasching@oroboros.at] (2010-)<br />
<br />
== Bioblast editorial board ==<br />
<br />
=== Bioblast section editors ===<br />
<br />
* [[O2k-Publications]]: [[Krumschnabel G]] with [[Hoppel F]], [[Heidler J]] - [[Editors O2k-Publications]]<br />
* [[List of terms and abbreviations]]: [[Gnaiger E]] with [[Krumschnabel G]], [[Harrison DK]], [[Heidler J]], [[Laner V]] - [[MitoPedia_Glossaries]]<br />
* [[Library of protocols]]: - ''in preparation''<br />
* [[Gentle Science]]: [[Gnaiger E]]<br />
<br />
<br />
* [[MiPNet_Laboratories|OROBOROS MiPNet_Laboratories]]: [[Bauer CM]] with [[Marte V]], [[Laner V]]<br />
* [[List_of_all_products|OROBOROS products]]: [[Fasching M]] with [[Marte V]]<br />
* [[MitoCom]]: [[Laner V]]<br />
* [[IOC]]: [[Marte V]] with [[Bauer CM]], [[Laner V]]<br />
* [[Troubleshooting]]: [[Fasching M]] with [[Fontana-Ayoub M]], [[Heidler J]]<br />
<br />
=== Bioblast scientific editors ===<br />
<br />
* [[Doerrier C]], ES (2011)<br />
* [[Fasching M]], AT (2010)<br />
* [[Gnaiger E]], AT (2010)<br />
* [[Goncalves RL]], BR (presently US) (2012)<br />
* [[Harrison DK]], AT (IT) (2011)<br />
* [[Krumschnabel G]], AT (2013)<br />
* [[Pesta D]], AT (presently US) (2011)<br />
<br />
<br />
<br />
= Bioblast editorial strategies =<br />
<br />
<br />
== Why a wiki editorial board? ==<br />
<br />
We intend to provide the Bioblast wiki as an open forum and 'information synthase' in the field of mitochondrial physiology. It took a few months until this wiki was discovered by the SPAM distributors, and free access to generate a personal user account had to be discontinued. Apart from attacks by the unfriendly abuse of the open wiki, we need to develop a transparent strategy for securing our aim at a scientifically based information website. Thus we invite colleagues from all-over-the world to contact us for creating a personal account, and to join us as members of the editorial board. --[[User:Gnaiger Erich|Gnaiger Erich]] 17:19, 24 December 2011 (CET)<br />
<br />
'''* A scientific strategy in the spirit of Open Access and Open Innovation: [[Bioblast-MitoPedia]]'''<br />
<br />
<br />
== Personal data published in the Bioblast wiki ==<br />
<br />
Personal data are published in the Bioblast wiki, e.g. in the context of abstracts or references, in the form of professional addresses and Email addresses. This information is helpful for networking. In general, such information is published in the Bioblast website with the consent of anybody involved. We appreciate any instruction on data (e.g. Email address) that should be corrected or should not be published.<br />
<br />
== Bioblast editorial tasks ==<br />
=== General ===<br />
Bioblast editors may have broad or highly focussed tasks:<br />
:* Maintain the structure of Bioblast ([[Bioblast_editorial_board#Bioblast_advisory_board|Bioblast advisory board]]).<br />
:* Take responsibility for specific sections in Bioblast ([[Bioblast_editorial_board#Bioblast_editorial_board_-_section_editors|Bioblast section editors]]).<br />
:* Add and edit various articles and references ([[Bioblast_editorial_board#Bioblast_editorial_board_-_scientific_editors|Bioblast scientific editors]] and active Bioblast users).<br />
:* Prepare '''[[Bioblast alert]]''' messages to draw attention to specific topics.<br />
<br />
=== Tasks of Bioblast national editors ===<br />
:* Communicate regularly with [[MiPNet Reference Laboratories]] to obtain feedback on the Bioblast wiki.<br />
:* See [[Draft letter to MiPNet Reference Laboratories]]<br />
:* Ask them regularly about updates on their [[O2k-Publications]] and complete the list of publications.<br />
:* Help them to update their Bioblast page.<br />
:* Perhaps introduce a specific member of the lab to actively participate in updating their page.<br />
:* Communicate with the advisory board about problems and structural improvements of the Bioblast wiki.<br />
<br />
== How do you become a member of the Bioblast editorial board? ==<br />
Scientists and students of mitochondrial physiology are invited to join the '''Bioblast editorial board''', to help us maintaining a high and up-to-date scientific standard of the [[Bioblast:About|Bioblast wiki]]. Please, send a message to [[Bauer CM|Christiane Bauer]] for receiving your personal active user account, and to [[Gnaiger E|Erich Gnaiger]] to join as a Scientific Editor of the [[Bioblast_editorial_board#Bioblast_scientific_editors|Bioblast Editorial Board]].<br />
<br />
= Editorial help =<br />
<br />
:* [[Bioblast-Icons]] <br />
:* [[Bioblast manual]]<br />
:* [[Help:Contents]]<br />
:* [[Bioblast-MitoPedia]]</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MiP2014&diff=58203MiP20142014-03-26T12:59:57Z<p>Bauer Christiane: /* MiP2014 preliminary participants */</p>
<hr />
<div>[[Image:IUBMB-logo.gif|left|100px|link=http://www.iubmb.org|IUBMB - ''International Union of Biochemistry and Molecular Biology'']]<br />
[[Image:MiPsocietyLOGO.JPG|right|200px|link=http://www.mitophysiology.org|MiPsociety]]<br />
[http://www.mitophysiology.org/?MiP2014 '''<big>MiP2014 - 10<sup>th</sup> MiP''conference'': Joint IUBMB/MiP Symposium on Mitochondrial Physiology - a Point/Counterpoint Meeting</big>''']<br />
<br />
'''''International Union of Biochemistry and Molecular Biology''''' and '''Mitochondrial Physiology Society'''<br />
<br />
Obergurgl, Tyrol, Austria. 2014-Sep-08 to 12<br />
== MiP2014 - a point/counterpoint meeting ==<br />
<br />
Mitochondrial research has recently undergone a dramatic renaissance, due to numerous discoveries revealing its central role in cell death, disease pathology, aging, thermogenesis, oxidative stress, cell signalling, cellular regulation and cell bioenergetics. The aim of the small symposium is to bring together experts in mitochondrial research spanning from the biochemical and molecular level to the interactions within the cell. In addition to the latest scientific findings, new methodological developments in measuring mitochondrial function and dysfunction are presented. A point/counterpoint format is chosen to discuss selected hot topics and some fundamental methodological and conceptual issues.<br />
<br />
The expected attendance is max. 100 participants. ->> Compare [[MiP2013 Programme]]<br />
Abstracts and presentations: refer to [http://www.chem.qmul.ac.uk/iubmb/ IUBMB nomenclature]; >> [[MitoPedia Glossary: Respiratory states|MitoPedia Glossary: '''Respiratory states''']]<br />
<br />
== Programme structure ==<br />
<br />
In each of the 12 sessions a point/counterpoint introduction will be given by invited keynote speakers to provide a balanced overview and promote discussions. Short oral presentations are selected from abstracts, including student presentations. Rapid platform statements will introduce posters which contribute to a specific session. Posters are up throughout the meeting, and poster discussions are integrated into the platform sessions.<br />
<br />
=== A. Mitochondrial respiratory control === <br />
* A1. mtDNA, mt-phenotype and disease – is there biochemical and molecular-genetic evidence to explain observed associations? <br />
* A2. Fission and fusion, mitochondrial structure and function - is there a tight connection? <br />
* A3. Mitochondrial density and normalization of respiration – is there a general biochemical or molecular marker? <br />
* A4. Biochemical reserve capacity, respiratory control ratios and coupling efficiency – are conventional concepts inconsistent?<br />
<br />
=== B. Experimental protocols in mitochondrial biochemistry === <br />
* B1. Incubation media for mt-preparations and experimental temperature in functional studies of mammalian mitochondria – guided by concept or tradition? <br />
* B2. Intracellular oxygen pressure versus oxygen regimes in studies of ROS production and respiration - are mitochondria sources or sinks of ROS?<br />
* B3. Quantitative measurement of mt-membrane potential - potentiometric/fluorometric. <br />
* B4. Instrumental platforms in mitochondrial physiology – industrial presentations.<br />
<br />
=== C. Towards quality assurance === <br />
* C1. Do blood cells versus muscle biopsies provide valid models for diagnosis of mitochondrial pathologies? <br />
* C2. Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
* C3. From availability and quality control of chemicals to mitochondrial respiratory protocols – who controls? <br />
* C4. Standards for the diagnosis of mitochondrial pathologies – a challenge for collaboration with mitochondrial organizations?<br />
Particularly in view of Subtopic C, it may be attractive for mitochondrial organizations to send and support delegates. The conference will, therefore, provide a unique forum for international networking, and may inspire new national groups to be formed in contact with established mitochondrial organizations:<br />
>> '''[[MitoGlobal_Organizations]]'''<br />
<br />
== MiP2014 preliminary participants ==<br />
<br />
in progress<br />
<br />
{| class="wikitable"<br />
|-<br />
! Name !! Institution !! Preliminary title <br />
|-<br />
| [[Ali SS|Ali Sameh S]]||Zewail City for Science and Technology, ET||Oxygen-consuming and ROS-producing activities in synaptosomes.<br />
|-<br />
| [[Bauer CM|Bauer Christiane Maria]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Borutaite_V|Borutaite Vilmante]]||Lithuanian University of Health Sciences, Kaunas, LT||Controversies in testing integrity of mitochondrial membranes.<br />
|-<br />
| [[Brown D|Brown David]]||East Carolina University, NC, US||<br />
|-<br />
| [[Contreras-Ferrat A|Contreras-Ferrat Ariel]]||University of Chile, CL||<br />
|-<br />
| [[Coen P|Coen Paul]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Dohlmann T|Dohlmann Tine]]||University of Copenhagen, DK||<br />
|-<br />
| [[Elmer E|Elmer Eskil]]||Wallenberg Neuroscience Center, Lund, SE||Ex vivo respiratory capacity of human blood cells - a SUITable application of the O2k Oxygraph / A novel pharmacological approach to counteract metabolic crisis.<br />
|-<br />
| [[Felding-Habermann B|Felding-Habermann Brunhilde]]||The Scripps Research Institute, La Jolla, CA, US||Mitochondrial functions in breast cancer progression.<br />
|-<br />
| [[Goodpaster BH|Goodpaster Bret]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Gnaiger E|Gnaiger Erich]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Grefte S|Grefte Sander]]||Radboud University Nijmegen Medical Centre, Nijmegen, NL||Pathophysiology of mitochondrial (dys)function in skeletal muscle.<br />
|-<br />
| [[Hahn D|Hahn Dagmar]]||Inselspital Bern, CH||<br />
|-<br />
| [[Hand SC|Hand Steven C]]||Louisiana State University, LA, US||Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
|-<br />
| [[Harrison DK|Harrison David K]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Hepple_RT| Hepple Russel T.]]||McGill University, Montreal, CA||Mitochondrial involvement in aging muscle: cause and effect(or).<br />
|-<br />
| [[Hey-Mogensen M|Hey-Mogensen Martin]]||Novo Nordisk A/S Diabetes NBEs & Obesity Biology, Måløv, DK||<br />
|-<br />
| [[Jana Prado F|Jana Prado Fabian]]||University of Chile, CL||<br />
|-<br />
| [[Kane DA| Kane Daniel A.]]||St. Francis Xavier University, Antigonish, CA||Mitochondrial shuttles in muscle.<br />
|-<br />
| [[Kraunsoe R|Kraunsoe Regitze]]||University of Copenhagen, DK||<br />
|-<br />
| [[Koopman WJ|Koopman Werner J]]||Radboud University Nijmegen Medical Centre, NL||Redox regulation of mitochondrial dynamics.<br />
|-<br />
| [[Kowaltowski AJ|Kowaltowski Alicia J.]]||University of Sao Paulo, BR||<br />
|-<br />
| [[Laner V|Laner Verena]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Larsen S|Larsen Steen]]||University of Copenhagen, DK||Normalization of respiration.<br />
|-<br />
| [[Larsson NG|Göran-Larsson Nils]]||Max Planck Institute for the Biology of Ageing, Cologne, DE||Mouse models with mtDNA mutations.<br />
|-<br />
| [[Ojuka E|Ojuka Edward]]||University of Cape Town, ZA||Fission and fusion, mitochondrial structure and function - is there a tight connection?<br />
|-<br />
| [[Perry CG|Perry Christopher G]]||York University, Ontario, CA||Re-visiting critical experimental parameters to control when measuring respiratory kinetics in permeabilized muscle fibres.<br />
|-<br />
| [[Pesta D|Pesta Dominik]]||Yale University School of Medicine, CT, US||<br />
|- <br />
| [[Picard_M|Picard Martin]]||University of Pennsylvania, PA, US||Quantitative regulation of nuclear gene expression by mitochondrial DNA<br />
heteroplasmy.<br />
|-<br />
| [[Pecina P|Pecina Petr]]||Academy of Sciences of the Czech Republic, Prague, CZ||The use of lymphocytes for diagnostics of mitochondrial disorders.<br />
|-<br />
| [[Purhonen J|Purhonen Janne]]||University Helsinki, FI||<br />
|-<br />
| [[Ristow M|Ristow Michael]]||ETH Zurich, CH||Promoting health and lifespan by increasing mitochondrial ROS formation.<br />
|-<br />
| [[Shirihai O|Shirihai Orian]]||The Shirihai Lab at Boston University, MA, US||<br />
|-<br />
| [[Skulachev VP|Skulachev Vladimir]]||Lomonosov Moscow State University, Moscow, RU||Mitochondria are sources of ROS rather than sinks: Physiological effects of mitochondria-targeted antioxidants.<br />
|-<br />
| [[Starkov AA|Starkov Anatoly]]||Weill Medical College Cornell University, NY, US||<br />
|-<br />
| [[Stiban J|Stiban Johnny]]||Birzeit University, Ramallah, PS||Very long chain ceramides interfere with C16-ceramide-induced channel formation: A plausible mechanism for regulating the initiation of intrinsic apoptosis.<br />
|-<br />
| [[Zorzano A|Zorzano Antonio]]||Universitat de Barcelona, ES||Metabolic implications of mitofusin 2 dysfunction.<br />
|-<br />
| [[Sumbalova Z|Sumbalova Zuzana]]||Comenius University, Bratislava, SK||<br />
|}<br />
<br />
== Venue ==<br />
<br />
The symposium will be hosted in the '''University Center in Obergurgl''' 100 km from Innsbruck. The closest airport is in Innsbruck. Participants arriving at Munich airport will take the train or an airport shuttle to Innsbruck. We provide support in arranging the transfer between Munich and Innsbruck. A bus transfer is organized between Innsbruck and Obergurgl.<br />
<br />
The Obergurgl University Center is located at an altitude of 1940 meters above sea-level. The historical buildings, carefully refurbished and equipped with the latest technical systems, can accommodate around 70 persons in 43 bedrooms and includes conference rooms, the Alpine Research Center, laboratory, sports medicine, lounges and the restaurant. All rooms are equipped with an Internet connection. Additional participants are accommodated in smaller guest houses in 5 minutes walking distance. Meals are served for all participants in the University Center. Two conference rooms for a total of 200 persons and three small rooms for round table discussions are available. There is enough space for poster presentations and instrument exhibitions. The cosy bar and lounge area with an open fire place adds to the familiar atmosphere and supports the interactive spirit of the symposium.<br />
<br />
The small village of Obergurgl is situated in an impressive landscape at about 1900 m above sea level in the Ötz valley. It is surrounded by mountains, offering an exceptional view to the Ötz valley glaciers. During summer time the area is famous for hiking and for skiing in winter time. On the doorstep: one of Europe´s finest Biosphere Parks. The unique surroundings offer the possibility of combining scientific discussions while exploring the alpine environment during the famous “walks and talks”. The informal atmosphere of the workshop encourages also students and young post-docs to participate actively in scientific discussions and to develop future collaborations.<br />
<br />
University Centre Obergurgl, Gaisbergweg 5, 6456 Obergurgl, Tyrol, Austria.<br />
Tel.: +43 (0) 512 507 37201 | Fax: +43 (0) 512 507 37400 | Email: obergurgl@uibk.ac.at <br />
<br />
== International programme committee ==<br />
The international programme committee will provide scientific input by active participation, nominate further invited speakers to ensure balanced point/counterpoint introductions, and select the abstracts as a basis for participation (restricted number of participants), for oral 10+5 min presentations, and for flash presentations.<br />
<br />
Chair:<br />
* [[Gnaiger E|Gnaiger Erich]], Medical University Innsbruck, AT. - erich.gnaiger@mitophysiology.org<br />
Members:<br />
* [[Ali SS|Ali Sameh S]], Helmy Institute of Medical Sciences, Giza, Egypt<br />
* [[Borutaite V|Borutaite Vilma]], Lithuanian University of Health Sciences Kaunas, LT<br />
* [[Neurocon|Chakrabarti Sasanka]], Institute of Post-graduate Medical Education & Research, Kolkatta, India<br />
* [[Chinese Society of Mitochondrial Research and Medicine|Chen Quan]], Chinese Academy of Sciences, Nankai University, Beijing <br />
* [[Dela F|Dela Flemming]], University of Copenhagen, DK <br />
* [[Duchen MR|Duchen Michael R]], University College London, UK<br />
* [[Elmer E|Elmér Eskil]], Lund University, SE <br />
* [[Hand SC|Hand Steven]], Louisiana State University, LA, US<br />
* [[Hickey AJ|Hickey Anthony J]], University of Auckland, NZ<br />
* [[Koopman WJ|Koopman Werner J]], Radboud Univ Nijmegen Medical Centre, NL<br />
* [[Tanaka M|Tanaka Masashi]], Toyko Metropolitan Institute of Gerontology, JP<br />
* [[Neufer PD|Neufer P Darrell]], East Carolina University, Greenville, NC, US<br />
* [[Ojuka E|Ojuka Edward]], University of Cape Town, South Africa<br />
* [[Pecina P|Pecina Petr]], Academy of Sciences of the Czech Republic, CZ<br />
* [[Perry CG|Perry Christopher G]], York University, Toronto, Canada<br />
* [[Rossignol R|Rossignol Rodrigue]], Université Victor Segalen-Bordeaux, FR<br />
* [[Vercesi AE|Vercesi Anibal E]], State University of Campinas, Sao Paulo, Brazil<br />
* [[Taiwan Society for Mitochondrial Research and Medicine|Wei Yau-Huei]], Mackay Medical College, New Taipei City, Taiwan<br />
* [[Zorzano A|Zorzano Antonio]], Universitat de Barcelona, Spain<br />
<br />
== Information for invited speakers==<br />
<br />
MiP2014 will be held at the University Centre Obergurgl, with maximum capacity of 124 persons and a price per person per night of € 65.- including a shared double room, breakfast, and dinner. MiP''conferences'' are organized as small meetings facilitating communication between senior and junior participants. In the tradition of MiP''conferences'', we are asking invited speakers to cooperate and try to receive local support for joining us at the prestigious Mitochondrial Physiology Conference (limited to 124 participants). Support will be provided to students and young investigators.<br />
<br />
== Post-conference O2k-Workshop ==<br />
<br />
A post-conference workshop is organized on the same venue, for hands-on training in mitochondrial respiratory physiology, introducing the combined measurement of high-resolution respirometry and fluorometry (OROBOROS INSTRUMENTS). Since participants come from all-over-the-world, including many students, the combination of the symposium with the workshop makes travelling more economical for the maximum of 24 workshop-participants. The post-conference workshop is financially completely separate from the symposium.<br />
<br />
>> '''[[MitoGlobal_Events_2014]]'''<br />
<br />
<br />
<br />
<br />
<br />
[[Category:MitoPedia]]<br />
[[Category:All]]</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=NL_Nijmegen_Koopman_WJH&diff=58202NL Nijmegen Koopman WJH2014-03-26T12:54:47Z<p>Bauer Christiane: </p>
<hr />
<div>{{MiPNetLab<br />
|institution=Dept. of Biochemistry (286) <br />
<br />
Nijmegen Centre for Molecular Life Sciences<br />
<br />
Radboud University Nijmegen Medical Centre<br />
|city=Nijmegen<br />
|country=Netherlands<br />
|Contact=Koopman WJ<br />
|MiPNetLab=Forkink M, Manjeri G, Wagenaars J, Grefte S<br />
|Status=O2k 2010-<br />
|info=[[IOC59]]<br />
}}</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=Grefte_Sander&diff=58201Grefte Sander2014-03-26T12:53:49Z<p>Bauer Christiane: </p>
<hr />
<div>{{Person<br />
|lastname=Grefte<br />
|firstname=Sander<br />
|title=PhD<br />
}}<br />
{{Labelingperson}}</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=Grefte_Sander&diff=58200Grefte Sander2014-03-26T12:53:30Z<p>Bauer Christiane: Created page with "{{Person |firstname=Sander |lastname=Grefte }}"</p>
<hr />
<div>{{Person<br />
|firstname=Sander<br />
|lastname=Grefte<br />
}}</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MiP2014&diff=58199MiP20142014-03-26T11:21:19Z<p>Bauer Christiane: /* MiP2014 preliminary participants */</p>
<hr />
<div>[[Image:IUBMB-logo.gif|left|100px|link=http://www.iubmb.org|IUBMB - ''International Union of Biochemistry and Molecular Biology'']]<br />
[[Image:MiPsocietyLOGO.JPG|right|200px|link=http://www.mitophysiology.org|MiPsociety]]<br />
[http://www.mitophysiology.org/?MiP2014 '''<big>MiP2014 - 10<sup>th</sup> MiP''conference'': Joint IUBMB/MiP Symposium on Mitochondrial Physiology - a Point/Counterpoint Meeting</big>''']<br />
<br />
'''''International Union of Biochemistry and Molecular Biology''''' and '''Mitochondrial Physiology Society'''<br />
<br />
Obergurgl, Tyrol, Austria. 2014-Sep-08 to 12<br />
== MiP2014 - a point/counterpoint meeting ==<br />
<br />
Mitochondrial research has recently undergone a dramatic renaissance, due to numerous discoveries revealing its central role in cell death, disease pathology, aging, thermogenesis, oxidative stress, cell signalling, cellular regulation and cell bioenergetics. The aim of the small symposium is to bring together experts in mitochondrial research spanning from the biochemical and molecular level to the interactions within the cell. In addition to the latest scientific findings, new methodological developments in measuring mitochondrial function and dysfunction are presented. A point/counterpoint format is chosen to discuss selected hot topics and some fundamental methodological and conceptual issues.<br />
<br />
The expected attendance is max. 100 participants. ->> Compare [[MiP2013 Programme]]<br />
Abstracts and presentations: refer to [http://www.chem.qmul.ac.uk/iubmb/ IUBMB nomenclature]; >> [[MitoPedia Glossary: Respiratory states|MitoPedia Glossary: '''Respiratory states''']]<br />
<br />
== Programme structure ==<br />
<br />
In each of the 12 sessions a point/counterpoint introduction will be given by invited keynote speakers to provide a balanced overview and promote discussions. Short oral presentations are selected from abstracts, including student presentations. Rapid platform statements will introduce posters which contribute to a specific session. Posters are up throughout the meeting, and poster discussions are integrated into the platform sessions.<br />
<br />
=== A. Mitochondrial respiratory control === <br />
* A1. mtDNA, mt-phenotype and disease – is there biochemical and molecular-genetic evidence to explain observed associations? <br />
* A2. Fission and fusion, mitochondrial structure and function - is there a tight connection? <br />
* A3. Mitochondrial density and normalization of respiration – is there a general biochemical or molecular marker? <br />
* A4. Biochemical reserve capacity, respiratory control ratios and coupling efficiency – are conventional concepts inconsistent?<br />
<br />
=== B. Experimental protocols in mitochondrial biochemistry === <br />
* B1. Incubation media for mt-preparations and experimental temperature in functional studies of mammalian mitochondria – guided by concept or tradition? <br />
* B2. Intracellular oxygen pressure versus oxygen regimes in studies of ROS production and respiration - are mitochondria sources or sinks of ROS?<br />
* B3. Quantitative measurement of mt-membrane potential - potentiometric/fluorometric. <br />
* B4. Instrumental platforms in mitochondrial physiology – industrial presentations.<br />
<br />
=== C. Towards quality assurance === <br />
* C1. Do blood cells versus muscle biopsies provide valid models for diagnosis of mitochondrial pathologies? <br />
* C2. Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
* C3. From availability and quality control of chemicals to mitochondrial respiratory protocols – who controls? <br />
* C4. Standards for the diagnosis of mitochondrial pathologies – a challenge for collaboration with mitochondrial organizations?<br />
Particularly in view of Subtopic C, it may be attractive for mitochondrial organizations to send and support delegates. The conference will, therefore, provide a unique forum for international networking, and may inspire new national groups to be formed in contact with established mitochondrial organizations:<br />
>> '''[[MitoGlobal_Organizations]]'''<br />
<br />
== MiP2014 preliminary participants ==<br />
<br />
in progress<br />
<br />
{| class="wikitable"<br />
|-<br />
! Name !! Institution !! Preliminary title <br />
|-<br />
| [[Ali SS|Ali Sameh S]]||Zewail City for Science and Technology, ET||Oxygen-consuming and ROS-producing activities in synaptosomes.<br />
|-<br />
| [[Bauer CM|Bauer Christiane Maria]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Borutaite_V|Borutaite Vilmante]]||Lithuanian University of Health Sciences, Kaunas, LT||Controversies in testing integrity of mitochondrial membranes.<br />
|-<br />
| [[Brown D|Brown David]]||East Carolina University, NC, US||<br />
|-<br />
| [[Contreras-Ferrat A|Contreras-Ferrat Ariel]]||University of Chile, CL||<br />
|-<br />
| [[Coen P|Coen Paul]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Dohlmann T|Dohlmann Tine]]||University of Copenhagen, DK||<br />
|-<br />
| [[Elmer E|Elmer Eskil]]||Wallenberg Neuroscience Center, Lund, SE||Ex vivo respiratory capacity of human blood cells - a SUITable application of the O2k Oxygraph / A novel pharmacological approach to counteract metabolic crisis.<br />
|-<br />
| [[Felding-Habermann B|Felding-Habermann Brunhilde]]||The Scripps Research Institute, La Jolla, CA, US||Mitochondrial functions in breast cancer progression.<br />
|-<br />
| [[Goodpaster BH|Goodpaster Bret]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Gnaiger E|Gnaiger Erich]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Hahn D|Hahn Dagmar]]||Inselspital Bern, CH||<br />
|-<br />
| [[Hand SC|Hand Steven C]]||Louisiana State University, LA, US||Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
|-<br />
| [[Harrison DK|Harrison David K]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Hepple_RT| Hepple Russel T.]]||McGill University, Montreal, CA||Mitochondrial involvement in aging muscle: cause and effect(or).<br />
|-<br />
| [[Hey-Mogensen M|Hey-Mogensen Martin]]||Novo Nordisk A/S Diabetes NBEs & Obesity Biology, Måløv, DK||<br />
|-<br />
| [[Jana Prado F|Jana Prado Fabian]]||University of Chile, CL||<br />
|-<br />
| [[Kane DA| Kane Daniel A.]]||St. Francis Xavier University, Antigonish, CA||Mitochondrial shuttles in muscle.<br />
|-<br />
| [[Kraunsoe R|Kraunsoe Regitze]]||University of Copenhagen, DK||<br />
|-<br />
| [[Koopman WJ|Koopman Werner J]]||Radboud University Nijmegen Medical Centre, NL||Redox regulation of mitochondrial dynamics.<br />
|-<br />
| [[Kowaltowski AJ|Kowaltowski Alicia J.]]||University of Sao Paulo, BR||<br />
|-<br />
| [[Laner V|Laner Verena]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Larsen S|Larsen Steen]]||University of Copenhagen, DK||Normalization of respiration.<br />
|-<br />
| [[Larsson NG|Göran-Larsson Nils]]||Max Planck Institute for the Biology of Ageing, Cologne, DE||Mouse models with mtDNA mutations.<br />
|-<br />
| [[Ojuka E|Ojuka Edward]]||University of Cape Town, ZA||Fission and fusion, mitochondrial structure and function - is there a tight connection?<br />
|-<br />
| [[Perry CG|Perry Christopher G]]||York University, Ontario, CA||Re-visiting critical experimental parameters to control when measuring respiratory kinetics in permeabilized muscle fibres.<br />
|-<br />
| [[Pesta D|Pesta Dominik]]||Yale University School of Medicine, CT, US||<br />
|- <br />
| [[Picard_M|Picard Martin]]||University of Pennsylvania, PA, US||Quantitative regulation of nuclear gene expression by mitochondrial DNA<br />
heteroplasmy.<br />
|-<br />
| [[Pecina P|Pecina Petr]]||Academy of Sciences of the Czech Republic, Prague, CZ||The use of lymphocytes for diagnostics of mitochondrial disorders.<br />
|-<br />
| [[Purhonen J|Purhonen Janne]]||University Helsinki, FI||<br />
|-<br />
| [[Ristow M|Ristow Michael]]||ETH Zurich, CH||Promoting health and lifespan by increasing mitochondrial ROS formation.<br />
|-<br />
| [[Shirihai O|Shirihai Orian]]||The Shirihai Lab at Boston University, MA, US||<br />
|-<br />
| [[Skulachev VP|Skulachev Vladimir]]||Lomonosov Moscow State University, Moscow, RU||Mitochondria are sources of ROS rather than sinks: Physiological effects of mitochondria-targeted antioxidants.<br />
|-<br />
| [[Starkov AA|Starkov Anatoly]]||Weill Medical College Cornell University, NY, US||<br />
|-<br />
| [[Stiban J|Stiban Johnny]]||Birzeit University, Ramallah, PS||Very long chain ceramides interfere with C16-ceramide-induced channel formation: A plausible mechanism for regulating the initiation of intrinsic apoptosis.<br />
|-<br />
| [[Zorzano A|Zorzano Antonio]]||Universitat de Barcelona, ES||Metabolic implications of mitofusin 2 dysfunction.<br />
|-<br />
| [[Sumbalova Z|Sumbalova Zuzana]]||Comenius University, Bratislava, SK||<br />
|}<br />
<br />
== Venue ==<br />
<br />
The symposium will be hosted in the '''University Center in Obergurgl''' 100 km from Innsbruck. The closest airport is in Innsbruck. Participants arriving at Munich airport will take the train or an airport shuttle to Innsbruck. We provide support in arranging the transfer between Munich and Innsbruck. A bus transfer is organized between Innsbruck and Obergurgl.<br />
<br />
The Obergurgl University Center is located at an altitude of 1940 meters above sea-level. The historical buildings, carefully refurbished and equipped with the latest technical systems, can accommodate around 70 persons in 43 bedrooms and includes conference rooms, the Alpine Research Center, laboratory, sports medicine, lounges and the restaurant. All rooms are equipped with an Internet connection. Additional participants are accommodated in smaller guest houses in 5 minutes walking distance. Meals are served for all participants in the University Center. Two conference rooms for a total of 200 persons and three small rooms for round table discussions are available. There is enough space for poster presentations and instrument exhibitions. The cosy bar and lounge area with an open fire place adds to the familiar atmosphere and supports the interactive spirit of the symposium.<br />
<br />
The small village of Obergurgl is situated in an impressive landscape at about 1900 m above sea level in the Ötz valley. It is surrounded by mountains, offering an exceptional view to the Ötz valley glaciers. During summer time the area is famous for hiking and for skiing in winter time. On the doorstep: one of Europe´s finest Biosphere Parks. The unique surroundings offer the possibility of combining scientific discussions while exploring the alpine environment during the famous “walks and talks”. The informal atmosphere of the workshop encourages also students and young post-docs to participate actively in scientific discussions and to develop future collaborations.<br />
<br />
University Centre Obergurgl, Gaisbergweg 5, 6456 Obergurgl, Tyrol, Austria.<br />
Tel.: +43 (0) 512 507 37201 | Fax: +43 (0) 512 507 37400 | Email: obergurgl@uibk.ac.at <br />
<br />
== International programme committee ==<br />
The international programme committee will provide scientific input by active participation, nominate further invited speakers to ensure balanced point/counterpoint introductions, and select the abstracts as a basis for participation (restricted number of participants), for oral 10+5 min presentations, and for flash presentations.<br />
<br />
Chair:<br />
* [[Gnaiger E|Gnaiger Erich]], Medical University Innsbruck, AT. - erich.gnaiger@mitophysiology.org<br />
Members:<br />
* [[Ali SS|Ali Sameh S]], Helmy Institute of Medical Sciences, Giza, Egypt<br />
* [[Borutaite V|Borutaite Vilma]], Lithuanian University of Health Sciences Kaunas, LT<br />
* [[Neurocon|Chakrabarti Sasanka]], Institute of Post-graduate Medical Education & Research, Kolkatta, India<br />
* [[Chinese Society of Mitochondrial Research and Medicine|Chen Quan]], Chinese Academy of Sciences, Nankai University, Beijing <br />
* [[Dela F|Dela Flemming]], University of Copenhagen, DK <br />
* [[Duchen MR|Duchen Michael R]], University College London, UK<br />
* [[Elmer E|Elmér Eskil]], Lund University, SE <br />
* [[Hand SC|Hand Steven]], Louisiana State University, LA, US<br />
* [[Hickey AJ|Hickey Anthony J]], University of Auckland, NZ<br />
* [[Koopman WJ|Koopman Werner J]], Radboud Univ Nijmegen Medical Centre, NL<br />
* [[Tanaka M|Tanaka Masashi]], Toyko Metropolitan Institute of Gerontology, JP<br />
* [[Neufer PD|Neufer P Darrell]], East Carolina University, Greenville, NC, US<br />
* [[Ojuka E|Ojuka Edward]], University of Cape Town, South Africa<br />
* [[Pecina P|Pecina Petr]], Academy of Sciences of the Czech Republic, CZ<br />
* [[Perry CG|Perry Christopher G]], York University, Toronto, Canada<br />
* [[Rossignol R|Rossignol Rodrigue]], Université Victor Segalen-Bordeaux, FR<br />
* [[Vercesi AE|Vercesi Anibal E]], State University of Campinas, Sao Paulo, Brazil<br />
* [[Taiwan Society for Mitochondrial Research and Medicine|Wei Yau-Huei]], Mackay Medical College, New Taipei City, Taiwan<br />
* [[Zorzano A|Zorzano Antonio]], Universitat de Barcelona, Spain<br />
<br />
== Information for invited speakers==<br />
<br />
MiP2014 will be held at the University Centre Obergurgl, with maximum capacity of 124 persons and a price per person per night of € 65.- including a shared double room, breakfast, and dinner. MiP''conferences'' are organized as small meetings facilitating communication between senior and junior participants. In the tradition of MiP''conferences'', we are asking invited speakers to cooperate and try to receive local support for joining us at the prestigious Mitochondrial Physiology Conference (limited to 124 participants). Support will be provided to students and young investigators.<br />
<br />
== Post-conference O2k-Workshop ==<br />
<br />
A post-conference workshop is organized on the same venue, for hands-on training in mitochondrial respiratory physiology, introducing the combined measurement of high-resolution respirometry and fluorometry (OROBOROS INSTRUMENTS). Since participants come from all-over-the-world, including many students, the combination of the symposium with the workshop makes travelling more economical for the maximum of 24 workshop-participants. The post-conference workshop is financially completely separate from the symposium.<br />
<br />
>> '''[[MitoGlobal_Events_2014]]'''<br />
<br />
<br />
<br />
<br />
<br />
[[Category:MitoPedia]]<br />
[[Category:All]]</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MiP2014&diff=58196MiP20142014-03-26T11:14:59Z<p>Bauer Christiane: /* MiP2014 preliminary participants */</p>
<hr />
<div>[[Image:IUBMB-logo.gif|left|100px|link=http://www.iubmb.org|IUBMB - ''International Union of Biochemistry and Molecular Biology'']]<br />
[[Image:MiPsocietyLOGO.JPG|right|200px|link=http://www.mitophysiology.org|MiPsociety]]<br />
[http://www.mitophysiology.org/?MiP2014 '''<big>MiP2014 - 10<sup>th</sup> MiP''conference'': Joint IUBMB/MiP Symposium on Mitochondrial Physiology - a Point/Counterpoint Meeting</big>''']<br />
<br />
'''''International Union of Biochemistry and Molecular Biology''''' and '''Mitochondrial Physiology Society'''<br />
<br />
Obergurgl, Tyrol, Austria. 2014-Sep-08 to 12<br />
== MiP2014 - a point/counterpoint meeting ==<br />
<br />
Mitochondrial research has recently undergone a dramatic renaissance, due to numerous discoveries revealing its central role in cell death, disease pathology, aging, thermogenesis, oxidative stress, cell signalling, cellular regulation and cell bioenergetics. The aim of the small symposium is to bring together experts in mitochondrial research spanning from the biochemical and molecular level to the interactions within the cell. In addition to the latest scientific findings, new methodological developments in measuring mitochondrial function and dysfunction are presented. A point/counterpoint format is chosen to discuss selected hot topics and some fundamental methodological and conceptual issues.<br />
<br />
The expected attendance is max. 100 participants. ->> Compare [[MiP2013 Programme]]<br />
Abstracts and presentations: refer to [http://www.chem.qmul.ac.uk/iubmb/ IUBMB nomenclature]; >> [[MitoPedia Glossary: Respiratory states|MitoPedia Glossary: '''Respiratory states''']]<br />
<br />
== Programme structure ==<br />
<br />
In each of the 12 sessions a point/counterpoint introduction will be given by invited keynote speakers to provide a balanced overview and promote discussions. Short oral presentations are selected from abstracts, including student presentations. Rapid platform statements will introduce posters which contribute to a specific session. Posters are up throughout the meeting, and poster discussions are integrated into the platform sessions.<br />
<br />
=== A. Mitochondrial respiratory control === <br />
* A1. mtDNA, mt-phenotype and disease – is there biochemical and molecular-genetic evidence to explain observed associations? <br />
* A2. Fission and fusion, mitochondrial structure and function - is there a tight connection? <br />
* A3. Mitochondrial density and normalization of respiration – is there a general biochemical or molecular marker? <br />
* A4. Biochemical reserve capacity, respiratory control ratios and coupling efficiency – are conventional concepts inconsistent?<br />
<br />
=== B. Experimental protocols in mitochondrial biochemistry === <br />
* B1. Incubation media for mt-preparations and experimental temperature in functional studies of mammalian mitochondria – guided by concept or tradition? <br />
* B2. Intracellular oxygen pressure versus oxygen regimes in studies of ROS production and respiration - are mitochondria sources or sinks of ROS?<br />
* B3. Quantitative measurement of mt-membrane potential - potentiometric/fluorometric. <br />
* B4. Instrumental platforms in mitochondrial physiology – industrial presentations.<br />
<br />
=== C. Towards quality assurance === <br />
* C1. Do blood cells versus muscle biopsies provide valid models for diagnosis of mitochondrial pathologies? <br />
* C2. Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
* C3. From availability and quality control of chemicals to mitochondrial respiratory protocols – who controls? <br />
* C4. Standards for the diagnosis of mitochondrial pathologies – a challenge for collaboration with mitochondrial organizations?<br />
Particularly in view of Subtopic C, it may be attractive for mitochondrial organizations to send and support delegates. The conference will, therefore, provide a unique forum for international networking, and may inspire new national groups to be formed in contact with established mitochondrial organizations:<br />
>> '''[[MitoGlobal_Organizations]]'''<br />
<br />
== MiP2014 preliminary participants ==<br />
<br />
in progress<br />
<br />
{| class="wikitable"<br />
|-<br />
! Name !! Institution !! Preliminary title <br />
|-<br />
| [[Ali SS|Ali Sameh S]]||Zewail City for Science and Technology, ET||Oxygen-consuming and ROS-producing activities in synaptosomes.<br />
|-<br />
| [[Bauer CM|Bauer Christiane Maria]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Borutaite_V|Borutaite Vilmante]]||Lithuanian University of Health Sciences, Kaunas, LT||Controversies in testing integrity of mitochondrial membranes.<br />
|-<br />
| [[Brown D|Brown David]]||East Carolina University, NC, US||<br />
|-<br />
| [[Contreras-Ferrat A|Contreras-Ferrat Ariel]]||University of Chile, CL||<br />
|-<br />
| [[Coen P|Coen Paul]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Dohlmann T|Dohlmann Tine]]||University of Copenhagen, DK||<br />
|-<br />
| [[Elmer E|Elmer Eskil]]||Wallenberg Neuroscience Center, Lund, SE||Ex vivo respiratory capacity of human blood cells - a SUITable application of the O2k Oxygraph / A novel pharmacological approach to counteract metabolic crisis.<br />
|-<br />
| [[Felding-Habermann B|Felding-Habermann Brunhilde]]||The Scripps Research Institute, La Jolla, CA, US||Mitochondrial functions in breast cancer progression.<br />
|-<br />
| [[Goodpaster BH|Goodpaster Bret]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Gnaiger E|Gnaiger Erich]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Hahn D|Hahn Dagmar]]||Inselspital Bern, CH||<br />
|-<br />
| [[Hand SC|Hand Steven C]]||Louisiana State University, LA, US||Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
|-<br />
| [[Harrison DK|Harrison David K]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Hepple_RT| Hepple Russel T.]]||McGill University, Montreal, CA||Mitochondrial involvement in aging muscle: cause and effect(or).<br />
|-<br />
| [[Hey-Mogensen M|Hey-Mogensen Martin]]||Novo Nordisk A/S Diabetes NBEs & Obesity Biology, Måløv, DK||<br />
|-<br />
| [[Jana Prado F|Jana Prado Fabian]]||University of Chile, CL||<br />
|-<br />
| [[Kane DA| Kane Daniel A.]]||St. Francis Xavier University, Antigonish, CA||<br />
|-<br />
| [[Kraunsoe R|Kraunsoe Regitze]]||University of Copenhagen, DK||<br />
|-<br />
| [[Koopman WJ|Koopman Werner J]]||Radboud University Nijmegen Medical Centre, NL||Redox regulation of mitochondrial dynamics.<br />
|-<br />
| [[Kowaltowski AJ|Kowaltowski Alicia J.]]||University of Sao Paulo, BR||<br />
|-<br />
| [[Laner V|Laner Verena]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Larsen S|Larsen Steen]]||University of Copenhagen, DK||Normalization of respiration.<br />
|-<br />
| [[Larsson NG|Göran-Larsson Nils]]||Max Planck Institute for the Biology of Ageing, Cologne, DE||Mouse models with mtDNA mutations.<br />
|-<br />
| [[Ojuka E|Ojuka Edward]]||University of Cape Town, ZA||Fission and fusion, mitochondrial structure and function - is there a tight connection?<br />
|-<br />
| [[Perry CG|Perry Christopher G]]||York University, Ontario, CA||Re-visiting critical experimental parameters to control when measuring respiratory kinetics in permeabilized muscle fibres.<br />
|-<br />
| [[Pesta D|Pesta Dominik]]||Yale University School of Medicine, CT, US||<br />
|- <br />
| [[Picard_M|Picard Martin]]||University of Pennsylvania, PA, US||Quantitative regulation of nuclear gene expression by mitochondrial DNA<br />
heteroplasmy.<br />
|-<br />
| [[Pecina P|Pecina Petr]]||Academy of Sciences of the Czech Republic, Prague, CZ||The use of lymphocytes for diagnostics of mitochondrial disorders.<br />
|-<br />
| [[Purhonen J|Purhonen Janne]]||University Helsinki, FI||<br />
|-<br />
| [[Ristow M|Ristow Michael]]||ETH Zurich, CH||Promoting health and lifespan by increasing mitochondrial ROS formation.<br />
|-<br />
| [[Shirihai O|Shirihai Orian]]||The Shirihai Lab at Boston University, MA, US||<br />
|-<br />
| [[Skulachev VP|Skulachev Vladimir]]||Lomonosov Moscow State University, Moscow, RU||Mitochondria are sources of ROS rather than sinks: Physiological effects of mitochondria-targeted antioxidants.<br />
|-<br />
| [[Starkov AA|Starkov Anatoly]]||Weill Medical College Cornell University, NY, US||<br />
|-<br />
| [[Stiban J|Stiban Johnny]]||Birzeit University, Ramallah, PS||Very long chain ceramides interfere with C16-ceramide-induced channel formation: A plausible mechanism for regulating the initiation of intrinsic apoptosis.<br />
|-<br />
| [[Zorzano A|Zorzano Antonio]]||Universitat de Barcelona, ES||Metabolic implications of mitofusin 2 dysfunction.<br />
|-<br />
| [[Sumbalova Z|Sumbalova Zuzana]]||Comenius University, Bratislava, SK||<br />
|}<br />
<br />
== Venue ==<br />
<br />
The symposium will be hosted in the '''University Center in Obergurgl''' 100 km from Innsbruck. The closest airport is in Innsbruck. Participants arriving at Munich airport will take the train or an airport shuttle to Innsbruck. We provide support in arranging the transfer between Munich and Innsbruck. A bus transfer is organized between Innsbruck and Obergurgl.<br />
<br />
The Obergurgl University Center is located at an altitude of 1940 meters above sea-level. The historical buildings, carefully refurbished and equipped with the latest technical systems, can accommodate around 70 persons in 43 bedrooms and includes conference rooms, the Alpine Research Center, laboratory, sports medicine, lounges and the restaurant. All rooms are equipped with an Internet connection. Additional participants are accommodated in smaller guest houses in 5 minutes walking distance. Meals are served for all participants in the University Center. Two conference rooms for a total of 200 persons and three small rooms for round table discussions are available. There is enough space for poster presentations and instrument exhibitions. The cosy bar and lounge area with an open fire place adds to the familiar atmosphere and supports the interactive spirit of the symposium.<br />
<br />
The small village of Obergurgl is situated in an impressive landscape at about 1900 m above sea level in the Ötz valley. It is surrounded by mountains, offering an exceptional view to the Ötz valley glaciers. During summer time the area is famous for hiking and for skiing in winter time. On the doorstep: one of Europe´s finest Biosphere Parks. The unique surroundings offer the possibility of combining scientific discussions while exploring the alpine environment during the famous “walks and talks”. The informal atmosphere of the workshop encourages also students and young post-docs to participate actively in scientific discussions and to develop future collaborations.<br />
<br />
University Centre Obergurgl, Gaisbergweg 5, 6456 Obergurgl, Tyrol, Austria.<br />
Tel.: +43 (0) 512 507 37201 | Fax: +43 (0) 512 507 37400 | Email: obergurgl@uibk.ac.at <br />
<br />
== International programme committee ==<br />
The international programme committee will provide scientific input by active participation, nominate further invited speakers to ensure balanced point/counterpoint introductions, and select the abstracts as a basis for participation (restricted number of participants), for oral 10+5 min presentations, and for flash presentations.<br />
<br />
Chair:<br />
* [[Gnaiger E|Gnaiger Erich]], Medical University Innsbruck, AT. - erich.gnaiger@mitophysiology.org<br />
Members:<br />
* [[Ali SS|Ali Sameh S]], Helmy Institute of Medical Sciences, Giza, Egypt<br />
* [[Borutaite V|Borutaite Vilma]], Lithuanian University of Health Sciences Kaunas, LT<br />
* [[Neurocon|Chakrabarti Sasanka]], Institute of Post-graduate Medical Education & Research, Kolkatta, India<br />
* [[Chinese Society of Mitochondrial Research and Medicine|Chen Quan]], Chinese Academy of Sciences, Nankai University, Beijing <br />
* [[Dela F|Dela Flemming]], University of Copenhagen, DK <br />
* [[Duchen MR|Duchen Michael R]], University College London, UK<br />
* [[Elmer E|Elmér Eskil]], Lund University, SE <br />
* [[Hand SC|Hand Steven]], Louisiana State University, LA, US<br />
* [[Hickey AJ|Hickey Anthony J]], University of Auckland, NZ<br />
* [[Koopman WJ|Koopman Werner J]], Radboud Univ Nijmegen Medical Centre, NL<br />
* [[Tanaka M|Tanaka Masashi]], Toyko Metropolitan Institute of Gerontology, JP<br />
* [[Neufer PD|Neufer P Darrell]], East Carolina University, Greenville, NC, US<br />
* [[Ojuka E|Ojuka Edward]], University of Cape Town, South Africa<br />
* [[Pecina P|Pecina Petr]], Academy of Sciences of the Czech Republic, CZ<br />
* [[Perry CG|Perry Christopher G]], York University, Toronto, Canada<br />
* [[Rossignol R|Rossignol Rodrigue]], Université Victor Segalen-Bordeaux, FR<br />
* [[Vercesi AE|Vercesi Anibal E]], State University of Campinas, Sao Paulo, Brazil<br />
* [[Taiwan Society for Mitochondrial Research and Medicine|Wei Yau-Huei]], Mackay Medical College, New Taipei City, Taiwan<br />
* [[Zorzano A|Zorzano Antonio]], Universitat de Barcelona, Spain<br />
<br />
== Information for invited speakers==<br />
<br />
MiP2014 will be held at the University Centre Obergurgl, with maximum capacity of 124 persons and a price per person per night of € 65.- including a shared double room, breakfast, and dinner. MiP''conferences'' are organized as small meetings facilitating communication between senior and junior participants. In the tradition of MiP''conferences'', we are asking invited speakers to cooperate and try to receive local support for joining us at the prestigious Mitochondrial Physiology Conference (limited to 124 participants). Support will be provided to students and young investigators.<br />
<br />
== Post-conference O2k-Workshop ==<br />
<br />
A post-conference workshop is organized on the same venue, for hands-on training in mitochondrial respiratory physiology, introducing the combined measurement of high-resolution respirometry and fluorometry (OROBOROS INSTRUMENTS). Since participants come from all-over-the-world, including many students, the combination of the symposium with the workshop makes travelling more economical for the maximum of 24 workshop-participants. The post-conference workshop is financially completely separate from the symposium.<br />
<br />
>> '''[[MitoGlobal_Events_2014]]'''<br />
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<br />
[[Category:MitoPedia]]<br />
[[Category:All]]</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MiPNet04.05_Titration-Injection&diff=58186MiPNet04.05 Titration-Injection2014-03-25T13:59:59Z<p>Bauer Christiane: </p>
<hr />
<div>{{Publication<br />
|title=Gnaiger E, Rieger G (1999) From step titration to ramp injection: Uncoupling by FCCP with TIP. Mitochondr Physiol Network 04.05.<br />
|info=[[Media:MiPNet04.05 Titration-Injection.pdf|'''Open Access-MiPNet04.05.pdf''']]; [http://www.oroboros.at/?tip2k Tip2k]; [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]<br />
|authors=OROBOROS<br />
|year=1999<br />
|journal=Mitochondr Physiol Network<br />
|abstract=Fully supported by the O2k-Core and control of the TIP2k by the software DatLab: The TIP2k can be programmed for multiple titrations and continuous injections. As an alternative to traditional step titration, the TIP offers the new option of ramp injection, providing maximum resolution of the concentration dependence of oxygen flux. This is illustrated by the recording of cellular respiratory flux as a function of a continuous increase of uncoupler (FCCP) concentration.<br />
<br />
''Titration:''<br />
Programmable, automatic titration regimes, with titration volumes of 0.05 to 250 µl, variable titration intervals and duration of titration pulses.<br />
<br />
<br />
''Injection:''<br />
Steady-state injection: Operation at quasi steady-states by continuous injection of substrates at limiting rates of consumption, providing new flexibility in experimental design by combining the technical advantages of closed and open systems. Programmable injection flows: 0.01 to 25 µl.s-1.<br />
Ramp injection (MiPNet04.05, see above): Ramp increase of effector concentrations by "continuous titration".<br />
DatLab software for feedback control by the the TIP2k: for steady-state respirometry at selected oxygen levels and pH-stat applications.<br />
<br />
|mipnetlab=AT_Innsbruck_OROBOROS<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}<br />
{{Labeling<br />
|area=Respiration, Instruments;methods<br />
|organism=Human<br />
|tissues=Endothelial; Epithelial; Mesothelial Cell<br />
|model cell lines=HUVEC<br />
|preparations=Intact cells<br />
|topics=Uncoupler<br />
|instruments=Oxygraph-2k, TIP2k, Protocol<br />
|discipline=Mitochondrial Physiology<br />
|articletype=Protocol; Manual, MiPNet-online Publication<br />
}}</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=O2k-Titration_Set&diff=58109O2k-Titration Set2014-03-20T09:00:48Z<p>Bauer Christiane: </p>
<hr />
<div>{{Mainpage orobopedia}}<br />
{{Product<br />
|description=The '''Titration Set''' consists of 6x 10 µl, 6x 25 µl, and 2x 50 µl Hamilton glass syringes, and 3 spare plungers for the 10 µl syringes. It is a basic HRR-Accessory included in the [[O2k-Core]] Unit.<br />
|product id=20820-01<br />
|product type=O2k, O2k-Module, O2k-Core, Microsyringe, Catalogue<br />
|info=[[MiPNet09.12 O2k-Titrations]]<br />
|product image=[[Image:Hamilton Syringes for Manual Titration.jpg|right|180px|link=http://www.bioblast.at/index.php/Titration_Set]]<br />
}}<br />
<br />
<br />
<br />
== The '''Titration Set''' ''consists of'' ==<br />
<br />
:6 [[Microsyringe\10 mm3 51/0.13 mm]] 10 mm<sup>3</sup><br />
:6 [[Microsyringe\25 mm3 51/0.15 mm]] 25 mm<sup>3</sup> <br />
:2 [[Microsyringe\50 mm3 51/0.15 mm]] 50 mm<sup>3</sup><br />
:3 [[Plunger\10 mm3]] for 10 mm<sup>3</sup> syringe<br />
<br />
<br />
== '''Handling of the syringes''' ==<br />
===General information===<br />
Use separate syringes for substrates, uncoupler and inhibitors.<br />
<br />
Colour code of syringes:<br />
* Substrate: white<br />
* Uncoupler: blue<br />
* Inhibitors: red<br />
* Digitonin: yellow<br />
<br />
===Preparation of syringes before experiment===<br />
* Prepare syringes according to [[SUIT protocol]] and [[MiPNet09.12 O2k-Titrations | O2k-Titrations]].<br />
* Prepare a rack with three 50 ml falcons for washing procedure:<br />
:* First rinse (distilled water) <br />
:* Distilled water<br />
:* 100% EtOH <br />
:* Prepare beaker for waste<br />
* Washing procedure:<br />
:* Discard residual chemical into the beaker<br />
:* Plunge syringe in 'first rinse' falcon (distilled water) and wipe it with a small kimwipe. Take care to rinse not only the needle itself but also the needle base<br />
:* Wash syringe three times in falcon filled with distilled water or 100% EtOH (depends on whether reagents were dissolved in distilled water or EtOH).<br />
:* Syringes used for AmR or CCCP: wash twice with DMSO (flush syringe surface with distilled water between washing steps).<br />
<br />
===Syringes in use===<br />
* Hold the syringe on the top to prevent warming of the glass.<br />
* Fill the syringe above desired mark in order to have a little bit of excess. <br />
* Take care not to produce bubbles when filling the syringe – this causes inaccuracies in measurement.<br />
* Fill the syringe not too fast but steady. Pumping is also ok.<br />
* Before titrating the chemical into the chamber: carefully press plunger and check if a small drop appears on top of the needle <br />
* Be swift when titrating in the chamber, especially when titrating EtOH due to its volatile nature. <br />
* After titrating in the chamber, discard the residual chemical into the beaker and rinse the outside of the syringe with distilled water (plunging in the falcon labelled 'first rinse') and dab it gently using small kimwipes. <br />
* Store syringes in 15 ml glass epprouvettes during the experiment in used solvent (distilled water or EtOH, use a separate epprouvette for every syringe). <br />
* Caution: take care to specifically rinse the syringe used for ADP (with MgCl2) IMMEDIATELY after use (on the surface but more importantly inside 3 times with distilled water) as a precipitate may form and cause blocking of the needle.<br />
<br />
===Preparation of syringes after experiment and storage===<br />
* Take care of the washing order: first substrate-, then uncoupler- and finally inhibitor syringes.<br />
* Wash three times with last used solvent, take care again to rinse also the needle base, rinse three times with EtOH 100% (use a small beaker).<br />
* Store syringes in dry condition. <br />
<br />
<br />
<u>Note:</u><br> <br />
When using a syringe with a chemical different from the usually assigned one then a washing step with distilled water is crucial to prevent medium contamination by carry-over!<br />
<br />
===Further useful information===<br />
* Visit also: [http://www.hamiltoncompany.com/Syringes/ Hamilton] and [http://www.hamiltoncompany.com/Syringes/techInfo/PDFs/L20058_care_use_guide.pdf Hamilton info pdf]<br />
* Eliminate rugh edges with 3M™ Wetordry™ Paper Sheet P1000 by gentle rubbing<br />
* Use a cleaning wire for the needle. If a syringe is clocked , sometimes it is also helpful to fill it from the back with EtOH or H2O and try to press the plunger gentle down.</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=Gnaiger_2020_BEC_MitoPathways&diff=58098Gnaiger 2020 BEC MitoPathways2014-03-19T15:05:18Z<p>Bauer Christiane: </p>
<hr />
<div>{{Publication<br />
|title=Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp. ISBN 978-3-9502399-6-6<br />
|info=[[Media:Gnaiger 2012 Mitochondr Physiol Network MitoPathways.pdf| '''Bioblast pdf''']]; [http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger_2012_MitoPathways]<br />
|authors=Gnaiger E<br />
|year=2012<br />
|journal=Mitochondr Physiol Network<br />
|keywords=Q-junction, Respiratory states, Flux control ratios<br />
|mipnetlab=AT_Innsbruck_Gnaiger E, AT Innsbruck OROBOROS, AT Innsbruck MitoCom<br />
|discipline=Mitochondrial Physiology<br />
}}<br />
{{Labeling<br />
|area=Respiration, Instruments;methods, mt-Biogenesis;mt-density, Comparative MiP;environmental MiP, Exercise physiology;nutrition;life style, mt-Medicine, mt-Awareness<br />
|organism=Human, Mouse<br />
|tissues=Heart, Skeletal muscle<br />
|model cell lines=Fibroblast<br />
|preparations=Permeabilized cells, Permeabilized tissue, Isolated Mitochondria<br />
|topics=Coupling efficiency;uncoupling, Flux control, Threshold;excess capacity<br />
|couplingstates=LEAK, ROUTINE, OXPHOS, ETS<br />
|substratestates=CI, CII, ETF, GpDH, CI+II<br />
|instruments=Oxygraph-2k, Protocol, Theory<br />
|discipline=Mitochondrial Physiology<br />
}}<br />
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[[File:BlueBook openAccess.png|right|280px]]<br />
<br />
__TOC__<br />
<br />
<br />
= Bioblast online references and notes =<br />
* '''Preface''' <br />
: '''References Preface''' <br />
# [[Gnaiger 2007 MitoPathways|Gnaiger E ed (2007) Mitochondrial Pathways and Respiratory Control. 1st ed. OROBOROS MiPNet Publications, Innsbruck: 96 pp]]<br />
<br />
:>> [[MiPNet_Reference_Laboratories|WorldWide MiPNet]]<br />
:>> [http://www.oroboros.at/?O2k-Protocols O2k-Protocols]<br />
:>> [[O2k-Publications]]<br />
:>> [[MitoPedia Glossary: Terms and abbreviations]]<br />
<br />
<br />
== Chapter 1. OXPHOS analysis ==<br />
=== References OXPHOS analysis ===<br />
# [[Altmann 1894 Verlag Von Veit & Comp | Altmann R (1894) Die Elementarorganismen und ihre Beziehungen zu den Zellen. Zweite vermehrte Auflage. Verlag Von Veit & Comp, Leipzig. 160 pp, 34 Tafeln.]]<br />
# [[Dawson 2005 J Physiol | Dawson KD, Baker DJ, Greenhaff PL, Gibala MJ (2005) An accute decrease in TCA cycle intermediates does not affect aerobic energy delivery in contracting rat skeletal muscle. J Physiol 565: 637-643.]]<br />
# [[Garlid 1993 BTK | Garlid KD, Semrad C, Zinchenko V (1993) Does redox slip contribute significantly to mitochondrial respiration? In: Schuster S, Rigoulet M, Ouhabi R, Mazat J-P (eds) Modern Trends in Biothermokinetics. Plenum Press, New York, London: 287-293.]]<br />
# [[Gibala 1998 Am J Physiol Endocrinol Metab | Gibala 1998 Am J Physiol Endocrinol Metab|Gibala MJ, MacLean DA, Graham TE, Saltin B (1998) Tricarboxylic acid cycle intermediate pool size and estimated cycle flux in human muscle during exercise. Am J Physiol Endocrinol Metab 275: E235-E242.]] - concentrations of TCA cycle intermediates.<br />
# [[Gnaiger 1983 J Exp Zool|Gnaiger E (1983) Heat dissipation and energetic efficiency in animal anoxibiosis. Economy contra power. J Exp Zool 228: 471-490.]]<br />
# [[Gnaiger 1993 Hypoxia|Gnaiger E (1993) Efficiency and power strategies under hypoxia. Is low efficiency at high glycolytic ATP production a paradox? In: Surviving Hypoxia: Mechanisms of Control and Adaptation. Hochachka PW, Lutz PL, Sick T, Rosenthal M, Van den Thillart G (eds) CRC Press, Boca Raton, Ann Arbor, London, Tokyo: 77-109.]] - The Gibbs force of phorphorylation of ADP to ATP is ''F''<sub>ATP</sub> = 52 to 66 kJ/mol ATP under intracellular conditions.<br />
# [[Gnaiger 1993 Pure Appl Chem|Gnaiger E (1993) Nonequilibrium thermodynamics of energy transformations. Pure Appl Chem 65: 1983-2002.]]<br />
# [[Gnaiger 2003 Adv Exp Med Biol|Gnaiger E (2003) Oxygen conformance of cellular respiration. A perspective of mitochondrial physiology. Adv Exp Med Biol 543: 39-55.]]<br />
# [[Gnaiger 2002 Biochem Soc Trans|Gnaiger E, Kuznetsov AV (2002) Mitochondrial respiration at low levels of oxygen and cytochrome c. Biochem Soc Trans 30: 252-258.]]<br />
# [[Gnaiger 2000 Life in the Cold|Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000b) Mitochondria in the cold. In: Life in the Cold. (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York: pp 431-442.]] – MiR05 as the basis of MiR06.<br />
# [[Gnaiger 1998 Biochim Biophys Acta | Gnaiger E, Lassnig B, Kuznetsov AV, Margreiter R (1998) Mitochondrial respiration in the low oxygen environment of the cell: Effect of ADP on oxygen kinetics. Biochim Biophys Acta 1365: 249-254.]]<br />
# [[Gnaiger 1998 J Exp Biol | Gnaiger E, Lassnig B, Kuznetsov AV, Rieger G, Margreiter R (1998) Mitochondrial oxygen affinity, respiratory flux control, and excess capacity of cytochrome c oxidase. J Exp Biol 201: 1129-1139.]]<br />
# [[Gueguen 2005 J Physiol | Gueguen N, Lefaucheur L, Ecolan P, Fillaut M, Herpin P (2005) Ca2+-activated myosin-ATPases, creatine and adenylate kinases regulate mitochondrial function according to myofibre type in rabbit. J Physiol 564: 723-735.]]<br />
# [[Hatefi 1962 J Biol Chem | Hatefi Y, Haavik AG, Fowler LR, Griffiths DE (1962) Studies on the electron transfer system. XLII. Reconstitution of the electron transfer system. J Biol Chem 237: 2661-2669.]]<br />
# [[Kuznetsov 2004 Am J Physiol Heart Circ Physiol | Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome c release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286: H1633–H1641.]] – Cytochrome ‘’c’’ test.<br />
# [[Lemieux 2006 Biochim Biophys Acta | Lemieux H, Garedew A, Blier PU, Tardif J-C, Gnaiger E (2006) Temperature effects on the control and capacity of mitochondrial respiration in permeabilized fibers of the mouse heart. Biochim Biophys Acta, EBEC Short Reports Suppl 14: 201-202.]]<br />
# [[Mitchell 1961 Nature | Mitchell P (1961) Coupling of phosphorylation to electron and hydrogen transfer by a chemi-osmotic type of mechanism. Nature 191: 144-148.]]<br />
# Mitchell P (1966) Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Glynn Research Ltd, Bodmin: 192 pp. - ''The Grey Book'' 1.<br />
# Mitchell P (1968) Chemiosmotic coupling and energy transduction. Glynn Research Ltd, Bodmin: 111 pp. - ''The Grey Book'' 2.<br />
# [[Mitchell 1967 Biochem J | Mitchell P, Moyle J (1967) Respiration-driven proton translocation in rat liver mitochondria. Biochem J 105: 1147-1162.]]<br />
# [[Mootha 1997 Am J Physiol | Mootha VK, Arai AE, Balaban RS (1997) Maximum oxidative phosphorylation capacity of the mammalian heart. Am J Physiol 272: H769-H775.]] – [Pi] <10 mM and [ADP] <0.4 mM limit OXPHOS in isolated heart mitochondria.<br />
# [[Nicholson 2012 Nature | Nicholson JK, Holmes E, Kinross JM, Darzi AW, Takats Z, Lindon JC (2012) Metabolic phenotyping in clinical and surgical environments. Nature 491: 384-392.]]<br />
# [[Owen 2002 J Biol Chem | Owen OE, Kalhan SC, Hanson RW (2002) The key role of anaplerosis and cataplerosis for citric acid cycle function. J Biol Chem 277: 30409-30412.]]<br />
# [[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]] - >90% saturation is reached only >5 mM ADP, yet few studies use such high [ADP] in permeabilized tissues and cells. - Oxygen limitation of respiration below air saturation.<br />
# [[Puchowicz 2004 Mitochondrion | Puchowicz MA, Varnes ME, Cohen BH, Friedman NR, Kerr DS, Hoppel CL (2004) Oxidative phosphorylation analysis: assessing the integrated functional activity of human skeletal muscle mitochondria – case studies. Mitochondrion 4: 377-385.]] - Cytochrome ''c'' test.<br />
# [[Rasmussen 2000 Mol Cell Biochem | Rasmussen UF, Rasmussen HN (2000) Human quadriceps muscle mitochondria: A functional characterization. Mol Cell Biochem 208: 37-44.]] - Cytochrome ''c'' test.<br />
# [[Renner 2003 Biochim Biophys Acta | Renner K , Amberger A, Konwalinka G, Gnaiger E (2003) Changes of mitochondrial respiration, mitochondrial content and cell size after induction of apoptosis in leukemia cells. Biochim Biophys Acta 1642: 115-123.]]<br />
# [[Rossignol 2003 Biochem J | Rossignol R, Faustin B, Rocher C, Malgat M, Mazat JP, Letellier T (2003) Mitochondrial threshold effects. Biochem J 370: 751-762.]]<br />
# [[Saks 1998 Mol Cell Biochem | Saks VA, Veksler VI, Kuznetsov AV, Kay L, Sikk P, Tiivel T, Tranqui L, Olivares J, Winkler K, Wiedemann F, Kunz WS (1998) Permeabilised cell and skinned fiber techniques in studies of mitochondrial function in vivo. Mol Cell Biochem 184: 81-100.]] - The apparent ''K''<sub>m</sub> for ADP increases up to 0.5 mM in some permeabilized muscle fibres.<br />
# [[Territo 2000 Am J Physiol Cell Physiol| Territo PR, Mootha VK, French SA, Balaban RS (2000) Ca<sup>2+</sup> activation of heart mitochondrial oxidative phosphorylation: role of the F0/F1-ATPase. Am J Physiol Cell Physiol 278: C423-C435.]]<br />
<br />
== Chapter 2. Mitochondrial pathways to Complex I: Respiratory substrate control with pyruvate, malate and glutamate ==<br />
<br />
=== References CI ===<br />
# Brandt U (2006) Energy converting NADH:quinone oxidoreductase (Complex I). Annu Rev Biochem 75: 69-92.<br />
# Brewer GJ, Jones TT, Wallimann T, Schlattner U (2004) Higher respiratory rates and improved creatine stimulation in brain mitochondria isolated with antioxidants. Mitochondrion 4: 49-57.<br />
# Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. III. The steady state. J Biol Chem 217: 409-427. - Substrate depletion in isolated mitochondria is achieved in State 2: ADP is added to induce a transient stimulation of oxygen flux based on oxidation of endogenous substrates. <br />
# Digerness SB, Reddy WJ (1976) The malate-aspartate shuttle in heart mitochondria. J Mol Cell Cardiol. 8: 779-785.<br />
# Duchen MR (2004) Roles of mitochondria in health and disease. Diabetes 53, Suppl 1: S96-S102. - Mitochondrial glutamate dehydrogenase is particularly active in astrocytes, preventing glutamate induced neurotoxicity.<br />
# [[Gnaiger 2009 Int J Biochem Cell Biol|Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837–1845.]]<br />
# Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97: 11080-11085. - Equilibrium ratio of malate to fumarate is 4.1.<br />
# Hildyard JCW, Halestrap AP (2003) Identification of the mitochondrial pyruvate carrier in ''Saccharomyces cerevidiae''. Biochem J 374: 607-611.<br />
# Johnson G, Roussel D, Dumas JF, Douay O, Malthiery Y, Simard G, Ritz P (2006) Influence of intensity of food restriction on skeletal muscle mitochondrial energy metabolism in rats. Am J Physiol Endocrinol Metab 291: E460-E467. - Uncoupling stimulates coupled OXPHOS respiration, PM<sub>''P''</sub>, by 14%.<br />
# Kemp RB, Hoare S, Schmalfeldt M, Bridge CM, Evans PM, Gnaiger E (1994) A thermochemical study of the production of lactate by glutaminolysis and glycolysis in mouse macrophage hybridoma cells. In What is Controlling Life? (Gnaiger E, Gellerich FN, Wyss M, eds) Modern Trends in BioThermoKinetics 3, Innsbruck Univ Press: 226-231. - Glutamate derived from hydrolyzation of glutamine is a very important aerobic substrate in cultured cells.<br />
# Lemasters JJ (1984) The ATP-to-oxygen stoichiometries of oxidative phosphorylation by rat liver mitochondria. J Biol Chem 259: 13123-13130. - Malonate added to inhibit the succinate-fumarate reaction exerts only a minor effect on liver mitochondrial respiration.<br />
# Maechler P, Carobbio S, Rubi B (2006) In beta-cells, mitochondria integrate and generate metabolic signals controlling insulin secretion. Int J Biochem Cell Biol 38: 696-709.<br />
# Messer JI, Jackman MR, Willis WT (2004) Pyruvate and citric acid cycle carbon requirements in isolated skeletal muscle mitochondria. Am J Physiol Cell Physiol 286: C565-572. - With malate alone and saturating [ADP] isolated rat skeletal muscle mitochondria respire at only 1.3% of OXPHOS capacity with pyruvate+malate. Pyruvate alone yields only 2.1% of OXPHOS capacity (State P) with PM.<br />
# Nicholls DG, Ferguson SJ (2002) Bioenergetics 3, Academic Press, London. 287 pp. - Carriers.<br />
# Ouhabi R, Boue-Grabot M, Mazat J-P (1994) ATP synthesis in permeabilized cells: Assessment of the ATP/O ratios in situ. In What is Controlling Life? (Gnaiger E, Gellerich FN, Wyss M, eds) Modern Trends in BioThermoKinetics 3, Innsbruck Univ Press: 141-144. - In fibroblasts, GM<sub>''P''</sub> supports a higher respiratory flux than PM<sub>''P''</sub>.<br />
# O’Donnell JM, Kudej RK, LaNoue KF, Vatner SF, Lewandowski ED (2004) Limited transfer of cytosolic NADH into mitochondria at high cardiac workload. Am J Physiol Heart Circ Physiol 286: H2237-H2242.<br />
# Puchowicz MA, Varnes ME, Cohen BH, Friedman NR, Kerr DS, Hoppel CL (2004) Oxidative phosphorylation analysis: assessing the integrated functional activity of human skeletal muscle mitochondria – case studies. Mitochondrion 4: 377-385. - OXPHOS with glutamate alone is 50% to 85% of respiration with glutamate+malate. Accumulation of fumarate inhibits succinate dehydrogenase and glutamate dehydrogenase (Caughey et al 1957; Dervartanian, Veeger 1964). - OXPHOS with glutamate+malate is identical or 10% higher than with pyruvate+malate.<br />
# Rasmussen UF, Rasmussen HN (2000) Human quadriceps muscle mitochondria: A functional characterization. Mol Cell Biochem 208: 37-44. - Uncoupling stimulates coupled OXPHOS respiration, PMP, by 15% in human skeletal muscle. OXPHOS with glutamate alone is 50% to 85% of respiration with glutamate+malate. - OXPHOS with glutamate+malate is identical or 10% higher than with pyruvate+malate.<br />
# Thomas et al (2004) - OXPHOS in human skeletal muscle for PM<sub>''P''</sub> is 25% higher than for GM<sub>''P''</sub>.<br />
# Winkler-Stuck K, Kirches E, Mawrin C, Dietzmann K, Lins H, Wallesch CW, Kunz WS, Wiedemann FR (2005) Re-evaluation of the dysfunction of mitochondrial respiratory chain in skeletal muscle of patients with Parkinson's disease. J Neural Transm 112: 499-518. - OXPHOS in human skeletal muscle for PM<sub>''P''</sub> is 16% higher than for GM<sub>''P''</sub>.<br />
<br />
=== Notes CI ===<br />
# Schwerzmann et al (1989) Proc Natl Acad Sci U S A 86: 1583-1587. “Of the substrates used here, pyruvate/malate activates the chain at complex I, glutamate/malate and succinate at complexes II and III, ..” - This consideration of glutamate+malate requires correction.<br />
# Ponsot et al (2005) J Cell Physiol 203: 479-486. (a) Respiration (State 3) in permeabilized fibres with malate alone gave 25-50% of the flux with pyruvate+malate. This most likely indicates a large content of endogenous mitochondrial substrates, which interfere to an unknown degree with the kinetics of respiration after addition of exogenous substrates. In such a study, the conventional initial depletion of endogenous substrates would be most important. (b) Maximal respiration rates in muscle should be evaluated at saturating or high Pi, since at a Pi concentration of 3 mM OXPHOS respiration is phosphate limited.<br />
# Hulbert et al (2006) J Comp Physiol B 176: 93-105. Addition of ‘sparking malate concentrations’. This term can probably be derived from the misconception that tricarboxylic acid cycle intermediates are conserved during respiration of isolated mitochondria. 380 µM malate (instead of mM concentrations) in conjunction with 2.4 mM pyruvate were used, which makes a comparison difficult between different tissues and different species: the low malate concentration may limit PMP flux at various degrees in the different sources of mitochondria, and GMP may support higher fluxes than PMP at tissue- and species-specific degrees.<br />
<br />
<br />
== Chapter 3. Mitochondrial pathways to Complex II. Glycerophosphate dehydrogenase and electrontransferring flavoprotein ==<br />
<br />
=== References CII ===<br />
# Capel F, Rimbert V, Lioger D, Diot A, Rousset P, Patureau Mirand P, Boirie Y, Morio B, Mosoni L (2005) Due to reverse electron transfer, mitochondrial H2O2 release increases with age in human vastus lateralis muscle although oxidative capacity is preserved. Mech Ageing Develop 126: 505-511. - With succinate alone OXPHOS is 30-40% lower than with succinate+rotenone in human skeletal muscle mitochondria.<br />
# Cecchini G (2003) Function and structure of Complex II of the respiratory chain. Annu Rev Biochem 72: 77-109.<br />
# Ernster L, Nordenbrand K (1967) Skeletal muscle mitochondria. In: Estabrook RW, Pullman ME (eds) Meth Enzymol: 86-94. – With succinate alone OXPHOS is 30-40% lower than with succinate+rotenone in rat skeletal muscle mitochondria.<br />
# Jackman MR, Willis WT (1996) Characteristics of mitochondria isolated from type I and type IIb skeletal muscle. Am J Physiol Cell Physiol 270: C673-678. - Glycerophosphate oxidation is 10-fold higher in rabbit gracilis mitochondria compared to soleus.<br />
# Lehninger AL (1970) Biochemistry. The molecular basis of cell structure and function Worth. 833 pp. - Oxaloacetate is a more potent competitive inhibitor of succinate dehydrogenase than malonate even at small concentration (p. 352).<br />
# Muller FL, Liu Y, Abdul-Ghani MA, Lustgarten MS, Bhattacharya A, Jang YC, Van Remmen H (2008) High rates of superoxide production in skeletal-muscle mitochondria respiring on both Complex I- and Complex II-linked substrates. Biochem J 409: 491–499. - Addition of malate inhibits superoxide production with succinate, probably due to the oxaloacetate inhibition of CII.<br />
# Rauchova H, Drahota Z, Rauch P, Fato R, Lenaz G (2003) Coenzyme Q releases the inhibitory effect of free fatty acids on mitochondrial glycerophosphate dehydrogenase. Acta Biochim Polonica 50: 405-413. - Glycerophosphate is an important substrate for respiration in brown adipose tissue mitochondria.<br />
# Rasmussen UF, Rasmussen HN (2000) Human quadriceps muscle mitochondria: A functional characterization. Mol Cell Biochem 208: 37-44. – Glycerophosphate oxidation is relatively slow.<br />
# Sun F, Huo X, Zhai Y, Wang A, Xu J, Su D, Bartlam M, Rao Z (2005) Crystal structure of mitochondrial respiratory membrane protein Complex II. Cell 121: 1043–1057.<br />
<br />
=== Notes CII === <br />
# Ponsot et al (2005) J Cell Physiol 203: 479-486. ‘.. the mitochondrial form of GPDH, which produces FADH2 within the mitochondrial matrix and provides electrons to Compoex II of the phosphorylation chain’. – The mitochondrial glycerophosphate dehydrogenase (GpDH), located on the outer side of the inner mitochondrial membrane, does not provide electrons to CII, but feeds electrons into the Q-cycle entirely independent of CII. FADH2 is not produced within the mitochondrial matrix. Electron transfer takes place from the mitochondrial inner membrane flavoprotein-linked glycerophosphate dehydrogenase to CoQ.<br />
# In the first edition of ‘Mitochondrial Pathways and Respiratory Control’ (2007), the term ‘electron transport’ is used synonymously for ‘electron transfer’.<br />
<br />
<br />
<br />
== Chapter 4. Mitochondrial pathways to Complexes I+II: Convergent electron transfer at the Q-Junction and additive effect of substrate combinations ==<br />
<br />
=== References CI+II ===<br />
# Aragonés J, Schneider M, Van Geyte K, Fraisl P, Dresselaers T, Mazzone M, Dirkx R, Zacchigna S, Lemieux H, Jeoung NH, Lambrechts D, Bishop T, Lafuste P, Diez-Juan A, K Harten S, Van Noten P, De Bock K, Willam C, Tjwa M, Grosfeld A, Navet R, Moons L, Vandendriessche T, Deroose C, Wijeyekoon B, Nuyts J, Jordan B, Silasi-Mansat R, Lupu F, Dewerchin M, Pugh C, Salmon P, Mortelmans L, Gallez B, Gorus F, Buyse J, Sluse F, Harris RA, Gnaiger E, Hespel P, Van Hecke P, Schuit F, Van Veldhoven P, Ratcliffe P, Baes M, Maxwell P, Carmeliet P (2008) Deficiency or inhibition of oxygen sensor Phd1 induces hypoxia tolerance by reprogramming basal metabolism. Nat Genet 40: 170-180. - OXPHOS analysis for phenotyping.<br />
# Bianchi C, Genova ML, Parenti Castelli G, Lenaz G (2004) The mitochondrial respiratory chain is partially organized in a supercomplex assembly: kinetic evidence using flux control analysis. J Biol Chem 279: 36562-36569.<br />
# Boushel R, Gnaiger E, Schjerling P, Skovbro M, Kraunsoe R, Flemming D (2007) Patients with Type 2 Diabetes have normal mitochondrial function in skeletal muscle. Diabetologia 50: 790-796.<br />
# Boushel R, Gnaiger E, Calbet JA, Gonzalez-Alonso J, Wright-Paradis C, Sondergaard H, Ara I, Helge JW, Saltin B (2011) Muscle mitochondrial capacity exceeds maximal oxygen delivery in humans. Mitochondrion 11: 303-307.<br />
# Capel F, Rimbert V, Lioger D, Diot A, Rousset P, Patureau Mirand P, Boirie Y, Morio B, Mosoni L (2005) Due to reverse electron transfer, mitochondrial H2O2 release increases with age in human vastus lateralis muscle although oxidative capacity is preserved. Mech Ageing Develop 126: 505-511. - CI+II substrate combination.<br />
# Chance B (1965) Reaction of oxygen with the respiratory chain in cells and tissues. J Gen Physiol 49: 163-188. - Glutamate+succinate as respiratory substrate combination, without comparison of flux with different substrates.<br />
# Costa LE, Boveris A, Koch OR, Taquini AC (1988) Liver and heart mitochondria in rats submitted to chronic hypobaric hypoxia. Am J Physiol Cell Physiol 255: C123-C129.<br />
# Digerness SB, Reddy WJ (1976) The malate-aspartate shuttle in heart mitochondria. J Mol Cell Cardiol. 8: 779-785.<br />
# Eberhart K, Rainer J, Bindreither D, Ritter I, Gnaiger E, Kofler R, Oefner PJ, Renner K (2011) Glucocorticoid-induced alterations in mitochondrial membrane properties and respiration in childhood acute lymphoblastic leukemia. Biochim Biophys Acta 1807: 719-725.<br />
# Estabrook R (1967) Mitochondrial respiratory control and the polarographic measurement of ADP:O ratios. Meth Enzymol 10: 41-47.<br />
# Garait B, Couturier K, Servais S, Letexier D, Perrin D, Batandier C, Rouanet J-L, Sibille B, Rey B, Leverve X, Favier R (2005) Fat intake reverses the beneficial effects of low caloric intake on skeletal muscle mitochondrial H2O2 production. Free Radic Biol Med 39: 1249–1261. - GM<sub>''P''</sub>/GMS<sub>''P''</sub> substrate control ratio in skeletal muscle of rats fed on various diets ranges from 0.7 to 0.8. <br />
# Garedew A, Lemieux H, Schachner T, Blier PU, Tardif J-C, Gnaiger E (2006) High excess capacity of cytochrome c oxidase in permeabilized fibers of the mouse heart. Biochim Biophys Acta, EBEC Short Reports Suppl 14: 167-168.<br />
# [[Gnaiger 2009 Int J Biochem Cell Biol|Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837–1845.]]<br />
# Gnaiger E, Wright-Paradis C, Sondergaard H, Lundby C, Calbet JA, Saltin B, Helge J, Boushel R (2005) High-resolution respirometry in small biopsies of human muscle: correlations with body mass index and age. Mitochondr Physiol Network 10.9: 14-15. http://www.mitophysiology.org/?gnaigere.<br />
# González-Flecha B, Cutrin JC, Boveris A (1993) Time course and mechanism of oxidative stress and tissue damage in rat liver subjected to in vivo ischemia-reperfusion. J Clin Invest 91: 456-464. - Respiration was measured in states GS<sub>''P''</sub> and GM<sub>''P''</sub>.<br />
# Gutman M, Coles CJ, Singer TP, Casida JE (1971) On the functional organization of the respiratory chain at the dehydrogenase-coenzyme Q junction. Biochemistry 10: 2036-2043.<br />
# Hansford RG, Hogue BA, Mildaziene V (1997) Dependence of H2O2 formation by rat heart mitochondria on substrate availability and donor age. J Bioenerg Biomembr 29: 89–95.<br />
# Hatefi Y, Haavik AG, Fowler LR, Griffiths DE (1962) Studies on the electron transfer system. XLII. Reconstitution of the electron transfer system. J Biol Chem 237: 2661-2669.<br />
# König T, Nicholls DG, Garland PB (1969) The inhibition of pyruvate and Ls(+)-isocitrate oxidation by succinate oxidation in rat liver mitochondria. Biochem J 114: 589-596.<br />
# Krebs HA (1935) CXCVII. Metabolism of amino-acids. III. Deamination of amino-acids. Biochem J 29: 1620-1644.<br />
# Kunz WS, Kudin A, Vielhaber S, Elger CE, Attardi G, Villani G (2000) Flux control of cytochrome c oxidase in human skeletal muscle. J Biol Chem 275: 27741-27745.<br />
# Kuznetsov AV, Clark JF, Winkler K, Kunz WS (1996) Increase of flux control of cytochrome c oxidase in copper-deficient mottled brindled mice. J Biol Chem 271: 283-288.<br />
# Kuznetsov AV, Strobl D, Ruttmann E, Königsrainer A, Margreiter R, Gnaiger E (2002) Evaluation of mitochondrial respiratory function in small biopsies of liver. Analyt Biochem 305: 186-194. - S(Rot) alone supports a higher flux than GM in liver mitocondria.<br />
# Kuznetsov AV, Winkler K, Kirches E, Lins H, Feistner H, Kunz WS (1997) Application of inhibitor titrations for the detection of oxidative phosphorylation defects in saponin-skinned muscle fibers of patients with mitochondrial diseases. Biochim Biophys Acta 1360: 142-150. - OXPHOS with glutamate+malate is identical or 10% higher than with pyruvate+malate.<br />
# LaNoue KF, Bryla J, Williamson JR (1972) Feedback interactions in the control of citric acid cycle activity in rat heart mitochondria. J Biol Chem 247: 667-679.<br />
# LaNoue KF, Schoolwerth AC (1979) Metabolite transport in mitochondria. Annu Rev Biochem 48: 871-922.<br />
# Lehninger AL (1970) Biochemistry. The molecular basis of cell structure and function Worth. 833 pp. - Electron transport chain.<br />
# Lemieux H, Garedew A, Blier PU, Tardif J-C, Gnaiger E (2006) Temperature effects on the control and capacity of mitochondrial respiration in permeabilized fibers of the mouse heart. Biochim Biophys Acta, EBEC Short Reports Suppl 14: 201-202.<br />
# [[Lemieux 2011 Int J Biochem Cell Biol|Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43: 1729–1738.]] - SUIT protocols.<br />
# Lenaz G, Genova ML (2009) Structural and functional organization of the mitochondrial respiratory chain: A dynamic super-assembly. Int J Biochem Cell Biol 41: 1750-1772.<br />
# Llesuy S, Evelson P, González-Flecha B, Peralta J, Carreras MC, Poderoso JJ, Boveris A (1994) Oxidative stress in muscle and liver of rats with septic syndrome. Free Radic Biol Med 16: 445-451. - GM<sub>''P''</sub>/GS<sub>''P''</sub> substrate control ratios are 0.8 and 0.7 in liver mitochondria (male Wistar and female Sprague-Dawley, respectively).<br />
# Mogensen M, Sahlin K (2005) Mitochondrial efficiency in rat skeletal muscle: influence of respiration rate, substrate and muscle type. Acta Physiol Scand 185: 229-236. - The flux control ratio of palmitoylcarnitine+malate/PM (OXPHOS) is 0.6 for mitochondria isolated from rat extensor digitorum longus muscle (mainly type II fibre type), but is 0.95 in rat soleus muscle (type I fibre type).<br />
# Muller FL, Liu Y, Abdul-Ghani MA, Lustgarten MS, Bhattacharya A, Jang YC, Van Remmen H (2008) High rates of superoxide production in skeletal-muscle mitochondria respiring on both Complex I- and Complex II-linked substrates. Biochem J 409: 491–499.<br />
# Nicholls DG, Ferguson SJ (2002) Bioenergetics 3, Academic Press, London. 287 pp. - Electron transport chain.<br />
# [[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]] - SUIT protocols.<br />
# [[Pesta 2011 Am J Physiol Regul Integr Comp Physiol|Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.]] - SUIT protocols.<br />
# Rasmussen HN, Rasmussen UF (1997) Small scale preparation of skeletal muscle mitochondria, criteria for integrity, and assays with reference to tissue function. Mol Cell Biochem 174: 55-60.<br />
# Rasmussen UF, Rasmussen HN (2000) Human quadriceps muscle mitochondria: A functional characterization. Mol Cell Biochem 208: 37-44. - OXPHOS with malate+palmitoylcarnitine is 0.60 of OXPHOS with GM.<br />
# Rasmussen UF, Rasmussen HN, Krustrup P, Quistorff B, Saltin B, Bangsbo J (2001) Aerobic metabolism of human quadriceps muscle: in vivo data parallel measurements on isolated mitochondria. Am J Physiol Endocrinol Metab 280: E301–E307.<br />
# Rossignol R, Faustin B, Rocher C, Malgat M, Mazat JP, Letellier T (2003) Mitochondrial threshold effects. Biochem J 370: 751-762.<br />
# Rumsey WL, Schlosser C, Nuutinen EM, Robiolio M, Wilson DF (1990) Cellular energetics and the oxygen dependence of respiration in cardiac myocytes isolated from adult rat. J Biol Chem 265. 15392-15399. - 2.5-fold increase of respiration in the LEAK state with GMS compared to GM.<br />
# Schägger H, Pfeiffer K (2001) The ratio of oxidative phosphorylation complexes I-V in bovine heart mitochondria and the composition of respiratory chain supercomplexes. J Biol Chem 276: 37861-37867.<br />
# Short KR, Bigelow ML, Kahl J, Singh R, Coenen-Schimke J, Raghavakaimal S, Nair KS (2005) Decline in skeletal muscle mitochondrial function with aging in humans. Proc Natl Acad Sci U S A 102: 5618 - 5623.<br />
# Simon J, van Spanning RJM, Richardson DJ (2008) The organisation of proton motive and non-proton motive redox loops in prokaryotic respiratory systems. Biochim Biophys Acta 1777: 1480–1490.<br />
# Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271: C2053-C2061.<br />
# Steinlechner-Maran R, Eberl T, Kunc M, Schröcksnadel H, Margreiter R, Gnaiger E (1997) Respiratory defect as an early event in preservation/reoxygenation injury in endothelial cells. Transplantation 63: 136-142.<br />
# Stadlmann S, Rieger G, Amberger A, Kuznetsov AV, Margreiter R, Gnaiger E (2002) H2O2-mediated oxidative stress versus cold ischemia-reperfusion: mitochondrial respiratory defects in cultured human endothelial cells. Transplantation 74: 1800-1803.<br />
# Sugano T, Oshino N, Chance B (1974). Mitochondrial functions under hypoxic conditions. The steady states of cytochrome ''c'' reduction and energy metabolism. Biochim Biophys Acta 347: 340-358. - Glutamate+succinate as respiratory substrate combination, without comparison of flux with different substrates.<br />
# Tonkonogi M, Walsh B, Tiivel T, Saks V, Sahlin K (1999) Mitochondrial funciton in human skeletal muscle is not impaired by high intensity exercise. Eur J Physiol 437: 562-568.<br />
# Torres NV, Mateo F, Sicilia J, Meléndez-Hevia E. (1988) Distribution of the flux control in convergent metabolic pathways: theory and application to experimental and simulated systems. Int J Biochem 20: 161-165.<br />
# Villani G, Attardi G (1997) In vivo control of respiration by cytochrome c oxidase in wild-type and mitochondrial DNA mutation-carrying human cells. Proc Natl Acad Sci U S A 94: 1166-1171.<br />
# Villani G, Greco M, Papa S, Attardi G (1998) Low reserve capacity of cytochrome c oxidase capacity in vivo in the respiratory chain of a variety of human cell types. J Biol Chem 273: 31829-31836.<br />
# [[Votion 2012 PLoS One|Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890.]] - SUIT protocols.<br />
# Wiedemann FR, Winkler K, Kuznetsov AV, Bartels C, Vielhaber S, Feistner H, Kunz WS (1998) Impairment of mitochondrial function in skeletal muscle of patients with amyotrophic lateral sclerosis. J Neurol Sci 156: 65-72. - - OXPHOS with glutamate+malate is identical or 10% higher than with pyruvate+malate.<br />
# Wilson DF, Rumsey WL, Green TJ, Vanderkooi J (1988). The oxygen dependence of mitochondrial oxidative phosphorylation measured by a new optical method for measuring oxygen concentration. J Biol Chem 263: 2712-2718. - Glutamate+succinate as respiratory substrate combination, without comparison of flux with different substrates.<br />
# Zoccarato F, Cavallini L, Bortolami S, Alexandre A (2007) Succinate modulation of H2O2 release at NADH:ubiquinone oxidoreductase (complex I) in brain mitochondria. Biochem J 406: 125–129. - CI+II substrate combination.<br />
<br />
:>> [[O2k-Publications:_Q-Junction_Effect]]<br />
<br />
=== Notes CI+II ===<br />
# Identical GM<sub>''P''</sub>/GS<sub>''P''</sub> or GM<sub>''P''</sub>/GMS<sub>''P''</sub> ratios of 0.7 are reported for isolated mitochondria (Rasmussen and Rasmussen 2000; Capel et al 2005) and permeabilized fibres (Kunz et al 2000). For a review see Gnaiger (2009).<br />
* '''Correction''': Occasionally 'electron gating' was erroneously spelled as 'gaiting': p. 32; legends to Fig. 1.5 (p. 15) and 4.2 (p. 39).<br />
<br />
== Chapter 5. Respiratory states, coupling control and coupling control ratios ==<br />
<br />
=== References Respiratory states ===<br />
# Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. I. Kinetics of oxygen utilization. J Biol Chem 217: 383-393.<br />
# Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. III. The steady state. J Biol Chem 217: 409-427.<br />
# Chance B, Williams GR (1956) The respiratory chain and oxidative phosphorylation. Adv Enzymol17: 65-134.<br />
# Estabrook R (1967) Mitochondrial respiratory control and the polarographic measurement of ADP:O ratios. Meth Enzymol 10: 41-47.<br />
# [[Gnaiger_2001_Respir Physiol|Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128: 277-297.]]<br />
# [[Gnaiger_1998_Biochim Biophys Acta|Gnaiger E, Lassnig B, Kuznetsov AV, Margreiter R (1998) Mitochondrial respiration in the low oxygen environment of the cell: Effect of ADP on oxygen kinetics. Biochim Biophys Acta 1365: 249-254.]] - Oxygen kinetics is different in the LEAK state without adenylates (''L''<sub>N</sub>) and State 4 (LEAK state with ATP, ''L''<sub>N</sub>).<br />
# [[Gnaiger 2000 Proc Natl Acad Sci U S A|Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97: 11080-11085.]]<br />
# König T, Nicholls DG, Garland PB (1969) The inhibition of pyruvate and Ls(+)-isocitrate oxidation by succinate oxidation in rat liver mitochondria. Biochem J 114: 589-596. - 3½ has been suggested to indicate an intermediate mitochondrial energy state somewhere between States 3 and 4. Would, therefore, State 4 be considered as being somewhere between State 3 and 5?<br />
# Nicholls DG, Ferguson SJ (2002) Bioenergetics 3, Academic Press, London. 287 pp. - ''Misconception on State 2.''<br />
<br />
:>> [[MitoPedia Glossary: Respiratory states]]<br />
<br />
== Chapter 6. Conversions of metabolic fluxes ==<br />
<br />
=== References Conversions ===<br />
<br />
<br />
# Brooks GA, Hittelman KJ, Faulkner JA, Beyer RE (1971) Temperature, skeletal muscle mitochondrial functions, and oxygen debt. Am J Physiol 220: 1053-1059.<br />
# Gnaiger E (1983) Symbols and units: Toward standardization. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York: 352-358.<br />
# Slater EC, Rosing J, Mol A (1973) The phosphorylation potential generated by respiring mitochondria. Biochim Biophys Acta 292: 534-553.<br />
<br />
<br />
<br />
== Apendix: Abbreviations ==<br />
<br />
=== A1. Respiratory coupling states and coupling control ratios ===<br />
<br />
=== A2. Substrates, uncouplers and inhibitors ===<br />
<br />
:>> [[List of substrates and metabolites]]<br />
:>> [[List of uncouplers]]<br />
:>> [[List of inhibitors]]<br />
<br />
<br />
== Follow-up ==<br />
<br />
* The 2007 Edition is cited in: <br />
:# [[Muller 2008 Biochem J|Muller FL et al (2008) High rates of superoxide production in skeletal-muscle mitochondria respiring on both Complex I- and Complex II-linked substrates. Biochem J 409: 491–499.]]<br />
:# [[Droese_2009_Biochim_Biophys_Acta|Dröse S, Hanley PJ, Brandt U (2009) Ambivalent effects of diazoxide on mitochondrial ROS production at respiratory chain complexes I and III. Biochim Biophys Acta 1790: 558-565.]]<br />
:# [[Hickey_2009_Am_J_Physiol_Cell_Physiol|Hickey AJ, Chai CC, Choong SY, de Freitas Costa S, Skea GL, Phillips AR, Cooper GJ (2009) Impaired ATP turnover and ADP supply depress cardiac mitochondrial respiration and elevate superoxide in nonfailing spontaneously hypertensive rat hearts. Am J Physiol Cell Physiol 297: C766–C774.]]<br />
:# Hoffman DL, Brookes PS (2009) Oxygen sensitivity of mitochondrial reactive oxygen species generation depends on metabolic conditions. J Biol Chem 284: 16236-16245.<br />
:# Sahlin K (2009) Control of lipid oxidation at the mitochondrial level. Appl Physiol Nutr Metab 34: 382-388:<br />
:# Bazil JN, Buzzard GT, Rundell AE (2010) Modeling mitochondrial bioenergetics with integrated volume dynamics. PLoS Comput Biol. 6: e1000632.<br />
:# Sivitz WI, Yorek MA (2010) Mitochondrial dysfunction in diabetes: from molecular mechanisms to functional significance and therapeutic opportunities. Antioxid Redox Signal 12: 537-577.<br />
:# Sivitz WI (2010) Mitochondrial Dysfunction in Obesity and Diabetes. US Endocrinology 6: 20-27.<br />
:# [[Iftikar 2010 JEMBE|Iftikar F, MacDonald JR, Hickey AJ (2010) Thermal limits of portunid crab heart mitochondria: Could more thermo-stable mitochondria advantage invasive species? JEMBE 395: 232-239.]]<br />
:# [[Mittal 2011 HPB (Oxford)|Mittal A, Hickey AJ, Chai CC, Loveday BP, Thompson N, Dare A, Delahunt B, Cooper GJ, Windsor JA, Phillips AR (2011) Early organ-specific mitochondrial dysfunction of jejunum and lung found in rats with experimental acute pancreatitis. HPB (Oxford) 13: 332-341.]]<br />
:# [[Pichaud 2011 Am J Physiol Regul Integr Comp Physiol|Pichaud N, Ballard JW, Tanguay RM, Blier PU (2011) Thermal sensitivity of mitochondrial functions in permeabilized muscle fibers from two populations of Drosophila simulans with divergent mitotypes. Am J Physiol Regul Integr Comp Physiol 301: R48-R59.]]<br />
:# [[Ye 2011 J Biol Chem|Ye F, Lemieux H, Hoppel CL, Hanson RW, Hakimi P, Croniger CM, Puchowicz M, Anderson VE, Fujioka H, Stavnezer E (2011) Peroxisome proliferator-activated receptor γ (PPARγ) mediates a Ski-induced shift from glycolysis to oxidative energy metabolism. J Biol Chem 286: 40013-40024.]]<br />
:# Cordeiro OD, Silva TS, Alves RN, Costas B, Wulff T, Richard N, de Vareilles M, Conceição LE, Rodrigues PM (2012) Changes in liver proteome expression of Senegalese sole (Solea senegalensis) in response to repeated handling stress. Mar Biotechnol (NY)14: 714-729.<br />
:# Everett MV, Antal CE, Crawford DL (2012) The effect of short-term hypoxic exposure on metabolic gene expression. J Exp Zool A Ecol Genet Physiol 317: 9-23.<br />
:# [[Miquel 2012 PLoS One|Miquel E, Cassina A, Martinez-Palma L, Bolatto C, Trias E, Gandelman M, Radi R, Barbeito L, Cassina P (2012) Modulation of astrocytic mitochondrial function by dichloroacetate improves survival and motor performance in inherited amyotrophic lateral sclerosis. PLoS One 7: e34776.]]<br />
:# [[De Paula Martins 2013 J Bioenerg Biomembr|de Paula Martins R, Glaser V, da Luz Scheffer D, de Paula Ferreira PM, Wannmacher CM, Farina M, de Oliveira PA, Prediger RD, Latini A (2013) Platelet oxygen consumption as a peripheral blood marker of brain energetics in a mouse model of severe neurotoxicity. J Bioenerg Biomembr 45: 449-457.]]<br />
:# [[Perry 2013 Diabetes|Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62: 1041-1053.]]<br />
:# Chu MJJ, Dare AJ, Bartlett ASJR, Phillips ARJ, Hickey AJ (2013) Effect of Hepatic Steatosis on Bioenergetic Function During Hepatic Ischemia-reperfusion: A Systematic Review. The Open Transplantation J 6: 10-32.<br />
:# [[Gispert_2013_Hum_Mol_Genet|Gispert S, Parganlija D, Klinkenberg M, Dröse S, Wittig I, Mittelbronn M, Grzmil P, Koob S, Hamann A, Walter M, Büchel F, Adler T, Hrabé de Angelis M, Busch DH, Zell A, Reichert AS, Brandt U, Osiewacz HD, Jendrach M, Auburger G (2013) Loss of mitochondrial peptidase Clpp leads to infertility, hearing loss plus growth retardation via accumulation of CLPX, mtDNA and inflammatory factors. Hum Mol Genet 22: 4871-4887.]]<br />
:# [[Hirzel 2013 J Recept Signal Transduct Res|Hirzel E, Lindinger PW, Maseneni S, Giese M, Rhein VV, Eckert A, Hoch M, Krähenbühl S, Eberle AN (2013) Differential modulation of ROS signals and other mitochondrial parameters by the antioxidants MitoQ, resveratrol and curcumin in human adipocytes. J Recept Signal Transduct Res 33:304-312.]]<br />
:# [[Cordero-Reyes_2014_J_Mol_Cell_Cardiol|Cordero-Reyes AM, Gupte AA, Youker KA, Loebe M, Hsueh WA, Torre-Amione G, Taegtmeyer H, Hamilton DJ (2014) Freshly isolated mitochondria from failing human hearts exhibit preserved respiratory function. J Mol Cell Cardiol doi: 10.1016/j.yjmcc.2013.12.029.]]<br />
<br />
* The 2010 Edition is cited in: <br />
:# Granados-Castro LF, Rodríguez-Rangel DS, Montaño M, Ramos C, Pedraza-Chaverri J (2013) Wood smoke exposure induces a decrease in respiration parameters and in the activity of respiratory complexes I and IV in lung mitochondria from guinea pigs. Environ Toxicol. doi: 10.1002/tox.21922.<br />
:# Maldonado F, Packard TT, Gómez M (2012) Understanding tetrazolium reduction and the importance of substrates in measuring respiratory electron transport activity. J Exp Marine Biol Ecol 434–435: 110–118.</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=Gnaiger_2020_BEC_MitoPathways&diff=58097Gnaiger 2020 BEC MitoPathways2014-03-19T15:04:58Z<p>Bauer Christiane: </p>
<hr />
<div>{{Publication<br />
|title=Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp. ISBN 978-3-9502399-6-6<br />
|info=[[Media:Gnaiger 2012 Mitochondr Physiol Network MitoPathways.pdf| '''Bioblast pdf''']]; [http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger_2012_MitoPathways]<br />
|authors=Gnaiger E<br />
|year=2012<br />
|journal=Mitochondr Physiol Network<br />
|keywords=Q-junction, Respiratory states, Flux control ratios<br />
|mipnetlab=AT_Innsbruck_Gnaiger E, AT Innsbruck OROBOROS, AT Innsbruck MitoCom<br />
|discipline=Mitochondrial Physiology<br />
}}<br />
{{Labeling<br />
|area=Respiration, Instruments;methods, mt-Biogenesis;mt-density, Comparative MiP;environmental MiP, Exercise physiology;nutrition;life style, mt-Medicine, mt-Awareness<br />
|organism=Human, Mouse<br />
|tissues=Heart, Skeletal muscle<br />
|model cell lines=Fibroblast<br />
|preparations=Permeabilized cells, Permeabilized tissue, Isolated Mitochondria<br />
|topics=Coupling efficiency;uncoupling, Flux control, Threshold;excess capacity<br />
|couplingstates=LEAK, ROUTINE, OXPHOS, ETS<br />
|substratestates=CI, CII, ETF, GpDH, CI+II<br />
|instruments=Oxygraph-2k, Protocol, Theory<br />
|discipline=Mitochondrial Physiology<br />
}}<br />
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[[File:BlueBook openAccess.png|right|300px]]<br />
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__TOC__<br />
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= Bioblast online references and notes =<br />
* '''Preface''' <br />
: '''References Preface''' <br />
# [[Gnaiger 2007 MitoPathways|Gnaiger E ed (2007) Mitochondrial Pathways and Respiratory Control. 1st ed. OROBOROS MiPNet Publications, Innsbruck: 96 pp]]<br />
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:>> [[MiPNet_Reference_Laboratories|WorldWide MiPNet]]<br />
:>> [http://www.oroboros.at/?O2k-Protocols O2k-Protocols]<br />
:>> [[O2k-Publications]]<br />
:>> [[MitoPedia Glossary: Terms and abbreviations]]<br />
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== Chapter 1. OXPHOS analysis ==<br />
=== References OXPHOS analysis ===<br />
# [[Altmann 1894 Verlag Von Veit & Comp | Altmann R (1894) Die Elementarorganismen und ihre Beziehungen zu den Zellen. Zweite vermehrte Auflage. Verlag Von Veit & Comp, Leipzig. 160 pp, 34 Tafeln.]]<br />
# [[Dawson 2005 J Physiol | Dawson KD, Baker DJ, Greenhaff PL, Gibala MJ (2005) An accute decrease in TCA cycle intermediates does not affect aerobic energy delivery in contracting rat skeletal muscle. J Physiol 565: 637-643.]]<br />
# [[Garlid 1993 BTK | Garlid KD, Semrad C, Zinchenko V (1993) Does redox slip contribute significantly to mitochondrial respiration? In: Schuster S, Rigoulet M, Ouhabi R, Mazat J-P (eds) Modern Trends in Biothermokinetics. Plenum Press, New York, London: 287-293.]]<br />
# [[Gibala 1998 Am J Physiol Endocrinol Metab | Gibala 1998 Am J Physiol Endocrinol Metab|Gibala MJ, MacLean DA, Graham TE, Saltin B (1998) Tricarboxylic acid cycle intermediate pool size and estimated cycle flux in human muscle during exercise. Am J Physiol Endocrinol Metab 275: E235-E242.]] - concentrations of TCA cycle intermediates.<br />
# [[Gnaiger 1983 J Exp Zool|Gnaiger E (1983) Heat dissipation and energetic efficiency in animal anoxibiosis. Economy contra power. J Exp Zool 228: 471-490.]]<br />
# [[Gnaiger 1993 Hypoxia|Gnaiger E (1993) Efficiency and power strategies under hypoxia. Is low efficiency at high glycolytic ATP production a paradox? In: Surviving Hypoxia: Mechanisms of Control and Adaptation. Hochachka PW, Lutz PL, Sick T, Rosenthal M, Van den Thillart G (eds) CRC Press, Boca Raton, Ann Arbor, London, Tokyo: 77-109.]] - The Gibbs force of phorphorylation of ADP to ATP is ''F''<sub>ATP</sub> = 52 to 66 kJ/mol ATP under intracellular conditions.<br />
# [[Gnaiger 1993 Pure Appl Chem|Gnaiger E (1993) Nonequilibrium thermodynamics of energy transformations. Pure Appl Chem 65: 1983-2002.]]<br />
# [[Gnaiger 2003 Adv Exp Med Biol|Gnaiger E (2003) Oxygen conformance of cellular respiration. A perspective of mitochondrial physiology. Adv Exp Med Biol 543: 39-55.]]<br />
# [[Gnaiger 2002 Biochem Soc Trans|Gnaiger E, Kuznetsov AV (2002) Mitochondrial respiration at low levels of oxygen and cytochrome c. Biochem Soc Trans 30: 252-258.]]<br />
# [[Gnaiger 2000 Life in the Cold|Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000b) Mitochondria in the cold. In: Life in the Cold. (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York: pp 431-442.]] – MiR05 as the basis of MiR06.<br />
# [[Gnaiger 1998 Biochim Biophys Acta | Gnaiger E, Lassnig B, Kuznetsov AV, Margreiter R (1998) Mitochondrial respiration in the low oxygen environment of the cell: Effect of ADP on oxygen kinetics. Biochim Biophys Acta 1365: 249-254.]]<br />
# [[Gnaiger 1998 J Exp Biol | Gnaiger E, Lassnig B, Kuznetsov AV, Rieger G, Margreiter R (1998) Mitochondrial oxygen affinity, respiratory flux control, and excess capacity of cytochrome c oxidase. J Exp Biol 201: 1129-1139.]]<br />
# [[Gueguen 2005 J Physiol | Gueguen N, Lefaucheur L, Ecolan P, Fillaut M, Herpin P (2005) Ca2+-activated myosin-ATPases, creatine and adenylate kinases regulate mitochondrial function according to myofibre type in rabbit. J Physiol 564: 723-735.]]<br />
# [[Hatefi 1962 J Biol Chem | Hatefi Y, Haavik AG, Fowler LR, Griffiths DE (1962) Studies on the electron transfer system. XLII. Reconstitution of the electron transfer system. J Biol Chem 237: 2661-2669.]]<br />
# [[Kuznetsov 2004 Am J Physiol Heart Circ Physiol | Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome c release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286: H1633–H1641.]] – Cytochrome ‘’c’’ test.<br />
# [[Lemieux 2006 Biochim Biophys Acta | Lemieux H, Garedew A, Blier PU, Tardif J-C, Gnaiger E (2006) Temperature effects on the control and capacity of mitochondrial respiration in permeabilized fibers of the mouse heart. Biochim Biophys Acta, EBEC Short Reports Suppl 14: 201-202.]]<br />
# [[Mitchell 1961 Nature | Mitchell P (1961) Coupling of phosphorylation to electron and hydrogen transfer by a chemi-osmotic type of mechanism. Nature 191: 144-148.]]<br />
# Mitchell P (1966) Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Glynn Research Ltd, Bodmin: 192 pp. - ''The Grey Book'' 1.<br />
# Mitchell P (1968) Chemiosmotic coupling and energy transduction. Glynn Research Ltd, Bodmin: 111 pp. - ''The Grey Book'' 2.<br />
# [[Mitchell 1967 Biochem J | Mitchell P, Moyle J (1967) Respiration-driven proton translocation in rat liver mitochondria. Biochem J 105: 1147-1162.]]<br />
# [[Mootha 1997 Am J Physiol | Mootha VK, Arai AE, Balaban RS (1997) Maximum oxidative phosphorylation capacity of the mammalian heart. Am J Physiol 272: H769-H775.]] – [Pi] <10 mM and [ADP] <0.4 mM limit OXPHOS in isolated heart mitochondria.<br />
# [[Nicholson 2012 Nature | Nicholson JK, Holmes E, Kinross JM, Darzi AW, Takats Z, Lindon JC (2012) Metabolic phenotyping in clinical and surgical environments. Nature 491: 384-392.]]<br />
# [[Owen 2002 J Biol Chem | Owen OE, Kalhan SC, Hanson RW (2002) The key role of anaplerosis and cataplerosis for citric acid cycle function. J Biol Chem 277: 30409-30412.]]<br />
# [[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]] - >90% saturation is reached only >5 mM ADP, yet few studies use such high [ADP] in permeabilized tissues and cells. - Oxygen limitation of respiration below air saturation.<br />
# [[Puchowicz 2004 Mitochondrion | Puchowicz MA, Varnes ME, Cohen BH, Friedman NR, Kerr DS, Hoppel CL (2004) Oxidative phosphorylation analysis: assessing the integrated functional activity of human skeletal muscle mitochondria – case studies. Mitochondrion 4: 377-385.]] - Cytochrome ''c'' test.<br />
# [[Rasmussen 2000 Mol Cell Biochem | Rasmussen UF, Rasmussen HN (2000) Human quadriceps muscle mitochondria: A functional characterization. Mol Cell Biochem 208: 37-44.]] - Cytochrome ''c'' test.<br />
# [[Renner 2003 Biochim Biophys Acta | Renner K , Amberger A, Konwalinka G, Gnaiger E (2003) Changes of mitochondrial respiration, mitochondrial content and cell size after induction of apoptosis in leukemia cells. Biochim Biophys Acta 1642: 115-123.]]<br />
# [[Rossignol 2003 Biochem J | Rossignol R, Faustin B, Rocher C, Malgat M, Mazat JP, Letellier T (2003) Mitochondrial threshold effects. Biochem J 370: 751-762.]]<br />
# [[Saks 1998 Mol Cell Biochem | Saks VA, Veksler VI, Kuznetsov AV, Kay L, Sikk P, Tiivel T, Tranqui L, Olivares J, Winkler K, Wiedemann F, Kunz WS (1998) Permeabilised cell and skinned fiber techniques in studies of mitochondrial function in vivo. Mol Cell Biochem 184: 81-100.]] - The apparent ''K''<sub>m</sub> for ADP increases up to 0.5 mM in some permeabilized muscle fibres.<br />
# [[Territo 2000 Am J Physiol Cell Physiol| Territo PR, Mootha VK, French SA, Balaban RS (2000) Ca<sup>2+</sup> activation of heart mitochondrial oxidative phosphorylation: role of the F0/F1-ATPase. Am J Physiol Cell Physiol 278: C423-C435.]]<br />
<br />
== Chapter 2. Mitochondrial pathways to Complex I: Respiratory substrate control with pyruvate, malate and glutamate ==<br />
<br />
=== References CI ===<br />
# Brandt U (2006) Energy converting NADH:quinone oxidoreductase (Complex I). Annu Rev Biochem 75: 69-92.<br />
# Brewer GJ, Jones TT, Wallimann T, Schlattner U (2004) Higher respiratory rates and improved creatine stimulation in brain mitochondria isolated with antioxidants. Mitochondrion 4: 49-57.<br />
# Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. III. The steady state. J Biol Chem 217: 409-427. - Substrate depletion in isolated mitochondria is achieved in State 2: ADP is added to induce a transient stimulation of oxygen flux based on oxidation of endogenous substrates. <br />
# Digerness SB, Reddy WJ (1976) The malate-aspartate shuttle in heart mitochondria. J Mol Cell Cardiol. 8: 779-785.<br />
# Duchen MR (2004) Roles of mitochondria in health and disease. Diabetes 53, Suppl 1: S96-S102. - Mitochondrial glutamate dehydrogenase is particularly active in astrocytes, preventing glutamate induced neurotoxicity.<br />
# [[Gnaiger 2009 Int J Biochem Cell Biol|Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837–1845.]]<br />
# Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97: 11080-11085. - Equilibrium ratio of malate to fumarate is 4.1.<br />
# Hildyard JCW, Halestrap AP (2003) Identification of the mitochondrial pyruvate carrier in ''Saccharomyces cerevidiae''. Biochem J 374: 607-611.<br />
# Johnson G, Roussel D, Dumas JF, Douay O, Malthiery Y, Simard G, Ritz P (2006) Influence of intensity of food restriction on skeletal muscle mitochondrial energy metabolism in rats. Am J Physiol Endocrinol Metab 291: E460-E467. - Uncoupling stimulates coupled OXPHOS respiration, PM<sub>''P''</sub>, by 14%.<br />
# Kemp RB, Hoare S, Schmalfeldt M, Bridge CM, Evans PM, Gnaiger E (1994) A thermochemical study of the production of lactate by glutaminolysis and glycolysis in mouse macrophage hybridoma cells. In What is Controlling Life? (Gnaiger E, Gellerich FN, Wyss M, eds) Modern Trends in BioThermoKinetics 3, Innsbruck Univ Press: 226-231. - Glutamate derived from hydrolyzation of glutamine is a very important aerobic substrate in cultured cells.<br />
# Lemasters JJ (1984) The ATP-to-oxygen stoichiometries of oxidative phosphorylation by rat liver mitochondria. J Biol Chem 259: 13123-13130. - Malonate added to inhibit the succinate-fumarate reaction exerts only a minor effect on liver mitochondrial respiration.<br />
# Maechler P, Carobbio S, Rubi B (2006) In beta-cells, mitochondria integrate and generate metabolic signals controlling insulin secretion. Int J Biochem Cell Biol 38: 696-709.<br />
# Messer JI, Jackman MR, Willis WT (2004) Pyruvate and citric acid cycle carbon requirements in isolated skeletal muscle mitochondria. Am J Physiol Cell Physiol 286: C565-572. - With malate alone and saturating [ADP] isolated rat skeletal muscle mitochondria respire at only 1.3% of OXPHOS capacity with pyruvate+malate. Pyruvate alone yields only 2.1% of OXPHOS capacity (State P) with PM.<br />
# Nicholls DG, Ferguson SJ (2002) Bioenergetics 3, Academic Press, London. 287 pp. - Carriers.<br />
# Ouhabi R, Boue-Grabot M, Mazat J-P (1994) ATP synthesis in permeabilized cells: Assessment of the ATP/O ratios in situ. In What is Controlling Life? (Gnaiger E, Gellerich FN, Wyss M, eds) Modern Trends in BioThermoKinetics 3, Innsbruck Univ Press: 141-144. - In fibroblasts, GM<sub>''P''</sub> supports a higher respiratory flux than PM<sub>''P''</sub>.<br />
# O’Donnell JM, Kudej RK, LaNoue KF, Vatner SF, Lewandowski ED (2004) Limited transfer of cytosolic NADH into mitochondria at high cardiac workload. Am J Physiol Heart Circ Physiol 286: H2237-H2242.<br />
# Puchowicz MA, Varnes ME, Cohen BH, Friedman NR, Kerr DS, Hoppel CL (2004) Oxidative phosphorylation analysis: assessing the integrated functional activity of human skeletal muscle mitochondria – case studies. Mitochondrion 4: 377-385. - OXPHOS with glutamate alone is 50% to 85% of respiration with glutamate+malate. Accumulation of fumarate inhibits succinate dehydrogenase and glutamate dehydrogenase (Caughey et al 1957; Dervartanian, Veeger 1964). - OXPHOS with glutamate+malate is identical or 10% higher than with pyruvate+malate.<br />
# Rasmussen UF, Rasmussen HN (2000) Human quadriceps muscle mitochondria: A functional characterization. Mol Cell Biochem 208: 37-44. - Uncoupling stimulates coupled OXPHOS respiration, PMP, by 15% in human skeletal muscle. OXPHOS with glutamate alone is 50% to 85% of respiration with glutamate+malate. - OXPHOS with glutamate+malate is identical or 10% higher than with pyruvate+malate.<br />
# Thomas et al (2004) - OXPHOS in human skeletal muscle for PM<sub>''P''</sub> is 25% higher than for GM<sub>''P''</sub>.<br />
# Winkler-Stuck K, Kirches E, Mawrin C, Dietzmann K, Lins H, Wallesch CW, Kunz WS, Wiedemann FR (2005) Re-evaluation of the dysfunction of mitochondrial respiratory chain in skeletal muscle of patients with Parkinson's disease. J Neural Transm 112: 499-518. - OXPHOS in human skeletal muscle for PM<sub>''P''</sub> is 16% higher than for GM<sub>''P''</sub>.<br />
<br />
=== Notes CI ===<br />
# Schwerzmann et al (1989) Proc Natl Acad Sci U S A 86: 1583-1587. “Of the substrates used here, pyruvate/malate activates the chain at complex I, glutamate/malate and succinate at complexes II and III, ..” - This consideration of glutamate+malate requires correction.<br />
# Ponsot et al (2005) J Cell Physiol 203: 479-486. (a) Respiration (State 3) in permeabilized fibres with malate alone gave 25-50% of the flux with pyruvate+malate. This most likely indicates a large content of endogenous mitochondrial substrates, which interfere to an unknown degree with the kinetics of respiration after addition of exogenous substrates. In such a study, the conventional initial depletion of endogenous substrates would be most important. (b) Maximal respiration rates in muscle should be evaluated at saturating or high Pi, since at a Pi concentration of 3 mM OXPHOS respiration is phosphate limited.<br />
# Hulbert et al (2006) J Comp Physiol B 176: 93-105. Addition of ‘sparking malate concentrations’. This term can probably be derived from the misconception that tricarboxylic acid cycle intermediates are conserved during respiration of isolated mitochondria. 380 µM malate (instead of mM concentrations) in conjunction with 2.4 mM pyruvate were used, which makes a comparison difficult between different tissues and different species: the low malate concentration may limit PMP flux at various degrees in the different sources of mitochondria, and GMP may support higher fluxes than PMP at tissue- and species-specific degrees.<br />
<br />
<br />
== Chapter 3. Mitochondrial pathways to Complex II. Glycerophosphate dehydrogenase and electrontransferring flavoprotein ==<br />
<br />
=== References CII ===<br />
# Capel F, Rimbert V, Lioger D, Diot A, Rousset P, Patureau Mirand P, Boirie Y, Morio B, Mosoni L (2005) Due to reverse electron transfer, mitochondrial H2O2 release increases with age in human vastus lateralis muscle although oxidative capacity is preserved. Mech Ageing Develop 126: 505-511. - With succinate alone OXPHOS is 30-40% lower than with succinate+rotenone in human skeletal muscle mitochondria.<br />
# Cecchini G (2003) Function and structure of Complex II of the respiratory chain. Annu Rev Biochem 72: 77-109.<br />
# Ernster L, Nordenbrand K (1967) Skeletal muscle mitochondria. In: Estabrook RW, Pullman ME (eds) Meth Enzymol: 86-94. – With succinate alone OXPHOS is 30-40% lower than with succinate+rotenone in rat skeletal muscle mitochondria.<br />
# Jackman MR, Willis WT (1996) Characteristics of mitochondria isolated from type I and type IIb skeletal muscle. Am J Physiol Cell Physiol 270: C673-678. - Glycerophosphate oxidation is 10-fold higher in rabbit gracilis mitochondria compared to soleus.<br />
# Lehninger AL (1970) Biochemistry. The molecular basis of cell structure and function Worth. 833 pp. - Oxaloacetate is a more potent competitive inhibitor of succinate dehydrogenase than malonate even at small concentration (p. 352).<br />
# Muller FL, Liu Y, Abdul-Ghani MA, Lustgarten MS, Bhattacharya A, Jang YC, Van Remmen H (2008) High rates of superoxide production in skeletal-muscle mitochondria respiring on both Complex I- and Complex II-linked substrates. Biochem J 409: 491–499. - Addition of malate inhibits superoxide production with succinate, probably due to the oxaloacetate inhibition of CII.<br />
# Rauchova H, Drahota Z, Rauch P, Fato R, Lenaz G (2003) Coenzyme Q releases the inhibitory effect of free fatty acids on mitochondrial glycerophosphate dehydrogenase. Acta Biochim Polonica 50: 405-413. - Glycerophosphate is an important substrate for respiration in brown adipose tissue mitochondria.<br />
# Rasmussen UF, Rasmussen HN (2000) Human quadriceps muscle mitochondria: A functional characterization. Mol Cell Biochem 208: 37-44. – Glycerophosphate oxidation is relatively slow.<br />
# Sun F, Huo X, Zhai Y, Wang A, Xu J, Su D, Bartlam M, Rao Z (2005) Crystal structure of mitochondrial respiratory membrane protein Complex II. Cell 121: 1043–1057.<br />
<br />
=== Notes CII === <br />
# Ponsot et al (2005) J Cell Physiol 203: 479-486. ‘.. the mitochondrial form of GPDH, which produces FADH2 within the mitochondrial matrix and provides electrons to Compoex II of the phosphorylation chain’. – The mitochondrial glycerophosphate dehydrogenase (GpDH), located on the outer side of the inner mitochondrial membrane, does not provide electrons to CII, but feeds electrons into the Q-cycle entirely independent of CII. FADH2 is not produced within the mitochondrial matrix. Electron transfer takes place from the mitochondrial inner membrane flavoprotein-linked glycerophosphate dehydrogenase to CoQ.<br />
# In the first edition of ‘Mitochondrial Pathways and Respiratory Control’ (2007), the term ‘electron transport’ is used synonymously for ‘electron transfer’.<br />
<br />
<br />
<br />
== Chapter 4. Mitochondrial pathways to Complexes I+II: Convergent electron transfer at the Q-Junction and additive effect of substrate combinations ==<br />
<br />
=== References CI+II ===<br />
# Aragonés J, Schneider M, Van Geyte K, Fraisl P, Dresselaers T, Mazzone M, Dirkx R, Zacchigna S, Lemieux H, Jeoung NH, Lambrechts D, Bishop T, Lafuste P, Diez-Juan A, K Harten S, Van Noten P, De Bock K, Willam C, Tjwa M, Grosfeld A, Navet R, Moons L, Vandendriessche T, Deroose C, Wijeyekoon B, Nuyts J, Jordan B, Silasi-Mansat R, Lupu F, Dewerchin M, Pugh C, Salmon P, Mortelmans L, Gallez B, Gorus F, Buyse J, Sluse F, Harris RA, Gnaiger E, Hespel P, Van Hecke P, Schuit F, Van Veldhoven P, Ratcliffe P, Baes M, Maxwell P, Carmeliet P (2008) Deficiency or inhibition of oxygen sensor Phd1 induces hypoxia tolerance by reprogramming basal metabolism. Nat Genet 40: 170-180. - OXPHOS analysis for phenotyping.<br />
# Bianchi C, Genova ML, Parenti Castelli G, Lenaz G (2004) The mitochondrial respiratory chain is partially organized in a supercomplex assembly: kinetic evidence using flux control analysis. J Biol Chem 279: 36562-36569.<br />
# Boushel R, Gnaiger E, Schjerling P, Skovbro M, Kraunsoe R, Flemming D (2007) Patients with Type 2 Diabetes have normal mitochondrial function in skeletal muscle. Diabetologia 50: 790-796.<br />
# Boushel R, Gnaiger E, Calbet JA, Gonzalez-Alonso J, Wright-Paradis C, Sondergaard H, Ara I, Helge JW, Saltin B (2011) Muscle mitochondrial capacity exceeds maximal oxygen delivery in humans. Mitochondrion 11: 303-307.<br />
# Capel F, Rimbert V, Lioger D, Diot A, Rousset P, Patureau Mirand P, Boirie Y, Morio B, Mosoni L (2005) Due to reverse electron transfer, mitochondrial H2O2 release increases with age in human vastus lateralis muscle although oxidative capacity is preserved. Mech Ageing Develop 126: 505-511. - CI+II substrate combination.<br />
# Chance B (1965) Reaction of oxygen with the respiratory chain in cells and tissues. J Gen Physiol 49: 163-188. - Glutamate+succinate as respiratory substrate combination, without comparison of flux with different substrates.<br />
# Costa LE, Boveris A, Koch OR, Taquini AC (1988) Liver and heart mitochondria in rats submitted to chronic hypobaric hypoxia. Am J Physiol Cell Physiol 255: C123-C129.<br />
# Digerness SB, Reddy WJ (1976) The malate-aspartate shuttle in heart mitochondria. J Mol Cell Cardiol. 8: 779-785.<br />
# Eberhart K, Rainer J, Bindreither D, Ritter I, Gnaiger E, Kofler R, Oefner PJ, Renner K (2011) Glucocorticoid-induced alterations in mitochondrial membrane properties and respiration in childhood acute lymphoblastic leukemia. Biochim Biophys Acta 1807: 719-725.<br />
# Estabrook R (1967) Mitochondrial respiratory control and the polarographic measurement of ADP:O ratios. Meth Enzymol 10: 41-47.<br />
# Garait B, Couturier K, Servais S, Letexier D, Perrin D, Batandier C, Rouanet J-L, Sibille B, Rey B, Leverve X, Favier R (2005) Fat intake reverses the beneficial effects of low caloric intake on skeletal muscle mitochondrial H2O2 production. Free Radic Biol Med 39: 1249–1261. - GM<sub>''P''</sub>/GMS<sub>''P''</sub> substrate control ratio in skeletal muscle of rats fed on various diets ranges from 0.7 to 0.8. <br />
# Garedew A, Lemieux H, Schachner T, Blier PU, Tardif J-C, Gnaiger E (2006) High excess capacity of cytochrome c oxidase in permeabilized fibers of the mouse heart. Biochim Biophys Acta, EBEC Short Reports Suppl 14: 167-168.<br />
# [[Gnaiger 2009 Int J Biochem Cell Biol|Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837–1845.]]<br />
# Gnaiger E, Wright-Paradis C, Sondergaard H, Lundby C, Calbet JA, Saltin B, Helge J, Boushel R (2005) High-resolution respirometry in small biopsies of human muscle: correlations with body mass index and age. Mitochondr Physiol Network 10.9: 14-15. http://www.mitophysiology.org/?gnaigere.<br />
# González-Flecha B, Cutrin JC, Boveris A (1993) Time course and mechanism of oxidative stress and tissue damage in rat liver subjected to in vivo ischemia-reperfusion. J Clin Invest 91: 456-464. - Respiration was measured in states GS<sub>''P''</sub> and GM<sub>''P''</sub>.<br />
# Gutman M, Coles CJ, Singer TP, Casida JE (1971) On the functional organization of the respiratory chain at the dehydrogenase-coenzyme Q junction. Biochemistry 10: 2036-2043.<br />
# Hansford RG, Hogue BA, Mildaziene V (1997) Dependence of H2O2 formation by rat heart mitochondria on substrate availability and donor age. J Bioenerg Biomembr 29: 89–95.<br />
# Hatefi Y, Haavik AG, Fowler LR, Griffiths DE (1962) Studies on the electron transfer system. XLII. Reconstitution of the electron transfer system. J Biol Chem 237: 2661-2669.<br />
# König T, Nicholls DG, Garland PB (1969) The inhibition of pyruvate and Ls(+)-isocitrate oxidation by succinate oxidation in rat liver mitochondria. Biochem J 114: 589-596.<br />
# Krebs HA (1935) CXCVII. Metabolism of amino-acids. III. Deamination of amino-acids. Biochem J 29: 1620-1644.<br />
# Kunz WS, Kudin A, Vielhaber S, Elger CE, Attardi G, Villani G (2000) Flux control of cytochrome c oxidase in human skeletal muscle. J Biol Chem 275: 27741-27745.<br />
# Kuznetsov AV, Clark JF, Winkler K, Kunz WS (1996) Increase of flux control of cytochrome c oxidase in copper-deficient mottled brindled mice. J Biol Chem 271: 283-288.<br />
# Kuznetsov AV, Strobl D, Ruttmann E, Königsrainer A, Margreiter R, Gnaiger E (2002) Evaluation of mitochondrial respiratory function in small biopsies of liver. Analyt Biochem 305: 186-194. - S(Rot) alone supports a higher flux than GM in liver mitocondria.<br />
# Kuznetsov AV, Winkler K, Kirches E, Lins H, Feistner H, Kunz WS (1997) Application of inhibitor titrations for the detection of oxidative phosphorylation defects in saponin-skinned muscle fibers of patients with mitochondrial diseases. Biochim Biophys Acta 1360: 142-150. - OXPHOS with glutamate+malate is identical or 10% higher than with pyruvate+malate.<br />
# LaNoue KF, Bryla J, Williamson JR (1972) Feedback interactions in the control of citric acid cycle activity in rat heart mitochondria. J Biol Chem 247: 667-679.<br />
# LaNoue KF, Schoolwerth AC (1979) Metabolite transport in mitochondria. Annu Rev Biochem 48: 871-922.<br />
# Lehninger AL (1970) Biochemistry. The molecular basis of cell structure and function Worth. 833 pp. - Electron transport chain.<br />
# Lemieux H, Garedew A, Blier PU, Tardif J-C, Gnaiger E (2006) Temperature effects on the control and capacity of mitochondrial respiration in permeabilized fibers of the mouse heart. Biochim Biophys Acta, EBEC Short Reports Suppl 14: 201-202.<br />
# [[Lemieux 2011 Int J Biochem Cell Biol|Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43: 1729–1738.]] - SUIT protocols.<br />
# Lenaz G, Genova ML (2009) Structural and functional organization of the mitochondrial respiratory chain: A dynamic super-assembly. Int J Biochem Cell Biol 41: 1750-1772.<br />
# Llesuy S, Evelson P, González-Flecha B, Peralta J, Carreras MC, Poderoso JJ, Boveris A (1994) Oxidative stress in muscle and liver of rats with septic syndrome. Free Radic Biol Med 16: 445-451. - GM<sub>''P''</sub>/GS<sub>''P''</sub> substrate control ratios are 0.8 and 0.7 in liver mitochondria (male Wistar and female Sprague-Dawley, respectively).<br />
# Mogensen M, Sahlin K (2005) Mitochondrial efficiency in rat skeletal muscle: influence of respiration rate, substrate and muscle type. Acta Physiol Scand 185: 229-236. - The flux control ratio of palmitoylcarnitine+malate/PM (OXPHOS) is 0.6 for mitochondria isolated from rat extensor digitorum longus muscle (mainly type II fibre type), but is 0.95 in rat soleus muscle (type I fibre type).<br />
# Muller FL, Liu Y, Abdul-Ghani MA, Lustgarten MS, Bhattacharya A, Jang YC, Van Remmen H (2008) High rates of superoxide production in skeletal-muscle mitochondria respiring on both Complex I- and Complex II-linked substrates. Biochem J 409: 491–499.<br />
# Nicholls DG, Ferguson SJ (2002) Bioenergetics 3, Academic Press, London. 287 pp. - Electron transport chain.<br />
# [[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]] - SUIT protocols.<br />
# [[Pesta 2011 Am J Physiol Regul Integr Comp Physiol|Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.]] - SUIT protocols.<br />
# Rasmussen HN, Rasmussen UF (1997) Small scale preparation of skeletal muscle mitochondria, criteria for integrity, and assays with reference to tissue function. Mol Cell Biochem 174: 55-60.<br />
# Rasmussen UF, Rasmussen HN (2000) Human quadriceps muscle mitochondria: A functional characterization. Mol Cell Biochem 208: 37-44. - OXPHOS with malate+palmitoylcarnitine is 0.60 of OXPHOS with GM.<br />
# Rasmussen UF, Rasmussen HN, Krustrup P, Quistorff B, Saltin B, Bangsbo J (2001) Aerobic metabolism of human quadriceps muscle: in vivo data parallel measurements on isolated mitochondria. Am J Physiol Endocrinol Metab 280: E301–E307.<br />
# Rossignol R, Faustin B, Rocher C, Malgat M, Mazat JP, Letellier T (2003) Mitochondrial threshold effects. Biochem J 370: 751-762.<br />
# Rumsey WL, Schlosser C, Nuutinen EM, Robiolio M, Wilson DF (1990) Cellular energetics and the oxygen dependence of respiration in cardiac myocytes isolated from adult rat. J Biol Chem 265. 15392-15399. - 2.5-fold increase of respiration in the LEAK state with GMS compared to GM.<br />
# Schägger H, Pfeiffer K (2001) The ratio of oxidative phosphorylation complexes I-V in bovine heart mitochondria and the composition of respiratory chain supercomplexes. J Biol Chem 276: 37861-37867.<br />
# Short KR, Bigelow ML, Kahl J, Singh R, Coenen-Schimke J, Raghavakaimal S, Nair KS (2005) Decline in skeletal muscle mitochondrial function with aging in humans. Proc Natl Acad Sci U S A 102: 5618 - 5623.<br />
# Simon J, van Spanning RJM, Richardson DJ (2008) The organisation of proton motive and non-proton motive redox loops in prokaryotic respiratory systems. Biochim Biophys Acta 1777: 1480–1490.<br />
# Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271: C2053-C2061.<br />
# Steinlechner-Maran R, Eberl T, Kunc M, Schröcksnadel H, Margreiter R, Gnaiger E (1997) Respiratory defect as an early event in preservation/reoxygenation injury in endothelial cells. Transplantation 63: 136-142.<br />
# Stadlmann S, Rieger G, Amberger A, Kuznetsov AV, Margreiter R, Gnaiger E (2002) H2O2-mediated oxidative stress versus cold ischemia-reperfusion: mitochondrial respiratory defects in cultured human endothelial cells. Transplantation 74: 1800-1803.<br />
# Sugano T, Oshino N, Chance B (1974). Mitochondrial functions under hypoxic conditions. The steady states of cytochrome ''c'' reduction and energy metabolism. Biochim Biophys Acta 347: 340-358. - Glutamate+succinate as respiratory substrate combination, without comparison of flux with different substrates.<br />
# Tonkonogi M, Walsh B, Tiivel T, Saks V, Sahlin K (1999) Mitochondrial funciton in human skeletal muscle is not impaired by high intensity exercise. Eur J Physiol 437: 562-568.<br />
# Torres NV, Mateo F, Sicilia J, Meléndez-Hevia E. (1988) Distribution of the flux control in convergent metabolic pathways: theory and application to experimental and simulated systems. Int J Biochem 20: 161-165.<br />
# Villani G, Attardi G (1997) In vivo control of respiration by cytochrome c oxidase in wild-type and mitochondrial DNA mutation-carrying human cells. Proc Natl Acad Sci U S A 94: 1166-1171.<br />
# Villani G, Greco M, Papa S, Attardi G (1998) Low reserve capacity of cytochrome c oxidase capacity in vivo in the respiratory chain of a variety of human cell types. J Biol Chem 273: 31829-31836.<br />
# [[Votion 2012 PLoS One|Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890.]] - SUIT protocols.<br />
# Wiedemann FR, Winkler K, Kuznetsov AV, Bartels C, Vielhaber S, Feistner H, Kunz WS (1998) Impairment of mitochondrial function in skeletal muscle of patients with amyotrophic lateral sclerosis. J Neurol Sci 156: 65-72. - - OXPHOS with glutamate+malate is identical or 10% higher than with pyruvate+malate.<br />
# Wilson DF, Rumsey WL, Green TJ, Vanderkooi J (1988). The oxygen dependence of mitochondrial oxidative phosphorylation measured by a new optical method for measuring oxygen concentration. J Biol Chem 263: 2712-2718. - Glutamate+succinate as respiratory substrate combination, without comparison of flux with different substrates.<br />
# Zoccarato F, Cavallini L, Bortolami S, Alexandre A (2007) Succinate modulation of H2O2 release at NADH:ubiquinone oxidoreductase (complex I) in brain mitochondria. Biochem J 406: 125–129. - CI+II substrate combination.<br />
<br />
:>> [[O2k-Publications:_Q-Junction_Effect]]<br />
<br />
=== Notes CI+II ===<br />
# Identical GM<sub>''P''</sub>/GS<sub>''P''</sub> or GM<sub>''P''</sub>/GMS<sub>''P''</sub> ratios of 0.7 are reported for isolated mitochondria (Rasmussen and Rasmussen 2000; Capel et al 2005) and permeabilized fibres (Kunz et al 2000). For a review see Gnaiger (2009).<br />
* '''Correction''': Occasionally 'electron gating' was erroneously spelled as 'gaiting': p. 32; legends to Fig. 1.5 (p. 15) and 4.2 (p. 39).<br />
<br />
== Chapter 5. Respiratory states, coupling control and coupling control ratios ==<br />
<br />
=== References Respiratory states ===<br />
# Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. I. Kinetics of oxygen utilization. J Biol Chem 217: 383-393.<br />
# Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. III. The steady state. J Biol Chem 217: 409-427.<br />
# Chance B, Williams GR (1956) The respiratory chain and oxidative phosphorylation. Adv Enzymol17: 65-134.<br />
# Estabrook R (1967) Mitochondrial respiratory control and the polarographic measurement of ADP:O ratios. Meth Enzymol 10: 41-47.<br />
# [[Gnaiger_2001_Respir Physiol|Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128: 277-297.]]<br />
# [[Gnaiger_1998_Biochim Biophys Acta|Gnaiger E, Lassnig B, Kuznetsov AV, Margreiter R (1998) Mitochondrial respiration in the low oxygen environment of the cell: Effect of ADP on oxygen kinetics. Biochim Biophys Acta 1365: 249-254.]] - Oxygen kinetics is different in the LEAK state without adenylates (''L''<sub>N</sub>) and State 4 (LEAK state with ATP, ''L''<sub>N</sub>).<br />
# [[Gnaiger 2000 Proc Natl Acad Sci U S A|Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97: 11080-11085.]]<br />
# König T, Nicholls DG, Garland PB (1969) The inhibition of pyruvate and Ls(+)-isocitrate oxidation by succinate oxidation in rat liver mitochondria. Biochem J 114: 589-596. - 3½ has been suggested to indicate an intermediate mitochondrial energy state somewhere between States 3 and 4. Would, therefore, State 4 be considered as being somewhere between State 3 and 5?<br />
# Nicholls DG, Ferguson SJ (2002) Bioenergetics 3, Academic Press, London. 287 pp. - ''Misconception on State 2.''<br />
<br />
:>> [[MitoPedia Glossary: Respiratory states]]<br />
<br />
== Chapter 6. Conversions of metabolic fluxes ==<br />
<br />
=== References Conversions ===<br />
<br />
<br />
# Brooks GA, Hittelman KJ, Faulkner JA, Beyer RE (1971) Temperature, skeletal muscle mitochondrial functions, and oxygen debt. Am J Physiol 220: 1053-1059.<br />
# Gnaiger E (1983) Symbols and units: Toward standardization. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York: 352-358.<br />
# Slater EC, Rosing J, Mol A (1973) The phosphorylation potential generated by respiring mitochondria. Biochim Biophys Acta 292: 534-553.<br />
<br />
<br />
<br />
== Apendix: Abbreviations ==<br />
<br />
=== A1. Respiratory coupling states and coupling control ratios ===<br />
<br />
=== A2. Substrates, uncouplers and inhibitors ===<br />
<br />
:>> [[List of substrates and metabolites]]<br />
:>> [[List of uncouplers]]<br />
:>> [[List of inhibitors]]<br />
<br />
<br />
== Follow-up ==<br />
<br />
* The 2007 Edition is cited in: <br />
:# [[Muller 2008 Biochem J|Muller FL et al (2008) High rates of superoxide production in skeletal-muscle mitochondria respiring on both Complex I- and Complex II-linked substrates. Biochem J 409: 491–499.]]<br />
:# [[Droese_2009_Biochim_Biophys_Acta|Dröse S, Hanley PJ, Brandt U (2009) Ambivalent effects of diazoxide on mitochondrial ROS production at respiratory chain complexes I and III. Biochim Biophys Acta 1790: 558-565.]]<br />
:# [[Hickey_2009_Am_J_Physiol_Cell_Physiol|Hickey AJ, Chai CC, Choong SY, de Freitas Costa S, Skea GL, Phillips AR, Cooper GJ (2009) Impaired ATP turnover and ADP supply depress cardiac mitochondrial respiration and elevate superoxide in nonfailing spontaneously hypertensive rat hearts. Am J Physiol Cell Physiol 297: C766–C774.]]<br />
:# Hoffman DL, Brookes PS (2009) Oxygen sensitivity of mitochondrial reactive oxygen species generation depends on metabolic conditions. J Biol Chem 284: 16236-16245.<br />
:# Sahlin K (2009) Control of lipid oxidation at the mitochondrial level. Appl Physiol Nutr Metab 34: 382-388:<br />
:# Bazil JN, Buzzard GT, Rundell AE (2010) Modeling mitochondrial bioenergetics with integrated volume dynamics. PLoS Comput Biol. 6: e1000632.<br />
:# Sivitz WI, Yorek MA (2010) Mitochondrial dysfunction in diabetes: from molecular mechanisms to functional significance and therapeutic opportunities. Antioxid Redox Signal 12: 537-577.<br />
:# Sivitz WI (2010) Mitochondrial Dysfunction in Obesity and Diabetes. US Endocrinology 6: 20-27.<br />
:# [[Iftikar 2010 JEMBE|Iftikar F, MacDonald JR, Hickey AJ (2010) Thermal limits of portunid crab heart mitochondria: Could more thermo-stable mitochondria advantage invasive species? JEMBE 395: 232-239.]]<br />
:# [[Mittal 2011 HPB (Oxford)|Mittal A, Hickey AJ, Chai CC, Loveday BP, Thompson N, Dare A, Delahunt B, Cooper GJ, Windsor JA, Phillips AR (2011) Early organ-specific mitochondrial dysfunction of jejunum and lung found in rats with experimental acute pancreatitis. HPB (Oxford) 13: 332-341.]]<br />
:# [[Pichaud 2011 Am J Physiol Regul Integr Comp Physiol|Pichaud N, Ballard JW, Tanguay RM, Blier PU (2011) Thermal sensitivity of mitochondrial functions in permeabilized muscle fibers from two populations of Drosophila simulans with divergent mitotypes. Am J Physiol Regul Integr Comp Physiol 301: R48-R59.]]<br />
:# [[Ye 2011 J Biol Chem|Ye F, Lemieux H, Hoppel CL, Hanson RW, Hakimi P, Croniger CM, Puchowicz M, Anderson VE, Fujioka H, Stavnezer E (2011) Peroxisome proliferator-activated receptor γ (PPARγ) mediates a Ski-induced shift from glycolysis to oxidative energy metabolism. J Biol Chem 286: 40013-40024.]]<br />
:# Cordeiro OD, Silva TS, Alves RN, Costas B, Wulff T, Richard N, de Vareilles M, Conceição LE, Rodrigues PM (2012) Changes in liver proteome expression of Senegalese sole (Solea senegalensis) in response to repeated handling stress. Mar Biotechnol (NY)14: 714-729.<br />
:# Everett MV, Antal CE, Crawford DL (2012) The effect of short-term hypoxic exposure on metabolic gene expression. J Exp Zool A Ecol Genet Physiol 317: 9-23.<br />
:# [[Miquel 2012 PLoS One|Miquel E, Cassina A, Martinez-Palma L, Bolatto C, Trias E, Gandelman M, Radi R, Barbeito L, Cassina P (2012) Modulation of astrocytic mitochondrial function by dichloroacetate improves survival and motor performance in inherited amyotrophic lateral sclerosis. PLoS One 7: e34776.]]<br />
:# [[De Paula Martins 2013 J Bioenerg Biomembr|de Paula Martins R, Glaser V, da Luz Scheffer D, de Paula Ferreira PM, Wannmacher CM, Farina M, de Oliveira PA, Prediger RD, Latini A (2013) Platelet oxygen consumption as a peripheral blood marker of brain energetics in a mouse model of severe neurotoxicity. J Bioenerg Biomembr 45: 449-457.]]<br />
:# [[Perry 2013 Diabetes|Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62: 1041-1053.]]<br />
:# Chu MJJ, Dare AJ, Bartlett ASJR, Phillips ARJ, Hickey AJ (2013) Effect of Hepatic Steatosis on Bioenergetic Function During Hepatic Ischemia-reperfusion: A Systematic Review. The Open Transplantation J 6: 10-32.<br />
:# [[Gispert_2013_Hum_Mol_Genet|Gispert S, Parganlija D, Klinkenberg M, Dröse S, Wittig I, Mittelbronn M, Grzmil P, Koob S, Hamann A, Walter M, Büchel F, Adler T, Hrabé de Angelis M, Busch DH, Zell A, Reichert AS, Brandt U, Osiewacz HD, Jendrach M, Auburger G (2013) Loss of mitochondrial peptidase Clpp leads to infertility, hearing loss plus growth retardation via accumulation of CLPX, mtDNA and inflammatory factors. Hum Mol Genet 22: 4871-4887.]]<br />
:# [[Hirzel 2013 J Recept Signal Transduct Res|Hirzel E, Lindinger PW, Maseneni S, Giese M, Rhein VV, Eckert A, Hoch M, Krähenbühl S, Eberle AN (2013) Differential modulation of ROS signals and other mitochondrial parameters by the antioxidants MitoQ, resveratrol and curcumin in human adipocytes. J Recept Signal Transduct Res 33:304-312.]]<br />
:# [[Cordero-Reyes_2014_J_Mol_Cell_Cardiol|Cordero-Reyes AM, Gupte AA, Youker KA, Loebe M, Hsueh WA, Torre-Amione G, Taegtmeyer H, Hamilton DJ (2014) Freshly isolated mitochondria from failing human hearts exhibit preserved respiratory function. J Mol Cell Cardiol doi: 10.1016/j.yjmcc.2013.12.029.]]<br />
<br />
* The 2010 Edition is cited in: <br />
:# Granados-Castro LF, Rodríguez-Rangel DS, Montaño M, Ramos C, Pedraza-Chaverri J (2013) Wood smoke exposure induces a decrease in respiration parameters and in the activity of respiratory complexes I and IV in lung mitochondria from guinea pigs. Environ Toxicol. doi: 10.1002/tox.21922.<br />
:# Maldonado F, Packard TT, Gómez M (2012) Understanding tetrazolium reduction and the importance of substrates in measuring respiratory electron transport activity. J Exp Marine Biol Ecol 434–435: 110–118.</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=Gnaiger_2020_BEC_MitoPathways&diff=58096Gnaiger 2020 BEC MitoPathways2014-03-19T15:04:40Z<p>Bauer Christiane: </p>
<hr />
<div>{{Publication<br />
|title=Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp. ISBN 978-3-9502399-6-6<br />
|info=[[Media:Gnaiger 2012 Mitochondr Physiol Network MitoPathways.pdf| '''Bioblast pdf''']]; [http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger_2012_MitoPathways]<br />
|authors=Gnaiger E<br />
|year=2012<br />
|journal=Mitochondr Physiol Network<br />
|keywords=Q-junction, Respiratory states, Flux control ratios<br />
|mipnetlab=AT_Innsbruck_Gnaiger E, AT Innsbruck OROBOROS, AT Innsbruck MitoCom<br />
|discipline=Mitochondrial Physiology<br />
}}<br />
{{Labeling<br />
|area=Respiration, Instruments;methods, mt-Biogenesis;mt-density, Comparative MiP;environmental MiP, Exercise physiology;nutrition;life style, mt-Medicine, mt-Awareness<br />
|organism=Human, Mouse<br />
|tissues=Heart, Skeletal muscle<br />
|model cell lines=Fibroblast<br />
|preparations=Permeabilized cells, Permeabilized tissue, Isolated Mitochondria<br />
|topics=Coupling efficiency;uncoupling, Flux control, Threshold;excess capacity<br />
|couplingstates=LEAK, ROUTINE, OXPHOS, ETS<br />
|substratestates=CI, CII, ETF, GpDH, CI+II<br />
|instruments=Oxygraph-2k, Protocol, Theory<br />
|discipline=Mitochondrial Physiology<br />
}}<br />
<br />
[[File:BlueBook openAccess.png|right|250px]]<br />
<br />
__TOC__<br />
<br />
<br />
= Bioblast online references and notes =<br />
* '''Preface''' <br />
: '''References Preface''' <br />
# [[Gnaiger 2007 MitoPathways|Gnaiger E ed (2007) Mitochondrial Pathways and Respiratory Control. 1st ed. OROBOROS MiPNet Publications, Innsbruck: 96 pp]]<br />
<br />
:>> [[MiPNet_Reference_Laboratories|WorldWide MiPNet]]<br />
:>> [http://www.oroboros.at/?O2k-Protocols O2k-Protocols]<br />
:>> [[O2k-Publications]]<br />
:>> [[MitoPedia Glossary: Terms and abbreviations]]<br />
<br />
<br />
== Chapter 1. OXPHOS analysis ==<br />
=== References OXPHOS analysis ===<br />
# [[Altmann 1894 Verlag Von Veit & Comp | Altmann R (1894) Die Elementarorganismen und ihre Beziehungen zu den Zellen. Zweite vermehrte Auflage. Verlag Von Veit & Comp, Leipzig. 160 pp, 34 Tafeln.]]<br />
# [[Dawson 2005 J Physiol | Dawson KD, Baker DJ, Greenhaff PL, Gibala MJ (2005) An accute decrease in TCA cycle intermediates does not affect aerobic energy delivery in contracting rat skeletal muscle. J Physiol 565: 637-643.]]<br />
# [[Garlid 1993 BTK | Garlid KD, Semrad C, Zinchenko V (1993) Does redox slip contribute significantly to mitochondrial respiration? In: Schuster S, Rigoulet M, Ouhabi R, Mazat J-P (eds) Modern Trends in Biothermokinetics. Plenum Press, New York, London: 287-293.]]<br />
# [[Gibala 1998 Am J Physiol Endocrinol Metab | Gibala 1998 Am J Physiol Endocrinol Metab|Gibala MJ, MacLean DA, Graham TE, Saltin B (1998) Tricarboxylic acid cycle intermediate pool size and estimated cycle flux in human muscle during exercise. Am J Physiol Endocrinol Metab 275: E235-E242.]] - concentrations of TCA cycle intermediates.<br />
# [[Gnaiger 1983 J Exp Zool|Gnaiger E (1983) Heat dissipation and energetic efficiency in animal anoxibiosis. Economy contra power. J Exp Zool 228: 471-490.]]<br />
# [[Gnaiger 1993 Hypoxia|Gnaiger E (1993) Efficiency and power strategies under hypoxia. Is low efficiency at high glycolytic ATP production a paradox? In: Surviving Hypoxia: Mechanisms of Control and Adaptation. Hochachka PW, Lutz PL, Sick T, Rosenthal M, Van den Thillart G (eds) CRC Press, Boca Raton, Ann Arbor, London, Tokyo: 77-109.]] - The Gibbs force of phorphorylation of ADP to ATP is ''F''<sub>ATP</sub> = 52 to 66 kJ/mol ATP under intracellular conditions.<br />
# [[Gnaiger 1993 Pure Appl Chem|Gnaiger E (1993) Nonequilibrium thermodynamics of energy transformations. Pure Appl Chem 65: 1983-2002.]]<br />
# [[Gnaiger 2003 Adv Exp Med Biol|Gnaiger E (2003) Oxygen conformance of cellular respiration. A perspective of mitochondrial physiology. Adv Exp Med Biol 543: 39-55.]]<br />
# [[Gnaiger 2002 Biochem Soc Trans|Gnaiger E, Kuznetsov AV (2002) Mitochondrial respiration at low levels of oxygen and cytochrome c. Biochem Soc Trans 30: 252-258.]]<br />
# [[Gnaiger 2000 Life in the Cold|Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000b) Mitochondria in the cold. In: Life in the Cold. (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York: pp 431-442.]] – MiR05 as the basis of MiR06.<br />
# [[Gnaiger 1998 Biochim Biophys Acta | Gnaiger E, Lassnig B, Kuznetsov AV, Margreiter R (1998) Mitochondrial respiration in the low oxygen environment of the cell: Effect of ADP on oxygen kinetics. Biochim Biophys Acta 1365: 249-254.]]<br />
# [[Gnaiger 1998 J Exp Biol | Gnaiger E, Lassnig B, Kuznetsov AV, Rieger G, Margreiter R (1998) Mitochondrial oxygen affinity, respiratory flux control, and excess capacity of cytochrome c oxidase. J Exp Biol 201: 1129-1139.]]<br />
# [[Gueguen 2005 J Physiol | Gueguen N, Lefaucheur L, Ecolan P, Fillaut M, Herpin P (2005) Ca2+-activated myosin-ATPases, creatine and adenylate kinases regulate mitochondrial function according to myofibre type in rabbit. J Physiol 564: 723-735.]]<br />
# [[Hatefi 1962 J Biol Chem | Hatefi Y, Haavik AG, Fowler LR, Griffiths DE (1962) Studies on the electron transfer system. XLII. Reconstitution of the electron transfer system. J Biol Chem 237: 2661-2669.]]<br />
# [[Kuznetsov 2004 Am J Physiol Heart Circ Physiol | Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome c release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286: H1633–H1641.]] – Cytochrome ‘’c’’ test.<br />
# [[Lemieux 2006 Biochim Biophys Acta | Lemieux H, Garedew A, Blier PU, Tardif J-C, Gnaiger E (2006) Temperature effects on the control and capacity of mitochondrial respiration in permeabilized fibers of the mouse heart. Biochim Biophys Acta, EBEC Short Reports Suppl 14: 201-202.]]<br />
# [[Mitchell 1961 Nature | Mitchell P (1961) Coupling of phosphorylation to electron and hydrogen transfer by a chemi-osmotic type of mechanism. Nature 191: 144-148.]]<br />
# Mitchell P (1966) Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Glynn Research Ltd, Bodmin: 192 pp. - ''The Grey Book'' 1.<br />
# Mitchell P (1968) Chemiosmotic coupling and energy transduction. Glynn Research Ltd, Bodmin: 111 pp. - ''The Grey Book'' 2.<br />
# [[Mitchell 1967 Biochem J | Mitchell P, Moyle J (1967) Respiration-driven proton translocation in rat liver mitochondria. Biochem J 105: 1147-1162.]]<br />
# [[Mootha 1997 Am J Physiol | Mootha VK, Arai AE, Balaban RS (1997) Maximum oxidative phosphorylation capacity of the mammalian heart. Am J Physiol 272: H769-H775.]] – [Pi] <10 mM and [ADP] <0.4 mM limit OXPHOS in isolated heart mitochondria.<br />
# [[Nicholson 2012 Nature | Nicholson JK, Holmes E, Kinross JM, Darzi AW, Takats Z, Lindon JC (2012) Metabolic phenotyping in clinical and surgical environments. Nature 491: 384-392.]]<br />
# [[Owen 2002 J Biol Chem | Owen OE, Kalhan SC, Hanson RW (2002) The key role of anaplerosis and cataplerosis for citric acid cycle function. J Biol Chem 277: 30409-30412.]]<br />
# [[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]] - >90% saturation is reached only >5 mM ADP, yet few studies use such high [ADP] in permeabilized tissues and cells. - Oxygen limitation of respiration below air saturation.<br />
# [[Puchowicz 2004 Mitochondrion | Puchowicz MA, Varnes ME, Cohen BH, Friedman NR, Kerr DS, Hoppel CL (2004) Oxidative phosphorylation analysis: assessing the integrated functional activity of human skeletal muscle mitochondria – case studies. Mitochondrion 4: 377-385.]] - Cytochrome ''c'' test.<br />
# [[Rasmussen 2000 Mol Cell Biochem | Rasmussen UF, Rasmussen HN (2000) Human quadriceps muscle mitochondria: A functional characterization. Mol Cell Biochem 208: 37-44.]] - Cytochrome ''c'' test.<br />
# [[Renner 2003 Biochim Biophys Acta | Renner K , Amberger A, Konwalinka G, Gnaiger E (2003) Changes of mitochondrial respiration, mitochondrial content and cell size after induction of apoptosis in leukemia cells. Biochim Biophys Acta 1642: 115-123.]]<br />
# [[Rossignol 2003 Biochem J | Rossignol R, Faustin B, Rocher C, Malgat M, Mazat JP, Letellier T (2003) Mitochondrial threshold effects. Biochem J 370: 751-762.]]<br />
# [[Saks 1998 Mol Cell Biochem | Saks VA, Veksler VI, Kuznetsov AV, Kay L, Sikk P, Tiivel T, Tranqui L, Olivares J, Winkler K, Wiedemann F, Kunz WS (1998) Permeabilised cell and skinned fiber techniques in studies of mitochondrial function in vivo. Mol Cell Biochem 184: 81-100.]] - The apparent ''K''<sub>m</sub> for ADP increases up to 0.5 mM in some permeabilized muscle fibres.<br />
# [[Territo 2000 Am J Physiol Cell Physiol| Territo PR, Mootha VK, French SA, Balaban RS (2000) Ca<sup>2+</sup> activation of heart mitochondrial oxidative phosphorylation: role of the F0/F1-ATPase. Am J Physiol Cell Physiol 278: C423-C435.]]<br />
<br />
== Chapter 2. Mitochondrial pathways to Complex I: Respiratory substrate control with pyruvate, malate and glutamate ==<br />
<br />
=== References CI ===<br />
# Brandt U (2006) Energy converting NADH:quinone oxidoreductase (Complex I). Annu Rev Biochem 75: 69-92.<br />
# Brewer GJ, Jones TT, Wallimann T, Schlattner U (2004) Higher respiratory rates and improved creatine stimulation in brain mitochondria isolated with antioxidants. Mitochondrion 4: 49-57.<br />
# Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. III. The steady state. J Biol Chem 217: 409-427. - Substrate depletion in isolated mitochondria is achieved in State 2: ADP is added to induce a transient stimulation of oxygen flux based on oxidation of endogenous substrates. <br />
# Digerness SB, Reddy WJ (1976) The malate-aspartate shuttle in heart mitochondria. J Mol Cell Cardiol. 8: 779-785.<br />
# Duchen MR (2004) Roles of mitochondria in health and disease. Diabetes 53, Suppl 1: S96-S102. - Mitochondrial glutamate dehydrogenase is particularly active in astrocytes, preventing glutamate induced neurotoxicity.<br />
# [[Gnaiger 2009 Int J Biochem Cell Biol|Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837–1845.]]<br />
# Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97: 11080-11085. - Equilibrium ratio of malate to fumarate is 4.1.<br />
# Hildyard JCW, Halestrap AP (2003) Identification of the mitochondrial pyruvate carrier in ''Saccharomyces cerevidiae''. Biochem J 374: 607-611.<br />
# Johnson G, Roussel D, Dumas JF, Douay O, Malthiery Y, Simard G, Ritz P (2006) Influence of intensity of food restriction on skeletal muscle mitochondrial energy metabolism in rats. Am J Physiol Endocrinol Metab 291: E460-E467. - Uncoupling stimulates coupled OXPHOS respiration, PM<sub>''P''</sub>, by 14%.<br />
# Kemp RB, Hoare S, Schmalfeldt M, Bridge CM, Evans PM, Gnaiger E (1994) A thermochemical study of the production of lactate by glutaminolysis and glycolysis in mouse macrophage hybridoma cells. In What is Controlling Life? (Gnaiger E, Gellerich FN, Wyss M, eds) Modern Trends in BioThermoKinetics 3, Innsbruck Univ Press: 226-231. - Glutamate derived from hydrolyzation of glutamine is a very important aerobic substrate in cultured cells.<br />
# Lemasters JJ (1984) The ATP-to-oxygen stoichiometries of oxidative phosphorylation by rat liver mitochondria. J Biol Chem 259: 13123-13130. - Malonate added to inhibit the succinate-fumarate reaction exerts only a minor effect on liver mitochondrial respiration.<br />
# Maechler P, Carobbio S, Rubi B (2006) In beta-cells, mitochondria integrate and generate metabolic signals controlling insulin secretion. Int J Biochem Cell Biol 38: 696-709.<br />
# Messer JI, Jackman MR, Willis WT (2004) Pyruvate and citric acid cycle carbon requirements in isolated skeletal muscle mitochondria. Am J Physiol Cell Physiol 286: C565-572. - With malate alone and saturating [ADP] isolated rat skeletal muscle mitochondria respire at only 1.3% of OXPHOS capacity with pyruvate+malate. Pyruvate alone yields only 2.1% of OXPHOS capacity (State P) with PM.<br />
# Nicholls DG, Ferguson SJ (2002) Bioenergetics 3, Academic Press, London. 287 pp. - Carriers.<br />
# Ouhabi R, Boue-Grabot M, Mazat J-P (1994) ATP synthesis in permeabilized cells: Assessment of the ATP/O ratios in situ. In What is Controlling Life? (Gnaiger E, Gellerich FN, Wyss M, eds) Modern Trends in BioThermoKinetics 3, Innsbruck Univ Press: 141-144. - In fibroblasts, GM<sub>''P''</sub> supports a higher respiratory flux than PM<sub>''P''</sub>.<br />
# O’Donnell JM, Kudej RK, LaNoue KF, Vatner SF, Lewandowski ED (2004) Limited transfer of cytosolic NADH into mitochondria at high cardiac workload. Am J Physiol Heart Circ Physiol 286: H2237-H2242.<br />
# Puchowicz MA, Varnes ME, Cohen BH, Friedman NR, Kerr DS, Hoppel CL (2004) Oxidative phosphorylation analysis: assessing the integrated functional activity of human skeletal muscle mitochondria – case studies. Mitochondrion 4: 377-385. - OXPHOS with glutamate alone is 50% to 85% of respiration with glutamate+malate. Accumulation of fumarate inhibits succinate dehydrogenase and glutamate dehydrogenase (Caughey et al 1957; Dervartanian, Veeger 1964). - OXPHOS with glutamate+malate is identical or 10% higher than with pyruvate+malate.<br />
# Rasmussen UF, Rasmussen HN (2000) Human quadriceps muscle mitochondria: A functional characterization. Mol Cell Biochem 208: 37-44. - Uncoupling stimulates coupled OXPHOS respiration, PMP, by 15% in human skeletal muscle. OXPHOS with glutamate alone is 50% to 85% of respiration with glutamate+malate. - OXPHOS with glutamate+malate is identical or 10% higher than with pyruvate+malate.<br />
# Thomas et al (2004) - OXPHOS in human skeletal muscle for PM<sub>''P''</sub> is 25% higher than for GM<sub>''P''</sub>.<br />
# Winkler-Stuck K, Kirches E, Mawrin C, Dietzmann K, Lins H, Wallesch CW, Kunz WS, Wiedemann FR (2005) Re-evaluation of the dysfunction of mitochondrial respiratory chain in skeletal muscle of patients with Parkinson's disease. J Neural Transm 112: 499-518. - OXPHOS in human skeletal muscle for PM<sub>''P''</sub> is 16% higher than for GM<sub>''P''</sub>.<br />
<br />
=== Notes CI ===<br />
# Schwerzmann et al (1989) Proc Natl Acad Sci U S A 86: 1583-1587. “Of the substrates used here, pyruvate/malate activates the chain at complex I, glutamate/malate and succinate at complexes II and III, ..” - This consideration of glutamate+malate requires correction.<br />
# Ponsot et al (2005) J Cell Physiol 203: 479-486. (a) Respiration (State 3) in permeabilized fibres with malate alone gave 25-50% of the flux with pyruvate+malate. This most likely indicates a large content of endogenous mitochondrial substrates, which interfere to an unknown degree with the kinetics of respiration after addition of exogenous substrates. In such a study, the conventional initial depletion of endogenous substrates would be most important. (b) Maximal respiration rates in muscle should be evaluated at saturating or high Pi, since at a Pi concentration of 3 mM OXPHOS respiration is phosphate limited.<br />
# Hulbert et al (2006) J Comp Physiol B 176: 93-105. Addition of ‘sparking malate concentrations’. This term can probably be derived from the misconception that tricarboxylic acid cycle intermediates are conserved during respiration of isolated mitochondria. 380 µM malate (instead of mM concentrations) in conjunction with 2.4 mM pyruvate were used, which makes a comparison difficult between different tissues and different species: the low malate concentration may limit PMP flux at various degrees in the different sources of mitochondria, and GMP may support higher fluxes than PMP at tissue- and species-specific degrees.<br />
<br />
<br />
== Chapter 3. Mitochondrial pathways to Complex II. Glycerophosphate dehydrogenase and electrontransferring flavoprotein ==<br />
<br />
=== References CII ===<br />
# Capel F, Rimbert V, Lioger D, Diot A, Rousset P, Patureau Mirand P, Boirie Y, Morio B, Mosoni L (2005) Due to reverse electron transfer, mitochondrial H2O2 release increases with age in human vastus lateralis muscle although oxidative capacity is preserved. Mech Ageing Develop 126: 505-511. - With succinate alone OXPHOS is 30-40% lower than with succinate+rotenone in human skeletal muscle mitochondria.<br />
# Cecchini G (2003) Function and structure of Complex II of the respiratory chain. Annu Rev Biochem 72: 77-109.<br />
# Ernster L, Nordenbrand K (1967) Skeletal muscle mitochondria. In: Estabrook RW, Pullman ME (eds) Meth Enzymol: 86-94. – With succinate alone OXPHOS is 30-40% lower than with succinate+rotenone in rat skeletal muscle mitochondria.<br />
# Jackman MR, Willis WT (1996) Characteristics of mitochondria isolated from type I and type IIb skeletal muscle. Am J Physiol Cell Physiol 270: C673-678. - Glycerophosphate oxidation is 10-fold higher in rabbit gracilis mitochondria compared to soleus.<br />
# Lehninger AL (1970) Biochemistry. The molecular basis of cell structure and function Worth. 833 pp. - Oxaloacetate is a more potent competitive inhibitor of succinate dehydrogenase than malonate even at small concentration (p. 352).<br />
# Muller FL, Liu Y, Abdul-Ghani MA, Lustgarten MS, Bhattacharya A, Jang YC, Van Remmen H (2008) High rates of superoxide production in skeletal-muscle mitochondria respiring on both Complex I- and Complex II-linked substrates. Biochem J 409: 491–499. - Addition of malate inhibits superoxide production with succinate, probably due to the oxaloacetate inhibition of CII.<br />
# Rauchova H, Drahota Z, Rauch P, Fato R, Lenaz G (2003) Coenzyme Q releases the inhibitory effect of free fatty acids on mitochondrial glycerophosphate dehydrogenase. Acta Biochim Polonica 50: 405-413. - Glycerophosphate is an important substrate for respiration in brown adipose tissue mitochondria.<br />
# Rasmussen UF, Rasmussen HN (2000) Human quadriceps muscle mitochondria: A functional characterization. Mol Cell Biochem 208: 37-44. – Glycerophosphate oxidation is relatively slow.<br />
# Sun F, Huo X, Zhai Y, Wang A, Xu J, Su D, Bartlam M, Rao Z (2005) Crystal structure of mitochondrial respiratory membrane protein Complex II. Cell 121: 1043–1057.<br />
<br />
=== Notes CII === <br />
# Ponsot et al (2005) J Cell Physiol 203: 479-486. ‘.. the mitochondrial form of GPDH, which produces FADH2 within the mitochondrial matrix and provides electrons to Compoex II of the phosphorylation chain’. – The mitochondrial glycerophosphate dehydrogenase (GpDH), located on the outer side of the inner mitochondrial membrane, does not provide electrons to CII, but feeds electrons into the Q-cycle entirely independent of CII. FADH2 is not produced within the mitochondrial matrix. Electron transfer takes place from the mitochondrial inner membrane flavoprotein-linked glycerophosphate dehydrogenase to CoQ.<br />
# In the first edition of ‘Mitochondrial Pathways and Respiratory Control’ (2007), the term ‘electron transport’ is used synonymously for ‘electron transfer’.<br />
<br />
<br />
<br />
== Chapter 4. Mitochondrial pathways to Complexes I+II: Convergent electron transfer at the Q-Junction and additive effect of substrate combinations ==<br />
<br />
=== References CI+II ===<br />
# Aragonés J, Schneider M, Van Geyte K, Fraisl P, Dresselaers T, Mazzone M, Dirkx R, Zacchigna S, Lemieux H, Jeoung NH, Lambrechts D, Bishop T, Lafuste P, Diez-Juan A, K Harten S, Van Noten P, De Bock K, Willam C, Tjwa M, Grosfeld A, Navet R, Moons L, Vandendriessche T, Deroose C, Wijeyekoon B, Nuyts J, Jordan B, Silasi-Mansat R, Lupu F, Dewerchin M, Pugh C, Salmon P, Mortelmans L, Gallez B, Gorus F, Buyse J, Sluse F, Harris RA, Gnaiger E, Hespel P, Van Hecke P, Schuit F, Van Veldhoven P, Ratcliffe P, Baes M, Maxwell P, Carmeliet P (2008) Deficiency or inhibition of oxygen sensor Phd1 induces hypoxia tolerance by reprogramming basal metabolism. Nat Genet 40: 170-180. - OXPHOS analysis for phenotyping.<br />
# Bianchi C, Genova ML, Parenti Castelli G, Lenaz G (2004) The mitochondrial respiratory chain is partially organized in a supercomplex assembly: kinetic evidence using flux control analysis. J Biol Chem 279: 36562-36569.<br />
# Boushel R, Gnaiger E, Schjerling P, Skovbro M, Kraunsoe R, Flemming D (2007) Patients with Type 2 Diabetes have normal mitochondrial function in skeletal muscle. Diabetologia 50: 790-796.<br />
# Boushel R, Gnaiger E, Calbet JA, Gonzalez-Alonso J, Wright-Paradis C, Sondergaard H, Ara I, Helge JW, Saltin B (2011) Muscle mitochondrial capacity exceeds maximal oxygen delivery in humans. Mitochondrion 11: 303-307.<br />
# Capel F, Rimbert V, Lioger D, Diot A, Rousset P, Patureau Mirand P, Boirie Y, Morio B, Mosoni L (2005) Due to reverse electron transfer, mitochondrial H2O2 release increases with age in human vastus lateralis muscle although oxidative capacity is preserved. Mech Ageing Develop 126: 505-511. - CI+II substrate combination.<br />
# Chance B (1965) Reaction of oxygen with the respiratory chain in cells and tissues. J Gen Physiol 49: 163-188. - Glutamate+succinate as respiratory substrate combination, without comparison of flux with different substrates.<br />
# Costa LE, Boveris A, Koch OR, Taquini AC (1988) Liver and heart mitochondria in rats submitted to chronic hypobaric hypoxia. Am J Physiol Cell Physiol 255: C123-C129.<br />
# Digerness SB, Reddy WJ (1976) The malate-aspartate shuttle in heart mitochondria. J Mol Cell Cardiol. 8: 779-785.<br />
# Eberhart K, Rainer J, Bindreither D, Ritter I, Gnaiger E, Kofler R, Oefner PJ, Renner K (2011) Glucocorticoid-induced alterations in mitochondrial membrane properties and respiration in childhood acute lymphoblastic leukemia. Biochim Biophys Acta 1807: 719-725.<br />
# Estabrook R (1967) Mitochondrial respiratory control and the polarographic measurement of ADP:O ratios. Meth Enzymol 10: 41-47.<br />
# Garait B, Couturier K, Servais S, Letexier D, Perrin D, Batandier C, Rouanet J-L, Sibille B, Rey B, Leverve X, Favier R (2005) Fat intake reverses the beneficial effects of low caloric intake on skeletal muscle mitochondrial H2O2 production. Free Radic Biol Med 39: 1249–1261. - GM<sub>''P''</sub>/GMS<sub>''P''</sub> substrate control ratio in skeletal muscle of rats fed on various diets ranges from 0.7 to 0.8. <br />
# Garedew A, Lemieux H, Schachner T, Blier PU, Tardif J-C, Gnaiger E (2006) High excess capacity of cytochrome c oxidase in permeabilized fibers of the mouse heart. Biochim Biophys Acta, EBEC Short Reports Suppl 14: 167-168.<br />
# [[Gnaiger 2009 Int J Biochem Cell Biol|Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837–1845.]]<br />
# Gnaiger E, Wright-Paradis C, Sondergaard H, Lundby C, Calbet JA, Saltin B, Helge J, Boushel R (2005) High-resolution respirometry in small biopsies of human muscle: correlations with body mass index and age. Mitochondr Physiol Network 10.9: 14-15. http://www.mitophysiology.org/?gnaigere.<br />
# González-Flecha B, Cutrin JC, Boveris A (1993) Time course and mechanism of oxidative stress and tissue damage in rat liver subjected to in vivo ischemia-reperfusion. J Clin Invest 91: 456-464. - Respiration was measured in states GS<sub>''P''</sub> and GM<sub>''P''</sub>.<br />
# Gutman M, Coles CJ, Singer TP, Casida JE (1971) On the functional organization of the respiratory chain at the dehydrogenase-coenzyme Q junction. Biochemistry 10: 2036-2043.<br />
# Hansford RG, Hogue BA, Mildaziene V (1997) Dependence of H2O2 formation by rat heart mitochondria on substrate availability and donor age. J Bioenerg Biomembr 29: 89–95.<br />
# Hatefi Y, Haavik AG, Fowler LR, Griffiths DE (1962) Studies on the electron transfer system. XLII. Reconstitution of the electron transfer system. J Biol Chem 237: 2661-2669.<br />
# König T, Nicholls DG, Garland PB (1969) The inhibition of pyruvate and Ls(+)-isocitrate oxidation by succinate oxidation in rat liver mitochondria. Biochem J 114: 589-596.<br />
# Krebs HA (1935) CXCVII. Metabolism of amino-acids. III. Deamination of amino-acids. Biochem J 29: 1620-1644.<br />
# Kunz WS, Kudin A, Vielhaber S, Elger CE, Attardi G, Villani G (2000) Flux control of cytochrome c oxidase in human skeletal muscle. J Biol Chem 275: 27741-27745.<br />
# Kuznetsov AV, Clark JF, Winkler K, Kunz WS (1996) Increase of flux control of cytochrome c oxidase in copper-deficient mottled brindled mice. J Biol Chem 271: 283-288.<br />
# Kuznetsov AV, Strobl D, Ruttmann E, Königsrainer A, Margreiter R, Gnaiger E (2002) Evaluation of mitochondrial respiratory function in small biopsies of liver. Analyt Biochem 305: 186-194. - S(Rot) alone supports a higher flux than GM in liver mitocondria.<br />
# Kuznetsov AV, Winkler K, Kirches E, Lins H, Feistner H, Kunz WS (1997) Application of inhibitor titrations for the detection of oxidative phosphorylation defects in saponin-skinned muscle fibers of patients with mitochondrial diseases. Biochim Biophys Acta 1360: 142-150. - OXPHOS with glutamate+malate is identical or 10% higher than with pyruvate+malate.<br />
# LaNoue KF, Bryla J, Williamson JR (1972) Feedback interactions in the control of citric acid cycle activity in rat heart mitochondria. J Biol Chem 247: 667-679.<br />
# LaNoue KF, Schoolwerth AC (1979) Metabolite transport in mitochondria. Annu Rev Biochem 48: 871-922.<br />
# Lehninger AL (1970) Biochemistry. The molecular basis of cell structure and function Worth. 833 pp. - Electron transport chain.<br />
# Lemieux H, Garedew A, Blier PU, Tardif J-C, Gnaiger E (2006) Temperature effects on the control and capacity of mitochondrial respiration in permeabilized fibers of the mouse heart. Biochim Biophys Acta, EBEC Short Reports Suppl 14: 201-202.<br />
# [[Lemieux 2011 Int J Biochem Cell Biol|Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43: 1729–1738.]] - SUIT protocols.<br />
# Lenaz G, Genova ML (2009) Structural and functional organization of the mitochondrial respiratory chain: A dynamic super-assembly. Int J Biochem Cell Biol 41: 1750-1772.<br />
# Llesuy S, Evelson P, González-Flecha B, Peralta J, Carreras MC, Poderoso JJ, Boveris A (1994) Oxidative stress in muscle and liver of rats with septic syndrome. Free Radic Biol Med 16: 445-451. - GM<sub>''P''</sub>/GS<sub>''P''</sub> substrate control ratios are 0.8 and 0.7 in liver mitochondria (male Wistar and female Sprague-Dawley, respectively).<br />
# Mogensen M, Sahlin K (2005) Mitochondrial efficiency in rat skeletal muscle: influence of respiration rate, substrate and muscle type. Acta Physiol Scand 185: 229-236. - The flux control ratio of palmitoylcarnitine+malate/PM (OXPHOS) is 0.6 for mitochondria isolated from rat extensor digitorum longus muscle (mainly type II fibre type), but is 0.95 in rat soleus muscle (type I fibre type).<br />
# Muller FL, Liu Y, Abdul-Ghani MA, Lustgarten MS, Bhattacharya A, Jang YC, Van Remmen H (2008) High rates of superoxide production in skeletal-muscle mitochondria respiring on both Complex I- and Complex II-linked substrates. Biochem J 409: 491–499.<br />
# Nicholls DG, Ferguson SJ (2002) Bioenergetics 3, Academic Press, London. 287 pp. - Electron transport chain.<br />
# [[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]] - SUIT protocols.<br />
# [[Pesta 2011 Am J Physiol Regul Integr Comp Physiol|Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.]] - SUIT protocols.<br />
# Rasmussen HN, Rasmussen UF (1997) Small scale preparation of skeletal muscle mitochondria, criteria for integrity, and assays with reference to tissue function. Mol Cell Biochem 174: 55-60.<br />
# Rasmussen UF, Rasmussen HN (2000) Human quadriceps muscle mitochondria: A functional characterization. Mol Cell Biochem 208: 37-44. - OXPHOS with malate+palmitoylcarnitine is 0.60 of OXPHOS with GM.<br />
# Rasmussen UF, Rasmussen HN, Krustrup P, Quistorff B, Saltin B, Bangsbo J (2001) Aerobic metabolism of human quadriceps muscle: in vivo data parallel measurements on isolated mitochondria. Am J Physiol Endocrinol Metab 280: E301–E307.<br />
# Rossignol R, Faustin B, Rocher C, Malgat M, Mazat JP, Letellier T (2003) Mitochondrial threshold effects. Biochem J 370: 751-762.<br />
# Rumsey WL, Schlosser C, Nuutinen EM, Robiolio M, Wilson DF (1990) Cellular energetics and the oxygen dependence of respiration in cardiac myocytes isolated from adult rat. J Biol Chem 265. 15392-15399. - 2.5-fold increase of respiration in the LEAK state with GMS compared to GM.<br />
# Schägger H, Pfeiffer K (2001) The ratio of oxidative phosphorylation complexes I-V in bovine heart mitochondria and the composition of respiratory chain supercomplexes. J Biol Chem 276: 37861-37867.<br />
# Short KR, Bigelow ML, Kahl J, Singh R, Coenen-Schimke J, Raghavakaimal S, Nair KS (2005) Decline in skeletal muscle mitochondrial function with aging in humans. Proc Natl Acad Sci U S A 102: 5618 - 5623.<br />
# Simon J, van Spanning RJM, Richardson DJ (2008) The organisation of proton motive and non-proton motive redox loops in prokaryotic respiratory systems. Biochim Biophys Acta 1777: 1480–1490.<br />
# Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271: C2053-C2061.<br />
# Steinlechner-Maran R, Eberl T, Kunc M, Schröcksnadel H, Margreiter R, Gnaiger E (1997) Respiratory defect as an early event in preservation/reoxygenation injury in endothelial cells. Transplantation 63: 136-142.<br />
# Stadlmann S, Rieger G, Amberger A, Kuznetsov AV, Margreiter R, Gnaiger E (2002) H2O2-mediated oxidative stress versus cold ischemia-reperfusion: mitochondrial respiratory defects in cultured human endothelial cells. Transplantation 74: 1800-1803.<br />
# Sugano T, Oshino N, Chance B (1974). Mitochondrial functions under hypoxic conditions. The steady states of cytochrome ''c'' reduction and energy metabolism. Biochim Biophys Acta 347: 340-358. - Glutamate+succinate as respiratory substrate combination, without comparison of flux with different substrates.<br />
# Tonkonogi M, Walsh B, Tiivel T, Saks V, Sahlin K (1999) Mitochondrial funciton in human skeletal muscle is not impaired by high intensity exercise. Eur J Physiol 437: 562-568.<br />
# Torres NV, Mateo F, Sicilia J, Meléndez-Hevia E. (1988) Distribution of the flux control in convergent metabolic pathways: theory and application to experimental and simulated systems. Int J Biochem 20: 161-165.<br />
# Villani G, Attardi G (1997) In vivo control of respiration by cytochrome c oxidase in wild-type and mitochondrial DNA mutation-carrying human cells. Proc Natl Acad Sci U S A 94: 1166-1171.<br />
# Villani G, Greco M, Papa S, Attardi G (1998) Low reserve capacity of cytochrome c oxidase capacity in vivo in the respiratory chain of a variety of human cell types. J Biol Chem 273: 31829-31836.<br />
# [[Votion 2012 PLoS One|Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890.]] - SUIT protocols.<br />
# Wiedemann FR, Winkler K, Kuznetsov AV, Bartels C, Vielhaber S, Feistner H, Kunz WS (1998) Impairment of mitochondrial function in skeletal muscle of patients with amyotrophic lateral sclerosis. J Neurol Sci 156: 65-72. - - OXPHOS with glutamate+malate is identical or 10% higher than with pyruvate+malate.<br />
# Wilson DF, Rumsey WL, Green TJ, Vanderkooi J (1988). The oxygen dependence of mitochondrial oxidative phosphorylation measured by a new optical method for measuring oxygen concentration. J Biol Chem 263: 2712-2718. - Glutamate+succinate as respiratory substrate combination, without comparison of flux with different substrates.<br />
# Zoccarato F, Cavallini L, Bortolami S, Alexandre A (2007) Succinate modulation of H2O2 release at NADH:ubiquinone oxidoreductase (complex I) in brain mitochondria. Biochem J 406: 125–129. - CI+II substrate combination.<br />
<br />
:>> [[O2k-Publications:_Q-Junction_Effect]]<br />
<br />
=== Notes CI+II ===<br />
# Identical GM<sub>''P''</sub>/GS<sub>''P''</sub> or GM<sub>''P''</sub>/GMS<sub>''P''</sub> ratios of 0.7 are reported for isolated mitochondria (Rasmussen and Rasmussen 2000; Capel et al 2005) and permeabilized fibres (Kunz et al 2000). For a review see Gnaiger (2009).<br />
* '''Correction''': Occasionally 'electron gating' was erroneously spelled as 'gaiting': p. 32; legends to Fig. 1.5 (p. 15) and 4.2 (p. 39).<br />
<br />
== Chapter 5. Respiratory states, coupling control and coupling control ratios ==<br />
<br />
=== References Respiratory states ===<br />
# Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. I. Kinetics of oxygen utilization. J Biol Chem 217: 383-393.<br />
# Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. III. The steady state. J Biol Chem 217: 409-427.<br />
# Chance B, Williams GR (1956) The respiratory chain and oxidative phosphorylation. Adv Enzymol17: 65-134.<br />
# Estabrook R (1967) Mitochondrial respiratory control and the polarographic measurement of ADP:O ratios. Meth Enzymol 10: 41-47.<br />
# [[Gnaiger_2001_Respir Physiol|Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128: 277-297.]]<br />
# [[Gnaiger_1998_Biochim Biophys Acta|Gnaiger E, Lassnig B, Kuznetsov AV, Margreiter R (1998) Mitochondrial respiration in the low oxygen environment of the cell: Effect of ADP on oxygen kinetics. Biochim Biophys Acta 1365: 249-254.]] - Oxygen kinetics is different in the LEAK state without adenylates (''L''<sub>N</sub>) and State 4 (LEAK state with ATP, ''L''<sub>N</sub>).<br />
# [[Gnaiger 2000 Proc Natl Acad Sci U S A|Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97: 11080-11085.]]<br />
# König T, Nicholls DG, Garland PB (1969) The inhibition of pyruvate and Ls(+)-isocitrate oxidation by succinate oxidation in rat liver mitochondria. Biochem J 114: 589-596. - 3½ has been suggested to indicate an intermediate mitochondrial energy state somewhere between States 3 and 4. Would, therefore, State 4 be considered as being somewhere between State 3 and 5?<br />
# Nicholls DG, Ferguson SJ (2002) Bioenergetics 3, Academic Press, London. 287 pp. - ''Misconception on State 2.''<br />
<br />
:>> [[MitoPedia Glossary: Respiratory states]]<br />
<br />
== Chapter 6. Conversions of metabolic fluxes ==<br />
<br />
=== References Conversions ===<br />
<br />
<br />
# Brooks GA, Hittelman KJ, Faulkner JA, Beyer RE (1971) Temperature, skeletal muscle mitochondrial functions, and oxygen debt. Am J Physiol 220: 1053-1059.<br />
# Gnaiger E (1983) Symbols and units: Toward standardization. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York: 352-358.<br />
# Slater EC, Rosing J, Mol A (1973) The phosphorylation potential generated by respiring mitochondria. Biochim Biophys Acta 292: 534-553.<br />
<br />
<br />
<br />
== Apendix: Abbreviations ==<br />
<br />
=== A1. Respiratory coupling states and coupling control ratios ===<br />
<br />
=== A2. Substrates, uncouplers and inhibitors ===<br />
<br />
:>> [[List of substrates and metabolites]]<br />
:>> [[List of uncouplers]]<br />
:>> [[List of inhibitors]]<br />
<br />
<br />
== Follow-up ==<br />
<br />
* The 2007 Edition is cited in: <br />
:# [[Muller 2008 Biochem J|Muller FL et al (2008) High rates of superoxide production in skeletal-muscle mitochondria respiring on both Complex I- and Complex II-linked substrates. Biochem J 409: 491–499.]]<br />
:# [[Droese_2009_Biochim_Biophys_Acta|Dröse S, Hanley PJ, Brandt U (2009) Ambivalent effects of diazoxide on mitochondrial ROS production at respiratory chain complexes I and III. Biochim Biophys Acta 1790: 558-565.]]<br />
:# [[Hickey_2009_Am_J_Physiol_Cell_Physiol|Hickey AJ, Chai CC, Choong SY, de Freitas Costa S, Skea GL, Phillips AR, Cooper GJ (2009) Impaired ATP turnover and ADP supply depress cardiac mitochondrial respiration and elevate superoxide in nonfailing spontaneously hypertensive rat hearts. Am J Physiol Cell Physiol 297: C766–C774.]]<br />
:# Hoffman DL, Brookes PS (2009) Oxygen sensitivity of mitochondrial reactive oxygen species generation depends on metabolic conditions. J Biol Chem 284: 16236-16245.<br />
:# Sahlin K (2009) Control of lipid oxidation at the mitochondrial level. Appl Physiol Nutr Metab 34: 382-388:<br />
:# Bazil JN, Buzzard GT, Rundell AE (2010) Modeling mitochondrial bioenergetics with integrated volume dynamics. PLoS Comput Biol. 6: e1000632.<br />
:# Sivitz WI, Yorek MA (2010) Mitochondrial dysfunction in diabetes: from molecular mechanisms to functional significance and therapeutic opportunities. Antioxid Redox Signal 12: 537-577.<br />
:# Sivitz WI (2010) Mitochondrial Dysfunction in Obesity and Diabetes. US Endocrinology 6: 20-27.<br />
:# [[Iftikar 2010 JEMBE|Iftikar F, MacDonald JR, Hickey AJ (2010) Thermal limits of portunid crab heart mitochondria: Could more thermo-stable mitochondria advantage invasive species? JEMBE 395: 232-239.]]<br />
:# [[Mittal 2011 HPB (Oxford)|Mittal A, Hickey AJ, Chai CC, Loveday BP, Thompson N, Dare A, Delahunt B, Cooper GJ, Windsor JA, Phillips AR (2011) Early organ-specific mitochondrial dysfunction of jejunum and lung found in rats with experimental acute pancreatitis. HPB (Oxford) 13: 332-341.]]<br />
:# [[Pichaud 2011 Am J Physiol Regul Integr Comp Physiol|Pichaud N, Ballard JW, Tanguay RM, Blier PU (2011) Thermal sensitivity of mitochondrial functions in permeabilized muscle fibers from two populations of Drosophila simulans with divergent mitotypes. Am J Physiol Regul Integr Comp Physiol 301: R48-R59.]]<br />
:# [[Ye 2011 J Biol Chem|Ye F, Lemieux H, Hoppel CL, Hanson RW, Hakimi P, Croniger CM, Puchowicz M, Anderson VE, Fujioka H, Stavnezer E (2011) Peroxisome proliferator-activated receptor γ (PPARγ) mediates a Ski-induced shift from glycolysis to oxidative energy metabolism. J Biol Chem 286: 40013-40024.]]<br />
:# Cordeiro OD, Silva TS, Alves RN, Costas B, Wulff T, Richard N, de Vareilles M, Conceição LE, Rodrigues PM (2012) Changes in liver proteome expression of Senegalese sole (Solea senegalensis) in response to repeated handling stress. Mar Biotechnol (NY)14: 714-729.<br />
:# Everett MV, Antal CE, Crawford DL (2012) The effect of short-term hypoxic exposure on metabolic gene expression. J Exp Zool A Ecol Genet Physiol 317: 9-23.<br />
:# [[Miquel 2012 PLoS One|Miquel E, Cassina A, Martinez-Palma L, Bolatto C, Trias E, Gandelman M, Radi R, Barbeito L, Cassina P (2012) Modulation of astrocytic mitochondrial function by dichloroacetate improves survival and motor performance in inherited amyotrophic lateral sclerosis. PLoS One 7: e34776.]]<br />
:# [[De Paula Martins 2013 J Bioenerg Biomembr|de Paula Martins R, Glaser V, da Luz Scheffer D, de Paula Ferreira PM, Wannmacher CM, Farina M, de Oliveira PA, Prediger RD, Latini A (2013) Platelet oxygen consumption as a peripheral blood marker of brain energetics in a mouse model of severe neurotoxicity. J Bioenerg Biomembr 45: 449-457.]]<br />
:# [[Perry 2013 Diabetes|Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62: 1041-1053.]]<br />
:# Chu MJJ, Dare AJ, Bartlett ASJR, Phillips ARJ, Hickey AJ (2013) Effect of Hepatic Steatosis on Bioenergetic Function During Hepatic Ischemia-reperfusion: A Systematic Review. The Open Transplantation J 6: 10-32.<br />
:# [[Gispert_2013_Hum_Mol_Genet|Gispert S, Parganlija D, Klinkenberg M, Dröse S, Wittig I, Mittelbronn M, Grzmil P, Koob S, Hamann A, Walter M, Büchel F, Adler T, Hrabé de Angelis M, Busch DH, Zell A, Reichert AS, Brandt U, Osiewacz HD, Jendrach M, Auburger G (2013) Loss of mitochondrial peptidase Clpp leads to infertility, hearing loss plus growth retardation via accumulation of CLPX, mtDNA and inflammatory factors. Hum Mol Genet 22: 4871-4887.]]<br />
:# [[Hirzel 2013 J Recept Signal Transduct Res|Hirzel E, Lindinger PW, Maseneni S, Giese M, Rhein VV, Eckert A, Hoch M, Krähenbühl S, Eberle AN (2013) Differential modulation of ROS signals and other mitochondrial parameters by the antioxidants MitoQ, resveratrol and curcumin in human adipocytes. J Recept Signal Transduct Res 33:304-312.]]<br />
:# [[Cordero-Reyes_2014_J_Mol_Cell_Cardiol|Cordero-Reyes AM, Gupte AA, Youker KA, Loebe M, Hsueh WA, Torre-Amione G, Taegtmeyer H, Hamilton DJ (2014) Freshly isolated mitochondria from failing human hearts exhibit preserved respiratory function. J Mol Cell Cardiol doi: 10.1016/j.yjmcc.2013.12.029.]]<br />
<br />
* The 2010 Edition is cited in: <br />
:# Granados-Castro LF, Rodríguez-Rangel DS, Montaño M, Ramos C, Pedraza-Chaverri J (2013) Wood smoke exposure induces a decrease in respiration parameters and in the activity of respiratory complexes I and IV in lung mitochondria from guinea pigs. Environ Toxicol. doi: 10.1002/tox.21922.<br />
:# Maldonado F, Packard TT, Gómez M (2012) Understanding tetrazolium reduction and the importance of substrates in measuring respiratory electron transport activity. J Exp Marine Biol Ecol 434–435: 110–118.</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=Gnaiger_2020_BEC_MitoPathways&diff=58095Gnaiger 2020 BEC MitoPathways2014-03-19T14:55:13Z<p>Bauer Christiane: </p>
<hr />
<div>{{Publication<br />
|title=Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp. ISBN 978-3-9502399-6-6<br />
|info=[[Media:Gnaiger 2012 Mitochondr Physiol Network MitoPathways.pdf| '''Bioblast pdf''']]; [http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger_2012_MitoPathways]<br />
|authors=Gnaiger E<br />
|year=2012<br />
|journal=Mitochondr Physiol Network<br />
|keywords=Q-junction, Respiratory states, Flux control ratios<br />
|mipnetlab=AT_Innsbruck_Gnaiger E, AT Innsbruck OROBOROS, AT Innsbruck MitoCom<br />
|discipline=Mitochondrial Physiology<br />
}}<br />
{{Labeling<br />
|area=Respiration, Instruments;methods, mt-Biogenesis;mt-density, Comparative MiP;environmental MiP, Exercise physiology;nutrition;life style, mt-Medicine, mt-Awareness<br />
|organism=Human, Mouse<br />
|tissues=Heart, Skeletal muscle<br />
|model cell lines=Fibroblast<br />
|preparations=Permeabilized cells, Permeabilized tissue, Isolated Mitochondria<br />
|topics=Coupling efficiency;uncoupling, Flux control, Threshold;excess capacity<br />
|couplingstates=LEAK, ROUTINE, OXPHOS, ETS<br />
|substratestates=CI, CII, ETF, GpDH, CI+II<br />
|instruments=Oxygraph-2k, Protocol, Theory<br />
|discipline=Mitochondrial Physiology<br />
}}<br />
<br />
[[File:BlueBook openAccess.png|right|200px]]<br />
<br />
__TOC__<br />
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<br />
= Bioblast online references and notes =<br />
* '''Preface''' <br />
: '''References Preface''' <br />
# [[Gnaiger 2007 MitoPathways|Gnaiger E ed (2007) Mitochondrial Pathways and Respiratory Control. 1st ed. OROBOROS MiPNet Publications, Innsbruck: 96 pp]]<br />
<br />
:>> [[MiPNet_Reference_Laboratories|WorldWide MiPNet]]<br />
:>> [http://www.oroboros.at/?O2k-Protocols O2k-Protocols]<br />
:>> [[O2k-Publications]]<br />
:>> [[MitoPedia Glossary: Terms and abbreviations]]<br />
<br />
<br />
== Chapter 1. OXPHOS analysis ==<br />
=== References OXPHOS analysis ===<br />
# [[Altmann 1894 Verlag Von Veit & Comp | Altmann R (1894) Die Elementarorganismen und ihre Beziehungen zu den Zellen. Zweite vermehrte Auflage. Verlag Von Veit & Comp, Leipzig. 160 pp, 34 Tafeln.]]<br />
# [[Dawson 2005 J Physiol | Dawson KD, Baker DJ, Greenhaff PL, Gibala MJ (2005) An accute decrease in TCA cycle intermediates does not affect aerobic energy delivery in contracting rat skeletal muscle. J Physiol 565: 637-643.]]<br />
# [[Garlid 1993 BTK | Garlid KD, Semrad C, Zinchenko V (1993) Does redox slip contribute significantly to mitochondrial respiration? In: Schuster S, Rigoulet M, Ouhabi R, Mazat J-P (eds) Modern Trends in Biothermokinetics. Plenum Press, New York, London: 287-293.]]<br />
# [[Gibala 1998 Am J Physiol Endocrinol Metab | Gibala 1998 Am J Physiol Endocrinol Metab|Gibala MJ, MacLean DA, Graham TE, Saltin B (1998) Tricarboxylic acid cycle intermediate pool size and estimated cycle flux in human muscle during exercise. Am J Physiol Endocrinol Metab 275: E235-E242.]] - concentrations of TCA cycle intermediates.<br />
# [[Gnaiger 1983 J Exp Zool|Gnaiger E (1983) Heat dissipation and energetic efficiency in animal anoxibiosis. Economy contra power. J Exp Zool 228: 471-490.]]<br />
# [[Gnaiger 1993 Hypoxia|Gnaiger E (1993) Efficiency and power strategies under hypoxia. Is low efficiency at high glycolytic ATP production a paradox? In: Surviving Hypoxia: Mechanisms of Control and Adaptation. Hochachka PW, Lutz PL, Sick T, Rosenthal M, Van den Thillart G (eds) CRC Press, Boca Raton, Ann Arbor, London, Tokyo: 77-109.]] - The Gibbs force of phorphorylation of ADP to ATP is ''F''<sub>ATP</sub> = 52 to 66 kJ/mol ATP under intracellular conditions.<br />
# [[Gnaiger 1993 Pure Appl Chem|Gnaiger E (1993) Nonequilibrium thermodynamics of energy transformations. Pure Appl Chem 65: 1983-2002.]]<br />
# [[Gnaiger 2003 Adv Exp Med Biol|Gnaiger E (2003) Oxygen conformance of cellular respiration. A perspective of mitochondrial physiology. Adv Exp Med Biol 543: 39-55.]]<br />
# [[Gnaiger 2002 Biochem Soc Trans|Gnaiger E, Kuznetsov AV (2002) Mitochondrial respiration at low levels of oxygen and cytochrome c. Biochem Soc Trans 30: 252-258.]]<br />
# [[Gnaiger 2000 Life in the Cold|Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000b) Mitochondria in the cold. In: Life in the Cold. (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York: pp 431-442.]] – MiR05 as the basis of MiR06.<br />
# [[Gnaiger 1998 Biochim Biophys Acta | Gnaiger E, Lassnig B, Kuznetsov AV, Margreiter R (1998) Mitochondrial respiration in the low oxygen environment of the cell: Effect of ADP on oxygen kinetics. Biochim Biophys Acta 1365: 249-254.]]<br />
# [[Gnaiger 1998 J Exp Biol | Gnaiger E, Lassnig B, Kuznetsov AV, Rieger G, Margreiter R (1998) Mitochondrial oxygen affinity, respiratory flux control, and excess capacity of cytochrome c oxidase. J Exp Biol 201: 1129-1139.]]<br />
# [[Gueguen 2005 J Physiol | Gueguen N, Lefaucheur L, Ecolan P, Fillaut M, Herpin P (2005) Ca2+-activated myosin-ATPases, creatine and adenylate kinases regulate mitochondrial function according to myofibre type in rabbit. J Physiol 564: 723-735.]]<br />
# [[Hatefi 1962 J Biol Chem | Hatefi Y, Haavik AG, Fowler LR, Griffiths DE (1962) Studies on the electron transfer system. XLII. Reconstitution of the electron transfer system. J Biol Chem 237: 2661-2669.]]<br />
# [[Kuznetsov 2004 Am J Physiol Heart Circ Physiol | Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome c release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286: H1633–H1641.]] – Cytochrome ‘’c’’ test.<br />
# [[Lemieux 2006 Biochim Biophys Acta | Lemieux H, Garedew A, Blier PU, Tardif J-C, Gnaiger E (2006) Temperature effects on the control and capacity of mitochondrial respiration in permeabilized fibers of the mouse heart. Biochim Biophys Acta, EBEC Short Reports Suppl 14: 201-202.]]<br />
# [[Mitchell 1961 Nature | Mitchell P (1961) Coupling of phosphorylation to electron and hydrogen transfer by a chemi-osmotic type of mechanism. Nature 191: 144-148.]]<br />
# Mitchell P (1966) Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Glynn Research Ltd, Bodmin: 192 pp. - ''The Grey Book'' 1.<br />
# Mitchell P (1968) Chemiosmotic coupling and energy transduction. Glynn Research Ltd, Bodmin: 111 pp. - ''The Grey Book'' 2.<br />
# [[Mitchell 1967 Biochem J | Mitchell P, Moyle J (1967) Respiration-driven proton translocation in rat liver mitochondria. Biochem J 105: 1147-1162.]]<br />
# [[Mootha 1997 Am J Physiol | Mootha VK, Arai AE, Balaban RS (1997) Maximum oxidative phosphorylation capacity of the mammalian heart. Am J Physiol 272: H769-H775.]] – [Pi] <10 mM and [ADP] <0.4 mM limit OXPHOS in isolated heart mitochondria.<br />
# [[Nicholson 2012 Nature | Nicholson JK, Holmes E, Kinross JM, Darzi AW, Takats Z, Lindon JC (2012) Metabolic phenotyping in clinical and surgical environments. Nature 491: 384-392.]]<br />
# [[Owen 2002 J Biol Chem | Owen OE, Kalhan SC, Hanson RW (2002) The key role of anaplerosis and cataplerosis for citric acid cycle function. J Biol Chem 277: 30409-30412.]]<br />
# [[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]] - >90% saturation is reached only >5 mM ADP, yet few studies use such high [ADP] in permeabilized tissues and cells. - Oxygen limitation of respiration below air saturation.<br />
# [[Puchowicz 2004 Mitochondrion | Puchowicz MA, Varnes ME, Cohen BH, Friedman NR, Kerr DS, Hoppel CL (2004) Oxidative phosphorylation analysis: assessing the integrated functional activity of human skeletal muscle mitochondria – case studies. Mitochondrion 4: 377-385.]] - Cytochrome ''c'' test.<br />
# [[Rasmussen 2000 Mol Cell Biochem | Rasmussen UF, Rasmussen HN (2000) Human quadriceps muscle mitochondria: A functional characterization. Mol Cell Biochem 208: 37-44.]] - Cytochrome ''c'' test.<br />
# [[Renner 2003 Biochim Biophys Acta | Renner K , Amberger A, Konwalinka G, Gnaiger E (2003) Changes of mitochondrial respiration, mitochondrial content and cell size after induction of apoptosis in leukemia cells. Biochim Biophys Acta 1642: 115-123.]]<br />
# [[Rossignol 2003 Biochem J | Rossignol R, Faustin B, Rocher C, Malgat M, Mazat JP, Letellier T (2003) Mitochondrial threshold effects. Biochem J 370: 751-762.]]<br />
# [[Saks 1998 Mol Cell Biochem | Saks VA, Veksler VI, Kuznetsov AV, Kay L, Sikk P, Tiivel T, Tranqui L, Olivares J, Winkler K, Wiedemann F, Kunz WS (1998) Permeabilised cell and skinned fiber techniques in studies of mitochondrial function in vivo. Mol Cell Biochem 184: 81-100.]] - The apparent ''K''<sub>m</sub> for ADP increases up to 0.5 mM in some permeabilized muscle fibres.<br />
# [[Territo 2000 Am J Physiol Cell Physiol| Territo PR, Mootha VK, French SA, Balaban RS (2000) Ca<sup>2+</sup> activation of heart mitochondrial oxidative phosphorylation: role of the F0/F1-ATPase. Am J Physiol Cell Physiol 278: C423-C435.]]<br />
<br />
== Chapter 2. Mitochondrial pathways to Complex I: Respiratory substrate control with pyruvate, malate and glutamate ==<br />
<br />
=== References CI ===<br />
# Brandt U (2006) Energy converting NADH:quinone oxidoreductase (Complex I). Annu Rev Biochem 75: 69-92.<br />
# Brewer GJ, Jones TT, Wallimann T, Schlattner U (2004) Higher respiratory rates and improved creatine stimulation in brain mitochondria isolated with antioxidants. Mitochondrion 4: 49-57.<br />
# Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. III. The steady state. J Biol Chem 217: 409-427. - Substrate depletion in isolated mitochondria is achieved in State 2: ADP is added to induce a transient stimulation of oxygen flux based on oxidation of endogenous substrates. <br />
# Digerness SB, Reddy WJ (1976) The malate-aspartate shuttle in heart mitochondria. J Mol Cell Cardiol. 8: 779-785.<br />
# Duchen MR (2004) Roles of mitochondria in health and disease. Diabetes 53, Suppl 1: S96-S102. - Mitochondrial glutamate dehydrogenase is particularly active in astrocytes, preventing glutamate induced neurotoxicity.<br />
# [[Gnaiger 2009 Int J Biochem Cell Biol|Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837–1845.]]<br />
# Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97: 11080-11085. - Equilibrium ratio of malate to fumarate is 4.1.<br />
# Hildyard JCW, Halestrap AP (2003) Identification of the mitochondrial pyruvate carrier in ''Saccharomyces cerevidiae''. Biochem J 374: 607-611.<br />
# Johnson G, Roussel D, Dumas JF, Douay O, Malthiery Y, Simard G, Ritz P (2006) Influence of intensity of food restriction on skeletal muscle mitochondrial energy metabolism in rats. Am J Physiol Endocrinol Metab 291: E460-E467. - Uncoupling stimulates coupled OXPHOS respiration, PM<sub>''P''</sub>, by 14%.<br />
# Kemp RB, Hoare S, Schmalfeldt M, Bridge CM, Evans PM, Gnaiger E (1994) A thermochemical study of the production of lactate by glutaminolysis and glycolysis in mouse macrophage hybridoma cells. In What is Controlling Life? (Gnaiger E, Gellerich FN, Wyss M, eds) Modern Trends in BioThermoKinetics 3, Innsbruck Univ Press: 226-231. - Glutamate derived from hydrolyzation of glutamine is a very important aerobic substrate in cultured cells.<br />
# Lemasters JJ (1984) The ATP-to-oxygen stoichiometries of oxidative phosphorylation by rat liver mitochondria. J Biol Chem 259: 13123-13130. - Malonate added to inhibit the succinate-fumarate reaction exerts only a minor effect on liver mitochondrial respiration.<br />
# Maechler P, Carobbio S, Rubi B (2006) In beta-cells, mitochondria integrate and generate metabolic signals controlling insulin secretion. Int J Biochem Cell Biol 38: 696-709.<br />
# Messer JI, Jackman MR, Willis WT (2004) Pyruvate and citric acid cycle carbon requirements in isolated skeletal muscle mitochondria. Am J Physiol Cell Physiol 286: C565-572. - With malate alone and saturating [ADP] isolated rat skeletal muscle mitochondria respire at only 1.3% of OXPHOS capacity with pyruvate+malate. Pyruvate alone yields only 2.1% of OXPHOS capacity (State P) with PM.<br />
# Nicholls DG, Ferguson SJ (2002) Bioenergetics 3, Academic Press, London. 287 pp. - Carriers.<br />
# Ouhabi R, Boue-Grabot M, Mazat J-P (1994) ATP synthesis in permeabilized cells: Assessment of the ATP/O ratios in situ. In What is Controlling Life? (Gnaiger E, Gellerich FN, Wyss M, eds) Modern Trends in BioThermoKinetics 3, Innsbruck Univ Press: 141-144. - In fibroblasts, GM<sub>''P''</sub> supports a higher respiratory flux than PM<sub>''P''</sub>.<br />
# O’Donnell JM, Kudej RK, LaNoue KF, Vatner SF, Lewandowski ED (2004) Limited transfer of cytosolic NADH into mitochondria at high cardiac workload. Am J Physiol Heart Circ Physiol 286: H2237-H2242.<br />
# Puchowicz MA, Varnes ME, Cohen BH, Friedman NR, Kerr DS, Hoppel CL (2004) Oxidative phosphorylation analysis: assessing the integrated functional activity of human skeletal muscle mitochondria – case studies. Mitochondrion 4: 377-385. - OXPHOS with glutamate alone is 50% to 85% of respiration with glutamate+malate. Accumulation of fumarate inhibits succinate dehydrogenase and glutamate dehydrogenase (Caughey et al 1957; Dervartanian, Veeger 1964). - OXPHOS with glutamate+malate is identical or 10% higher than with pyruvate+malate.<br />
# Rasmussen UF, Rasmussen HN (2000) Human quadriceps muscle mitochondria: A functional characterization. Mol Cell Biochem 208: 37-44. - Uncoupling stimulates coupled OXPHOS respiration, PMP, by 15% in human skeletal muscle. OXPHOS with glutamate alone is 50% to 85% of respiration with glutamate+malate. - OXPHOS with glutamate+malate is identical or 10% higher than with pyruvate+malate.<br />
# Thomas et al (2004) - OXPHOS in human skeletal muscle for PM<sub>''P''</sub> is 25% higher than for GM<sub>''P''</sub>.<br />
# Winkler-Stuck K, Kirches E, Mawrin C, Dietzmann K, Lins H, Wallesch CW, Kunz WS, Wiedemann FR (2005) Re-evaluation of the dysfunction of mitochondrial respiratory chain in skeletal muscle of patients with Parkinson's disease. J Neural Transm 112: 499-518. - OXPHOS in human skeletal muscle for PM<sub>''P''</sub> is 16% higher than for GM<sub>''P''</sub>.<br />
<br />
=== Notes CI ===<br />
# Schwerzmann et al (1989) Proc Natl Acad Sci U S A 86: 1583-1587. “Of the substrates used here, pyruvate/malate activates the chain at complex I, glutamate/malate and succinate at complexes II and III, ..” - This consideration of glutamate+malate requires correction.<br />
# Ponsot et al (2005) J Cell Physiol 203: 479-486. (a) Respiration (State 3) in permeabilized fibres with malate alone gave 25-50% of the flux with pyruvate+malate. This most likely indicates a large content of endogenous mitochondrial substrates, which interfere to an unknown degree with the kinetics of respiration after addition of exogenous substrates. In such a study, the conventional initial depletion of endogenous substrates would be most important. (b) Maximal respiration rates in muscle should be evaluated at saturating or high Pi, since at a Pi concentration of 3 mM OXPHOS respiration is phosphate limited.<br />
# Hulbert et al (2006) J Comp Physiol B 176: 93-105. Addition of ‘sparking malate concentrations’. This term can probably be derived from the misconception that tricarboxylic acid cycle intermediates are conserved during respiration of isolated mitochondria. 380 µM malate (instead of mM concentrations) in conjunction with 2.4 mM pyruvate were used, which makes a comparison difficult between different tissues and different species: the low malate concentration may limit PMP flux at various degrees in the different sources of mitochondria, and GMP may support higher fluxes than PMP at tissue- and species-specific degrees.<br />
<br />
<br />
== Chapter 3. Mitochondrial pathways to Complex II. Glycerophosphate dehydrogenase and electrontransferring flavoprotein ==<br />
<br />
=== References CII ===<br />
# Capel F, Rimbert V, Lioger D, Diot A, Rousset P, Patureau Mirand P, Boirie Y, Morio B, Mosoni L (2005) Due to reverse electron transfer, mitochondrial H2O2 release increases with age in human vastus lateralis muscle although oxidative capacity is preserved. Mech Ageing Develop 126: 505-511. - With succinate alone OXPHOS is 30-40% lower than with succinate+rotenone in human skeletal muscle mitochondria.<br />
# Cecchini G (2003) Function and structure of Complex II of the respiratory chain. Annu Rev Biochem 72: 77-109.<br />
# Ernster L, Nordenbrand K (1967) Skeletal muscle mitochondria. In: Estabrook RW, Pullman ME (eds) Meth Enzymol: 86-94. – With succinate alone OXPHOS is 30-40% lower than with succinate+rotenone in rat skeletal muscle mitochondria.<br />
# Jackman MR, Willis WT (1996) Characteristics of mitochondria isolated from type I and type IIb skeletal muscle. Am J Physiol Cell Physiol 270: C673-678. - Glycerophosphate oxidation is 10-fold higher in rabbit gracilis mitochondria compared to soleus.<br />
# Lehninger AL (1970) Biochemistry. The molecular basis of cell structure and function Worth. 833 pp. - Oxaloacetate is a more potent competitive inhibitor of succinate dehydrogenase than malonate even at small concentration (p. 352).<br />
# Muller FL, Liu Y, Abdul-Ghani MA, Lustgarten MS, Bhattacharya A, Jang YC, Van Remmen H (2008) High rates of superoxide production in skeletal-muscle mitochondria respiring on both Complex I- and Complex II-linked substrates. Biochem J 409: 491–499. - Addition of malate inhibits superoxide production with succinate, probably due to the oxaloacetate inhibition of CII.<br />
# Rauchova H, Drahota Z, Rauch P, Fato R, Lenaz G (2003) Coenzyme Q releases the inhibitory effect of free fatty acids on mitochondrial glycerophosphate dehydrogenase. Acta Biochim Polonica 50: 405-413. - Glycerophosphate is an important substrate for respiration in brown adipose tissue mitochondria.<br />
# Rasmussen UF, Rasmussen HN (2000) Human quadriceps muscle mitochondria: A functional characterization. Mol Cell Biochem 208: 37-44. – Glycerophosphate oxidation is relatively slow.<br />
# Sun F, Huo X, Zhai Y, Wang A, Xu J, Su D, Bartlam M, Rao Z (2005) Crystal structure of mitochondrial respiratory membrane protein Complex II. Cell 121: 1043–1057.<br />
<br />
=== Notes CII === <br />
# Ponsot et al (2005) J Cell Physiol 203: 479-486. ‘.. the mitochondrial form of GPDH, which produces FADH2 within the mitochondrial matrix and provides electrons to Compoex II of the phosphorylation chain’. – The mitochondrial glycerophosphate dehydrogenase (GpDH), located on the outer side of the inner mitochondrial membrane, does not provide electrons to CII, but feeds electrons into the Q-cycle entirely independent of CII. FADH2 is not produced within the mitochondrial matrix. Electron transfer takes place from the mitochondrial inner membrane flavoprotein-linked glycerophosphate dehydrogenase to CoQ.<br />
# In the first edition of ‘Mitochondrial Pathways and Respiratory Control’ (2007), the term ‘electron transport’ is used synonymously for ‘electron transfer’.<br />
<br />
<br />
<br />
== Chapter 4. Mitochondrial pathways to Complexes I+II: Convergent electron transfer at the Q-Junction and additive effect of substrate combinations ==<br />
<br />
=== References CI+II ===<br />
# Aragonés J, Schneider M, Van Geyte K, Fraisl P, Dresselaers T, Mazzone M, Dirkx R, Zacchigna S, Lemieux H, Jeoung NH, Lambrechts D, Bishop T, Lafuste P, Diez-Juan A, K Harten S, Van Noten P, De Bock K, Willam C, Tjwa M, Grosfeld A, Navet R, Moons L, Vandendriessche T, Deroose C, Wijeyekoon B, Nuyts J, Jordan B, Silasi-Mansat R, Lupu F, Dewerchin M, Pugh C, Salmon P, Mortelmans L, Gallez B, Gorus F, Buyse J, Sluse F, Harris RA, Gnaiger E, Hespel P, Van Hecke P, Schuit F, Van Veldhoven P, Ratcliffe P, Baes M, Maxwell P, Carmeliet P (2008) Deficiency or inhibition of oxygen sensor Phd1 induces hypoxia tolerance by reprogramming basal metabolism. Nat Genet 40: 170-180. - OXPHOS analysis for phenotyping.<br />
# Bianchi C, Genova ML, Parenti Castelli G, Lenaz G (2004) The mitochondrial respiratory chain is partially organized in a supercomplex assembly: kinetic evidence using flux control analysis. J Biol Chem 279: 36562-36569.<br />
# Boushel R, Gnaiger E, Schjerling P, Skovbro M, Kraunsoe R, Flemming D (2007) Patients with Type 2 Diabetes have normal mitochondrial function in skeletal muscle. Diabetologia 50: 790-796.<br />
# Boushel R, Gnaiger E, Calbet JA, Gonzalez-Alonso J, Wright-Paradis C, Sondergaard H, Ara I, Helge JW, Saltin B (2011) Muscle mitochondrial capacity exceeds maximal oxygen delivery in humans. Mitochondrion 11: 303-307.<br />
# Capel F, Rimbert V, Lioger D, Diot A, Rousset P, Patureau Mirand P, Boirie Y, Morio B, Mosoni L (2005) Due to reverse electron transfer, mitochondrial H2O2 release increases with age in human vastus lateralis muscle although oxidative capacity is preserved. Mech Ageing Develop 126: 505-511. - CI+II substrate combination.<br />
# Chance B (1965) Reaction of oxygen with the respiratory chain in cells and tissues. J Gen Physiol 49: 163-188. - Glutamate+succinate as respiratory substrate combination, without comparison of flux with different substrates.<br />
# Costa LE, Boveris A, Koch OR, Taquini AC (1988) Liver and heart mitochondria in rats submitted to chronic hypobaric hypoxia. Am J Physiol Cell Physiol 255: C123-C129.<br />
# Digerness SB, Reddy WJ (1976) The malate-aspartate shuttle in heart mitochondria. J Mol Cell Cardiol. 8: 779-785.<br />
# Eberhart K, Rainer J, Bindreither D, Ritter I, Gnaiger E, Kofler R, Oefner PJ, Renner K (2011) Glucocorticoid-induced alterations in mitochondrial membrane properties and respiration in childhood acute lymphoblastic leukemia. Biochim Biophys Acta 1807: 719-725.<br />
# Estabrook R (1967) Mitochondrial respiratory control and the polarographic measurement of ADP:O ratios. Meth Enzymol 10: 41-47.<br />
# Garait B, Couturier K, Servais S, Letexier D, Perrin D, Batandier C, Rouanet J-L, Sibille B, Rey B, Leverve X, Favier R (2005) Fat intake reverses the beneficial effects of low caloric intake on skeletal muscle mitochondrial H2O2 production. Free Radic Biol Med 39: 1249–1261. - GM<sub>''P''</sub>/GMS<sub>''P''</sub> substrate control ratio in skeletal muscle of rats fed on various diets ranges from 0.7 to 0.8. <br />
# Garedew A, Lemieux H, Schachner T, Blier PU, Tardif J-C, Gnaiger E (2006) High excess capacity of cytochrome c oxidase in permeabilized fibers of the mouse heart. Biochim Biophys Acta, EBEC Short Reports Suppl 14: 167-168.<br />
# [[Gnaiger 2009 Int J Biochem Cell Biol|Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837–1845.]]<br />
# Gnaiger E, Wright-Paradis C, Sondergaard H, Lundby C, Calbet JA, Saltin B, Helge J, Boushel R (2005) High-resolution respirometry in small biopsies of human muscle: correlations with body mass index and age. Mitochondr Physiol Network 10.9: 14-15. http://www.mitophysiology.org/?gnaigere.<br />
# González-Flecha B, Cutrin JC, Boveris A (1993) Time course and mechanism of oxidative stress and tissue damage in rat liver subjected to in vivo ischemia-reperfusion. J Clin Invest 91: 456-464. - Respiration was measured in states GS<sub>''P''</sub> and GM<sub>''P''</sub>.<br />
# Gutman M, Coles CJ, Singer TP, Casida JE (1971) On the functional organization of the respiratory chain at the dehydrogenase-coenzyme Q junction. Biochemistry 10: 2036-2043.<br />
# Hansford RG, Hogue BA, Mildaziene V (1997) Dependence of H2O2 formation by rat heart mitochondria on substrate availability and donor age. J Bioenerg Biomembr 29: 89–95.<br />
# Hatefi Y, Haavik AG, Fowler LR, Griffiths DE (1962) Studies on the electron transfer system. XLII. Reconstitution of the electron transfer system. J Biol Chem 237: 2661-2669.<br />
# König T, Nicholls DG, Garland PB (1969) The inhibition of pyruvate and Ls(+)-isocitrate oxidation by succinate oxidation in rat liver mitochondria. Biochem J 114: 589-596.<br />
# Krebs HA (1935) CXCVII. Metabolism of amino-acids. III. Deamination of amino-acids. Biochem J 29: 1620-1644.<br />
# Kunz WS, Kudin A, Vielhaber S, Elger CE, Attardi G, Villani G (2000) Flux control of cytochrome c oxidase in human skeletal muscle. J Biol Chem 275: 27741-27745.<br />
# Kuznetsov AV, Clark JF, Winkler K, Kunz WS (1996) Increase of flux control of cytochrome c oxidase in copper-deficient mottled brindled mice. J Biol Chem 271: 283-288.<br />
# Kuznetsov AV, Strobl D, Ruttmann E, Königsrainer A, Margreiter R, Gnaiger E (2002) Evaluation of mitochondrial respiratory function in small biopsies of liver. Analyt Biochem 305: 186-194. - S(Rot) alone supports a higher flux than GM in liver mitocondria.<br />
# Kuznetsov AV, Winkler K, Kirches E, Lins H, Feistner H, Kunz WS (1997) Application of inhibitor titrations for the detection of oxidative phosphorylation defects in saponin-skinned muscle fibers of patients with mitochondrial diseases. Biochim Biophys Acta 1360: 142-150. - OXPHOS with glutamate+malate is identical or 10% higher than with pyruvate+malate.<br />
# LaNoue KF, Bryla J, Williamson JR (1972) Feedback interactions in the control of citric acid cycle activity in rat heart mitochondria. J Biol Chem 247: 667-679.<br />
# LaNoue KF, Schoolwerth AC (1979) Metabolite transport in mitochondria. Annu Rev Biochem 48: 871-922.<br />
# Lehninger AL (1970) Biochemistry. The molecular basis of cell structure and function Worth. 833 pp. - Electron transport chain.<br />
# Lemieux H, Garedew A, Blier PU, Tardif J-C, Gnaiger E (2006) Temperature effects on the control and capacity of mitochondrial respiration in permeabilized fibers of the mouse heart. Biochim Biophys Acta, EBEC Short Reports Suppl 14: 201-202.<br />
# [[Lemieux 2011 Int J Biochem Cell Biol|Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43: 1729–1738.]] - SUIT protocols.<br />
# Lenaz G, Genova ML (2009) Structural and functional organization of the mitochondrial respiratory chain: A dynamic super-assembly. Int J Biochem Cell Biol 41: 1750-1772.<br />
# Llesuy S, Evelson P, González-Flecha B, Peralta J, Carreras MC, Poderoso JJ, Boveris A (1994) Oxidative stress in muscle and liver of rats with septic syndrome. Free Radic Biol Med 16: 445-451. - GM<sub>''P''</sub>/GS<sub>''P''</sub> substrate control ratios are 0.8 and 0.7 in liver mitochondria (male Wistar and female Sprague-Dawley, respectively).<br />
# Mogensen M, Sahlin K (2005) Mitochondrial efficiency in rat skeletal muscle: influence of respiration rate, substrate and muscle type. Acta Physiol Scand 185: 229-236. - The flux control ratio of palmitoylcarnitine+malate/PM (OXPHOS) is 0.6 for mitochondria isolated from rat extensor digitorum longus muscle (mainly type II fibre type), but is 0.95 in rat soleus muscle (type I fibre type).<br />
# Muller FL, Liu Y, Abdul-Ghani MA, Lustgarten MS, Bhattacharya A, Jang YC, Van Remmen H (2008) High rates of superoxide production in skeletal-muscle mitochondria respiring on both Complex I- and Complex II-linked substrates. Biochem J 409: 491–499.<br />
# Nicholls DG, Ferguson SJ (2002) Bioenergetics 3, Academic Press, London. 287 pp. - Electron transport chain.<br />
# [[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]] - SUIT protocols.<br />
# [[Pesta 2011 Am J Physiol Regul Integr Comp Physiol|Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.]] - SUIT protocols.<br />
# Rasmussen HN, Rasmussen UF (1997) Small scale preparation of skeletal muscle mitochondria, criteria for integrity, and assays with reference to tissue function. Mol Cell Biochem 174: 55-60.<br />
# Rasmussen UF, Rasmussen HN (2000) Human quadriceps muscle mitochondria: A functional characterization. Mol Cell Biochem 208: 37-44. - OXPHOS with malate+palmitoylcarnitine is 0.60 of OXPHOS with GM.<br />
# Rasmussen UF, Rasmussen HN, Krustrup P, Quistorff B, Saltin B, Bangsbo J (2001) Aerobic metabolism of human quadriceps muscle: in vivo data parallel measurements on isolated mitochondria. Am J Physiol Endocrinol Metab 280: E301–E307.<br />
# Rossignol R, Faustin B, Rocher C, Malgat M, Mazat JP, Letellier T (2003) Mitochondrial threshold effects. Biochem J 370: 751-762.<br />
# Rumsey WL, Schlosser C, Nuutinen EM, Robiolio M, Wilson DF (1990) Cellular energetics and the oxygen dependence of respiration in cardiac myocytes isolated from adult rat. J Biol Chem 265. 15392-15399. - 2.5-fold increase of respiration in the LEAK state with GMS compared to GM.<br />
# Schägger H, Pfeiffer K (2001) The ratio of oxidative phosphorylation complexes I-V in bovine heart mitochondria and the composition of respiratory chain supercomplexes. J Biol Chem 276: 37861-37867.<br />
# Short KR, Bigelow ML, Kahl J, Singh R, Coenen-Schimke J, Raghavakaimal S, Nair KS (2005) Decline in skeletal muscle mitochondrial function with aging in humans. Proc Natl Acad Sci U S A 102: 5618 - 5623.<br />
# Simon J, van Spanning RJM, Richardson DJ (2008) The organisation of proton motive and non-proton motive redox loops in prokaryotic respiratory systems. Biochim Biophys Acta 1777: 1480–1490.<br />
# Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271: C2053-C2061.<br />
# Steinlechner-Maran R, Eberl T, Kunc M, Schröcksnadel H, Margreiter R, Gnaiger E (1997) Respiratory defect as an early event in preservation/reoxygenation injury in endothelial cells. Transplantation 63: 136-142.<br />
# Stadlmann S, Rieger G, Amberger A, Kuznetsov AV, Margreiter R, Gnaiger E (2002) H2O2-mediated oxidative stress versus cold ischemia-reperfusion: mitochondrial respiratory defects in cultured human endothelial cells. Transplantation 74: 1800-1803.<br />
# Sugano T, Oshino N, Chance B (1974). Mitochondrial functions under hypoxic conditions. The steady states of cytochrome ''c'' reduction and energy metabolism. Biochim Biophys Acta 347: 340-358. - Glutamate+succinate as respiratory substrate combination, without comparison of flux with different substrates.<br />
# Tonkonogi M, Walsh B, Tiivel T, Saks V, Sahlin K (1999) Mitochondrial funciton in human skeletal muscle is not impaired by high intensity exercise. Eur J Physiol 437: 562-568.<br />
# Torres NV, Mateo F, Sicilia J, Meléndez-Hevia E. (1988) Distribution of the flux control in convergent metabolic pathways: theory and application to experimental and simulated systems. Int J Biochem 20: 161-165.<br />
# Villani G, Attardi G (1997) In vivo control of respiration by cytochrome c oxidase in wild-type and mitochondrial DNA mutation-carrying human cells. Proc Natl Acad Sci U S A 94: 1166-1171.<br />
# Villani G, Greco M, Papa S, Attardi G (1998) Low reserve capacity of cytochrome c oxidase capacity in vivo in the respiratory chain of a variety of human cell types. J Biol Chem 273: 31829-31836.<br />
# [[Votion 2012 PLoS One|Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890.]] - SUIT protocols.<br />
# Wiedemann FR, Winkler K, Kuznetsov AV, Bartels C, Vielhaber S, Feistner H, Kunz WS (1998) Impairment of mitochondrial function in skeletal muscle of patients with amyotrophic lateral sclerosis. J Neurol Sci 156: 65-72. - - OXPHOS with glutamate+malate is identical or 10% higher than with pyruvate+malate.<br />
# Wilson DF, Rumsey WL, Green TJ, Vanderkooi J (1988). The oxygen dependence of mitochondrial oxidative phosphorylation measured by a new optical method for measuring oxygen concentration. J Biol Chem 263: 2712-2718. - Glutamate+succinate as respiratory substrate combination, without comparison of flux with different substrates.<br />
# Zoccarato F, Cavallini L, Bortolami S, Alexandre A (2007) Succinate modulation of H2O2 release at NADH:ubiquinone oxidoreductase (complex I) in brain mitochondria. Biochem J 406: 125–129. - CI+II substrate combination.<br />
<br />
:>> [[O2k-Publications:_Q-Junction_Effect]]<br />
<br />
=== Notes CI+II ===<br />
# Identical GM<sub>''P''</sub>/GS<sub>''P''</sub> or GM<sub>''P''</sub>/GMS<sub>''P''</sub> ratios of 0.7 are reported for isolated mitochondria (Rasmussen and Rasmussen 2000; Capel et al 2005) and permeabilized fibres (Kunz et al 2000). For a review see Gnaiger (2009).<br />
* '''Correction''': Occasionally 'electron gating' was erroneously spelled as 'gaiting': p. 32; legends to Fig. 1.5 (p. 15) and 4.2 (p. 39).<br />
<br />
== Chapter 5. Respiratory states, coupling control and coupling control ratios ==<br />
<br />
=== References Respiratory states ===<br />
# Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. I. Kinetics of oxygen utilization. J Biol Chem 217: 383-393.<br />
# Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. III. The steady state. J Biol Chem 217: 409-427.<br />
# Chance B, Williams GR (1956) The respiratory chain and oxidative phosphorylation. Adv Enzymol17: 65-134.<br />
# Estabrook R (1967) Mitochondrial respiratory control and the polarographic measurement of ADP:O ratios. Meth Enzymol 10: 41-47.<br />
# [[Gnaiger_2001_Respir Physiol|Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128: 277-297.]]<br />
# [[Gnaiger_1998_Biochim Biophys Acta|Gnaiger E, Lassnig B, Kuznetsov AV, Margreiter R (1998) Mitochondrial respiration in the low oxygen environment of the cell: Effect of ADP on oxygen kinetics. Biochim Biophys Acta 1365: 249-254.]] - Oxygen kinetics is different in the LEAK state without adenylates (''L''<sub>N</sub>) and State 4 (LEAK state with ATP, ''L''<sub>N</sub>).<br />
# [[Gnaiger 2000 Proc Natl Acad Sci U S A|Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97: 11080-11085.]]<br />
# König T, Nicholls DG, Garland PB (1969) The inhibition of pyruvate and Ls(+)-isocitrate oxidation by succinate oxidation in rat liver mitochondria. Biochem J 114: 589-596. - 3½ has been suggested to indicate an intermediate mitochondrial energy state somewhere between States 3 and 4. Would, therefore, State 4 be considered as being somewhere between State 3 and 5?<br />
# Nicholls DG, Ferguson SJ (2002) Bioenergetics 3, Academic Press, London. 287 pp. - ''Misconception on State 2.''<br />
<br />
:>> [[MitoPedia Glossary: Respiratory states]]<br />
<br />
== Chapter 6. Conversions of metabolic fluxes ==<br />
<br />
=== References Conversions ===<br />
<br />
<br />
# Brooks GA, Hittelman KJ, Faulkner JA, Beyer RE (1971) Temperature, skeletal muscle mitochondrial functions, and oxygen debt. Am J Physiol 220: 1053-1059.<br />
# Gnaiger E (1983) Symbols and units: Toward standardization. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York: 352-358.<br />
# Slater EC, Rosing J, Mol A (1973) The phosphorylation potential generated by respiring mitochondria. Biochim Biophys Acta 292: 534-553.<br />
<br />
<br />
<br />
== Apendix: Abbreviations ==<br />
<br />
=== A1. Respiratory coupling states and coupling control ratios ===<br />
<br />
=== A2. Substrates, uncouplers and inhibitors ===<br />
<br />
:>> [[List of substrates and metabolites]]<br />
:>> [[List of uncouplers]]<br />
:>> [[List of inhibitors]]<br />
<br />
<br />
== Follow-up ==<br />
<br />
* The 2007 Edition is cited in: <br />
:# [[Muller 2008 Biochem J|Muller FL et al (2008) High rates of superoxide production in skeletal-muscle mitochondria respiring on both Complex I- and Complex II-linked substrates. Biochem J 409: 491–499.]]<br />
:# [[Droese_2009_Biochim_Biophys_Acta|Dröse S, Hanley PJ, Brandt U (2009) Ambivalent effects of diazoxide on mitochondrial ROS production at respiratory chain complexes I and III. Biochim Biophys Acta 1790: 558-565.]]<br />
:# [[Hickey_2009_Am_J_Physiol_Cell_Physiol|Hickey AJ, Chai CC, Choong SY, de Freitas Costa S, Skea GL, Phillips AR, Cooper GJ (2009) Impaired ATP turnover and ADP supply depress cardiac mitochondrial respiration and elevate superoxide in nonfailing spontaneously hypertensive rat hearts. Am J Physiol Cell Physiol 297: C766–C774.]]<br />
:# Hoffman DL, Brookes PS (2009) Oxygen sensitivity of mitochondrial reactive oxygen species generation depends on metabolic conditions. J Biol Chem 284: 16236-16245.<br />
:# Sahlin K (2009) Control of lipid oxidation at the mitochondrial level. Appl Physiol Nutr Metab 34: 382-388:<br />
:# Bazil JN, Buzzard GT, Rundell AE (2010) Modeling mitochondrial bioenergetics with integrated volume dynamics. PLoS Comput Biol. 6: e1000632.<br />
:# Sivitz WI, Yorek MA (2010) Mitochondrial dysfunction in diabetes: from molecular mechanisms to functional significance and therapeutic opportunities. Antioxid Redox Signal 12: 537-577.<br />
:# Sivitz WI (2010) Mitochondrial Dysfunction in Obesity and Diabetes. US Endocrinology 6: 20-27.<br />
:# [[Iftikar 2010 JEMBE|Iftikar F, MacDonald JR, Hickey AJ (2010) Thermal limits of portunid crab heart mitochondria: Could more thermo-stable mitochondria advantage invasive species? JEMBE 395: 232-239.]]<br />
:# [[Mittal 2011 HPB (Oxford)|Mittal A, Hickey AJ, Chai CC, Loveday BP, Thompson N, Dare A, Delahunt B, Cooper GJ, Windsor JA, Phillips AR (2011) Early organ-specific mitochondrial dysfunction of jejunum and lung found in rats with experimental acute pancreatitis. HPB (Oxford) 13: 332-341.]]<br />
:# [[Pichaud 2011 Am J Physiol Regul Integr Comp Physiol|Pichaud N, Ballard JW, Tanguay RM, Blier PU (2011) Thermal sensitivity of mitochondrial functions in permeabilized muscle fibers from two populations of Drosophila simulans with divergent mitotypes. Am J Physiol Regul Integr Comp Physiol 301: R48-R59.]]<br />
:# [[Ye 2011 J Biol Chem|Ye F, Lemieux H, Hoppel CL, Hanson RW, Hakimi P, Croniger CM, Puchowicz M, Anderson VE, Fujioka H, Stavnezer E (2011) Peroxisome proliferator-activated receptor γ (PPARγ) mediates a Ski-induced shift from glycolysis to oxidative energy metabolism. J Biol Chem 286: 40013-40024.]]<br />
:# Cordeiro OD, Silva TS, Alves RN, Costas B, Wulff T, Richard N, de Vareilles M, Conceição LE, Rodrigues PM (2012) Changes in liver proteome expression of Senegalese sole (Solea senegalensis) in response to repeated handling stress. Mar Biotechnol (NY)14: 714-729.<br />
:# Everett MV, Antal CE, Crawford DL (2012) The effect of short-term hypoxic exposure on metabolic gene expression. J Exp Zool A Ecol Genet Physiol 317: 9-23.<br />
:# [[Miquel 2012 PLoS One|Miquel E, Cassina A, Martinez-Palma L, Bolatto C, Trias E, Gandelman M, Radi R, Barbeito L, Cassina P (2012) Modulation of astrocytic mitochondrial function by dichloroacetate improves survival and motor performance in inherited amyotrophic lateral sclerosis. PLoS One 7: e34776.]]<br />
:# [[De Paula Martins 2013 J Bioenerg Biomembr|de Paula Martins R, Glaser V, da Luz Scheffer D, de Paula Ferreira PM, Wannmacher CM, Farina M, de Oliveira PA, Prediger RD, Latini A (2013) Platelet oxygen consumption as a peripheral blood marker of brain energetics in a mouse model of severe neurotoxicity. J Bioenerg Biomembr 45: 449-457.]]<br />
:# [[Perry 2013 Diabetes|Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62: 1041-1053.]]<br />
:# Chu MJJ, Dare AJ, Bartlett ASJR, Phillips ARJ, Hickey AJ (2013) Effect of Hepatic Steatosis on Bioenergetic Function During Hepatic Ischemia-reperfusion: A Systematic Review. The Open Transplantation J 6: 10-32.<br />
:# [[Gispert_2013_Hum_Mol_Genet|Gispert S, Parganlija D, Klinkenberg M, Dröse S, Wittig I, Mittelbronn M, Grzmil P, Koob S, Hamann A, Walter M, Büchel F, Adler T, Hrabé de Angelis M, Busch DH, Zell A, Reichert AS, Brandt U, Osiewacz HD, Jendrach M, Auburger G (2013) Loss of mitochondrial peptidase Clpp leads to infertility, hearing loss plus growth retardation via accumulation of CLPX, mtDNA and inflammatory factors. Hum Mol Genet 22: 4871-4887.]]<br />
:# [[Hirzel 2013 J Recept Signal Transduct Res|Hirzel E, Lindinger PW, Maseneni S, Giese M, Rhein VV, Eckert A, Hoch M, Krähenbühl S, Eberle AN (2013) Differential modulation of ROS signals and other mitochondrial parameters by the antioxidants MitoQ, resveratrol and curcumin in human adipocytes. J Recept Signal Transduct Res 33:304-312.]]<br />
:# [[Cordero-Reyes_2014_J_Mol_Cell_Cardiol|Cordero-Reyes AM, Gupte AA, Youker KA, Loebe M, Hsueh WA, Torre-Amione G, Taegtmeyer H, Hamilton DJ (2014) Freshly isolated mitochondria from failing human hearts exhibit preserved respiratory function. J Mol Cell Cardiol doi: 10.1016/j.yjmcc.2013.12.029.]]<br />
<br />
* The 2010 Edition is cited in: <br />
:# Granados-Castro LF, Rodríguez-Rangel DS, Montaño M, Ramos C, Pedraza-Chaverri J (2013) Wood smoke exposure induces a decrease in respiration parameters and in the activity of respiratory complexes I and IV in lung mitochondria from guinea pigs. Environ Toxicol. doi: 10.1002/tox.21922.<br />
:# Maldonado F, Packard TT, Gómez M (2012) Understanding tetrazolium reduction and the importance of substrates in measuring respiratory electron transport activity. J Exp Marine Biol Ecol 434–435: 110–118.</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=File:BlueBook_openAccess.png&diff=58094File:BlueBook openAccess.png2014-03-19T14:45:22Z<p>Bauer Christiane: </p>
<hr />
<div></div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MitoGlobal_Events_2014&diff=58088MitoGlobal Events 20142014-03-19T12:53:37Z<p>Bauer Christiane: </p>
<hr />
<div>= Mitochondrial Global Network Events 2014 =<br />
[[Image:MitoGlobal.jpg|right|160px|link=http://www.bioblast.at/index.php/Mitochondrial_Global_Network|MitoGlobal]]<br />
<br />
<br />
<br />
'''MitoGlobal Events 2014''' - listed by '''[[Mitochondrial Global Network]]''' ->> [[MitoGlobal Events 2015]] - >> [[MitoGlobal Events]]<br />
<br />
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* '''Please inform us about upcoming mitochondria events''' --> send an e-mail to: [mailto:verena.laner@oroboros.at verena.laner@oroboros.at]<br />
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<br />
{| class="wikitable" border="1"<br />
|-<br />
! scope="col" width="80px" | '''2014'''<br />
! scope="col" width="150px" | '''Link'''<br />
! scope="col" width="770px" | '''Event'''<br />
! scope="col" width="150px" | '''Location'''<br />
|-<br />
| '''March'''<br />
|-<br />
|<br />
:19 to 20<br />
| [http://www.healthtech.com/Mitochondrial-Targeting/ Mitochondrial Targeting]<br />
| Targeting Mitochondrial Dysfunction & Toxicity<br />
| Boston, US<br />
|-<br />
|<br />
:24 to 31<br />
| [http://www.ntnu.edu/isb/enjoy-the-cold Enjoy the Cold]<br />
| Course: Enjoy the Cold - Adaption and risk assessment of human activities in cold environments (Norwegian University of Science and Technology)<br />
| Spitsbergen, NO<br />
|-<br />
|<br />
:27<br />
|[[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?IOC84_alaska O2k-Workshop IOC87]<br />
| OROBOROS O2k-Workshop on [[High-Resolution Respirometry]] (HRR) and O2k-Fluorometry integrated in [http://www.ntnu.edu/isb/enjoy-the-cold Enjoy the Cold]<br />
|Spitsbergen, NO <br />
|-<br />
| '''April'''<br />
|-<br />
|<br />
: 02 <br />
|[http://sigs.nih.gov/mito/Pages/default.aspx Mitochondrial Interest Group]<br />
|A Mitochondrial Etiology of Metabolic and Degenerative Diseases Cancer and Ageing - lecture by Dr. Wallace and Transformational Medicine in the Mitochondrial Age Symposium<br />
| Bethesda, US<br />
|-<br />
|<br />
: 07 to 12<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-workshops O2k-Fluorometry Workshop IOC88]<br />
| OROBOROS O2k-Workshop on [[High-Resolution Respirometry]] (HRR) and O2k-Fluorometry<br />
| Schroecken, AT<br />
|-<br />
|<br />
: 26 to 30<br />
| <br />
| Symposium for Experimental Biology<br />
| San Diego, CA, US<br />
|-<br />
|<br />
:30 Apr to 03 May<br />
| [http://www.esci.eu.com/meetings/ ESCI]<br />
| European Society for Clinical Investigation<br />
| Utrecht, NL<br />
|-<br />
| '''May'''<br />
|-<br />
|<br />
: 01 to 02<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-workshops O2k-Fluorometry Workshop IOC89]<br />
| [[High-Resolution Respirometry]] (HRR) and O2k-Fluorometry at [https://www.scripps.edu/index.html The Scripps Research Institute]<br />
| La Jolla, CA, US <br />
|-<br />
|<br />
:04 to 07<br />
| [http://meetochondrie.ibgc.cnrs.fr/ Meet''O''chondrie 2014]<br />
| 7<sup>th</sup> Colloque Meet''O''chondrie<br />
| Evian, Haute-Savoie, FR<br />
|-<br />
|<br />
: 07 to 10<br />
| [http://oxyclubcalifornia.org/OCC2014/index.php OCC2014]<br />
| Oxygen Club of California 2014 World Congress<br />
| Davis, CA, US<br />
|-<br />
|<br />
: 14 to 16<br />
| [http://hq.prosite.be:85/NICC/2013pre/dna-forensics-2014 DNA Forensics 2014]<br />
| 9<sup>th</sup> International '''Y-chromosome workshop''' & 6<sup>th</sup> International '''EMPOP meeting'''<br />
| Brussels, BE<br />
|-<br />
|<br />
: 25 to 31<br />
| [http://www.ismm2014.org/Pages/default.aspx ISMM2014]<br />
| World Congress on High Altitude Medicine and Physiology<br />
| Bolzano, IT<br />
|- <br />
|<br />
: 27<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-workshops O2k-Fluorometry Workshop IOC]<br />
| ISMM Congress O2k-Workshop on [[High-Resolution Respirometry]] (HRR) and O2k-Fluorometry<br />
| Bolzano, IT <br />
|-<br />
| '''June'''<br />
|-<br />
|<br />
: 04 to 07<br />
| [http://www.umdf.org/site/c.piKYL1PHLtF/b.4864979/k.C003/Home.htm UMDF2014]<br />
| Mitochondrial Medicine 2014<br />
| Pittsburgh, PA, US<br />
|-<br />
|<br />
: 15 to 19<br />
| [[Image:Euromit2014.jpg|100px]][http://www.euromit2014.org/ EUROMIT 2014]<br />
| EUROMIT 2014 - International Meeting on Mitochondrial Pathology<br />
| Tampere, FI<br />
|-<br />
|<br />
: 28 Jun to 03 Jul<br />
| [http://www.isott2014.org/ ISOTT 2014]<br />
| 42nd Meeting of the International Society on Oxygen Transport to Tissue<br />
| London, UK<br />
|-<br />
| '''July'''<br />
|-<br />
|<br />
: 06 to 10<br />
| [http://www.respiratory-science.org/ International Congress of Respiratory Science]<br />
| 3rd International Congress of Respiratory Science<br />
| Bad Honnef, DE<br />
|-<br />
|<br />
: 12 to 17<br />
| [http://www.ebec2014.org/ EBEC2014]<br />
| 18th European Bioenergetics Conference - EBEC 2014<br />
| Lisbon, PT<br />
|-<br />
| '''August'''<br />
|-<br />
|<br />
: <br />
|<br />
|<br />
|<br />
|-<br />
| '''September'''<br />
|-<br />
|<br />
: 08 to 12<br />
| [[Image:MiPsocietyLOGO.JPG|left|90px|link=http://www.mitophysiology.org|MiPsociety]]'''[[MiP2014]]''' <br />
| [[Image:IUBMB-logo.gif|left|60px|link=http://www.iubmb.org|IUBMB - ''International Union of Biochemistry and Molecular Biology'']]10<sup>th</sup> MiP''conference'': Joint IUBMB/MiP Symposium on Mitochondrial Physiology - a Point/Counterpoint Meeting <br />
| Obergurgl, AT<br />
|-<br />
|<br />
: 12 to 16<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-Workshops O2k-Fluorometry Workshop IOC]<br />
| OROBOROS post-conference Workshop<br />
| Obergurgl, AT<br />
|-<br />
| '''October'''<br />
|-<br />
|<br />
: 01 to 03<br />
| [http://www.cell-symposia-metabolic-diseases.com/ Cell Symposia: Systems Approach to Metabolic Diseases]<br />
| Cell Symposia: Systems Approach to Metabolic Diseases<br />
| Chicago, IL, US<br />
|-<br />
|<br />
: 07 to 12<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-workshops O2k-Fluorometry Workshop IOC]<br />
| OROBOROS O2k-Workshop on [[High-Resolution Respirometry]] (HRR)<br />
| Schroecken, AT<br />
|<br />
|-<br />
| '''November'''<br />
|-<br />
|<br />
: 13 to 14 <br />
| [http://www.targeting-mitochondria.com/ Targeting Mitochondria 2014]<br />
| 5th World Congress on Targeting Mitochondria<br />
| Berlin, DE<br />
|-<br />
| '''December'''<br />
|-<br />
|<br />
: 04 to 04 <br />
| SMRM14<br />
| 4th Annual Conference of the Society for Mitochondrial Research and Medicine (SMRM), Mitochondrial Biology and Disease<br />
| Kolkata, IN<br />
|- <br />
|}<br />
<br />
Previous events in 2014:<br />
<br />
{| class="wikitable" border="1"<br />
|-<br />
! scope="col" width="80px" | '''2014'''<br />
! scope="col" width="150px" | '''Link'''<br />
! scope="col" width="770px" | '''Event'''<br />
! scope="col" width="150px" | '''Location'''<br />
|-<br />
| '''January'''<br />
|-<br />
|<br />
: 04 to 07<br />
|[[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?IOC84_alaska O2k-Workshop IOC84]<br />
| OROBOROS O2k-Workshop on [[High-Resolution Respirometry]] (HRR) and O2k-Fluorometry integrated in Alaska expedition - [http://www.oroboros.at/index.php?id=racing-dogs_alaska Racing dogs at Iditarod]<br />
|Big Lake, Alaska, US <br />
|-<br />
|<br />
: 27 to 30<br />
| [http://www.tifr.res.in/~dbsconf/mito2014 mito2014]<br />
| Mitochondria, Metabolism and Energetics, 38th Mahabaleshwar Seminar<br />
| Mahabaleshwar, IN <br />
|-<br />
|<br />
: 27 to 30<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-workshops O2k-Fluorometry Workshop IOC85]<br />
| OROBOROS O2k-Workshop on [[High-Resolution Respirometry]] (HRR) and O2k-Fluorometry integrated in [http://www.tifr.res.in/~dbsconf/mito2014 mito2014], IOC85, 38th Mahabaleshwar Seminar<br />
| Mahabaleshwar, IN<br />
|-<br />
|<br />
: 31<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-workshops O2k-Fluorometry Workshop IOC86]<br />
| OROBOROS O2k-Workshop on [[High-Resolution Respirometry]] (HRR) and O2k-Fluorometry<br />
| Mumbai, IN<br />
|-<br />
| '''February'''<br />
|-<br />
|<br />
: 08 <br />
| [http://www.mitoaction.org/laconference Mitochondrial Disease Clinical Conference]<br />
| Mitochondrial Disease Clinical Conference LA<br />
| Los Angeles, CA, US<br />
|-<br />
|<br />
: 09 to 14<br />
| [http://www.grc.org/programs.aspx?year=2014&program=oxygenrad GRC]<br />
| Oxygen Radicals Gordon Research Conference<br />
| Ventura, CA, US <br />
|-<br />
|<br />
: 18 to 23<br />
| [http://www.keystonesymposia.org/index.cfm?e=web.meeting.program&meetingid=1266 Keystone Symposia]<br />
| Mitochondrial Dynamics and Physiology (Q5) joint with the meeting on The Chemistry and Biology of Cell Death (Q6)<br />
| Santa Fe, New Mexico, US <br />
|-<br />
|<br />
: 26<br />
| [http://www.abcam.com/MitochondriaFEB abcam Mitochondria meeting]<br />
| Mitochondria, Energy Metabolism and Cancer meeting<br />
| London, UK <br />
|}<br />
<br />
[[Category:Mitochondrial Global Network]]</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MitoGlobal_Events_2014&diff=58085MitoGlobal Events 20142014-03-19T12:47:04Z<p>Bauer Christiane: </p>
<hr />
<div>= Mitochondrial Global Network Events 2014 =<br />
[[Image:MitoGlobal.jpg|right|160px|link=http://www.bioblast.at/index.php/Mitochondrial_Global_Network|MitoGlobal]]<br />
<br />
<br />
<br />
'''MitoGlobal Events 2014''' - listed by '''[[Mitochondrial Global Network]]''' ->> [[MitoGlobal Events 2015]] - >> [[MitoGlobal Events]]<br />
<br />
<br />
* '''Please inform us about upcoming mitochondria events''' --> send an e-mail to: [mailto:verena.laner@oroboros.at verena.laner@oroboros.at]<br />
<br />
<br />
<br />
<br />
<br />
{| class="wikitable" border="1"<br />
|-<br />
! scope="col" width="80px" | '''2014'''<br />
! scope="col" width="150px" | '''Link'''<br />
! scope="col" width="770px" | '''Event'''<br />
! scope="col" width="150px" | '''Location'''<br />
|-<br />
| '''March'''<br />
|-<br />
|<br />
:19 to 20<br />
| [http://www.healthtech.com/Mitochondrial-Targeting/ Mitochondrial Targeting]<br />
| Targeting Mitochondrial Dysfunction & Toxicity<br />
| Boston, US<br />
|-<br />
|<br />
:24 to 31<br />
| [http://www.ntnu.edu/isb/enjoy-the-cold Enjoy the Cold]<br />
| Course: Enjoy the Cold - Adaption and risk assessment of human activities in cold environments (Norwegian University of Science and Technology)<br />
| Spitsbergen, NO<br />
|-<br />
|<br />
:27<br />
|[[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?IOC84_alaska O2k-Workshop IOC87]<br />
| OROBOROS O2k-Workshop on [[High-Resolution Respirometry]] (HRR) and O2k-Fluorometry integrated in [http://www.ntnu.edu/isb/enjoy-the-cold Enjoy the Cold]<br />
|Spitsbergen, NO <br />
|-<br />
| '''April'''<br />
|-<br />
|<br />
: 02 <br />
|[http://sigs.nih.gov/mito/Pages/default.aspx Mitochondrial Interest Group]<br />
|A Mitochondrial Etiology of Metabolic and Degenerative Diseases Cancer and Ageing - lecture by Dr. Wallace and Transformational Medicine in the Mitochondrial Age Symposium<br />
| Bethesda, US<br />
|-<br />
|<br />
: 07 to 12<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-workshops O2k-Fluorometry Workshop IOC88]<br />
| OROBOROS O2k-Workshop on [[High-Resolution Respirometry]] (HRR) and O2k-Fluorometry<br />
| Schroecken, AT<br />
|-<br />
|<br />
: 26 to 30<br />
| <br />
| Symposium for Experimental Biology<br />
| San Diego, CA, US<br />
|-<br />
|<br />
:30 Apr to 03 May<br />
| [http://www.esci.eu.com/meetings/ ESCI]<br />
| European Society for Clinical Investigation<br />
| Utrecht, NL<br />
|-<br />
| '''May'''<br />
|-<br />
|<br />
: 01 to 02<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-workshops O2k-Fluorometry Workshop IOC89]<br />
| [[High-Resolution Respirometry]] (HRR) and O2k-Fluorometry at [https://www.scripps.edu/index.html The Scripps Research Institute]<br />
| La Jolla, CA, US <br />
|-<br />
|<br />
:04 to 07<br />
| [http://meetochondrie.ibgc.cnrs.fr/ Meet''O''chondrie 2014]<br />
| 7<sup>th</sup> Colloque Meet''O''chondrie<br />
| Evian, Haute-Savoie, FR<br />
|-<br />
|<br />
: 07 to 10<br />
| [http://oxyclubcalifornia.org/OCC2014/index.php OCC2014]<br />
| Oxygen Club of California 2014 World Congress<br />
| Davis, CA, US<br />
|-<br />
|<br />
: 14 to 16<br />
| [http://hq.prosite.be:85/NICC/2013pre/dna-forensics-2014 DNA Forensics 2014]<br />
| 9<sup>th</sup> International '''Y-chromosome workshop''' & 6<sup>th</sup> International '''EMPOP meeting'''<br />
| Brussels, BE<br />
|-<br />
|<br />
: 25 to 31<br />
| [http://www.ismm2014.org/Pages/default.aspx ISMM2014]<br />
| World Congress on High Altitude Medicine and Physiology<br />
| Bolzano, IT<br />
|- <br />
|<br />
: 27<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-workshops O2k-Fluorometry Workshop IOC]<br />
| ISMM Congress O2k-Workshop on [[High-Resolution Respirometry]] (HRR) and O2k-Fluorometry<br />
| Bolzano, IT <br />
|-<br />
| '''June'''<br />
|-<br />
|<br />
: 04 to 07<br />
| [http://www.umdf.org/site/c.piKYL1PHLtF/b.4864979/k.C003/Home.htm UMDF2014]<br />
| Mitochondrial Medicine 2014<br />
| Pittsburgh, PA, US<br />
|-<br />
|<br />
: 15 to 19<br />
| [[Image:Euromit2014.jpg|100px]][http://www.euromit2014.org/ EUROMIT 2014]<br />
| EUROMIT 2014 - International Meeting on Mitochondrial Pathology<br />
| Tampere, FI<br />
|-<br />
|<br />
: 28 Jun to 03 Jul<br />
| [http://www.isott2014.org/ ISOTT 2014]<br />
| 42nd Meeting of the International Society on Oxygen Transport to Tissue<br />
| London, UK<br />
|-<br />
| '''July'''<br />
|-<br />
|<br />
: 06 to 10<br />
| [http://www.respiratory-science.org/ International Congress of Respiratory Science]<br />
| 3rd International Congress of Respiratory Science<br />
| Bad Honnef, DE<br />
|-<br />
|<br />
: 12 to 17<br />
| [http://www.ebec2014.org/ EBEC2014]<br />
| 18th European Bioenergetics Conference - EBEC 2014<br />
| Lisbon, PT<br />
|-<br />
| '''August'''<br />
|-<br />
|<br />
: <br />
| <br />
| <br />
| <br />
|-<br />
| '''September'''<br />
|-<br />
|<br />
: 08 to 12<br />
| [[Image:MiPsocietyLOGO.JPG|left|90px|link=http://www.mitophysiology.org|MiPsociety]]'''[[MiP2014]]''' <br />
| [[Image:IUBMB-logo.gif|left|60px|link=http://www.iubmb.org|IUBMB - ''International Union of Biochemistry and Molecular Biology'']]10<sup>th</sup> MiP''conference'': Joint IUBMB/MiP Symposium on Mitochondrial Physiology - a Point/Counterpoint Meeting <br />
| Obergurgl, AT<br />
|-<br />
|<br />
: 12 to 16<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-Workshops O2k-Fluorometry Workshop IOC]<br />
| OROBOROS post-conference Workshop<br />
| Obergurgl, AT<br />
|-<br />
| '''October'''<br />
|-<br />
|<br />
: 01 to 03<br />
| [http://www.cell-symposia-metabolic-diseases.com/ Cell Symposia: Systems Approach to Metabolic Diseases]<br />
| Cell Symposia: Systems Approach to Metabolic Diseases<br />
| Chicago, IL, US<br />
|-<br />
|<br />
: 07 to 12<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-workshops O2k-Fluorometry Workshop IOC]<br />
| OROBOROS O2k-Workshop on [[High-Resolution Respirometry]] (HRR)<br />
| Schroecken, AT<br />
|<br />
|-<br />
| '''November'''<br />
|-<br />
|<br />
: 13 to 14 <br />
| [http://www.targeting-mitochondria.com/ Targeting Mitochondria 2014]<br />
| 5th World Congress on Targeting Mitochondria<br />
| Berlin, DE<br />
|-<br />
| '''December'''<br />
|-<br />
|<br />
: 04 to 04 <br />
| SMRM14<br />
| 4th Annual Conference of the Society for Mitochondrial Research and Medicine (SMRM), Mitochondrial Biology and Disease<br />
| Kolkata, IN<br />
|- <br />
|}<br />
<br />
Previous events in 2014:<br />
<br />
{| class="wikitable" border="1"<br />
|-<br />
! scope="col" width="80px" | '''2014'''<br />
! scope="col" width="150px" | '''Link'''<br />
! scope="col" width="770px" | '''Event'''<br />
! scope="col" width="150px" | '''Location'''<br />
|-<br />
| '''January'''<br />
|-<br />
|<br />
: 04 to 07<br />
|[[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?IOC84_alaska O2k-Workshop IOC84]<br />
| OROBOROS O2k-Workshop on [[High-Resolution Respirometry]] (HRR) and O2k-Fluorometry integrated in Alaska expedition - [http://www.oroboros.at/index.php?id=racing-dogs_alaska Racing dogs at Iditarod]<br />
|Big Lake, Alaska, US <br />
|-<br />
|<br />
: 27 to 30<br />
| [http://www.tifr.res.in/~dbsconf/mito2014 mito2014]<br />
| Mitochondria, Metabolism and Energetics, 38th Mahabaleshwar Seminar<br />
| Mahabaleshwar, IN <br />
|-<br />
|<br />
: 27 to 30<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-workshops O2k-Fluorometry Workshop IOC85]<br />
| OROBOROS O2k-Workshop on [[High-Resolution Respirometry]] (HRR) and O2k-Fluorometry integrated in [http://www.tifr.res.in/~dbsconf/mito2014 mito2014], IOC85, 38th Mahabaleshwar Seminar<br />
| Mahabaleshwar, IN<br />
|-<br />
|<br />
: 31<br />
| [[Image:OROBOROS-solo.png|left|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]][http://www.oroboros.at/?O2k-workshops O2k-Fluorometry Workshop IOC86]<br />
| OROBOROS O2k-Workshop on [[High-Resolution Respirometry]] (HRR) and O2k-Fluorometry<br />
| Mumbai, IN<br />
|-<br />
| '''February'''<br />
|-<br />
|<br />
: 08 <br />
| [http://www.mitoaction.org/laconference Mitochondrial Disease Clinical Conference]<br />
| Mitochondrial Disease Clinical Conference LA<br />
| Los Angeles, CA, US<br />
|-<br />
|<br />
: 09 to 14<br />
| [http://www.grc.org/programs.aspx?year=2014&program=oxygenrad GRC]<br />
| Oxygen Radicals Gordon Research Conference<br />
| Ventura, CA, US <br />
|-<br />
|<br />
: 18 to 23<br />
| [http://www.keystonesymposia.org/index.cfm?e=web.meeting.program&meetingid=1266 Keystone Symposia]<br />
| Mitochondrial Dynamics and Physiology (Q5) joint with the meeting on The Chemistry and Biology of Cell Death (Q6)<br />
| Santa Fe, New Mexico, US <br />
|-<br />
|<br />
: 26<br />
| [http://www.abcam.com/MitochondriaFEB abcam Mitochondria meeting]<br />
| Mitochondria, Energy Metabolism and Cancer meeting<br />
| London, UK <br />
|}<br />
<br />
[[Category:Mitochondrial Global Network]]</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=MiP2014&diff=58080MiP20142014-03-19T08:53:11Z<p>Bauer Christiane: /* MiP2014 preliminary participants */</p>
<hr />
<div>[[Image:IUBMB-logo.gif|left|100px|link=http://www.iubmb.org|IUBMB - ''International Union of Biochemistry and Molecular Biology'']]<br />
[[Image:MiPsocietyLOGO.JPG|right|200px|link=http://www.mitophysiology.org|MiPsociety]]<br />
[http://www.mitophysiology.org/?MiP2014 '''<big>MiP2014 - 10<sup>th</sup> MiP''conference'': Joint IUBMB/MiP Symposium on Mitochondrial Physiology - a Point/Counterpoint Meeting</big>''']<br />
<br />
'''''International Union of Biochemistry and Molecular Biology''''' and '''Mitochondrial Physiology Society'''<br />
<br />
Obergurgl, Tyrol, Austria. 2014-Sep-08 to 12<br />
== MiP2014 - a point/counterpoint meeting ==<br />
<br />
Mitochondrial research has recently undergone a dramatic renaissance, due to numerous discoveries revealing its central role in cell death, disease pathology, aging, thermogenesis, oxidative stress, cell signalling, cellular regulation and cell bioenergetics. The aim of the small symposium is to bring together experts in mitochondrial research spanning from the biochemical and molecular level to the interactions within the cell. In addition to the latest scientific findings, new methodological developments in measuring mitochondrial function and dysfunction are presented. A point/counterpoint format is chosen to discuss selected hot topics and some fundamental methodological and conceptual issues.<br />
<br />
The expected attendance is max. 100 participants. ->> Compare [[MiP2013 Programme]]<br />
Abstracts and presentations: refer to [http://www.chem.qmul.ac.uk/iubmb/ IUBMB nomenclature]; >> [[MitoPedia Glossary: Respiratory states|MitoPedia Glossary: '''Respiratory states''']]<br />
<br />
== Programme structure ==<br />
<br />
In each of the 12 sessions a point/counterpoint introduction will be given by invited keynote speakers to provide a balanced overview and promote discussions. Short oral presentations are selected from abstracts, including student presentations. Rapid platform statements will introduce posters which contribute to a specific session. Posters are up throughout the meeting, and poster discussions are integrated into the platform sessions.<br />
<br />
=== A. Mitochondrial respiratory control === <br />
* A1. mtDNA, mt-phenotype and disease – is there biochemical and molecular-genetic evidence to explain observed associations? <br />
* A2. Fission and fusion, mitochondrial structure and function - is there a tight connection? <br />
* A3. Mitochondrial density and normalization of respiration – is there a general biochemical or molecular marker? <br />
* A4. Biochemical reserve capacity, respiratory control ratios and coupling efficiency – are conventional concepts inconsistent?<br />
<br />
=== B. Experimental protocols in mitochondrial biochemistry === <br />
* B1. Incubation media for mt-preparations and experimental temperature in functional studies of mammalian mitochondria – guided by concept or tradition? <br />
* B2. Intracellular oxygen pressure versus oxygen regimes in studies of ROS production and respiration - are mitochondria sources or sinks of ROS?<br />
* B3. Quantitative measurement of mt-membrane potential - potentiometric/fluorometric. <br />
* B4. Instrumental platforms in mitochondrial physiology – industrial presentations.<br />
<br />
=== C. Towards quality assurance === <br />
* C1. Do blood cells versus muscle biopsies provide valid models for diagnosis of mitochondrial pathologies? <br />
* C2. Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
* C3. From availability and quality control of chemicals to mitochondrial respiratory protocols – who controls? <br />
* C4. Standards for the diagnosis of mitochondrial pathologies – a challenge for collaboration with mitochondrial organizations?<br />
Particularly in view of Subtopic C, it may be attractive for mitochondrial organizations to send and support delegates. The conference will, therefore, provide a unique forum for international networking, and may inspire new national groups to be formed in contact with established mitochondrial organizations:<br />
>> '''[[MitoGlobal_Organizations]]'''<br />
<br />
== MiP2014 preliminary participants ==<br />
<br />
in progress<br />
<br />
{| class="wikitable"<br />
|-<br />
! Name !! Institution !! Preliminary title <br />
|-<br />
| [[Ali SS|Ali Sameh S]]||Zewail City for Science and Technology, ET||Oxygen-consuming and ROS-producing activities in synaptosomes.<br />
|-<br />
| [[Bauer CM|Bauer Christiane Maria]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Bernardi P|Bernardi Paolo]]||University of Padova, IT||<br />
|-<br />
| [[Borutaite_V|Borutaite Vilmante]]||Lithuanian University of Health Sciences, Kaunas, LT||Controversies in testing integrity of mitochondrial membranes.<br />
|-<br />
| [[Brown D|Brown David]]||East Carolina University, NC, US||<br />
|-<br />
| [[Contreras-Ferrat A|Contreras-Ferrat Ariel]]||University of Chile, CL||<br />
|-<br />
| [[Coen P|Coen Paul]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Dohlmann T|Dohlmann Tine]]||University of Copenhagen, DK||<br />
|-<br />
| [[Elmer E|Elmer Eskil]]||Wallenberg Neuroscience Center, Lund, SE||Ex vivo respiratory capacity of human blood cells - a SUITable application of the O2k Oxygraph / A novel pharmacological approach to counteract metabolic crisis<br />
|-<br />
| [[Felding-Habermann B|Felding-Habermann Brunhilde]]||The Scripps Research Institute, La Jolla, CA, US||Mitochondrial functions in breast cancer progression.<br />
|-<br />
| [[Goodpaster BH|Goodpaster Bret]]||Translational Research Institute for Metabolism and Diabetes, Orlando, FL, US||<br />
|-<br />
| [[Gnaiger E|Gnaiger Erich]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Hahn D|Hahn Dagmar]]||Inselspital Bern, CH||<br />
|-<br />
| [[Hand SC|Hand Steven C]]||Louisiana State University, LA, US||Integrity of mitochondrial preparations – is a consensus possible on quality control criteria?<br />
|-<br />
| [[Harrison DK|Harrison David K]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Hepple_RT| Hepple Russel T.]]||McGill University, Montreal, CA||Mitochondrial involvement in aging muscle: cause and effect(or).<br />
|-<br />
| [[Hey-Mogensen M|Hey-Mogensen Martin]]||Novo Nordisk A/S Diabetes NBEs & Obesity Biology, Måløv, DK||<br />
|-<br />
| [[Jana Prado F|Jana Prado Fabian]]||University of Chile, CL||<br />
|-<br />
| [[Kraunsoe R|Kraunsoe Regitze]]||University of Copenhagen, DK||<br />
|-<br />
| [[Koopman WJ|Koopman Werner J]]||Radboud University Nijmegen Medical Centre, NL||Redox regulation of mitochondrial dynamics.<br />
|-<br />
| [[Kowaltowski AJ|Kowaltowski Alicia J.]]||University of Sao Paulo, BR||<br />
|-<br />
| [[Laner V|Laner Verena]]||OROBOROS INSTRUMENTS, Innsbruck, AT||<br />
|-<br />
| [[Larsen S|Larsen Steen]]||University of Copenhagen, DK||Normalization of respiration.<br />
|-<br />
| [[Larsson NG|Göran-Larsson Nils]]||Max Planck Institute for the Biology of Ageing, Cologne, DE||Mouse models with mtDNA mutations.<br />
|-<br />
| [[Ojuka E|Ojuka Edward]]||University of Cape Town, ZA||Fission and fusion, mitochondrial structure and function - is there a tight connection?<br />
|-<br />
| [[Perry CG|Perry Christopher G]]||York University, Ontario, CA||Re-visiting critical experimental parameters to control when measuring respiratory kinetics in permeabilized muscle fibres<br />
|-<br />
| [[Pesta D|Pesta Dominik]]||Yale University School of Medicine, CT, US||<br />
|- <br />
| [[Picard_M|Picard Martin]]||University of Pennsylvania, PA, US||Quantitative Regulation of Nuclear Gene Expression by Mitochondrial DNA<br />
Heteroplasmy<br />
|-<br />
| [[Pecina P|Pecina Petr]]||Academy of Sciences of the Czech Republic, Prague, CZ||The use of lymphocytes for diagnostics of mitochondrial disorders.<br />
|-<br />
| [[Purhonen J|Purhonen Janne]]||University Helsinki, FI||<br />
|-<br />
| [[Ristow M|Ristow Michael]]||ETH Zurich, CH||Promoting health and lifespan by increasing mitochondrial ROS formation.<br />
|-<br />
| [[Shirihai O|Shirihai Orian]]||The Shirihai Lab at Boston University, MA, US||<br />
|-<br />
| [[Skulachev VP|Skulachev Vladimir]]||Lomonosov Moscow State University, Moscow, RU||Mitochondria are sources of ROS rather than sinks: Physiological effects of mitochondria-targeted antioxidants.<br />
|-<br />
| [[Starkov AA|Starkov Anatoly]]||Weill Medical College Cornell University, NY, US||<br />
|-<br />
| [[Stiban J|Stiban Johnny]]||Birzeit University, Ramallah, PS||Very long chain ceramides interfere with C16-ceramide-induced channel formation: A plausible mechanism for regulating the initiation of intrinsic apoptosis.<br />
|-<br />
| [[Zorzano A|Zorzano Antonio]]||Universitat de Barcelona, ES||Metabolic implications of mitofusin 2 dysfunction.<br />
|}<br />
<br />
== Venue ==<br />
<br />
The symposium will be hosted in the '''University Center in Obergurgl''' 100 km from Innsbruck. The closest airport is in Innsbruck. Participants arriving at Munich airport will take the train or an airport shuttle to Innsbruck. We provide support in arranging the transfer between Munich and Innsbruck. A bus transfer is organized between Innsbruck and Obergurgl.<br />
<br />
The Obergurgl University Center is located at an altitude of 1940 meters above sea-level. The historical buildings, carefully refurbished and equipped with the latest technical systems, can accommodate around 70 persons in 43 bedrooms and includes conference rooms, the Alpine Research Center, laboratory, sports medicine, lounges and the restaurant. All rooms are equipped with an Internet connection. Additional participants are accommodated in smaller guest houses in 5 minutes walking distance. Meals are served for all participants in the University Center. Two conference rooms for a total of 200 persons and three small rooms for round table discussions are available. There is enough space for poster presentations and instrument exhibitions. The cosy bar and lounge area with an open fire place adds to the familiar atmosphere and supports the interactive spirit of the symposium.<br />
<br />
The small village of Obergurgl is situated in an impressive landscape at about 1900 m above sea level in the Ötz valley. It is surrounded by mountains, offering an exceptional view to the Ötz valley glaciers. During summer time the area is famous for hiking and for skiing in winter time. On the doorstep: one of Europe´s finest Biosphere Parks. The unique surroundings offer the possibility of combining scientific discussions while exploring the alpine environment during the famous “walks and talks”. The informal atmosphere of the workshop encourages also students and young post-docs to participate actively in scientific discussions and to develop future collaborations.<br />
<br />
University Centre Obergurgl, Gaisbergweg 5, 6456 Obergurgl, Tyrol, Austria.<br />
Tel.: +43 (0) 512 507 37201 | Fax: +43 (0) 512 507 37400 | Email: obergurgl@uibk.ac.at <br />
<br />
== International programme committee ==<br />
The international programme committee will provide scientific input by active participation, nominate further invited speakers to ensure balanced point/counterpoint introductions, and select the abstracts as a basis for participation (restricted number of participants), for oral 10+5 min presentations, and for flash presentations.<br />
<br />
Chair:<br />
* [[Gnaiger E|Gnaiger Erich]], Medical University Innsbruck, AT. - erich.gnaiger@mitophysiology.org<br />
Members:<br />
* [[Ali SS|Ali Sameh S]], Helmy Institute of Medical Sciences, Giza, Egypt<br />
* [[Borutaite V|Borutaite Vilma]], Lithuanian University of Health Sciences Kaunas, LT<br />
* [[Neurocon|Chakrabarti Sasanka]], Institute of Post-graduate Medical Education & Research, Kolkatta, India<br />
* [[Chinese Society of Mitochondrial Research and Medicine|Chen Quan]], Chinese Academy of Sciences, Nankai University, Beijing <br />
* [[Dela F|Dela Flemming]], University of Copenhagen, DK <br />
* [[Duchen MR|Duchen Michael R]], University College London, UK<br />
* [[Elmer E|Elmér Eskil]], Lund University, SE <br />
* [[Hand SC|Hand Steven]], Louisiana State University, LA, US<br />
* [[Hickey AJ|Hickey Anthony J]], University of Auckland, NZ<br />
* [[Koopman WJ|Koopman Werner J]], Radboud Univ Nijmegen Medical Centre, NL<br />
* [[Tanaka M|Tanaka Masashi]], Toyko Metropolitan Institute of Gerontology, JP<br />
* [[Neufer PD|Neufer P Darrell]], East Carolina University, Greenville, NC, US<br />
* [[Ojuka E|Ojuka Edward]], University of Cape Town, South Africa<br />
* [[Pecina P|Pecina Petr]], Academy of Sciences of the Czech Republic, CZ<br />
* [[Perry CG|Perry Christopher G]], York University, Toronto, Canada<br />
* [[Rossignol R|Rossignol Rodrigue]], Université Victor Segalen-Bordeaux, FR<br />
* [[Vercesi AE|Vercesi Anibal E]], State University of Campinas, Sao Paulo, Brazil<br />
* [[Taiwan Society for Mitochondrial Research and Medicine|Wei Yau-Huei]], Mackay Medical College, New Taipei City, Taiwan<br />
* [[Zorzano A|Zorzano Antonio]], Universitat de Barcelona, Spain<br />
<br />
== Information for invited speakers==<br />
<br />
MiP2014 will be held at the University Centre Obergurgl, with maximum capacity of 124 persons and a price per person per night of € 65.- including a shared double room, breakfast, and dinner. MiP''conferences'' are organized as small meetings facilitating communication between senior and junior participants. In the tradition of MiP''conferences'', we are asking invited speakers to cooperate and try to receive local support for joining us at the prestigious Mitochondrial Physiology Conference (limited to 124 participants). Support will be provided to students and young investigators.<br />
<br />
== Post-conference O2k-Workshop ==<br />
<br />
A post-conference workshop is organized on the same venue, for hands-on training in mitochondrial respiratory physiology, introducing the combined measurement of high-resolution respirometry and fluorometry (OROBOROS INSTRUMENTS). Since participants come from all-over-the-world, including many students, the combination of the symposium with the workshop makes travelling more economical for the maximum of 24 workshop-participants. The post-conference workshop is financially completely separate from the symposium.<br />
<br />
>> '''[[MitoGlobal_Events_2014]]'''<br />
<br />
<br />
<br />
<br />
<br />
[[Category:MitoPedia]]<br />
[[Category:All]]</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=O2k-Titration_Set&diff=58079O2k-Titration Set2014-03-19T08:34:06Z<p>Bauer Christiane: </p>
<hr />
<div>{{Mainpage orobopedia}}<br />
{{Product<br />
|description=The '''Titration Set''' consists of 6x 10 µl, 6x 25 µl, and 2x 50 µl Hamilton glass syringes, and 3 spare plungers for the 10 µl syringes. It is a basic HRR-Accessory included in the [[O2k-Core]] Unit.<br />
|product id=20820-01<br />
|product type=O2k, O2k-Module, O2k-Core, Microsyringe, Catalogue<br />
|info=[[MiPNet09.12 O2k-Titrations]]<br />
|product image=[[Image:Hamilton Syringes for Manual Titration.jpg|right|180px|link=http://www.bioblast.at/index.php/Titration_Set]]<br />
}}<br />
<br />
<br />
<br />
== The '''Titration Set''' ''consists of'' ==<br />
<br />
:6 [[Microsyringe\10 mm3 51/0.13 mm]] 10 mm<sup>3</sup><br />
:6 [[Microsyringe\25 mm3 51/0.15 mm]] 25 mm<sup>3</sup> <br />
:2 [[Microsyringe\50 mm3 51/0.15 mm]] 50 mm<sup>3</sup><br />
:3 [[Plunger\10 mm3]] for 10 mm<sup>3</sup> syringe<br />
<br />
<br />
== '''Handling of the syringes''' ==<br />
===General information===<br />
Use separate syringes for substrates, uncoupler and inhibitors.<br />
<br />
Colour code of syringes:<br />
* Substrate: white<br />
* Uncoupler: blue<br />
* Inhibitors: red<br />
* Digitonin: yellow<br />
<br />
===Preparation of syringes before experiment===<br />
* Prepare syringes according to SUIT protocol.<br />
* Prepare a rack with three 50 ml falcons for washing procedure:<br />
:* First rinse (distilled water) <br />
:* Distilled water<br />
:* 100% EtOH <br />
:* Prepare beaker for waste<br />
* Washing procedure:<br />
:* Discard residual chemical into the beaker<br />
:* Plunge syringe in 'first rinse' falcon (distilled water) and wipe it with a small kimwipe. Take care to rinse not only the needle itself but also the needle base<br />
:* Wash syringe three times in falcon filled with distilled water or 100% EtOH (depends on whether reagents were dissolved in distilled water or EtOH).<br />
:* Syringes used for AmR or CCCP: wash twice with DMSO (flush syringe surface with distilled water between washing steps).<br />
<br />
===Syringes in use===<br />
* Hold the syringe on the top to prevent warming of the glass.<br />
* Fill the syringe above desired mark in order to have a little bit of excess. <br />
* Take care not to produce bubbles when filling the syringe – this causes inaccuracies in measurement.<br />
* Fill the syringe not too fast but steady. Pumping is also ok.<br />
* Before titrating the chemical into the chamber: carefully press plunger and check if a small drop appears on top of the needle <br />
* Be swift when titrating in the chamber, especially when titrating EtOH due to its volatile nature. <br />
* After titrating in the chamber, discard the residual chemical into the beaker and rinse the outside of the syringe with distilled water (plunging in the falcon labelled 'first rinse') and dab it gently using small kimwipes. <br />
* Store syringes in 15 ml glass epprouvettes during the experiment in used solvent (distilled water or EtOH, use a separate epprouvette for every syringe). <br />
* Caution: take care to specifically rinse the syringe used for ADP (with MgCl2) IMMEDIATELY after use (on the surface but more importantly inside 3 times with distilled water) as a precipitate may form and cause blocking of the needle.<br />
<br />
===Preparation of syringes after experiment and storage===<br />
* Take care of the washing order: first substrate-, then uncoupler- and finally inhibitor syringes.<br />
* Wash three times with last used solvent, take care again to rinse also the needle base, rinse three times with EtOH 100% (use a small beaker).<br />
* Store syringes in dry condition. <br />
<br />
<br />
<u>Note:</u><br> <br />
When using a syringe with a chemical different from the usually assigned one then a washing step with distilled water is crucial to prevent medium contamination by carry-over!<br />
<br />
===Further useful information===<br />
* Visit also: [http://www.hamiltoncompany.com/Syringes/ Hamilton] and [http://www.hamiltoncompany.com/Syringes/techInfo/PDFs/L20058_care_use_guide.pdf Hamilton info pdf]<br />
* Eliminate rugh edges with 3M™ Wetordry™ Paper Sheet P1000 by gentle rubbing<br />
* Use a cleaning wire for the needle. If a syringe is clocked , sometimes it is also helpful to fill it from the back with EtOH or H2O and try to press the plunger gentle down.</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=O2k-Titration_Set&diff=58078O2k-Titration Set2014-03-19T08:27:08Z<p>Bauer Christiane: </p>
<hr />
<div>{{Mainpage orobopedia}}<br />
{{Product<br />
|description=The '''Titration Set''' consists of 6x 10 µl, 6x 25 µl, and 2x 50 µl Hamilton glass syringes, and 3 spare plungers for the 10 µl syringes. It is a basic HRR-Accessory included in the [[O2k-Core]] Unit.<br />
|product id=20820-01<br />
|product type=O2k, O2k-Module, O2k-Core, Microsyringe, Catalogue<br />
|info=[[MiPNet09.12 O2k-Titrations]]<br />
|product image=[[Image:Hamilton Syringes for Manual Titration.jpg|right|180px|link=http://www.bioblast.at/index.php/Titration_Set]]<br />
}}<br />
<br />
<br />
<br />
== The '''Titration Set''' ''consists of'' ==<br />
<br />
:6 [[Microsyringe\10 mm3 51/0.13 mm]] 10 mm<sup>3</sup><br />
:6 [[Microsyringe\25 mm3 51/0.15 mm]] 25 mm<sup>3</sup> <br />
:2 [[Microsyringe\50 mm3 51/0.15 mm]] 50 mm<sup>3</sup><br />
:3 [[Plunger\10 mm3]] for 10 mm<sup>3</sup> syringe<br />
<br />
<br />
== '''Handling of the syringes''' ==<br />
===General information===<br />
Use separate syringes for substrates, uncoupler and inhibitors.<br />
<br />
Colour code of syringes:<br />
* Substrate: white<br />
* Uncoupler: blue<br />
* Inhibitors: red<br />
* Digitonin: yellow<br />
<br />
===Preparation of syringes before experiment===<br />
* Prepare syringes according to SUIT protocol.<br />
* Prepare a rack with three 50 ml falcons for washing procedure:<br />
:* First rinse (distilled water) <br />
:* Distilled water<br />
:* 100% EtOH <br />
:* Prepare beaker for waste<br />
* Washing procedure:<br />
:* Discard residual chemical into the beaker<br />
:* Plunge syringe in 'first rinse' falcon (distilled water) and wipe it with a small kimwipe. Take care to rinse not only the needle itself but also the needle base<br />
:* Wash syringe three times in falcon filled with distilled water or 100% EtOH (depends on whether reagents were dissolved in distilled water or EtOH).<br />
:* Syringes used for AmR or CCCP: wash twice with DMSO (flush syringe surface with distilled water between washing steps).<br />
<br />
===Syringes in use===<br />
* Hold the syringe on the top to prevent warming of the glass.<br />
* Fill the syringe above desired mark in order to have a little bit of excess. <br />
* Take care not to produce bubbles when filling the syringe – this causes inaccuracies in measurement.<br />
* Fill the syringe not too fast but steady/constant. Pumping is also ok.<br />
* Before titrating the chemical into the chamber: carefully press plunger and check if a small drop appears on top of the needle <br />
* Be swift when titrating in the chamber, especially when titrating EtOH due to its volatile nature. <br />
* After titrating in the chamber, discard the residual chemical into the beaker and rinse the outside of the syringe with distilled water (plunging in the falcon labelled 'first rinse') and dab it gently using small kimwipes. <br />
* Store syringes in 15 ml glass epprouvettes during the experiment in used solvent (distilled water or EtOH, use a separate epprouvette for every syringe). <br />
* Caution: take care to specifically rinse the syringe used for ADP (with MgCl2) IMMEDIATELY after use (on the surface but more importantly inside 3 times with distilled water) as a precipitate may form and cause blocking of the needle.<br />
<br />
===Preparation of syringes after experiment and storage===<br />
* Take care of the washing order: first substrate-, then uncoupler- and finally inhibitor syringes.<br />
* Wash three times with last used solvent, take care again to rinse also the needle base, rinse three times with EtOH 100% (use a small beaker).<br />
* Store syringes in dry condition. <br />
<br />
<br />
<u>Note:</u><br> <br />
When using a syringe with a chemical different from the usually assigned one then a washing step with distilled water is crucial to prevent medium contamination by carry-over!<br />
<br />
===Further useful information===<br />
* Visit also: [http://www.hamiltoncompany.com/Syringes/ Hamilton] and [http://www.hamiltoncompany.com/Syringes/techInfo/PDFs/L20058_care_use_guide.pdf Hamilton info pdf]<br />
* Eliminate rugh edges with 3M™ Wetordry™ Paper Sheet P1000 by gentle rubbing<br />
* Use a cleaning wire for the needle. If a syringe is clocked , sometimes it is also helpful to fill it from the back with EtOH or H2O and try to press the plunger gentle down.</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=O2k-Fluo_LED2-Module&diff=58064O2k-Fluo LED2-Module2014-03-17T14:50:11Z<p>Bauer Christiane: </p>
<hr />
<div>{{Mainpage orobopedia}}<br />
{{Product<br />
|description=<big>The '''O2k-Fluorescence LED2-Module'''</big> is an amperometric add-on module to the [[O2k-Core]], adding a new dimension to [[HRR]]. Optical sensors are inserted through the front window of the O2k-glass chambers, for measurement of '''hydrogen peroxide production''' ([[Amplex red]]), '''ATP production''' ([[Magnesium green]]), '''mt-membrane potential''' ([[Safranin]]), '''Ca<sup>2+</sup>''' ([[Calcium green]]), and numerous other applications open for O2k-user innovation. <br />
The O2k-Fluorescence LED2-Module consists of optical sensors for both [[O2k-Chamber]]s (LEDs for green and blue excitation), optical filters, [[Fluorescence-Control Unit]] for regulation of light intensity, data input into the [[O2k-Main Unit]], and the updated '''DatLab''' software.<br />
|product id=12100-01<br />
|product type=O2k, O2k-Module, MultiSensor, Catalogue<br />
|info=[http://www.oroboros.at/?O2k-Fluorescence O2k-Fluorescence@OROBOROS], [[Oxygraph-2k]], [[G MiPNet17.05 O2k-Fluorescence LED2-Module]]<br />
|product image=[[Image:O2k-Fluorescence LED2-Module.JPG|180px|right|link=http://www.bioblast.at/index.php/O2k-Fluorescence_LED2-Module]]<br />
}}<br />
__TOC__<br />
== The '''O2k-Fluorescence LED2-Module''' ''consists of'' ==<br />
{{#ask: mainlabel=Title|[[Category:Products]] | [[Product type::O2k-Fluorescence LED2-Module]]<br />
| ?description<br />
| ?product id<br />
| ?product image<br />
|sort=product id<br />
|order=ascending<br />
}}<br />
<br />
== O2k-Fluorometry Workshops ==<br />
* 2013 September 19-23. [http://www.oroboros.at/index.php?id=1534 '''International O2k-Fluorometry Workshop IOC79, Obergurgl, Austria'''].<br />
* 2012 March [http://www.oroboros.at/?IOC66 O2k-Fluorometry Workshop, March 2012, Innsbruck, Austria.] <br />
* [http://www.oroboros.at/?O2k-Workshops upcoming Workshops]<br />
<br />
<br />
== O2k-Manual: O2k-Fluorescence LED2-Module ==<br />
>> [[G MiPNet17.05 O2k-Fluorescence LED2-Module|O2k-Manual: O2k-Fluorescence LED2-Module]]<br />
: Open Innovation: The '''''Manual'' for the O2k-Fluorescence LED2-Module''' evolves as a guided tour through the '''O2k-Catalogue: O2k-Fluorescence LED2-Module'''.<br />
<br />
=== Scope of Application ===<br />
There are many different fluorometric measurement techniques. The O2k-Fluorescence LED2-Module is designed for measurements that are typically performed by placing a (stirred) cuvette with the sample into a (spectro)fluormeter. Protocols for these cuvette based measurements can be implemented with the O2k-Fluorescence LED2-Module, adding simultaneous measurement of respiration and the monitoring of oxygen concentration to the protocol. The scope of possible applications is further defined by the excitation wavelengths provided by the sensors Fluorescence-Sensor Green and Fluorescence-Sensor Blue and the availability of suitable emission filters.<br />
Therefore, classical fluorometric measurements can be “translated” to an O2k equipped with the O2k-Fluorescence LED2-Module if the following conditions are met:<br />
* the fluorescence measurement was done in a cuvette using a (spectro)fluorometer<br />
* one of the fluorophores listed below under [[O2k-Fluorescence_LED2-Module#Application-specific_settings]] was used OR excitation and emission wavelength of the fluorophore are suitable for use with the O2k-Fluorescence LED2-Module. Please contact Oroboros Instruments if in doubt about the latter point. <br />
<br />
The O2k-Fluorescence LED2-Module is not designed to fulfill the functions of other devises used in fluorometric measurements e.g. fluorescence microscopes, flow cytometers, etc..<br />
<br />
=== Setup of the O2k-Fluorescence LED2-Module ===<br />
# [[Fluorescence-Control_Unit#Setup_of_the_O2k-Fluorescence_LED2-Module|Setup of the O2k-Fluorescence LED2-Module]]<br />
# [[Fluorescence-Sensor#Select_the_Fluorescence-Sensors|Selecting a Fluorescence Sensor]]'''<br />
# [[Filter-Cap#Mounting_a_Filter-Cap|Mounting a Filter-Cap]]<br />
# [[Fluorescence-Sensor#Connect_Fluorescence-Sensor_to_O2k|Connect Fluorescence-Sensor to O2k-Main unit]]<br />
<br />
=== Electronic settings ===<br />
# [[Fluorescence-Control_Unit#Power_on|Power on]]<br />
# [[Fluorescence-Control_Unit#Control_of_LED-intensity|LED-intensity]]<br />
# [[Fluorescence-Control_Unit#Gain|Amplification or Gain]]<br />
<br />
=== Application-specific settings ===<br />
The light intensity of the LED ([[Fluorescence-Control_Unit#Control_of_LED-intensity|LED-intensity]]) and the signal amplification ([[Fluorescence-Control_Unit#Amplification|Gain]]) can be adjusted in a wide range. The table suggests initial values, which may be optimised for specific applications. <br />
<br />
{| class="wikitable"<br />
|-<br />
! Application !!Sensor!! Filter set !! Light intensity (polarization voltage) - ''Note a'' !! Gain<br />
|- <br />
| [[Amplex® UltraRed]]||[[Fluorescence-Sensor Green]] || [[ Filter Set AmR| AmR]] || 100 - 500 || 1000 <br /><br />
(at light intensity = 100)<br />
|- <br />
| [[TMRM]]||[[Fluorescence-Sensor Green]] || [[ Filter Set AmR| AmR]] || 500 - 1000 || 1000 <br />
|-<br />
| [[Safranin]] ||[[Fluorescence-Sensor Blue]]|| [[Filter Set Saf|Saf]] ||200 for [safranin] > 2 µM; 500 or higher for [safranin] < 2 µM|| 1000<br />
|-<br />
| [[Magnesium green]]||[[Fluorescence-Sensor Blue]]|| [[Filter Set MgG / CaG| MgG / CaG]] || 300|| ''Note b''<br />
|-<br />
| [[Calcium green]]||[[Fluorescence-Sensor Blue]]|| [[Filter Set MgG / CaG| MgG / CaG]] |||| ''Note b''<br />
|}<br />
<br />
* ''Note a'': Set the polarization voltage [mV] for the amperometric channel (Amp) in the DatLab menu [O2k-MultiSensor \ O2k Control \ Amp polarisation voltage]. Divide the polarisation voltage [mV] by 100 to obtain the current [mA] through the LED. For simple operation instructions, it is sufficient to refer to the polarization settings selected in DatLab.<br />
* ''Note b'': The amplification for Magnesium green(R) and Calcium green(R) depends on the concentration of the fluorophore, which varies drastically between different applications. Therefore, no general recommendation is given for the appropriate gain. <br />
<br />
<br />
=== [[DatLab]]-Analysis ===<br />
<br />
==== Additional Templates for Fluorescence ====<br />
A DatLab Template file for fluorescence applications can be downloaded [http://www.oroboros.at/?O2k-fluorescence @OROBOROS].<br />
See [[MiPNet12.07]] for instructions how to import a DatLab Template files. <br />
<br />
<br />
==== Observing the fluorescence signal ====<br />
Use Graph Layout “A Amp” to display “Amp Raw Signal”<br />
<br />
[[Image:DL5_graph_select_plots_amp.PNG|thumb|250px|alt=Check Amp raw signal form Graph/Select Plots to display the fluorometric signal| Graph / Select plots ]]'''Graph layout''': Three plots are available in DatLab based on the recorded signal: '''Amp Raw Signal''', '''Amp Calibrated''', and '''Amp Slope'''<nowiki>. These plots can be selected from the drop-down lines and displayed with their check boxes either on the Y1 or Y2 [Graph layout / Select Plots].</nowiki> <br />
<br />
<br />
<br />
'''Amp Raw Signal''' displays the raw voltage (including amplification) as recorded by the Oxygraph at a given gain setting. <br />
<br />
'''Amp Calibrated''' is the signal after calibration with the parameters set in the O2k-MultiSensor Calibration window. <br />
<br />
'''Amp slope''' is the time derivative of the '''calibrated''' signal, multiplied by '''1000''', in units [m(conc. Unit during calibration)/s], so if the signal was calibrated in µM [nmol/ml] the unit of the slope is pmol/(s ml). To obtain the slope of the raw signal check the appropriate box in the calibration window ([[DatLab]] 5.1.0.130 and above).<br />
<br />
'''Graphs''' can be generated to display oxygen and fluorescence data, or several graphs can be added to display oxygen and fluorescence data separately. Layout templates are provided, which can be modified and saved as appropriate. All graph settings can be saved as user-defined layouts [[MiPNet12.07]].<br />
<br />
==== The calibration window ====<br />
If the active plot is "Amp" or "Amp slope" using either [MultiSensor]/[MultiSensor Calibration], or <nowiki>[Ctrl+F5] </nowiki>, pressing the right mouse key on the plot legend will open the calibration window for the Amp channel, see [[MiPNet12.08]].<br />
<br />
'''Setting properties of calibrated signal plot and slope plot''' <br />
[[Image:DL5_1_0_130_Amp_calibration_user_settings.PNG|400px|right]]<br />
* Type the desired plot name in the field "Name for Amp-channel". Avoid long names.<br />
* Choose the desired unit for the calibrated signal from the drop down menu beside "Unit". Default: "µM". <br />
* Choose the desired factor for slope calculation from the drop down menu beside "Slope factor". Default: 1000. The correct unit for the slope will be set by DatLab depending on the chosen unit for the calibrated signal and the factor for slope calculation. Changing the factor will recalculate the values for the slope plot correctly.<br />
* To calculate the slope based on the [[raw signal]], activate the checkbox "Calculate from raw signal". If viewing the raw (uncalibrated signal) it is recommended to use this option.<br />
* Click "Calibrate and Copy to Clipboard" to apply all changes.<br />
<br />
'''Two point linear calibration:'''<br />
To perform a simple two-point linear calibration of the "Amp channel"<br />
* select "Amp" as active plot<br />
* place two marks on to stable regions of the signal corresponding to two known concentrations<br />
* open the calibration window<br />
* use the drop down menus in the "Select Mark" column to to select the two marks of known concentration<br />
* enter the concentrations in the column labeled "Amp concentration"; express the concentrations in the unit selected as described above<br />
* Click "Calibrate and Copy to Clipboard" to apply all changes. <br />
<br />
<br />
'''Multiple point linear calibration:'''<br />
<br />
# <nowiki>Calculate the regression of raw voltage [V] as a function of concentration [µM] in a spreadsheet program.</nowiki><br />
# Note slope and intercept; add the two values to get the value for "slope+intercept"<br />
# Open the MultiSensor calibration window<br />
# Enter the data matrix shown below<br />
# Press Calibrate and Copy to clipboard.<br />
[[file:DL5_Amp_multpoint_calib.PNG|300px|right]]<br />
{| class="wikitable"<br />
|-<br />
! c [µM] !! Select Mark !! Raw Signal [V]<br />
|-<br />
| 1 || leave empty || slope + intercept<br />
|-<br />
| 0 || leave empty|| intercept<br />
|}<br />
<br />
<br />
<br />
<br />
<br />
<nowiki>Alternative: If the regression was done for c in [µM] against raw voltage in [V] the following data matrix has to be entered:</nowiki><br />
<br />
{| class="wikitable"<br />
|-<br />
! c [µM] !! Select Mark !! Raw Signal [V]<br />
|-<br />
| slope + intercept || leave empty || 1<br />
|-<br />
| intercept || leave empty|| 0<br />
|}<br />
<br />
=== Performing an experiment ===<br />
The O2k-Fluorescence Sensors can be inserted into the chamber of the O2k at any time when using the O2k. <br />
<br />
# Set up the instrument as described [[O2k-Fluorescence_LED2-Module#Setup_of_the_O2k-Fluorescence_LED2-Module above.]]<br />
# Switch off the chamber light [F10].<br />
# Observe the "Amp raw signal" and "slope Amp" as described above. It will take some minutes for the sensor to reach a constant temperature and therefore a stable signal. Therefore, it is advisable to insert the fluorescence sensors early in the set up of the instrument. However, they can be removed at any time to visually check the chamber. When a sensor is removed for a short time only it will reach a stable temperature and therefore a stable signal very soon after re-inserting it into the window of the O2k chamber.<br />
# Set up your experiment as usually for respiration experiments. Remove the fluorescence sensors whenever necessary. The time for gaining a stable oxygen signal at open chamber can be used to thermally equilibrate the fluorescence sensors.<br />
# After the chambers are closed and a visual check showed no bubbles (remove the fluorescence sensors from the chamber window for this), switch off the O2k chamber light and start the experiment. You will probable want to observe both the fluorescence channels (Amp) and the oxygen channels. Some initial graph layouts are provided in the additional templates for fluorescence.<br />
<br />
=== Calibration ===<br />
Different fluorescence applications require very different calibration procedures. For some types of calibrations it will be the best approach to set marks on the "Amp raw signal" and export these to a spreadsheet. Note that for some applications (H<sub>2</sub>O<sub>2</sub> production) the slope of the fluorescence signal, not the signal itself, will be the parameter of biological importance.<br />
<br />
<br />
'''Linear Calibration'''<br />
<br />
For the application Amplex(R) (Ultra)red a calibration template is available with detailed instructions: [http://www.oroboros.at/?O2k-Fluorescence O2k-Fluorescence]. The following text provides more general guidelines.<br />
<br />
If there is a linear relationship between fluorescence emission and concentration typically a multiple-point calibration is performed, plotting the signal as a function of concentration over a wide concentration range. The obtained regression parameters (slope and intercept) may be used either in a spreadsheet program to calculate averaged concentrations or used via the DatLab calibration window to directly display concentrations. Two point calibrations can be done directly in DatLab, see [[O2k-Fluorescence LED2-Module#DatLab-Analysis| DatLab-Analysis]] for both options.<br />
For some applications calibrations may be easily done using the Titration-Injection-microPump (TIP2k).<br />
<br />
<br />
'''Data export and linear calibration:''' <nowiki>Mark stable sections on the raw signal, use or generate a template of mark names, and copy to clipboard in Marks Statistics [F2]. Copy into an Excel </nowiki>template for linear regression. This template can be modified according to the specific calibration experiment (titration volumes, concentrations, number of data points, …). Perform a linear regression of the raw signal as a function of analyte concentration. For highest accuracy, only the concentration range used in the final experiment should be included in the regression. Obtain the regression parameters (slope and intercept). <br />
<br />
=== First time users ===<br />
* Set up the instrument as described [[O2k-Fluorescence_LED2-Module#Setup_of_the_O2k-Fluorescence_LED2-Module| above.]]<br />
* Get familiar with the instrument and the software options:<br />
** With [[Fluorescence-Sensor]]s outside the chamber, use DatLab to vary the light intensities of the light source in the sensor. For the blue and green sensor make sure that you see the change in light intensity with your own eyes, but without directly looking into the light source.<br />
** Insert the Fluorescence-Senors into the chamber and make sure that you can observe the change in the Amp signal when changing the light intensity. If the signal is constant near to 10 Volts the reasons may be a too high light intensity, a too high gain or a (yellow) chamber light that is still switched on.<br />
** Play with light intensity and Amp gain and observe the changing signal in DatLab<br />
** Vary the scaling of the Amp plot<br />
* Perform a calibration experiment without biological sample for your application. Make sure you observe the expected change in signal<br />
* Run a blank experiment to access potential non-biologic effects of the substances you plan to use in your experiment on the observed fluorescence signal.<br />
<br />
== References ==<br />
* [[Hickey 2012 J Comp Physiol B|Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J Comp Physiol B 182: 91-100.]]<br />
* [[Hunter 2012 Biochem Pharmacol|Hunter FW, Wanga J, Patela R, Hsua H-L, Hickey AJ, Haya MP, Wilson WR (2012) Homologous recombination repair-dependent cytotoxicity of the benzotriazine di-N-oxide CEN-209: Comparison with other hypoxia-activated prodrugs. Biochem Pharmacol 83: 574–585.]]<br />
* [[Fasching 2011 Abstract Berlin|Fasching M, Harrison DK, Tretter L, Gnaiger E (2011) Combination of high-resolution respirometry and fluorometry for continuous monitoring of hydrogen peroxide production by mitochondria with resolution in the nanomolar range. Abstract Berlin]]. <br />
* [[Sumbalova 2011 Abstract Kagoshima|Sumbalova Z, Harrison DK, Gradl P, Fasching M, Gnaiger E (2011) Mitochondrial membrane potential, coupling control, H<sub>2</sub>O<sub>2</sub> production, and the upper limit of mitochondrial performance. Abstract Kagoshima]].<br />
<br />
See also <br />
* [[Talk:O2k-Fluorescence Module|Fluorescence Discussion]]<br />
* [[Measuring_hydrogen_peroxide]]<br />
<br />
<br> {{Technical service}}<br />
{{#set:Technical service=NO (amperometric) signal |Technical service=Fluorescence }}<br />
{{MitoPedia methods<br />
|mitopedia method=Fluorometry<br />
}}<br />
{{MitoPedia topics}}</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=O2k-Fluo_LED2-Module&diff=58063O2k-Fluo LED2-Module2014-03-17T14:49:51Z<p>Bauer Christiane: </p>
<hr />
<div>{{Mainpage orobopedia}}<br />
{{Product<br />
|description=<big>The '''O2k-Fluorescence LED2-Module'''</big> is an amperometric add-on module to the [[O2k-Core]], adding a new dimension to [[HRR]]. Optical sensors are inserted through the front window of the O2k-glass chambers, for measurement of '''hydrogen peroxide production''' ([[Amplex red]]), '''ATP production''' ([[Magnesium green]]), '''mt-membrane potential''' ([[Safranin]]), '''Ca<sup>2+</sup>''' ([[Calcium green]]), and numerous other applications open for O2k-user innovation. <br />
The O2k-Fluorescence LED2-Module consists of optical sensors for both [[O2k-Chamber]]s (LEDs for green and blue excitation), optical filters, [[Fluorescence-Control Unit]] for regulation of light intensity, data input into the [[O2k-Main Unit]], and the updated '''DatLab''' software.<br />
|product id=12100-01<br />
|product type=O2k, O2k-Module, MultiSensor, Catalogue<br />
|info=[http://www.oroboros.at/?O2k-Fluorescence O2k-Fluorescence@OROBOROS], [[Oxygraph-2k]], [[G MiPNet17.05 O2k-Fluorescence LED2-Module]]<br />
|product image=[[Image:O2k-Fluorescence LED2-Module.JPG|180px|right|link=http://www.bioblast.at/index.php/O2k-Fluorescence_LED2-Module]]<br />
}}<br />
__TOC__<br />
== The '''O2k-Fluorescence LED2-Module''' ''consists of'' ==<br />
{{#ask: mainlabel=Title|[[Category:Products]] | [[Product type::O2k-Fluorescence LED2-Module]]<br />
| ?description<br />
| ?product id<br />
| ?product image<br />
|sort=product id<br />
|order=ascending<br />
}}<br />
<br />
== O2k-Fluorometry Workshops ==<br />
* 2013 September 19-23. [http://www.oroboros.at/index.php?id=1534 '''International O2k-Fluorometry Workshop IOC79, Obergurgl, Austria'''].<br />
* 2012 March [http://www.oroboros.at/?IOC66 O2k-Fluorometry Workshop, March 2012, Innsbruck, Austria.] <br />
* [http://www.oroboros.at/?O2k-Workshops | upcoming Workshops]<br />
<br />
<br />
== O2k-Manual: O2k-Fluorescence LED2-Module ==<br />
>> [[G MiPNet17.05 O2k-Fluorescence LED2-Module|O2k-Manual: O2k-Fluorescence LED2-Module]]<br />
: Open Innovation: The '''''Manual'' for the O2k-Fluorescence LED2-Module''' evolves as a guided tour through the '''O2k-Catalogue: O2k-Fluorescence LED2-Module'''.<br />
<br />
=== Scope of Application ===<br />
There are many different fluorometric measurement techniques. The O2k-Fluorescence LED2-Module is designed for measurements that are typically performed by placing a (stirred) cuvette with the sample into a (spectro)fluormeter. Protocols for these cuvette based measurements can be implemented with the O2k-Fluorescence LED2-Module, adding simultaneous measurement of respiration and the monitoring of oxygen concentration to the protocol. The scope of possible applications is further defined by the excitation wavelengths provided by the sensors Fluorescence-Sensor Green and Fluorescence-Sensor Blue and the availability of suitable emission filters.<br />
Therefore, classical fluorometric measurements can be “translated” to an O2k equipped with the O2k-Fluorescence LED2-Module if the following conditions are met:<br />
* the fluorescence measurement was done in a cuvette using a (spectro)fluorometer<br />
* one of the fluorophores listed below under [[O2k-Fluorescence_LED2-Module#Application-specific_settings]] was used OR excitation and emission wavelength of the fluorophore are suitable for use with the O2k-Fluorescence LED2-Module. Please contact Oroboros Instruments if in doubt about the latter point. <br />
<br />
The O2k-Fluorescence LED2-Module is not designed to fulfill the functions of other devises used in fluorometric measurements e.g. fluorescence microscopes, flow cytometers, etc..<br />
<br />
=== Setup of the O2k-Fluorescence LED2-Module ===<br />
# [[Fluorescence-Control_Unit#Setup_of_the_O2k-Fluorescence_LED2-Module|Setup of the O2k-Fluorescence LED2-Module]]<br />
# [[Fluorescence-Sensor#Select_the_Fluorescence-Sensors|Selecting a Fluorescence Sensor]]'''<br />
# [[Filter-Cap#Mounting_a_Filter-Cap|Mounting a Filter-Cap]]<br />
# [[Fluorescence-Sensor#Connect_Fluorescence-Sensor_to_O2k|Connect Fluorescence-Sensor to O2k-Main unit]]<br />
<br />
=== Electronic settings ===<br />
# [[Fluorescence-Control_Unit#Power_on|Power on]]<br />
# [[Fluorescence-Control_Unit#Control_of_LED-intensity|LED-intensity]]<br />
# [[Fluorescence-Control_Unit#Gain|Amplification or Gain]]<br />
<br />
=== Application-specific settings ===<br />
The light intensity of the LED ([[Fluorescence-Control_Unit#Control_of_LED-intensity|LED-intensity]]) and the signal amplification ([[Fluorescence-Control_Unit#Amplification|Gain]]) can be adjusted in a wide range. The table suggests initial values, which may be optimised for specific applications. <br />
<br />
{| class="wikitable"<br />
|-<br />
! Application !!Sensor!! Filter set !! Light intensity (polarization voltage) - ''Note a'' !! Gain<br />
|- <br />
| [[Amplex® UltraRed]]||[[Fluorescence-Sensor Green]] || [[ Filter Set AmR| AmR]] || 100 - 500 || 1000 <br /><br />
(at light intensity = 100)<br />
|- <br />
| [[TMRM]]||[[Fluorescence-Sensor Green]] || [[ Filter Set AmR| AmR]] || 500 - 1000 || 1000 <br />
|-<br />
| [[Safranin]] ||[[Fluorescence-Sensor Blue]]|| [[Filter Set Saf|Saf]] ||200 for [safranin] > 2 µM; 500 or higher for [safranin] < 2 µM|| 1000<br />
|-<br />
| [[Magnesium green]]||[[Fluorescence-Sensor Blue]]|| [[Filter Set MgG / CaG| MgG / CaG]] || 300|| ''Note b''<br />
|-<br />
| [[Calcium green]]||[[Fluorescence-Sensor Blue]]|| [[Filter Set MgG / CaG| MgG / CaG]] |||| ''Note b''<br />
|}<br />
<br />
* ''Note a'': Set the polarization voltage [mV] for the amperometric channel (Amp) in the DatLab menu [O2k-MultiSensor \ O2k Control \ Amp polarisation voltage]. Divide the polarisation voltage [mV] by 100 to obtain the current [mA] through the LED. For simple operation instructions, it is sufficient to refer to the polarization settings selected in DatLab.<br />
* ''Note b'': The amplification for Magnesium green(R) and Calcium green(R) depends on the concentration of the fluorophore, which varies drastically between different applications. Therefore, no general recommendation is given for the appropriate gain. <br />
<br />
<br />
=== [[DatLab]]-Analysis ===<br />
<br />
==== Additional Templates for Fluorescence ====<br />
A DatLab Template file for fluorescence applications can be downloaded [http://www.oroboros.at/?O2k-fluorescence @OROBOROS].<br />
See [[MiPNet12.07]] for instructions how to import a DatLab Template files. <br />
<br />
<br />
==== Observing the fluorescence signal ====<br />
Use Graph Layout “A Amp” to display “Amp Raw Signal”<br />
<br />
[[Image:DL5_graph_select_plots_amp.PNG|thumb|250px|alt=Check Amp raw signal form Graph/Select Plots to display the fluorometric signal| Graph / Select plots ]]'''Graph layout''': Three plots are available in DatLab based on the recorded signal: '''Amp Raw Signal''', '''Amp Calibrated''', and '''Amp Slope'''<nowiki>. These plots can be selected from the drop-down lines and displayed with their check boxes either on the Y1 or Y2 [Graph layout / Select Plots].</nowiki> <br />
<br />
<br />
<br />
'''Amp Raw Signal''' displays the raw voltage (including amplification) as recorded by the Oxygraph at a given gain setting. <br />
<br />
'''Amp Calibrated''' is the signal after calibration with the parameters set in the O2k-MultiSensor Calibration window. <br />
<br />
'''Amp slope''' is the time derivative of the '''calibrated''' signal, multiplied by '''1000''', in units [m(conc. Unit during calibration)/s], so if the signal was calibrated in µM [nmol/ml] the unit of the slope is pmol/(s ml). To obtain the slope of the raw signal check the appropriate box in the calibration window ([[DatLab]] 5.1.0.130 and above).<br />
<br />
'''Graphs''' can be generated to display oxygen and fluorescence data, or several graphs can be added to display oxygen and fluorescence data separately. Layout templates are provided, which can be modified and saved as appropriate. All graph settings can be saved as user-defined layouts [[MiPNet12.07]].<br />
<br />
==== The calibration window ====<br />
If the active plot is "Amp" or "Amp slope" using either [MultiSensor]/[MultiSensor Calibration], or <nowiki>[Ctrl+F5] </nowiki>, pressing the right mouse key on the plot legend will open the calibration window for the Amp channel, see [[MiPNet12.08]].<br />
<br />
'''Setting properties of calibrated signal plot and slope plot''' <br />
[[Image:DL5_1_0_130_Amp_calibration_user_settings.PNG|400px|right]]<br />
* Type the desired plot name in the field "Name for Amp-channel". Avoid long names.<br />
* Choose the desired unit for the calibrated signal from the drop down menu beside "Unit". Default: "µM". <br />
* Choose the desired factor for slope calculation from the drop down menu beside "Slope factor". Default: 1000. The correct unit for the slope will be set by DatLab depending on the chosen unit for the calibrated signal and the factor for slope calculation. Changing the factor will recalculate the values for the slope plot correctly.<br />
* To calculate the slope based on the [[raw signal]], activate the checkbox "Calculate from raw signal". If viewing the raw (uncalibrated signal) it is recommended to use this option.<br />
* Click "Calibrate and Copy to Clipboard" to apply all changes.<br />
<br />
'''Two point linear calibration:'''<br />
To perform a simple two-point linear calibration of the "Amp channel"<br />
* select "Amp" as active plot<br />
* place two marks on to stable regions of the signal corresponding to two known concentrations<br />
* open the calibration window<br />
* use the drop down menus in the "Select Mark" column to to select the two marks of known concentration<br />
* enter the concentrations in the column labeled "Amp concentration"; express the concentrations in the unit selected as described above<br />
* Click "Calibrate and Copy to Clipboard" to apply all changes. <br />
<br />
<br />
'''Multiple point linear calibration:'''<br />
<br />
# <nowiki>Calculate the regression of raw voltage [V] as a function of concentration [µM] in a spreadsheet program.</nowiki><br />
# Note slope and intercept; add the two values to get the value for "slope+intercept"<br />
# Open the MultiSensor calibration window<br />
# Enter the data matrix shown below<br />
# Press Calibrate and Copy to clipboard.<br />
[[file:DL5_Amp_multpoint_calib.PNG|300px|right]]<br />
{| class="wikitable"<br />
|-<br />
! c [µM] !! Select Mark !! Raw Signal [V]<br />
|-<br />
| 1 || leave empty || slope + intercept<br />
|-<br />
| 0 || leave empty|| intercept<br />
|}<br />
<br />
<br />
<br />
<br />
<br />
<nowiki>Alternative: If the regression was done for c in [µM] against raw voltage in [V] the following data matrix has to be entered:</nowiki><br />
<br />
{| class="wikitable"<br />
|-<br />
! c [µM] !! Select Mark !! Raw Signal [V]<br />
|-<br />
| slope + intercept || leave empty || 1<br />
|-<br />
| intercept || leave empty|| 0<br />
|}<br />
<br />
=== Performing an experiment ===<br />
The O2k-Fluorescence Sensors can be inserted into the chamber of the O2k at any time when using the O2k. <br />
<br />
# Set up the instrument as described [[O2k-Fluorescence_LED2-Module#Setup_of_the_O2k-Fluorescence_LED2-Module above.]]<br />
# Switch off the chamber light [F10].<br />
# Observe the "Amp raw signal" and "slope Amp" as described above. It will take some minutes for the sensor to reach a constant temperature and therefore a stable signal. Therefore, it is advisable to insert the fluorescence sensors early in the set up of the instrument. However, they can be removed at any time to visually check the chamber. When a sensor is removed for a short time only it will reach a stable temperature and therefore a stable signal very soon after re-inserting it into the window of the O2k chamber.<br />
# Set up your experiment as usually for respiration experiments. Remove the fluorescence sensors whenever necessary. The time for gaining a stable oxygen signal at open chamber can be used to thermally equilibrate the fluorescence sensors.<br />
# After the chambers are closed and a visual check showed no bubbles (remove the fluorescence sensors from the chamber window for this), switch off the O2k chamber light and start the experiment. You will probable want to observe both the fluorescence channels (Amp) and the oxygen channels. Some initial graph layouts are provided in the additional templates for fluorescence.<br />
<br />
=== Calibration ===<br />
Different fluorescence applications require very different calibration procedures. For some types of calibrations it will be the best approach to set marks on the "Amp raw signal" and export these to a spreadsheet. Note that for some applications (H<sub>2</sub>O<sub>2</sub> production) the slope of the fluorescence signal, not the signal itself, will be the parameter of biological importance.<br />
<br />
<br />
'''Linear Calibration'''<br />
<br />
For the application Amplex(R) (Ultra)red a calibration template is available with detailed instructions: [http://www.oroboros.at/?O2k-Fluorescence O2k-Fluorescence]. The following text provides more general guidelines.<br />
<br />
If there is a linear relationship between fluorescence emission and concentration typically a multiple-point calibration is performed, plotting the signal as a function of concentration over a wide concentration range. The obtained regression parameters (slope and intercept) may be used either in a spreadsheet program to calculate averaged concentrations or used via the DatLab calibration window to directly display concentrations. Two point calibrations can be done directly in DatLab, see [[O2k-Fluorescence LED2-Module#DatLab-Analysis| DatLab-Analysis]] for both options.<br />
For some applications calibrations may be easily done using the Titration-Injection-microPump (TIP2k).<br />
<br />
<br />
'''Data export and linear calibration:''' <nowiki>Mark stable sections on the raw signal, use or generate a template of mark names, and copy to clipboard in Marks Statistics [F2]. Copy into an Excel </nowiki>template for linear regression. This template can be modified according to the specific calibration experiment (titration volumes, concentrations, number of data points, …). Perform a linear regression of the raw signal as a function of analyte concentration. For highest accuracy, only the concentration range used in the final experiment should be included in the regression. Obtain the regression parameters (slope and intercept). <br />
<br />
=== First time users ===<br />
* Set up the instrument as described [[O2k-Fluorescence_LED2-Module#Setup_of_the_O2k-Fluorescence_LED2-Module| above.]]<br />
* Get familiar with the instrument and the software options:<br />
** With [[Fluorescence-Sensor]]s outside the chamber, use DatLab to vary the light intensities of the light source in the sensor. For the blue and green sensor make sure that you see the change in light intensity with your own eyes, but without directly looking into the light source.<br />
** Insert the Fluorescence-Senors into the chamber and make sure that you can observe the change in the Amp signal when changing the light intensity. If the signal is constant near to 10 Volts the reasons may be a too high light intensity, a too high gain or a (yellow) chamber light that is still switched on.<br />
** Play with light intensity and Amp gain and observe the changing signal in DatLab<br />
** Vary the scaling of the Amp plot<br />
* Perform a calibration experiment without biological sample for your application. Make sure you observe the expected change in signal<br />
* Run a blank experiment to access potential non-biologic effects of the substances you plan to use in your experiment on the observed fluorescence signal.<br />
<br />
== References ==<br />
* [[Hickey 2012 J Comp Physiol B|Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J Comp Physiol B 182: 91-100.]]<br />
* [[Hunter 2012 Biochem Pharmacol|Hunter FW, Wanga J, Patela R, Hsua H-L, Hickey AJ, Haya MP, Wilson WR (2012) Homologous recombination repair-dependent cytotoxicity of the benzotriazine di-N-oxide CEN-209: Comparison with other hypoxia-activated prodrugs. Biochem Pharmacol 83: 574–585.]]<br />
* [[Fasching 2011 Abstract Berlin|Fasching M, Harrison DK, Tretter L, Gnaiger E (2011) Combination of high-resolution respirometry and fluorometry for continuous monitoring of hydrogen peroxide production by mitochondria with resolution in the nanomolar range. Abstract Berlin]]. <br />
* [[Sumbalova 2011 Abstract Kagoshima|Sumbalova Z, Harrison DK, Gradl P, Fasching M, Gnaiger E (2011) Mitochondrial membrane potential, coupling control, H<sub>2</sub>O<sub>2</sub> production, and the upper limit of mitochondrial performance. Abstract Kagoshima]].<br />
<br />
See also <br />
* [[Talk:O2k-Fluorescence Module|Fluorescence Discussion]]<br />
* [[Measuring_hydrogen_peroxide]]<br />
<br />
<br> {{Technical service}}<br />
{{#set:Technical service=NO (amperometric) signal |Technical service=Fluorescence }}<br />
{{MitoPedia methods<br />
|mitopedia method=Fluorometry<br />
}}<br />
{{MitoPedia topics}}</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=O2k-Fluo_LED2-Module&diff=58062O2k-Fluo LED2-Module2014-03-17T14:49:14Z<p>Bauer Christiane: </p>
<hr />
<div>{{Mainpage orobopedia}}<br />
{{Product<br />
|description=<big>The '''O2k-Fluorescence LED2-Module'''</big> is an amperometric add-on module to the [[O2k-Core]], adding a new dimension to [[HRR]]. Optical sensors are inserted through the front window of the O2k-glass chambers, for measurement of '''hydrogen peroxide production''' ([[Amplex red]]), '''ATP production''' ([[Magnesium green]]), '''mt-membrane potential''' ([[Safranin]]), '''Ca<sup>2+</sup>''' ([[Calcium green]]), and numerous other applications open for O2k-user innovation. <br />
The O2k-Fluorescence LED2-Module consists of optical sensors for both [[O2k-Chamber]]s (LEDs for green and blue excitation), optical filters, [[Fluorescence-Control Unit]] for regulation of light intensity, data input into the [[O2k-Main Unit]], and the updated '''DatLab''' software.<br />
|product id=12100-01<br />
|product type=O2k, O2k-Module, MultiSensor, Catalogue<br />
|info=[http://www.oroboros.at/?O2k-Fluorescence O2k-Fluorescence@OROBOROS], [[Oxygraph-2k]], [[G MiPNet17.05 O2k-Fluorescence LED2-Module]]<br />
|product image=[[Image:O2k-Fluorescence LED2-Module.JPG|180px|right|link=http://www.bioblast.at/index.php/O2k-Fluorescence_LED2-Module]]<br />
}}<br />
__TOC__<br />
== The '''O2k-Fluorescence LED2-Module''' ''consists of'' ==<br />
{{#ask: mainlabel=Title|[[Category:Products]] | [[Product type::O2k-Fluorescence LED2-Module]]<br />
| ?description<br />
| ?product id<br />
| ?product image<br />
|sort=product id<br />
|order=ascending<br />
}}<br />
<br />
== O2k-Fluorometry Workshops ==<br />
* 2013 September 19-23. [http://www.oroboros.at/index.php?id=1534 '''International O2k-Fluorometry Workshop IOC79, Obergurgl, Austria'''].<br />
* 2012 March [http://www.oroboros.at/?IOC66 O2k-Fluorometry Workshop, March 2012, Innsbruck, Austria.] <br />
* [http://www.oroboros.at/?O2k-Workshops|upcoming Workshops]<br />
<br />
<br />
== O2k-Manual: O2k-Fluorescence LED2-Module ==<br />
>> [[G MiPNet17.05 O2k-Fluorescence LED2-Module|O2k-Manual: O2k-Fluorescence LED2-Module]]<br />
: Open Innovation: The '''''Manual'' for the O2k-Fluorescence LED2-Module''' evolves as a guided tour through the '''O2k-Catalogue: O2k-Fluorescence LED2-Module'''.<br />
<br />
=== Scope of Application ===<br />
There are many different fluorometric measurement techniques. The O2k-Fluorescence LED2-Module is designed for measurements that are typically performed by placing a (stirred) cuvette with the sample into a (spectro)fluormeter. Protocols for these cuvette based measurements can be implemented with the O2k-Fluorescence LED2-Module, adding simultaneous measurement of respiration and the monitoring of oxygen concentration to the protocol. The scope of possible applications is further defined by the excitation wavelengths provided by the sensors Fluorescence-Sensor Green and Fluorescence-Sensor Blue and the availability of suitable emission filters.<br />
Therefore, classical fluorometric measurements can be “translated” to an O2k equipped with the O2k-Fluorescence LED2-Module if the following conditions are met:<br />
* the fluorescence measurement was done in a cuvette using a (spectro)fluorometer<br />
* one of the fluorophores listed below under [[O2k-Fluorescence_LED2-Module#Application-specific_settings]] was used OR excitation and emission wavelength of the fluorophore are suitable for use with the O2k-Fluorescence LED2-Module. Please contact Oroboros Instruments if in doubt about the latter point. <br />
<br />
The O2k-Fluorescence LED2-Module is not designed to fulfill the functions of other devises used in fluorometric measurements e.g. fluorescence microscopes, flow cytometers, etc..<br />
<br />
=== Setup of the O2k-Fluorescence LED2-Module ===<br />
# [[Fluorescence-Control_Unit#Setup_of_the_O2k-Fluorescence_LED2-Module|Setup of the O2k-Fluorescence LED2-Module]]<br />
# [[Fluorescence-Sensor#Select_the_Fluorescence-Sensors|Selecting a Fluorescence Sensor]]'''<br />
# [[Filter-Cap#Mounting_a_Filter-Cap|Mounting a Filter-Cap]]<br />
# [[Fluorescence-Sensor#Connect_Fluorescence-Sensor_to_O2k|Connect Fluorescence-Sensor to O2k-Main unit]]<br />
<br />
=== Electronic settings ===<br />
# [[Fluorescence-Control_Unit#Power_on|Power on]]<br />
# [[Fluorescence-Control_Unit#Control_of_LED-intensity|LED-intensity]]<br />
# [[Fluorescence-Control_Unit#Gain|Amplification or Gain]]<br />
<br />
=== Application-specific settings ===<br />
The light intensity of the LED ([[Fluorescence-Control_Unit#Control_of_LED-intensity|LED-intensity]]) and the signal amplification ([[Fluorescence-Control_Unit#Amplification|Gain]]) can be adjusted in a wide range. The table suggests initial values, which may be optimised for specific applications. <br />
<br />
{| class="wikitable"<br />
|-<br />
! Application !!Sensor!! Filter set !! Light intensity (polarization voltage) - ''Note a'' !! Gain<br />
|- <br />
| [[Amplex® UltraRed]]||[[Fluorescence-Sensor Green]] || [[ Filter Set AmR| AmR]] || 100 - 500 || 1000 <br /><br />
(at light intensity = 100)<br />
|- <br />
| [[TMRM]]||[[Fluorescence-Sensor Green]] || [[ Filter Set AmR| AmR]] || 500 - 1000 || 1000 <br />
|-<br />
| [[Safranin]] ||[[Fluorescence-Sensor Blue]]|| [[Filter Set Saf|Saf]] ||200 for [safranin] > 2 µM; 500 or higher for [safranin] < 2 µM|| 1000<br />
|-<br />
| [[Magnesium green]]||[[Fluorescence-Sensor Blue]]|| [[Filter Set MgG / CaG| MgG / CaG]] || 300|| ''Note b''<br />
|-<br />
| [[Calcium green]]||[[Fluorescence-Sensor Blue]]|| [[Filter Set MgG / CaG| MgG / CaG]] |||| ''Note b''<br />
|}<br />
<br />
* ''Note a'': Set the polarization voltage [mV] for the amperometric channel (Amp) in the DatLab menu [O2k-MultiSensor \ O2k Control \ Amp polarisation voltage]. Divide the polarisation voltage [mV] by 100 to obtain the current [mA] through the LED. For simple operation instructions, it is sufficient to refer to the polarization settings selected in DatLab.<br />
* ''Note b'': The amplification for Magnesium green(R) and Calcium green(R) depends on the concentration of the fluorophore, which varies drastically between different applications. Therefore, no general recommendation is given for the appropriate gain. <br />
<br />
<br />
=== [[DatLab]]-Analysis ===<br />
<br />
==== Additional Templates for Fluorescence ====<br />
A DatLab Template file for fluorescence applications can be downloaded [http://www.oroboros.at/?O2k-fluorescence @OROBOROS].<br />
See [[MiPNet12.07]] for instructions how to import a DatLab Template files. <br />
<br />
<br />
==== Observing the fluorescence signal ====<br />
Use Graph Layout “A Amp” to display “Amp Raw Signal”<br />
<br />
[[Image:DL5_graph_select_plots_amp.PNG|thumb|250px|alt=Check Amp raw signal form Graph/Select Plots to display the fluorometric signal| Graph / Select plots ]]'''Graph layout''': Three plots are available in DatLab based on the recorded signal: '''Amp Raw Signal''', '''Amp Calibrated''', and '''Amp Slope'''<nowiki>. These plots can be selected from the drop-down lines and displayed with their check boxes either on the Y1 or Y2 [Graph layout / Select Plots].</nowiki> <br />
<br />
<br />
<br />
'''Amp Raw Signal''' displays the raw voltage (including amplification) as recorded by the Oxygraph at a given gain setting. <br />
<br />
'''Amp Calibrated''' is the signal after calibration with the parameters set in the O2k-MultiSensor Calibration window. <br />
<br />
'''Amp slope''' is the time derivative of the '''calibrated''' signal, multiplied by '''1000''', in units [m(conc. Unit during calibration)/s], so if the signal was calibrated in µM [nmol/ml] the unit of the slope is pmol/(s ml). To obtain the slope of the raw signal check the appropriate box in the calibration window ([[DatLab]] 5.1.0.130 and above).<br />
<br />
'''Graphs''' can be generated to display oxygen and fluorescence data, or several graphs can be added to display oxygen and fluorescence data separately. Layout templates are provided, which can be modified and saved as appropriate. All graph settings can be saved as user-defined layouts [[MiPNet12.07]].<br />
<br />
==== The calibration window ====<br />
If the active plot is "Amp" or "Amp slope" using either [MultiSensor]/[MultiSensor Calibration], or <nowiki>[Ctrl+F5] </nowiki>, pressing the right mouse key on the plot legend will open the calibration window for the Amp channel, see [[MiPNet12.08]].<br />
<br />
'''Setting properties of calibrated signal plot and slope plot''' <br />
[[Image:DL5_1_0_130_Amp_calibration_user_settings.PNG|400px|right]]<br />
* Type the desired plot name in the field "Name for Amp-channel". Avoid long names.<br />
* Choose the desired unit for the calibrated signal from the drop down menu beside "Unit". Default: "µM". <br />
* Choose the desired factor for slope calculation from the drop down menu beside "Slope factor". Default: 1000. The correct unit for the slope will be set by DatLab depending on the chosen unit for the calibrated signal and the factor for slope calculation. Changing the factor will recalculate the values for the slope plot correctly.<br />
* To calculate the slope based on the [[raw signal]], activate the checkbox "Calculate from raw signal". If viewing the raw (uncalibrated signal) it is recommended to use this option.<br />
* Click "Calibrate and Copy to Clipboard" to apply all changes.<br />
<br />
'''Two point linear calibration:'''<br />
To perform a simple two-point linear calibration of the "Amp channel"<br />
* select "Amp" as active plot<br />
* place two marks on to stable regions of the signal corresponding to two known concentrations<br />
* open the calibration window<br />
* use the drop down menus in the "Select Mark" column to to select the two marks of known concentration<br />
* enter the concentrations in the column labeled "Amp concentration"; express the concentrations in the unit selected as described above<br />
* Click "Calibrate and Copy to Clipboard" to apply all changes. <br />
<br />
<br />
'''Multiple point linear calibration:'''<br />
<br />
# <nowiki>Calculate the regression of raw voltage [V] as a function of concentration [µM] in a spreadsheet program.</nowiki><br />
# Note slope and intercept; add the two values to get the value for "slope+intercept"<br />
# Open the MultiSensor calibration window<br />
# Enter the data matrix shown below<br />
# Press Calibrate and Copy to clipboard.<br />
[[file:DL5_Amp_multpoint_calib.PNG|300px|right]]<br />
{| class="wikitable"<br />
|-<br />
! c [µM] !! Select Mark !! Raw Signal [V]<br />
|-<br />
| 1 || leave empty || slope + intercept<br />
|-<br />
| 0 || leave empty|| intercept<br />
|}<br />
<br />
<br />
<br />
<br />
<br />
<nowiki>Alternative: If the regression was done for c in [µM] against raw voltage in [V] the following data matrix has to be entered:</nowiki><br />
<br />
{| class="wikitable"<br />
|-<br />
! c [µM] !! Select Mark !! Raw Signal [V]<br />
|-<br />
| slope + intercept || leave empty || 1<br />
|-<br />
| intercept || leave empty|| 0<br />
|}<br />
<br />
=== Performing an experiment ===<br />
The O2k-Fluorescence Sensors can be inserted into the chamber of the O2k at any time when using the O2k. <br />
<br />
# Set up the instrument as described [[O2k-Fluorescence_LED2-Module#Setup_of_the_O2k-Fluorescence_LED2-Module above.]]<br />
# Switch off the chamber light [F10].<br />
# Observe the "Amp raw signal" and "slope Amp" as described above. It will take some minutes for the sensor to reach a constant temperature and therefore a stable signal. Therefore, it is advisable to insert the fluorescence sensors early in the set up of the instrument. However, they can be removed at any time to visually check the chamber. When a sensor is removed for a short time only it will reach a stable temperature and therefore a stable signal very soon after re-inserting it into the window of the O2k chamber.<br />
# Set up your experiment as usually for respiration experiments. Remove the fluorescence sensors whenever necessary. The time for gaining a stable oxygen signal at open chamber can be used to thermally equilibrate the fluorescence sensors.<br />
# After the chambers are closed and a visual check showed no bubbles (remove the fluorescence sensors from the chamber window for this), switch off the O2k chamber light and start the experiment. You will probable want to observe both the fluorescence channels (Amp) and the oxygen channels. Some initial graph layouts are provided in the additional templates for fluorescence.<br />
<br />
=== Calibration ===<br />
Different fluorescence applications require very different calibration procedures. For some types of calibrations it will be the best approach to set marks on the "Amp raw signal" and export these to a spreadsheet. Note that for some applications (H<sub>2</sub>O<sub>2</sub> production) the slope of the fluorescence signal, not the signal itself, will be the parameter of biological importance.<br />
<br />
<br />
'''Linear Calibration'''<br />
<br />
For the application Amplex(R) (Ultra)red a calibration template is available with detailed instructions: [http://www.oroboros.at/?O2k-Fluorescence O2k-Fluorescence]. The following text provides more general guidelines.<br />
<br />
If there is a linear relationship between fluorescence emission and concentration typically a multiple-point calibration is performed, plotting the signal as a function of concentration over a wide concentration range. The obtained regression parameters (slope and intercept) may be used either in a spreadsheet program to calculate averaged concentrations or used via the DatLab calibration window to directly display concentrations. Two point calibrations can be done directly in DatLab, see [[O2k-Fluorescence LED2-Module#DatLab-Analysis| DatLab-Analysis]] for both options.<br />
For some applications calibrations may be easily done using the Titration-Injection-microPump (TIP2k).<br />
<br />
<br />
'''Data export and linear calibration:''' <nowiki>Mark stable sections on the raw signal, use or generate a template of mark names, and copy to clipboard in Marks Statistics [F2]. Copy into an Excel </nowiki>template for linear regression. This template can be modified according to the specific calibration experiment (titration volumes, concentrations, number of data points, …). Perform a linear regression of the raw signal as a function of analyte concentration. For highest accuracy, only the concentration range used in the final experiment should be included in the regression. Obtain the regression parameters (slope and intercept). <br />
<br />
=== First time users ===<br />
* Set up the instrument as described [[O2k-Fluorescence_LED2-Module#Setup_of_the_O2k-Fluorescence_LED2-Module| above.]]<br />
* Get familiar with the instrument and the software options:<br />
** With [[Fluorescence-Sensor]]s outside the chamber, use DatLab to vary the light intensities of the light source in the sensor. For the blue and green sensor make sure that you see the change in light intensity with your own eyes, but without directly looking into the light source.<br />
** Insert the Fluorescence-Senors into the chamber and make sure that you can observe the change in the Amp signal when changing the light intensity. If the signal is constant near to 10 Volts the reasons may be a too high light intensity, a too high gain or a (yellow) chamber light that is still switched on.<br />
** Play with light intensity and Amp gain and observe the changing signal in DatLab<br />
** Vary the scaling of the Amp plot<br />
* Perform a calibration experiment without biological sample for your application. Make sure you observe the expected change in signal<br />
* Run a blank experiment to access potential non-biologic effects of the substances you plan to use in your experiment on the observed fluorescence signal.<br />
<br />
== References ==<br />
* [[Hickey 2012 J Comp Physiol B|Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J Comp Physiol B 182: 91-100.]]<br />
* [[Hunter 2012 Biochem Pharmacol|Hunter FW, Wanga J, Patela R, Hsua H-L, Hickey AJ, Haya MP, Wilson WR (2012) Homologous recombination repair-dependent cytotoxicity of the benzotriazine di-N-oxide CEN-209: Comparison with other hypoxia-activated prodrugs. Biochem Pharmacol 83: 574–585.]]<br />
* [[Fasching 2011 Abstract Berlin|Fasching M, Harrison DK, Tretter L, Gnaiger E (2011) Combination of high-resolution respirometry and fluorometry for continuous monitoring of hydrogen peroxide production by mitochondria with resolution in the nanomolar range. Abstract Berlin]]. <br />
* [[Sumbalova 2011 Abstract Kagoshima|Sumbalova Z, Harrison DK, Gradl P, Fasching M, Gnaiger E (2011) Mitochondrial membrane potential, coupling control, H<sub>2</sub>O<sub>2</sub> production, and the upper limit of mitochondrial performance. Abstract Kagoshima]].<br />
<br />
See also <br />
* [[Talk:O2k-Fluorescence Module|Fluorescence Discussion]]<br />
* [[Measuring_hydrogen_peroxide]]<br />
<br />
<br> {{Technical service}}<br />
{{#set:Technical service=NO (amperometric) signal |Technical service=Fluorescence }}<br />
{{MitoPedia methods<br />
|mitopedia method=Fluorometry<br />
}}<br />
{{MitoPedia topics}}</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=IN_Haldia_Chakrabarti_S&diff=58061IN Haldia Chakrabarti S2014-03-17T12:51:09Z<p>Bauer Christiane: </p>
<hr />
<div>{{MiPNetLab<br />
|institution=Department of Biochemistry<br />
<br />
Institute of Post-graduate Medical Education & Research IPGMER<br />
|address=244B, A J C Bose Road<br />
|area code=700020<br />
|city=Kolkata<br />
|country=India<br />
|weblink=[[Neurocon]]<br />
|Contact=Chakrabarti S<br />
|MiPNetLab=Anand S, Banerjee A, Banerjee P, Bir A, Biswas S, Bhowmick P, Chattopadhyay M, Das G, Gayen R, Ghosh A, Hena A, Karmakar J, Khemka V, Oishimayasen N, Sinha M, Sonkar VK<br />
|Status=O2k 2012-<br />
|info=[[MiP2013]], [[IOC79]], [http://www.oroboros.at/?id=ioc73_kolkata IOC73]<br />
|keyword=Neuropathology, Oxidative stress<br />
|Info=<br />
}}</div>Bauer Christianehttps://wiki.oroboros.at/index.php?title=US_CA_La_Jolla_Felding-Habermann_B&diff=58060US CA La Jolla Felding-Habermann B2014-03-17T12:39:28Z<p>Bauer Christiane: </p>
<hr />
<div>{{MiPNetLab<br />
|institution=The Scripps Research Institute, MEM 150<br />
|address=10550 North Torrey Pines Road<br />
|area code=92037<br />
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|MiPNetLab=Matsuno-Yagi A, Yagi T, Santidrian AF<br />
|Status=O2k 2011-<br />
|info=IOC89<br />
|keywords=<br />
|Info=<br />
}}</div>Bauer Christiane