Willet 2000 Biochim Biophys Acta: Difference between revisions
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{{Publication | {{Publication | ||
|title=Willet K, Detry O, Sluse FE (2000) Resistance of isolated pulmonary mitochondria during in vitro anoxia/reoxygenation. Biochim Biophys Acta 1460: 346- | |title=Willet K, Detry O, Sluse FE (2000) Resistance of isolated pulmonary mitochondria during in vitro anoxia/reoxygenation. Biochim Biophys Acta 1460:346-52. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/11106775 PMID: 11106775] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/11106775 PMID: 11106775 Open Access] | ||
|authors=Willet K, Detry O, Sluse FE | |authors=Willet K, Detry O, Sluse FE | ||
|year=2000 | |year=2000 | ||
|journal=Biochim Biophys Acta | |journal=Biochim Biophys Acta | ||
|abstract=The aim of the study was to investigate the effect of in vitro anoxia/reoxygenation on the oxidative phosphorylation of isolated lung mitochondria. Mitochondria were isolated after harvesting from fresh pig lungs flushed with Euro-Collins solution. Mitochondrial respiratory parameters were determined in isolated mitochondria before anoxia (control), after 5–45 min anoxia followed by 5 min reoxygenation, and after 25 or 40 min of in vitro incubation in order to follow the in vitro aging of mitochondria during respiratory assays. Respiratory parameters measured after anoxia/reoxygenation did not show any oxidative phosphorylation dysfunction, indicating a high resistance of pulmonary mitochondria to in vitro anoxia/reoxygenation (up to 45 min anoxia). These results indicate that mitochondria are not directly responsible of their oxidative phosphorylation damage observed after in vivo ischemia (K. Willet et al., Transplantation 69 (2000) 582) but are a target of others cellular injuries leading to mitochondrial dysfunction in vivo. | |abstract=The aim of the study was to investigate the effect of ''in vitro'' anoxia/reoxygenation on the oxidative phosphorylation of isolated lung mitochondria. Mitochondria were isolated after harvesting from fresh pig lungs flushed with Euro-Collins solution. Mitochondrial respiratory parameters were determined in isolated mitochondria before anoxia (control), after 5–45 min anoxia followed by 5 min reoxygenation, and after 25 or 40 min of ''in vitro'' incubation in order to follow the ''in vitro'' aging of mitochondria during respiratory assays. Respiratory parameters measured after anoxia/reoxygenation did not show any oxidative phosphorylation dysfunction, indicating a high resistance of pulmonary mitochondria to ''in vitro'' anoxia/reoxygenation (up to 45 min anoxia). These results indicate that mitochondria are not directly responsible of their oxidative phosphorylation damage observed after ''in vivo'' ischemia (K. Willet et al., Transplantation 69 (2000) 582) but are a target of others cellular injuries leading to mitochondrial dysfunction ''in vivo''. | ||
|keywords=Mitochondria, Anoxia, Reoxygenation, Oxidative phosphorylation, Transplantation, (Lung) | |keywords=Mitochondria, Anoxia, Reoxygenation, Oxidative phosphorylation, Transplantation, (Lung) | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |||
|organism=Pig | |||
|tissues=Lung;gill | |||
|preparations=Isolated mitochondria | |||
|injuries=Ischemia-reperfusion | |||
|couplingstates=OXPHOS | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} | ||
Latest revision as of 11:43, 16 June 2015
| Willet K, Detry O, Sluse FE (2000) Resistance of isolated pulmonary mitochondria during in vitro anoxia/reoxygenation. Biochim Biophys Acta 1460:346-52. |
Willet K, Detry O, Sluse FE (2000) Biochim Biophys Acta
Abstract: The aim of the study was to investigate the effect of in vitro anoxia/reoxygenation on the oxidative phosphorylation of isolated lung mitochondria. Mitochondria were isolated after harvesting from fresh pig lungs flushed with Euro-Collins solution. Mitochondrial respiratory parameters were determined in isolated mitochondria before anoxia (control), after 5–45 min anoxia followed by 5 min reoxygenation, and after 25 or 40 min of in vitro incubation in order to follow the in vitro aging of mitochondria during respiratory assays. Respiratory parameters measured after anoxia/reoxygenation did not show any oxidative phosphorylation dysfunction, indicating a high resistance of pulmonary mitochondria to in vitro anoxia/reoxygenation (up to 45 min anoxia). These results indicate that mitochondria are not directly responsible of their oxidative phosphorylation damage observed after in vivo ischemia (K. Willet et al., Transplantation 69 (2000) 582) but are a target of others cellular injuries leading to mitochondrial dysfunction in vivo. • Keywords: Mitochondria, Anoxia, Reoxygenation, Oxidative phosphorylation, Transplantation, (Lung)
Labels: MiParea: Respiration
Stress:Ischemia-reperfusion Organism: Pig Tissue;cell: Lung;gill Preparation: Isolated mitochondria
Coupling state: OXPHOS
HRR: Oxygraph-2k
