Template:200 mM ADP MgG: Difference between revisions
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::: Merck (former Calbiochem): 117105 (Adenosine 5ΚΉ-Diphosphate, Potassium Salt), store at -20Β°C. | |||
::::# Weigh 1.0026 g, dilute in H<sub>2</sub>O | ::::# Weigh 1.0026 g, dilute in H<sub>2</sub>O |
Latest revision as of 13:19, 3 September 2020
- Merck (former Calbiochem): 117105 (Adenosine 5ΚΉ-Diphosphate, Potassium Salt), store at -20Β°C.
- Weigh 1.0026 g, dilute in H2O
- Adjust pH to 6.9 with KOH, preferably on ice (with pHmeter calibrated on the same condition)
- Complete with H2O to 10 mL
- Aliquote (200 Β΅L) and store at -20Β°C.
- Avoid thawing and re-freezing the aliquotes.
- The concentration can be corrected by measuring the absorbance at 260 nm and using an extinction coefficient factor of Ξ΅M = 15400 M-1β cm-1