Siekevitz 1956 J Biophys Biochem Cytol-I: Difference between revisions
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Revision as of 18:03, 25 February 2013
Siekevitz P, Watson ML (1956) Cytochemical studies of mitochondria I. The separation and identification of a membrane fraction from isolated mitochondria. J Biophys Biochem Cytol 2: 639-651. |
Siekevitz P, Watson ML (1956) J Biophys Biochem Cytol
Abstract: Mitochondria isolated from rat liver and suspended in 0.44 M sucrose were disrupted by treatment with 0.3 per cent Na deoxycholate. The treated suspension was fractionated by differential centrifugation into a number of fractions and the respective pellets were examined in sections in the electron microscope. One of these fractions was found to consist of apparently membrane-bound (vesicular) elements. The difference between interfaces and membranes was discussed and the material of this fraction was found to meet stated requirements identifying it as membranous. A detailed study of the disruption process undergone by mitochondria in the presence of Na deoxycholate showed that the elements of this fraction were derived from structural elements assumed to be mitochondrial membranes. The findings thus demonstrate that mitochondria do possess membranes as defined and that these membranes can be isolated in a relatively pure form. โข Keywords: Mitochondrial structure, mitochondrial membranes, vesicles
Labels:
Organism: Rat
Tissue;cell: Liver
Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Made history