Rotenone

high-resolution terminology - matching measurements at high-resolution

Rotenone

Description

(2R,6aS,12aS)-1,2,6,6a,12,12a-hexahydro-2-isopropenyl-8,9 dimethoxychromeno[3,4-b]furo(2,3-h)chromen-6-on; C₂₃H₂₂O₆. Inhibitor of complex I via inhibition of NADH oxidation.It inhibits the transfer of electrons from iron-sulfur centers in CI to ubiquinone via binding to the ubiquinone binding site of CI. See also S_(Rot).

Abbreviation: Rot

Reference: MiPNet11.09, MiPNet09.12, MiPNet12.15, MiPNet03.02

Labels:

Enzyme: Complex I

HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.

Application in HRR

Rot: Rotenone (Rotenone ≥95%, C₂₃H₂₂O₆); Sigma R 8875, 1 g, store at R.T.: FW = 394.42)

Caution: Very toxic and light sensitive (store solution in a dark glass vial).

Preparation of 0.1 mM ^[1] Rotenone solution (dissolved in 99.9% Ethanol):

1) weigh 0.39 mg of Rotenone directly into a dark glass vial.

2) add 10 ml Ethanol

3) store frozen at -20 °C

Oxygraph-2k Manual Titrations: MiPNet09.12

Titration volume: 1 µl using a 10 µl syringe
Final conc. in 2 ml O2k-chamber: 0.5 µM

↑ Note: Up to 2006, rotentone was added at a higher final concentration 0.5 µM), hence a 0.5 mM stock solution was prepared. Since 0.05 µM may be fully inhibiting, we suggest to use a stock solution at lower concentration (0.1 mM), to reduce the problem or rotenone retention.

[1] Note: Up to 2006, rotentone was added at a higher final concentration 0.5 µM), hence a 0.5 mM stock solution was prepared. Since 0.05 µM may be fully inhibiting, we suggest to use a stock solution at lower concentration (0.1 mM), to reduce the problem or rotenone retention.

[1]