Rotenone: Difference between revisions
No edit summary |
No edit summary |
||
| Line 20: | Line 20: | ||
'''Preparation of 0.1 mM <ref>Note</ref> Rotenone solution''' (dissolved in 99.9% Ethanol): | '''Preparation of 0.1 mM <ref>Note: Up to 2006, rotentone was added at a higher final concentration 0.5 µM), hence a 0.5 mM stock solution was prepared. Since 0.05 µM may be fully inhibiting, we suggest to use a stock solution at lower concentration (0.1 mM), to reduce the problem or rotenone retention.</ref> Rotenone solution''' (dissolved in 99.9% Ethanol): | ||
::1) weigh 0.39 mg of Rotenone directly into a dark glass vial | ::1) weigh 0.39 mg of Rotenone directly into a dark glass vial. | ||
::2) add 10 ml Ethanol | ::2) add 10 ml Ethanol | ||
::3) store frozen at -20 °C | ::3) store frozen at -20 °C | ||
| Line 31: | Line 31: | ||
::* Final conc. in 2 ml O2k-chamber: 0.5 µM | ::* Final conc. in 2 ml O2k-chamber: 0.5 µM | ||
<references/> | <references/> | ||
Revision as of 12:07, 22 November 2010
- high-resolution terminology - matching measurements at high-resolution
Rotenone
Description
(2R,6aS,12aS)-1,2,6,6a,12,12a-hexahydro-2-isopropenyl-8,9 dimethoxychromeno[3,4-b]furo(2,3-h)chromen-6-on; C23H22O6. Inhibitor of complex I via inhibition of NADH oxidation.It inhibits the transfer of electrons from iron-sulfur centers in CI to ubiquinone via binding to the ubiquinone binding site of CI. See also S(Rot).
Abbreviation: Rot
Reference: MiPNet11.09, MiPNet09.12, MiPNet12.15, MiPNet03.02
Labels:
Enzyme: Complex I
HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Application in HRR
Rot: Rotenone (Rotenone ≥95%, C23H22O6); Sigma R 8875, 1 g, store at R.T.: FW = 394.42)
Caution: Very toxic and light sensitive (store solution in a dark glass vial).
Preparation of 0.1 mM [1] Rotenone solution (dissolved in 99.9% Ethanol):
- 1) weigh 0.39 mg of Rotenone directly into a dark glass vial.
- 2) add 10 ml Ethanol
- 3) store frozen at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe
- Final conc. in 2 ml O2k-chamber: 0.5 µM
- ↑ Note: Up to 2006, rotentone was added at a higher final concentration 0.5 µM), hence a 0.5 mM stock solution was prepared. Since 0.05 µM may be fully inhibiting, we suggest to use a stock solution at lower concentration (0.1 mM), to reduce the problem or rotenone retention.
