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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
| Country | City | Contact |
|---|---|---|
| Armenia | Yerevan | Trchounian K |
| Austria | Innsbruck | Cardoso Luiza HD |
| Austria | Innsbruck | Garcia-Souza Luiz F |
| Austria | Innsbruck | Doerrier Carolina |
| Austria | Innsbruck | Karabatsiakis Alexander |
| Austria | Innsbruck | Fischer Christine |
| Austria | Innsbruck | Hoppel Florian |
| Austria | Innsbruck | Meszaros Andras T |
| Austria | Innsbruck | Torres-Quesada Omar |
| Austria | Innsbruck | Gnaiger Erich |
| Belgium | Namur | Arnould Thierry |
| Belgium | Liege | Votion Dominique-Marie |
| Belgium | Namur | Renard P |
| Belgium | Namur | Detraux Damien |
| Belgium | Leuven | Bird Matthew |
| Bosnia and Herzegovina | Tuzla | Jusic Amela |
| Bosnia and Herzegovina | Tuzla | Hajrulahovic Anesa |
| Brazil | Sao Paulo | Alencar Mayke Bezerra |
| Brazil | São Paulo | Crispim Marcell |
| Brazil | Sao Paulo | Verdaguer Ignasi Bofill |
| Brazil | Sao Paulo | Dominguez-Amorocho OA |
| Croatia | Zagreb | Rubelj Ivica |
| Czech Republic | Pilsen | Kripnerova M |
| Czech Republic | Pilsen | Markova Michaela |
| Czech Republic | Prague | Elkalaf Moustafa |
| Czech Republic | Hradec Kralove | Vernerova Andrea |
| Czech Republic | Prague | Fisar Zdenek |
| Czech Republic | Pilsen | Kuncova J |
| Czech Republic | Hradec Kralove | Maseko Tumisang Edward |
| Czech Republic | Prague 4 | Bajzikova Martina |
| Czech Republic | Prague | Pecina Petr |
| Czech Republic | Prague | Mracek Tomas |
| Czech Republic | Prague | Hansikova Hana |
| Czech Republic | Vestec | Zobalova Renata |
| Czech Republic and Australia | Prague and Southport | Neuzil Jiri |
| Czech rep | Hradec Kralove | Falaye T |
| Denmark | Hvidovre | Nehlin Jan O |
| Denmark | Aarhus | Rattan Suresh IS |
| Denmark | Malov | Hey-Mogensen M |
| Denmark | Copenhagen N | Mortensen OH |
| Denmark | Copenhagen | Rasmussen LJ |
| Denmark | Copenhagen | Desler C |
| Egypt | Cairo | Ali Sameh S |
| Egypt | Cairo | Abdel-Rahman Engy Ali |
| Estonia | Tallinn | Klepinina Lyudmila |
| Estonia | Tartu | Grellier T |
| Estonia | Tallinn | Truu Laura |
| Finland | Jyvaeskylae | Lehti Maarit |
| Finland | Turku | Stier Antoine |
| Finland | Helsinki | Pirinen E |
| France | Paris | Petit Patrice X |
| France | Bordeaux cedex | Mourier A |
| France | Bron | Rizwan Alam M |
| Germany | Mainz | Vujacic-Mirski Ksenija |
| Germany | Giessen | Eckert Gunter P |
| Germany | Munich | Jastroch M |
| Germany | Mainz | Kalinovic S |
| Germany | Magdeburg | Vieira Ligo Teixeira Camila |
| Germany | Biberach an der Riß | Nold V |
| Germany | Hamburg | Singer Dominique |
| Germany | Freising | Schoettl T |
| Germany | Duesseldorf | Eckers A |
| Germany | Ulm | Schaefer PM |
| Germany | Freising-Weihenstephan | Musiol Eva |
| Germany | Ulm | Calzia Enrico |
| Germany | Ulm | Hartmann C |
| Germany | Neuherberg | Borchard Sabine |
| Germany | Ulm | Kolassa Iris |
| Germany | Freising | Klingenspor Martin |
| Germany | Gießen | Silaidos Carmina |
| Germany | Leipzig | Ost Mario |
| Germany | Düsseldorf | Prigione Alessandro |
| Germany | Duesseldorf | Piel Sarah |
| Germany | Regensburg | Renner-Sattler Kathrin |
| Germany | Freising | Keppner Gloria |
| Germany | Freising | Fromme Tobias |
| Greece | Athens | Trougakos Ioannis P |
| Hungary | Budapest | Tretter Laszlo |
| Hungary | Budapest | Komlodi Timea |
| Hungary | Debrecen | Verebne Tar K |
| Ireland | Dublin | Karavyraki Marilena |
| Ireland | Sligo | Murphy J |
| Ireland | Dublin | Porter Richard K |
| Israel | Rishon Le Zion | Hachmo Yafit |
| Israel | Zrifin | Sova Marina |
| Israel | Jerusalem | Saada Reisch Ann |
| Italy | Bologna | Paterlini S |
| Italy | Bolzano/Bozen | Volani Chiara |
| Italy | Ancona | Battino Maurizio |
| Italy | Bologna | Porcelli AM |
| Italy | Rome | Scatena Roberto |
| Italy | Padova | Fernandez-Vizarra Erika |
| Italy | Bologna | Gasparre G |
| Italy | Bolzano | Castelo Rueda Maria Paulina |
| Italy | Milano | Bottoni P |
| Italy | Verona | Calabria Elisa |
| Italy | Bologna | Genova Maria Luisa |
| Italy | Bolzano | Pichler Irene |
| Japan | Sapporo | Shirakawa R |
| Latvia | Riga | Makarova Elina |
| Latvia | Riga | Kidere Dita |
| Malaysia | Kuala Lumpur | Abdul Karim Norwahidah |
| Malta | Msida | Vella Joanna |
| Netherlands | Wageningen | Keijer Jaap |
| Netherlands | Groningen | Rots MG |
| Netherlands | Nijmegen | Koopman Werner JH |
| Norway | Oslo | Nikolic N |
| Norway | Oslo | Rustan AC |
| Norway | Bergen | Tronstad Karl Johan |
| Norway | Oslo | Eide L |
| Norway | Oslo | Lund Jenny |
| Palestine | Ramallah | Stiban Johnny |
| Poland | Poznan | Swiniuch Daria |
| Poland | Warsaw | Dymkowska Dorota |
| Poland | Warsaw | Kampa Rafal Pawel |
| Poland | Warsaw | Sek Aleksandra |
| Poland | Poznan | Rybacka-Mossakowska Joanna |
| Poland | Lodz | Watala Cezary |
| Poland | Lodz | Labieniec-Watala Magdalena |
| Poland | Lodz | Siewiera Karolina |
| Poland | Warsaw | Wasilewski M |
| Poland | Poznań | Ludziejewska A |
| Poland | Krakow | Kaczara P |
| Poland | Poznan | Michalak Slawomir |
| Portugal | Porto | Moreira Bruno P |
| Portugal | Cantanhede | Bento Guida Jose Freitas |
| Portugal | Coimbra | Palmeira Carlos |
| Portugal | Porto | Oliveira Pedro Fontes |
| Portugal | Covilhã | Dias Tania R |
| Portugal | Porto | Bernardino RL |
| Portugal | Porto | Alves Marco G |
| Portugal | Coimbra | Pereira Susana P |
| Portugal | Cantanhede | Pinho Sonia A |
| Portugal | Porto | Beleza Jorge |
| Portugal | Catanhede | Pereira da Silva Grilo da Silva Filomena |
| Portugal | Coimbra | Carvalho Eugenia |
| Portugal | Porto | Carrageta D |
| Portugal | Porto | Magalhaes Jose |
| Portugal | Porto | Martins Ana Dias |
| Portugal | Porto | Crisostomo Luis |
| Republic of Korea | Daejeon | Park SH |
| Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
| Republic of Serbia | Belgrade | Pavlovic Kasja |
| Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
| Romania | Timisoara | Betiu AM |
| Romania | Timisoara | Avram Vlad F |
| Romania | Timisoara | Muntean Danina M |
| Romania | Timisoara | Danila Maria-Daniela |
| Romania | Timisoara | Lighezan R |
| Romania | Timisoara | Lelcu Theia |
| Russia | Moscow | Tsygankova P |
| Russia | Moscow | Krylova T |
| Russia | Moscow | Itkis YS |
| Saudi Arabia | Giza | Kotb IA |
| Serbia | Belgrad | Markovic Ivanka |
| Serbia | Belgrade | Misirlic-Dencic S |
| Serbia | Belgrade | Jeremic M |
| Serbia | Belgrade | Isakovic A |
| Serbia | Belgrade | Ivanovic Tijana |
| Serbia | Belgrade | Vukojevic M |
| Slovakia | Bratislava | Ukropcova B |
| Slovakia | Bratislava | Ukropec J |
| Slovakia | Bratislava | Maderova D |
| Slovakia | Bratislava | Nemec Michal |
| Slovakia | Bratislava | Sumbalova Zuzana |
| Slovakia | Košice | Cizmarova Beata |
| Slovenia | Ljubljana | Pirkmajer Sergej |
| Slovenia | Ljubljana | Mars T |
| Slovenia | Ljubljana | Kopitar-Jerala Natasa |
| Spain | Ourense | Reboredo-Rodriguez Patricia |
| Spain | Madrid | Lopez MG |
| Spain | Lleida | Gonzalo Hugo |
| Spain | Madrid | Cadenas S |
| Spain | Lleida | Nogueras L |
| Spain | Valencia | Borras Consuelo |
| Spain | Barcelona | Dahdah Norma Ramon |
| Spain | Barcelona | Gonzalez Casacuberta I |
| Spain | Valencia | Casado Pinna Marta |
| Spain | Granada | Quiles JL |
| Spain | Valencia | Fuertes Agudo Marina |
| Spain | Sevilla | Muntane Jordi |
| Spain | Barcelona | Juarez D |
| Spain | Barcelona | Zorzano Antonio |
| Spain | A Coruña | Mayan MD |
| Spain | Barcelona | Tura Ceide O |
| Spain | Madrid | De los Rios C |
| Spain | Sevilla | Bustos Matilde |
| Spain | Salamanca | Almeida Angeles |
| Spain | Barcelona | Gama Perez Pau |
| Spain | Armilla, Granada | Escames Germaine |
| Spain | Barcelona | Arago Marc |
| Spain | Barcelona | Hyrossova Petra |
| Spain | Barcelona | Smolders V |
| Spain | Madrid | Cano-Abad MF |
| Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
| Spain | Barcelona | Gil J |
| Spain | A Coruña | Blanco FJ |
| Spain | Madrid | Monsalve Maria |
| Spain | Valencia | Victor Victor Manuel |
| Sweden | Lund | Simon Serrano Sonia |
| Sweden | Lund | Westerlund E |
| Sweden | Lund | Leffler M |
| Sweden | Lund | Karlsson M |
| Sweden | Lund | Abliva |
| Sweden | Lund | Elmer Eskil |
| Sweden | Linköping | Fontes-Oliveira CC |
| Sweden | Lund | Chamkha Imen |
| Sweden | Södertälje | Alfaro-Moreno E |
| Sweden, SE | Lund | Aasander Frostner Eleonor |
| Switzerland | Lausanne | Zanou Nadege |
| Switzerland | Geneva | Martinou JC |
| The Netherlands | BT Amsterdam | Wuest Rob CI |
| Turkey | Ankara | Tuncay Erkan |
| Turkey | Istanbul | Baykal AT |
| Turkey | Tekirdag | Aral Cenk |
| Turkey | Izmir | Aydin HH |
| Turkey | Ankara | Engin Ayse Basak |
| USA | Fayetteville | Rao RR |
| USA | Philadelphia | Kilbaugh Todd |
| USA | Baton Rouge | Irving Brian A |
| USA | Orlando | Coen Paul M |
| USA | Fayetteville | Iyer Shilpa |
| USA | Philadelphia | Starr Jonathan |
| USA | Philadelphia | Janowska Joanna |
| USA | St Louis | Welch R |
| USA | Iowa City | Buettner Garry R |
| United Kingdom | Stoke-on-Trent | McStay Gavin P |
| United Kingdom | Leicester | Moisoi Nicoleta |
| United Kingdom | Birmingham | Thakker A |
| United Kingdom | Birmingham | Joanisse S |
| United Kingdom | Brighton | Moore Anthony L |
| United Kingdom | Oxford | Makrecka-Kuka Marina |
| Uruguay | Montevideo | Radi R |
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.
Feedback WG4
- Dr. Ali will work within the realms of WG4 (blood and cultured cells) and potentially WG1 (protocol development) as well as WG5 (dissemination and training). He will participate in data generation, quality control, and repository efforts of protocols recommended by these working groups. Dr. Ali will contribute to training workshops and conferences that aim at defining standard procedures and terminology in the field of mitochondria physiology. - Sameh S Ali (2016).
- I am very interested in being part of the management committee either as a MC member for managment tasks or in working group 4 MITOEAGLE data repository for blood cells and cultured cells. I have experience in analyzing mitochondrial function in different human tissues as cultured cells and blood cells (also subsets of specific blood populations, see list of publications), muscle and isolated mitochondria. This together with my experience in organizing summer schools and conferences could be helpful as MC member taking care of management tasks. However, if you think my expertise is more helpful in working group 4, I am looking forward to support this working group. - Kathrin Renner-Sattler (2016).
