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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
| Country | City | Contact |
|---|---|---|
| Armenia | Yerevan | Trchounian K |
| Austria | Innsbruck | Doerrier Carolina |
| Austria | Innsbruck | Karabatsiakis Alexander |
| Austria | Innsbruck | Cardoso Luiza HD |
| Austria | Innsbruck | Hoppel Florian |
| Austria | Innsbruck | Gnaiger Erich |
| Austria | Innsbruck | Fischer Christine |
| Austria | Innsbruck | Meszaros Andras T |
| Austria | Innsbruck | Torres-Quesada Omar |
| Austria | Innsbruck | Garcia-Souza Luiz F |
| Belgium | Namur | Renard P |
| Belgium | Namur | Detraux Damien |
| Belgium | Leuven | Bird Matthew |
| Belgium | Namur | Arnould Thierry |
| Belgium | Liege | Votion Dominique-Marie |
| Bosnia and Herzegovina | Tuzla | Jusic Amela |
| Bosnia and Herzegovina | Tuzla | Hajrulahovic Anesa |
| Brazil | Sao Paulo | Alencar Mayke Bezerra |
| Brazil | São Paulo | Crispim Marcell |
| Brazil | Sao Paulo | Verdaguer Ignasi Bofill |
| Brazil | Sao Paulo | Dominguez-Amorocho OA |
| Croatia | Zagreb | Rubelj Ivica |
| Czech Republic | Pilsen | Kripnerova M |
| Czech Republic | Prague | Fisar Zdenek |
| Czech Republic | Pilsen | Kuncova J |
| Czech Republic | Prague 4 | Bajzikova Martina |
| Czech Republic | Prague | Pecina Petr |
| Czech Republic | Prague | Elkalaf Moustafa |
| Czech Republic | Hradec Kralove | Vernerova Andrea |
| Czech Republic | Hradec Kralove | Maseko Tumisang Edward |
| Czech Republic | Prague | Mracek Tomas |
| Czech Republic | Vestec | Zobalova Renata |
| Czech Republic | Prague | Hansikova Hana |
| Czech Republic | Pilsen | Markova Michaela |
| Czech Republic and Australia | Prague and Southport | Neuzil Jiri |
| Czech rep | Hradec Kralove | Falaye T |
| Denmark | Malov | Hey-Mogensen M |
| Denmark | Hvidovre | Nehlin Jan O |
| Denmark | Copenhagen N | Mortensen OH |
| Denmark | Aarhus | Rattan Suresh IS |
| Denmark | Copenhagen | Rasmussen LJ |
| Denmark | Copenhagen | Desler C |
| Egypt | Cairo | Ali Sameh S |
| Egypt | Cairo | Abdel-Rahman Engy Ali |
| Estonia | Tartu | Grellier T |
| Estonia | Tallinn | Klepinina Lyudmila |
| Estonia | Tallinn | Truu Laura |
| Finland | Turku | Stier Antoine |
| Finland | Helsinki | Pirinen E |
| Finland | Jyvaeskylae | Lehti Maarit |
| France | Bordeaux cedex | Mourier A |
| France | Paris | Petit Patrice X |
| France | Bron | Rizwan Alam M |
| Germany | Mainz | Vujacic-Mirski Ksenija |
| Germany | Freising | Schoettl T |
| Germany | Duesseldorf | Eckers A |
| Germany | Ulm | Schaefer PM |
| Germany | Ulm | Calzia Enrico |
| Germany | Mainz | Kalinovic S |
| Germany | Magdeburg | Vieira Ligo Teixeira Camila |
| Germany | Freising-Weihenstephan | Musiol Eva |
| Germany | Freising | Klingenspor Martin |
| Germany | Ulm | Hartmann C |
| Germany | Leipzig | Ost Mario |
| Germany | Duesseldorf | Piel Sarah |
| Germany | Regensburg | Renner-Sattler Kathrin |
| Germany | Neuherberg | Borchard Sabine |
| Germany | Freising | Keppner Gloria |
| Germany | Ulm | Kolassa Iris |
| Germany | Giessen | Eckert Gunter P |
| Germany | Gießen | Silaidos Carmina |
| Germany | Düsseldorf | Prigione Alessandro |
| Germany | Munich | Jastroch M |
| Germany | Biberach an der Riß | Nold V |
| Germany | Hamburg | Singer Dominique |
| Germany | Freising | Fromme Tobias |
| Greece | Athens | Trougakos Ioannis P |
| Hungary | Budapest | Komlodi Timea |
| Hungary | Debrecen | Verebne Tar K |
| Hungary | Budapest | Tretter Laszlo |
| Ireland | Dublin | Karavyraki Marilena |
| Ireland | Dublin | Porter Richard K |
| Ireland | Sligo | Murphy J |
| Israel | Rishon Le Zion | Hachmo Yafit |
| Israel | Jerusalem | Saada Reisch Ann |
| Israel | Zrifin | Sova Marina |
| Italy | Rome | Scatena Roberto |
| Italy | Bologna | Gasparre G |
| Italy | Milano | Bottoni P |
| Italy | Verona | Calabria Elisa |
| Italy | Bologna | Genova Maria Luisa |
| Italy | Padova | Fernandez-Vizarra Erika |
| Italy | Bolzano | Pichler Irene |
| Italy | Bolzano | Castelo Rueda Maria Paulina |
| Italy | Bologna | Paterlini S |
| Italy | Bolzano/Bozen | Volani Chiara |
| Italy | Ancona | Battino Maurizio |
| Italy | Bologna | Porcelli AM |
| Japan | Sapporo | Shirakawa R |
| Latvia | Riga | Makarova Elina |
| Latvia | Riga | Kidere Dita |
| Malaysia | Kuala Lumpur | Abdul Karim Norwahidah |
| Malta | Msida | Vella Joanna |
| Netherlands | Groningen | Rots MG |
| Netherlands | Nijmegen | Koopman Werner JH |
| Netherlands | Wageningen | Keijer Jaap |
| Norway | Oslo | Eide L |
| Norway | Oslo | Nikolic N |
| Norway | Oslo | Rustan AC |
| Norway | Oslo | Lund Jenny |
| Norway | Bergen | Tronstad Karl Johan |
| Palestine | Ramallah | Stiban Johnny |
| Poland | Poznan | Swiniuch Daria |
| Poland | Lodz | Watala Cezary |
| Poland | Warsaw | Kampa Rafal Pawel |
| Poland | Lodz | Labieniec-Watala Magdalena |
| Poland | Warsaw | Sek Aleksandra |
| Poland | Poznan | Rybacka-Mossakowska Joanna |
| Poland | Lodz | Siewiera Karolina |
| Poland | Warsaw | Wasilewski M |
| Poland | Krakow | Kaczara P |
| Poland | Poznan | Michalak Slawomir |
| Poland | Warsaw | Dymkowska Dorota |
| Poland | Poznań | Ludziejewska A |
| Portugal | Porto | Moreira Bruno P |
| Portugal | Cantanhede | Bento Guida Jose Freitas |
| Portugal | Porto | Oliveira Pedro Fontes |
| Portugal | Covilhã | Dias Tania R |
| Portugal | Coimbra | Carvalho Eugenia |
| Portugal | Coimbra | Pereira Susana P |
| Portugal | Porto | Bernardino RL |
| Portugal | Porto | Alves Marco G |
| Portugal | Cantanhede | Pinho Sonia A |
| Portugal | Catanhede | Pereira da Silva Grilo da Silva Filomena |
| Portugal | Porto | Beleza Jorge |
| Portugal | Porto | Carrageta D |
| Portugal | Porto | Magalhaes Jose |
| Portugal | Coimbra | Palmeira Carlos |
| Portugal | Porto | Martins Ana Dias |
| Portugal | Porto | Crisostomo Luis |
| Republic of Korea | Daejeon | Park SH |
| Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
| Republic of Serbia | Belgrade | Pavlovic Kasja |
| Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
| Romania | Timisoara | Muntean Danina M |
| Romania | Timisoara | Betiu AM |
| Romania | Timisoara | Avram Vlad F |
| Romania | Timisoara | Lighezan R |
| Romania | Timisoara | Danila Maria-Daniela |
| Romania | Timisoara | Lelcu Theia |
| Russia | Moscow | Itkis YS |
| Russia | Moscow | Tsygankova P |
| Russia | Moscow | Krylova T |
| Saudi Arabia | Giza | Kotb IA |
| Serbia | Belgrad | Markovic Ivanka |
| Serbia | Belgrade | Misirlic-Dencic S |
| Serbia | Belgrade | Isakovic A |
| Serbia | Belgrade | Ivanovic Tijana |
| Serbia | Belgrade | Jeremic M |
| Serbia | Belgrade | Vukojevic M |
| Slovakia | Bratislava | Ukropcova B |
| Slovakia | Bratislava | Sumbalova Zuzana |
| Slovakia | Bratislava | Maderova D |
| Slovakia | Bratislava | Nemec Michal |
| Slovakia | Bratislava | Ukropec J |
| Slovakia | Košice | Cizmarova Beata |
| Slovenia | Ljubljana | Pirkmajer Sergej |
| Slovenia | Ljubljana | Mars T |
| Slovenia | Ljubljana | Kopitar-Jerala Natasa |
| Spain | Ourense | Reboredo-Rodriguez Patricia |
| Spain | Barcelona | Gonzalez Casacuberta I |
| Spain | Valencia | Casado Pinna Marta |
| Spain | Lleida | Gonzalo Hugo |
| Spain | Granada | Quiles JL |
| Spain | Barcelona | Juarez D |
| Spain | Barcelona | Zorzano Antonio |
| Spain | A Coruña | Mayan MD |
| Spain | Valencia | Borras Consuelo |
| Spain | Lleida | Nogueras L |
| Spain | Barcelona | Dahdah Norma Ramon |
| Spain | Madrid | De los Rios C |
| Spain | Barcelona | Gama Perez Pau |
| Spain | Armilla, Granada | Escames Germaine |
| Spain | Valencia | Fuertes Agudo Marina |
| Spain | Sevilla | Muntane Jordi |
| Spain | Madrid | Cano-Abad MF |
| Spain | A Coruña | Blanco FJ |
| Spain | Madrid | Monsalve Maria |
| Spain | Sevilla | Bustos Matilde |
| Spain | Salamanca | Almeida Angeles |
| Spain | Barcelona | Tura Ceide O |
| Spain | Madrid | Lopez MG |
| Spain | Barcelona | Arago Marc |
| Spain | Barcelona | Hyrossova Petra |
| Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
| Spain | Madrid | Cadenas S |
| Spain | Barcelona | Smolders V |
| Spain | Barcelona | Gil J |
| Spain | Valencia | Victor Victor Manuel |
| Sweden | Lund | Karlsson M |
| Sweden | Lund | Simon Serrano Sonia |
| Sweden | Lund | Elmer Eskil |
| Sweden | Linköping | Fontes-Oliveira CC |
| Sweden | Lund | Leffler M |
| Sweden | Lund | Abliva |
| Sweden | Lund | Chamkha Imen |
| Sweden | Södertälje | Alfaro-Moreno E |
| Sweden | Lund | Westerlund E |
| Sweden, SE | Lund | Aasander Frostner Eleonor |
| Switzerland | Lausanne | Zanou Nadege |
| Switzerland | Geneva | Martinou JC |
| The Netherlands | BT Amsterdam | Wuest Rob CI |
| Turkey | Ankara | Tuncay Erkan |
| Turkey | Istanbul | Baykal AT |
| Turkey | Tekirdag | Aral Cenk |
| Turkey | Izmir | Aydin HH |
| Turkey | Ankara | Engin Ayse Basak |
| USA | Fayetteville | Iyer Shilpa |
| USA | Philadelphia | Starr Jonathan |
| USA | St Louis | Welch R |
| USA | Philadelphia | Janowska Joanna |
| USA | Fayetteville | Rao RR |
| USA | Philadelphia | Kilbaugh Todd |
| USA | Iowa City | Buettner Garry R |
| USA | Baton Rouge | Irving Brian A |
| USA | Orlando | Coen Paul M |
| United Kingdom | Birmingham | Joanisse S |
| United Kingdom | Stoke-on-Trent | McStay Gavin P |
| United Kingdom | Oxford | Makrecka-Kuka Marina |
| United Kingdom | Brighton | Moore Anthony L |
| United Kingdom | Leicester | Moisoi Nicoleta |
| United Kingdom | Birmingham | Thakker A |
| Uruguay | Montevideo | Radi R |
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.
