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Difference between revisions of "MiPNet20.12 IOC103 Manaus"

From Bioblast
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[[File:INPA-Manati 0355.JPG |240px|Manati project]]
[[File:INPA-Manati 0355.JPG |240px|Manati project]]
[[File:INPA-Science forest 0403.JPG |240px|Piranias in a traditional hut]]
[[File:INPA-Science forest 0403.JPG |240px|Piranias in a traditional hut]]
[[File:INPA-Turtles 0389.JPG |240px|Turtles in the forest]]


=== Perspectives ===
=== Perspectives ===

Revision as of 14:42, 12 July 2015

                



MiPNet20.12 IOC103 Manaus

Publications in the MiPMap
O2k-Network

Manaus BR, 2015 Jul 06-07. To the heart of the Amazon. O2k-Workshop on HRR and O2k-Fluorometry, IOC103.


OROBOROS (2015-07-06) Mitochondr Physiol Network

Abstract:

Arapaima gigas

2015 Jul 06-07. To the heart of the Amazon: comparative cardiac mitochondrial physiology of Amazon fish. O2k-Workshop on HRR and O2k-Fluorometry, IOC103.


O2k-Network Lab: BR Manaus Val AL, AT Innsbruck OROBOROS


Labels:





HRR: Oxygraph-2k 

ORO, IOC, MiPNet, 2015, Next 

Amazon, Manaus

To the heart of the Amazon

Mitochondrial function is compared in mitochondrial preparations of fish heart. According to the suggestion by Dr. Adalberto Luis Val, one of each of the major groups should be selected: Potamotrygonidae, Osteoglossidae, Characiformes, Siluriformes, Perciformes (Cichlidae). The results obtained with a basic SUIT protocol will be compared with mitochondrial respiratory control patterns from other species, including mouse and human heart mitochondria, to provide a basis for our understanding of mitochondrial respiratory control patterns in different species and tissues.

Venue and organization

BR Manaus Val AL
Entrance to LEEM

Venue

Instituto Nacional de Pesquisas de Amazonia (INPA)
Laboratório de Ecofisiologia e Evolução Molecular (LEEM)
Ministerio da Ciencia e Tecnologia
Av. Andre Araujo, 2938
69067-375 Manaus, AM, Brazil


BR Manaus Val AL

Local organizers and contact


Proceedings of the O2k-Workshop

IOC103 0331.JPG

IOC103 Manaus 2015-07-06

Day 1: Assembly of the O2k

From left to right: Erich Gnaiger, Reginaldo da Silva Oliveira, Derek Felipe Campos, Wadir Heinrichs Cardas, Ramon B Baptista.


Tambaqui 4447.JPG

Day 2: SUIT protocol for comparative mitochondrial physiology - a pilot experiment with 20 mg wet weight of cardiac tissue from Tambaqui (Colossoma macropomum). Tissue homogenate was prepared with the PBI Shredder.

Days 3-4: Project: Mitochondrial respiratory control pattern in Amazon fish heart.

High reproducibility of mitochondrial respiratory control in cardiac tissue from Tambaqui.

IOC103-Tambaqui-heart 4449.JPG IOC103-Tambaqui-heart 4451.JPG IOC103-Tambaqui-heart 4457.JPG


IOC103-intro 4444.JPG

Lecturers and tutors


Participants

IOC103 4543.JPG
IOC103 4558.JPG


SUIT protocol for the cardiac mitochondrial project

Heart homogante from various fish species will be analyzed with a basic SUIT protocol. The experimental programme is planned from July 8 to 11. 800 px 800 px

Trout heart homogenate (15 °C, 4 mg Ww/ml; 2011-10-05 A-02). Very low LEAK and ROX. No ADP limitation at D1 (1 mM) with glutamate&malate (GM), but glutamate&malate&pyruvate (GMP) was not saturated at 2 mM ADP (D2), as shown by the increase of flux when doubling the ADP concentration (4 mM; D4). No oxygen limitation. Low P/E ratio, indicating limitation of OXPHOS by the phosphorylation system. The optimum uncoupler (U; FCCP) concentration is higher than in liver homogenate (2.5 versus 1.0 µM), but 3 µM exerts already a pronounced time-dependent inhibitory effect (not due to limitation by oxygen, as shown by the lower flux after reoxygenation).

Mouse heart homogenate (37 °C) is shown for comparison.

References

  • On respiratory control of human heart mitochondria
Lemieux H, Semsroth S, Antretter H, Höfer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43:1729–38. »Bioblast link«
O2k-Workshops

Recommended IOC reading

Please click » here«

» MitoPedia: Respiratory statesOXPHOS ROUTINE ETS LEAK ROX

MitoGlobal

O2k-Workshops are listed as MitoGlobal Events.

Some impressions

INPA Science forest

Manati project Piranias in a traditional hut Turtles in the forest

Perspectives

Amazon-boat 4384.JPG Amazon-boat 4434.JPG Amazon-boat 4406.JPG Should we get a boat (Vendo - middle) for a MitoLab on the Amazon?