MiPNet18.05 Amplex-Mouse-heart

O2k-Fluorometry: HRR and H₂O₂ production in mouse cardiac tissue homogenate.

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OROBOROS (2015-06-16) Mitochondr Physiol Network

Abstract: Eigentler A, Fontana-Ayoub M, Gnaiger E (2015) O2k-Fluorometry: HRR and H₂O₂ production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(02):1-6. »Versions

The O2k-Fluorescence LED2-Module is an extension of the OROBOROS O2k for combining high-resolution respirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H₂O₂ production with Amplex® Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ETS-competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H₂O₂ production.

>> Product: OROBOROS O2k, O2k-Catalogue

>> Permeabilized muscle fibres

• O2k-Network Lab: AT Innsbruck OROBOROS, AT Innsbruck MitoCom

Labels: MiParea: Respiration

Organism: Mouse Tissue;cell: Heart Preparation: Homogenate

Regulation: Cyt c Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.

HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol

O2k-Demo, O2k-MultiSensor, Mt-Preparation, PBI-Shredder