Difference between revisions of "MiPNet17.15 PBI-Shredder Mouse-heart-brain-liver"
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{{Publication | {{Publication | ||
|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. | |title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. | ||
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/c/c9/MiPNet17.15_PBI-Shredder_Mouse-heart-brain-liver.pdf |Bioblast pdf]] | |info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/c/c9/MiPNet17.15_PBI-Shredder_Mouse-heart-brain-liver.pdf |Bioblast pdf]] | ||
|authors=OROBOROS | |authors=OROBOROS | ||
|year=2015-06-16 | |year=2015-06-16 | ||
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|preparations=Homogenate | |preparations=Homogenate | ||
|couplingstates=LEAK, OXPHOS, ETS | |couplingstates=LEAK, OXPHOS, ETS | ||
| | |pathways=N, S, NS | ||
|instruments=Oxygraph-2k, O2k-Manual, O2k-Protocol | |instruments=Oxygraph-2k, O2k-Manual, O2k-Protocol | ||
|additional=Mt-Preparation, PBI-Shredder | |additional=Mt-Preparation, PBI-Shredder | ||
Revision as of 09:27, 8 November 2016
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OROBOROS (2015-06-16) Mitochondr Physiol Network
Abstract: Eigentler A, Fontana-Ayoub M, Gnaiger E (2015) Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. Mitochondr Physiol Network 17.15(04):1-6. »Versions
A high-quality preparation of tissue homogenate may represent an optimum compromise for a variety of respirometric and fluorometric studies. These considerations provided the rationale for initiating a study with the PBI-Shredder, an auxiliary HRR-Tool providing a standardized approach to prepare homogenates of various tissues (e.g. heart, liver, brain) with high reproducibility of 100% mitochondrial yield and mitochondrial function as evaluated with HRR.
- >> O2k-Protocols:Overall contents
- >> Product: OROBOROS O2k, O2k-Catalogue
• Keywords: Tissue homogenate, Shredder
• O2k-Network Lab: AT_Innsbruck_OROBOROS, AT Innsbruck MitoCom
Labels: MiParea: Respiration, Instruments;methods
Organism: Mouse
Tissue;cell: Heart, Nervous system, Liver
Preparation: Homogenate
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
Pathway: N, S, NS
HRR: Oxygraph-2k, O2k-Manual, O2k-Protocol
Mt-Preparation, PBI-Shredder
