Laner 2016 Abstract Mito Xmas Meeting Innsbruck

Has title::OXPHOS and ET capacity in permeabilized fibers of canine superathletes.

Link:

Was written by::Laner V, Was written by::Boushel RC, Was written by::Hamilton KL, Was written by::Miller BF, Was written by::Williamson KK, Was written by::Davis MS, Was written by::Gnaiger E (Was submitted in year::2016)

Event: Was submitted to event::Mito Xmas Meeting 2016 Innsbruck AT

[[has abstract::Comparative mitochondrial physiology strongly relies on quantitative data sets for comparison of OXPHOS capacities and respiratory control patterns between species and tissues. Combination and interpretation of a wide variety of studies requires standardization of respiratory protocols, implementation of quality control criteria, and consistency of normalization. Previously we described a reference method for the application of a cytochrome c threshold as exclusion criterion in mitochondrial OXPHOS analyses [1]. Alaskan sled dogs (N=6) were studied 72 to 120 h after finishing a competitive 1,000 mile race within less than nine days. Permeabilized fibers (0.81-1.28 mg ± 0.12 SD wet weight per assay) were prepared from needle biopsies and immediately studied by high-resolution respirometry [2] using 12 chambers in parallel (Oroboros Oxygraph-2k). Compared to human skeletal muscle fibers, the canine samples were more delicate to handle, highly sticky and appeared to be fragile, disintegrating to various degrees during substrate-uncoupler-inhibitor titration (SUIT) protocols in mt-respiration medium MiR06Cr. Two substrate-uncoupler-inhibitor titration protocols were applied (Fig. 1). SUIT1 emphasized pathway control with fatty acid oxidation (F) versus carbohydrate oxidation capacity, whereas the focus of SUIT2 was on coupling control with N-linked substrates. Both protocols were designed to provide a common reference state of NS-linked ET capacity, in comparison to separate N- and S-linked pathway control states (N versus S). NS-linked ET capacity was 262±41 pmol∙s-1∙mg-1 Ww independent of the presence or absence of 0.2 mM octanoyl carnitine (F). This is the highest value so far reported for mammalian skeletal muscle. Top human endurance athletes have a NS-linked ET capacity approaching 200 pmol∙s-1∙mg-1 Ww [3], compared to 153±19 pmol∙s-1∙mg-1 Ww in competitive racing horses [4].]]

• O2k-Network Lab: Was published by MiPNetLab::AT Innsbruck MitoFit, Was published by MiPNetLab::AT Innsbruck Oroboros, Was published by MiPNetLab::CA Vancouver Boushel RC, Was published by MiPNetLab::US CO Fort Collins Miller BF, Was published by MiPNetLab::US OK Stillwater Davis MS, Was published by MiPNetLab::AT Innsbruck Gnaiger E, Was published by MiPNetLab::US CO Fort Collins Hamilton K

Labels: MiParea: MiP area::Respiration, MiP area::Comparative MiP;environmental MiP, MiP area::Exercise physiology;nutrition;life style 

Organism: Organism::Dog  Tissue;cell: tissue and cell::Skeletal muscle  Preparation: Preparation::Permeabilized tissue 

Coupling state: Coupling states::LEAK, Coupling states::OXPHOS, Coupling states::ET  Pathway: Pathways::F, Pathways::N, Pathways::S, Pathways::NS, Pathways::ROX  HRR: Instrument and method::Oxygraph-2k  Event: Event::Poster 

Affiliations

Laner V(1), Boushel RC(2), Hamilton KL(3), Miller BF(3), Williamson KK(4), Davis MS(5), Gnaiger E(1,6)

1. Oroboros Instruments Corp, Innsbruck, Austria
2. School Kinesiology, Univ British Columbia, Vancouver, BC, Canada
3. Dept Health Exercise Sc, Colorado State Univ, Fort Collins, CO, USA
4. Waypoint Veterinary Education, Edmond, OK, USA
5. Dept Physiological Sc, Oklahoma State Univ, Stillwater, OK, USA
6. Dept Visceral, Transplant Thoracic Surgery, D. Swarovski Research Lab, Medical Univ Innsbruck, Austria

References

1. Laner V, Boushel RC, Hamilton KL, Miller BF, Williamson KK, Davis MS, Gnaiger E (2014) Cytochrome c flux control factor as a quality criterion in respiratory OXPHOS analysis in canine permeabilized fibers. Mitochondr Physiol Network 19.13:63-4.
2. Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58.
3. Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41:1837-45.
4. Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7:e34890.