Keller 2016 Oxid Med Cell Longev: Difference between revisions
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{{Labeling | {{Labeling | ||
|area=Respiration, mt-Biogenesis;mt-density | |area=Respiration, mt-Biogenesis;mt-density | ||
|diseases=Diabetes | |||
|injuries=Oxidative stress;RONS | |||
|organism=Rat | |organism=Rat | ||
|tissues=Endothelial;epithelial;mesothelial cell | |tissues=Endothelial;epithelial;mesothelial cell | ||
|preparations=Permeabilized cells | |preparations=Permeabilized cells | ||
|enzymes=Complex I, Complex II;succinate dehydrogenase, Complex III | |enzymes=Complex I, Complex II;succinate dehydrogenase, Complex III | ||
|couplingstates=LEAK, OXPHOS, ET | |||
|couplingstates=LEAK, OXPHOS, | |||
|pathways=N, NS | |pathways=N, NS | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=2016-04 | |additional=2016-04 | ||
}} | }} |
Latest revision as of 14:52, 13 November 2017
Keller AC, Knaub LA, McClatchey PM, Connon CA, Bouchard R, Miller MW, Geary KE, Walker LA, Klemm DJ, Reusch JE (2016) Differential mitochondrial adaptation in primary vascular smooth muscle cells from a diabetic rat model. Oxid Med Cell Longev 2016:8524267. |
Keller AC, Knaub LA, McClatchey PM, Connon CA, Bouchard R, Miller MW, Geary KE, Walker LA, Klemm DJ, Reusch JE (2016) Oxid Med Cell Longev
Abstract: Diabetes affects more than 330 million people worldwide and causes elevated cardiovascular disease risk. Mitochondria are critical for vascular function, generate cellular reactive oxygen species (ROS), and are perturbed by diabetes, representing a novel target for therapeutics. We hypothesized that adaptive mitochondrial plasticity in response to nutrient stress would be impaired in diabetes cellular physiology via a nitric oxide synthase- (NOS-) mediated decrease in mitochondrial function. Primary smooth muscle cells (SMCs) from aorta of the nonobese, insulin resistant rat diabetes model Goto-Kakizaki (GK) and the Wistar control rat were exposed to high glucose (25โmM). At baseline, significantly greater nitric oxide evolution, ROS production, and respiratory control ratio (RCR) were observed in GK SMCs. Upon exposure to high glucose, expression of phosphorylated eNOS, uncoupled respiration, and expression of mitochondrial complexes I, II, III, and V were significantly decreased in GK SMCs (p < 0.05). Mitochondrial superoxide increased with high glucose in Wistar SMCs (p < 0.05) with no change in the GK beyond elevated baseline concentrations. Baseline comparisons show persistent metabolic perturbations in a diabetes phenotype. Overall, nutrient stress in GK SMCs caused a persistent decline in eNOS and mitochondrial function and disrupted mitochondrial plasticity, illustrating eNOS and mitochondria as potential therapeutic targets. โข Keywords: Amplex Red
โข O2k-Network Lab: US CO Denver Schauer I
Labels: MiParea: Respiration, mt-Biogenesis;mt-density
Pathology: Diabetes
Stress:Oxidative stress;RONS
Organism: Rat
Tissue;cell: Endothelial;epithelial;mesothelial cell
Preparation: Permeabilized cells
Enzyme: Complex I, Complex II;succinate dehydrogenase, Complex III
Coupling state: LEAK, OXPHOS, ET Pathway: N, NS HRR: Oxygraph-2k
2016-04