Excel templ: Protocols
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Disclaimer: This is an alpha version and page is a currently still under development. Please DO NOT consider information below in this page to be true or of any scientific value.
This page was created to provide a list and details about DatLab protocols for analysis of mitochondrial respiration.
Protocol name | D number | Step 1 | Step 2 | Step 3 | Step 4 | Step 5 | Step 6 | Step 7 | Step 8 | Step 9 | Step 10 | Step 11 | Step 12 | Step 13 | Step 14 | Step 15 | Step 16 | Step 17 | Step 18 | Step 19 | Step 20 | Number of steps |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
SUIT-001_O2_ce-pce_D003 | D003 | ce1 | 1Dig | 1PM | 2D | 2c | 3U | 4G | 5S | 6Oct | 7Rot | 8Gp | 9Ama | 10AsTm | 11Azd | 14 | ||||||
SUIT-001_O2_PBMC-PLT_D004 | D004 | ce1 | 1Dig | 1PM | 2D | 2c | 3U | 4G | 5S | (6Oct) | 7Rot | 8Gp | 9Ama | 10AsTm | 11Azd | 14 | ||||||
SUIT-001_O2_mt_D001 | D001 | 1PM | 2D | 2c | 3U | 4G | 5S | 6Oct | 7Rot | 8Gp | 9Ama | 10AsTm | 11Azd | 12 | ||||||||
SUIT-001_O2_pfi_D002 | D002 | 1PM | 2D | 2c | 3U | 4G | 5S | 6Oct | 7Rot | 8Gp | 9Ama | 10AsTm | 11Azd | 12 | ||||||||
SUIT-002_O2_ce-pce_D007 | D007 | ce1 | 1Dig | 1D | 2M | 4Oct | 3c | 4M | 5P | 6G | 7S | 8Gp | 9U | 10Rot | 11Ama | 12AsTm | 13Azd | 16 | ||||
SUIT-002_O2_ce-pce-PBMC-PLT_D007a | D007a | ce1 | 1Dig | 1D | 2M | 4Oct | 3c | 4M | 5P | 6G | 7S | 8Gp | 9U | 10Rot | 11Ama | 12AsTm | 13Azd | 16 | ||||
SUIT-002_O2_mt_D005 | D005 | 1D | 2M | 3Oct | 3c | 4M | 5P | 6G | 7S | 8Gp | 9U | 10Rot | 11Ama | 12AsTm | 13Azd | 14 | ||||||
SUIT-002_O2_pfi_D006 | D006 | 1D | 2M | 3Oct | 3c | 4M | 5P | 6G | 7S | 8Gp | 9U | 10Rot | 11Ama | 12AsTm | 13Azd | 14 | ||||||
SUIT-003_AmR_ce_D017 | D017 | ce1 | ce2P | ce3Omy | ce4U | ce5Rot | ce6S | ce7Ama | 7 | |||||||||||||
SUIT-003_AmR_ce_D058 | D058 | ce1 | ce1SOD | ce1HRP | ce1AmR | ce2Omy | ce3U | ce4Rot | ce5Ama | 8 | ||||||||||||
SUIT-003_AmR_ce_D059 | D059 | ce1 | ce1H2O | ce1MiR05 | ce1DMSO | ce2Omy | ce3U | ce4Rot | ce5Ama | 8 | ||||||||||||
SUIT-003_O2_ce_D009 | D009 | ce1 | ce2Omy | ce3U | ce4Rot | ce5Ama | 5 | |||||||||||||||
SUIT-003_O2_ce_D012 | D012 | ce1 | ce1P | ce2Omy | ce3U | ce4Rot | ce5Ama | 6 | ||||||||||||||
SUIT-003_O2_ce_D028 | D028 | ce1 | ce2Omy | ce3U | ce4Rot | ce5S | ce6Ama | 6 | ||||||||||||||
SUIT-003_O2_ce_D037 | D037 | ce1 | ce1Glc | (ce2Omy) | ce3U | ce4Ama | 5 | |||||||||||||||
SUIT-003_O2_ce_D038 | D038 | ce1 | (ce2Omy) | ce3U | ce3Glc | ce3'U | ce4Ama | 6 | ||||||||||||||
SUIT-003_O2_ce_D039 | D039 | ce1 | (ce2Omy) | ce3U | ce4Rot | ce5Ama | 5 | |||||||||||||||
SUIT-003_O2_ce_D050 | D050 | ce1 | ce1Snv | (ce2Omy) | ce3U | ce4Rot | ce5Ama | 6 | ||||||||||||||
SUIT-003_O2_ce_D060 | D060 | ce1 | (ce2Omy) | ce3U | ce4Rot | ce5Snv | ce6Mnanv | ce7Ama | 7 | |||||||||||||
SUIT-003_O2_ce_D061 | D061 | ce1 | (ce2Omy) | ce3U | ce4Rot | ce5DMSO | ce6DMSO | ce7Ama | 7 | |||||||||||||
SUIT-003_O2_ce_D062 | D062 | ce1 | ce1DMSO | (ce2Omy) | ce3U | ce4Rot | ce5Ama | 6 | ||||||||||||||
SUIT-003_O2_ce-pce_D013 | D013 | ce1 | ce1P | ce2Omy | ce3U | ce3Glc | ce3M | ce4Rot | ce5S | 1Dig | 1U | 1c | 2Ama | 3AsTm | 4Azd | 14 | ||||||
SUIT-003_O2_ce-pce_D018 | D018 | ce1 | ce1P | ce2Omy | ce3U | ce3Glc | ce4Rot | ce5S | 1Dig | 1c | 2Ama | 3AsTm | 4Azd | 12 | ||||||||
SUIT-003_O2_ce-pce_D020 | D020 | ce1 | ce1P | ce2Omy | ce3U | ce4Rot | ce5S | 1Dig | 1c | 2Ama | 3AsTm | 4Azd | 11 | |||||||||
SUIT-004_O2_pfi_D010 | D010 | 1PM | 2D | 2c | 3U | 4S | 5Rot | 6Ama | 7AsTm | 8Azd | 9 | |||||||||||
SUIT-005_O2_pfi_D011 | D011 | 1OctM | 2D | 3P | 4S | 5U | 6Rot | 7Ama | 8AsTm | 9Azd | 9 | |||||||||||
SUIT-006_AmR_mt_D048 | D048 | 1PM | 2D | 3Omy | 4U | 5Ama | 5 | |||||||||||||||
SUIT-006_Fluo_mt_D034 | D034 | 1PM | 2D | 3Omy | 4U | 5Ama | 5 | |||||||||||||||
SUIT-006_MgG_mt_D055 | D055 | 1PM | 2D | 3Cat | 4U | 5Ama | 5 | |||||||||||||||
SUIT-006_O2_ce-pce_D029 | D029 | ce1 | 1Dig | 1PM | 2D | 2c | 3Omy | 4U | 5Ama | 8 | ||||||||||||
SUIT-006_O2_mt_D022 | D022 | 1SRot | 2D | 2c | (3Omy) | 4U | 5Ama | 6 | ||||||||||||||
SUIT-006_O2_mt_D047 | D047 | 1PM | 2D | 2c | 3Omy | 4U | 5Ama | 6 | ||||||||||||||
SUIT-007_O2_ce-pce_D030 | D030 | ce1 | 1Dig | 1G | 2D | 2c | 3M | 4U | 5Ama | 8 | ||||||||||||
SUIT-008_O2_ce-pce_D025 | D025 | ce1 | 1Dig | 1PM | 2D | 2c | 3G | 4S | 5U | 6Rot | 7Ama | 8AsTm | 9Azd | 12 | ||||||||
SUIT-008_O2_mt_D026 | D026 | 1PM | 2D | 2c | 3G | 4S | 4D | 5U | 6Rot | 7Ama | 8AsTm | 9Azd | 11 | |||||||||
SUIT-008_O2_pfi_D014 | D014 | 1PM | 2D | 2c | 3G | 4S | 4D | 5U | 6Rot | 7Ama | 8AsTm | 9Azd | 11 | |||||||||
SUIT-009_AmR_ce-pce_D019 | D019 | ce1 | 1Dig | 1S | 2D | 3P | 4Rot | 5Ama | 7 | |||||||||||||
SUIT-009_AmR_mt_D021 | D021 | 1S | 2D | 3P | 4Rot | 5Ama | 5 | |||||||||||||||
SUIT-009_O2_ce-pce_D016 | D016 | ce1 | 1Dig | 1S | 2D | 3P | 4Rot | 5Ama | 7 | |||||||||||||
SUIT-009_O2_mt_D015 | D015 | 1S | 2D | 3P | 4Rot | 5Ama | 5 | |||||||||||||||
SUIT-010_O2_ce-pce_D008 | D008 | ce1 | ce2Rot | ce3S | ce4D | 1Dig | 1c | 6 | ||||||||||||||
SUIT-011_O2_pfi_D024 | D024 | 1GM | 2D | 2c | 3S | 4U | 5Rot | 6Ama | 7 | |||||||||||||
SUIT-012_O2_ce-pce_D052 | D052 | ce1 | 1Dig | 1PM | 2D | 2c | 3G | 4U | 5Ama | 8 | ||||||||||||
SUIT-012_O2_mt_D027 | D027 | 1PM | 2D | 2c | 3G | 4U | 5Ama | 6 | ||||||||||||||
SUIT-013_AmR_ce_D023 | D023 | ce1 | 1 | |||||||||||||||||||
SUIT-014_O2_pfi_D042 | D042 | 1GM | 2D | 3P | 4S | 5U | 6Rot | 7Ama | 7 | |||||||||||||
SUIT-015_O2_pti_D043 | D043 | 1OctM | 2D | 3G | 4P | 5S | 6U | 7Rot | 8Ama | 8 | ||||||||||||
SUIT-016_O2_pfi_D044 | D044 | 1OctM | 2D | 3G | 4S | 5Rot | 6Omy | 7U | 8Ama | 8 | ||||||||||||
SUIT-017_O2_mt _D046 | D046 | 1OctM | 2D | 3G | 3c | 4S | 5U | 6Rot | 7Ama | 8 | ||||||||||||
SUIT-017_O2_pfi_D049 | D049 | 1OctM | 2D | 2c | 3G | 4S | 5U | 6Rot | 7Ama | 8 | ||||||||||||
SUIT-018_AmR_mt_D031 | D031 | 1GMS | 2D | 3Ama | 3 | |||||||||||||||||
SUIT-018_AmR_mt_D040 | D040 | 1GMS | 2D | 3Ama | 3 | |||||||||||||||||
SUIT-018_AmR_mt_D041 | D041 | 1GMS | 2D | 3Ama | 3 | |||||||||||||||||
SUIT-018_O2_mt_D054 | D054 | 1GMS | 2D | 3Ama | 3 | |||||||||||||||||
SUIT-019_O2_pfi_D045 | D045 | 1PalM | 2D | 2c | 03-Oct | 4P | 5G | 6U | 7S | 8Rot | 9Ama | 10 | ||||||||||
SUIT-020_Fluo_mt_D033 | D033 | 1PM | 2D | 3G | 4S | 5Rot | 6Omy | 7U | 8Ama | 8 | ||||||||||||
SUIT-020_O2_mt_D032 | D032 | 1PM | 2D | 2c | 3G | 4S | 5Rot | 6Omy | 7U | 8Ama | 9 | |||||||||||
SUIT-021_Fluo_mt_D036 | D036 | 1GM | 2D | 3S | 4Rot | 5Omy | 6U | 7Ama | 7 | |||||||||||||
SUIT-021_O2_mt_D035 | D035 | 1GM | 2D | 2c | 3S | 4Rot | 5Omy | 6U | 7Ama | 8 | ||||||||||||
SUIT-022 O2 ce D051 | D051 | ce1 | ce2KCN | ce3SHAM | 3 | |||||||||||||||||
SUIT-023 O2 ce D053 | D053 | ce1 | ce2SHAM | ce3KCN | 3 | |||||||||||||||||
SUIT-024_O2_ce-pce_D056 | D056 | ce1 | 1Dig | 1PM | 2T | 2D | 3Omy | 4Ama | 7 | |||||||||||||
SUIT-025_O2_mt_D057 | D057 | 1D | 2M | 03-Oct | 3c | 4M | 5P | 6G | 7S | 8Rot | 9Ama | 10 | ||||||||||
SUIT-026_AmR_mt_D064 | D064 | 1S | 2Rot | 3D | 4Ama | 4 | ||||||||||||||||
SUIT-026_O2_mt_D063 | D063 | 1S | 2Rot | 3D | 3c | 4Ama | 5 | |||||||||||||||
SUIT-027_O2_ce-pce_D065 | D065 | ce1 | 1Dig | 1M | 2D | 3M | 4P | 5G | 7 | |||||||||||||
SUIT-006_Fluo_mt_D034_chemical background | D034_chemical backgr. | 0 | ||||||||||||||||||||
SUIT-020_Fluo_mt_D033_chemical_background | D033_chemical backgr. | 0 | ||||||||||||||||||||
SUIT-021_Fluo_mt_D036_chemical background | D036_chemical backgr. | 0 |
Protocol name | D number | Short name | Notes |
---|---|---|---|
SUIT-001_O2_ce-pce_D003 | D003 | RP1_pce | Doerrier 2018 Methods Mol Biol |
SUIT-001_O2_PBMC-PLT_D004 | D004 | RP1_PBMC-PLT | Doerrier 2018 Methods Mol Biol |
SUIT-001_O2_mt_D001 | D001 | RP1_mt | Doerrier 2018 Methods Mol Biol |
SUIT-001_O2_pfi_D002 | D002 | RP1_pfi | Doerrier 2018 Methods Mol Biol |
SUIT-002_O2_ce-pce_D007 | D007 | RP2_pce | A: Doerrier 2018 Methods Mol Biol |
SUIT-002_O2_ce-pce-PBMC-PLT_D007a | D007a | RP2_PBMC-PLT | A: Doerrier 2018 Methods Mol Biol |
SUIT-002_O2_mt_D005 | D005 | RP2_mt | A: Doerrier 2018 Methods Mol Biol |
SUIT-002_O2_pfi_D006 | D006 | RP2_pfi | A: Doerrier 2018 Methods Mol Biol |
SUIT-003_AmR_ce_D017 | D017 | CCP-ce_+P+S | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf |
SUIT-003_AmR_ce_D058 | D058 | CCP-ce_+AmR | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf |
SUIT-003_AmR_ce_D059 | D059 | CCP-ce_+DMSO | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf |
SUIT-003_O2_ce_D009 | D009 | CCP-ce | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf |
SUIT-003_O2_ce_D012 | D012 | CCP-ce_+P | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf |
SUIT-003_O2_ce_D028 | D028 | CCP-ce_+S | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf |
SUIT-003_O2_ce_D037 | D037 | CCP-ce_+Glc+(Omy) | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf |
SUIT-003_O2_ce_D038 | D038 | CCP-ce_+(Omy)+Glc | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf |
SUIT-003_O2_ce_D039 | D039 | CCP-ce_+(Omy) | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf |
SUIT-003_O2_ce_D050 | D050 | CCP-ce_+Snv+(Omy) | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf |
SUIT-003_O2_ce_D060 | D060 | CCP-ce_+(Omy)+Snv+Mnanv | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf |
SUIT-003_O2_ce_D061 | D061 | CCP-ce_+(Omy)+DMSO+DMSO | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf |
SUIT-003_O2_ce_D062 | D062 | CCP-ce_+DMSO+(Omy) | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf |
SUIT-003_O2_ce-pce_D013 | D013 | CCP-ce_+Glc+M+viability+CIV | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf |
SUIT-003_O2_ce-pce_D018 | D018 | CCP-ce_+Glc+viability+CIV | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf |
SUIT-003_O2_ce-pce_D020 | D020 | CCP-ce_+viability+CIV | A: Coupling control protocol, living cells »MiPNet08.09 CellRespiration Bioblast pdf |
SUIT-004_O2_pfi_D010 | D010 | RP1-short | |
SUIT-005_O2_pfi_D011 | D011 | RP2-short | A: when malate-anaplerotic activity is zero |
SUIT-006_AmR_mt_D048 | D048 | CCP-mtprep_AmR | A: Coupling control protocol, mtprep |
SUIT-006_Fluo_mt_D034 | D034 | CCP-mtprep_Fluo | A: Coupling control protocol, mtprep |
SUIT-006_MgG_mt_D055 | D055 | CCP-mtprep_MgG | A: Coupling control protocol, mtprep |
SUIT-006_O2_ce-pce_D029 | D029 | CCP-mtprep_pce | A: Coupling control protocol, mtprep |
SUIT-006_O2_mt_D022 | D022 | CCP-mtprep_Srot | A: Coupling control protocol, mtprep |
SUIT-006_O2_mt_D047 | D047 | CCP-mtprep_PM | A: Coupling control protocol, mtprep |
SUIT-007_O2_ce-pce_D030 | D030 | Glutamate anaplerosis | A: Glutamate anaplerotic pathway |
SUIT-008_O2_ce-pce_D025 | D025 | PM+G+S_OXPHOS+Rot_ET | A: Additivity between the N- and S-pathway in the Q-junction |
SUIT-008_O2_mt_D026 | D026 | PM+G+S_OXPHOS+Rot_ET_mt | A: Additivity between the N- and S-pathway in the Q-junction |
SUIT-008_O2_pfi_D014 | D014 | PM+G+S_OXPHOS+Rot_ET_pfi | A: Additivity between the N- and S-pathway in the Q-junction |
SUIT-009_AmR_ce-pce_D019 | D019 | S_OXPHOS_Rot_AmR_pce | |
SUIT-009_AmR_mt_D021 | D021 | S_OXPHOS_Rot_AmR_mt | |
SUIT-009_O2_ce-pce_D016 | D016 | S_OXPHOS_Rot_O2_pce | |
SUIT-009_O2_mt_D015 | D015 | S_OXPHOS_Rot_O2_mt | |
SUIT-010_O2_ce-pce_D008 | D008 | Digitonin test | A: Optimization of digitonin concentration for pce |
SUIT-011_O2_pfi_D024 | D024 | GM+S_OXPHOS+Rot_ET:pfi | A: Maximum mitochondrial respiratory capacity (OXPHOS with NS substrates) and coupling/pathway control |
SUIT-012_O2_ce-pce_D052 | D052 | PM+G_OXPHOS_pce | A: Coupling control (L- P- E) with NADH-linked substrates (PM and PGM) |
SUIT-012_O2_mt_D027 | D027 | PM+G_OXPHOS_mt | A: Coupling control (L- P- E) with NADH-linked substrates (PM and PGM) |
SUIT-013_AmR_ce_D023 | D023 | AmR_ROUTINE | |
SUIT-014_O2_pfi_D042 | D042 | GM+P+S_OXPHOS+Rot_ET_pfi | A: Cells or tissue types that display a preference for GM over PM to support NADH-linked respiration |
SUIT-015_O2_pti_D043 | D043 | F+G+P+S_OXPHOS+Rot_ET | A: F-pathway in LEAK state and OXPHOS state |
SUIT-016_O2_pfi_D044 | D044 | F+G+S+Rot_OXPHOS+Omy | A: F-pathway in LEAK state and OXPHOS state |
SUIT-017_O2_mt _D046 | D046 | F+G+S_OXPHOS+Rot_ET_mt | A: |
SUIT-017_O2_pfi_D049 | D049 | F+G+S_OXPHOS+Rot_ET_pfi | A: |
SUIT-018_AmR_mt_D031 | D031 | AmR_GMS_mt1 | |
SUIT-018_AmR_mt_D040 | D040 | AmR_GMS_mt2 | |
SUIT-018_AmR_mt_D041 | D041 | AmR_GMS_mt3 | |
SUIT-018_O2_mt_D054 | D054 | O2_GMS_mt4 | |
SUIT-019_O2_pfi_D045 | D045 | Pal+Oct+P+G_OXPHOS+S+Rot_ET | A: |
SUIT-020_Fluo_mt_D033 | D033 | PM+G+S+Rot_OXPHOS+Omy_Fluo | A: simultaneous determination of O2 flux and mt-membrane potential |
SUIT-020_O2_mt_D032 | D032 | PM+G+S+Rot_OXPHOS+Omy | A: simultaneous determination of O2 flux and mt-membrane potential |
SUIT-021_Fluo_mt_D036 | D036 | OXPHOS (GM+S+Rot+Omy)_Fluo | A: simultaneous determination of O2 flux and mt-membrane potential |
SUIT-021_O2_mt_D035 | D035 | OXPHOS (GM+S+Rot+Omy) | A: simultaneous determination of O2 flux and mt-membrane potential |
SUIT-022 O2 ce D051 | D051 | AOX (ce CN+SHAM) | A: Determination of the respiration due to the alternative oxidase pathway |
SUIT-023 O2 ce D053 | D053 | AOX-ce SHAM+CN | A: Determination of respiration through the CIII-CIV pathway |
SUIT-024_O2_ce-pce_D056 | D056 | ATPase (PM) | A: Determination of the presence of ATPases in mitochondrial preparations. |
SUIT-025_O2_mt_D057 | D057 | OXPHOS (F+M+P+G+S+Rot) | |
SUIT-026_AmR_mt_D064 | D064 | S+Rot_OXPHOS_AmR | |
SUIT-026_O2_mt_D063 | D063 | S+Rot_OXPHOS | |
SUIT-027_O2_ce-pce_D065 | D065 | Malate anaplerosis | A: Malate anaplerotic pathway |
SUIT-006_Fluo_mt_D034_chemical background | D034_chemical backgr. | CCP-mtprep_chemical background | A: Coupling control protocol, mtprep |
SUIT-020_Fluo_mt_D033_chemical_background | D033_chemical backgr. | PM+G+S+Rot_OXPHOS+Omy_chemical background | A: simultaneous determination of O2 flux and mt-membrane potential |
SUIT-021_Fluo_mt_D036_chemical background | D036_chemical backgr. | OXPHOS (GM+S+Rot+Omy)_chemical background | A: simultaneous determination of O2 flux and mt-membrane potential |