Christen 2013 Abstract MiP2013: Difference between revisions
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{{Abstract | {{Abstract | ||
|title=Christen F, Desrosiers V, Blier PU (2013)Thermal sensitivity of mitochondria from arctic charr heart: Mitochondrial respiration and ROS production. Mitochondr Physiol Network 18.08. | |title=Christen F, Desrosiers V, Blier PU (2013) Thermal sensitivity of mitochondria from arctic charr heart: Mitochondrial respiration and ROS production. Mitochondr Physiol Network 18.08. | ||
|info=[[File:Logo MiP2013.jpg|150px|right|MiPsociety]][[MiP2013]], [[Laner 2013 Mitochondr Physiol Network MiP2013|Book of Abstracts Open Access]] | |||
|authors=Christen F, Desrosiers V, Blier PU | |authors=Christen F, Desrosiers V, Blier PU | ||
|year=2013 | |year=2013 | ||
|event= | |event=MiPNet18.08_MiP2013 | ||
|abstract=In the context of climate change, it is of paramount importance to investigate the thermal sensitivity of aquatic ectoterms [1]. Oxidative phosphorylation in mitochondria is one of the key processes of energy production and is known to be influenced by temperature [2,3]. The aim of our study was to shed light on the specific steps of the electron transfer | |abstract=In the context of climate change, it is of paramount importance to investigate the thermal sensitivity of aquatic ectoterms [1]. Oxidative phosphorylation in mitochondria is one of the key processes of energy production and is known to be influenced by temperature [2,3]. The aim of our study was to shed light on the specific steps of the electron transfer-pathway ([[ET-pathway]]) that contribute to the adaptation of fish to temperature changes. For this purpose, we measured oxygen consumption and hydrogen peroxide production at four different temperatures (10, 15, 20 and 25Β°C) in mitochondria isolated from arctic charr heart (''Salvelinus alpinus'') raised at 10 Β°C. Activities of citrate synthase and cytochrome ''c'' oxidase (CIV) were also measured at the same temperatures. Specifically, respiration rates of Complex I and Complex II in both coupled and uncoupled states were determined separately by adding either pyruvate, malate, ADP and FCCP for Complex I or succinate, ADP and FCCP for Complex II. Moreover, respiration rates were also measured in the presence of pyruvate+malate+succinate+ADP allowing the evaluation of Complexes I+II together. | ||
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Our preliminary results showed that the Complex I and Complex II respiration rates (taken together) were higher when measured separately than when both complexes worked simultaneously. However, this difference was only significant at 15 Β°C. This may concomitantly occur with a higher reactive oxygen species production at elevated temperatures, and potentially a disruption of mitochondrial integrity. Subsequent analyses of hydrogen peroxide production, citrate synthase and CIV activity will give us further insights into the thermal sensitivity of arctic charr heart mitochondria. | |||
|keywords=Arctic charr, Topics:Effects of temperature | |||
|mipnetlab=CA Rimouski Blier PU | |mipnetlab=CA Rimouski Blier PU | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, Comparative MiP;environmental MiP | |||
|injuries=Oxidative stress;RONS | |||
|organism=Other mammals, Fishes | |||
|tissues=Heart | |||
|preparations=Isolated mitochondria | |||
|enzymes=Marker enzyme | |||
|topics=Inhibitor, Temperature | |||
|couplingstates=OXPHOS, ET | |||
|pathways=N, S, CIV, NS, ROX | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
| | |additional=MiP2013, S04 | ||
}} | }} | ||
== Affiliations and author contributions == | == Affiliations and author contributions == | ||
Dept de biologie, UniversitΓ© du QuΓ©bec Γ Rimouski, | Dept de biologie, UniversitΓ© du QuΓ©bec Γ Rimouski, Canada. - Email: [email protected] | ||
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Email: [email protected] | |||
== References == | == References == |
Latest revision as of 11:16, 8 June 2020
Christen F, Desrosiers V, Blier PU (2013) Thermal sensitivity of mitochondria from arctic charr heart: Mitochondrial respiration and ROS production. Mitochondr Physiol Network 18.08. |
Link:
MiP2013, Book of Abstracts Open Access
Christen F, Desrosiers V, Blier PU (2013)
Event: MiPNet18.08_MiP2013
In the context of climate change, it is of paramount importance to investigate the thermal sensitivity of aquatic ectoterms [1]. Oxidative phosphorylation in mitochondria is one of the key processes of energy production and is known to be influenced by temperature [2,3]. The aim of our study was to shed light on the specific steps of the electron transfer-pathway (ET-pathway) that contribute to the adaptation of fish to temperature changes. For this purpose, we measured oxygen consumption and hydrogen peroxide production at four different temperatures (10, 15, 20 and 25Β°C) in mitochondria isolated from arctic charr heart (Salvelinus alpinus) raised at 10 Β°C. Activities of citrate synthase and cytochrome c oxidase (CIV) were also measured at the same temperatures. Specifically, respiration rates of Complex I and Complex II in both coupled and uncoupled states were determined separately by adding either pyruvate, malate, ADP and FCCP for Complex I or succinate, ADP and FCCP for Complex II. Moreover, respiration rates were also measured in the presence of pyruvate+malate+succinate+ADP allowing the evaluation of Complexes I+II together.
Our preliminary results showed that the Complex I and Complex II respiration rates (taken together) were higher when measured separately than when both complexes worked simultaneously. However, this difference was only significant at 15 Β°C. This may concomitantly occur with a higher reactive oxygen species production at elevated temperatures, and potentially a disruption of mitochondrial integrity. Subsequent analyses of hydrogen peroxide production, citrate synthase and CIV activity will give us further insights into the thermal sensitivity of arctic charr heart mitochondria.
β’ Keywords: Arctic charr, Topics:Effects of temperature
β’ O2k-Network Lab: CA Rimouski Blier PU
Labels: MiParea: Respiration, Comparative MiP;environmental MiP
Stress:Oxidative stress;RONS Organism: Other mammals, Fishes Tissue;cell: Heart Preparation: Isolated mitochondria Enzyme: Marker enzyme Regulation: Inhibitor, Temperature Coupling state: OXPHOS, ET Pathway: N, S, CIV, NS, ROX HRR: Oxygraph-2k
MiP2013, S04
Affiliations and author contributions
Dept de biologie, UniversitΓ© du QuΓ©bec Γ Rimouski, Canada. - Email: [email protected]
References
- PΓΆrtner HO, Knust R (2007) Climate change affects marine fishes through the oxygen limitation of thermal tolerance. Science 315: 95.
- Lemieux H, Tardif JC, Dutil JD, Blier PU (2010) Thermal sensitivity of cardiac mitochondrial metabolism in an ectothermic species from a cold environment, Atlantic wolffish (Anarhichas lupus). J Exp Mar Biol Ecol 384: 113β118.
- Pichaud N, Chatelain EH, Ballard JWO, Tanguay R, Morrow G, Blier PU (2010) Thermal sensitivity of mitochondrial metabolism in two distinct mitotypes of Drosophila simulans: evaluation of mitochondrial plasticity. J Exp Biol 213: 1665β1675.