Bobba 2015 Apoptosis: Difference between revisions
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{{Publication | {{Publication | ||
|title=Bobba A, Amadoro G, La Piana G, Calissano P, Atlante A (2014) Glycolytic enzyme upregulation and numbness of mitochondrial activity characterize the early phase of apoptosis in cerebellar granule cells. Apoptosis ahead of print. | |title=Bobba A, Amadoro G, La Piana G, Calissano P, Atlante A (2014) Glycolytic enzyme upregulation and numbness of mitochondrial activity characterize the early phase of apoptosis in cerebellar granule cells. Apoptosis ahead of print. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/25351440 PMID: 25351440] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/25351440 PMID: 25351440] | ||
|authors=Bobba A, Amadoro G, La Piana G, Calissano P, Atlante A | |authors=Bobba A, Amadoro G, La Piana G, Calissano P, Atlante A | ||
|year=2014 | |year=2014 | ||
|journal=Apoptosis | |journal=Apoptosis | ||
|abstract=Alzheimer’s disease (AD) and cancer proceed | |||
via one or more common molecular mechanisms: a metabolic | |||
shift from oxidative phosphorylation to glycolysis— | |||
corresponding to the activation of the Warburg effect— | |||
occurs in both diseases. The findings reported in this paper | |||
demonstrate that, in the early phase of apoptosis, glucose | |||
metabolism is enhanced, i.e. key proteins which internalize | |||
and metabolize glucose—glucose transporter, hexokinase | |||
and phosphofructokinase—are up-regulated, in concomitance | |||
with a parallel decrease in oxygen consumption by | |||
mitochondria and increase of L-lactate accumulation. | |||
Reversal of the glycolytic phenotype occurs in the presence | |||
of dichloroacetate, inhibitor of the pyruvate dehydrogenase | |||
kinase enzyme, which speeds up apoptosis of cerebellar | |||
granule cells, reawakening mitochondria and then modulating | |||
glycolytic enzymes. Loss of the adaptive advantage | |||
afforded by aerobic glycolysis, which occurs in the late | |||
phase of apoptosis, exacerbates the pathological processes | |||
underlying neurodegeneration, leading inevitably the cell | |||
to death. In conclusion, the data propose that both aerobic, | |||
i.e. Warburg effect, essentially due to the protective | |||
numbness of mitochondria, and anaerobic glycolysis, rather | |||
due to the mitochondrial impairment, characterize the | |||
entire time frame of apoptosis, from the early to the late | |||
phase, which mimics the development of AD. | |||
}} | |||
{{Labeling | |||
|area=Respiration, Patients | |||
|organism=Human | |||
|injuries=Cell death | |||
|diseases=Alzheimer's, Cancer | |||
|instruments=Protocol | |||
}} | }} | ||
Revision as of 16:48, 17 November 2014
| Bobba A, Amadoro G, La Piana G, Calissano P, Atlante A (2014) Glycolytic enzyme upregulation and numbness of mitochondrial activity characterize the early phase of apoptosis in cerebellar granule cells. Apoptosis ahead of print. |
Bobba A, Amadoro G, La Piana G, Calissano P, Atlante A (2014) Apoptosis
Abstract: Alzheimer’s disease (AD) and cancer proceed via one or more common molecular mechanisms: a metabolic shift from oxidative phosphorylation to glycolysis— corresponding to the activation of the Warburg effect— occurs in both diseases. The findings reported in this paper demonstrate that, in the early phase of apoptosis, glucose metabolism is enhanced, i.e. key proteins which internalize and metabolize glucose—glucose transporter, hexokinase and phosphofructokinase—are up-regulated, in concomitance with a parallel decrease in oxygen consumption by mitochondria and increase of L-lactate accumulation. Reversal of the glycolytic phenotype occurs in the presence of dichloroacetate, inhibitor of the pyruvate dehydrogenase kinase enzyme, which speeds up apoptosis of cerebellar granule cells, reawakening mitochondria and then modulating glycolytic enzymes. Loss of the adaptive advantage afforded by aerobic glycolysis, which occurs in the late phase of apoptosis, exacerbates the pathological processes underlying neurodegeneration, leading inevitably the cell to death. In conclusion, the data propose that both aerobic, i.e. Warburg effect, essentially due to the protective numbness of mitochondria, and anaerobic glycolysis, rather due to the mitochondrial impairment, characterize the entire time frame of apoptosis, from the early to the late phase, which mimics the development of AD.
Labels: MiParea: Respiration, Patients
Pathology: Alzheimer's, Cancer
Stress:Cell death
Organism: Human
HRR: Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
