Antimycin A: Difference between revisions
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:::: '''Preparation of 1 | :::: '''Preparation of 1.25 mM stock solution''' (dissolved in ethanol abs.) for '''0.5-mL O2k-chamber''': | ||
::::# Take 100 ยตL of the 5 mM stock solution. | ::::# Take 100 ยตL of the 5 mM stock solution. | ||
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== Inhibition of acyl-CoA oxidase and D-amino acid oxidase == | == Inhibition of acyl-CoA oxidase and D-amino acid oxidase == | ||
::::* Vamecq J, Schepers L, Parmentier G, Mannaerts GP (1987) Inhibition of peroxisomal fatty acyl-CoA oxidase by antimycin A. Biochem J 248:603-7. - [[Vamecq 1987 Biochem J |ยปBioblast linkยซ]] | ::::* Vamecq J, Schepers L, Parmentier G, Mannaerts GP (1987) Inhibition of peroxisomal fatty acyl-CoA oxidase by antimycin A. Biochem J 248:603-7. - [[Vamecq 1987 Biochem J |ยปBioblast linkยซ]] | ||
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|mitopedia topic=Inhibitor | |||
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Latest revision as of 13:23, 15 March 2022
Description
Antimycin A is an inhibitor of Complex III (CIII). It binds to the Qi site of CIII and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor). High concentrations of antimycin A also inhibit acyl-CoA oxidase and D-amino acid oxidase.
Abbreviation: Ama
Reference: MiPNet09.12
Application in HRR
- Ama: Antimycin A (from Streptomyces sp.; ), Sigma-Aldrich: A8674, store at -20 ยฐC, CAS: 1397-94-0, M = 540 gยทmol-1
- Hazard statements: H300, H400; fatal if swallowed, very toxic to aquatic life
- Ama: Antimycin A (from Streptomyces sp.; ), Sigma-Aldrich: A8674, store at -20 ยฐC, CAS: 1397-94-0, M = 540 gยทmol-1
- Sigma recommends to store desiccated, there is no hint to light sensitivity.
- Light sensitivity of Antimycin A is reported by another vendor: AlfaAesar.
- Preparation of 5 mM stock solution (dissolved in ethanol abs.) for 2-mL O2k-chamber:
- Weigh 5.4 mg of antimycin A into a small glass vial.
- Add 2 mL ethanol.
- Divide into 0.2 mL portions in clear glass vials.
- Store at -20 ยฐC.
- ยป O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 1 ยตL using a 10 ยตL Hamilton syringe.
- Final concentration: 2.5 ยตM.
- Preparation of 1.25 mM stock solution (dissolved in ethanol abs.) for 0.5-mL O2k-chamber:
- Take 100 ยตL of the 5 mM stock solution.
- Add 300 ยตL ethanol.
- ยป O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (0.5-mL O2k-chamber): 1 ยตL using a 10 ยตL Hamilton syringe.
- Final concentration: 2.5 ยตM.
- Microalgae applications
- For the specific case of microalgal cells it is recommended to prepare higher concentration:
- Preparation of 10 mM stock solution (dissolved in ethanol abs.)
- Weigh 10.8 mg of antimycin A and dissolve in 2 mL of ethanol abs..
- Divide into 0.2 mL portions in clear glass vials.
- Store at -20 ยฐC.
- ยป O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 8 ยตL using a 10 ยตL syringe.
- Final concentration: 40 ยตM.
Compare
- Myxothiazol - this CIII inhibitor induces less ROS production but is more expensive than Ama.
Antimycin A and ROS production
- Is hydrogen peroxide production measured after inhibition of the Electron transfer-pathway by rotenone and antimycin A of diagnostic significance? - Please, share your evidence (Email to [email protected]).
Inhibition of acyl-CoA oxidase and D-amino acid oxidase
- Vamecq J, Schepers L, Parmentier G, Mannaerts GP (1987) Inhibition of peroxisomal fatty acyl-CoA oxidase by antimycin A. Biochem J 248:603-7. - ยปBioblast linkยซ
MitoPedia topics:
Inhibitor